Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5B

Orthogroup ID

OG0001708

Physical Location & Seq

Genomic Coordinates Forward (+)

17266675 .. 17271604

Gene Structure 4930 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5BG165300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2379 bp

ATGTCCTTCTCCAACCTCAACATTTTGAGGTGTTGCACACCTTCCTCATCAGGAAAAGATCTCTTGCCACGAGTAGAAGTAAATACTGCTGTTTTGAATTTTGAGGCAACTAAACAAAGAATAAAGAAGATGTTCAACAAGGTTGATCTCTCTGTTTCCTCATATGACACTGCTTGGGTGGCAATGGTCCCTTCTCCAAATTCTGAAAAGGAGCCTTTTTTCCCTGAATGCGTAAATTGGTTATTGGATAATCAACTCCATGATGGCTCATGGGGTCCTCCTAATTTGCATCCATTGTTAACAAAAGATGCTCTTTCATCCACCTTGGCATGTATCCTTGCACTAAAGCAATGGAGTGTCGGTGAAAAACAAATTAACAAGGGGATGCATTTTATTGAGTCAAACTTAGCTTCAGCTACTGATGAAGAGCAGCCATCGCCTGTTGGGTTTGATATAATATTTCCTGCTATGATTGAATCTGCAATGAGTTTGAATATGAATCTTCCCCAGGGTGGATCAACCTTAAAGGCTTTATTTCACAGGAGAGACTTTGAGCTTAAAAGAGGCTATGGAAGCAACTCAGAGGGGTGGAAAGCCTATATAGCATATATTTCAGAAGGAATTGGAAAGTCACAGGACTGGAAGATGGTCATGAAATACCAAAGGAAGAATGGGTCTTTGTTTAATTCACCATCAACTACTGCTTCTGCTTATACTCACAATAAGAGTGCTGGTTGTCTTGATTACCTATGCGCACTCATAGAGAAGTTTGGGAACGCAGTTCCAACAGTTTATCCTCTGGATCACTATGCTCGTCTTTCTATGGTTTCAACTCTTGATAGTTTGGGTATTGATCGACACTTCAGGGAGGAAATAAGGAGTGTACTGGATGAAACATACAGATGCTGGCTGCAGGGGGATGAAGATGTATTTTCAGATGCTGCCACCTGTGCTATGGCATTTCGACTCTTACGTGTTAACGGATATGATGTCTCTGCAGATCGGTTAAATCAATTTTCAGAAGACCATTTCTTTGATTCCCTTGGAGGATATCTAAGGGACACTGATGCTGCATTAAAACTGTTTAGGGCATCGGAAGTCATCATACATCCAGATGAATCAGTTCTAGAGAAACAAAATTACTGGACAAGGAATTTCCTAAAACAGGAATTTTCTAATAATTTAACTCAGCCTCACAGAATCAATAAGCATATTGGCCAAGAGGTGGATGATGCTCTTAAGTTTCCTTCCTATGCTACTTTGGGTCGGTTATCGAGCAGGAGGGCAATGATATCTTACAACACCAACAGTACAAGGATTTTAAAATCTTCTTATCGTTGTTTGAATATTGGGAATGAAGACTTCCTAAAACTGGCAGTGGAAGACTTCAATACGTGCCAATCGATACATCGGGAAGAACTCGAGCATCTTTCAAGGTGGGTTATCGAGAGCAGATTAGACAAGCTAAAATTTGCCAGGCAGAAGCTGGCTTACTGTTATTTCACTGCTGCTGGAACCCTTTTCCCTCCTGAACTAGCAGATGCTCGCATATCATGGGCCAAAAACGGAGTACTTACAACTGTGGTCGATGATTTCTTTGACGTTGGAGGTTCCGAAGAGGAACTAGTAAACCTTATACAATTGGTTGAGAAGTGGGATGTGAATCCAAGAACCGATTCTTGCTCCGAGAATGTTGAAATCGTTTTTTCAGCAATTAAGAGCTTAGTTAGTGAGATTGGCATCAATGCATTCGCAAGGCAAGAGCGCAGTGTGACAAATCACGTCATTGAGATTTGGTTGGATTTGCTCAAGGCTATGCTGAAGGAAGCTAAGTGGTTGATAAACAAGTCGGTGCCAACGATGGAGGAATACATGGAAAATGCGTACGTCACATTTGCCTTGGGACCTACTGTCCTTTCAAGTCTCTATTTGGTAGGCCCTAAGCTCTCAGAGGATGCTGTACGGAGTTTGGAATACTACCATCTGTATAGACTAATGAGCACTTTTGGGCGTCTTCTCAATGATATGCAAAGCTATAAGAGGGAAGCTGCGGAAGGGAAGCTAAATGCTGTAACATTGGCCGTGATTCATGGCAATGGTAGTGTTAGCGAAGAAGAGGCCATCAATGAGATGAAGAGCATTATCACCAGTAAGAGGAGAGAACTGCAAAGACTAGTTTTGCAGGACAAGGATAGTGTAGTTCCAAGAGCTTGCAAGGATCTGTTTTGGAACATGGGCAATATTTTGCATCTATTTTATGAAAGGCACGATGGATACACCGCGCATGACATGATGAAAACTGTAAATGAAGTAACCGAAGAACCCATCATTCTCAACGAATTGCAAAGCAGAAAGAAAATGAATGGCATGTACAGCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006629 GO:0006720 GO:0006721 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009685 GO:0009686 GO:0009719 GO:0009725 GO:0009739 GO:0009740 GO:0009755 GO:0009899 GO:0009987 GO:0010033 GO:0010333 GO:0010476 GO:0010817 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016829 GO:0016835 GO:0016838 GO:0019752 GO:0023052 GO:0032870 GO:0033993 GO:0042221 GO:0042445 GO:0042446 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0065008 GO:0070887 GO:0071229 GO:0071310 GO:0071370 GO:0071396 GO:0071495 GO:0071704 GO:1901576 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko00904 ko01100 ko01110 map00904 map01100 map01110

