Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr5

Orthogroup ID

OG0001708

Physical Location & Seq

Genomic Coordinates Forward (+)

18633915 .. 18643612

Gene Structure 9698 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw5G015210.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1992 bp

ATGGAAAATATCACGCAGAGGTTAGCCACATTTCCAAGGACAAATGGCACAGCCGAAGTAAATACTGATGTTCTGGTAGTAAATTGGTTGTTAGATAATCAACTTCATGATGGCTCATGGGGTCCTCCTAATCTGCATCAGTTGTTAACAAAAGATGCCCTCTTATCCACGTTAGCATGCATCCTTGCACTAAAGCGATGGAATATTGGTGAAGAACTAATTGACAGGGGTCTGCATTTTATCCAGTCAAATTTAGCTTCAGCTACTGATGGAGAGCAACCATCTCCTGTTGGGTTTGATATAATATTTCCTTCCATGATTGAATCTGCAATGAATTTGGATGTGACATACAAACTGAGCTTAACATGCAGGTTTGGCCTTGTTATTGGAATCATAGAATGTTTTCTTTCGTTACTGGAAGGAATCGGAGAGTCACAGGACTGGGAGATGGTCATGAAGTACCAGAAGAAGAATGGGTCCTTGTTTAACTCACCATCAACTACTGCTGCTGCTTTTACTCACCTCAACAATGCTGGTTGTCTTAGTTATCTACGCTCACTCATAGAAAAGTTTGGAAGGGCAGTTCCAACGATTTATCCTCTAGGTAACTATGCTCACCTTTCCATTGTTTCCAGTCTTGAAAGTATGGGCGTTGATCGACATTTCAGGAAGGAAATAAGGAGTGTACTCGATGAAACATACAGCATGACATTACCCAACAAACAATGCTGGCTGCAGGGGGATGAAGATATATTTTCAGATGCTGGCACCTGTGCTATGGCATTTCGACTCTTACGTCTTCATGGATATGATGTTTCTGCATATGATCCATTAAGTCAATTTTCAGAAGACAGTTTCTTCAATTCCCTTGGAGGATATCTGAAGGACACTGGTGTTGCCTTAGAACTTTTTAGGGCTTCAGAAGTCATCATTCATCCAGATGAATCAGAATTAGCAAATAACTTAACTCAGGCTCATAAACTCAATAAGCATATTGATCAGGTGGATGATGCTCTTCGGTTTCCTTCCTATGCAACTCTGGGTAGGTTATCAAGCAGGAAAGCAATAAAATATTACAACACTTACAGTACAAAGATTTTAAAATCTTCTTACTGTTGTTCAAATGTTGGGAATGAAGACTTCCTAAAACTGGCAGTGGAAGGCTTCAATATGTGTCAATCGATACATCGGGAAGAACTCGAGTATCTTTCAAGGTGGGTTGTCGAGAGCAGATTAGACAAGCTAGAATTTGCTAGGCAGAAGCAGGCATACAATACTTCTCTGCTGCTGCAACCCTTTTCCCTCCTGAACTATCAGATGCCCGCATATCATGGGCCAAAAACGGAGTACTTACAACTGTGGGATGTGAATGCAAAAACTGATTCTTGTTCTGAGCAAGTTGAAATCATATTTTCAGCAATTAAGAGCACAATTAATGAGATTGGAGCCAATGCATTCCCACGGCAACAGTGCAGTGTAACAAATCACGTCATAGAGATTTGGTTGGATTTGGTTAAGTCTATGCTGAAGGAAGCTCAGTGGTTGTTAAACAATTCGGTGCCGGCACTGGATGAATACATGGAAAATGCATATGTAGCATTTGCCTTGGGACCGATTGTGCTTCCAGCTCTCTATTTGGTAGGGCCTAAGCTCTCAGAGGAGGCTGTATGGAGTTTCGAATTCCACCAGTTGTATAAACTTATGAGCACCTGTGGGCGTCTTCTCAATGATATGCAAACCTTTAAGAGGGAATCTGCAGAAGGGAAGCTAAATGCTGTTACATTGGCCATGCTTCATGGCAATGGCAATGCTACTGAAGAAGAGACCTTCAGTGTGAAGAGCATTATAGCCATTAAGAGGAGAGAACTGCAAAGACTAGTTTTGCAGAAGGATAGCTTAGTTCCAAGAGCTTGCAAGGACCTGTTTTGGAACATGTGCAAAATTGTGCACCTATTTTATGCAAAGCACGATGGATTCACTGGCATGACTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006629 GO:0006720 GO:0006721 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009685 GO:0009686 GO:0009719 GO:0009725 GO:0009739 GO:0009740 GO:0009755 GO:0009899 GO:0009987 GO:0010033 GO:0010333 GO:0010476 GO:0010817 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016829 GO:0016835 GO:0016838 GO:0019752 GO:0023052 GO:0032870 GO:0033993 GO:0042221 GO:0042445 GO:0042446 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0065008 GO:0070887 GO:0071229 GO:0071310 GO:0071370 GO:0071396 GO:0071495 GO:0071704 GO:1901576 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko00904 ko01100 ko01110 map00904 map01100 map01110

