Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr7

Orthogroup ID

OG0001708

Physical Location & Seq

Genomic Coordinates Forward (+)

18197503 .. 18205841

Gene Structure 8339 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000032721

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2676 bp

ATGCATAAGGATAAGATTTTCAAGCTCGCCAAGGGCTTCAGGGGAAGGGCCAAGAACTGCATAAGGATAGCCAGAGAGAGGGTGGAGAAGGCCTTGCAGTATTCCTACAGAGATCGTCGCACCAAGAAGAGGGACATGCGTGCGCTGTGGATCCAACGTATCAATGCTGGCACCCGCATTCATGGTGTTAATTATGGGAATTTCATGCACGGATTGATGAAGGAGAACATTCAGCTAAACAGGAAAGTTTTATCAGAGCTTTCTATGCATGAACCATACACTTTCAAGGCCCTCGTAGATGTCTCCCATAGCGCATTCCCTGGGAACAAGGCGAGTCAAGAGATTCAAAGCTTTCTGTCTAAACCATGTCCTTCTCCCAGCTCAACACTTTCAGGTGTTGCACCTTTTCCTCATCAGAGTCTGGAAGGAACTAAACAAAGAATAAAGAAGATGTTCAACAAGGTTGATCTCTCTGTTTCATCATATGACACTGCTTGGGTGGCAATGGTCCCTTCTCCAAATTCCGGGAAGGAGCCTTTCTTCCCTGAATGCGTAAATTGGTTGTTAGATAATCAACTTCATGATGGCTCATGGGGTCCTCCTAATCTGCATCAGTTGTTAACAAAAGATGCCCTCTTATCCACGTTAGCATGCATCCTTGCACTAAGGCGATGGAATATCGGTGAAGAACTAATTGACAAGGGTCTGCATTTTATCGAGTCAAATTTAGCTTCAGCTACTGATGGAGAGCAACCATCTCCTGTTGGGTTTGATATAATATTTCCTTCCATGATTGAATCTGCAATGAATTTGGATGTGAATCTACCTTTGGGAGGATTAACCTTAGATGCTTTACTTCTCAGGAGAGACTTTGAGCTTCAAAGAGGCAACAGAAGCAACTCAGAGGGTGGGAGAGCCTATCTCGCATATATTTCAGAAGGAATCGGAAAGTCACAGGACTGGGAGATGGTCATGAAGTACCAGAAGAAGAATGGGTCCTTGTTTAACTCACCATCAACTACTGCTGCTGCTTTTACTCACCTCAACAATGCTGGTTGTCTTAGTTATCTACGCTCACTCATAGAAAAGTTTGGAAGCGCAGTTCCAACGATTTATCCTCTAGATAACTATGCTCGCCTTTCTATGGTTTCCAGTCTTGAAAGTATGGGCGTTGATCGACATTTCAGGGAGGAAATAAGGAGTGTACTCGATGAAACATACAGATGCTGGCTGCAGGGGGATGAAGATATATTTTCAGATGCTGGCACCTGTGCTATGGCATTTCGACTCTTACGTATTCATGGATATGATGTTTCTGCAGATCCATTAAGTCAATTTTCAGAAGACAGTTTCTTCAATTCCCTTGGAGGATATCTGAAGGACACTGGTGCTGCCTTAGAACTTTTTAGGGCTTCAGAAGTCATCATTCATCCAGATGAATCAGTTCTAGAGAAACAAAATTACTGGACAAGTCATTTCTTGAAACAGGAATTATCAAATAACTTAACTCAGGCTCATAGACTCAATAAGCATATTGATCAGGTGGATGATGCTCTTCGGTTTCCTTCCTATGCAACTCTGGGTCGGTTATCAAGCAGGAAAGCAATAAAATATTACAACACTGATAGTACAAAGATTTTAAAATCTTCTTATCGTTGTTCAAATGTTGGGAATGAAGACTTCCTAAAACTGGCAGTGGAAGACTTTAATATGTGTCAATCGATACATCGGGAAGAACTCGAGTATCTTTCAAGGTGGGTTGTCAAGAGCAGATTAGACGAGCTAAAATTTGCTAGGCAGAAGCAGGCGTACTGTTACTTCTCTGCTGCTGCAACCCTTTTCCCTCCTGAACTATCAGATGCCCGCATATCATGGGCCAAAAACGGAGTGCTTACAACTGTGGTTGATGATTTCTTTGACGTTGGAGGTTCTGAAGAGGAACTAGTAAACCTTATACAATTGATCGAGAAGTGGGATGTGAATGCGAAAACTGATTCTTGTTCTGAGCAAGTTGAAATCATATTTTCAGCAATTAAGAGCACAATTAATGAGATTGGAGCCAATGCATTCCCACGGCAACAGCGCAGTGTAACAAATCACGTCATAGAGATTTGGTTGGATTTGGTTAAGTCTATGCTGAAGGAAGCTCAGTGGTTGATAAACAATTCGGCGCCGGCACTGGATGAATACATGGAAAATGCACATGTATCATTTGCCTTGGGACCGATTGTGCTTCCAGCTCTCTATTTGGTAGGGCCTAAGCTCTCAGAGGAGGCTGTACGGAGTTTCGAATTCCACCAGTTGTATAAACTTATGAGCACTGGTGGGCGTCTTCTCAATGATATGCAAACCTTTAAGAGGGAATCTGCGGAAGGGAAGCTAAATACTGTTACATTGGCCATGCTTCATGGCAATGGCAGTGCTACTGAAGAAGAGACCTTCAGTGAGATGGAGAGCATTATTGTCATTAAGAGGAGAGAATTGCAAAGACTAGTTTTGCAGCAGGATAGCTTAATTCCAAGAGCTTGCAAGGACCTGTTTTGGAACATGTGCAAAATTGTGCACCTATTTTATGCAAAGCACGATGGATTCACTGGGCATGACTTGATGAAAACCGTAAATGGACTAACCGAAGAACCGATCATTCTCAGTGAATTGCAAGTAGAAAGTAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006629 GO:0006720 GO:0006721 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009685 GO:0009686 GO:0009719 GO:0009725 GO:0009739 GO:0009740 GO:0009755 GO:0009899 GO:0009987 GO:0010033 GO:0010333 GO:0010476 GO:0010817 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016829 GO:0016835 GO:0016838 GO:0019752 GO:0023052 GO:0032870 GO:0033993 GO:0042221 GO:0042445 GO:0042446 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0065008 GO:0070887 GO:0071229 GO:0071310 GO:0071370 GO:0071396 GO:0071495 GO:0071704 GO:1901576 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko00904 ko01100 ko01110 map00904 map01100 map01110

