Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0001708

Physical Location & Seq

Genomic Coordinates Reverse (-)

25597121 .. 25601600

Gene Structure 4480 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1187200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2208 bp

ATGTTCAATAAGGTTGATCTCTCTGTTTCCTCGTACGATACTGCTTGGGTGGCAATGGTCCCTTCTCCAAATTCTCAGGACCCTTTTTTCCCTGAATGTGTCAACTGGCTATTGTGTAATCAACTCCACGACGGGTCATGGGGTCATCCTAATTTCCATCCTTTGTTAAGGAAAGATGCTCTTTTATCTACGTTAGCATGTATCCTTGCACTAAAGCAATGGAGTGTTGGTGAAGAGCTAATTAACAACGGACTACGTTTTATTGAGTCAAATATATCTTCAGCTACCGATGAAGTGCAACAATCTCCTCTTGGATTTGATATAATATTTCCTTCTTTGATACAATCCGCAATGAATTTGGATATCAATCTTCCCCTTGAAGAATCAACCCTGGCCAGTATTTTCCACATGAGAGACTTTGAGCTTCAAAGAGGAAATGGAAGCAACTCAGAGGGTTGGAGAGCCTTCTTGGCTTATATTTCGGAAGGAAATGCAGAGTTGCAGGATTGGCAGACGGTTATGAAGTACCAAAGGAAGAATGGGTCCTTGTTTAATTCACCATCAACCACAGCCGCTGCTTTTACTCACACTAAGAATGCTGATTGTCTTCAGTACCTCCGCAGACTCTTAGACAAGTTTGGGAATGCAGTTCCAACGATATATCCTCTAGATAAATATGCTCGTCTTTCTATGGTTTCCAGCCTTGAAAGTTTGGGTATCGATCGACATTTCAGGGAGGAAATTAGAAGCGTAATCGATGAAACATACAGATTATGGCTGCAGGGGGATGAAGAAATATTTTCAGATGCTGCCACCTGTGCAATGGCGTTTCGACTCTTACGCGTTAATGGATATGACATTTCTGCAGATCCATTAATAAAATTTTCCGAAGATTGTTTCTTCAATTCCTACGGAGGATACTTGAAGGACGTTAGTGCTGCCTTAGAATTGTTTAGGGCTTCCGAAATCATCATACATCCAGATGAGTCAATTCTTGAGAAACAAAACTACTGGACAAGTCATTTTCTGAAAGAGGAGTTATCCAATATGTTAATTCAGCCTCATAGACCGAATAAGCACATTGCCCTGGAGGTGGATCATGCTCTTAAATCCCCTTACCATGCAAATTTGGGTCGTTTATCAACCAAGAGAGCTATAAAACATTACAACACAGATGGTACAAGGATTCTGAAATCTTCTTATAGCTCTTTGAATATTGGACATGAAGATTTCCTCGAACTGGCAGTGGAAGACTATAACATTTGCCAATCTATACATCGTAAAGAATTCAACCATCTTGAAAGGTGGGTTGTTGAGCACAGATTAGACAAGCTAAAGTTTGCTCGGCAGAAGCAAGCGTACTGCTACCTCTCTGCTGCAGCAGACCTTTTCCCTCCGGAACTATCAGATGCCCGCATGTCATGGGCCAAAAATGCATTTCTCACAACTGTGGTTGATGATTTCTTTGACATTGATGGTTCGGAAGAGGAACTAATAAACCTTATACAATTGGTCGAGAAGTGGGACTTAGATGCAAGTGTTGATTGTTGTTCTAAGCACGTTGAAATCATATTTTCAGCACTTAGGGACACAATTAGTGAGATTGGAGTCAAAGCGTTCAACTTGCAAGGACGTAATGTAACAACTGACGTAATTAAGATTTGGTTGGATTGCCTGAACTCTATGTTAAAAGAAGCTGAGTGGTCGAGAAACAAGTCCGTGCCAACAATGGATGAATATATGGCAAATGCAGGAACATCATTTGCCTTGGGACCAATTGTCCTCCCAGCTCTCTATTTTGTTGGGCCTAAACTTTCAGAGGAGGTTGTAGGAAATTCTGAACTCCAGTATCTGTTCACGCTTATGAGCACTTGTGGGCGTCTTCTCAACGATGTCCAAAGCTTTAAGAGGGAATTAGCGGAAGGGAAGTTAAATGCTGTATCGTTGGCCACGATTCATGGTGGTGGTAATGTATCTGAGGAAGATGCGATCAATGATATTAAGAACGTTATAACAAGTGAGACGAGAGAACTGCTAAGAGTTGTTTTGCTGGAGAAGGGTAGCGCAGTTCCAAGAGCTTGCAAGGATTTGTTTTGGAACTTGAACAAAGTGATGCACCTTTTTTATTGGAAGGATGATGGGTATGTTGGGAACGAGATGATGAAGGCCGTAAATGAAGTCATTGGAGAACTTATTGTTCTTCAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006629 GO:0006720 GO:0006721 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009685 GO:0009686 GO:0009719 GO:0009725 GO:0009739 GO:0009740 GO:0009755 GO:0009899 GO:0009987 GO:0010033 GO:0010333 GO:0010476 GO:0010817 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016829 GO:0016835 GO:0016838 GO:0019752 GO:0023052 GO:0032870 GO:0033993 GO:0042221 GO:0042445 GO:0042446 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0065008 GO:0070887 GO:0071229 GO:0071310 GO:0071370 GO:0071396 GO:0071495 GO:0071704 GO:1901576 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko00904 ko01100 ko01110 map00904 map01100 map01110