Pfam Domains

Protein Families

Terpene_synth Terpene_synth_C

Protein Analysis

663

Amino Acids

74.9

Weight (kDa)

5.65

Isoelectric Point (pI)

47.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Terpene_synth	PF01397	147 - 326	1.1e-29	Terpene synthase, N-terminal domain
Terpene_synth_C	PF03936	448 - 614	6.5e-40	Terpene synthase family, metal binding domain
Terpene_syn_C_2	PF19086	448 - 608	5.1e-30	Terpene synthase family 2, C-terminal metal binding

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001708)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G79460
fragaria_vesca	FvH4_3g14116 FvH4_3g14130 FvH4_3g14131 FvH4_3g16270
malus_domestica	MD03G1187200.v1.1 MD10G1215700.v1.1 MD10G1215900.v1.1 MD10G1216200.v1.1
prunus_persica	Prupe.4G128500_v2.0.a1 Prupe.4G128500_v2.0.a1 Prupe.4G128500_v2.0.a1 Prupe.4G128600_v2.0.a1 Prupe.4G128700_v2.0.a1
pyrus_communis	pycom03g13990 pycom10g18220
rosa_chinensis	RchiOBHm_Chr5g0023441 RchiOBHm_Chr5g0023471 RchiOBHm_Chr5g0023641
rosa_laevigata	RLG00000032721
rosa_multiflora	Rmu_co8406553.1_g000001 Rmu_sc0004593.1_g000004 Rmu_sc0005664.1_g000001 Rmu_sc0005664.1_g000005
rosa_roxburghii	Rroxscaffold_1G00055600 Rroxscaffold_1G00055620 Rroxscaffold_1G00055910
rosa_rugosa	Rorug05G0079200 Rorug05G0079300
rosa_samantha	Rh5AG166100 Rh5AG166200 Rh5AG166300 Rh5AG167600 Rh5AG168000 Rh5BG165300 Rh5BG165500 Rh5CG180300 Rh5CG180400 Rh5CG181400 Rh5DG166800 Rh5DG166900 Rh5DG167800
rosa_wichuraiana	Rw0G019600 Rw5G014950 Rw5G014970 Rw5G015110 Rw5G015120 Rw5G015210