Pfam Domains

Protein Families

Terpene_synth Terpene_synth_C

Protein Analysis

892

Amino Acids

100.92

Weight (kDa)

6.35

Isoelectric Point (pI)

50.92

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ribosomal_L20	PF00453	3 - 98	1.3e-39	Ribosomal protein L20
Terpene_synth	PF01397	320 - 514	2.5e-42	Terpene synthase, N-terminal domain
Terpene_synth_C	PF03936	592 - 826	6.5e-68	Terpene synthase family, metal binding domain
Terpene_syn_C_2	PF19086	629 - 821	6.5e-40	Terpene synthase family 2, C-terminal metal binding

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0001708)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G79460
fragaria_vesca	FvH4_3g14116 FvH4_3g14130 FvH4_3g14131 FvH4_3g16270
malus_domestica	MD03G1187200.v1.1 MD10G1215700.v1.1 MD10G1215900.v1.1 MD10G1216200.v1.1
prunus_persica	Prupe.4G128500_v2.0.a1 Prupe.4G128500_v2.0.a1 Prupe.4G128500_v2.0.a1 Prupe.4G128600_v2.0.a1 Prupe.4G128700_v2.0.a1
pyrus_communis	pycom03g13990 pycom10g18220
rosa_chinensis	RchiOBHm_Chr5g0023441 RchiOBHm_Chr5g0023471 RchiOBHm_Chr5g0023641
rosa_laevigata	RLG00000032721
rosa_multiflora	Rmu_co8406553.1_g000001 Rmu_sc0004593.1_g000004 Rmu_sc0005664.1_g000001 Rmu_sc0005664.1_g000005
rosa_roxburghii	Rroxscaffold_1G00055600 Rroxscaffold_1G00055620 Rroxscaffold_1G00055910
rosa_rugosa	Rorug05G0079200 Rorug05G0079300
rosa_samantha	Rh5AG166100 Rh5AG166200 Rh5AG166300 Rh5AG167600 Rh5AG168000 Rh5BG165300 Rh5BG165500 Rh5CG180300 Rh5CG180400 Rh5CG181400 Rh5DG166800 Rh5DG166900 Rh5DG167800
rosa_wichuraiana	Rw0G019600 Rw5G014950 Rw5G014970 Rw5G015110 Rw5G015120 Rw5G015210

