Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0001288

Physical Location & Seq

Genomic Coordinates Reverse (-)

27224756 .. 27226237

Gene Structure 1482 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1189900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 714 bp

ATGATGTTAGTGGTGGTGGTGGTGGTGCAGATTCATCATCTTGGGTTGGTAATCATGATCATGCAGCTGTTATTAAGTCTGAAGATTATACTGAGGAGGAGATCAGTTGTAAGAGCACTTCAACCTTCAAGGATCGTAGAGACTTCACACTCTTGGACAAGAGACATTCCTGCTTTGATTGCCGACGGTCATGCATGGAGAAAGTATGGACAAAAAAATATCCACAATGCCAAGCATCCAAGGAACTACTTTAGATGCACTCACAAATACGATCAAGCGTGCAAAGCAACCAAGCACGTGCAACAAGTTCAAGATCATCCATCAGTGTTTCGAACAACATATTATGGCACCCACAGTTGCAGAGACTACCTCAAAACTTCTGAATTGGTCTTGGATTGCACAAATCCTAGAGATTCTTCAAAGTTTATTAGTTTTGACAACGTCAATTGTTTTACAAACAAACAAGAGCACCCATTTCCCGCATCCTTCACTTCATCATCAGTTAAAAAAGAACTAGTCGTCAAGGCAACAAGTGATCATACGGTGACAAGCCACCACAACCATTCGCTACCCAGTGATTATCTCGTGTTGCATGATGATCTGATGGAATTCGCGTCCTCTGAGCCCATGGGCGGGTTTTCATCCAGCATTGATCAGTATGATCACGATGATGTGTTTTTGAGGATGTTTGCCGAGTCTGTCTTTGATATATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0002218 GO:0002252 GO:0002253 GO:0002376 GO:0002682 GO:0002684 GO:0003674 GO:0003700 GO:0006355 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009627 GO:0009682 GO:0009719 GO:0009725 GO:0009751 GO:0009753 GO:0009755 GO:0009814 GO:0009862 GO:0009863 GO:0009864 GO:0009867 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010200 GO:0010243 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0014070 GO:0019219 GO:0019222 GO:0023052 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031347 GO:0031349 GO:0032870 GO:0035690 GO:0042221 GO:0042493 GO:0042742 GO:0043207 GO:0045087 GO:0045088 GO:0045089 GO:0045892 GO:0045934 GO:0046677 GO:0048518 GO:0048519 GO:0048523 GO:0048583 GO:0048584 GO:0048831 GO:0050776 GO:0050778 GO:0050789 GO:0050793 GO:0050794 GO:0050832 GO:0050896 GO:0051093 GO:0051171 GO:0051172 GO:0051239 GO:0051241 GO:0051252 GO:0051253 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0065007 GO:0070887 GO:0071229 GO:0071236 GO:0071310 GO:0071395 GO:0071407 GO:0071446 GO:0071495 GO:0080090 GO:0080134 GO:0098542 GO:0140110 GO:1900055 GO:1900056 GO:1901698 GO:1901700 GO:1901701 GO:1902679 GO:1903506 GO:1903507 GO:1905622 GO:2000024 GO:2000026 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WRKY

Protein Analysis

238

Amino Acids

27.26

Weight (kDa)

8.26

Isoelectric Point (pI)

49.67

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WRKY	PF03106	62 - 120	1.2e-22	WRKY DNA -binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

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Orthologous Genes (Group: OG0001288)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G22570
fragaria_vesca	FvH4_6g09650 FvH4_6g09710 FvH4_6g09710 FvH4_6g09730 FvH4_6g09740 FvH4_6g09740 FvH4_6g09740 FvH4_6g09740 FvH4_6g09740 FvH4_6g09740 FvH4_6g09750 FvH4_6g09760
malus_domestica	MD04G1175500.v1.1 MD04G1175600.v1.1 MD12G1189200.v1.1 MD12G1189400.v1.1 MD12G1189600.v1.1 MD12G1189700.v1.1 MD12G1189900.v1.1
prunus_persica	Prupe.6G294900_v2.0.a1 Prupe.6G295000_v2.0.a1 Prupe.6G295100_v2.0.a1
pyrus_communis	pycom04g15480 pycom04g15490 pycom12g17650 pycom12g17690 pycom12g17700 pycom12g17730
rosa_chinensis	RchiOBHm_Chr3g0460351 RchiOBHm_Chr3g0460361
rosa_laevigata	RLG00000025018 RLG00000025019 RLG00000025027 RLG00000025113
rosa_multiflora	Rmu_sc0000449.1_g000005 Rmu_sc0004267.1_g000019
rosa_roxburghii	Rroxscaffold_6G00419560 Rroxscaffold_6G00419590
rosa_rugosa	Rorug03G0033500 Rorug03G0033500 Rorug03G0041400 Rorug03G0041800 Rorug03G0042000
rosa_samantha	Rh3AG100000 Rh3AG100100 Rh3BG094800 Rh3BG103900 Rh3BG104000 Rh3CG105700 Rh3CG105800 Rh3DG105300 Rh3DG105400
rosa_wichuraiana	Rw3G008480 Rw3G008490