Pfam Domains

Protein Families

Terpene_synth Terpene_synth_C

Protein Analysis

736

Amino Acids

83.4

Weight (kDa)

5.14

Isoelectric Point (pI)

45.24

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Terpene_synth	PF01397	169 - 369	5.5e-42	Terpene synthase, N-terminal domain
Terpene_synth_C	PF03936	441 - 676	5.4e-63	Terpene synthase family, metal binding domain
Terpene_syn_C_2	PF19086	480 - 676	2.5e-40	Terpene synthase family 2, C-terminal metal binding

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001708)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G79460
fragaria_vesca	FvH4_3g14116 FvH4_3g14130 FvH4_3g14131 FvH4_3g16270
malus_domestica	MD03G1187200.v1.1 MD10G1215700.v1.1 MD10G1215900.v1.1 MD10G1216200.v1.1
prunus_persica	Prupe.4G128500_v2.0.a1 Prupe.4G128500_v2.0.a1 Prupe.4G128500_v2.0.a1 Prupe.4G128600_v2.0.a1 Prupe.4G128700_v2.0.a1
pyrus_communis	pycom03g13990 pycom10g18220
rosa_chinensis	RchiOBHm_Chr5g0023441 RchiOBHm_Chr5g0023471 RchiOBHm_Chr5g0023641
rosa_laevigata	RLG00000032721
rosa_multiflora	Rmu_co8406553.1_g000001 Rmu_sc0004593.1_g000004 Rmu_sc0005664.1_g000001 Rmu_sc0005664.1_g000005
rosa_roxburghii	Rroxscaffold_1G00055600 Rroxscaffold_1G00055620 Rroxscaffold_1G00055910
rosa_rugosa	Rorug05G0079200 Rorug05G0079300
rosa_samantha	Rh5AG166100 Rh5AG166200 Rh5AG166300 Rh5AG167600 Rh5AG168000 Rh5BG165300 Rh5BG165500 Rh5CG180300 Rh5CG180400 Rh5CG181400 Rh5DG166800 Rh5DG166900 Rh5DG167800
rosa_wichuraiana	Rw0G019600 Rw5G014950 Rw5G014970 Rw5G015110 Rw5G015120 Rw5G015210

