Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000001

Orthogroup ID

OG0001708

Physical Location & Seq

Genomic Coordinates Reverse (-)

77144134 .. 77149630

Gene Structure 5497 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_1G00055600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2388 bp

ATGTCCTTCTCCCAGCTCAACACTTTCAGGTGTTGCACCTTTTCCCCATCAGGAAAAGATCTCTTGCCACAAGCAGCTTCAACAGTTCCAAGGACAAATGGCACAGCAGAAGTAAATACTGCTGTTTTGAGTTTGGAAGGAACTAAAAAAAGAATAAAGGAGATGTTCAACAAGGTTGATCTCTCTGTTTCATCATATGACACTGCTTGGGTGGCAATGGTCCCTTCTCCAAATTCCGGGAAGGAGCCTTTCTTCCCTGAATGCGTAAATTGGTTGTTAGATAATCAACTTCATGATGGCTCATGGGGTCCTCCTAATCTGCATCAGTTGTTAACAAAAGATGCCCTCTTATCCACGTTAGCATGCATCGTTGCACTAAAGCGATGGAATATTGGTGAAGAACTAATTGACAAGGGTCTGCATTTTATCGAGTCAAATTTAGCTTCAGCTACTGATGGAGAGCAACCATCTCCTGTTGGGTTTGATATAATATTTCCTTCCATGATTGAATCTGCAATGAATTTGGATGTGAATCTACCTTTGGGAGGATTAACCTTAGATGCTTTACTTCTCAGGAGAGACTTTGAGCTTCAAAGAGGCAACAGAAGCAACTCAGAGGGGTGGAGAGCCTATCTCGCATATATTTCAGAAGGAATCGGAAAGTCACAGGACTGGGAGATGGTCATGAAGTACCAGAAGAAGAATGGGTCCTTGTTTAACTCACCATCAACTACTGCTGCTGCTTTTATTCACCTCAACAATGCTGGTTGTCTTAGTTATCTACGCTCACTCATAGAAAAGTTTGGAAGTGCAGTTCCAACGATTTATCCTCTAGATAACTATGCTCGCCTTTCTATGGTTTCCAGTCTTGAAAGTATGGGCATTGATCGACATTTCAGGGAGGAAATAAGGAGTGTACTCGATGAAACATACAGATGCTGGCTGCAGGGGGATGAAGATATATTTTCAGATGCTGGCACCTGTGCTATGGCATTTCGACTCTTACGTCTTCATGGATATGATGTTTCTGCAGATCCATTAAGTCAGTTTTCAGAAGACAGTTTCTTCAATTCCCTTGGAGGATATCTGAAGGACACTGGTGCTGCCTTAGAACTTTTTAGGGCTTCAGAAGTCATCATTCATCCAGATGAATCAGTTCTAGAGAAACAACATTACTGGACAAGTCATTTCTTGAAACAGGAATTATCAAATAACTTAACTCAGGCTCATAAACTTAATAAGCATATTGATCAGGTGGATGATGCTCTTCGGTTTCCTTCCTATGCAACTCTGGGTCGGTTATCAAGCAGGAAAGCAATAAAATATTACTACACTGATAGTACAAAGATTTTAAAATCTTCTTATCGTTGTTCAAATGTTGGGAATGAAGACTTCCTAAAACTGGCAGTGGAAGACTTCAATATGTGTCAATTGATACATCGGGAAGAACTCGAGTATCTTTCAAGGTGGGTTGTCAAGAGCAGATTAGACAAGCTAGAATTTGCTAGGCAGAAGCAGGCATACTGTTACTTCTCTGCTGCAGCAACCCTTTTCCCTCCTGAACTATCAGATGCCCGCATATCATGGGCCAAAAACGGAGTGCTTACAACTGTGGTTGATGATTTCTTTGACGTTGGAGGTTCTGAAGAGGAACTAGTAAACCTTATACAATTGATCGAGAAGTGGGATGTGAATCCAAAAACTGATTCTTGTTCTGAGCAAGTTGAAATCATATTTTCAGCAATTAAGAGCACAATTAATGAGATTGGAGCCAATGCATTCCCAGGGCAACAGCGCAGTGTAACAAATCACGTCATAGAGATTTGGTTGGATTTGGTTAAGTCTATGCTGAAGGAAACTCAGTGGTTGTTAAACAATTCGGTGCCGGCACTGGATGAATACATGGAAAATGCATATGTATCATTTGCCTTGGGACCGATTGTGCTTCCAGCTCTCTATTTGGTAGGGCCTAAGCTCTCAGAGGAGGCTGTATGGAGTTTCGAATTCCACCAGTTGTATAAACTTATGAGCACCTGTGGGCGTCTTCTCAATGATATGCAAACCTTTAAGAGGGAATCTGCAGAAGGGAAGCTAAATGCTGTTACATTGGCCATGCTTCATGGCAATGGCAGTGCTACTGAAGAAGAGACCTTCAGTGAGATGAAGAGCATTATAGCCATTAAGAGGAGAGAACTGCAAAGACTAGTTTTGCAGAAGGATAGCTTAGTTCCAAGAGCTTGCAAGGACCTGTTTTGGAACATGTGCAAAATTGTGCACCTATTTTATGCAAAGCACGATGGATTCACTGGGCATGACTTGATGAAAACCGTAAATGGACTAACCGAAGAACCGATCATTCTCAGTGAATTGCAAGTAGAAAGTAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006629 GO:0006720 GO:0006721 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009685 GO:0009686 GO:0009719 GO:0009725 GO:0009739 GO:0009740 GO:0009755 GO:0009899 GO:0009987 GO:0010033 GO:0010333 GO:0010476 GO:0010817 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016829 GO:0016835 GO:0016838 GO:0019752 GO:0023052 GO:0032870 GO:0033993 GO:0042221 GO:0042445 GO:0042446 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0065008 GO:0070887 GO:0071229 GO:0071310 GO:0071370 GO:0071396 GO:0071495 GO:0071704 GO:1901576 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko00904 ko01100 ko01110 map00904 map01100 map01110

