MD15G1259300.v1.1

Lignin degradation and detoxification of lignin-derived products

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000659
Physical Location & Seq
Reverse (-)
22025454 .. 22027897
2444 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1259300.v1.1.491

Sequence Viewer

Length: 1698 bp
ATGGCTAGCAGCTGCCTCCGGAACAGATTTTTGTGTGCGTTCGTGTTTGTTTTCTGCGGATTTCTTGGCTTAATCACTTCTCCAGCTGAAGCTGCTATAAAGAAATACCAGTTTGATGTTCAAGTAAAGAATGTGAGCAGATTGTGCCATGCAAAACCGATTGTTACTGTAAATGGAAGGTTTCCAGGGCCTACGATCTATGTCCGGGAAGGTGATAGACTTCAAATCAATGTTACAAACCATGCTCAATACAACATGTCAATTCACTGGCATGGATTGAAGCAGTACCGAAATGGTTGGGCAGATGGACCGGCTTATGTGACGCAATGTCCAATCCAGACCGGAAGTAGCTACACTTATGGCTTCAATGTGACAGGGCAAAGAGGAACTTTGTGGTGGCATGCACATATTCTTTGGCTAAGGGCTACTGTCTATGGAGCAATTGTGATCGTGCCTAAACAAGGAACTCCATTCCCATTCCCACAGCCGTATAGGGAAACTGAGATTATACTTGGAGAATGGTGGAATGTGGACGTGGAAACCTTTGTCAACAAAGCAAACAGCCTGGGATTGCCACCAAACTCCTCAGATGCTCACACCATCAATGGCAAGCCAGGGCCATTGTTTCCTTGCTCCGAGAAACATACTTTTACAATGGTGGTTGAGCAAGGGAAGACCTATCTCCTTCGAATCATCAACGCCGCTCTCAACGACGAACTTTTCTTCGGCATTGCTGGACACAACCTGACAGTGGTGGAGGTTGATGCAGTTTATACCAAACCCTTCACTACTCAAGCCGTACTAATTGCACCAGGCCAGACCACAAACGTTTTGGTCAAAGCCAATCAAGCTTCAGGCAGATACTTCATGGCTGCTAGGCCTTTCATGGATGCTCCAGTTCCCGTAGACAACAAGATAGCCACTGGAATTCTCCAATACAGAGGAATTCCGAACACCGTCCTGCCAAGCCTTCCTCAATTGCCTAACCCTAACGACACGTCCTTCGCTTTAAGATACAACAATAATCTCCGGAGCCTAAACGCTCCAAACTTTCCGGCCAATGTGCCTCTCAAAGTCGACCGGAAACTCTTCTACACCATTGGTTTCGGGAAAGAATCATGCCCTAGTTGCTTCAACGGAACAAGATTTGTTGCTTCCTTGAACAACATCTCTTTCGAGATGCCGCAGGTGGGGCTTCTGCAAGCACATTACTTTAATCTCAAAGGGGTGTTCAAAACCGACTTCCCCGATAGGCCATCAACTCCTTTCAACTACACTGGTGCACCACTCACCTCCAGTCTTGGCACATCAACTGGAACTAGGCTGAGCAAGATTGCTTTCAATTCGACAGTTGAGCTGGTTTTACAAGACACGAATCTCCTAACCGTGGAATCGCATCCGTTCCATCTCCACGGATACAACTTCTTTGTTGTGGGAACTGGGATTGGGAATTTTGATCCTGCCAAGGACCCTGCTAAGTATAACTTGGTTGATCCTGTTGAGAGAAATACAGTTGGAGTCCCCACAGGTGGTTGGACTGCCATTCGGTTTAGAGCTGATAATCCAGGCGTTTGGTTCATGCACTGTCATTTGGAGCTTCATACTGGCTGGGGACTGAAAACAGCATTCGTCGTAGAAGATGGACCAGGATCGGATCAATCTGTTCTGCCTCCGCCTAAGGATCTTCCGCCTTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

566

Amino Acids

62.49

Weight (kDa)

8.86

Isoelectric Point (pI)

34.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase_3 PF07732 40 - 154 1.8e-43 Multicopper oxidase
Cu-oxidase PF00394 167 - 315 1.1e-43 Multicopper oxidase