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	795
Acc36I	ACCTGC	1 cut(s)	360
AccB1I	GGYRCC	2 cut(s)	767, 1558
AciI	CCGC	1 cut(s)	1323
AclWI	GGATC	1 cut(s)	821
AcoI	YGGCCR	1 cut(s)	1785
AcsI	RAATTY	4 cut(s)	250, 334, 1247, 1679
AcuI	CTGAAG	6 cut(s)	243, 902, 903, 1547, 1813, 1836
AcyI	GRCGYC	1 cut(s)	1717
AfaI	GTAC	4 cut(s)	461, 687, 1090, 1349
AfiI	CCNNNNNNNGG	3 cut(s)	1150, 1746, 1857
AflIII	ACRYGT	1 cut(s)	1932
AgsI	TTSAA	7 cut(s)	323, 641, 862, 1122, 1168, 1212, 1403
AhlI	ACTAGT	1 cut(s)	1876
AjiI	CACGTC	1 cut(s)	1489
AloI	GAACNNNNNNTCC	2 cut(s)	567, 599
Alw21I	GWGCWC	3 cut(s)	1430, 1709, 1950
Alw26I	GTCTC	1 cut(s)	1817
Alw44I	GTGCAC	1 cut(s)	1946
AlwI	GGATC	1 cut(s)	821
AlwNI	CAGNNNCTG	2 cut(s)	266, 764
Ama87I	CYCGRG	1 cut(s)	1199
AoxI	GGCC	4 cut(s)	376, 1334, 1643, 1785
ApaLI	GTGCAC	1 cut(s)	1946
ApeKI	GCWGC	5 cut(s)	506, 509, 733, 1285, 1288
ApoI	RAATTY	4 cut(s)	250, 334, 1247, 1679
AseI	ATTAAT	1 cut(s)	1434
Asp700I	GAANNNNTTC	1 cut(s)	402
AspS9I	GGNCC	6 cut(s)	122, 477, 1334, 1610, 1643, 1918
AsuHPI	GGTGA	4 cut(s)	221, 483, 512, 608
AsuII	TTCGAA	1 cut(s)	1677
AvaI	CYCGRG	1 cut(s)	1199
AvaII	GGWCC	4 cut(s)	122, 477, 1610, 1918
BaeGI	GKGCMC	1 cut(s)	1950
BaeI	ACNNNNGTAYC	2 cut(s)	1187, 1220
BalI	TGGCCA	1 cut(s)	1787
BanI	GGYRCC	2 cut(s)	767, 1558
BarI	GAAGNNNNNNTAC	2 cut(s)	1253, 1285
BbsI	GAAGAC	4 cut(s)	791, 855, 1143, 1712
Bbv12I	GWGCWC	3 cut(s)	1430, 1709, 1950
BbvI	GCAGC	5 cut(s)	493, 496, 720, 1272, 1275
BccI	CCATC	7 cut(s)	104, 192, 263, 289, 442, 502, 1964
BceAI	ACGGC	1 cut(s)	1478
BclI	TGATCA	1 cut(s)	997
BcoDI	GTCTC	1 cut(s)	1817
BcuI	ACTAGT	1 cut(s)	1876
BfaI	CTAG	4 cut(s)	602, 1244, 1254, 1877
BfmI	CTRYAG	2 cut(s)	734, 1755
BfuAI	ACCTGC	1 cut(s)	360
BisI	GCNGC	5 cut(s)	507, 510, 734, 1286, 1289
BlsI	GCNGC	5 cut(s)	508, 511, 735, 1287, 1290
BmcAI	AGTACT	1 cut(s)	1349
Bme18I	GGWCC	4 cut(s)	122, 477, 1610, 1918
BmeT110I	CYCGRG	1 cut(s)	1199
BmgBI	CACGTC	1 cut(s)	1489
BmgT120I	GGNCC	6 cut(s)	122, 477, 1334, 1610, 1643, 1918
BmiI	GGNNCC	6 cut(s)	123, 478, 769, 1447, 1560, 1611
BmrI	ACTGGG	1 cut(s)	451
BmsI	GCATC	6 cut(s)	145, 145, 189, 751, 1000, 1308
BmuI	ACTGGG	1 cut(s)	451
BoxI	GACNNNNGTC	1 cut(s)	228
BpiI	GAAGAC	4 cut(s)	791, 855, 1143, 1712
Bpu10I	CCTNAGC	1 cut(s)	1647
Bpu14I	TTCGAA	1 cut(s)	1677
Bsa29I	ATCGAT	1 cut(s)	1181
BsaHI	GRCGYC	1 cut(s)	1717
BsaI	GGTCTC	1 cut(s)	1817
