Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0001288

Physical Location & Seq

Genomic Coordinates Reverse (-)

26653551 .. 26655300

Gene Structure 1750 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1175500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1002 bp

ATGGAGAGTGTGCTACCGAATAGGGAGAGAGCGATGGAGGAGTTGATCCAAGGGCGTGAACTGGCAAGTCAACTTAGTCGGATTTTTGAAAATAGGTCATTTCTTGTTGATGGTGCTGAGGGGGGATCGGCTGAAGGTATTGTTAATAAGATTTTGGGGTCATTTGTGAATACCCTTTTGATTATAAATGGGAAGGAGTCTGATCAAGAGTTTGTTTCTGATCAAATTCAAGGGAATGGTAGTGGTGGTAGTGGCAGTGGTGTTAGTGACGGTGGTAGTGGTGGTAGTGGTGATGGTGGCGGTGCAGATTCATCATCTTGGGATGCTAATCATGTTCATGCGGCGCCTGCTATTACGTCTGAAGATTATACTGAGGAGCAGATCAGTTGTAAGAGCACTTCAACCTTCAAGGATCGTAGAGGTTCTTACAAGAGAAGAAAGACTTTACAAAACTCTTGGACTAGAGACACTCCTACTTTGACTTACGATGGTCATGAATGGAGAAAGTACGGACAGAAATTTATTCGTAGTGCCAAGCATTCAAGAAACTACTTCAGATGCACTCACAAATTCGAGTGCAAAGCAACCAAACATGTGCAACAAATTCAAGATCATCCACCACTGTTTCGAACCACATATTTTGGGAATCACACCTGCAAAGACTACCTTAACGCTTCGGAATTGGTCTTGAATTCCGCAAGTCCTAGAGATTCTTCAAAGTTCATTTGTTTTGACAACGCCAACTGTTTGACAAACAAAGAAGAGCACCCTTTTTTCTCATCCTTTAAAGAAGATATGCCAACAACTGATCATATGGTGAAAAGTGACCACAACCATTCATCACCGTGTGATCATCTTGTGTCGAATGATCTGACGGCGTTTGAGTCTTCTGGTCTCATGAGCGGGTTCTCATCAAGCATCGATCAGTATGATTACGACGAGGAGTTTTCAAGGATTGTCGAGTCTTACTTCGAGAATGAAGTTTCGCAATACGGATTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0002218 GO:0002252 GO:0002253 GO:0002376 GO:0002682 GO:0002684 GO:0003674 GO:0003700 GO:0006355 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009627 GO:0009682 GO:0009719 GO:0009725 GO:0009751 GO:0009753 GO:0009755 GO:0009814 GO:0009862 GO:0009863 GO:0009864 GO:0009867 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010200 GO:0010243 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0014070 GO:0019219 GO:0019222 GO:0023052 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031347 GO:0031349 GO:0032870 GO:0035690 GO:0042221 GO:0042493 GO:0042742 GO:0043207 GO:0045087 GO:0045088 GO:0045089 GO:0045892 GO:0045934 GO:0046677 GO:0048518 GO:0048519 GO:0048523 GO:0048583 GO:0048584 GO:0048831 GO:0050776 GO:0050778 GO:0050789 GO:0050793 GO:0050794 GO:0050832 GO:0050896 GO:0051093 GO:0051171 GO:0051172 GO:0051239 GO:0051241 GO:0051252 GO:0051253 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0065007 GO:0070887 GO:0071229 GO:0071236 GO:0071310 GO:0071395 GO:0071407 GO:0071446 GO:0071495 GO:0080090 GO:0080134 GO:0098542 GO:0140110 GO:1900055 GO:1900056 GO:1901698 GO:1901700 GO:1901701 GO:1902679 GO:1903506 GO:1903507 GO:1905622 GO:2000024 GO:2000026 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WRKY

Protein Analysis

334

Amino Acids

37.19

Weight (kDa)

5.38

Isoelectric Point (pI)