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2012
AccII	CGCG	1 cut(s)	843
AccIII	TCCGGA	1 cut(s)	1396
AciI	CCGC	5 cut(s)	348, 573, 619, 1414, 1919
AclI	AACGTT	1 cut(s)	2007
AclWI	GGATC	2 cut(s)	863, 1104
AcoI	YGGCCR	2 cut(s)	393, 1947
AcsI	RAATTY	6 cut(s)	70, 355, 881, 1126, 1286, 1834
AcuI	CTGAAG	2 cut(s)	264, 593
AcyI	GRCGYC	1 cut(s)	1879
AfaI	GTAC	5 cut(s)	35, 527, 614, 1180, 1361
AfiI	CCNNNNNNNGG	2 cut(s)	1093, 1240
AflIII	ACRYGT	1 cut(s)	841
AhdI	GACNNNNNGTC	1 cut(s)	1778
AjnI	CCWGG	2 cut(s)	390, 1086
AleI	CACNNNNGTG	1 cut(s)	1448
Alw21I	GWGCWC	2 cut(s)	1320, 1871
Alw26I	GTCTC	2 cut(s)	408, 2015
AlwI	GGATC	2 cut(s)	863, 1104
AlwNI	CAGNNNCTG	3 cut(s)	575, 809, 1852
Aor13HI	TCCGGA	1 cut(s)	1396
AoxI	GGCC	5 cut(s)	393, 1425, 1805, 1947, 2166
ApeKI	GCWGC	6 cut(s)	575, 778, 809, 938, 1376, 1379
ApoI	RAATTY	6 cut(s)	70, 355, 881, 1126, 1286, 1834
AseI	ATTAAT	1 cut(s)	875
AspLEI	GCGC	1 cut(s)	2066
AspS9I	GGNCC	6 cut(s)	58, 79, 543, 1425, 1772, 1805
AsuHPI	GGTGA	2 cut(s)	242, 549
AvaII	GGWCC	4 cut(s)	58, 79, 543, 1772
BaeI	ACNNNNGTAYC	2 cut(s)	1832, 1865
BalI	TGGCCA	2 cut(s)	395, 1949
BarI	GAAGNNNNNNTAC	2 cut(s)	1343, 1375
BbsI	GAAGAC	3 cut(s)	599, 1257, 1874
Bbv12I	GWGCWC	2 cut(s)	1320, 1871
BbvI	GCAGC	6 cut(s)	562, 765, 796, 925, 1363, 1391
BccI	CCATC	6 cut(s)	165, 568, 1169, 1302, 1469, 2132
BceAI	ACGGC	1 cut(s)	2153
BcgI	CGANNNNNNTGC	2 cut(s)	2014, 2048
BciT130I	CCWGG	2 cut(s)	392, 1088
BciVI	GTATCC	2 cut(s)	212, 911
BcoDI	GTCTC	2 cut(s)	408, 2015
BfaI	CTAG	1 cut(s)	668
BfmI	CTRYAG	3 cut(s)	779, 864, 1377
BfuI	GTATCC	2 cut(s)	212, 911
BglI	GCCNNNNNGGC	1 cut(s)	470
BisI	GCNGC	7 cut(s)	573, 576, 779, 810, 939, 1377, 1380
BlsI	GCNGC	7 cut(s)	574, 577, 780, 811, 940, 1378, 1381
Bme1390I	CCNGG	2 cut(s)	392, 1088
Bme18I	GGWCC	4 cut(s)	58, 79, 543, 1772
BmeRI	GACNNNNNGTC	1 cut(s)	1778
BmgT120I	GGNCC	6 cut(s)	58, 79, 543, 1425, 1772, 1805
BmiI	GGNNCC	4 cut(s)	60, 81, 544, 1773
BmrFI	CCNGG	2 cut(s)	392, 1088
BmsI	GCATC	6 cut(s)	166, 796, 1399, 1522, 1975, 2103
BpiI	GAAGAC	3 cut(s)	599, 1257, 1874
BpmI	CTGGAG	3 cut(s)	1109, 1829, 2072
BpuEI	CTTGAG	1 cut(s)	1016
Bsa29I	ATCGAT	2 cut(s)	720, 756
BsaBI	GATNNNNATC	1 cut(s)	366
BsaHI	GRCGYC	1 cut(s)	1879
BsaJI	CCNNGG	3 cut(s)	390, 1086, 1767
BsaWI	WCCGGW	1 cut(s)	1396
BsaXI	ACNNNNNCTCC	2 cut(s)	214, 244
