Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr3A

Orthogroup ID

OG0001288

Physical Location & Seq

Genomic Coordinates Forward (+)

8345268 .. 8347302

Gene Structure 2035 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh3AG100100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 984 bp

ATGGAGTGGACTTGGCCGGAGAGTGTGTCGTCGAACCGGCAGAGGGTCGTGGAGGAGTTGGTTCAAGGCCGGGAGATGGTGAATCAGCTTCGGAGGTGTCTCAGTGGTGACGTCAGATCGGCGGAGGGTCTTGTTACGAAGATCTTGGGGTCGTTCACAAACACCCTTCGCATCTTGAATGGGAGTGAAGAGGCTAAAGAGGAGCTCATCTCTCAGATTCAAGCCAATAGTCCTACTAGTACTGTTACTGCTTCTGGTTCTGGTTCTGCTGCTGCTGGTACCGATTCATTGTCTTGGGATGCTCTTGATGATGCTGTTAAGTCCGAGGATTCGAATGAAGAGAGTTACAAGAGTACCGAAAAGTTCAAGGATCGGAGAGGTTCATACAAGAGAAGAAAAACTTCACATTCATGGACCAAAGACACTCTGGATTTAGTGGACGATGGCCATGCATGGAGGAAGTACGGACAAAAGATGATCCTGAATACCAGACATCCAAGGAACTACTTCAGGTGCACTCATAAGTTTGATCAAGGCTGCAAAGCGACCAAACATGTGCAGAGGATTAAAGATGAACCCCAACTCTTTCGGACCACATACTATGGCAATCACACATGCACTGACTATCTCAGAGCTCCTGAGCTCATCTTAGATTCTTCAAGTTCTCAAGACTCTTCCAAGAATATGATCAGATTCGACAACACCAACAACGACTTCACCCGCAAACAAGAACACCCCTTTTTCTCATCCTTCACATTGATCGAAAAAGAGAGCGTCAAGGAAGAAGAGATGATCACACCAATTAATGATCATGATCATGTGATTCACCACAACCAACAATTGTCGTCGTGTGACTATCTCATGTCGCCTGGTCTAGGAGTGTTTGAGTCGTCCGCATCGACTATCGCATATGATCACGAGGATGTGATTTCTAGGATAATGGTGGGATACTTCGATGATGAAGTAGATTTAATTTCCATATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0002218 GO:0002252 GO:0002253 GO:0002376 GO:0002682 GO:0002684 GO:0003674 GO:0003700 GO:0006355 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009627 GO:0009682 GO:0009719 GO:0009725 GO:0009751 GO:0009753 GO:0009755 GO:0009814 GO:0009862 GO:0009863 GO:0009864 GO:0009867 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010200 GO:0010243 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0014070 GO:0019219 GO:0019222 GO:0023052 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031347 GO:0031349 GO:0032870 GO:0035690 GO:0042221 GO:0042493 GO:0042742 GO:0043207 GO:0045087 GO:0045088 GO:0045089 GO:0045892 GO:0045934 GO:0046677 GO:0048518 GO:0048519 GO:0048523 GO:0048583 GO:0048584 GO:0048831 GO:0050776 GO:0050778 GO:0050789 GO:0050793 GO:0050794 GO:0050832 GO:0050896 GO:0051093 GO:0051171 GO:0051172 GO:0051239 GO:0051241 GO:0051252 GO:0051253 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0065007 GO:0070887 GO:0071229 GO:0071236 GO:0071310 GO:0071395 GO:0071407 GO:0071446 GO:0071495 GO:0080090 GO:0080134 GO:0098542 GO:0140110 GO:1900055 GO:1900056 GO:1901698 GO:1901700 GO:1901701 GO:1902679 GO:1903506 GO:1903507 GO:1905622 GO:2000024 GO:2000026 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WRKY

Protein Analysis

327

Amino Acids

37.34

Weight (kDa)

5.58

Isoelectric Point (pI)