Pfam Domains

Protein Families

Terpene_synth Terpene_synth_C

Protein Analysis

795

Amino Acids

89.69

Weight (kDa)

5.36

Isoelectric Point (pI)

48.65

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Terpene_synth	PF01397	224 - 418	8e-43	Terpene synthase, N-terminal domain
Terpene_synth_C	PF03936	496 - 730	2e-70	Terpene synthase family, metal binding domain
Terpene_syn_C_2	PF19086	533 - 727	1.3e-40	Terpene synthase family 2, C-terminal metal binding

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0001708)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G79460
fragaria_vesca	FvH4_3g14116 FvH4_3g14130 FvH4_3g14131 FvH4_3g16270
malus_domestica	MD03G1187200.v1.1 MD10G1215700.v1.1 MD10G1215900.v1.1 MD10G1216200.v1.1
prunus_persica	Prupe.4G128500_v2.0.a1 Prupe.4G128500_v2.0.a1 Prupe.4G128500_v2.0.a1 Prupe.4G128600_v2.0.a1 Prupe.4G128700_v2.0.a1
pyrus_communis	pycom03g13990 pycom10g18220
rosa_chinensis	RchiOBHm_Chr5g0023441 RchiOBHm_Chr5g0023471 RchiOBHm_Chr5g0023641
rosa_laevigata	RLG00000032721
rosa_multiflora	Rmu_co8406553.1_g000001 Rmu_sc0004593.1_g000004 Rmu_sc0005664.1_g000001 Rmu_sc0005664.1_g000005
rosa_roxburghii	Rroxscaffold_1G00055600 Rroxscaffold_1G00055620 Rroxscaffold_1G00055910
rosa_rugosa	Rorug05G0079200 Rorug05G0079300
rosa_samantha	Rh5AG166100 Rh5AG166200 Rh5AG166300 Rh5AG167600 Rh5AG168000 Rh5BG165300 Rh5BG165500 Rh5CG180300 Rh5CG180400 Rh5CG181400 Rh5DG166800 Rh5DG166900 Rh5DG167800
rosa_wichuraiana	Rw0G019600 Rw5G014950 Rw5G014970 Rw5G015110 Rw5G015120 Rw5G015210