Cu-oxidase_2 PF07731 415 - 547 4.4e-42 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000659)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G03260
fragaria_vesca FvH4_1g13060 FvH4_2g08670 FvH4_2g08670 FvH4_2g08670 FvH4_2g08840 FvH4_2g08860 FvH4_2g09061
malus_domestica MD02G1145100.v1.1 MD05G1064600.v1.1 MD10G1073700.v1.1 MD15G1258600.v1.1 MD15G1259300.v1.1
prunus_persica Prupe.6G177700_v2.0.a1 Prupe.7G156500_v2.0.a1 Prupe.8G095000_v2.0.a1 Prupe.8G095400_v2.0.a1 Prupe.8G097000_v2.0.a1
pyrus_communis pycom02g11490 pycom05g05550 pycom15g22710
rosa_chinensis RchiOBHm_Chr2g0101231 RchiOBHm_Chr6g0264061 RchiOBHm_Chr6g0264101 RchiOBHm_Chr6g0264311 RchiOBHm_Chr6g0264361 RchiOBHm_Chr6g0264441 RchiOBHm_Chr6g0264891
rosa_laevigata RLG00000014229 RLG00000014253 RLG00000014265 RLG00000014267 RLG00000014269 RLG00000014291 RLG00000014292 RLG00000014316 RLG00000017063
rosa_multiflora Rmu_co8391921.1_g000001 Rmu_co8519839.1_g000001 Rmu_sc0000546.1_g000055 Rmu_sc0002305.1_g000003 Rmu_sc0004470.1_g000004 Rmu_sc0006314.1_g000014 Rmu_sc0009807.1_g000001 Rmu_sc0010483.1_g000004
rosa_roxburghii Rroxscaffold_175G00432050 Rroxscaffold_176G00431400 Rroxscaffold_176G00431740 Rroxscaffold_176G00431790 Rroxscaffold_7G00203140 Rroxscaffold_7G00203620
rosa_rugosa Rorug02G0100200 Rorug06G0007600 Rorug06G0012500 Rorug06G0012600 Rorug06G0014100
rosa_samantha Rh2BG150700 Rh2DG150800 Rh6AG128900 Rh6AG131700 Rh6BG124500 Rh6BG124800 Rh6BG128400 Rh6BG129400 Rh6BG132600 Rh6CG123100 Rh6DG110500 Rh6DG113900 Rh6DG114900 Rh6DG118200
rosa_wichuraiana Rw2G011330 Rw6G011140 Rw6G011160 Rw6G011480 Rw6G011540 Rw6G011680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1177
Acc36I ACCTGC 1 cut(s) 1177
AccBSI CCGCTC 1 cut(s) 704
AccI GTMKAC 2 cut(s) 906, 1077
AccIII TCCGGA 2 cut(s) 18, 1029
AciI CCGC 5 cut(s) 57, 702, 1184, 1673, 1688
AclI AACGTT 1 cut(s) 828
AclWI GGATC 5 cut(s) 1451, 1487, 1657, 1662, 1689
AcoI YGGCCR 1 cut(s) 1056
AcsI RAATTY 3 cut(s) 927, 945, 1450
AcuI CTGAAG 2 cut(s) 108, 837
AfaI GTAC 2 cut(s) 287, 801
AfiI CCNNNNNNNGG 5 cut(s) 461, 751, 1190, 1387, 1529
AflIII ACRYGT 2 cut(s) 255, 996
AgsI TTSAA 9 cut(s) 122, 224, 280, 367, 1135, 1162, 1234, 1270, 1342
AhdI GACNNNNNGTC 1 cut(s) 327
AjiI CACGTC 2 cut(s) 535, 999
AjnI CCWGG 6 cut(s) 184, 564, 613, 811, 1564, 1645
AluBI AGCT 8 cut(s) 12, 86, 92, 351, 851, 1357, 1556, 1597