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	3 cut(s)	170, 1265, 2170
AciI	CCGC	3 cut(s)	175, 1864, 2369
AclWI	GGATC	3 cut(s)	145, 158, 1316
AcoI	YGGCCR	1 cut(s)	2397
AcsI	RAATTY	6 cut(s)	199, 520, 724, 808, 1787, 2291
AcuI	CTGAAG	8 cut(s)	22, 717, 1397, 1398, 1953, 2159, 2425, 2448
AcyI	GRCGYC	2 cut(s)	2171, 2329
AfaI	GTAC	5 cut(s)	980, 1206, 1630, 1811, 2280
AfiI	CCNNNNNNNGG	7 cut(s)	78, 129, 524, 833, 1144, 1690, 2358
AflIII	ACRYGT	2 cut(s)	2203, 2547
AhlI	ACTAGT	2 cut(s)	1942, 2491
AjiI	CACGTC	1 cut(s)	2101
AjnI	CCWGG	1 cut(s)	319
AjuI	GAANNNNNNNTTGG	2 cut(s)	812, 844
AloI	GAACNNNNNNTCC	2 cut(s)	1086, 1118
Alw21I	GWGCWC	3 cut(s)	2042, 2321, 2565
Alw26I	GTCTC	3 cut(s)	307, 861, 2429
Alw44I	GTGCAC	1 cut(s)	2561
AlwI	GGATC	3 cut(s)	145, 158, 1316
AlwNI	CAGNNNCTG	4 cut(s)	421, 740, 1227, 1262
Ama87I	CYCGRG	1 cut(s)	1739
AoxI	GGCC	6 cut(s)	48, 90, 288, 1875, 2255, 2397
ApaLI	GTGCAC	1 cut(s)	2561
ApeKI	GCWGC	7 cut(s)	1025, 1028, 1231, 1391, 1826, 1829, 2500
ApoI	RAATTY	6 cut(s)	199, 520, 724, 808, 1787, 2291
AseI	ATTAAT	1 cut(s)	2046
Asp700I	GAANNNNTTC	2 cut(s)	452, 1443
AspLEI	GCGC	5 cut(s)	145, 314, 1100, 2085, 2173
AspS9I	GGNCC	9 cut(s)	48, 289, 508, 596, 996, 1875, 2222, 2255, 2533
AsuC2I	CCSGG	1 cut(s)	526
AsuHPI	GGTGA	3 cut(s)	695, 1002, 1031
AsuII	TTCGAA	1 cut(s)	2289
AvaI	CYCGRG	1 cut(s)	1739
AvaII	GGWCC	5 cut(s)	508, 596, 996, 2222, 2533
BaeGI	GKGCMC	1 cut(s)	2565
BaeI	ACNNNNGTAYC	2 cut(s)	1727, 1760
BalI	TGGCCA	1 cut(s)	2399
BamHI	GGATCC	1 cut(s)	150
BanI	GGYRCC	3 cut(s)	170, 1265, 2170
BarI	GAAGNNNNNNTAC	4 cut(s)	1793, 1802, 1825, 1834
BbsI	GAAGAC	4 cut(s)	1350, 1683, 1707, 2324
Bbv12I	GWGCWC	3 cut(s)	2042, 2321, 2565
BbvI	GCAGC	7 cut(s)	1012, 1015, 1218, 1378, 1813, 1816, 2512
BccI	CCATC	8 cut(s)	578, 666, 737, 763, 961, 1021, 2443, 2579
BceAI	ACGGC	1 cut(s)	2090
BciT130I	CCWGG	1 cut(s)	321
BclI	TGATCA	1 cut(s)	1537
BcnI	CCSGG	1 cut(s)	526
BcoDI	GTCTC	3 cut(s)	307, 861, 2429
BcuI	ACTAGT	2 cut(s)	1942, 2491
BfaI	CTAG	5 cut(s)	1121, 1448, 1794, 1943, 2492
BfmI	CTRYAG	3 cut(s)	106, 1232, 1317
BfoI	RGCGCY	1 cut(s)	2174
BisI	GCNGC	7 cut(s)	1026, 1029, 1232, 1392, 1827, 1830, 2501
BlsI	GCNGC	7 cut(s)	1027, 1030, 1233, 1393, 1828, 1831, 2502
Bme1390I	CCNGG	2 cut(s)	321, 526
Bme18I	GGWCC	5 cut(s)	508, 596, 996, 2222, 2533
BmeT110I	CYCGRG	1 cut(s)	1739
BmgBI	CACGTC	1 cut(s)	2101
