Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr3B

Orthogroup ID

OG0001288

Physical Location & Seq

Genomic Coordinates Forward (+)

7549287 .. 7569828

Gene Structure 20542 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh3BG094800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 855 bp

ATGGAAGAAGAGCTGGTTCGAGGGGAGGAATTTGCGAATCAACTTCGCAAGTTTCTTGATGATCATGGAGATCATGGTCGATGGGTGGTGGCCCAAGCTCTTGGTACGAAGATCTCGAGCTCTTTTACGAATTCGAATCGCCTTTCAATCTTAAAAGGGAATCATCAGGGGGGGCTAGTTCCTCATATTCGAGCCAACACTACTTTTGCTGCGCCTACTATTGTGAATTCATCGTCTTGGAATGCACAGGAGGGACGACATAGGGCTATTATGAGTACCGAAACGTCCAAGGACCGTAGAGGTTGTTATAAGAGAAGAAAGACTCCATATTCATGGACCAGAAACACTCCAGATTTGGTTAACGATGGTCATGCATGGAGAAAGTATGGACAGAAACAGATCCTCAATGCCACCCACCCGAGGAACTACTTTAGGTGCAGCCATAAGTACGATCAAGGCTGCAGAGCAACCAAGCAGTGGAACCACTCCCTCAAGTTCACGCTCGACAACGCCGCTCTCAACCAGGACCACCTCAAAGTCGAGATTCAGCTCGTCTCGGACCGCACCCTCCGAGACACCAAGATCGGAAAAGTCGTTATTCCGGCAAAGGAGTTGCTTCAGTATTCCGGCACGGGCGTGAGCTACCAGCTTGTGACGCGCAAGGGCAGATCCAAAGGGAGCGTCAGCTTCAGGTACAAGTTGGGCGAGAAATACTGGGTTGAGCCCGAGCCAAAACCCGAGCCCGTCATGGCTTACCCCGCCGCGGGTTATGGAAACCCGCCAGTTGTGCAGCGGCCGAAGAAGTCCAAAAAAGGAAAAGTGACGCTTGTGGCTACTACCACCCAAGACAATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0002218 GO:0002252 GO:0002253 GO:0002376 GO:0002682 GO:0002684 GO:0003674 GO:0003700 GO:0006355 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009627 GO:0009682 GO:0009719 GO:0009725 GO:0009751 GO:0009753 GO:0009755 GO:0009814 GO:0009862 GO:0009863 GO:0009864 GO:0009867 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010200 GO:0010243 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0014070 GO:0019219 GO:0019222 GO:0023052 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031347 GO:0031349 GO:0032870 GO:0035690 GO:0042221 GO:0042493 GO:0042742 GO:0043207 GO:0045087 GO:0045088 GO:0045089 GO:0045892 GO:0045934 GO:0046677 GO:0048518 GO:0048519 GO:0048523 GO:0048583 GO:0048584 GO:0048831 GO:0050776 GO:0050778 GO:0050789 GO:0050793 GO:0050794 GO:0050832 GO:0050896 GO:0051093 GO:0051171 GO:0051172 GO:0051239 GO:0051241 GO:0051252 GO:0051253 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0065007 GO:0070887 GO:0071229 GO:0071236 GO:0071310 GO:0071395 GO:0071407 GO:0071446 GO:0071495 GO:0080090 GO:0080134 GO:0098542 GO:0140110 GO:1900055 GO:1900056 GO:1901698 GO:1901700 GO:1901701 GO:1902679 GO:1903506 GO:1903507 GO:1905622 GO:2000024 GO:2000026 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WRKY

Protein Analysis

284

Amino Acids

32.22

Weight (kDa)

10.0

Isoelectric Point (pI)