AluI AGCT 8 cut(s) 12, 86, 92, 351, 851, 1357, 1556, 1597
Alw21I GWGCWC 1 cut(s) 1285
Alw44I GTGCAC 1 cut(s) 1281
AlwI GGATC 5 cut(s) 1451, 1487, 1657, 1662, 1689
Aor13HI TCCGGA 2 cut(s) 18, 1029
AoxI GGCC 6 cut(s) 188, 617, 814, 878, 1056, 1253
ApaLI GTGCAC 1 cut(s) 1281
ApeKI GCWGC 4 cut(s) 9, 12, 92, 872
ApoI RAATTY 3 cut(s) 927, 945, 1450
ArsI GACNNNNNNTTYG 2 cut(s) 986, 1018
Asp700I GAANNNNTTC 1 cut(s) 1088
AspS9I GGNCC 5 cut(s) 188, 308, 617, 1468, 1643
AsuC2I CCSGG 1 cut(s) 206
AsuHPI GGTGA 2 cut(s) 224, 1282
AsuII TTCGAA 1 cut(s) 688
AsuNHI GCTAGC 1 cut(s) 5
AvaII GGWCC 3 cut(s) 308, 1468, 1643
AxyI CCTNAGG 1 cut(s) 1677
BaeGI GKGCMC 1 cut(s) 1285
BbsI GAAGAC 1 cut(s) 680
Bbv12I GWGCWC 1 cut(s) 1285
BbvI GCAGC 3 cut(s) 21, 79, 859
BccI CCATC 5 cut(s) 299, 608, 1264, 1413, 1634
BceAI ACGGC 2 cut(s) 472, 782
BciT130I CCWGG 6 cut(s) 186, 566, 615, 813, 1566, 1647
BciVI GTATCC 1 cut(s) 1409
BcnI CCSGG 1 cut(s) 206
BfaI CTAG 4 cut(s) 6, 876, 1125, 1320
BfuAI ACCTGC 1 cut(s) 1177
BfuI GTATCC 1 cut(s) 1409
BisI GCNGC 6 cut(s) 10, 13, 93, 702, 873, 1184
BlpI GCTNAGC 1 cut(s) 1325
BlsI GCNGC 6 cut(s) 11, 14, 94, 703, 874, 1185
Bme1390I CCNGG 7 cut(s) 186, 206, 566, 615, 813, 1566, 1647
Bme18I GGWCC 3 cut(s) 308, 1468, 1643
BmeRI GACNNNNNGTC 1 cut(s) 327
BmgBI CACGTC 2 cut(s) 535, 999
BmgT120I GGNCC 5 cut(s) 188, 308, 617, 1468, 1643
BmiI GGNNCC 2 cut(s) 1034, 1470
BmrFI CCNGG 7 cut(s) 186, 206, 566, 615, 813, 1566, 1647
BmrI ACTGGG 1 cut(s) 1449
BmsI GCATC 5 cut(s) 580, 754, 880, 1170, 1405
BmtI GCTAGC 1 cut(s) 9
BmuI ACTGGG 1 cut(s) 1449
BpiI GAAGAC 1 cut(s) 680
BpmI CTGGAG 3 cut(s) 66, 879, 1279
Bpu10I CCTNAGC 1 cut(s) 419
Bpu1102I GCTNAGC 1 cut(s) 1325
Bpu14I TTCGAA 1 cut(s) 688
BpuEI CTTGAG 1 cut(s) 777
BpuMI CCSGG 1 cut(s) 206
BsaJI CCNNGG 6 cut(s) 185, 565, 614, 1386, 1411, 1464
BsaWI WCCGGW 4 cut(s) 18, 341, 1029, 1080
BsaXI ACNNNNNCTCC 2 cut(s) 429, 459
Bsc4I CCNNNNNNNGG 5 cut(s) 461, 751, 1190, 1387, 1529
Bse118I RCCGGY 1 cut(s) 310
Bse1I ACTGG 9 cut(s) 109, 272, 896, 928, 1282, 1296, 1318, 1444, 1609
Bse21I CCTNAGG 1 cut(s) 1677
Bse3DI GCAATG 2 cut(s) 332, 729
BseAI TCCGGA 2 cut(s) 18, 1029
BseBI CCWGG 6 cut(s) 186, 566, 615, 813, 1566, 1647
BseDI CCNNGG 6 cut(s) 185, 565, 614, 1386, 1411, 1464
BseGI GGATG 2 cut(s) 895, 1396
BseLI CCNNNNNNNGG 5 cut(s) 461, 751, 1190, 1387, 1529
BseMI GCAATG 2 cut(s) 332, 729