BmgT120I	GGNCC	9 cut(s)	48, 289, 508, 596, 996, 1875, 2222, 2255, 2533
BmrFI	CCNGG	2 cut(s)	321, 526
BmrI	ACTGGG	2 cut(s)	970, 2604
BmsI	GCATC	8 cut(s)	619, 619, 663, 838, 1214, 1249, 1540, 1849
BmuI	ACTGGG	2 cut(s)	970, 2604
BoxI	GACNNNNGTC	1 cut(s)	702
BpiI	GAAGAC	4 cut(s)	1350, 1683, 1707, 2324
BplI	GAGNNNNNCTC	2 cut(s)	906, 938
Bpu10I	CCTNAGC	1 cut(s)	2259
Bpu14I	TTCGAA	1 cut(s)	2289
BpuMI	CCSGG	1 cut(s)	526
Bsa29I	ATCGAT	1 cut(s)	1721
BsaAI	YACGTR	1 cut(s)	1295
BsaBI	GATNNNNATC	1 cut(s)	819
BsaHI	GRCGYC	2 cut(s)	2171, 2329
BsaI	GGTCTC	1 cut(s)	2429
BsaJI	CCNNGG	6 cut(s)	30, 319, 320, 1363, 2072, 2217
Bsc4I	CCNNNNNNNGG	7 cut(s)	78, 129, 524, 833, 1144, 1690, 2358
Bse118I	RCCGGY	1 cut(s)	2173
Bse1I	ACTGG	9 cut(s)	965, 1152, 1390, 1469, 1695, 2184, 2299, 2326, 2599
Bse3DI	GCAATG	3 cut(s)	510, 810, 2419
Bse8I	GATNNNNATC	1 cut(s)	819
BseBI	CCWGG	1 cut(s)	321
BseCI	ATCGAT	1 cut(s)	1721
BseDI	CCNNGG	6 cut(s)	30, 319, 320, 1363, 2072, 2217
BseGI	GGATG	7 cut(s)	654, 820, 1246, 1429, 1552, 1981, 2188
BseJI	GATNNNNATC	1 cut(s)	819
BseLI	CCNNNNNNNGG	7 cut(s)	78, 129, 524, 833, 1144, 1690, 2358
BseMI	GCAATG	3 cut(s)	510, 810, 2419
BseMII	CTCAG	7 cut(s)	874, 915, 1523, 1995, 2162, 2280, 2662
BseNI	ACTGG	9 cut(s)	965, 1152, 1390, 1469, 1695, 2184, 2299, 2326, 2599
BseRI	GAGGAG	2 cut(s)	2285, 2488
BseSI	GKGCMC	1 cut(s)	2565
BseXI	GCAGC	7 cut(s)	1012, 1015, 1218, 1378, 1813, 1816, 2512
BseYI	CCCAGC	1 cut(s)	377
BshFI	GGCC	6 cut(s)	50, 92, 290, 1877, 2257, 2399
BshNI	GGYRCC	3 cut(s)	170, 1265, 2170
BshVI	ATCGAT	1 cut(s)	1721
BsiHKAI	GWGCWC	3 cut(s)	2042, 2321, 2565
BsiHKCI	CYCGRG	1 cut(s)	1739
BsiSI	CCGG	2 cut(s)	525, 2174
BslFI	GGGAC	3 cut(s)	146, 494, 2235
BslI	CCNNNNNNNGG	7 cut(s)	78, 129, 524, 833, 1144, 1690, 2358
BsmAI	GTCTC	3 cut(s)	307, 861, 2429
BsmFI	GGGAC	3 cut(s)	146, 494, 2235
BsmI	GAATGC	5 cut(s)	177, 314, 554, 1987, 2066
BsnI	GGCC	6 cut(s)	50, 92, 290, 1877, 2257, 2399
Bso31I	GGTCTC	1 cut(s)	2429
BsoBI	CYCGRG	1 cut(s)	1739
Bsp119I	TTCGAA	1 cut(s)	2289
Bsp1286I	GDGCHC	3 cut(s)	2042, 2321, 2565
Bsp143I	GATC	8 cut(s)	112, 150, 466, 1174, 1321, 1537, 1962, 2640
BspACI	CCGC	3 cut(s)	175, 1864, 2369
BspANI	GGCC	6 cut(s)	50, 92, 290, 1877, 2257, 2399
BspCNI	CTCAG	7 cut(s)	873, 914, 1522, 1996, 2161, 2279, 2661
BspDI	ATCGAT	1 cut(s)	1721
BspHI	TCATGA	2 cut(s)	580, 972
BspMAI	CTGCAG	2 cut(s)	1236, 1321
BspPI	GGATC	3 cut(s)	145, 158, 1316