49.8

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WRKY	PF03106	163 - 220	1.2e-22	WRKY DNA -binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001288)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G22570
fragaria_vesca	FvH4_6g09650 FvH4_6g09710 FvH4_6g09710 FvH4_6g09730 FvH4_6g09740 FvH4_6g09740 FvH4_6g09740 FvH4_6g09740 FvH4_6g09740 FvH4_6g09740 FvH4_6g09750 FvH4_6g09760
malus_domestica	MD04G1175500.v1.1 MD04G1175600.v1.1 MD12G1189200.v1.1 MD12G1189400.v1.1 MD12G1189600.v1.1 MD12G1189700.v1.1 MD12G1189900.v1.1
prunus_persica	Prupe.6G294900_v2.0.a1 Prupe.6G295000_v2.0.a1 Prupe.6G295100_v2.0.a1
pyrus_communis	pycom04g15480 pycom04g15490 pycom12g17650 pycom12g17690 pycom12g17700 pycom12g17730
rosa_chinensis	RchiOBHm_Chr3g0460351 RchiOBHm_Chr3g0460361
rosa_laevigata	RLG00000025018 RLG00000025019 RLG00000025027 RLG00000025113
rosa_multiflora	Rmu_sc0000449.1_g000005 Rmu_sc0004267.1_g000019
rosa_roxburghii	Rroxscaffold_6G00419560 Rroxscaffold_6G00419590
rosa_rugosa	Rorug03G0033500 Rorug03G0033500 Rorug03G0041400 Rorug03G0041800 Rorug03G0042000
rosa_samantha	Rh3AG100000 Rh3AG100100 Rh3BG094800 Rh3BG103900 Rh3BG104000 Rh3CG105700 Rh3CG105800 Rh3DG105300 Rh3DG105400
rosa_wichuraiana	Rw3G008480 Rw3G008490