BsaJI	CCNNGG	4 cut(s)	35, 868, 1460, 1605
Bsc4I	CCNNNNNNNGG	3 cut(s)	1150, 1746, 1857
Bse118I	RCCGGY	1 cut(s)	1561
Bse1I	ACTGG	9 cut(s)	244, 420, 446, 633, 895, 1155, 1572, 1687, 1984
Bse3DI	GCAATG	3 cut(s)	336, 1807, 1813
BseCI	ATCGAT	1 cut(s)	1181
BseDI	CCNNGG	4 cut(s)	35, 868, 1460, 1605
BseGI	GGATG	7 cut(s)	180, 346, 748, 934, 1012, 1369, 1576
BseLI	CCNNNNNNNGG	3 cut(s)	1150, 1746, 1857
BseMI	GCAATG	3 cut(s)	336, 1807, 1813
BseMII	CTCAG	5 cut(s)	347, 983, 1383, 1550, 1668
BseNI	ACTGG	9 cut(s)	244, 420, 446, 633, 895, 1155, 1572, 1687, 1984
BseRI	GAGGAG	2 cut(s)	1673, 1873
BseSI	GKGCMC	1 cut(s)	1950
BseXI	GCAGC	5 cut(s)	493, 496, 720, 1272, 1275
BsgI	GTGCAG	1 cut(s)	1492
BshFI	GGCC	4 cut(s)	378, 1336, 1645, 1787
BshNI	GGYRCC	2 cut(s)	767, 1558
BshVI	ATCGAT	1 cut(s)	1181
BsiHKAI	GWGCWC	3 cut(s)	1430, 1709, 1950
BsiHKCI	CYCGRG	1 cut(s)	1199
BsiSI	CCGG	1 cut(s)	1562
BslFI	GGGAC	1 cut(s)	1623
BslI	CCNNNNNNNGG	3 cut(s)	1150, 1746, 1857
BsmAI	GTCTC	1 cut(s)	1817
BsmFI	GGGAC	1 cut(s)	1623
BsmI	GAATGC	2 cut(s)	1375, 1454
BsnI	GGCC	4 cut(s)	378, 1336, 1645, 1787
Bso31I	GGTCTC	1 cut(s)	1817
BsoBI	CYCGRG	1 cut(s)	1199
Bsp119I	TTCGAA	1 cut(s)	1677
Bsp1286I	GDGCHC	3 cut(s)	1430, 1709, 1950
Bsp143I	GATC	3 cut(s)	655, 826, 997
BspACI	CCGC	1 cut(s)	1323
BspANI	GGCC	4 cut(s)	378, 1336, 1645, 1787
BspCNI	CTCAG	5 cut(s)	348, 982, 1384, 1549, 1667
BspDI	ATCGAT	1 cut(s)	1181
BspHI	TCATGA	2 cut(s)	106, 453
BspLI	GGNNCC	6 cut(s)	123, 478, 769, 1447, 1560, 1611
BspMAI	CTGCAG	2 cut(s)	738, 1759
BspMI	ACCTGC	1 cut(s)	360
BspPI	GGATC	1 cut(s)	821
BspQI	GCTCTTC	2 cut(s)	1020, 1832
BspT104I	TTCGAA	1 cut(s)	1677
BspT107I	GGYRCC	2 cut(s)	767, 1558
BspTNI	GGTCTC	1 cut(s)	1817
BsrDI	GCAATG	3 cut(s)	336, 1807, 1813
BsrFI	RCCGGY	1 cut(s)	1561
BsrI	ACTGG	9 cut(s)	244, 420, 446, 633, 895, 1155, 1572, 1687, 1984
BssAI	RCCGGY	1 cut(s)	1561
BssECI	CCNNGG	4 cut(s)	35, 868, 1460, 1605
BssMI	GATC	3 cut(s)	655, 826, 997
BssNI	GRCGYC	1 cut(s)	1717
BssT1I	CCWWGG	3 cut(s)	35, 868, 1605
Bst4CI	ACNGT	5 cut(s)	854, 1088, 1115, 1359, 1470
Bst6I	CTCTTC	3 cut(s)	1020, 1815, 1832
BstACI	GRCGYC	1 cut(s)	1717
BstBI	TTCGAA	1 cut(s)	1677
BstC8I	GCNNGC	7 cut(s)	178, 731, 766, 1266, 1323, 1563, 1912
BstDEI	CTNAG	9 cut(s)	356, 542, 901, 969, 1392, 1536, 1647, 1654, 1897
BstDSI	CCRYGG	1 cut(s)	1460
BstENI	CCTNNNNNAGG	1 cut(s)	1744
BstF5I	GGATG	7 cut(s)	180, 346, 748, 934, 1012, 1369, 1576