BspQI	GCTCTTC	1 cut(s)	1560
BspT104I	TTCGAA	1 cut(s)	2289
BspT107I	GGYRCC	3 cut(s)	170, 1265, 2170
BspTNI	GGTCTC	1 cut(s)	2429
BsrDI	GCAATG	3 cut(s)	510, 810, 2419
BsrFI	RCCGGY	1 cut(s)	2173
BsrI	ACTGG	9 cut(s)	965, 1152, 1390, 1469, 1695, 2184, 2299, 2326, 2599
BssAI	RCCGGY	1 cut(s)	2173
BssECI	CCNNGG	6 cut(s)	30, 319, 320, 1363, 2072, 2217
BssMI	GATC	8 cut(s)	112, 150, 466, 1174, 1321, 1537, 1962, 2640
BssNI	GRCGYC	2 cut(s)	2171, 2329
BssT1I	CCWWGG	3 cut(s)	30, 1363, 2217
Bst2UI	CCWGG	1 cut(s)	321
Bst4CI	ACNGT	5 cut(s)	1349, 1814, 1900, 2389, 2617
Bst6I	CTCTTC	4 cut(s)	122, 1560, 1929, 2427
BstACI	GRCGYC	2 cut(s)	2171, 2329
BstBAI	YACGTR	1 cut(s)	1295
BstBI	TTCGAA	1 cut(s)	2289
BstDSI	CCRYGG	1 cut(s)	2072
BstENI	CCTNNNNNAGG	2 cut(s)	831, 2356
BstF5I	GGATG	7 cut(s)	654, 820, 1246, 1429, 1552, 1981, 2188
BstH2I	RGCGCY	1 cut(s)	2174
BstHHI	GCGC	5 cut(s)	145, 314, 1100, 2085, 2173
BstKTI	GATC	8 cut(s)	115, 153, 469, 1177, 1324, 1540, 1965, 2643
BstMAI	GTCTC	3 cut(s)	307, 861, 2429
BstMBI	GATC	8 cut(s)	112, 150, 466, 1174, 1321, 1537, 1962, 2640
BstMWI	GCNNNNNNNGC	4 cut(s)	265, 500, 894, 923
BstNI	CCWGG	1 cut(s)	321
BstNSI	RCATGY	4 cut(s)	139, 654, 2207, 2551
BstPAI	GACNNNNGTC	1 cut(s)	702
BstSCI	CCNGG	2 cut(s)	319, 524
BstSFI	CTRYAG	3 cut(s)	106, 1232, 1317
BstSLI	GKGCMC	1 cut(s)	2565
BstSNI	TACGTA	1 cut(s)	1295
BstV1I	GCAGC	7 cut(s)	1012, 1015, 1218, 1378, 1813, 1816, 2512
BstV2I	GAAGAC	4 cut(s)	1350, 1683, 1707, 2324
BstX2I	RGATCY	2 cut(s)	150, 1321
BstYI	RGATCY	2 cut(s)	150, 1321
Bsu15I	ATCGAT	1 cut(s)	1721
BsuRI	GGCC	6 cut(s)	50, 92, 290, 1877, 2257, 2399
BsuTUI	ATCGAT	1 cut(s)	1721
BtgI	CCRYGG	1 cut(s)	2072
BtgZI	GCGATG	1 cut(s)	685
BtrI	CACGTC	1 cut(s)	2101
BtsCI	GGATG	7 cut(s)	654, 820, 1246, 1429, 1552, 1981, 2188
BtsI	GCAGTG	4 cut(s)	489, 1701, 2092, 2425
CaiI	CAGNNNCTG	4 cut(s)	421, 740, 1227, 1262
CciI	TCATGA	2 cut(s)	580, 972
CfoI	GCGC	5 cut(s)	145, 314, 1100, 2085, 2173
Cfr10I	RCCGGY	1 cut(s)	2173
Cfr13I	GGNCC	9 cut(s)	48, 289, 508, 596, 996, 1875, 2222, 2255, 2533
ClaI	ATCGAT	1 cut(s)	1721
CseI	GACGC	1 cut(s)	2318
Csp6I	GTAC	5 cut(s)	979, 1205, 1629, 1810, 2279
CviQI	GTAC	5 cut(s)	979, 1205, 1629, 1810, 2279
DinI	GGCGCC	1 cut(s)	2172
DpnI	GATC	8 cut(s)	114, 152, 468, 1176, 1323, 1539, 1964, 2642
DpnII	GATC	8 cut(s)	112, 150, 466, 1174, 1321, 1537, 1962, 2640
DraI	TTTAAA	1 cut(s)	1641