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1005
AccB1I	GGYRCC	2 cut(s)	977, 1882
AciI	CCGC	1 cut(s)	1576
AclWI	GGATC	1 cut(s)	1028
AcoI	YGGCCR	1 cut(s)	2109
AcsI	RAATTY	5 cut(s)	232, 436, 520, 1499, 2003
AcuI	CTGAAG	7 cut(s)	429, 1109, 1110, 1665, 1871, 2137, 2160
AcyI	GRCGYC	1 cut(s)	2041
AfaI	GTAC	3 cut(s)	692, 918, 1342
AfiI	CCNNNNNNNGG	6 cut(s)	236, 545, 856, 1402, 2070, 2184
AflIII	ACRYGT	1 cut(s)	2259
AhlI	ACTAGT	2 cut(s)	1654, 2203
AjiI	CACGTC	1 cut(s)	1813
AjnI	CCWGG	1 cut(s)	1783
AjuI	GAANNNNNNNTTGG	4 cut(s)	524, 556, 1690, 1722
AloI	GAACNNNNNNTCC	2 cut(s)	798, 830
Alw21I	GWGCWC	3 cut(s)	1754, 2033, 2277
Alw26I	GTCTC	2 cut(s)	573, 2141
Alw44I	GTGCAC	1 cut(s)	2273
AlwI	GGATC	1 cut(s)	1028
AlwNI	CAGNNNCTG	3 cut(s)	452, 939, 974
Ama87I	CYCGRG	1 cut(s)	1451
AoxI	GGCC	3 cut(s)	1587, 1967, 2109
ApaLI	GTGCAC	1 cut(s)	2273
ApeKI	GCWGC	7 cut(s)	74, 737, 740, 943, 1103, 1538, 1541
ApoI	RAATTY	5 cut(s)	232, 436, 520, 1499, 2003
AseI	ATTAAT	1 cut(s)	1758
Asp700I	GAANNNNTTC	1 cut(s)	1155
AspLEI	GCGC	1 cut(s)	1797
AspS9I	GGNCC	7 cut(s)	220, 308, 708, 1587, 1934, 1967, 2245
AsuC2I	CCSGG	1 cut(s)	238
AsuHPI	GGTGA	3 cut(s)	407, 714, 743
AsuII	TTCGAA	1 cut(s)	2001
AvaI	CYCGRG	1 cut(s)	1451
AvaII	GGWCC	5 cut(s)	220, 308, 708, 1934, 2245
BaeGI	GKGCMC	1 cut(s)	2277
BaeI	ACNNNNGTAYC	2 cut(s)	1439, 1472
BalI	TGGCCA	1 cut(s)	2111
BanI	GGYRCC	2 cut(s)	977, 1882
BarI	GAAGNNNNNNTAC	2 cut(s)	1505, 1537
BbsI	GAAGAC	5 cut(s)	1001, 1062, 1395, 1419, 2036
Bbv12I	GWGCWC	3 cut(s)	1754, 2033, 2277
BbvI	GCAGC	7 cut(s)	86, 724, 727, 930, 1090, 1525, 1553
BccI	CCATC	8 cut(s)	55, 290, 378, 449, 475, 673, 733, 2291
BciT130I	CCWGG	1 cut(s)	1785
BclI	TGATCA	1 cut(s)	1249
BcnI	CCSGG	1 cut(s)	238
BcoDI	GTCTC	2 cut(s)	573, 2141
BcuI	ACTAGT	2 cut(s)	1654, 2203
BfaI	CTAG	6 cut(s)	833, 1160, 1496, 1506, 1655, 2204
BfmI	CTRYAG	4 cut(s)	944, 1029, 1539, 2079
BglII	AGATCT	1 cut(s)	58
BisI	GCNGC	7 cut(s)	75, 738, 741, 944, 1104, 1539, 1542
BlsI	GCNGC	7 cut(s)	76, 739, 742, 945, 1105, 1540, 1543
Bme1390I	CCNGG	2 cut(s)	238, 1785
Bme18I	GGWCC	5 cut(s)	220, 308, 708, 1934, 2245
BmeT110I	CYCGRG	1 cut(s)	1451
BmgBI	CACGTC	1 cut(s)	1813