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	185
AarI	CACCTGC	1 cut(s)	662
Acc36I	ACCTGC	1 cut(s)	662
AccB1I	GGYRCC	1 cut(s)	343
AccBSI	CCGCTC	1 cut(s)	903
AciI	CCGC	4 cut(s)	300, 341, 696, 903
AclWI	GGATC	3 cut(s)	40, 133, 420
AcsI	RAATTY	5 cut(s)	225, 518, 569, 603, 691
AcuI	CTGAAG	3 cut(s)	153, 381, 538
AcyI	GRCGYC	1 cut(s)	344
AdeI	CACNNNGTG	1 cut(s)	848
AfaI	GTAC	1 cut(s)	509
AflIII	ACRYGT	1 cut(s)	592
AgsI	TTSAA	9 cut(s)	89, 230, 402, 409, 543, 608, 691, 717, 951
Alw21I	GWGCWC	2 cut(s)	398, 768
Alw26I	GTCTC	2 cut(s)	459, 899
AlwI	GGATC	3 cut(s)	40, 133, 420
ApoI	RAATTY	5 cut(s)	225, 518, 569, 603, 691
AspLEI	GCGC	1 cut(s)	346
AsuHPI	GGTGA	3 cut(s)	302, 829, 834
AsuII	TTCGAA	1 cut(s)	628
BanI	GGYRCC	1 cut(s)	343
BarI	GAAGNNNNNNTAC	2 cut(s)	382, 414
BbsI	GAAGAC	1 cut(s)	879
Bbv12I	GWGCWC	2 cut(s)	398, 768
BbvCI	CCTCAGC	1 cut(s)	117
BccI	CCATC	4 cut(s)	28, 104, 287, 482
BceAI	ACGGC	1 cut(s)	891
BclI	TGATCA	4 cut(s)	202, 220, 808, 850
BcoDI	GTCTC	2 cut(s)	459, 899
BfaI	CTAG	2 cut(s)	462, 705
BfoI	RGCGCY	1 cut(s)	347
BfuAI	ACCTGC	1 cut(s)	662
BisI	GCNGC	1 cut(s)	342
BlsI	GCNGC	1 cut(s)	343
BmiI	GGNNCC	1 cut(s)	345
BmsI	GCATC	3 cut(s)	313, 548, 927
BpiI	GAAGAC	1 cut(s)	879
Bpu10I	CCTNAGC	1 cut(s)	117
Bpu14I	TTCGAA	1 cut(s)	628
Bsa29I	ATCGAT	1 cut(s)	921
BsaBI	GATNNNNATC	1 cut(s)	327
BsaHI	GRCGYC	1 cut(s)	344
BsaI	GGTCTC	1 cut(s)	899
BsaJI	CCNNGG	1 cut(s)	49
Bse1I	ACTGG	1 cut(s)	66
Bse8I	GATNNNNATC	1 cut(s)	327
BseCI	ATCGAT	1 cut(s)	921
BseDI	CCNNGG	1 cut(s)	49
BseGI	GGATG	3 cut(s)	328, 613, 779
BseJI	GATNNNNATC	1 cut(s)	327
BseMII	CTCAG	2 cut(s)	108, 363
BseNI	ACTGG	1 cut(s)	66
BseRI	GAGGAG	3 cut(s)	53, 389, 956
BsgI	GTGCAG	1 cut(s)	324
BshNI	GGYRCC	1 cut(s)	343
BshVI	ATCGAT	1 cut(s)	921
BsiHKAI	GWGCWC	2 cut(s)	398, 768
BsmAI	GTCTC	2 cut(s)	459, 899
BsmI	GAATGC	1 cut(s)	538
Bso31I	GGTCTC	1 cut(s)	899
Bsp119I	TTCGAA	1 cut(s)	628
Bsp1286I	GDGCHC	2 cut(s)	398, 768
BspACI	CCGC	4 cut(s)	300, 341, 696, 903
BspCNI	CTCAG	2 cut(s)	109, 364
BspDI	ATCGAT	1 cut(s)	921
BspHI	TCATGA	2 cut(s)	493, 897
BspLI	GGNNCC	1 cut(s)	345
BspMI	ACCTGC	1 cut(s)	662
BspPI	GGATC	3 cut(s)	40, 133, 420
BspQI	GCTCTTC	1 cut(s)	756
BspT104I	TTCGAA	1 cut(s)	628
BspT107I	GGYRCC	1 cut(s)	343
BspTNI	GGTCTC	1 cut(s)	899
BsrBI	CCGCTC	1 cut(s)	903
BsrI	ACTGG	1 cut(s)	66
BssECI	CCNNGG	1 cut(s)	49
BssNI	GRCGYC	1 cut(s)	344
BssT1I	CCWWGG	1 cut(s)	49
Bst4CI	ACNGT	4 cut(s)	272, 624, 746, 846
Bst6I	CTCTTC	1 cut(s)	756