Pfam Domains

Protein Families

Terpene_synth Terpene_synth_C

Protein Analysis

792

Amino Acids

90.04

Weight (kDa)

5.84

Isoelectric Point (pI)

53.64

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Terpene_synth	PF01397	213 - 412	3e-42	Terpene synthase, N-terminal domain
Terpene_synth_C	PF03936	485 - 720	1.2e-69	Terpene synthase family, metal binding domain
Terpene_syn_C_2	PF19086	523 - 720	3.1e-44	Terpene synthase family 2, C-terminal metal binding

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0001708)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G79460
fragaria_vesca	FvH4_3g14116 FvH4_3g14130 FvH4_3g14131 FvH4_3g16270
malus_domestica	MD03G1187200.v1.1 MD10G1215700.v1.1 MD10G1215900.v1.1 MD10G1216200.v1.1
prunus_persica	Prupe.4G128500_v2.0.a1 Prupe.4G128500_v2.0.a1 Prupe.4G128500_v2.0.a1 Prupe.4G128600_v2.0.a1 Prupe.4G128700_v2.0.a1
pyrus_communis	pycom03g13990 pycom10g18220
rosa_chinensis	RchiOBHm_Chr5g0023441 RchiOBHm_Chr5g0023471 RchiOBHm_Chr5g0023641
rosa_laevigata	RLG00000032721
rosa_multiflora	Rmu_co8406553.1_g000001 Rmu_sc0004593.1_g000004 Rmu_sc0005664.1_g000001 Rmu_sc0005664.1_g000005
rosa_roxburghii	Rroxscaffold_1G00055600 Rroxscaffold_1G00055620 Rroxscaffold_1G00055910
rosa_rugosa	Rorug05G0079200 Rorug05G0079300
rosa_samantha	Rh5AG166100 Rh5AG166200 Rh5AG166300 Rh5AG167600 Rh5AG168000 Rh5BG165300 Rh5BG165500 Rh5CG180300 Rh5CG180400 Rh5CG181400 Rh5DG166800 Rh5DG166900 Rh5DG167800
rosa_wichuraiana	Rw0G019600 Rw5G014950 Rw5G014970 Rw5G015110 Rw5G015120 Rw5G015210