Bsc4I	CCNNNNNNNGG	2 cut(s)	1093, 1240
Bse1I	ACTGG	5 cut(s)	110, 396, 1016, 1245, 1846
Bse3DI	GCAATG	5 cut(s)	60, 224, 357, 828, 1080
Bse8I	GATNNNNATC	1 cut(s)	366
BseAI	TCCGGA	1 cut(s)	1396
BseBI	CCWGG	2 cut(s)	392, 1088
BseCI	ATCGAT	2 cut(s)	720, 756
BseDI	CCNNGG	3 cut(s)	390, 1086, 1767
BseGI	GGATG	6 cut(s)	145, 157, 793, 976, 1738, 2140
BseJI	GATNNNNATC	1 cut(s)	366
BseLI	CCNNNNNNNGG	2 cut(s)	1093, 1240
BseMI	GCAATG	5 cut(s)	60, 224, 357, 828, 1080
BseMII	CTCAG	4 cut(s)	89, 462, 1689, 1968
BseNI	ACTGG	5 cut(s)	110, 396, 1016, 1245, 1846
BseRI	GAGGAG	3 cut(s)	297, 1049, 1835
BseXI	GCAGC	6 cut(s)	562, 765, 796, 925, 1363, 1391
BseYI	CCCAGC	1 cut(s)	1786
Bsh1236I	CGCG	1 cut(s)	843
Bsh1285I	CGRYCG	1 cut(s)	724
BshFI	GGCC	5 cut(s)	395, 1427, 1807, 1949, 2168
BshVI	ATCGAT	2 cut(s)	720, 756
BsiEI	CGRYCG	1 cut(s)	724
BsiHKAI	GWGCWC	2 cut(s)	1320, 1871
BsiSI	CCGG	1 cut(s)	1397
BsiWI	CGTACG	1 cut(s)	33
BslFI	GGGAC	4 cut(s)	44, 1538, 1601, 1785
BslI	CCNNNNNNNGG	2 cut(s)	1093, 1240
BsmAI	GTCTC	2 cut(s)	408, 2015
BsmBI	CGTCTC	1 cut(s)	2015
BsmFI	GGGAC	4 cut(s)	44, 1538, 1601, 1785
BsmI	GAATGC	2 cut(s)	601, 649
BsnI	GGCC	5 cut(s)	395, 1427, 1807, 1949, 2168
Bsp1286I	GDGCHC	2 cut(s)	1320, 1871
Bsp13I	TCCGGA	1 cut(s)	1396
Bsp143I	GATC	5 cut(s)	16, 721, 868, 1096, 1989
BspACI	CCGC	5 cut(s)	348, 573, 619, 1414, 1919
BspANI	GGCC	5 cut(s)	395, 1427, 1807, 1949, 2168
BspCNI	CTCAG	4 cut(s)	88, 461, 1690, 1969
BspDI	ATCGAT	2 cut(s)	720, 756
BspEI	TCCGGA	1 cut(s)	1396
BspFNI	CGCG	1 cut(s)	843
BspLI	GGNNCC	4 cut(s)	60, 81, 544, 1773
BspMAI	CTGCAG	3 cut(s)	783, 868, 1381
BspPI	GGATC	2 cut(s)	863, 1104
BspQI	GCTCTTC	1 cut(s)	228
BsrDI	GCAATG	5 cut(s)	60, 224, 357, 828, 1080
BsrI	ACTGG	5 cut(s)	110, 396, 1016, 1245, 1846
BssECI	CCNNGG	3 cut(s)	390, 1086, 1767
BssMI	GATC	5 cut(s)	16, 721, 868, 1096, 1989
BssNI	GRCGYC	1 cut(s)	1879
BssT1I	CCWWGG	1 cut(s)	1767
Bst2UI	CCWGG	2 cut(s)	392, 1088
Bst4CI	ACNGT	2 cut(s)	517, 1450
Bst6I	CTCTTC	2 cut(s)	228, 1479
BstACI	GRCGYC	1 cut(s)	1879
BstC8I	GCNNGC	3 cut(s)	1356, 1414, 2080
BstF5I	GGATG	6 cut(s)	145, 157, 793, 976, 1738, 2140
BstFNI	CGCG	1 cut(s)	843
BstHHI	GCGC	1 cut(s)	2066
BstKTI	GATC	5 cut(s)	19, 724, 871, 1099, 1992
BstMAI	GTCTC	2 cut(s)	408, 2015
BstMBI	GATC	5 cut(s)	16, 721, 868, 1096, 1989
BstMCI	CGRYCG	1 cut(s)	724
BstMWI	GCNNNNNNNGC	5 cut(s)	50, 470, 508, 818, 1433
