Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0001708

Physical Location & Seq

Genomic Coordinates Forward (+)

17461756 .. 17467173

Gene Structure 5418 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ30336

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2400 bp

ATGTCCTTCTCCCAGCTCAACACTTTCAGGTGTTGCACCTTTTCCCCATCAGAAAAAAAATTAGGAAAAGTTCTCTTGCCACAAGCAGCTTCAATAGTTCCAAGGACAAATGGCACAGCAGAAGTAAATGCTGCTGTTTTGAGTTTGGATGGAACTAAAAAAAGAATAAAGGAGATGTTCAACAAGGTTGATCTCTCTGTTTCATCATATGACACTGCTTGGGTGGCAATGGTCCCTTCTCCAAATTCCGGGAAGGAGCCTTTCTTCCCTGAATGCGTAAATTGGTTGTTAGATAATCAACTTCATGATGGCTCATGGGGTCCTCCTAATCTGCATCAGTTGTTAACAAAAGATGCCCTCTTATCCACGTTAGCATGCATCCTTGCACTAAAGCGATGGAATATTGGTGAAGAACTAATTGACAAGGGTCTGCATTTTATCGAGTCAAATTTAGCTTCAGCTACTGATCGAGAGCAACCATCTCCTGTTGGGTTTGGTATAATATTTCCTTCCATGATTGAATCTGCAATGAATTTGGATGTGAATCTACCTTTGGGAGGATTAACCTTAGATGCTTTACTTCTCAGGAGAGACTTTGAGCTTCAAAGAGGCAACAGAAGCAACTCAGAGGGGTGGAGAGCCTATCTCGCATATATTTCAGAAGGAATCGGAAAGTCACAGGACTGGGAGATGGTCATGAAGTACCAGAAGAAGAATGGGTCCTTGTTTAACTCACCATCAACTACTGCTGCTGCTTTTACTCACCTCAACAATGCTGGTTGTCTTAGTTATCTACGCTCACTCATAGAAAAGTTCGGAAGTGCAGTTCCAACGATTTATCCTCTAGATAACTATGCTCGCCTTTCTATGGTTTCCAGTCTTGAAAGTATGGGCGTTGATCGACATTTCAGGGAGGAAATAAGGAGTGTACTCGATGAAACATACAGATGCTGGCTGCAGGGGGATGAAGATATATTTTCAGGTGCTGGCACCTGTGCTATGGCATTTCGACTCTTACGTCTTCATGGATATGATGTTTCTGCAGATCCATTAAGTCAATTTTCAGAAGACAGTTTCTTCAATTCCCTTGGAGGATATCTGAAGGACACTGGTGCTGCCTTAGAACTTTTTAGGGCTTCAGAAGTCATCATTCATCCAGATGAATCAGTTCTAGAGAAACAACATTACTGGACAAGTCATTTCTTGAAACAGGAATTATCAAATAACTTAACTCAGGCTCATAAACTCAATAACCATATTGATCAGGTGGATGATGCTCTTCGGTTTCCTTCCTATGCAACTCTGGGTCGGTTATCAAGCAGGAAATCAATAAAATATTACAACACTGATAGTACAAAGATTTTAAAATCTTCTTATCGTTGTTCAAATGTTGGGAATGAAGACTTCCTAAAACTGGCAGTGGAAGACTTCAATATGTGTCAATCGATTCATCGGGAAGAACTCAAGTATCTTTCAAGGTGGGTTGTCGAGAGCAGATTAGACAAGCTAGAATTTGCTAGGCAGAAGCAGGCATACTGTTACTTCTCTGCTGCTGCAACCCTTTTCCCTCCTGAACTATCAGATGCCCGCATATCATGGGCCAAAAACGGAGTGCTTACAACTGTGGTTGATGATTTCTTTGACGTTGGAGGTTCTGAAGAGGAACTAGTAAACCTTATACAATTGATCGAGAAGTGGGATGTGAACGCAAAAACTGATTCTTGTTCTGAGCAAGTTGAAATCATATTTTCAGCAATTAAGAGCACAATTAATGAGATTGGAGCCAATGCATTCCCGCGGCAACAGCGCAGTGTAACAAATCACGTCATAGAGATTTGGTTGGATTTGGTTAAGTCTATGCTGAAGGAAACTCAGTGGTTGTTAAACAATTCGGTGCCGGCACTGGATGAATACATGGAAAATGCATATGTATCATTTGCCTTGGGACCGATTGTGCTTCCAGCTCTCTATTTGGTAGGGCCTAAGCTCTCAGAGGAGGCTGTATGGAGTTTCGAATTCCACCAGTTGTATAAACTTATGAGCACCTGTGGGCGTCTTCTCAATGATATGCAAACCTTTAAGAGGGAATCTGCAGAAGGGAAGCTAAATGCTGTTACATTGGCCATGCTTCATGGCAATGGCAGTGCTACTGAAGAAGAGACCTTCAGTGAGATGAAGAGCATTATAACCATTAAGAGGAGAGAACTGCAAAGACTAGTTTTGCAGAAGGATAGCTTAGTTCCAAGAGCTTGCAAGGACCTGTTTTGGAACATGTGCAAAATTGTGCACCTATTTTATGCAAAGCACGATGGATTCACTGGGCATGACTTGATGAAAACCGTAAATGGACTAACCGAAGAACCGATCATTCTCAGTGAATTGCAAGTAGAAAGTAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006629 GO:0006720 GO:0006721 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009685 GO:0009686 GO:0009719 GO:0009725 GO:0009739 GO:0009740 GO:0009755 GO:0009899 GO:0009987 GO:0010033 GO:0010333 GO:0010476 GO:0010817 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016829 GO:0016835 GO:0016838 GO:0019752 GO:0023052 GO:0032870 GO:0033993 GO:0042221 GO:0042445 GO:0042446 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0065008 GO:0070887 GO:0071229 GO:0071310 GO:0071370 GO:0071396 GO:0071495 GO:0071704 GO:1901576 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko00904 ko01100 ko01110 map00904 map01100 map01110