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	754
AccB1I	GGYRCC	1 cut(s)	1852
AccB7I	CCANNNNNTGG	1 cut(s)	1225
AccII	CGCG	1 cut(s)	2282
AciI	CCGC	2 cut(s)	2051, 2280
AclWI	GGATC	3 cut(s)	523, 810, 2226
AcoI	YGGCCR	2 cut(s)	1216, 2079
AcsI	RAATTY	5 cut(s)	97, 199, 1153, 1169, 1469
AcuI	CTGAAG	3 cut(s)	396, 847, 1841
AcyI	GRCGYC	1 cut(s)	2011
AfaI	GTAC	6 cut(s)	885, 1312, 1572, 1886, 1962, 2372
AfiI	CCNNNNNNNGG	5 cut(s)	512, 1165, 1225, 1372, 2154
AflII	CTTAAG	1 cut(s)	1238
AflIII	ACRYGT	1 cut(s)	973
AhdI	GACNNNNNGTC	1 cut(s)	1910
AhlI	ACTAGT	2 cut(s)	1624, 2173
AjiI	CACGTC	1 cut(s)	1783
AjnI	CCWGG	2 cut(s)	507, 1475
AjuI	GAANNNNNNNTTGG	2 cut(s)	1660, 1692
Alw21I	GWGCWC	1 cut(s)	2003
Alw26I	GTCTC	3 cut(s)	540, 997, 1928
AlwI	GGATC	3 cut(s)	523, 810, 2226
AlwNI	CAGNNNCTG	4 cut(s)	419, 906, 941, 1486
Ama87I	CYCGRG	1 cut(s)	1421
AoxI	GGCC	5 cut(s)	1216, 1557, 1936, 2079, 2118
ApeKI	GCWGC	6 cut(s)	430, 910, 941, 1070, 1508, 2048
ApoI	RAATTY	5 cut(s)	97, 199, 1153, 1169, 1469
ArsI	GACNNNNNNTTYG	2 cut(s)	1016, 1048
Asp700I	GAANNNNTTC	3 cut(s)	131, 1122, 1675
AspLEI	GCGC	3 cut(s)	755, 1767, 2284
AspS9I	GGNCC	5 cut(s)	187, 275, 1557, 1904, 1937
AsuHPI	GGTGA	3 cut(s)	374, 681, 2137
AvaI	CYCGRG	1 cut(s)	1421
AvaII	GGWCC	3 cut(s)	187, 275, 1904
BalI	TGGCCA	1 cut(s)	1218
BanI	GGYRCC	1 cut(s)	1852
BarI	GAAGNNNNNNTAC	4 cut(s)	1089, 1121, 1475, 1507
BauI	CACGAG	1 cut(s)	69
BbsI	GAAGAC	4 cut(s)	1029, 1365, 1389, 2006
Bbv12I	GWGCWC	1 cut(s)	2003
BbvI	GCAGC	6 cut(s)	442, 897, 928, 1057, 1495, 2035
BccI	CCATC	9 cut(s)	257, 442, 640, 700, 1855, 1989, 2129, 2264, 2333
BceAI	ACGGC	1 cut(s)	2066
BciT130I	CCWGG	2 cut(s)	509, 1477
BciVI	GTATCC	2 cut(s)	344, 2267
BcoDI	GTCTC	3 cut(s)	540, 997, 1928
BcuI	ACTAGT	2 cut(s)	1624, 2173
BfaI	CTAG	5 cut(s)	1127, 1535, 1625, 2174, 2377
BfmI	CTRYAG	2 cut(s)	911, 996
BfrI	CTTAAG	1 cut(s)	1238
BfuI	GTATCC	2 cut(s)	344, 2267
BglII	AGATCT	1 cut(s)	58
BisI	GCNGC	6 cut(s)	431, 911, 942, 1071, 1509, 2049
BlsI	GCNGC	6 cut(s)	432, 912, 943, 1072, 1510, 2050
BmcAI	AGTACT	1 cut(s)	1572
Bme1390I	CCNGG	2 cut(s)	509, 1477
Bme18I	GGWCC	3 cut(s)	187, 275, 1904
BmeRI	GACNNNNNGTC	1 cut(s)	1910