BstKTI	GATC	3 cut(s)	658, 829, 1000
BstMAI	GTCTC	1 cut(s)	1817
BstMBI	GATC	3 cut(s)	655, 826, 997
BstMWI	GCNNNNNNNGC	3 cut(s)	366, 375, 1595
BstNSI	RCATGY	3 cut(s)	180, 369, 1936
BstPAI	GACNNNNGTC	1 cut(s)	228
BstSFI	CTRYAG	2 cut(s)	734, 1755
BstSLI	GKGCMC	1 cut(s)	1950
BstV1I	GCAGC	5 cut(s)	493, 496, 720, 1272, 1275
BstV2I	GAAGAC	4 cut(s)	791, 855, 1143, 1712
Bsu15I	ATCGAT	1 cut(s)	1181
BsuRI	GGCC	4 cut(s)	378, 1336, 1645, 1787
BsuTUI	ATCGAT	1 cut(s)	1181
BtgI	CCRYGG	1 cut(s)	1460
BtgZI	GCGATG	1 cut(s)	211
BtrI	CACGTC	1 cut(s)	1489
BtsCI	GGATG	7 cut(s)	180, 346, 748, 934, 1012, 1369, 1576
BtsI	GCAGTG	2 cut(s)	1161, 1480
BtsIMutI	CAGTG	8 cut(s)	888, 1161, 1475, 1480, 1544, 1565, 1837, 1977
BveI	ACCTGC	1 cut(s)	360
Cac8I	GCNNGC	7 cut(s)	178, 731, 766, 1266, 1323, 1563, 1912
CaiI	CAGNNNCTG	2 cut(s)	266, 764
CciI	TCATGA	2 cut(s)	106, 453
Cfr10I	RCCGGY	1 cut(s)	1561
Cfr13I	GGNCC	6 cut(s)	122, 477, 1334, 1610, 1643, 1918
ClaI	ATCGAT	1 cut(s)	1181
CseI	GACGC	1 cut(s)	1706
Csp6I	GTAC	4 cut(s)	460, 686, 1089, 1348
CviQI	GTAC	4 cut(s)	460, 686, 1089, 1348
DdeI	CTNAG	9 cut(s)	356, 542, 901, 969, 1392, 1536, 1647, 1654, 1897
DpnI	GATC	3 cut(s)	657, 828, 999
DpnII	GATC	3 cut(s)	655, 826, 997
DraI	TTTAAA	1 cut(s)	1101
DrdI	GACNNNNNNGTC	1 cut(s)	795
DseDI	GACNNNNNNGTC	1 cut(s)	795
EaeI	YGGCCR	1 cut(s)	1785
Eam1104I	CTCTTC	3 cut(s)	1020, 1815, 1832
EarI	CTCTTC	3 cut(s)	1020, 1815, 1832
Eco130I	CCWWGG	3 cut(s)	35, 868, 1605
Eco31I	GGTCTC	1 cut(s)	1817
Eco32I	GATATC	1 cut(s)	878
Eco47I	GGWCC	4 cut(s)	122, 477, 1610, 1918
Eco57I	CTGAAG	6 cut(s)	243, 902, 903, 1547, 1813, 1836
Eco88I	CYCGRG	1 cut(s)	1199
EcoNI	CCTNNNNNAGG	1 cut(s)	1744
EcoO109I	RGGNCCY	4 cut(s)	122, 477, 1643, 1918
EcoRI	GAATTC	1 cut(s)	1679
EcoRV	GATATC	1 cut(s)	878
EcoT14I	CCWWGG	3 cut(s)	35, 868, 1605
EcoT22I	ATGCAT	3 cut(s)	182, 1456, 1591
ErhI	CCWWGG	3 cut(s)	35, 868, 1605
FalI	AAGNNNNNCTT	2 cut(s)	1880, 1912
FaqI	GGGAC	1 cut(s)	1623
FauI	CCCGC	1 cut(s)	1330
FauNDI	CATATG	2 cut(s)	823, 1591
FbaI	TGATCA	1 cut(s)	997
Fnu4HI	GCNGC	5 cut(s)	507, 510, 734, 1286, 1289
FokI	GGATG	7 cut(s)	167, 353, 755, 921, 1019, 1376, 1583
Fsp4HI	GCNGC	5 cut(s)	507, 510, 734, 1286, 1289
FspBI	CTAG	4 cut(s)	602, 1244, 1254, 1877
GluI	GCNGC	5 cut(s)	507, 510, 734, 1286, 1289
HaeIII	GGCC	4 cut(s)	378, 1336, 1645, 1787
HapII	CCGG	1 cut(s)	1562
HgaI	GACGC	1 cut(s)	1706