BseMII CTCAG 3 cut(s) 492, 600, 1316
BseNI ACTGG 9 cut(s) 109, 272, 896, 928, 1282, 1296, 1318, 1444, 1609
BseRI GAGGAG 1 cut(s) 574
BseSI GKGCMC 1 cut(s) 1285
BseXI GCAGC 3 cut(s) 21, 79, 859
BseYI CCCAGC 1 cut(s) 1608
Bsh1285I CGRYCG 1 cut(s) 1081
BshFI GGCC 6 cut(s) 190, 619, 816, 880, 1058, 1255
BsiEI CGRYCG 1 cut(s) 1081
BsiHKAI GWGCWC 1 cut(s) 1285
BsiSI CCGG 7 cut(s) 19, 205, 311, 342, 1030, 1055, 1081
BslFI GGGAC 2 cut(s) 1505, 1626
BslI CCNNNNNNNGG 5 cut(s) 461, 751, 1190, 1387, 1529
BsmFI GGGAC 2 cut(s) 1505, 1626
BsmI GAATGC 1 cut(s) 1625
BsnI GGCC 6 cut(s) 190, 619, 816, 880, 1058, 1255
Bsp119I TTCGAA 1 cut(s) 688
Bsp1286I GDGCHC 1 cut(s) 1285
Bsp13I TCCGGA 2 cut(s) 18, 1029
Bsp143I GATC 7 cut(s) 195, 447, 1456, 1492, 1649, 1654, 1681
Bsp1720I GCTNAGC 1 cut(s) 1325
BspACI CCGC 5 cut(s) 57, 702, 1184, 1673, 1688
BspANI GGCC 6 cut(s) 190, 619, 816, 880, 1058, 1255
BspCNI CTCAG 3 cut(s) 493, 599, 1317
BspEI TCCGGA 2 cut(s) 18, 1029
BspLI GGNNCC 2 cut(s) 1034, 1470
BspMI ACCTGC 1 cut(s) 1177
BspOI GCTAGC 1 cut(s) 9
BspPI GGATC 5 cut(s) 1451, 1487, 1657, 1662, 1689
BspT104I TTCGAA 1 cut(s) 688
BsrBI CCGCTC 1 cut(s) 704
BsrDI GCAATG 2 cut(s) 332, 729
BsrFI RCCGGY 1 cut(s) 310
BsrI ACTGG 9 cut(s) 109, 272, 896, 928, 1282, 1296, 1318, 1444, 1609
BssAI RCCGGY 1 cut(s) 310
BssECI CCNNGG 6 cut(s) 185, 565, 614, 1386, 1411, 1464
BssMI GATC 7 cut(s) 195, 447, 1456, 1492, 1649, 1654, 1681
BssT1I CCWWGG 1 cut(s) 1464
Bst2UI CCWGG 6 cut(s) 186, 566, 615, 813, 1566, 1647
Bst4CI ACNGT 8 cut(s) 169, 430, 751, 958, 1351, 1387, 1513, 1586
Bst6I CTCTTC 1 cut(s) 1094
BstAPI GCANNNNNTGC 1 cut(s) 144
BstBI TTCGAA 1 cut(s) 688
BstC8I GCNNGC 4 cut(s) 7, 402, 611, 1203
BstDEI CTNAG 6 cut(s) 419, 501, 586, 1325, 1476, 1677
BstDSI CCRYGG 2 cut(s) 1386, 1411
BstENI CCTNNNNNAGG 1 cut(s) 459
BstF5I GGATG 2 cut(s) 895, 1396
BstKTI GATC 7 cut(s) 198, 450, 1459, 1495, 1652, 1657, 1684
BstMBI GATC 7 cut(s) 195, 447, 1456, 1492, 1649, 1654, 1681
BstMCI CGRYCG 1 cut(s) 1081
BstMWI GCNNNNNNNGC 5 cut(s) 92, 144, 848, 1128, 1192
BstNI CCWGG 6 cut(s) 186, 566, 615, 813, 1566, 1647
BstNSI RCATGY 2 cut(s) 259, 404
BstSCI CCNGG 7 cut(s) 184, 204, 564, 613, 811, 1564, 1645
BstSLI GKGCMC 1 cut(s) 1285
BstV1I GCAGC 3 cut(s) 21, 79, 859
BstV2I GAAGAC 1 cut(s) 680
BstX2I RGATCY 1 cut(s) 1681
BstXI CCANNNNNNTGG 1 cut(s) 1572