BstNI	CCWGG	2 cut(s)	392, 1088
BstNSI	RCATGY	2 cut(s)	201, 1420
BstSCI	CCNGG	2 cut(s)	390, 1086
BstSFI	CTRYAG	3 cut(s)	779, 864, 1377
BstUI	CGCG	1 cut(s)	843
BstV1I	GCAGC	6 cut(s)	562, 765, 796, 925, 1363, 1391
BstV2I	GAAGAC	3 cut(s)	599, 1257, 1874
BstX2I	RGATCY	1 cut(s)	868
BstYI	RGATCY	1 cut(s)	868
Bsu15I	ATCGAT	2 cut(s)	720, 756
BsuI	GTATCC	2 cut(s)	212, 911
BsuRI	GGCC	5 cut(s)	395, 1427, 1807, 1949, 2168
BsuTUI	ATCGAT	2 cut(s)	720, 756
BtsCI	GGATG	6 cut(s)	145, 157, 793, 976, 1738, 2140
BtsI	GCAGTG	1 cut(s)	1251
BtsIMutI	CAGTG	1 cut(s)	1251
Cac8I	GCNNGC	3 cut(s)	1356, 1414, 2080
CaiI	CAGNNNCTG	3 cut(s)	575, 809, 1852
CfoI	GCGC	1 cut(s)	2066
Cfr13I	GGNCC	6 cut(s)	58, 79, 543, 1425, 1772, 1805
ClaI	ATCGAT	2 cut(s)	720, 756
CseI	GACGC	1 cut(s)	1868
Csp6I	GTAC	5 cut(s)	34, 526, 613, 1179, 1360
CviAII	CATG	9 cut(s)	138, 198, 409, 1100, 1121, 1223, 1417, 1422, 1958
CviQI	GTAC	5 cut(s)	34, 526, 613, 1179, 1360
DpnI	GATC	5 cut(s)	18, 723, 870, 1098, 1991
DpnII	GATC	5 cut(s)	16, 721, 868, 1096, 1989
DriI	GACNNNNNGTC	1 cut(s)	1778
EaeI	YGGCCR	2 cut(s)	393, 1947
Eam1104I	CTCTTC	2 cut(s)	228, 1479
Eam1105I	GACNNNNNGTC	1 cut(s)	1778
EarI	CTCTTC	2 cut(s)	228, 1479
Eco130I	CCWWGG	1 cut(s)	1767
Eco32I	GATATC	1 cut(s)	364
Eco47I	GGWCC	4 cut(s)	58, 79, 543, 1772
Eco57I	CTGAAG	2 cut(s)	264, 593
EcoO109I	RGGNCCY	2 cut(s)	79, 543
EcoRI	GAATTC	1 cut(s)	1286
EcoRII	CCWGG	2 cut(s)	390, 1086
EcoRV	GATATC	1 cut(s)	364
EcoT14I	CCWWGG	1 cut(s)	1767
EcoT22I	ATGCAT	1 cut(s)	1438
ErhI	CCWWGG	1 cut(s)	1767
Esp3I	CGTCTC	1 cut(s)	2015
FaeI	CATG	9 cut(s)	141, 201, 412, 1103, 1124, 1226, 1420, 1425, 1961
FalI	AAGNNNNNCTT	4 cut(s)	165, 197, 1511, 1543
FaqI	GGGAC	4 cut(s)	44, 1538, 1601, 1785
FatI	CATG	9 cut(s)	137, 197, 408, 1099, 1120, 1222, 1416, 1421, 1957
FauI	CCCGC	1 cut(s)	1421
Fnu4HI	GCNGC	7 cut(s)	573, 576, 779, 810, 939, 1377, 1380
FokI	GGATG	6 cut(s)	132, 144, 800, 963, 1745, 2147
Fsp4HI	GCNGC	7 cut(s)	573, 576, 779, 810, 939, 1377, 1380
FspBI	CTAG	1 cut(s)	668
GlaI	GCGC	1 cut(s)	2065
GluI	GCNGC	7 cut(s)	573, 576, 779, 810, 939, 1377, 1380
GsaI	CCCAGC	1 cut(s)	1790
GsuI	CTGGAG	3 cut(s)	1109, 1829, 2072
HaeIII	GGCC	5 cut(s)	395, 1427, 1807, 1949, 2168
HapII	CCGG	1 cut(s)	1397
HgaI	GACGC	1 cut(s)	1868
HhaI	GCGC	1 cut(s)	2066
Hin1I	GRCGYC	1 cut(s)	1879