BmgT120I	GGNCC	7 cut(s)	220, 308, 708, 1587, 1934, 1967, 2245
BmiI	GGNNCC	8 cut(s)	222, 246, 309, 709, 979, 1771, 1884, 1935
BmrFI	CCNGG	2 cut(s)	238, 1785
BmrI	ACTGGG	2 cut(s)	682, 2316
BmsI	GCATC	8 cut(s)	331, 331, 375, 550, 926, 961, 1252, 1561
BmuI	ACTGGG	2 cut(s)	682, 2316
BoxI	GACNNNNGTC	1 cut(s)	414
BpiI	GAAGAC	5 cut(s)	1001, 1062, 1395, 1419, 2036
BplI	GAGNNNNNCTC	2 cut(s)	618, 650
Bpu10I	CCTNAGC	1 cut(s)	1971
Bpu14I	TTCGAA	1 cut(s)	2001
BpuMI	CCSGG	1 cut(s)	238
BsaBI	GATNNNNATC	2 cut(s)	531, 1692
BsaHI	GRCGYC	1 cut(s)	2041
BsaI	GGTCTC	1 cut(s)	2141
BsaJI	CCNNGG	5 cut(s)	89, 1075, 1783, 1784, 1929
Bsc4I	CCNNNNNNNGG	6 cut(s)	236, 545, 856, 1402, 2070, 2184
Bse118I	RCCGGY	1 cut(s)	1885
Bse1I	ACTGG	8 cut(s)	677, 864, 1102, 1181, 1407, 1896, 2011, 2311
Bse3DI	GCAATG	3 cut(s)	222, 522, 2131
Bse8I	GATNNNNATC	2 cut(s)	531, 1692
BseBI	CCWGG	1 cut(s)	1785
BseDI	CCNNGG	5 cut(s)	89, 1075, 1783, 1784, 1929
BseGI	GGATG	6 cut(s)	532, 958, 1141, 1264, 1693, 1900
BseJI	GATNNNNATC	2 cut(s)	531, 1692
BseLI	CCNNNNNNNGG	6 cut(s)	236, 545, 856, 1402, 2070, 2184
BseMI	GCAATG	3 cut(s)	222, 522, 2131
BseMII	CTCAG	7 cut(s)	586, 627, 1235, 1707, 1874, 1992, 2374
BseNI	ACTGG	8 cut(s)	677, 864, 1102, 1181, 1407, 1896, 2011, 2311
BseRI	GAGGAG	2 cut(s)	1997, 2200
BseSI	GKGCMC	1 cut(s)	2277
BseXI	GCAGC	7 cut(s)	86, 724, 727, 930, 1090, 1525, 1553
BseYI	CCCAGC	1 cut(s)	12
BsgI	GTGCAG	1 cut(s)	831
BshFI	GGCC	3 cut(s)	1589, 1969, 2111
BshNI	GGYRCC	2 cut(s)	977, 1882
BsiHKAI	GWGCWC	3 cut(s)	1754, 2033, 2277
BsiHKCI	CYCGRG	1 cut(s)	1451
BsiSI	CCGG	2 cut(s)	237, 1886
BslFI	GGGAC	2 cut(s)	206, 1947
BslI	CCNNNNNNNGG	6 cut(s)	236, 545, 856, 1402, 2070, 2184
BsmAI	GTCTC	2 cut(s)	573, 2141
BsmFI	GGGAC	2 cut(s)	206, 1947
BsmI	GAATGC	2 cut(s)	266, 1778
BsnI	GGCC	3 cut(s)	1589, 1969, 2111
Bso31I	GGTCTC	1 cut(s)	2141
BsoBI	CYCGRG	1 cut(s)	1451
Bsp119I	TTCGAA	1 cut(s)	2001
Bsp1286I	GDGCHC	3 cut(s)	1754, 2033, 2277
Bsp143I	GATC	7 cut(s)	58, 178, 886, 1033, 1249, 1674, 2352
BspACI	CCGC	1 cut(s)	1576
BspANI	GGCC	3 cut(s)	1589, 1969, 2111
BspCNI	CTCAG	7 cut(s)	585, 626, 1234, 1708, 1873, 1991, 2373
BspHI	TCATGA	2 cut(s)	292, 684