EaeI	YGGCCR	1 cut(s)	2397
Eam1104I	CTCTTC	4 cut(s)	122, 1560, 1929, 2427
EarI	CTCTTC	4 cut(s)	122, 1560, 1929, 2427
Eco105I	TACGTA	1 cut(s)	1295
Eco130I	CCWWGG	3 cut(s)	30, 1363, 2217
Eco147I	AGGCCT	1 cut(s)	92
Eco31I	GGTCTC	1 cut(s)	2429
Eco32I	GATATC	1 cut(s)	1373
Eco47I	GGWCC	5 cut(s)	508, 596, 996, 2222, 2533
Eco57I	CTGAAG	8 cut(s)	22, 717, 1397, 1398, 1953, 2159, 2425, 2448
Eco88I	CYCGRG	1 cut(s)	1739
EcoNI	CCTNNNNNAGG	2 cut(s)	831, 2356
EcoO109I	RGGNCCY	5 cut(s)	289, 596, 996, 2255, 2533
EcoRI	GAATTC	1 cut(s)	2291
EcoRII	CCWGG	1 cut(s)	319
EcoRV	GATATC	1 cut(s)	1373
EcoT14I	CCWWGG	3 cut(s)	30, 1363, 2217
EcoT22I	ATGCAT	4 cut(s)	6, 270, 656, 2068
EgeI	GGCGCC	1 cut(s)	2172
EheI	GGCGCC	1 cut(s)	2172
ErhI	CCWWGG	3 cut(s)	30, 1363, 2217
FaqI	GGGAC	3 cut(s)	146, 494, 2235
FauI	CCCGC	2 cut(s)	182, 1871
FauNDI	CATATG	1 cut(s)	484
FbaI	TGATCA	1 cut(s)	1537
Fnu4HI	GCNGC	7 cut(s)	1026, 1029, 1232, 1392, 1827, 1830, 2501
FokI	GGATG	7 cut(s)	641, 827, 1253, 1416, 1559, 1988, 2195
Fsp4HI	GCNGC	7 cut(s)	1026, 1029, 1232, 1392, 1827, 1830, 2501
FspBI	CTAG	5 cut(s)	1121, 1448, 1794, 1943, 2492
GlaI	GCGC	5 cut(s)	144, 313, 1099, 2084, 2172
GluI	GCNGC	7 cut(s)	1026, 1029, 1232, 1392, 1827, 1830, 2501
GsaI	CCCAGC	1 cut(s)	381
HaeII	RGCGCY	1 cut(s)	2174
HaeIII	GGCC	6 cut(s)	50, 92, 290, 1877, 2257, 2399
HapII	CCGG	2 cut(s)	525, 2174
HgaI	GACGC	1 cut(s)	2318
HhaI	GCGC	5 cut(s)	145, 314, 1100, 2085, 2173
Hin1I	GRCGYC	2 cut(s)	2171, 2329
Hin6I	GCGC	5 cut(s)	143, 312, 1098, 2083, 2171
HinP1I	GCGC	5 cut(s)	143, 312, 1098, 2083, 2171
HincII	GTYRAC	1 cut(s)	621
HindII	GTYRAC	1 cut(s)	621
HindIII	AAGCTT	1 cut(s)	349
HpaI	GTTAAC	1 cut(s)	621
HpaII	CCGG	2 cut(s)	525, 2174
HphI	GGTGA	3 cut(s)	695, 1002, 1031
Hpy166II	GTNNAC	4 cut(s)	621, 1205, 1948, 2563
Hpy8I	GTNNAC	4 cut(s)	621, 1205, 1948, 2563
Hpy99I	CGWCG	1 cut(s)	120
HpyCH4III	ACNGT	5 cut(s)	1349, 1814, 1900, 2389, 2617
HpyCH4IV	ACGT	5 cut(s)	157, 644, 1294, 1920, 2100
HpyF10VI	GCNNNNNNNGC	4 cut(s)	265, 500, 894, 923
HpySE526I	ACGT	5 cut(s)	157, 644, 1294, 1920, 2100
Hsp92I	GRCGYC	2 cut(s)	2171, 2329
HspAI	GCGC	5 cut(s)	143, 312, 1098, 2083, 2171
KasI	GGCGCC	1 cut(s)	2170
KroI	GCCGGC	1 cut(s)	2173
KroNI	GCCGGC	1 cut(s)	2175
Ksp22I	TGATCA	1 cut(s)	1537
KspAI	GTTAAC	1 cut(s)	621
Kzo9I	GATC	8 cut(s)	112, 150, 466, 1174, 1321, 1537, 1962, 2640
LguI	GCTCTTC	1 cut(s)	1560