Pfam Domains

Protein Families

Terpene_synth Terpene_synth_C

Protein Analysis

799

Amino Acids

90.14

Weight (kDa)

5.58

Isoelectric Point (pI)

48.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Terpene_synth	PF01397	228 - 421	2.1e-41	Terpene synthase, N-terminal domain
Terpene_synth_C	PF03936	499 - 734	1e-70	Terpene synthase family, metal binding domain
Terpene_syn_C_2	PF19086	537 - 732	3e-41	Terpene synthase family 2, C-terminal metal binding

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0001708)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G79460
fragaria_vesca	FvH4_3g14116 FvH4_3g14130 FvH4_3g14131 FvH4_3g16270
malus_domestica	MD03G1187200.v1.1 MD10G1215700.v1.1 MD10G1215900.v1.1 MD10G1216200.v1.1
prunus_persica	Prupe.4G128500_v2.0.a1 Prupe.4G128500_v2.0.a1 Prupe.4G128500_v2.0.a1 Prupe.4G128600_v2.0.a1 Prupe.4G128700_v2.0.a1
pyrus_communis	pycom03g13990 pycom10g18220
rosa_chinensis	RchiOBHm_Chr5g0023441 RchiOBHm_Chr5g0023471 RchiOBHm_Chr5g0023641
rosa_laevigata	RLG00000032721
rosa_multiflora	Rmu_co8406553.1_g000001 Rmu_sc0004593.1_g000004 Rmu_sc0005664.1_g000001 Rmu_sc0005664.1_g000005
rosa_roxburghii	Rroxscaffold_1G00055600 Rroxscaffold_1G00055620 Rroxscaffold_1G00055910
rosa_rugosa	Rorug05G0079200 Rorug05G0079300
rosa_samantha	Rh5AG166100 Rh5AG166200 Rh5AG166300 Rh5AG167600 Rh5AG168000 Rh5BG165300 Rh5BG165500 Rh5CG180300 Rh5CG180400 Rh5CG181400 Rh5DG166800 Rh5DG166900 Rh5DG167800
rosa_wichuraiana	Rw0G019600 Rw5G014950 Rw5G014970 Rw5G015110 Rw5G015120 Rw5G015210