BstYI RGATCY 1 cut(s) 1681
Bsu36I CCTNAGG 1 cut(s) 1677
BsuI GTATCC 1 cut(s) 1409
BsuRI GGCC 6 cut(s) 190, 619, 816, 880, 1058, 1255
BtgI CCRYGG 2 cut(s) 1386, 1411
BtrI CACGTC 2 cut(s) 535, 999
BtsCI GGATG 2 cut(s) 895, 1396
BtsIMutI CAGTG 5 cut(s) 265, 756, 921, 1275, 1582
BveI ACCTGC 1 cut(s) 1177
Cac8I GCNNGC 4 cut(s) 7, 402, 611, 1203
Cfr10I RCCGGY 1 cut(s) 310
Cfr13I GGNCC 5 cut(s) 188, 308, 617, 1468, 1643
CseI GACGC 1 cut(s) 331
Csp6I GTAC 2 cut(s) 286, 800
CviAII CATG 9 cut(s) 149, 242, 256, 272, 401, 868, 886, 1119, 1579
CviQI GTAC 2 cut(s) 286, 800
DdeI CTNAG 6 cut(s) 419, 501, 586, 1325, 1476, 1677
DpnI GATC 7 cut(s) 197, 449, 1458, 1494, 1651, 1656, 1683
DpnII GATC 7 cut(s) 195, 447, 1456, 1492, 1649, 1654, 1681
DriI GACNNNNNGTC 1 cut(s) 327
EaeI YGGCCR 1 cut(s) 1056
Eam1104I CTCTTC 1 cut(s) 1094
Eam1105I GACNNNNNGTC 1 cut(s) 327
EarI CTCTTC 1 cut(s) 1094
EciI GGCGGA 2 cut(s) 1662, 1677
Eco130I CCWWGG 1 cut(s) 1464
Eco147I AGGCCT 1 cut(s) 880
Eco47I GGWCC 3 cut(s) 308, 1468, 1643
Eco57I CTGAAG 2 cut(s) 108, 837
Eco81I CCTNAGG 1 cut(s) 1677
EcoNI CCTNNNNNAGG 1 cut(s) 459
EcoO109I RGGNCCY 2 cut(s) 188, 1468
EcoRI GAATTC 2 cut(s) 927, 945
EcoRII CCWGG 6 cut(s) 184, 564, 613, 811, 1564, 1645
EcoT14I CCWWGG 1 cut(s) 1464
ErhI CCWWGG 1 cut(s) 1464
FaeI CATG 9 cut(s) 152, 245, 259, 275, 404, 871, 889, 1122, 1582
FalI AAGNNNNNCTT 4 cut(s) 373, 405, 1469, 1501
FaqI GGGAC 2 cut(s) 1505, 1626
FatI CATG 9 cut(s) 148, 241, 255, 271, 400, 867, 885, 1118, 1578
FblI GTMKAC 2 cut(s) 906, 1077
Fnu4HI GCNGC 6 cut(s) 10, 13, 93, 702, 873, 1184
FokI GGATG 2 cut(s) 902, 1383
Fsp4HI GCNGC 6 cut(s) 10, 13, 93, 702, 873, 1184
FspBI CTAG 4 cut(s) 6, 876, 1125, 1320
GluI GCNGC 6 cut(s) 10, 13, 93, 702, 873, 1184
GsaI CCCAGC 1 cut(s) 1612
GsuI CTGGAG 3 cut(s) 66, 879, 1279
HaeIII GGCC 6 cut(s) 190, 619, 816, 880, 1058, 1255
HapII CCGG 7 cut(s) 19, 205, 311, 342, 1030, 1055, 1081
HgaI GACGC 1 cut(s) 331
Hin1II CATG 9 cut(s) 152, 245, 259, 275, 404, 871, 889, 1122, 1582
HincII GTYRAC 2 cut(s) 550, 1078
HindII GTYRAC 2 cut(s) 550, 1078
HindIII AAGCTT 1 cut(s) 849
HinfI GANTC 5 cut(s) 690, 1115, 1375, 1391, 1518
HpaII CCGG 7 cut(s) 19, 205, 311, 342, 1030, 1055, 1081
HphI GGTGA 2 cut(s) 224, 1282
Hpy166II GTNNAC 5 cut(s) 532, 550, 907, 1078, 1283
Hpy188I TCNGA 4 cut(s) 589, 637, 951, 1654