35.64

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WRKY	PF03106	121 - 163	8.7e-16	WRKY DNA -binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001288)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G22570
fragaria_vesca	FvH4_6g09650 FvH4_6g09710 FvH4_6g09710 FvH4_6g09730 FvH4_6g09740 FvH4_6g09740 FvH4_6g09740 FvH4_6g09740 FvH4_6g09740 FvH4_6g09740 FvH4_6g09750 FvH4_6g09760
malus_domestica	MD04G1175500.v1.1 MD04G1175600.v1.1 MD12G1189200.v1.1 MD12G1189400.v1.1 MD12G1189600.v1.1 MD12G1189700.v1.1 MD12G1189900.v1.1
prunus_persica	Prupe.6G294900_v2.0.a1 Prupe.6G295000_v2.0.a1 Prupe.6G295100_v2.0.a1
pyrus_communis	pycom04g15480 pycom04g15490 pycom12g17650 pycom12g17690 pycom12g17700 pycom12g17730
rosa_chinensis	RchiOBHm_Chr3g0460351 RchiOBHm_Chr3g0460361
rosa_laevigata	RLG00000025018 RLG00000025019 RLG00000025027 RLG00000025113
rosa_multiflora	Rmu_sc0000449.1_g000005 Rmu_sc0004267.1_g000019
rosa_roxburghii	Rroxscaffold_6G00419560 Rroxscaffold_6G00419590
rosa_rugosa	Rorug03G0033500 Rorug03G0033500 Rorug03G0041400 Rorug03G0041800 Rorug03G0042000
rosa_samantha	Rh3AG100000 Rh3AG100100 Rh3BG094800 Rh3BG103900 Rh3BG104000 Rh3CG105700 Rh3CG105800 Rh3DG105300 Rh3DG105400
rosa_wichuraiana	Rw3G008480 Rw3G008490