53.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WRKY	PF03106	147 - 206	1.6e-24	WRKY DNA -binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001288)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G22570
fragaria_vesca	FvH4_6g09650 FvH4_6g09710 FvH4_6g09710 FvH4_6g09730 FvH4_6g09740 FvH4_6g09740 FvH4_6g09740 FvH4_6g09740 FvH4_6g09740 FvH4_6g09740 FvH4_6g09750 FvH4_6g09760
malus_domestica	MD04G1175500.v1.1 MD04G1175600.v1.1 MD12G1189200.v1.1 MD12G1189400.v1.1 MD12G1189600.v1.1 MD12G1189700.v1.1 MD12G1189900.v1.1
prunus_persica	Prupe.6G294900_v2.0.a1 Prupe.6G295000_v2.0.a1 Prupe.6G295100_v2.0.a1
pyrus_communis	pycom04g15480 pycom04g15490 pycom12g17650 pycom12g17690 pycom12g17700 pycom12g17730
rosa_chinensis	RchiOBHm_Chr3g0460351 RchiOBHm_Chr3g0460361
rosa_laevigata	RLG00000025018 RLG00000025019 RLG00000025027 RLG00000025113
rosa_multiflora	Rmu_sc0000449.1_g000005 Rmu_sc0004267.1_g000019
rosa_roxburghii	Rroxscaffold_6G00419560 Rroxscaffold_6G00419590
rosa_rugosa	Rorug03G0033500 Rorug03G0033500 Rorug03G0041400 Rorug03G0041800 Rorug03G0042000
rosa_samantha	Rh3AG100000 Rh3AG100100 Rh3BG094800 Rh3BG103900 Rh3BG104000 Rh3CG105700 Rh3CG105800 Rh3DG105300 Rh3DG105400
rosa_wichuraiana	Rw3G008480 Rw3G008490