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2186
AasI	GACNNNNNNGTC	1 cut(s)	1017
AccB1I	GGYRCC	2 cut(s)	989, 1894
AccII	CGCG	1 cut(s)	1798
AciI	CCGC	3 cut(s)	1588, 1796, 1798
AclWI	GGATC	1 cut(s)	1040
AcoI	YGGCCR	1 cut(s)	2121
AcsI	RAATTY	5 cut(s)	244, 448, 532, 1511, 2015
AcuI	CTGAAG	7 cut(s)	441, 1121, 1122, 1677, 1883, 2149, 2172
AcyI	GRCGYC	1 cut(s)	2053
AfaI	GTAC	3 cut(s)	704, 930, 1354
AfiI	CCNNNNNNNGG	6 cut(s)	248, 557, 868, 1414, 2082, 2196
AflIII	ACRYGT	1 cut(s)	2271
AhlI	ACTAGT	2 cut(s)	1666, 2215
AjiI	CACGTC	1 cut(s)	1825
AjuI	GAANNNNNNNTTGG	2 cut(s)	536, 568
AloI	GAACNNNNNNTCC	2 cut(s)	810, 842
Alw21I	GWGCWC	3 cut(s)	1766, 2045, 2289
Alw26I	GTCTC	2 cut(s)	585, 2153
Alw44I	GTGCAC	1 cut(s)	2285
AlwI	GGATC	1 cut(s)	1040
AlwNI	CAGNNNCTG	3 cut(s)	464, 951, 986
AoxI	GGCC	3 cut(s)	1599, 1979, 2121
ApaLI	GTGCAC	1 cut(s)	2285
ApeKI	GCWGC	8 cut(s)	86, 131, 749, 752, 955, 1115, 1550, 1553
ApoI	RAATTY	5 cut(s)	244, 448, 532, 1511, 2015
AseI	ATTAAT	1 cut(s)	1770
Asp700I	GAANNNNTTC	1 cut(s)	1167
AspLEI	GCGC	1 cut(s)	1809
AspS9I	GGNCC	7 cut(s)	232, 320, 720, 1599, 1946, 1979, 2257
AsuC2I	CCSGG	1 cut(s)	250
AsuHPI	GGTGA	3 cut(s)	419, 726, 755
AsuII	TTCGAA	1 cut(s)	2013
AvaII	GGWCC	5 cut(s)	232, 320, 720, 1946, 2257
BaeGI	GKGCMC	1 cut(s)	2289
BaeI	ACNNNNGTAYC	2 cut(s)	1451, 1484
BalI	TGGCCA	1 cut(s)	2123
BanI	GGYRCC	2 cut(s)	989, 1894
BarI	GAAGNNNNNNTAC	2 cut(s)	1517, 1549
BbsI	GAAGAC	5 cut(s)	1013, 1074, 1407, 1431, 2048
Bbv12I	GWGCWC	3 cut(s)	1766, 2045, 2289
BbvI	GCAGC	8 cut(s)	98, 118, 736, 739, 942, 1102, 1537, 1540
BccI	CCATC	8 cut(s)	55, 143, 302, 390, 487, 685, 745, 2303
BclI	TGATCA	1 cut(s)	1261
BcnI	CCSGG	1 cut(s)	250
BcoDI	GTCTC	2 cut(s)	585, 2153
BcuI	ACTAGT	2 cut(s)	1666, 2215
BfaI	CTAG	6 cut(s)	845, 1172, 1508, 1518, 1667, 2216
BfmI	CTRYAG	3 cut(s)	956, 1041, 2091
BisI	GCNGC	9 cut(s)	87, 132, 750, 753, 956, 1116, 1551, 1554, 1799
BlsI	GCNGC	9 cut(s)	88, 133, 751, 754, 957, 1117, 1552, 1555, 1800
Bme1390I	CCNGG	1 cut(s)	250
Bme18I	GGWCC	5 cut(s)	232, 320, 720, 1946, 2257
BmgBI	CACGTC	1 cut(s)	1825
BmgT120I	GGNCC	7 cut(s)	232, 320, 720, 1599, 1946, 1979, 2257
BmiI	GGNNCC	8 cut(s)	234, 258, 321, 721, 991, 1783, 1896, 1947