Hin1II	CATG	9 cut(s)	141, 201, 412, 1103, 1124, 1226, 1420, 1425, 1961
Hin6I	GCGC	1 cut(s)	2064
HinP1I	GCGC	1 cut(s)	2064
HincII	GTYRAC	1 cut(s)	103
HindII	GTYRAC	1 cut(s)	103
HindIII	AAGCTT	1 cut(s)	1900
HinfI	GANTC	8 cut(s)	266, 383, 624, 834, 986, 1186, 1608, 1954
HpaII	CCGG	1 cut(s)	1397
HphI	GGTGA	2 cut(s)	242, 549
Hpy166II	GTNNAC	2 cut(s)	103, 1857
Hpy188III	TCNNGA	8 cut(s)	77, 668, 980, 995, 1298, 1397, 1516, 1707
Hpy8I	GTNNAC	2 cut(s)	103, 1857
Hpy99I	CGWCG	1 cut(s)	134
HpyAV	CCTTC	9 cut(s)	72, 342, 475, 479, 919, 1916, 2050, 2125, 2158
HpyCH4III	ACNGT	2 cut(s)	517, 1450
HpyCH4IV	ACGT	7 cut(s)	191, 256, 930, 1560, 1633, 1650, 2007
HpyF10VI	GCNNNNNNNGC	5 cut(s)	50, 470, 508, 818, 1433
HpySE526I	ACGT	7 cut(s)	191, 256, 930, 1560, 1633, 1650, 2007
Hsp92I	GRCGYC	1 cut(s)	1879
Hsp92II	CATG	9 cut(s)	141, 201, 412, 1103, 1124, 1226, 1420, 1425, 1961
HspAI	GCGC	1 cut(s)	2064
Kpn2I	TCCGGA	1 cut(s)	1396
Kzo9I	GATC	5 cut(s)	16, 721, 868, 1096, 1989
LguI	GCTCTTC	1 cut(s)	228
Lsp1109I	GCAGC	6 cut(s)	562, 765, 796, 925, 1363, 1391
LweI	GCATC	6 cut(s)	166, 796, 1399, 1522, 1975, 2103
MaeI	CTAG	1 cut(s)	668
MaeII	ACGT	7 cut(s)	191, 256, 930, 1560, 1633, 1650, 2007
MaeIII	GTNAC	1 cut(s)	1639
MalI	GATC	5 cut(s)	18, 723, 870, 1098, 1991
MboI	GATC	5 cut(s)	16, 721, 868, 1096, 1989
MfeI	CAATTG	2 cut(s)	1508, 1776
MflI	RGATCY	1 cut(s)	868
MhlI	GDGCHC	2 cut(s)	1320, 1871
MlsI	TGGCCA	2 cut(s)	395, 1949
MluI	ACGCGT	1 cut(s)	841
MluNI	TGGCCA	2 cut(s)	395, 1949
MlyI	GAGTC	5 cut(s)	275, 618, 828, 995, 1617
MmeI	TCCRAC	3 cut(s)	437, 677, 1647
Mox20I	TGGCCA	2 cut(s)	395, 1949
Mph1103I	ATGCAT	1 cut(s)	1438
MroI	TCCGGA	1 cut(s)	1396
MscI	TGGCCA	2 cut(s)	395, 1949
MslI	CAYNNNNRTG	4 cut(s)	981, 1448, 1862, 1962
Msp20I	TGGCCA	2 cut(s)	395, 1949
MspA1I	CMGCKG	1 cut(s)	575
MspI	CCGG	1 cut(s)	1397
MspR9I	CCNGG	2 cut(s)	392, 1088
MunI	CAATTG	2 cut(s)	1508, 1776
Mva1269I	GAATGC	2 cut(s)	601, 649
MvaI	CCWGG	2 cut(s)	392, 1088
MvnI	CGCG	1 cut(s)	843
MwoI	GCNNNNNNNGC	5 cut(s)	50, 470, 508, 818, 1433
NdeII	GATC	5 cut(s)	16, 721, 868, 1096, 1989
NlaIII	CATG	9 cut(s)	141, 201, 412, 1103, 1124, 1226, 1420, 1425, 1961
NlaIV	GGNNCC	4 cut(s)	60, 81, 544, 1773
NmeAIII	GCCGAG	1 cut(s)	1324
NsiI	ATGCAT	1 cut(s)	1438
NspI	RCATGY	2 cut(s)	201, 1420
OliI	CACNNNNGTG	1 cut(s)	1448
PciSI	GCTCTTC	1 cut(s)	228