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	114
Acc65I	GGTACC	1 cut(s)	278
AccB1I	GGYRCC	1 cut(s)	278
AciI	CCGC	3 cut(s)	122, 721, 894
AclWI	GGATC	2 cut(s)	378, 472
AcoI	YGGCCR	2 cut(s)	14, 445
AcuI	CTGAAG	1 cut(s)	493
AcyI	GRCGYC	1 cut(s)	111
AfaI	GTAC	4 cut(s)	241, 280, 355, 464
AfiI	CCNNNNNNNGG	3 cut(s)	43, 76, 875
AflIII	ACRYGT	1 cut(s)	553
AgsI	TTSAA	5 cut(s)	65, 178, 221, 367, 660
AhlI	ACTAGT	1 cut(s)	236
AjnI	CCWGG	1 cut(s)	868
AjuI	GAANNNNNNNTTGG	2 cut(s)	698, 730
AluBI	AGCT	4 cut(s)	88, 205, 635, 643
AluI	AGCT	4 cut(s)	88, 205, 635, 643
Alw21I	GWGCWC	4 cut(s)	207, 518, 637, 645
Alw26I	GTCTC	1 cut(s)	104
Alw44I	GTGCAC	1 cut(s)	514
AlwI	GGATC	2 cut(s)	378, 472
AoxI	GGCC	3 cut(s)	14, 67, 445
ApaLI	GTGCAC	1 cut(s)	514
ApeKI	GCWGC	3 cut(s)	269, 272, 537
AseI	ATTAAT	1 cut(s)	804
Asp700I	GAANNNNTTC	2 cut(s)	400, 506
Asp718I	GGTACC	1 cut(s)	278
AspS9I	GGNCC	2 cut(s)	414, 591
AsuC2I	CCSGG	1 cut(s)	71
AsuHPI	GGTGA	4 cut(s)	91, 119, 709, 818
AsuII	TTCGAA	1 cut(s)	332
AvaII	GGWCC	2 cut(s)	414, 591
BaeGI	GKGCMC	1 cut(s)	518
BaeI	ACNNNNGTAYC	2 cut(s)	337, 370
BalI	TGGCCA	1 cut(s)	447
BanI	GGYRCC	1 cut(s)	278
BanII	GRGCYC	3 cut(s)	207, 637, 645
BauI	CACGAG	1 cut(s)	917
Bbv12I	GWGCWC	4 cut(s)	207, 518, 637, 645
BbvI	GCAGC	3 cut(s)	256, 259, 524
BccI	CCATC	2 cut(s)	70, 437
BcgI	CGANNNNNNTGC	2 cut(s)	431, 465
BciT130I	CCWGG	1 cut(s)	870
BciVI	GTATCC	1 cut(s)	941
BclI	TGATCA	6 cut(s)	529, 687, 792, 808, 814, 913
BcnI	CCSGG	1 cut(s)	71
BcoDI	GTCTC	1 cut(s)	104
BcuI	ACTAGT	1 cut(s)	236
BfaI	CTAG	3 cut(s)	237, 875, 933
BfuI	GTATCC	1 cut(s)	941
BglII	AGATCT	1 cut(s)	141
BisI	GCNGC	3 cut(s)	270, 273, 538
BlsI	GCNGC	3 cut(s)	271, 274, 539
BmcAI	AGTACT	1 cut(s)	241
Bme1390I	CCNGG	2 cut(s)	71, 870
Bme18I	GGWCC	2 cut(s)	414, 591
BmgT120I	GGNCC	2 cut(s)	414, 591
BmiI	GGNNCC	1 cut(s)	280
BmrFI	CCNGG	2 cut(s)	71, 870
BmsI	GCATC	4 cut(s)	180, 289, 301, 905
BplI	GAGNNNNNCTC	2 cut(s)	194, 226
Bpu10I	CCTNAGC	1 cut(s)	639
Bpu14I	TTCGAA	1 cut(s)	332
BpuEI	CTTGAG	1 cut(s)	651
BpuMI	CCSGG	1 cut(s)	71
BsaBI	GATNNNNATC	1 cut(s)	813
BsaHI	GRCGYC	1 cut(s)	111
BsaJI	CCNNGG	2 cut(s)	324, 497
BsaXI	ACNNNNNCTCC	2 cut(s)	11, 41
Bsc4I	CCNNNNNNNGG	3 cut(s)	43, 76, 875
Bse118I	RCCGGY	1 cut(s)	36
Bse8I	GATNNNNATC	1 cut(s)	813
BseBI	CCWGG	1 cut(s)	870
BseDI	CCNNGG	2 cut(s)	324, 497
BseGI	GGATG	4 cut(s)	304, 493, 746, 928
BseJI	GATNNNNATC	1 cut(s)	813