BmrFI	CCNGG	1 cut(s)	250
BmrI	ACTGGG	2 cut(s)	694, 2328
BmsI	GCATC	7 cut(s)	343, 343, 387, 562, 938, 1264, 1573
BmuI	ACTGGG	2 cut(s)	694, 2328
BoxI	GACNNNNGTC	1 cut(s)	426
BpiI	GAAGAC	5 cut(s)	1013, 1074, 1407, 1431, 2048
BplI	GAGNNNNNCTC	2 cut(s)	630, 662
Bpu10I	CCTNAGC	1 cut(s)	1983
Bpu14I	TTCGAA	1 cut(s)	2013
BpuEI	CTTGAG	1 cut(s)	1448
BpuMI	CCSGG	1 cut(s)	250
Bsa29I	ATCGAT	1 cut(s)	1445
BsaBI	GATNNNNATC	1 cut(s)	543
BsaHI	GRCGYC	1 cut(s)	2053
BsaI	GGTCTC	1 cut(s)	2153
BsaJI	CCNNGG	4 cut(s)	101, 1087, 1796, 1941
Bsc4I	CCNNNNNNNGG	6 cut(s)	248, 557, 868, 1414, 2082, 2196
Bse118I	RCCGGY	1 cut(s)	1897
Bse1I	ACTGG	8 cut(s)	689, 876, 1114, 1193, 1419, 1908, 2023, 2323
Bse3DI	GCAATG	3 cut(s)	234, 534, 2143
Bse8I	GATNNNNATC	1 cut(s)	543
BseCI	ATCGAT	1 cut(s)	1445
BseDI	CCNNGG	4 cut(s)	101, 1087, 1796, 1941
BseGI	GGATG	8 cut(s)	154, 378, 544, 970, 1153, 1276, 1705, 1912
BseJI	GATNNNNATC	1 cut(s)	543
BseLI	CCNNNNNNNGG	6 cut(s)	248, 557, 868, 1414, 2082, 2196
BseMI	GCAATG	3 cut(s)	234, 534, 2143
BseMII	CTCAG	7 cut(s)	598, 639, 1247, 1719, 1886, 2004, 2386
BseNI	ACTGG	8 cut(s)	689, 876, 1114, 1193, 1419, 1908, 2023, 2323
BseRI	GAGGAG	2 cut(s)	2009, 2212
BseSI	GKGCMC	1 cut(s)	2289
BseXI	GCAGC	8 cut(s)	98, 118, 736, 739, 942, 1102, 1537, 1540
BseYI	CCCAGC	1 cut(s)	12
BsgI	GTGCAG	1 cut(s)	843
Bsh1236I	CGCG	1 cut(s)	1798
BshFI	GGCC	3 cut(s)	1601, 1981, 2123
BshNI	GGYRCC	2 cut(s)	989, 1894
BshVI	ATCGAT	1 cut(s)	1445
BsiHKAI	GWGCWC	3 cut(s)	1766, 2045, 2289
BsiSI	CCGG	2 cut(s)	249, 1898
BslFI	GGGAC	2 cut(s)	218, 1959
BslI	CCNNNNNNNGG	6 cut(s)	248, 557, 868, 1414, 2082, 2196
BsmAI	GTCTC	2 cut(s)	585, 2153
BsmFI	GGGAC	2 cut(s)	218, 1959
BsmI	GAATGC	2 cut(s)	278, 1790
BsnI	GGCC	3 cut(s)	1601, 1981, 2123
Bso31I	GGTCTC	1 cut(s)	2153
Bsp119I	TTCGAA	1 cut(s)	2013
Bsp1286I	GDGCHC	3 cut(s)	1766, 2045, 2289
Bsp143I	GATC	7 cut(s)	190, 466, 898, 1045, 1261, 1686, 2364
BspACI	CCGC	3 cut(s)	1588, 1796, 1798
BspANI	GGCC	3 cut(s)	1601, 1981, 2123
BspCNI	CTCAG	7 cut(s)	597, 638, 1246, 1720, 1885, 2003, 2385
BspDI	ATCGAT	1 cut(s)	1445
BspFNI	CGCG	1 cut(s)	1798
BspHI	TCATGA	2 cut(s)	304, 696
BspLI	GGNNCC	8 cut(s)	234, 258, 321, 721, 991, 1783, 1896, 1947