LmnI	GCTCC	2 cut(s)	532, 2057
Lsp1109I	GCAGC	7 cut(s)	1012, 1015, 1218, 1378, 1813, 1816, 2512
LweI	GCATC	8 cut(s)	619, 619, 663, 838, 1214, 1249, 1540, 1849
MaeI	CTAG	5 cut(s)	1121, 1448, 1794, 1943, 2492
MaeII	ACGT	5 cut(s)	157, 644, 1294, 1920, 2100
MaeIII	GTNAC	4 cut(s)	951, 1814, 2089, 2389
MalI	GATC	8 cut(s)	114, 152, 468, 1176, 1323, 1539, 1964, 2642
MboI	GATC	8 cut(s)	112, 150, 466, 1174, 1321, 1537, 1962, 2640
MfeI	CAATTG	1 cut(s)	1958
MflI	RGATCY	2 cut(s)	150, 1321
MhlI	GDGCHC	3 cut(s)	2042, 2321, 2565
MlsI	TGGCCA	1 cut(s)	2399
MluNI	TGGCCA	1 cut(s)	2399
Mly113I	GGCGCC	1 cut(s)	2171
MlyI	GAGTC	5 cut(s)	343, 427, 728, 1281, 1515
MmeI	TCCRAC	4 cut(s)	178, 1130, 1903, 2097
Mox20I	TGGCCA	1 cut(s)	2399
Mph1103I	ATGCAT	4 cut(s)	6, 270, 656, 2068
MroNI	GCCGGC	1 cut(s)	2173
MroXI	GAANNNNTTC	2 cut(s)	452, 1443
MscI	TGGCCA	1 cut(s)	2399
MslI	CAYNNNNRTG	4 cut(s)	1222, 1305, 1434, 2412
Msp20I	TGGCCA	1 cut(s)	2399
MspI	CCGG	2 cut(s)	525, 2174
MspR9I	CCNGG	2 cut(s)	321, 526
MunI	CAATTG	1 cut(s)	1958
Mva1269I	GAATGC	5 cut(s)	177, 314, 554, 1987, 2066
MvaI	CCWGG	1 cut(s)	321
MwoI	GCNNNNNNNGC	4 cut(s)	265, 500, 894, 923
NaeI	GCCGGC	1 cut(s)	2175
NarI	GGCGCC	1 cut(s)	2171
NciI	CCSGG	1 cut(s)	526
NdeI	CATATG	1 cut(s)	484
NdeII	GATC	8 cut(s)	112, 150, 466, 1174, 1321, 1537, 1962, 2640
NgoMIV	GCCGGC	1 cut(s)	2173
NmuCI	GTSAC	1 cut(s)	951
NsiI	ATGCAT	4 cut(s)	6, 270, 656, 2068
NspI	RCATGY	4 cut(s)	139, 654, 2207, 2551
NspV	TTCGAA	1 cut(s)	2289
PaeI	GCATGC	1 cut(s)	654
PaeR7I	CTCGAG	1 cut(s)	1739
PagI	TCATGA	2 cut(s)	580, 972
PasI	CCCWGGG	1 cut(s)	320
PceI	AGGCCT	1 cut(s)	92
PciI	ACATGT	2 cut(s)	2203, 2547
PciSI	GCTCTTC	1 cut(s)	1560
PcsI	WCGNNNNNNNCGW	1 cut(s)	1291
PctI	GAATGC	5 cut(s)	177, 314, 554, 1987, 2066
PdiI	GCCGGC	1 cut(s)	2175
PdmI	GAANNNNTTC	2 cut(s)	452, 1443
PfeI	GAWTC	8 cut(s)	343, 797, 820, 942, 1439, 1994, 2363, 2589
PfoI	TCCNGGA	1 cut(s)	524
PkrI	GCNGC	7 cut(s)	1027, 1030, 1233, 1393, 1828, 1831, 2502
PleI	GAGTC	5 cut(s)	342, 426, 727, 1281, 1515
PluTI	GGCGCC	1 cut(s)	2174
PpsI	GAGTC	5 cut(s)	342, 426, 727, 1281, 1515
Ppu21I	YACGTR	1 cut(s)	1295
PpuMI	RGGWCCY	3 cut(s)	596, 996, 2533
PscI	ACATGT	2 cut(s)	2203, 2547
PshAI	GACNNNNGTC	1 cut(s)	702
PshBI	ATTAAT	1 cut(s)	2046
Psp5II	RGGWCCY	3 cut(s)	596, 996, 2533
Psp6I	CCWGG	1 cut(s)	319
PspFI	CCCAGC	1 cut(s)	377
PspGI	CCWGG	1 cut(s)	319