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	309
AccB7I	CCANNNNNTGG	1 cut(s)	477
AccBSI	CCGCTC	1 cut(s)	515
AccII	CGCG	2 cut(s)	658, 764
AciI	CCGC	7 cut(s)	513, 562, 759, 762, 764, 779, 793
AclWI	GGATC	2 cut(s)	394, 663
AcoI	YGGCCR	1 cut(s)	794
AcsI	RAATTY	3 cut(s)	29, 130, 226
AcuI	CTGAAG	2 cut(s)	602, 673
AfaI	GTAC	4 cut(s)	106, 277, 449, 695
AfiI	CCNNNNNNNGG	4 cut(s)	420, 477, 763, 764
AgsI	TTSAA	1 cut(s)	147
AjnI	CCWGG	1 cut(s)	522
AleI	CACNNNNGTG	1 cut(s)	635
AluBI	AGCT	7 cut(s)	13, 98, 120, 550, 642, 649, 687
AluI	AGCT	7 cut(s)	13, 98, 120, 550, 642, 649, 687
Alw21I	GWGCWC	1 cut(s)	122
Alw26I	GTCTC	2 cut(s)	559, 567
AlwI	GGATC	2 cut(s)	394, 663
Ama87I	CYCGRG	4 cut(s)	115, 418, 725, 737
AoxI	GGCC	2 cut(s)	90, 794
ApeKI	GCWGC	4 cut(s)	209, 438, 459, 790
ApoI	RAATTY	3 cut(s)	29, 130, 226
ArsI	GACNNNNNNTTYG	2 cut(s)	666, 698
AspLEI	GCGC	2 cut(s)	214, 660
AspS9I	GGNCC	5 cut(s)	91, 292, 336, 526, 559
AsuII	TTCGAA	1 cut(s)	134
AvaI	CYCGRG	4 cut(s)	115, 418, 725, 737
AvaII	GGWCC	4 cut(s)	292, 336, 526, 559
BanII	GRGCYC	3 cut(s)	122, 726, 744
Bbv12I	GWGCWC	1 cut(s)	122
BbvI	GCAGC	4 cut(s)	196, 446, 450, 802
BccI	CCATC	2 cut(s)	75, 359
BcgI	CGANNNNNNTGC	2 cut(s)	353, 387
BciT130I	CCWGG	1 cut(s)	524
BclI	TGATCA	1 cut(s)	61
BcoDI	GTCTC	2 cut(s)	559, 567
BfaI	CTAG	1 cut(s)	176
BfmI	CTRYAG	1 cut(s)	460
BglII	AGATCT	1 cut(s)	111
BisI	GCNGC	7 cut(s)	210, 439, 460, 513, 762, 791, 794
BlsI	GCNGC	7 cut(s)	211, 440, 461, 514, 763, 792, 795
Bme1390I	CCNGG	1 cut(s)	524
Bme18I	GGWCC	4 cut(s)	292, 336, 526, 559
BmeT110I	CYCGRG	4 cut(s)	115, 418, 725, 737
BmgT120I	GGNCC	5 cut(s)	91, 292, 336, 526, 559
BmiI	GGNNCC	1 cut(s)	482
BmrFI	CCNGG	1 cut(s)	524
BmrI	ACTGGG	1 cut(s)	724
BmuI	ACTGGG	1 cut(s)	724
BpmI	CTGGAG	1 cut(s)	333
Bpu14I	TTCGAA	1 cut(s)	134
BpuEI	CTTGAG	1 cut(s)	476
BsaJI	CCNNGG	3 cut(s)	288, 419, 762
Bsc4I	CCNNNNNNNGG	4 cut(s)	420, 477, 763, 764
Bse1I	ACTGG	2 cut(s)	719, 782
BseBI	CCWGG	1 cut(s)	524
BseDI	CCNNGG	3 cut(s)	288, 419, 762
BseLI	CCNNNNNNNGG	4 cut(s)	420, 477, 763, 764
BseNI	ACTGG	2 cut(s)	719, 782
BseX3I	CGGCCG	1 cut(s)	794
BseXI	GCAGC	4 cut(s)	196, 446, 450, 802
BsgI	GTGCAG	2 cut(s)	457, 809
Bsh1236I	CGCG	2 cut(s)	658, 764
Bsh1285I	CGRYCG	1 cut(s)	797
BshFI	GGCC	2 cut(s)	92, 796
BsiEI	CGRYCG	1 cut(s)	797
BsiHKAI	GWGCWC	1 cut(s)	122
BsiHKCI	CYCGRG	4 cut(s)	115, 418, 725, 737
BsiSI	CCGG	2 cut(s)	602, 627
BslFI	GGGAC	1 cut(s)	267