BspMAI	CTGCAG	3 cut(s)	960, 1045, 2095
BspPI	GGATC	1 cut(s)	1040
BspQI	GCTCTTC	2 cut(s)	1284, 2171
BspT104I	TTCGAA	1 cut(s)	2013
BspT107I	GGYRCC	2 cut(s)	989, 1894
BspTNI	GGTCTC	1 cut(s)	2153
BsrDI	GCAATG	3 cut(s)	234, 534, 2143
BsrFI	RCCGGY	1 cut(s)	1897
BsrI	ACTGG	8 cut(s)	689, 876, 1114, 1193, 1419, 1908, 2023, 2323
BssAI	RCCGGY	1 cut(s)	1897
BssECI	CCNNGG	4 cut(s)	101, 1087, 1796, 1941
BssMI	GATC	7 cut(s)	190, 466, 898, 1045, 1261, 1686, 2364
BssNI	GRCGYC	1 cut(s)	2053
BssT1I	CCWWGG	3 cut(s)	101, 1087, 1941
Bst4CI	ACNGT	4 cut(s)	1073, 1538, 1624, 2341
Bst6I	CTCTTC	4 cut(s)	1284, 1653, 2151, 2171
BstACI	GRCGYC	1 cut(s)	2053
BstBI	TTCGAA	1 cut(s)	2013
BstC8I	GCNNGC	8 cut(s)	376, 859, 953, 988, 1530, 1588, 1899, 2251
BstDSI	CCRYGG	1 cut(s)	1796
BstENI	CCTNNNNNAGG	2 cut(s)	555, 2080
BstF5I	GGATG	8 cut(s)	154, 378, 544, 970, 1153, 1276, 1705, 1912
BstFNI	CGCG	1 cut(s)	1798
BstHHI	GCGC	1 cut(s)	1809
BstKTI	GATC	7 cut(s)	193, 469, 901, 1048, 1264, 1689, 2367
BstMAI	GTCTC	2 cut(s)	585, 2153
BstMBI	GATC	7 cut(s)	190, 466, 898, 1045, 1261, 1686, 2364
BstMWI	GCNNNNNNNGC	4 cut(s)	224, 618, 647, 1804
BstNSI	RCATGY	2 cut(s)	378, 2275
BstPAI	GACNNNNGTC	1 cut(s)	426
BstSCI	CCNGG	1 cut(s)	248
BstSFI	CTRYAG	3 cut(s)	956, 1041, 2091
BstSLI	GKGCMC	1 cut(s)	2289
BstUI	CGCG	1 cut(s)	1798
BstV1I	GCAGC	8 cut(s)	98, 118, 736, 739, 942, 1102, 1537, 1540
BstV2I	GAAGAC	5 cut(s)	1013, 1074, 1407, 1431, 2048
BstX2I	RGATCY	1 cut(s)	1045
BstYI	RGATCY	1 cut(s)	1045
Bsu15I	ATCGAT	1 cut(s)	1445
BsuRI	GGCC	3 cut(s)	1601, 1981, 2123
BsuTUI	ATCGAT	1 cut(s)	1445
BtgI	CCRYGG	1 cut(s)	1796
BtgZI	GCGATG	1 cut(s)	409
BtrI	CACGTC	1 cut(s)	1825
BtsCI	GGATG	8 cut(s)	154, 378, 544, 970, 1153, 1276, 1705, 1912
BtsI	GCAGTG	4 cut(s)	213, 1425, 1816, 2149
Cac8I	GCNNGC	8 cut(s)	376, 859, 953, 988, 1530, 1588, 1899, 2251
CaiI	CAGNNNCTG	3 cut(s)	464, 951, 986
CciI	TCATGA	2 cut(s)	304, 696
CfoI	GCGC	1 cut(s)	1809
Cfr10I	RCCGGY	1 cut(s)	1897
Cfr13I	GGNCC	7 cut(s)	232, 320, 720, 1599, 1946, 1979, 2257
Cfr42I	CCGCGG	1 cut(s)	1799
ClaI	ATCGAT	1 cut(s)	1445
CseI	GACGC	1 cut(s)	2042
Csp6I	GTAC	3 cut(s)	703, 929, 1353
CviQI	GTAC	3 cut(s)	703, 929, 1353