Hpy188III TCNNGA 5 cut(s) 19, 337, 1030, 1108, 1177
Hpy8I GTNNAC 5 cut(s) 532, 550, 907, 1078, 1283
Hpy99I CGWCG 2 cut(s) 716, 1634
HpyAV CCTTC 6 cut(s) 171, 203, 695, 793, 980, 1012
HpyCH4III ACNGT 8 cut(s) 169, 430, 751, 958, 1351, 1387, 1513, 1586
HpyCH4IV ACGT 3 cut(s) 534, 828, 998
HpyCH4V TGCA 7 cut(s) 152, 404, 767, 809, 1201, 1283, 1582
HpyF10VI GCNNNNNNNGC 5 cut(s) 92, 144, 848, 1128, 1192
HpyF3I CTNAG 6 cut(s) 419, 501, 586, 1325, 1476, 1677
HpySE526I ACGT 3 cut(s) 534, 828, 998
Hsp92II CATG 9 cut(s) 152, 245, 259, 275, 404, 871, 889, 1122, 1582
Kpn2I TCCGGA 2 cut(s) 18, 1029
Kzo9I GATC 7 cut(s) 195, 447, 1456, 1492, 1649, 1654, 1681
LmnI GCTCC 6 cut(s) 437, 638, 898, 1032, 1048, 1594
Lsp1109I GCAGC 3 cut(s) 21, 79, 859
LweI GCATC 5 cut(s) 580, 754, 880, 1170, 1405
MaeI CTAG 4 cut(s) 6, 876, 1125, 1320
MaeII ACGT 3 cut(s) 534, 828, 998
MaeIII GTNAC 4 cut(s) 163, 232, 319, 370
MalI GATC 7 cut(s) 197, 449, 1458, 1494, 1651, 1656, 1683
MbiI CCGCTC 1 cut(s) 704
MboI GATC 7 cut(s) 195, 447, 1456, 1492, 1649, 1654, 1681
MboII GAAGA 5 cut(s) 685, 715, 1081, 1649, 1676
MfeI CAATTG 2 cut(s) 441, 977
MflI RGATCY 1 cut(s) 1681
MhlI GDGCHC 1 cut(s) 1285
MluCI AATT 8 cut(s) 261, 441, 804, 927, 945, 977, 1342, 1450
MlyI GAGTC 1 cut(s) 1527
MmeI TCCRAC 2 cut(s) 1495, 1514
MnlI CCTC 9 cut(s) 26, 377, 595, 751, 935, 984, 1077, 1303, 1680
MroI TCCGGA 2 cut(s) 18, 1029
MroXI GAANNNNTTC 1 cut(s) 1088
MseI TTAA 3 cut(s) 71, 1010, 1215
MslI CAYNNNNRTG 1 cut(s) 270
MspA1I CMGCKG 2 cut(s) 12, 86
MspI CCGG 7 cut(s) 19, 205, 311, 342, 1030, 1055, 1081
MspR9I CCNGG 7 cut(s) 186, 206, 566, 615, 813, 1566, 1647
MunI CAATTG 2 cut(s) 441, 977
Mva1269I GAATGC 1 cut(s) 1625
MvaI CCWGG 6 cut(s) 186, 566, 615, 813, 1566, 1647
MwoI GCNNNNNNNGC 5 cut(s) 92, 144, 848, 1128, 1192
NciI CCSGG 1 cut(s) 206
NdeII GATC 7 cut(s) 195, 447, 1456, 1492, 1649, 1654, 1681
NheI GCTAGC 1 cut(s) 5
NlaIII CATG 9 cut(s) 152, 245, 259, 275, 404, 871, 889, 1122, 1582
NlaIV GGNNCC 2 cut(s) 1034, 1470
NmuCI GTSAC 2 cut(s) 319, 370
NspI RCATGY 2 cut(s) 259, 404
NspV TTCGAA 1 cut(s) 688
PaeI GCATGC 1 cut(s) 404
PaqCI CACCTGC 1 cut(s) 1177
PceI AGGCCT 1 cut(s) 880
PciI ACATGT 1 cut(s) 255
PctI GAATGC 1 cut(s) 1625
PdmI GAANNNNTTC 1 cut(s) 1088
PfeI GAWTC 4 cut(s) 690, 1115, 1375, 1391
PfoI TCCNGGA 1 cut(s) 204
PkrI GCNGC 6 cut(s) 11, 14, 94, 703, 874, 1185