BstACI	GRCGYC	1 cut(s)	344
BstBI	TTCGAA	1 cut(s)	628
BstC8I	GCNNGC	1 cut(s)	348
BstDEI	CTNAG	3 cut(s)	74, 117, 372
BstF5I	GGATG	3 cut(s)	328, 613, 779
BstH2I	RGCGCY	1 cut(s)	347
BstHHI	GCGC	1 cut(s)	346
BstMAI	GTCTC	2 cut(s)	459, 899
BstMWI	GCNNNNNNNGC	1 cut(s)	347
BstNSI	RCATGY	1 cut(s)	596
BstV2I	GAAGAC	1 cut(s)	879
Bsu15I	ATCGAT	1 cut(s)	921
BsuTUI	ATCGAT	1 cut(s)	921
BtgZI	GCGATG	1 cut(s)	47
BtsCI	GGATG	3 cut(s)	328, 613, 779
BtsI	GCAGTG	1 cut(s)	262
BtsIMutI	CAGTG	2 cut(s)	262, 620
BveI	ACCTGC	1 cut(s)	662
Cac8I	GCNNGC	1 cut(s)	348
CciI	TCATGA	2 cut(s)	493, 897
CfoI	GCGC	1 cut(s)	346
ClaI	ATCGAT	1 cut(s)	921
Csp6I	GTAC	1 cut(s)	508
CspCI	CAANNNNNGTGG	2 cut(s)	622, 657
CviAII	CATG	5 cut(s)	332, 338, 494, 593, 898
CviJI	RGCY	1 cut(s)	131
CviKI_1	RGCY	1 cut(s)	131
CviQI	GTAC	1 cut(s)	508
DdeI	CTNAG	3 cut(s)	74, 117, 372
DinI	GGCGCC	1 cut(s)	345
DraI	TTTAAA	1 cut(s)	787
DraIII	CACNNNGTG	1 cut(s)	848
Eam1104I	CTCTTC	1 cut(s)	756
EarI	CTCTTC	1 cut(s)	756
Eco130I	CCWWGG	1 cut(s)	49
Eco31I	GGTCTC	1 cut(s)	899
Eco57I	CTGAAG	3 cut(s)	153, 381, 538
EcoRI	GAATTC	1 cut(s)	691
EcoT14I	CCWWGG	1 cut(s)	49
EgeI	GGCGCC	1 cut(s)	345
EheI	GGCGCC	1 cut(s)	345
ErhI	CCWWGG	1 cut(s)	49
FaeI	CATG	5 cut(s)	335, 341, 497, 596, 901
FalI	AAGNNNNNCTT	4 cut(s)	427, 459, 651, 683
FatI	CATG	5 cut(s)	331, 337, 493, 592, 897
FauI	CCCGC	1 cut(s)	896
FauNDI	CATATG	1 cut(s)	813
FbaI	TGATCA	4 cut(s)	202, 220, 808, 850
Fnu4HI	GCNGC	1 cut(s)	342
FokI	GGATG	3 cut(s)	335, 600, 766
Fsp4HI	GCNGC	1 cut(s)	342
FspBI	CTAG	2 cut(s)	462, 705
GlaI	GCGC	1 cut(s)	345
GluI	GCNGC	1 cut(s)	342
HaeII	RGCGCY	1 cut(s)	347
HhaI	GCGC	1 cut(s)	346
Hin1I	GRCGYC	1 cut(s)	344
Hin1II	CATG	5 cut(s)	335, 341, 497, 596, 901
Hin6I	GCGC	1 cut(s)	344
HinP1I	GCGC	1 cut(s)	344
HincII	GTYRAC	1 cut(s)	71
HindII	GTYRAC	1 cut(s)	71
HinfI	GANTC	6 cut(s)	197, 308, 646, 710, 884, 962
HphI	GGTGA	3 cut(s)	302, 829, 834
Hpy166II	GTNNAC	2 cut(s)	59, 71
Hpy188I	TCNGA	7 cut(s)	81, 202, 220, 361, 557, 679, 873
Hpy188III	TCNNGA	7 cut(s)	206, 494, 543, 608, 688, 898, 973
Hpy8I	GTNNAC	2 cut(s)	59, 71
Hpy99I	CGWCG	1 cut(s)	941
HpyAV	CCTTC	3 cut(s)	128, 187, 415
HpyCH4III	ACNGT	4 cut(s)	272, 624, 746, 846
HpyCH4IV	ACGT	1 cut(s)	356
HpyCH4V	TGCA	5 cut(s)	305, 561, 579, 598, 657
HpyF10VI	GCNNNNNNNGC	1 cut(s)	347
HpyF3I	CTNAG	3 cut(s)	74, 117, 372
HpySE526I	ACGT	1 cut(s)	356
Hsp92I	GRCGYC	1 cut(s)	344
Hsp92II	CATG	5 cut(s)	335, 341, 497, 596, 901
HspAI	GCGC	1 cut(s)	344
KasI	GGCGCC	1 cut(s)	343