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	347
AccII	CGCG	1 cut(s)	614
AciI	CCGC	2 cut(s)	480, 633
AclWI	GGATC	1 cut(s)	140
AcsI	RAATTY	1 cut(s)	608
AcuI	CTGAAG	1 cut(s)	101
AcvI	CACGTG	1 cut(s)	298
AfiI	CCNNNNNNNGG	2 cut(s)	632, 633
AgsI	TTSAA	4 cut(s)	122, 129, 311, 420
AhlI	ACTAGT	1 cut(s)	514
AluBI	AGCT	1 cut(s)	67
AluI	AGCT	1 cut(s)	67
Alw21I	GWGCWC	2 cut(s)	118, 471
Alw26I	GTCTC	3 cut(s)	134, 156, 357
AlwI	GGATC	1 cut(s)	140
ApeKI	GCWGC	1 cut(s)	64
ApoI	RAATTY	1 cut(s)	608
AsuHPI	GGTGA	1 cut(s)	556
AsuII	TTCGAA	1 cut(s)	331
BanI	GGYRCC	1 cut(s)	347
BanII	GRGCYC	1 cut(s)	627
BarI	GAAGNNNNNNTAC	2 cut(s)	102, 134
BauI	CACGAG	1 cut(s)	584
BbrPI	CACGTG	1 cut(s)	298
Bbv12I	GWGCWC	2 cut(s)	118, 471
BbvI	GCAGC	1 cut(s)	76
BccI	CCATC	2 cut(s)	328, 598
BcgI	CGANNNNNNTGC	2 cut(s)	173, 207
BclI	TGATCA	4 cut(s)	57, 535, 652, 661
BcoDI	GTCTC	3 cut(s)	134, 156, 357
BcuI	ACTAGT	1 cut(s)	514
BfaI	CTAG	2 cut(s)	408, 515
BisI	GCNGC	1 cut(s)	65
BlsI	GCNGC	1 cut(s)	66
BmiI	GGNNCC	1 cut(s)	349
BmrI	ACTGGG	1 cut(s)	567
BmsI	GCATC	3 cut(s)	244, 245, 491
BmuI	ACTGGG	1 cut(s)	567
BplI	GAGNNNNNCTC	2 cut(s)	354, 386
Bpu14I	TTCGAA	1 cut(s)	331
BsaAI	YACGTR	1 cut(s)	298
BsaJI	CCNNGG	2 cut(s)	239, 627
Bsc4I	CCNNNNNNNGG	2 cut(s)	632, 633
Bse1I	ACTGG	1 cut(s)	573
BseDI	CCNNGG	2 cut(s)	239, 627
BseGI	GGATG	5 cut(s)	235, 316, 482, 641, 690
BseLI	CCNNNNNNNGG	2 cut(s)	632, 633
BseMII	CTCAG	2 cut(s)	83, 612
BseNI	ACTGG	1 cut(s)	573
BseRI	GAGGAG	2 cut(s)	109, 112
BseXI	GCAGC	1 cut(s)	76
BsgI	GTGCAG	1 cut(s)	47
Bsh1236I	CGCG	1 cut(s)	614
BshNI	GGYRCC	1 cut(s)	347
BsiHKAI	GWGCWC	2 cut(s)	118, 471
BslI	CCNNNNNNNGG	2 cut(s)	632, 633
BsmAI	GTCTC	3 cut(s)	134, 156, 357
Bsp119I	TTCGAA	1 cut(s)	331
Bsp1286I	GDGCHC	3 cut(s)	118, 471, 627
Bsp143I	GATC	9 cut(s)	57, 101, 132, 271, 313, 535, 598, 652, 661
Bsp19I	CCATGG	1 cut(s)	627
BspACI	CCGC	2 cut(s)	480, 633
BspCNI	CTCAG	2 cut(s)	84, 613
BspFNI	CGCG	1 cut(s)	614
BspHI	TCATGA	1 cut(s)	54
BspLI	GGNNCC	1 cut(s)	349
BspPI	GGATC	1 cut(s)	140
BspT104I	TTCGAA	1 cut(s)	331
BspT107I	GGYRCC	1 cut(s)	347
BsrI	ACTGG	1 cut(s)	573
BssECI	CCNNGG	2 cut(s)	239, 627
BssMI	GATC	9 cut(s)	57, 101, 132, 271, 313, 535, 598, 652, 661
BssSI	CACGAG	1 cut(s)	584
BssT1I	CCWWGG	2 cut(s)	239, 627
Bst2BI	CACGAG	1 cut(s)	584