DpnI	GATC	7 cut(s)	192, 468, 900, 1047, 1263, 1688, 2366
DpnII	GATC	7 cut(s)	190, 466, 898, 1045, 1261, 1686, 2364
DraI	TTTAAA	1 cut(s)	1365
DrdI	GACNNNNNNGTC	1 cut(s)	1017
DseDI	GACNNNNNNGTC	1 cut(s)	1017
EaeI	YGGCCR	1 cut(s)	2121
Eam1104I	CTCTTC	4 cut(s)	1284, 1653, 2151, 2171
EarI	CTCTTC	4 cut(s)	1284, 1653, 2151, 2171
Eco130I	CCWWGG	3 cut(s)	101, 1087, 1941
Eco31I	GGTCTC	1 cut(s)	2153
Eco32I	GATATC	1 cut(s)	1097
Eco47I	GGWCC	5 cut(s)	232, 320, 720, 1946, 2257
Eco57I	CTGAAG	7 cut(s)	441, 1121, 1122, 1677, 1883, 2149, 2172
EcoNI	CCTNNNNNAGG	2 cut(s)	555, 2080
EcoO109I	RGGNCCY	4 cut(s)	320, 720, 1979, 2257
EcoRI	GAATTC	1 cut(s)	2015
EcoRV	GATATC	1 cut(s)	1097
EcoT14I	CCWWGG	3 cut(s)	101, 1087, 1941
EcoT22I	ATGCAT	3 cut(s)	380, 1792, 1927
ErhI	CCWWGG	3 cut(s)	101, 1087, 1941
FalI	AAGNNNNNCTT	2 cut(s)	2219, 2251
FaqI	GGGAC	2 cut(s)	218, 1959
FauI	CCCGC	2 cut(s)	1595, 1803
FauNDI	CATATG	2 cut(s)	208, 1927
FbaI	TGATCA	1 cut(s)	1261
Fnu4HI	GCNGC	9 cut(s)	87, 132, 750, 753, 956, 1116, 1551, 1554, 1799
FokI	GGATG	8 cut(s)	161, 365, 551, 977, 1140, 1283, 1712, 1919
Fsp4HI	GCNGC	9 cut(s)	87, 132, 750, 753, 956, 1116, 1551, 1554, 1799
FspBI	CTAG	6 cut(s)	845, 1172, 1508, 1518, 1667, 2216
GlaI	GCGC	1 cut(s)	1808
GluI	GCNGC	9 cut(s)	87, 132, 750, 753, 956, 1116, 1551, 1554, 1799
GsaI	CCCAGC	1 cut(s)	16
HaeIII	GGCC	3 cut(s)	1601, 1981, 2123
HapII	CCGG	2 cut(s)	249, 1898
HgaI	GACGC	1 cut(s)	2042
HhaI	GCGC	1 cut(s)	1809
Hin1I	GRCGYC	1 cut(s)	2053
Hin6I	GCGC	1 cut(s)	1807
HinP1I	GCGC	1 cut(s)	1807
HincII	GTYRAC	1 cut(s)	345
HindII	GTYRAC	1 cut(s)	345
HpaI	GTTAAC	1 cut(s)	345
HpaII	CCGG	2 cut(s)	249, 1898
HphI	GGTGA	3 cut(s)	419, 726, 755
Hpy166II	GTNNAC	5 cut(s)	345, 929, 1672, 1705, 2287
Hpy8I	GTNNAC	5 cut(s)	345, 929, 1672, 1705, 2287
HpyCH4III	ACNGT	4 cut(s)	1073, 1538, 1624, 2341
HpyCH4IV	ACGT	4 cut(s)	368, 1018, 1644, 1824
HpyF10VI	GCNNNNNNNGC	4 cut(s)	224, 618, 647, 1804
HpySE526I	ACGT	4 cut(s)	368, 1018, 1644, 1824
Hsp92I	GRCGYC	1 cut(s)	2053
HspAI	GCGC	1 cut(s)	1807
KroI	GCCGGC	1 cut(s)	1897
KroNI	GCCGGC	1 cut(s)	1899
Ksp22I	TGATCA	1 cut(s)	1261
KspAI	GTTAAC	1 cut(s)	345
KspI	CCGCGG	1 cut(s)	1799
Kzo9I	GATC	7 cut(s)	190, 466, 898, 1045, 1261, 1686, 2364