BslI	CCNNNNNNNGG	4 cut(s)	420, 477, 763, 764
BsmAI	GTCTC	2 cut(s)	559, 567
BsmBI	CGTCTC	1 cut(s)	559
BsmFI	GGGAC	1 cut(s)	267
BsmI	GAATGC	1 cut(s)	247
BsnI	GGCC	2 cut(s)	92, 796
BsoBI	CYCGRG	4 cut(s)	115, 418, 725, 737
Bsp119I	TTCGAA	1 cut(s)	134
Bsp1286I	GDGCHC	3 cut(s)	122, 726, 744
Bsp143I	GATC	7 cut(s)	61, 70, 111, 399, 451, 582, 668
BspACI	CCGC	7 cut(s)	513, 562, 759, 762, 764, 779, 793
BspANI	GGCC	2 cut(s)	92, 796
BspFNI	CGCG	2 cut(s)	658, 764
BspLI	GGNNCC	1 cut(s)	482
BspMAI	CTGCAG	1 cut(s)	464
BspPI	GGATC	2 cut(s)	394, 663
BspQI	GCTCTTC	1 cut(s)	3
BspT104I	TTCGAA	1 cut(s)	134
BsrBI	CCGCTC	1 cut(s)	515
BsrI	ACTGG	2 cut(s)	719, 782
BssECI	CCNNGG	3 cut(s)	288, 419, 762
BssMI	GATC	7 cut(s)	61, 70, 111, 399, 451, 582, 668
BssT1I	CCWWGG	1 cut(s)	288
Bst2UI	CCWGG	1 cut(s)	524
Bst4CI	ACNGT	1 cut(s)	296
Bst6I	CTCTTC	1 cut(s)	3
BstBI	TTCGAA	1 cut(s)	134
BstDSI	CCRYGG	1 cut(s)	762
BstFNI	CGCG	2 cut(s)	658, 764
BstHHI	GCGC	2 cut(s)	214, 660
BstKTI	GATC	7 cut(s)	64, 73, 114, 402, 454, 585, 671
BstMAI	GTCTC	2 cut(s)	559, 567
BstMBI	GATC	7 cut(s)	61, 70, 111, 399, 451, 582, 668
BstMCI	CGRYCG	1 cut(s)	797
BstMWI	GCNNNNNNNGC	3 cut(s)	655, 758, 787
BstNI	CCWGG	1 cut(s)	524
BstSCI	CCNGG	1 cut(s)	522
BstSFI	CTRYAG	1 cut(s)	460
BstUI	CGCG	2 cut(s)	658, 764
BstV1I	GCAGC	4 cut(s)	196, 446, 450, 802
BstX2I	RGATCY	3 cut(s)	111, 399, 668
BstXI	CCANNNNNNTGG	2 cut(s)	101, 333
BstYI	RGATCY	3 cut(s)	111, 399, 668
BstZI	CGGCCG	1 cut(s)	794
BsuRI	GGCC	2 cut(s)	92, 796
BtgI	CCRYGG	1 cut(s)	762
BtsI	GCAGTG	1 cut(s)	482
BtsIMutI	CAGTG	1 cut(s)	482
CfoI	GCGC	2 cut(s)	214, 660
Cfr13I	GGNCC	5 cut(s)	91, 292, 336, 526, 559
Cfr42I	CCGCGG	1 cut(s)	765
CpoI	CGGWCCG	1 cut(s)	559
CseI	GACGC	3 cut(s)	664, 670, 832
Csp6I	GTAC	4 cut(s)	105, 276, 448, 694
CspI	CGGWCCG	1 cut(s)	559
CviAII	CATG	6 cut(s)	65, 74, 333, 371, 375, 748
CviQI	GTAC	4 cut(s)	105, 276, 448, 694
DpnI	GATC	7 cut(s)	63, 72, 113, 401, 453, 584, 670
DpnII	GATC	7 cut(s)	61, 70, 111, 399, 451, 582, 668
EaeI	YGGCCR	1 cut(s)	794
EagI	CGGCCG	1 cut(s)	794
Eam1104I	CTCTTC	1 cut(s)	3
EarI	CTCTTC	1 cut(s)	3
Ecl136II	GAGCTC	1 cut(s)	120
EclXI	CGGCCG	1 cut(s)	794
Eco130I	CCWWGG	1 cut(s)	288
Eco24I	GRGCYC	3 cut(s)	122, 726, 744
Eco47I	GGWCC	4 cut(s)	292, 336, 526, 559
Eco52I	CGGCCG	1 cut(s)	794
Eco53kI	GAGCTC	1 cut(s)	120
Eco57I	CTGAAG	2 cut(s)	602, 673
Eco88I	CYCGRG	4 cut(s)	115, 418, 725, 737
EcoICRI	GAGCTC	1 cut(s)	120
EcoRI	GAATTC	2 cut(s)	130, 226