BspLI	GGNNCC	8 cut(s)	222, 246, 309, 709, 979, 1771, 1884, 1935
BspMAI	CTGCAG	4 cut(s)	948, 1033, 1543, 2083
BspPI	GGATC	1 cut(s)	1028
BspQI	GCTCTTC	2 cut(s)	1272, 2159
BspT104I	TTCGAA	1 cut(s)	2001
BspT107I	GGYRCC	2 cut(s)	977, 1882
BspTNI	GGTCTC	1 cut(s)	2141
BsrDI	GCAATG	3 cut(s)	222, 522, 2131
BsrFI	RCCGGY	1 cut(s)	1885
BsrI	ACTGG	8 cut(s)	677, 864, 1102, 1181, 1407, 1896, 2011, 2311
BssAI	RCCGGY	1 cut(s)	1885
BssECI	CCNNGG	5 cut(s)	89, 1075, 1783, 1784, 1929
BssMI	GATC	7 cut(s)	58, 178, 886, 1033, 1249, 1674, 2352
BssNI	GRCGYC	1 cut(s)	2041
BssT1I	CCWWGG	3 cut(s)	89, 1075, 1929
Bst2UI	CCWGG	1 cut(s)	1785
Bst4CI	ACNGT	5 cut(s)	85, 1061, 1526, 1612, 2329
Bst6I	CTCTTC	4 cut(s)	1272, 1641, 2139, 2159
BstACI	GRCGYC	1 cut(s)	2041
BstBI	TTCGAA	1 cut(s)	2001
BstC8I	GCNNGC	8 cut(s)	364, 847, 941, 976, 1518, 1576, 1887, 2239
BstENI	CCTNNNNNAGG	2 cut(s)	543, 2068
BstF5I	GGATG	6 cut(s)	532, 958, 1141, 1264, 1693, 1900
BstHHI	GCGC	1 cut(s)	1797
BstKTI	GATC	7 cut(s)	61, 181, 889, 1036, 1252, 1677, 2355
BstMAI	GTCTC	2 cut(s)	573, 2141
BstMBI	GATC	7 cut(s)	58, 178, 886, 1033, 1249, 1674, 2352
BstMWI	GCNNNNNNNGC	3 cut(s)	212, 606, 635
BstNI	CCWGG	1 cut(s)	1785
BstNSI	RCATGY	2 cut(s)	366, 2263
BstPAI	GACNNNNGTC	1 cut(s)	414
BstSCI	CCNGG	2 cut(s)	236, 1783
BstSFI	CTRYAG	4 cut(s)	944, 1029, 1539, 2079
BstSLI	GKGCMC	1 cut(s)	2277
BstV1I	GCAGC	7 cut(s)	86, 724, 727, 930, 1090, 1525, 1553
BstV2I	GAAGAC	5 cut(s)	1001, 1062, 1395, 1419, 2036
BstX2I	RGATCY	2 cut(s)	58, 1033
BstYI	RGATCY	2 cut(s)	58, 1033
BsuRI	GGCC	3 cut(s)	1589, 1969, 2111
BtgZI	GCGATG	1 cut(s)	397
BtrI	CACGTC	1 cut(s)	1813
BtsCI	GGATG	6 cut(s)	532, 958, 1141, 1264, 1693, 1900
BtsI	GCAGTG	4 cut(s)	201, 1413, 1804, 2137
Cac8I	GCNNGC	8 cut(s)	364, 847, 941, 976, 1518, 1576, 1887, 2239
CaiI	CAGNNNCTG	3 cut(s)	452, 939, 974
CciI	TCATGA	2 cut(s)	292, 684
CfoI	GCGC	1 cut(s)	1797
Cfr10I	RCCGGY	1 cut(s)	1885
Cfr13I	GGNCC	7 cut(s)	220, 308, 708, 1587, 1934, 1967, 2245
CseI	GACGC	1 cut(s)	2030
Csp6I	GTAC	3 cut(s)	691, 917, 1341
CviQI	GTAC	3 cut(s)	691, 917, 1341
DpnI	GATC	7 cut(s)	60, 180, 888, 1035, 1251, 1676, 2354
DpnII	GATC	7 cut(s)	58, 178, 886, 1033, 1249, 1674, 2352