Hin1I	GRCGYC	1 cut(s)	1717
HincII	GTYRAC	1 cut(s)	147
HindII	GTYRAC	1 cut(s)	147
HinfI	GANTC	9 cut(s)	323, 390, 423, 431, 789, 944, 1382, 1751, 1974
HpaI	GTTAAC	1 cut(s)	147
HpaII	CCGG	1 cut(s)	1562
HphI	GGTGA	4 cut(s)	221, 483, 512, 608
Hpy166II	GTNNAC	3 cut(s)	147, 686, 1948
Hpy188I	TCNGA	9 cut(s)	428, 760, 847, 882, 922, 949, 1317, 1393, 1657
Hpy188III	TCNNGA	8 cut(s)	107, 454, 638, 667, 938, 1190, 1225, 1306
Hpy8I	GTNNAC	3 cut(s)	147, 686, 1948
HpyCH4III	ACNGT	5 cut(s)	854, 1088, 1115, 1359, 1470
HpyCH4IV	ACGT	3 cut(s)	170, 796, 1488
HpyF10VI	GCNNNNNNNGC	3 cut(s)	366, 375, 1595
HpyF3I	CTNAG	9 cut(s)	356, 542, 901, 969, 1392, 1536, 1647, 1654, 1897
HpySE526I	ACGT	3 cut(s)	170, 796, 1488
Hsp92I	GRCGYC	1 cut(s)	1717
KroI	GCCGGC	1 cut(s)	1561
KroNI	GCCGGC	1 cut(s)	1563
Ksp22I	TGATCA	1 cut(s)	997
KspAI	GTTAAC	1 cut(s)	147
Kzo9I	GATC	3 cut(s)	655, 826, 997
LguI	GCTCTTC	2 cut(s)	1020, 1832
LmnI	GCTCC	1 cut(s)	1445
Lsp1109I	GCAGC	5 cut(s)	493, 496, 720, 1272, 1275
LweI	GCATC	6 cut(s)	145, 145, 189, 751, 1000, 1308
MaeI	CTAG	4 cut(s)	602, 1244, 1254, 1877
MaeII	ACGT	3 cut(s)	170, 796, 1488
MaeIII	GTNAC	6 cut(s)	343, 411, 432, 605, 1477, 1777
MalI	GATC	3 cut(s)	657, 828, 999
MboI	GATC	3 cut(s)	655, 826, 997
MhlI	GDGCHC	3 cut(s)	1430, 1709, 1950
MlsI	TGGCCA	1 cut(s)	1787
MluNI	TGGCCA	1 cut(s)	1787
MlyI	GAGTC	2 cut(s)	440, 783
MmeI	TCCRAC	2 cut(s)	611, 1485
Mox20I	TGGCCA	1 cut(s)	1787
Mph1103I	ATGCAT	3 cut(s)	182, 1456, 1591
MroNI	GCCGGC	1 cut(s)	1561
MroXI	GAANNNNTTC	1 cut(s)	402
MscI	TGGCCA	1 cut(s)	1787
MslI	CAYNNNNRTG	4 cut(s)	807, 939, 1800, 1982
Msp20I	TGGCCA	1 cut(s)	1787
MspI	CCGG	1 cut(s)	1562
Mva1269I	GAATGC	2 cut(s)	1375, 1454
MwoI	GCNNNNNNNGC	3 cut(s)	366, 375, 1595
NaeI	GCCGGC	1 cut(s)	1563
NdeI	CATATG	2 cut(s)	823, 1591
NdeII	GATC	3 cut(s)	655, 826, 997
NgoMIV	GCCGGC	1 cut(s)	1561
NlaIV	GGNNCC	6 cut(s)	123, 478, 769, 1447, 1560, 1611
NmuCI	GTSAC	2 cut(s)	343, 432
NsiI	ATGCAT	3 cut(s)	182, 1456, 1591
NspI	RCATGY	3 cut(s)	180, 369, 1936
NspV	TTCGAA	1 cut(s)	1677
PaeI	GCATGC	1 cut(s)	180
PaeR7I	CTCGAG	1 cut(s)	1199
PagI	TCATGA	2 cut(s)	106, 453
PciI	ACATGT	1 cut(s)	1932
PciSI	GCTCTTC	2 cut(s)	1020, 1832
PcsI	WCGNNNNNNNCGW	1 cut(s)	793
PctI	GAATGC	2 cut(s)	1375, 1454
PdiI	GCCGGC	1 cut(s)	1563
PdmI	GAANNNNTTC	1 cut(s)	402
PfeI	GAWTC	7 cut(s)	323, 390, 423, 944, 1382, 1751, 1974