PcsI	WCGNNNNNNNCGW	1 cut(s)	1949
PctI	GAATGC	2 cut(s)	601, 649
PfeI	GAWTC	3 cut(s)	383, 1186, 1954
Pfl23II	CGTACG	1 cut(s)	33
PkrI	GCNGC	7 cut(s)	574, 577, 780, 811, 940, 1378, 1381
Ple19I	CGATCG	1 cut(s)	724
PleI	GAGTC	5 cut(s)	274, 618, 828, 994, 1616
PpsI	GAGTC	5 cut(s)	274, 618, 828, 994, 1616
PpuMI	RGGWCCY	2 cut(s)	79, 543
PshBI	ATTAAT	1 cut(s)	875
PsiI	TTATAA	1 cut(s)	2012
Psp1406I	AACGTT	1 cut(s)	2007
Psp5II	RGGWCCY	2 cut(s)	79, 543
Psp6I	CCWGG	2 cut(s)	390, 1086
PspFI	CCCAGC	1 cut(s)	1786
PspGI	CCWGG	2 cut(s)	390, 1086
PspLI	CGTACG	1 cut(s)	33
PspN4I	GGNNCC	4 cut(s)	60, 81, 544, 1773
PspPI	GGNCC	6 cut(s)	58, 79, 543, 1425, 1772, 1805
PspPPI	RGGWCCY	2 cut(s)	79, 543
PstI	CTGCAG	3 cut(s)	783, 868, 1381
PstNI	CAGNNNCTG	3 cut(s)	575, 809, 1852
PsuI	RGATCY	1 cut(s)	868
PvuI	CGATCG	1 cut(s)	724
RsaI	GTAC	5 cut(s)	35, 527, 614, 1180, 1361
RsaNI	GTAC	5 cut(s)	34, 526, 613, 1179, 1360
RseI	CAYNNNNRTG	4 cut(s)	981, 1448, 1862, 1962
SapI	GCTCTTC	1 cut(s)	228
SatI	GCNGC	7 cut(s)	573, 576, 779, 810, 939, 1377, 1380
Sau3AI	GATC	5 cut(s)	16, 721, 868, 1096, 1989
Sau96I	GGNCC	6 cut(s)	58, 79, 543, 1425, 1772, 1805
SchI	GAGTC	5 cut(s)	275, 618, 828, 995, 1617
ScrFI	CCNGG	2 cut(s)	392, 1088
SduI	GDGCHC	2 cut(s)	1320, 1871
SfaNI	GCATC	6 cut(s)	166, 796, 1399, 1522, 1975, 2103
SfcI	CTRYAG	3 cut(s)	779, 864, 1377
SinI	GGWCC	4 cut(s)	58, 79, 543, 1772
SmiMI	CAYNNNNRTG	4 cut(s)	981, 1448, 1862, 1962
SmlI	CTYRAG	1 cut(s)	995
SmoI	CTYRAG	1 cut(s)	995
SsiI	CCGC	5 cut(s)	348, 573, 619, 1414, 1919
SspI	AATATT	3 cut(s)	327, 798, 1216
SspMI	CTAG	1 cut(s)	668
StyD4I	CCNGG	2 cut(s)	390, 1086
StyI	CCWWGG	1 cut(s)	1767
TaaI	ACNGT	2 cut(s)	517, 1450
TaiI	ACGT	7 cut(s)	194, 259, 933, 1563, 1636, 1653, 2010
TaqI	TCGA	7 cut(s)	720, 724, 756, 832, 1236, 1515, 1706
TaqII	GACCGA	1 cut(s)	1084
TauI	GCSGC	1 cut(s)	575
TfiI	GAWTC	3 cut(s)	383, 1186, 1954
TscAI	CASTG	1 cut(s)	1251
TseI	GCWGC	6 cut(s)	575, 778, 809, 938, 1376, 1379
TspGWI	ACGGA	3 cut(s)	264, 927, 1708
TspRI	CASTG	1 cut(s)	1251
VpaK11BI	GGWCC	4 cut(s)	58, 79, 543, 1772
VspI	ATTAAT	1 cut(s)	875
XapI	RAATTY	6 cut(s)	70, 355, 881, 1126, 1286, 1834
XbaI	TCTAGA	1 cut(s)	667
XceI	RCATGY	2 cut(s)	201, 1420
XcmI	CCANNNNNNNNNTGG	2 cut(s)	536, 820
XspI	CTAG	1 cut(s)	668
Zsp2I	ATGCAT	1 cut(s)	1438

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.