Ksp22I	TGATCA	4 cut(s)	202, 220, 808, 850
LguI	GCTCTTC	1 cut(s)	756
LmnI	GCTCC	1 cut(s)	376
LpnPI	CCDG	4 cut(s)	47, 360, 667, 876
LweI	GCATC	3 cut(s)	313, 548, 927
MaeI	CTAG	2 cut(s)	462, 705
MaeII	ACGT	1 cut(s)	356
MaeIII	GTNAC	2 cut(s)	266, 824
MbiI	CCGCTC	1 cut(s)	903
MboII	GAAGA	6 cut(s)	374, 447, 705, 773, 803, 879
MhlI	GDGCHC	2 cut(s)	398, 768
MluCI	AATT	6 cut(s)	225, 518, 569, 603, 680, 691
Mly113I	GGCGCC	1 cut(s)	344
MlyI	GAGTC	3 cut(s)	206, 893, 971
MmeI	TCCRAC	1 cut(s)	59
MnlI	CCTC	5 cut(s)	31, 112, 367, 413, 934
MseI	TTAA	3 cut(s)	144, 669, 786
MslI	CAYNNNNRTG	2 cut(s)	336, 844
Mva1269I	GAATGC	1 cut(s)	538
MwoI	GCNNNNNNNGC	1 cut(s)	347
NarI	GGCGCC	1 cut(s)	344
NdeI	CATATG	1 cut(s)	813
NlaIII	CATG	5 cut(s)	335, 341, 497, 596, 901
NlaIV	GGNNCC	1 cut(s)	345
NmuCI	GTSAC	2 cut(s)	266, 824
NspI	RCATGY	1 cut(s)	596
NspV	TTCGAA	1 cut(s)	628
PagI	TCATGA	2 cut(s)	493, 897
PaqCI	CACCTGC	1 cut(s)	662
PciI	ACATGT	1 cut(s)	592
PciSI	GCTCTTC	1 cut(s)	756
PctI	GAATGC	1 cut(s)	538
PfeI	GAWTC	3 cut(s)	308, 646, 710
PkrI	GCNGC	1 cut(s)	343
PleI	GAGTC	3 cut(s)	205, 892, 970
PluTI	GGCGCC	1 cut(s)	347
PpsI	GAGTC	3 cut(s)	205, 892, 970
PscI	ACATGT	1 cut(s)	592
PsiI	TTATAA	1 cut(s)	185
PspN4I	GGNNCC	1 cut(s)	345
RsaI	GTAC	1 cut(s)	509
RsaNI	GTAC	1 cut(s)	508
RseI	CAYNNNNRTG	2 cut(s)	336, 844
SapI	GCTCTTC	1 cut(s)	756
SaqAI	TTAA	3 cut(s)	144, 669, 786
SatI	GCNGC	1 cut(s)	342
SchI	GAGTC	3 cut(s)	206, 893, 971
SduI	GDGCHC	2 cut(s)	398, 768
SetI	ASST	7 cut(s)	98, 139, 359, 407, 424, 656, 669
SfaNI	GCATC	3 cut(s)	313, 548, 927
SfoI	GGCGCC	1 cut(s)	345
SfuI	TTCGAA	1 cut(s)	628
SmiMI	CAYNNNNRTG	2 cut(s)	336, 844
Sse9I	AATT	6 cut(s)	225, 518, 569, 603, 680, 691
SsiI	CCGC	4 cut(s)	300, 341, 696, 903
SspDI	GGCGCC	1 cut(s)	343
SspMI	CTAG	2 cut(s)	462, 705
StyI	CCWWGG	1 cut(s)	49
TaaI	ACNGT	4 cut(s)	272, 624, 746, 846
TaiI	ACGT	1 cut(s)	359
TaqI	TCGA	6 cut(s)	573, 628, 863, 921, 960, 972
TasI	AATT	6 cut(s)	225, 518, 569, 603, 680, 691
TauI	GCSGC	1 cut(s)	344
TfiI	GAWTC	3 cut(s)	308, 646, 710
Tru1I	TTAA	3 cut(s)	144, 669, 786
Tru9I	TTAA	3 cut(s)	144, 669, 786
TscAI	CASTG	2 cut(s)	262, 627
TseFI	GTSAC	2 cut(s)	266, 824
Tsp45I	GTSAC	2 cut(s)	266, 824
TspDTI	ATGAA	6 cut(s)	300, 326, 510, 712, 828, 993
TspGWI	ACGGA	1 cut(s)	525
TspRI	CASTG	2 cut(s)	262, 627
XapI	RAATTY	5 cut(s)	225, 518, 569, 603, 691
XceI	RCATGY	1 cut(s)	596
XspI	CTAG	2 cut(s)	462, 705

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.