BmeT110I	CYCGRG	1 cut(s)	1421
BmgBI	CACGTC	1 cut(s)	1783
BmgT120I	GGNCC	5 cut(s)	187, 275, 1557, 1904, 1937
BmiI	GGNNCC	7 cut(s)	189, 213, 276, 1516, 1612, 1854, 1905
BmrFI	CCNGG	2 cut(s)	509, 1477
BpiI	GAAGAC	4 cut(s)	1029, 1365, 1389, 2006
Bpu10I	CCTNAGC	1 cut(s)	1941
BpuEI	CTTGAG	1 cut(s)	1793
Bsa29I	ATCGAT	1 cut(s)	1403
BsaAI	YACGTR	2 cut(s)	974, 1395
BsaBI	GATNNNNATC	2 cut(s)	1662, 1739
BsaHI	GRCGYC	1 cut(s)	2011
BsaJI	CCNNGG	5 cut(s)	324, 507, 508, 1042, 1899
BsaXI	ACNNNNNCTCC	2 cut(s)	346, 376
Bsc4I	CCNNNNNNNGG	5 cut(s)	512, 1165, 1225, 1372, 2154
Bse1I	ACTGG	5 cut(s)	644, 891, 1148, 1377, 2148
Bse3DI	GCAATG	5 cut(s)	189, 356, 489, 1293, 2101
Bse8I	GATNNNNATC	2 cut(s)	1662, 1739
BseBI	CCWGG	2 cut(s)	509, 1477
BseCI	ATCGAT	1 cut(s)	1403
BseDI	CCNNGG	5 cut(s)	324, 507, 508, 1042, 1899
BseGI	GGATG	9 cut(s)	289, 317, 390, 895, 925, 1108, 1234, 1663, 1960
BseJI	GATNNNNATC	2 cut(s)	1662, 1739
BseLI	CCNNNNNNNGG	5 cut(s)	512, 1165, 1225, 1372, 2154
BseMI	GCAATG	5 cut(s)	189, 356, 489, 1293, 2101
BseMII	CTCAG	3 cut(s)	594, 1202, 1962
BseNI	ACTGG	5 cut(s)	644, 891, 1148, 1377, 2148
BseRI	GAGGAG	1 cut(s)	2170
BseXI	GCAGC	6 cut(s)	442, 897, 928, 1057, 1495, 2035
Bsh1236I	CGCG	1 cut(s)	2282
BshFI	GGCC	5 cut(s)	1218, 1559, 1938, 2081, 2120
BshNI	GGYRCC	1 cut(s)	1852
BshVI	ATCGAT	1 cut(s)	1403
BsiHKAI	GWGCWC	1 cut(s)	2003
BsiHKCI	CYCGRG	1 cut(s)	1421
BsiWI	CGTACG	1 cut(s)	1884
BslFI	GGGAC	3 cut(s)	173, 1073, 1917
BslI	CCNNNNNNNGG	5 cut(s)	512, 1165, 1225, 1372, 2154
BsmAI	GTCTC	3 cut(s)	540, 997, 1928
BsmFI	GGGAC	3 cut(s)	173, 1073, 1917
BsmI	GAATGC	2 cut(s)	233, 1748
BsnI	GGCC	5 cut(s)	1218, 1559, 1938, 2081, 2120
BsoBI	CYCGRG	1 cut(s)	1421
Bsp1286I	GDGCHC	1 cut(s)	2003
Bsp1407I	TGTACA	1 cut(s)	2370
Bsp143I	GATC	7 cut(s)	58, 145, 515, 802, 853, 1000, 2218
BspACI	CCGC	2 cut(s)	2051, 2280
BspANI	GGCC	5 cut(s)	1218, 1559, 1938, 2081, 2120
BspCNI	CTCAG	3 cut(s)	593, 1201, 1961
BspDI	ATCGAT	1 cut(s)	1403
BspFNI	CGCG	1 cut(s)	2282
BspHI	TCATGA	1 cut(s)	651
BspLI	GGNNCC	7 cut(s)	189, 213, 276, 1516, 1612, 1854, 1905
BspMAI	CTGCAG	2 cut(s)	915, 1000
BspPI	GGATC	3 cut(s)	523, 810, 2226
BspQI	GCTCTTC	2 cut(s)	420, 2129