DraI	TTTAAA	1 cut(s)	1353
DrdI	GACNNNNNNGTC	1 cut(s)	1005
DseDI	GACNNNNNNGTC	1 cut(s)	1005
EaeI	YGGCCR	1 cut(s)	2109
Eam1104I	CTCTTC	4 cut(s)	1272, 1641, 2139, 2159
EarI	CTCTTC	4 cut(s)	1272, 1641, 2139, 2159
Eco130I	CCWWGG	3 cut(s)	89, 1075, 1929
Eco31I	GGTCTC	1 cut(s)	2141
Eco32I	GATATC	1 cut(s)	1085
Eco47I	GGWCC	5 cut(s)	220, 308, 708, 1934, 2245
Eco57I	CTGAAG	7 cut(s)	429, 1109, 1110, 1665, 1871, 2137, 2160
Eco88I	CYCGRG	1 cut(s)	1451
EcoNI	CCTNNNNNAGG	2 cut(s)	543, 2068
EcoO109I	RGGNCCY	4 cut(s)	308, 708, 1967, 2245
EcoRI	GAATTC	1 cut(s)	2003
EcoRII	CCWGG	1 cut(s)	1783
EcoRV	GATATC	1 cut(s)	1085
EcoT14I	CCWWGG	3 cut(s)	89, 1075, 1929
EcoT22I	ATGCAT	3 cut(s)	368, 1780, 1915
ErhI	CCWWGG	3 cut(s)	89, 1075, 1929
FalI	AAGNNNNNCTT	2 cut(s)	2207, 2239
FaqI	GGGAC	2 cut(s)	206, 1947
FauI	CCCGC	1 cut(s)	1583
FauNDI	CATATG	2 cut(s)	196, 1915
FbaI	TGATCA	1 cut(s)	1249
Fnu4HI	GCNGC	7 cut(s)	75, 738, 741, 944, 1104, 1539, 1542
FokI	GGATG	6 cut(s)	539, 965, 1128, 1271, 1700, 1907
Fsp4HI	GCNGC	7 cut(s)	75, 738, 741, 944, 1104, 1539, 1542
FspBI	CTAG	6 cut(s)	833, 1160, 1496, 1506, 1655, 2204
GlaI	GCGC	1 cut(s)	1796
GluI	GCNGC	7 cut(s)	75, 738, 741, 944, 1104, 1539, 1542
GsaI	CCCAGC	1 cut(s)	16
HaeIII	GGCC	3 cut(s)	1589, 1969, 2111
HapII	CCGG	2 cut(s)	237, 1886
HgaI	GACGC	1 cut(s)	2030
HhaI	GCGC	1 cut(s)	1797
Hin1I	GRCGYC	1 cut(s)	2041
Hin6I	GCGC	1 cut(s)	1795
HinP1I	GCGC	1 cut(s)	1795
HincII	GTYRAC	1 cut(s)	333
HindII	GTYRAC	1 cut(s)	333
HpaI	GTTAAC	1 cut(s)	333
HpaII	CCGG	2 cut(s)	237, 1886
HphI	GGTGA	3 cut(s)	407, 714, 743
Hpy166II	GTNNAC	4 cut(s)	333, 917, 1660, 2275
Hpy8I	GTNNAC	4 cut(s)	333, 917, 1660, 2275
HpyCH4III	ACNGT	5 cut(s)	85, 1061, 1526, 1612, 2329
HpyCH4IV	ACGT	4 cut(s)	356, 1006, 1632, 1812
HpyF10VI	GCNNNNNNNGC	3 cut(s)	212, 606, 635
HpySE526I	ACGT	4 cut(s)	356, 1006, 1632, 1812
Hsp92I	GRCGYC	1 cut(s)	2041
HspAI	GCGC	1 cut(s)	1795
KroI	GCCGGC	1 cut(s)	1885
KroNI	GCCGGC	1 cut(s)	1887
Ksp22I	TGATCA	1 cut(s)	1249
KspAI	GTTAAC	1 cut(s)	333
Kzo9I	GATC	7 cut(s)	58, 178, 886, 1033, 1249, 1674, 2352
LguI	GCTCTTC	2 cut(s)	1272, 2159
LmnI	GCTCC	2 cut(s)	244, 1769