LguI	GCTCTTC	2 cut(s)	1284, 2171
LmnI	GCTCC	2 cut(s)	256, 1781
Lsp1109I	GCAGC	8 cut(s)	98, 118, 736, 739, 942, 1102, 1537, 1540
LweI	GCATC	7 cut(s)	343, 343, 387, 562, 938, 1264, 1573
MaeI	CTAG	6 cut(s)	845, 1172, 1508, 1518, 1667, 2216
MaeII	ACGT	4 cut(s)	368, 1018, 1644, 1824
MaeIII	GTNAC	4 cut(s)	675, 1538, 1813, 2113
MalI	GATC	7 cut(s)	192, 468, 900, 1047, 1263, 1688, 2366
MboI	GATC	7 cut(s)	190, 466, 898, 1045, 1261, 1686, 2364
MfeI	CAATTG	1 cut(s)	1682
MflI	RGATCY	1 cut(s)	1045
MhlI	GDGCHC	3 cut(s)	1766, 2045, 2289
MlsI	TGGCCA	1 cut(s)	2123
MluNI	TGGCCA	1 cut(s)	2123
MlyI	GAGTC	2 cut(s)	452, 1005
MmeI	TCCRAC	3 cut(s)	854, 1627, 1821
Mox20I	TGGCCA	1 cut(s)	2123
Mph1103I	ATGCAT	3 cut(s)	380, 1792, 1927
MroNI	GCCGGC	1 cut(s)	1897
MroXI	GAANNNNTTC	1 cut(s)	1167
MscI	TGGCCA	1 cut(s)	2123
MslI	CAYNNNNRTG	4 cut(s)	946, 1029, 1158, 2136
Msp20I	TGGCCA	1 cut(s)	2123
MspA1I	CMGCKG	1 cut(s)	1798
MspI	CCGG	2 cut(s)	249, 1898
MspR9I	CCNGG	1 cut(s)	250
MunI	CAATTG	1 cut(s)	1682
Mva1269I	GAATGC	2 cut(s)	278, 1790
MvnI	CGCG	1 cut(s)	1798
MwoI	GCNNNNNNNGC	4 cut(s)	224, 618, 647, 1804
NaeI	GCCGGC	1 cut(s)	1899
NciI	CCSGG	1 cut(s)	250
NdeI	CATATG	2 cut(s)	208, 1927
NdeII	GATC	7 cut(s)	190, 466, 898, 1045, 1261, 1686, 2364
NgoMIV	GCCGGC	1 cut(s)	1897
NlaIV	GGNNCC	8 cut(s)	234, 258, 321, 721, 991, 1783, 1896, 1947
NmuCI	GTSAC	1 cut(s)	675
NsiI	ATGCAT	3 cut(s)	380, 1792, 1927
NspI	RCATGY	2 cut(s)	378, 2275
NspV	TTCGAA	1 cut(s)	2013
PaeI	GCATGC	1 cut(s)	378
PagI	TCATGA	2 cut(s)	304, 696
PciI	ACATGT	1 cut(s)	2271
PciSI	GCTCTTC	2 cut(s)	1284, 2171
PcsI	WCGNNNNNNNCGW	1 cut(s)	1015
PctI	GAATGC	2 cut(s)	278, 1790
PdiI	GCCGGC	1 cut(s)	1899
PdmI	GAANNNNTTC	1 cut(s)	1167
PfeI	GAWTC	8 cut(s)	521, 544, 666, 1163, 1447, 1718, 2087, 2313
PfoI	TCCNGGA	1 cut(s)	248
PkrI	GCNGC	9 cut(s)	88, 133, 751, 754, 957, 1117, 1552, 1555, 1800
PleI	GAGTC	2 cut(s)	451, 1005
PpsI	GAGTC	2 cut(s)	451, 1005
PpuMI	RGGWCCY	3 cut(s)	320, 720, 2257
PscI	ACATGT	1 cut(s)	2271
PshAI	GACNNNNGTC	1 cut(s)	426
PshBI	ATTAAT	1 cut(s)	1770
PsiI	TTATAA	1 cut(s)	2186
Psp5II	RGGWCCY	3 cut(s)	320, 720, 2257
PspFI	CCCAGC	1 cut(s)	12