EcoRII	CCWGG	1 cut(s)	522
EcoT14I	CCWWGG	1 cut(s)	288
EcoT22I	ATGCAT	1 cut(s)	376
EcoT38I	GRGCYC	3 cut(s)	122, 726, 744
ErhI	CCWWGG	1 cut(s)	288
Esp3I	CGTCTC	1 cut(s)	559
FaeI	CATG	6 cut(s)	68, 77, 336, 374, 378, 751
FalI	AAGNNNNNCTT	2 cut(s)	810, 842
FaqI	GGGAC	1 cut(s)	267
FatI	CATG	6 cut(s)	64, 73, 332, 370, 374, 747
FauI	CCCGC	3 cut(s)	757, 766, 786
FbaI	TGATCA	1 cut(s)	61
Fnu4HI	GCNGC	7 cut(s)	210, 439, 460, 513, 762, 791, 794
FriOI	GRGCYC	3 cut(s)	122, 726, 744
Fsp4HI	GCNGC	7 cut(s)	210, 439, 460, 513, 762, 791, 794
FspBI	CTAG	1 cut(s)	176
GlaI	GCGC	2 cut(s)	213, 659
GluI	GCNGC	7 cut(s)	210, 439, 460, 513, 762, 791, 794
GsuI	CTGGAG	1 cut(s)	333
HaeIII	GGCC	2 cut(s)	92, 796
HapII	CCGG	2 cut(s)	602, 627
HgaI	GACGC	3 cut(s)	664, 670, 832
HhaI	GCGC	2 cut(s)	214, 660
Hin1II	CATG	6 cut(s)	68, 77, 336, 374, 378, 751
Hin6I	GCGC	2 cut(s)	212, 658
HinP1I	GCGC	2 cut(s)	212, 658
HincII	GTYRAC	1 cut(s)	361
HindII	GTYRAC	1 cut(s)	361
HinfI	GANTC	5 cut(s)	37, 136, 160, 322, 544
HpaI	GTTAAC	1 cut(s)	361
HpaII	CCGG	2 cut(s)	602, 627
Hpy166II	GTNNAC	2 cut(s)	361, 498
Hpy188I	TCNGA	3 cut(s)	559, 572, 587
Hpy188III	TCNNGA	4 cut(s)	56, 115, 350, 541
Hpy8I	GTNNAC	2 cut(s)	361, 498
HpyCH4III	ACNGT	1 cut(s)	296
HpyCH4IV	ACGT	1 cut(s)	284
HpyCH4V	TGCA	5 cut(s)	245, 374, 438, 462, 790
HpyF10VI	GCNNNNNNNGC	3 cut(s)	655, 758, 787
HpySE526I	ACGT	1 cut(s)	284
Hsp92II	CATG	6 cut(s)	68, 77, 336, 374, 378, 751
HspAI	GCGC	2 cut(s)	212, 658
Ksp22I	TGATCA	1 cut(s)	61
KspAI	GTTAAC	1 cut(s)	361
KspI	CCGCGG	1 cut(s)	765
Kzo9I	GATC	7 cut(s)	61, 70, 111, 399, 451, 582, 668
LguI	GCTCTTC	1 cut(s)	3
LmnI	GCTCC	1 cut(s)	678
Lsp1109I	GCAGC	4 cut(s)	196, 446, 450, 802
MaeI	CTAG	1 cut(s)	176
MaeII	ACGT	1 cut(s)	284
MaeIII	GTNAC	2 cut(s)	652, 820
MalI	GATC	7 cut(s)	63, 72, 113, 401, 453, 584, 670
MbiI	CCGCTC	1 cut(s)	515
MboI	GATC	7 cut(s)	61, 70, 111, 399, 451, 582, 668
MboII	GAAGA	5 cut(s)	17, 20, 121, 327, 811
MflI	RGATCY	3 cut(s)	111, 399, 668
MhlI	GDGCHC	3 cut(s)	122, 726, 744
MluCI	AATT	4 cut(s)	29, 130, 226, 850
MlyI	GAGTC	1 cut(s)	316
Mph1103I	ATGCAT	1 cut(s)	376
MseI	TTAA	3 cut(s)	152, 360, 853
MslI	CAYNNNNRTG	2 cut(s)	331, 635
MspA1I	CMGCKG	2 cut(s)	764, 793
MspI	CCGG	2 cut(s)	602, 627
MspR9I	CCNGG	1 cut(s)	524
Mva1269I	GAATGC	1 cut(s)	247
MvaI	CCWGG	1 cut(s)	524
MvnI	CGCG	2 cut(s)	658, 764
MwoI	GCNNNNNNNGC	3 cut(s)	655, 758, 787
NdeII	GATC	7 cut(s)	61, 70, 111, 399, 451, 582, 668