PleI GAGTC 1 cut(s) 1526
PpsI GAGTC 1 cut(s) 1526
PpuMI RGGWCCY 1 cut(s) 1468
PscI ACATGT 1 cut(s) 255
Psp1406I AACGTT 1 cut(s) 828
Psp5II RGGWCCY 1 cut(s) 1468
Psp6I CCWGG 6 cut(s) 184, 564, 613, 811, 1564, 1645
PspFI CCCAGC 1 cut(s) 1608
PspGI CCWGG 6 cut(s) 184, 564, 613, 811, 1564, 1645
PspN4I GGNNCC 2 cut(s) 1034, 1470
PspPI GGNCC 5 cut(s) 188, 308, 617, 1468, 1643
PspPPI RGGWCCY 1 cut(s) 1468
PsuI RGATCY 1 cut(s) 1681
PvuII CAGCTG 2 cut(s) 12, 86
RsaI GTAC 2 cut(s) 287, 801
RsaNI GTAC 2 cut(s) 286, 800
RseI CAYNNNNRTG 1 cut(s) 270
SalI GTCGAC 1 cut(s) 1076
SaqAI TTAA 3 cut(s) 71, 1010, 1215
SatI GCNGC 6 cut(s) 10, 13, 93, 702, 873, 1184
Sau3AI GATC 7 cut(s) 195, 447, 1456, 1492, 1649, 1654, 1681
Sau96I GGNCC 5 cut(s) 188, 308, 617, 1468, 1643
SchI GAGTC 1 cut(s) 1527
ScrFI CCNGG 7 cut(s) 186, 206, 566, 615, 813, 1566, 1647
SduI GDGCHC 1 cut(s) 1285
SfaNI GCATC 5 cut(s) 580, 754, 880, 1170, 1405
SfuI TTCGAA 1 cut(s) 688
SinI GGWCC 3 cut(s) 308, 1468, 1643
SmiMI CAYNNNNRTG 1 cut(s) 270
SmlI CTYRAG 1 cut(s) 792
SmoI CTYRAG 1 cut(s) 792
SphI GCATGC 1 cut(s) 404
Sse9I AATT 8 cut(s) 261, 441, 804, 927, 945, 977, 1342, 1450
SseBI AGGCCT 1 cut(s) 880
SsiI CCGC 5 cut(s) 57, 702, 1184, 1673, 1688
SspMI CTAG 4 cut(s) 6, 876, 1125, 1320
StuI AGGCCT 1 cut(s) 880
StyD4I CCNGG 7 cut(s) 184, 204, 564, 613, 811, 1564, 1645
StyI CCWWGG 1 cut(s) 1464
TaaI ACNGT 8 cut(s) 169, 430, 751, 958, 1351, 1387, 1513, 1586
TaiI ACGT 3 cut(s) 537, 831, 1001
TaqI TCGA 4 cut(s) 688, 1077, 1176, 1346
TasI AATT 8 cut(s) 261, 441, 804, 927, 945, 977, 1342, 1450
TauI GCSGC 2 cut(s) 704, 1186
TfiI GAWTC 4 cut(s) 690, 1115, 1375, 1391
Tru1I TTAA 3 cut(s) 71, 1010, 1215
Tru9I TTAA 3 cut(s) 71, 1010, 1215
TscAI CASTG 5 cut(s) 272, 756, 928, 1282, 1589
TseFI GTSAC 2 cut(s) 319, 370
TseI GCWGC 4 cut(s) 9, 12, 92, 872
Tsp45I GTSAC 2 cut(s) 319, 370
TspDTI ATGAA 4 cut(s) 856, 874, 1567, 1589
TspGWI ACGGA 3 cut(s) 1152, 1389, 1428
TspRI CASTG 5 cut(s) 272, 756, 928, 1282, 1589
VneI GTGCAC 1 cut(s) 1281
VpaK11BI GGWCC 3 cut(s) 308, 1468, 1643
XagI CCTNNNNNAGG 1 cut(s) 459
XapI RAATTY 3 cut(s) 927, 945, 1450
XceI RCATGY 2 cut(s) 259, 404
XcmI CCANNNNNNNNNTGG 1 cut(s) 829
XmiI GTMKAC 2 cut(s) 906, 1077
XmnI GAANNNNTTC 1 cut(s) 1088
XspI CTAG 4 cut(s) 6, 876, 1125, 1320
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.