PspPI	GGNCC	9 cut(s)	48, 289, 508, 596, 996, 1875, 2222, 2255, 2533
PspPPI	RGGWCCY	3 cut(s)	596, 996, 2533
PspXI	VCTCGAGB	1 cut(s)	1739
PstI	CTGCAG	2 cut(s)	1236, 1321
PstNI	CAGNNNCTG	4 cut(s)	421, 740, 1227, 1262
PsuI	RGATCY	2 cut(s)	150, 1321
RsaI	GTAC	5 cut(s)	980, 1206, 1630, 1811, 2280
RsaNI	GTAC	5 cut(s)	979, 1205, 1629, 1810, 2279
RseI	CAYNNNNRTG	4 cut(s)	1222, 1305, 1434, 2412
SapI	GCTCTTC	1 cut(s)	1560
SatI	GCNGC	7 cut(s)	1026, 1029, 1232, 1392, 1827, 1830, 2501
Sau3AI	GATC	8 cut(s)	112, 150, 466, 1174, 1321, 1537, 1962, 2640
Sau96I	GGNCC	9 cut(s)	48, 289, 508, 596, 996, 1875, 2222, 2255, 2533
SchI	GAGTC	5 cut(s)	343, 427, 728, 1281, 1515
ScrFI	CCNGG	2 cut(s)	321, 526
SduI	GDGCHC	3 cut(s)	2042, 2321, 2565
SfaNI	GCATC	8 cut(s)	619, 619, 663, 838, 1214, 1249, 1540, 1849
SfcI	CTRYAG	3 cut(s)	106, 1232, 1317
SfoI	GGCGCC	1 cut(s)	2172
Sfr274I	CTCGAG	1 cut(s)	1739
SfuI	TTCGAA	1 cut(s)	2289
SinI	GGWCC	5 cut(s)	508, 596, 996, 2222, 2533
SlaI	CTCGAG	1 cut(s)	1739
SmiMI	CAYNNNNRTG	4 cut(s)	1222, 1305, 1434, 2412
SmlI	CTYRAG	1 cut(s)	1739
SmoI	CTYRAG	1 cut(s)	1739
SnaBI	TACGTA	1 cut(s)	1295
SpeI	ACTAGT	2 cut(s)	1942, 2491
SphI	GCATGC	1 cut(s)	654
SseBI	AGGCCT	1 cut(s)	92
SsiI	CCGC	3 cut(s)	175, 1864, 2369
SspDI	GGCGCC	1 cut(s)	2170
SspI	AATATT	2 cut(s)	780, 1613
SspMI	CTAG	5 cut(s)	1121, 1448, 1794, 1943, 2492
StuI	AGGCCT	1 cut(s)	92
StyD4I	CCNGG	2 cut(s)	319, 524
StyI	CCWWGG	3 cut(s)	30, 1363, 2217
TaaI	ACNGT	5 cut(s)	1349, 1814, 1900, 2389, 2617
TaiI	ACGT	5 cut(s)	160, 647, 1297, 1923, 2103
TaqI	TCGA	8 cut(s)	717, 1177, 1209, 1285, 1721, 1740, 1965, 2289
TaqII	GACCGA	1 cut(s)	2239
TatI	WGTACW	2 cut(s)	1204, 1628
TfiI	GAWTC	8 cut(s)	343, 797, 820, 942, 1439, 1994, 2363, 2589
TseFI	GTSAC	1 cut(s)	951
TseI	GCWGC	7 cut(s)	1025, 1028, 1231, 1391, 1826, 1829, 2500
Tsp45I	GTSAC	1 cut(s)	951
TspGWI	ACGGA	3 cut(s)	225, 1899, 2296
VneI	GTGCAC	1 cut(s)	2561
VpaK11BI	GGWCC	5 cut(s)	508, 596, 996, 2222, 2533
VspI	ATTAAT	1 cut(s)	2046
XagI	CCTNNNNNAGG	2 cut(s)	831, 2356
XapI	RAATTY	6 cut(s)	199, 520, 724, 808, 1787, 2291
XbaI	TCTAGA	2 cut(s)	1120, 1447
XceI	RCATGY	4 cut(s)	139, 654, 2207, 2551
XcmI	CCANNNNNNNNNTGG	2 cut(s)	79, 989
XhoI	CTCGAG	1 cut(s)	1739
XmnI	GAANNNNTTC	2 cut(s)	452, 1443
XspI	CTAG	5 cut(s)	1121, 1448, 1794, 1943, 2492
Zsp2I	ATGCAT	4 cut(s)	6, 270, 656, 2068

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.