BspT107I	GGYRCC	1 cut(s)	1852
BspTI	CTTAAG	1 cut(s)	1238
BsrDI	GCAATG	5 cut(s)	189, 356, 489, 1293, 2101
BsrGI	TGTACA	1 cut(s)	2370
BsrI	ACTGG	5 cut(s)	644, 891, 1148, 1377, 2148
BssECI	CCNNGG	5 cut(s)	324, 507, 508, 1042, 1899
BssMI	GATC	7 cut(s)	58, 145, 515, 802, 853, 1000, 2218
BssNI	GRCGYC	1 cut(s)	2011
BssSI	CACGAG	1 cut(s)	69
BssT1I	CCWWGG	3 cut(s)	324, 1042, 1899
Bst2BI	CACGAG	1 cut(s)	69
Bst2UI	CCWGG	2 cut(s)	509, 1477
Bst4CI	ACNGT	7 cut(s)	790, 1083, 1310, 1496, 1582, 1912, 2302
Bst6I	CTCTTC	4 cut(s)	420, 1611, 2109, 2129
BstACI	GRCGYC	1 cut(s)	2011
BstAFI	CTTAAG	1 cut(s)	1238
BstAUI	TGTACA	1 cut(s)	2370
BstBAI	YACGTR	2 cut(s)	974, 1395
BstC8I	GCNNGC	4 cut(s)	908, 1488, 1546, 2212
BstDEI	CTNAG	8 cut(s)	406, 580, 1055, 1188, 1723, 1830, 1941, 1948
BstENI	CCTNNNNNAGG	1 cut(s)	1163
BstF5I	GGATG	9 cut(s)	289, 317, 390, 895, 925, 1108, 1234, 1663, 1960
BstFNI	CGCG	1 cut(s)	2282
BstHHI	GCGC	3 cut(s)	755, 1767, 2284
BstKTI	GATC	7 cut(s)	61, 148, 518, 805, 856, 1003, 2221
BstMAI	GTCTC	3 cut(s)	540, 997, 1928
BstMBI	GATC	7 cut(s)	58, 145, 515, 802, 853, 1000, 2218
BstMWI	GCNNNNNNNGC	6 cut(s)	179, 436, 573, 602, 950, 2373
BstNI	CCWGG	2 cut(s)	509, 1477
BstNSI	RCATGY	2 cut(s)	333, 2371
BstSCI	CCNGG	2 cut(s)	507, 1475
BstSFI	CTRYAG	2 cut(s)	911, 996
BstUI	CGCG	1 cut(s)	2282
BstV1I	GCAGC	6 cut(s)	442, 897, 928, 1057, 1495, 2035
BstV2I	GAAGAC	4 cut(s)	1029, 1365, 1389, 2006
BstX2I	RGATCY	2 cut(s)	58, 2218
BstYI	RGATCY	2 cut(s)	58, 2218
Bsu15I	ATCGAT	1 cut(s)	1403
BsuI	GTATCC	2 cut(s)	344, 2267
BsuRI	GGCC	5 cut(s)	1218, 1559, 1938, 2081, 2120
BsuTUI	ATCGAT	1 cut(s)	1403
BtgZI	GCGATG	1 cut(s)	420
BtrI	CACGTC	1 cut(s)	1783
BtsCI	GGATG	9 cut(s)	289, 317, 390, 895, 925, 1108, 1234, 1663, 1960
BtsI	GCAGTG	4 cut(s)	168, 1383, 1503, 1774
BtsIMutI	CAGTG	5 cut(s)	168, 1062, 1383, 1503, 1774
Cac8I	GCNNGC	4 cut(s)	908, 1488, 1546, 2212
CaiI	CAGNNNCTG	4 cut(s)	419, 906, 941, 1486
CciI	TCATGA	1 cut(s)	651
CfoI	GCGC	3 cut(s)	755, 1767, 2284
Cfr13I	GGNCC	5 cut(s)	187, 275, 1557, 1904, 1937
ClaI	ATCGAT	1 cut(s)	1403
CseI	GACGC	1 cut(s)	2000
Csp6I	GTAC	6 cut(s)	884, 1311, 1571, 1885, 1961, 2371
CviQI	GTAC	6 cut(s)	884, 1311, 1571, 1885, 1961, 2371