BseLI	CCNNNNNNNGG	3 cut(s)	43, 76, 875
BseMII	CTCAG	4 cut(s)	115, 227, 630, 643
BseRI	GAGGAG	2 cut(s)	68, 215
BseSI	GKGCMC	1 cut(s)	518
BseXI	GCAGC	3 cut(s)	256, 259, 524
BsgI	GTGCAG	1 cut(s)	578
BshFI	GGCC	3 cut(s)	16, 69, 447
BshNI	GGYRCC	1 cut(s)	278
BsiHKAI	GWGCWC	4 cut(s)	207, 518, 637, 645
BsiSI	CCGG	3 cut(s)	17, 37, 70
BslI	CCNNNNNNNGG	3 cut(s)	43, 76, 875
BsmAI	GTCTC	1 cut(s)	104
BsnI	GGCC	3 cut(s)	16, 69, 447
Bsp119I	TTCGAA	1 cut(s)	332
Bsp1286I	GDGCHC	4 cut(s)	207, 518, 637, 645
BspACI	CCGC	3 cut(s)	122, 721, 894
BspANI	GGCC	3 cut(s)	16, 69, 447
BspCNI	CTCAG	4 cut(s)	114, 226, 631, 642
BspHI	TCATGA	1 cut(s)	811
BspLI	GGNNCC	1 cut(s)	280
BspPI	GGATC	2 cut(s)	378, 472
BspT104I	TTCGAA	1 cut(s)	332
BspT107I	GGYRCC	1 cut(s)	278
BsrFI	RCCGGY	1 cut(s)	36
BssAI	RCCGGY	1 cut(s)	36
BssECI	CCNNGG	2 cut(s)	324, 497
BssNI	GRCGYC	1 cut(s)	111
BssSI	CACGAG	1 cut(s)	917
BssT1I	CCWWGG	1 cut(s)	497
Bst2BI	CACGAG	1 cut(s)	917
Bst2UI	CCWGG	1 cut(s)	870
Bst4CI	ACNGT	1 cut(s)	244
Bst6I	CTCTTC	4 cut(s)	183, 333, 679, 780
BstACI	GRCGYC	1 cut(s)	111
BstBI	TTCGAA	1 cut(s)	332
BstDEI	CTNAG	5 cut(s)	101, 213, 629, 639, 649
BstENI	CCTNNNNNAGG	1 cut(s)	873
BstF5I	GGATG	4 cut(s)	304, 493, 746, 928
BstMAI	GTCTC	1 cut(s)	104
BstNI	CCWGG	1 cut(s)	870
BstNSI	RCATGY	2 cut(s)	557, 618
BstSCI	CCNGG	2 cut(s)	69, 868
BstSLI	GKGCMC	1 cut(s)	518
BstV1I	GCAGC	3 cut(s)	256, 259, 524
BstX2I	RGATCY	1 cut(s)	141
BstYI	RGATCY	1 cut(s)	141
BsuI	GTATCC	1 cut(s)	941
BsuRI	GGCC	3 cut(s)	16, 69, 447
BtsCI	GGATG	4 cut(s)	304, 493, 746, 928
BtsIMutI	CAGTG	2 cut(s)	109, 618
CciI	TCATGA	1 cut(s)	811
Cfr10I	RCCGGY	1 cut(s)	36
Cfr13I	GGNCC	2 cut(s)	414, 591
CseI	GACGC	1 cut(s)	763
Csp6I	GTAC	4 cut(s)	240, 279, 354, 463
CviAII	CATG	8 cut(s)	411, 449, 453, 554, 615, 812, 818, 862
CviQI	GTAC	4 cut(s)	240, 279, 354, 463
DdeI	CTNAG	5 cut(s)	101, 213, 629, 639, 649
EaeI	YGGCCR	2 cut(s)	14, 445
Eam1104I	CTCTTC	4 cut(s)	183, 333, 679, 780
EarI	CTCTTC	4 cut(s)	183, 333, 679, 780
EciI	GGCGGA	1 cut(s)	137
Ecl136II	GAGCTC	3 cut(s)	205, 635, 643
Eco130I	CCWWGG	1 cut(s)	497
Eco24I	GRGCYC	3 cut(s)	207, 637, 645
Eco47I	GGWCC	2 cut(s)	414, 591
Eco53kI	GAGCTC	3 cut(s)	205, 635, 643
Eco57I	CTGAAG	1 cut(s)	493
EcoICRI	GAGCTC	3 cut(s)	205, 635, 643
EcoNI	CCTNNNNNAGG	1 cut(s)	873
EcoRII	CCWGG	1 cut(s)	868
EcoT14I	CCWWGG	1 cut(s)	497
EcoT22I	ATGCAT	1 cut(s)	454