PspN4I	GGNNCC	8 cut(s)	234, 258, 321, 721, 991, 1783, 1896, 1947
PspPI	GGNCC	7 cut(s)	232, 320, 720, 1599, 1946, 1979, 2257
PspPPI	RGGWCCY	3 cut(s)	320, 720, 2257
PstI	CTGCAG	3 cut(s)	960, 1045, 2095
PstNI	CAGNNNCTG	3 cut(s)	464, 951, 986
PsuI	RGATCY	1 cut(s)	1045
RsaI	GTAC	3 cut(s)	704, 930, 1354
RsaNI	GTAC	3 cut(s)	703, 929, 1353
RseI	CAYNNNNRTG	4 cut(s)	946, 1029, 1158, 2136
SacII	CCGCGG	1 cut(s)	1799
SapI	GCTCTTC	2 cut(s)	1284, 2171
SatI	GCNGC	9 cut(s)	87, 132, 750, 753, 956, 1116, 1551, 1554, 1799
Sau3AI	GATC	7 cut(s)	190, 466, 898, 1045, 1261, 1686, 2364
Sau96I	GGNCC	7 cut(s)	232, 320, 720, 1599, 1946, 1979, 2257
SchI	GAGTC	2 cut(s)	452, 1005
ScrFI	CCNGG	1 cut(s)	250
SduI	GDGCHC	3 cut(s)	1766, 2045, 2289
SfaNI	GCATC	7 cut(s)	343, 343, 387, 562, 938, 1264, 1573
SfcI	CTRYAG	3 cut(s)	956, 1041, 2091
Sfr303I	CCGCGG	1 cut(s)	1799
SfuI	TTCGAA	1 cut(s)	2013
SgrBI	CCGCGG	1 cut(s)	1799
SinI	GGWCC	5 cut(s)	232, 320, 720, 1946, 2257
SmiMI	CAYNNNNRTG	4 cut(s)	946, 1029, 1158, 2136
SmlI	CTYRAG	1 cut(s)	1463
SmoI	CTYRAG	1 cut(s)	1463
SpeI	ACTAGT	2 cut(s)	1666, 2215
SphI	GCATGC	1 cut(s)	378
SsiI	CCGC	3 cut(s)	1588, 1796, 1798
SspI	AATATT	3 cut(s)	403, 504, 1337
SspMI	CTAG	6 cut(s)	845, 1172, 1508, 1518, 1667, 2216
StyD4I	CCNGG	1 cut(s)	248
StyI	CCWWGG	3 cut(s)	101, 1087, 1941
TaaI	ACNGT	4 cut(s)	1073, 1538, 1624, 2341
TaiI	ACGT	4 cut(s)	371, 1021, 1647, 1827
TaqI	TCGA	9 cut(s)	441, 469, 901, 933, 1009, 1445, 1488, 1689, 2013
TaqII	GACCGA	1 cut(s)	1963
TatI	WGTACW	2 cut(s)	928, 1352
TauI	GCSGC	1 cut(s)	1801
TfiI	GAWTC	8 cut(s)	521, 544, 666, 1163, 1447, 1718, 2087, 2313
TseFI	GTSAC	1 cut(s)	675
TseI	GCWGC	8 cut(s)	86, 131, 749, 752, 955, 1115, 1550, 1553
Tsp45I	GTSAC	1 cut(s)	675
TspGWI	ACGGA	1 cut(s)	1623
VneI	GTGCAC	1 cut(s)	2285
VpaK11BI	GGWCC	5 cut(s)	232, 320, 720, 1946, 2257
VspI	ATTAAT	1 cut(s)	1770
XagI	CCTNNNNNAGG	2 cut(s)	555, 2080
XapI	RAATTY	5 cut(s)	244, 448, 532, 1511, 2015
XbaI	TCTAGA	2 cut(s)	844, 1171
XceI	RCATGY	2 cut(s)	378, 2275
XcmI	CCANNNNNNNNNTGG	1 cut(s)	713
XmnI	GAANNNNTTC	1 cut(s)	1167
XspI	CTAG	6 cut(s)	845, 1172, 1508, 1518, 1667, 2216
Zsp2I	ATGCAT	3 cut(s)	380, 1792, 1927

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.