DdeI	CTNAG	8 cut(s)	406, 580, 1055, 1188, 1723, 1830, 1941, 1948
DpnI	GATC	7 cut(s)	60, 147, 517, 804, 855, 1002, 2220
DpnII	GATC	7 cut(s)	58, 145, 515, 802, 853, 1000, 2218
DraI	TTTAAA	1 cut(s)	1323
DriI	GACNNNNNGTC	1 cut(s)	1910
EaeI	YGGCCR	2 cut(s)	1216, 2079
Eam1104I	CTCTTC	4 cut(s)	420, 1611, 2109, 2129
Eam1105I	GACNNNNNGTC	1 cut(s)	1910
EarI	CTCTTC	4 cut(s)	420, 1611, 2109, 2129
Eco130I	CCWWGG	3 cut(s)	324, 1042, 1899
Eco32I	GATATC	2 cut(s)	1052, 1293
Eco47I	GGWCC	3 cut(s)	187, 275, 1904
Eco57I	CTGAAG	3 cut(s)	396, 847, 1841
Eco88I	CYCGRG	1 cut(s)	1421
EcoNI	CCTNNNNNAGG	1 cut(s)	1163
EcoO109I	RGGNCCY	3 cut(s)	275, 1904, 1937
EcoRII	CCWGG	2 cut(s)	507, 1475
EcoRV	GATATC	2 cut(s)	1052, 1293
EcoT14I	CCWWGG	3 cut(s)	324, 1042, 1899
EcoT22I	ATGCAT	2 cut(s)	390, 1750
ErhI	CCWWGG	3 cut(s)	324, 1042, 1899
FalI	AAGNNNNNCTT	2 cut(s)	297, 329
FaqI	GGGAC	3 cut(s)	173, 1073, 1917
FauNDI	CATATG	1 cut(s)	163
Fnu4HI	GCNGC	6 cut(s)	431, 911, 942, 1071, 1509, 2049
FokI	GGATG	9 cut(s)	276, 304, 397, 902, 932, 1095, 1241, 1670, 1967
Fsp4HI	GCNGC	6 cut(s)	431, 911, 942, 1071, 1509, 2049
FspAI	RTGCGCAY	1 cut(s)	754
FspBI	CTAG	5 cut(s)	1127, 1535, 1625, 2174, 2377
FspI	TGCGCA	1 cut(s)	754
GlaI	GCGC	3 cut(s)	754, 1766, 2283
GluI	GCNGC	6 cut(s)	431, 911, 942, 1071, 1509, 2049
HaeIII	GGCC	5 cut(s)	1218, 1559, 1938, 2081, 2120
HgaI	GACGC	1 cut(s)	2000
HhaI	GCGC	3 cut(s)	755, 1767, 2284
Hin1I	GRCGYC	1 cut(s)	2011
Hin6I	GCGC	3 cut(s)	753, 1765, 2282
HinP1I	GCGC	3 cut(s)	753, 1765, 2282
HincII	GTYRAC	2 cut(s)	300, 979
HindII	GTYRAC	2 cut(s)	300, 979
HpaI	GTTAAC	2 cut(s)	300, 979
HphI	GGTGA	3 cut(s)	374, 681, 2137
Hpy166II	GTNNAC	4 cut(s)	300, 884, 979, 1630
Hpy188I	TCNGA	9 cut(s)	205, 583, 616, 937, 1021, 1096, 1615, 1687, 1951
Hpy8I	GTNNAC	4 cut(s)	300, 884, 979, 1630
HpyAV	CCTTC	7 cut(s)	16, 51, 201, 611, 1257, 1816, 2048
HpyCH4III	ACNGT	7 cut(s)	790, 1083, 1310, 1496, 1582, 1912, 2302
HpyCH4IV	ACGT	5 cut(s)	973, 1394, 1602, 1782, 1887
HpyF10VI	GCNNNNNNNGC	6 cut(s)	179, 436, 573, 602, 950, 2373
HpyF3I	CTNAG	8 cut(s)	406, 580, 1055, 1188, 1723, 1830, 1941, 1948
HpySE526I	ACGT	5 cut(s)	973, 1394, 1602, 1782, 1887