Lsp1109I	GCAGC	7 cut(s)	86, 724, 727, 930, 1090, 1525, 1553
LweI	GCATC	8 cut(s)	331, 331, 375, 550, 926, 961, 1252, 1561
MaeI	CTAG	6 cut(s)	833, 1160, 1496, 1506, 1655, 2204
MaeII	ACGT	4 cut(s)	356, 1006, 1632, 1812
MaeIII	GTNAC	4 cut(s)	663, 1526, 1801, 2101
MalI	GATC	7 cut(s)	60, 180, 888, 1035, 1251, 1676, 2354
MboI	GATC	7 cut(s)	58, 178, 886, 1033, 1249, 1674, 2352
MfeI	CAATTG	2 cut(s)	1430, 1670
MflI	RGATCY	2 cut(s)	58, 1033
MhlI	GDGCHC	3 cut(s)	1754, 2033, 2277
MlsI	TGGCCA	1 cut(s)	2111
MluNI	TGGCCA	1 cut(s)	2111
MlyI	GAGTC	2 cut(s)	440, 993
MmeI	TCCRAC	3 cut(s)	842, 1615, 1809
Mox20I	TGGCCA	1 cut(s)	2111
Mph1103I	ATGCAT	3 cut(s)	368, 1780, 1915
MroNI	GCCGGC	1 cut(s)	1885
MroXI	GAANNNNTTC	1 cut(s)	1155
MscI	TGGCCA	1 cut(s)	2111
MslI	CAYNNNNRTG	4 cut(s)	934, 1017, 1146, 2124
Msp20I	TGGCCA	1 cut(s)	2111
MspI	CCGG	2 cut(s)	237, 1886
MspR9I	CCNGG	2 cut(s)	238, 1785
MunI	CAATTG	2 cut(s)	1430, 1670
Mva1269I	GAATGC	2 cut(s)	266, 1778
MvaI	CCWGG	1 cut(s)	1785
MwoI	GCNNNNNNNGC	3 cut(s)	212, 606, 635
NaeI	GCCGGC	1 cut(s)	1887
NciI	CCSGG	1 cut(s)	238
NdeI	CATATG	2 cut(s)	196, 1915
NdeII	GATC	7 cut(s)	58, 178, 886, 1033, 1249, 1674, 2352
NgoMIV	GCCGGC	1 cut(s)	1885
NlaIV	GGNNCC	8 cut(s)	222, 246, 309, 709, 979, 1771, 1884, 1935
NmuCI	GTSAC	1 cut(s)	663
NsiI	ATGCAT	3 cut(s)	368, 1780, 1915
NspI	RCATGY	2 cut(s)	366, 2263
NspV	TTCGAA	1 cut(s)	2001
PaeI	GCATGC	1 cut(s)	366
PaeR7I	CTCGAG	1 cut(s)	1451
PagI	TCATGA	2 cut(s)	292, 684
PasI	CCCWGGG	1 cut(s)	1784
PciI	ACATGT	1 cut(s)	2259
PciSI	GCTCTTC	2 cut(s)	1272, 2159
PcsI	WCGNNNNNNNCGW	1 cut(s)	1003
PctI	GAATGC	2 cut(s)	266, 1778
PdiI	GCCGGC	1 cut(s)	1887
PdmI	GAANNNNTTC	1 cut(s)	1155
PfeI	GAWTC	8 cut(s)	509, 532, 654, 1151, 1693, 1706, 2075, 2301
PfoI	TCCNGGA	1 cut(s)	236
PkrI	GCNGC	7 cut(s)	76, 739, 742, 945, 1105, 1540, 1543
PleI	GAGTC	2 cut(s)	439, 993
PpsI	GAGTC	2 cut(s)	439, 993
PpuMI	RGGWCCY	3 cut(s)	308, 708, 2245
PscI	ACATGT	1 cut(s)	2259
PshAI	GACNNNNGTC	1 cut(s)	414
PshBI	ATTAAT	1 cut(s)	1758
Psp5II	RGGWCCY	3 cut(s)	308, 708, 2245
Psp6I	CCWGG	1 cut(s)	1783
PspFI	CCCAGC	1 cut(s)	12
PspGI	CCWGG	1 cut(s)	1783
PspN4I	GGNNCC	8 cut(s)	222, 246, 309, 709, 979, 1771, 1884, 1935