EcoT38I	GRGCYC	3 cut(s)	207, 637, 645
ErhI	CCWWGG	1 cut(s)	497
FaeI	CATG	8 cut(s)	414, 452, 456, 557, 618, 815, 821, 865
FalI	AAGNNNNNCTT	2 cut(s)	385, 417
FatI	CATG	8 cut(s)	410, 448, 452, 553, 614, 811, 817, 861
FauI	CCCGC	1 cut(s)	728
FauNDI	CATATG	2 cut(s)	910, 980
FbaI	TGATCA	6 cut(s)	529, 687, 792, 808, 814, 913
Fnu4HI	GCNGC	3 cut(s)	270, 273, 538
FokI	GGATG	4 cut(s)	311, 480, 733, 935
FriOI	GRGCYC	3 cut(s)	207, 637, 645
Fsp4HI	GCNGC	3 cut(s)	270, 273, 538
FspBI	CTAG	3 cut(s)	237, 875, 933
GluI	GCNGC	3 cut(s)	270, 273, 538
HaeIII	GGCC	3 cut(s)	16, 69, 447
HapII	CCGG	3 cut(s)	17, 37, 70
HgaI	GACGC	1 cut(s)	763
Hin1I	GRCGYC	1 cut(s)	111
Hin1II	CATG	8 cut(s)	414, 452, 456, 557, 618, 815, 821, 865
HinfI	GANTC	9 cut(s)	82, 217, 284, 329, 653, 671, 693, 823, 887
HpaII	CCGG	3 cut(s)	17, 37, 70
HphI	GGTGA	4 cut(s)	91, 119, 709, 818
Hpy166II	GTNNAC	4 cut(s)	9, 156, 439, 516
Hpy188I	TCNGA	8 cut(s)	93, 116, 216, 325, 375, 591, 632, 692
Hpy188III	TCNNGA	8 cut(s)	175, 305, 428, 481, 638, 668, 812, 917
Hpy8I	GTNNAC	4 cut(s)	9, 156, 439, 516
Hpy99I	CGWCG	2 cut(s)	34, 850
HpyAV	CCTTC	2 cut(s)	176, 760
HpyCH4III	ACNGT	1 cut(s)	244
HpyCH4IV	ACGT	1 cut(s)	111
HpyCH4V	TGCA	5 cut(s)	452, 516, 540, 559, 618
HpyF3I	CTNAG	5 cut(s)	101, 213, 629, 639, 649
HpySE526I	ACGT	1 cut(s)	111
Hsp92I	GRCGYC	1 cut(s)	111
Hsp92II	CATG	8 cut(s)	414, 452, 456, 557, 618, 815, 821, 865
KpnI	GGTACC	1 cut(s)	282
Ksp22I	TGATCA	6 cut(s)	529, 687, 792, 808, 814, 913
LmnI	GCTCC	2 cut(s)	202, 640
Lsp1109I	GCAGC	3 cut(s)	256, 259, 524
LweI	GCATC	4 cut(s)	180, 289, 301, 905
MaeI	CTAG	3 cut(s)	237, 875, 933
MaeII	ACGT	1 cut(s)	111
MaeIII	GTNAC	5 cut(s)	107, 133, 244, 344, 851
MboII	GAAGA	8 cut(s)	151, 200, 350, 405, 648, 666, 794, 797
MfeI	CAATTG	1 cut(s)	839
MflI	RGATCY	1 cut(s)	141
MhlI	GDGCHC	4 cut(s)	207, 518, 637, 645
MlsI	TGGCCA	1 cut(s)	447
MluCI	AATT	3 cut(s)	801, 839, 972
MluNI	TGGCCA	1 cut(s)	447
MlyI	GAGTC	2 cut(s)	665, 896
Mox20I	TGGCCA	1 cut(s)	447
Mph1103I	ATGCAT	1 cut(s)	454
MroXI	GAANNNNTTC	2 cut(s)	400, 506
MscI	TGGCCA	1 cut(s)	447
MseI	TTAA	4 cut(s)	318, 567, 804, 971
MslI	CAYNNNNRTG	3 cut(s)	409, 816, 921
Msp20I	TGGCCA	1 cut(s)	447
MspI	CCGG	3 cut(s)	17, 37, 70
MspR9I	CCNGG	2 cut(s)	71, 870
MunI	CAATTG	1 cut(s)	839
MvaI	CCWGG	1 cut(s)	870
NciI	CCSGG	1 cut(s)	71
NdeI	CATATG	2 cut(s)	910, 980
NlaIII	CATG	8 cut(s)	414, 452, 456, 557, 618, 815, 821, 865