Hsp92I	GRCGYC	1 cut(s)	2011
HspAI	GCGC	3 cut(s)	753, 1765, 2282
KspAI	GTTAAC	2 cut(s)	300, 979
Kzo9I	GATC	7 cut(s)	58, 145, 515, 802, 853, 1000, 2218
LguI	GCTCTTC	2 cut(s)	420, 2129
LmnI	GCTCC	2 cut(s)	211, 1688
Lsp1109I	GCAGC	6 cut(s)	442, 897, 928, 1057, 1495, 2035
MaeI	CTAG	5 cut(s)	1127, 1535, 1625, 2174, 2377
MaeII	ACGT	5 cut(s)	973, 1394, 1602, 1782, 1887
MaeIII	GTNAC	5 cut(s)	630, 1771, 1888, 2071, 2311
MalI	GATC	7 cut(s)	60, 147, 517, 804, 855, 1002, 2220
MboI	GATC	7 cut(s)	58, 145, 515, 802, 853, 1000, 2218
MfeI	CAATTG	1 cut(s)	1640
MflI	RGATCY	2 cut(s)	58, 2218
MhlI	GDGCHC	1 cut(s)	2003
MlsI	TGGCCA	1 cut(s)	1218
MluNI	TGGCCA	1 cut(s)	1218
MlyI	GAGTC	2 cut(s)	407, 960
MmeI	TCCRAC	4 cut(s)	36, 809, 1585, 1779
Mox20I	TGGCCA	1 cut(s)	1218
Mph1103I	ATGCAT	2 cut(s)	390, 1750
MroXI	GAANNNNTTC	3 cut(s)	131, 1122, 1675
MscI	TGGCCA	1 cut(s)	1218
MslI	CAYNNNNRTG	3 cut(s)	901, 1113, 2094
Msp20I	TGGCCA	1 cut(s)	1218
MspCI	CTTAAG	1 cut(s)	1238
MspR9I	CCNGG	2 cut(s)	509, 1477
MunI	CAATTG	1 cut(s)	1640
Mva1269I	GAATGC	2 cut(s)	233, 1748
MvaI	CCWGG	2 cut(s)	509, 1477
MvnI	CGCG	1 cut(s)	2282
MwoI	GCNNNNNNNGC	6 cut(s)	179, 436, 573, 602, 950, 2373
NdeI	CATATG	1 cut(s)	163
NdeII	GATC	7 cut(s)	58, 145, 515, 802, 853, 1000, 2218
NlaIV	GGNNCC	7 cut(s)	189, 213, 276, 1516, 1612, 1854, 1905
NmuCI	GTSAC	3 cut(s)	630, 1771, 1888
NsbI	TGCGCA	1 cut(s)	754
NsiI	ATGCAT	2 cut(s)	390, 1750
NspI	RCATGY	2 cut(s)	333, 2371
PaeR7I	CTCGAG	1 cut(s)	1421
PagI	TCATGA	1 cut(s)	651
PasI	CCCWGGG	1 cut(s)	508
PciSI	GCTCTTC	2 cut(s)	420, 2129
PcsI	WCGNNNNNNNCGW	3 cut(s)	970, 1400, 1856
PctI	GAATGC	2 cut(s)	233, 1748
PdmI	GAANNNNTTC	3 cut(s)	131, 1122, 1675
PfeI	GAWTC	8 cut(s)	476, 499, 1037, 1118, 1200, 1663, 1676, 2086
Pfl23II	CGTACG	1 cut(s)	1884
PflMI	CCANNNNNTGG	1 cut(s)	1225
PkrI	GCNGC	6 cut(s)	432, 912, 943, 1072, 1510, 2050
PleI	GAGTC	2 cut(s)	406, 960
PpsI	GAGTC	2 cut(s)	406, 960
Ppu21I	YACGTR	2 cut(s)	974, 1395
PpuMI	RGGWCCY	2 cut(s)	275, 1904
Psp5II	RGGWCCY	2 cut(s)	275, 1904
Psp6I	CCWGG	2 cut(s)	507, 1475
PspGI	CCWGG	2 cut(s)	507, 1475
PspLI	CGTACG	1 cut(s)	1884
PspN4I	GGNNCC	7 cut(s)	189, 213, 276, 1516, 1612, 1854, 1905