Bst4CI	ACNGT	3 cut(s)	188, 356, 544
BstBAI	YACGTR	1 cut(s)	298
BstBI	TTCGAA	1 cut(s)	331
BstC8I	GCNNGC	1 cut(s)	280
BstDEI	CTNAG	2 cut(s)	92, 621
BstDSI	CCRYGG	1 cut(s)	627
BstF5I	GGATG	5 cut(s)	235, 316, 482, 641, 690
BstFNI	CGCG	1 cut(s)	614
BstKTI	GATC	9 cut(s)	60, 104, 135, 274, 316, 538, 601, 655, 664
BstMAI	GTCTC	3 cut(s)	134, 156, 357
BstMBI	GATC	9 cut(s)	57, 101, 132, 271, 313, 535, 598, 652, 661
BstMWI	GCNNNNNNNGC	2 cut(s)	179, 284
BstUI	CGCG	1 cut(s)	614
BstV1I	GCAGC	1 cut(s)	76
BtgI	CCRYGG	1 cut(s)	627
BtsCI	GGATG	5 cut(s)	235, 316, 482, 641, 690
BtsIMutI	CAGTG	2 cut(s)	330, 580
Cac8I	GCNNGC	1 cut(s)	280
CciI	TCATGA	1 cut(s)	54
CseI	GACGC	1 cut(s)	603
CviAII	CATG	6 cut(s)	55, 61, 191, 195, 593, 628
CviJI	RGCY	3 cut(s)	67, 552, 625
CviKI_1	RGCY	3 cut(s)	67, 552, 625
DdeI	CTNAG	2 cut(s)	92, 621
DpnI	GATC	9 cut(s)	59, 103, 134, 273, 315, 537, 600, 654, 663
DpnII	GATC	9 cut(s)	57, 101, 132, 271, 313, 535, 598, 652, 661
Eco130I	CCWWGG	2 cut(s)	239, 627
Eco24I	GRGCYC	1 cut(s)	627
Eco57I	CTGAAG	1 cut(s)	101
Eco72I	CACGTG	1 cut(s)	298
EcoRI	GAATTC	1 cut(s)	608
EcoT14I	CCWWGG	2 cut(s)	239, 627
EcoT22I	ATGCAT	1 cut(s)	196
EcoT38I	GRGCYC	1 cut(s)	627
ErhI	CCWWGG	2 cut(s)	239, 627
FaeI	CATG	6 cut(s)	58, 64, 194, 198, 596, 631
FatI	CATG	6 cut(s)	54, 60, 190, 194, 592, 627
FauI	CCCGC	2 cut(s)	487, 626
FbaI	TGATCA	4 cut(s)	57, 535, 652, 661
Fnu4HI	GCNGC	1 cut(s)	65
FokI	GGATG	5 cut(s)	222, 303, 469, 628, 697
FriOI	GRGCYC	1 cut(s)	627
Fsp4HI	GCNGC	1 cut(s)	65
FspBI	CTAG	2 cut(s)	408, 515
GluI	GCNGC	1 cut(s)	65
HgaI	GACGC	1 cut(s)	603
Hin1II	CATG	6 cut(s)	58, 64, 194, 198, 596, 631
HinfI	GANTC	3 cut(s)	31, 413, 695
HphI	GGTGA	1 cut(s)	556
Hpy188I	TCNGA	4 cut(s)	81, 382, 603, 622
Hpy188III	TCNNGA	3 cut(s)	55, 311, 665
Hpy99I	CGWCG	1 cut(s)	188
HpyAV	CCTTC	2 cut(s)	135, 496
HpyCH4III	ACNGT	3 cut(s)	188, 356, 544
HpyCH4IV	ACGT	2 cut(s)	297, 441
HpyCH4V	TGCA	9 cut(s)	28, 64, 194, 258, 282, 301, 360, 399, 592
HpyF10VI	GCNNNNNNNGC	2 cut(s)	179, 284
HpyF3I	CTNAG	2 cut(s)	92, 621
HpySE526I	ACGT	2 cut(s)	297, 441
Hsp92II	CATG	6 cut(s)	58, 64, 194, 198, 596, 631
Ksp22I	TGATCA	4 cut(s)	57, 535, 652, 661
Kzo9I	GATC	9 cut(s)	57, 101, 132, 271, 313, 535, 598, 652, 661
LpnPI	CCDG	3 cut(s)	183, 586, 658