NlaIII	CATG	6 cut(s)	68, 77, 336, 374, 378, 751
NlaIV	GGNNCC	1 cut(s)	482
NmuCI	GTSAC	2 cut(s)	652, 820
NsiI	ATGCAT	1 cut(s)	376
NspV	TTCGAA	1 cut(s)	134
OliI	CACNNNNGTG	1 cut(s)	635
PaeR7I	CTCGAG	1 cut(s)	115
PciSI	GCTCTTC	1 cut(s)	3
PcsI	WCGNNNNNNNCGW	2 cut(s)	113, 591
PctI	GAATGC	1 cut(s)	247
PfeI	GAWTC	4 cut(s)	37, 136, 160, 544
PflMI	CCANNNNNTGG	1 cut(s)	477
PkrI	GCNGC	7 cut(s)	211, 440, 461, 514, 763, 792, 795
PleI	GAGTC	1 cut(s)	316
PpsI	GAGTC	1 cut(s)	316
PsiI	TTATAA	1 cut(s)	309
Psp124BI	GAGCTC	1 cut(s)	122
Psp6I	CCWGG	1 cut(s)	522
PspGI	CCWGG	1 cut(s)	522
PspN4I	GGNNCC	1 cut(s)	482
PspPI	GGNCC	5 cut(s)	91, 292, 336, 526, 559
PstI	CTGCAG	1 cut(s)	464
PsuI	RGATCY	3 cut(s)	111, 399, 668
RsaI	GTAC	4 cut(s)	106, 277, 449, 695
RsaNI	GTAC	4 cut(s)	105, 276, 448, 694
RseI	CAYNNNNRTG	2 cut(s)	331, 635
Rsr2I	CGGWCCG	1 cut(s)	559
RsrII	CGGWCCG	1 cut(s)	559
SacI	GAGCTC	1 cut(s)	122
SacII	CCGCGG	1 cut(s)	765
SapI	GCTCTTC	1 cut(s)	3
SaqAI	TTAA	3 cut(s)	152, 360, 853
SatI	GCNGC	7 cut(s)	210, 439, 460, 513, 762, 791, 794
Sau3AI	GATC	7 cut(s)	61, 70, 111, 399, 451, 582, 668
Sau96I	GGNCC	5 cut(s)	91, 292, 336, 526, 559
SchI	GAGTC	1 cut(s)	316
ScrFI	CCNGG	1 cut(s)	524
SduI	GDGCHC	3 cut(s)	122, 726, 744
SfcI	CTRYAG	1 cut(s)	460
Sfr274I	CTCGAG	1 cut(s)	115
Sfr303I	CCGCGG	1 cut(s)	765
SfuI	TTCGAA	1 cut(s)	134
SgrBI	CCGCGG	1 cut(s)	765
SinI	GGWCC	4 cut(s)	292, 336, 526, 559
SlaI	CTCGAG	1 cut(s)	115
SmiMI	CAYNNNNRTG	2 cut(s)	331, 635
SmlI	CTYRAG	2 cut(s)	115, 491
SmoI	CTYRAG	2 cut(s)	115, 491
Sse9I	AATT	4 cut(s)	29, 130, 226, 850
SsiI	CCGC	7 cut(s)	513, 562, 759, 762, 764, 779, 793
SspMI	CTAG	1 cut(s)	176
SstI	GAGCTC	1 cut(s)	122
StyD4I	CCNGG	1 cut(s)	522
StyI	CCWWGG	1 cut(s)	288
TaaI	ACNGT	1 cut(s)	296
TaiI	ACGT	1 cut(s)	287
TaqI	TCGA	7 cut(s)	19, 79, 116, 134, 190, 504, 540
TasI	AATT	4 cut(s)	29, 130, 226, 850
TauI	GCSGC	3 cut(s)	515, 764, 796
TfiI	GAWTC	4 cut(s)	37, 136, 160, 544
Tru1I	TTAA	3 cut(s)	152, 360, 853
Tru9I	TTAA	3 cut(s)	152, 360, 853
TscAI	CASTG	1 cut(s)	482
TseFI	GTSAC	2 cut(s)	652, 820
TseI	GCWGC	4 cut(s)	209, 438, 459, 790
Tsp45I	GTSAC	2 cut(s)	652, 820
TspDTI	ATGAA	2 cut(s)	219, 321
TspRI	CASTG	1 cut(s)	482
Van91I	CCANNNNNTGG	1 cut(s)	477
VpaK11BI	GGWCC	4 cut(s)	292, 336, 526, 559
XapI	RAATTY	3 cut(s)	29, 130, 226
XhoI	CTCGAG	1 cut(s)	115
XspI	CTAG	1 cut(s)	176
Zsp2I	ATGCAT	1 cut(s)	376

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.