PspPI	GGNCC	5 cut(s)	187, 275, 1557, 1904, 1937
PspPPI	RGGWCCY	2 cut(s)	275, 1904
PspXI	VCTCGAGB	1 cut(s)	1421
PstI	CTGCAG	2 cut(s)	915, 1000
PstNI	CAGNNNCTG	4 cut(s)	419, 906, 941, 1486
PsuI	RGATCY	2 cut(s)	58, 2218
RsaI	GTAC	6 cut(s)	885, 1312, 1572, 1886, 1962, 2372
RsaNI	GTAC	6 cut(s)	884, 1311, 1571, 1885, 1961, 2371
RseI	CAYNNNNRTG	3 cut(s)	901, 1113, 2094
SapI	GCTCTTC	2 cut(s)	420, 2129
SatI	GCNGC	6 cut(s)	431, 911, 942, 1071, 1509, 2049
Sau3AI	GATC	7 cut(s)	58, 145, 515, 802, 853, 1000, 2218
Sau96I	GGNCC	5 cut(s)	187, 275, 1557, 1904, 1937
ScaI	AGTACT	1 cut(s)	1572
SchI	GAGTC	2 cut(s)	407, 960
ScrFI	CCNGG	2 cut(s)	509, 1477
SduI	GDGCHC	1 cut(s)	2003
SfcI	CTRYAG	2 cut(s)	911, 996
Sfr274I	CTCGAG	1 cut(s)	1421
SinI	GGWCC	3 cut(s)	187, 275, 1904
SlaI	CTCGAG	1 cut(s)	1421
SmiMI	CAYNNNNRTG	3 cut(s)	901, 1113, 2094
SmlI	CTYRAG	3 cut(s)	1238, 1421, 1808
SmoI	CTYRAG	3 cut(s)	1238, 1421, 1808
SpeI	ACTAGT	2 cut(s)	1624, 2173
SsiI	CCGC	2 cut(s)	2051, 2280
SspI	AATATT	3 cut(s)	459, 1348, 2242
SspMI	CTAG	5 cut(s)	1127, 1535, 1625, 2174, 2377
StyD4I	CCNGG	2 cut(s)	507, 1475
StyI	CCWWGG	3 cut(s)	324, 1042, 1899
TaaI	ACNGT	7 cut(s)	790, 1083, 1310, 1496, 1582, 1912, 2302
TaiI	ACGT	5 cut(s)	976, 1397, 1605, 1785, 1890
TaqI	TCGA	7 cut(s)	856, 964, 1274, 1403, 1422, 1446, 1587
TatI	WGTACW	4 cut(s)	883, 1310, 1570, 2370
TfiI	GAWTC	8 cut(s)	476, 499, 1037, 1118, 1200, 1663, 1676, 2086
TscAI	CASTG	5 cut(s)	175, 1069, 1383, 1510, 1774
TseFI	GTSAC	3 cut(s)	630, 1771, 1888
TseI	GCWGC	6 cut(s)	430, 910, 941, 1070, 1508, 2048
Tsp45I	GTSAC	3 cut(s)	630, 1771, 1888
TspGWI	ACGGA	3 cut(s)	996, 1581, 1978
TspRI	CASTG	5 cut(s)	175, 1069, 1383, 1510, 1774
Van91I	CCANNNNNTGG	1 cut(s)	1225
Vha464I	CTTAAG	1 cut(s)	1238
VpaK11BI	GGWCC	3 cut(s)	187, 275, 1904
XagI	CCTNNNNNAGG	1 cut(s)	1163
XapI	RAATTY	5 cut(s)	97, 199, 1153, 1169, 1469
XbaI	TCTAGA	1 cut(s)	1126
XceI	RCATGY	2 cut(s)	333, 2371
XcmI	CCANNNNNNNNNTGG	3 cut(s)	668, 952, 1483
XhoI	CTCGAG	1 cut(s)	1421
XmnI	GAANNNNTTC	3 cut(s)	131, 1122, 1675
XspI	CTAG	5 cut(s)	1127, 1535, 1625, 2174, 2377
ZrmI	AGTACT	1 cut(s)	1572
Zsp2I	ATGCAT	2 cut(s)	390, 1750

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.