PkrI	GCNGC	5 cut(s)	508, 511, 735, 1287, 1290
PleI	GAGTC	2 cut(s)	439, 783
PpsI	GAGTC	2 cut(s)	439, 783
PpuMI	RGGWCCY	3 cut(s)	122, 477, 1918
PscI	ACATGT	1 cut(s)	1932
PshAI	GACNNNNGTC	1 cut(s)	228
PshBI	ATTAAT	1 cut(s)	1434
Psp5II	RGGWCCY	3 cut(s)	122, 477, 1918
PspN4I	GGNNCC	6 cut(s)	123, 478, 769, 1447, 1560, 1611
PspPI	GGNCC	6 cut(s)	122, 477, 1334, 1610, 1643, 1918
PspPPI	RGGWCCY	3 cut(s)	122, 477, 1918
PspXI	VCTCGAGB	1 cut(s)	1199
PstI	CTGCAG	2 cut(s)	738, 1759
PstNI	CAGNNNCTG	2 cut(s)	266, 764
RsaI	GTAC	4 cut(s)	461, 687, 1090, 1349
RsaNI	GTAC	4 cut(s)	460, 686, 1089, 1348
RseI	CAYNNNNRTG	4 cut(s)	807, 939, 1800, 1982
SapI	GCTCTTC	2 cut(s)	1020, 1832
SatI	GCNGC	5 cut(s)	507, 510, 734, 1286, 1289
Sau3AI	GATC	3 cut(s)	655, 826, 997
Sau96I	GGNCC	6 cut(s)	122, 477, 1334, 1610, 1643, 1918
ScaI	AGTACT	1 cut(s)	1349
SchI	GAGTC	2 cut(s)	440, 783
SduI	GDGCHC	3 cut(s)	1430, 1709, 1950
SfaNI	GCATC	6 cut(s)	145, 145, 189, 751, 1000, 1308
SfcI	CTRYAG	2 cut(s)	734, 1755
Sfr274I	CTCGAG	1 cut(s)	1199
SfuI	TTCGAA	1 cut(s)	1677
SinI	GGWCC	4 cut(s)	122, 477, 1610, 1918
SlaI	CTCGAG	1 cut(s)	1199
SmiMI	CAYNNNNRTG	4 cut(s)	807, 939, 1800, 1982
SmlI	CTYRAG	1 cut(s)	1199
SmoI	CTYRAG	1 cut(s)	1199
SpeI	ACTAGT	1 cut(s)	1876
SphI	GCATGC	1 cut(s)	180
SsiI	CCGC	1 cut(s)	1323
SspI	AATATT	3 cut(s)	205, 306, 1073
SspMI	CTAG	4 cut(s)	602, 1244, 1254, 1877
StyI	CCWWGG	3 cut(s)	35, 868, 1605
TaaI	ACNGT	5 cut(s)	854, 1088, 1115, 1359, 1470
TaiI	ACGT	3 cut(s)	173, 799, 1491
TaqI	TCGA	7 cut(s)	658, 690, 787, 1181, 1200, 1224, 1677
TaqII	GACCGA	1 cut(s)	1627
TatI	WGTACW	3 cut(s)	685, 1088, 1347
TfiI	GAWTC	7 cut(s)	323, 390, 423, 944, 1382, 1751, 1974
TscAI	CASTG	8 cut(s)	895, 1161, 1475, 1480, 1544, 1572, 1837, 1984
TseFI	GTSAC	2 cut(s)	343, 432
TseI	GCWGC	5 cut(s)	506, 509, 733, 1285, 1288
Tsp45I	GTSAC	2 cut(s)	343, 432
TspGWI	ACGGA	1 cut(s)	1358
TspRI	CASTG	8 cut(s)	895, 1161, 1475, 1480, 1544, 1572, 1837, 1984
VneI	GTGCAC	1 cut(s)	1946
VpaK11BI	GGWCC	4 cut(s)	122, 477, 1610, 1918
VspI	ATTAAT	1 cut(s)	1434
XagI	CCTNNNNNAGG	1 cut(s)	1744
XapI	RAATTY	4 cut(s)	250, 334, 1247, 1679
XceI	RCATGY	3 cut(s)	180, 369, 1936
XcmI	CCANNNNNNNNNTGG	1 cut(s)	470
XhoI	CTCGAG	1 cut(s)	1199
XmnI	GAANNNNTTC	1 cut(s)	402
XspI	CTAG	4 cut(s)	602, 1244, 1254, 1877
ZrmI	AGTACT	1 cut(s)	1349
Zsp2I	ATGCAT	3 cut(s)	182, 1456, 1591

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.