Lsp1109I	GCAGC	1 cut(s)	76
LweI	GCATC	3 cut(s)	244, 245, 491
MaeI	CTAG	2 cut(s)	408, 515
MaeII	ACGT	2 cut(s)	297, 441
MaeIII	GTNAC	1 cut(s)	544
MalI	GATC	9 cut(s)	59, 103, 134, 273, 315, 537, 600, 654, 663
MboI	GATC	9 cut(s)	57, 101, 132, 271, 313, 535, 598, 652, 661
MboII	GAAGA	2 cut(s)	94, 408
MfeI	CAATTG	1 cut(s)	445
MhlI	GDGCHC	3 cut(s)	118, 471, 627
MluCI	AATT	3 cut(s)	383, 445, 608
MlyI	GAGTC	1 cut(s)	704
MnlI	CCTC	5 cut(s)	87, 90, 380, 628, 675
Mph1103I	ATGCAT	1 cut(s)	196
MseI	TTAA	2 cut(s)	74, 504
MslI	CAYNNNNRTG	2 cut(s)	59, 669
MspA1I	CMGCKG	1 cut(s)	67
MunI	CAATTG	1 cut(s)	445
MvnI	CGCG	1 cut(s)	614
MwoI	GCNNNNNNNGC	2 cut(s)	179, 284
NcoI	CCATGG	1 cut(s)	627
NdeII	GATC	9 cut(s)	57, 101, 132, 271, 313, 535, 598, 652, 661
NlaIII	CATG	6 cut(s)	58, 64, 194, 198, 596, 631
NlaIV	GGNNCC	1 cut(s)	349
NmuCI	GTSAC	1 cut(s)	544
NsiI	ATGCAT	1 cut(s)	196
NspV	TTCGAA	1 cut(s)	331
PagI	TCATGA	1 cut(s)	54
PfeI	GAWTC	2 cut(s)	31, 413
PflFI	GACNNNGTC	1 cut(s)	440
PkrI	GCNGC	1 cut(s)	66
PleI	GAGTC	1 cut(s)	703
PmaCI	CACGTG	1 cut(s)	298
PmlI	CACGTG	1 cut(s)	298
PpsI	GAGTC	1 cut(s)	703
Ppu21I	YACGTR	1 cut(s)	298
PspCI	CACGTG	1 cut(s)	298
PspN4I	GGNNCC	1 cut(s)	349
PsyI	GACNNNGTC	1 cut(s)	440
PvuII	CAGCTG	1 cut(s)	67
RseI	CAYNNNNRTG	2 cut(s)	59, 669
SaqAI	TTAA	2 cut(s)	74, 504
SatI	GCNGC	1 cut(s)	65
Sau3AI	GATC	9 cut(s)	57, 101, 132, 271, 313, 535, 598, 652, 661
SchI	GAGTC	1 cut(s)	704
SduI	GDGCHC	3 cut(s)	118, 471, 627
SetI	ASST	5 cut(s)	69, 127, 300, 372, 444
SfaNI	GCATC	3 cut(s)	244, 245, 491
SfuI	TTCGAA	1 cut(s)	331
SmiMI	CAYNNNNRTG	2 cut(s)	59, 669
SpeI	ACTAGT	1 cut(s)	514
Sse9I	AATT	3 cut(s)	383, 445, 608
SsiI	CCGC	2 cut(s)	480, 633
SspMI	CTAG	2 cut(s)	408, 515
StyI	CCWWGG	2 cut(s)	239, 627
TaaI	ACNGT	3 cut(s)	188, 356, 544
TaiI	ACGT	2 cut(s)	300, 444
TaqI	TCGA	1 cut(s)	331
TasI	AATT	3 cut(s)	383, 445, 608
TfiI	GAWTC	2 cut(s)	31, 413
Tru1I	TTAA	2 cut(s)	74, 504
Tru9I	TTAA	2 cut(s)	74, 504
TscAI	CASTG	2 cut(s)	330, 580
TseFI	GTSAC	1 cut(s)	544
TseI	GCWGC	1 cut(s)	64
Tsp45I	GTSAC	1 cut(s)	544
TspDTI	ATGAA	3 cut(s)	23, 483, 630
TspRI	CASTG	2 cut(s)	330, 580
Tth111I	GACNNNGTC	1 cut(s)	440
XapI	RAATTY	1 cut(s)	608
XspI	CTAG	2 cut(s)	408, 515
Zsp2I	ATGCAT	1 cut(s)	196

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.