PspPI	GGNCC	7 cut(s)	220, 308, 708, 1587, 1934, 1967, 2245
PspPPI	RGGWCCY	3 cut(s)	308, 708, 2245
PspXI	VCTCGAGB	1 cut(s)	1451
PstI	CTGCAG	4 cut(s)	948, 1033, 1543, 2083
PstNI	CAGNNNCTG	3 cut(s)	452, 939, 974
PsuI	RGATCY	2 cut(s)	58, 1033
RsaI	GTAC	3 cut(s)	692, 918, 1342
RsaNI	GTAC	3 cut(s)	691, 917, 1341
RseI	CAYNNNNRTG	4 cut(s)	934, 1017, 1146, 2124
SapI	GCTCTTC	2 cut(s)	1272, 2159
SatI	GCNGC	7 cut(s)	75, 738, 741, 944, 1104, 1539, 1542
Sau3AI	GATC	7 cut(s)	58, 178, 886, 1033, 1249, 1674, 2352
Sau96I	GGNCC	7 cut(s)	220, 308, 708, 1587, 1934, 1967, 2245
SchI	GAGTC	2 cut(s)	440, 993
ScrFI	CCNGG	2 cut(s)	238, 1785
SduI	GDGCHC	3 cut(s)	1754, 2033, 2277
SfaNI	GCATC	8 cut(s)	331, 331, 375, 550, 926, 961, 1252, 1561
SfcI	CTRYAG	4 cut(s)	944, 1029, 1539, 2079
Sfr274I	CTCGAG	1 cut(s)	1451
SfuI	TTCGAA	1 cut(s)	2001
SinI	GGWCC	5 cut(s)	220, 308, 708, 1934, 2245
SlaI	CTCGAG	1 cut(s)	1451
SmiMI	CAYNNNNRTG	4 cut(s)	934, 1017, 1146, 2124
SmlI	CTYRAG	1 cut(s)	1451
SmoI	CTYRAG	1 cut(s)	1451
SpeI	ACTAGT	2 cut(s)	1654, 2203
SphI	GCATGC	1 cut(s)	366
SsiI	CCGC	1 cut(s)	1576
SspI	AATATT	3 cut(s)	391, 492, 1325
SspMI	CTAG	6 cut(s)	833, 1160, 1496, 1506, 1655, 2204
StyD4I	CCNGG	2 cut(s)	236, 1783
StyI	CCWWGG	3 cut(s)	89, 1075, 1929
TaaI	ACNGT	5 cut(s)	85, 1061, 1526, 1612, 2329
TaiI	ACGT	4 cut(s)	359, 1009, 1635, 1815
TaqI	TCGA	7 cut(s)	429, 889, 921, 997, 1452, 1677, 2001
TaqII	GACCGA	1 cut(s)	1951
TatI	WGTACW	2 cut(s)	916, 1340
TfiI	GAWTC	8 cut(s)	509, 532, 654, 1151, 1693, 1706, 2075, 2301
TseFI	GTSAC	1 cut(s)	663
TseI	GCWGC	7 cut(s)	74, 737, 740, 943, 1103, 1538, 1541
Tsp45I	GTSAC	1 cut(s)	663
TspGWI	ACGGA	1 cut(s)	1611
VneI	GTGCAC	1 cut(s)	2273
VpaK11BI	GGWCC	5 cut(s)	220, 308, 708, 1934, 2245
VspI	ATTAAT	1 cut(s)	1758
XagI	CCTNNNNNAGG	2 cut(s)	543, 2068
XapI	RAATTY	5 cut(s)	232, 436, 520, 1499, 2003
XbaI	TCTAGA	2 cut(s)	832, 1159
XceI	RCATGY	2 cut(s)	366, 2263
XcmI	CCANNNNNNNNNTGG	1 cut(s)	701
XhoI	CTCGAG	1 cut(s)	1451
XmnI	GAANNNNTTC	1 cut(s)	1155
XspI	CTAG	6 cut(s)	833, 1160, 1496, 1506, 1655, 2204
Zsp2I	ATGCAT	3 cut(s)	368, 1780, 1915

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.