NlaIV	GGNNCC	1 cut(s)	280
NmuCI	GTSAC	2 cut(s)	107, 851
NsiI	ATGCAT	1 cut(s)	454
NspI	RCATGY	2 cut(s)	557, 618
NspV	TTCGAA	1 cut(s)	332
PagI	TCATGA	1 cut(s)	811
PciI	ACATGT	1 cut(s)	553
PcsI	WCGNNNNNNNCGW	1 cut(s)	896
PdmI	GAANNNNTTC	2 cut(s)	400, 506
PfeI	GAWTC	7 cut(s)	82, 217, 284, 329, 653, 693, 823
PkrI	GCNGC	3 cut(s)	271, 274, 539
PleI	GAGTC	2 cut(s)	665, 895
PpsI	GAGTC	2 cut(s)	665, 895
PscI	ACATGT	1 cut(s)	553
PshBI	ATTAAT	1 cut(s)	804
Psp124BI	GAGCTC	3 cut(s)	207, 637, 645
Psp6I	CCWGG	1 cut(s)	868
PspGI	CCWGG	1 cut(s)	868
PspN4I	GGNNCC	1 cut(s)	280
PspPI	GGNCC	2 cut(s)	414, 591
PsuI	RGATCY	1 cut(s)	141
RsaI	GTAC	4 cut(s)	241, 280, 355, 464
RsaNI	GTAC	4 cut(s)	240, 279, 354, 463
RseI	CAYNNNNRTG	3 cut(s)	409, 816, 921
SacI	GAGCTC	3 cut(s)	207, 637, 645
SaqAI	TTAA	4 cut(s)	318, 567, 804, 971
SatI	GCNGC	3 cut(s)	270, 273, 538
Sau96I	GGNCC	2 cut(s)	414, 591
ScaI	AGTACT	1 cut(s)	241
SchI	GAGTC	2 cut(s)	665, 896
ScrFI	CCNGG	2 cut(s)	71, 870
SduI	GDGCHC	4 cut(s)	207, 518, 637, 645
SetI	ASST	8 cut(s)	90, 98, 114, 207, 382, 515, 637, 645
SfaNI	GCATC	4 cut(s)	180, 289, 301, 905
SfuI	TTCGAA	1 cut(s)	332
SinI	GGWCC	2 cut(s)	414, 591
SmiMI	CAYNNNNRTG	3 cut(s)	409, 816, 921
SmlI	CTYRAG	1 cut(s)	666
SmoI	CTYRAG	1 cut(s)	666
SpeI	ACTAGT	1 cut(s)	236
Sse9I	AATT	3 cut(s)	801, 839, 972
SsiI	CCGC	3 cut(s)	122, 721, 894
SspMI	CTAG	3 cut(s)	237, 875, 933
SstI	GAGCTC	3 cut(s)	207, 637, 645
StyD4I	CCNGG	2 cut(s)	69, 868
StyI	CCWWGG	1 cut(s)	497
TaaI	ACNGT	1 cut(s)	244
TaiI	ACGT	1 cut(s)	114
TaqI	TCGA	6 cut(s)	32, 332, 696, 762, 899, 954
TasI	AATT	3 cut(s)	801, 839, 972
TatI	WGTACW	1 cut(s)	239
TfiI	GAWTC	7 cut(s)	82, 217, 284, 329, 653, 693, 823
Tru1I	TTAA	4 cut(s)	318, 567, 804, 971
Tru9I	TTAA	4 cut(s)	318, 567, 804, 971
TscAI	CASTG	2 cut(s)	109, 625
TseFI	GTSAC	2 cut(s)	107, 851
TseI	GCWGC	3 cut(s)	269, 272, 537
Tsp45I	GTSAC	2 cut(s)	107, 851
TspDTI	ATGAA	6 cut(s)	276, 351, 372, 399, 588, 975
TspGWI	ACGGA	1 cut(s)	480
TspRI	CASTG	2 cut(s)	109, 625
VneI	GTGCAC	1 cut(s)	514
VpaK11BI	GGWCC	2 cut(s)	414, 591
VspI	ATTAAT	1 cut(s)	804
XagI	CCTNNNNNAGG	1 cut(s)	873
XceI	RCATGY	2 cut(s)	557, 618
XcmI	CCANNNNNNNNNTGG	1 cut(s)	424
XmnI	GAANNNNTTC	2 cut(s)	400, 506
XspI	CTAG	3 cut(s)	237, 875, 933
ZraI	GACGTC	1 cut(s)	112
ZrmI	AGTACT	1 cut(s)	241
Zsp2I	ATGCAT	1 cut(s)	454

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.