Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0001288

Physical Location & Seq

Genomic Coordinates Reverse (-)

27190989 .. 27194447

Gene Structure 3459 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1189600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1110 bp

ATGGAGTGGTCGTGGCCGGAAACTGTGCTCTCAAATAGGGAGAGGGTGATGGAGGAGTTGATCCAAGGGCGTGAACTGGCAAGTCAACTTAGTAAGGCTTTTGATAATAGGTCACTTGTTAATGGTGATGATTGGGGATCGGCTGAAGGTATTGTTAATAAGATTTTGGGGTCATTCGTTAGTACCCTCTTGATTATAAATGGGAAGGAGTCTGTTCAGGAGTTTGTTCAGGAGTTTGTTTCTGATCAGATTCAAGGAAATAGTAGTGGTGTGAGTGGCGATGGTAGTGGTGGTGGTAGTGGTGTTGGTGGCGGTGGTAGTGGTGTTGGTAGTGGTGTTGGTGACGGTGGTAGTGATGGTGGTGGTGGTGCTTATTCATCATCTGCGGATGCTAATCATGATCATGCAGCTGCTATGAAGTCTGAAGATTGTACTGAGGAGGAGATCAGTTGTAAGAGCACGTTAACCTTCAAGGATGGTAGAGGTTCTTACAAGAGAAGAAAGACTTCACACTCTTGGACTAGAGATACTCCTGCGTTGATTGCCGACGGTCATGCATGGAGAAAGTATGGACAAAAAATTATCCACAATGCCAAGCATCCAAGGAACTACTTTAGATGCACACACAAATACGATCAAGCGTGCAAAGCAACCAAACACGTGCAACAAGTTCAAGATCATTCACCAGTGTTTCGAACCATATATTATGGCACCCACAAGTGCAGAGACCACCTCAAAACTTCTAAACTGCTCTTGGATTGTACAAGTCCTAGAGATTCTTCACAGTTCATTAGTTTTGAAAACGTCAATTGTATGACAAACAAACAAGAGCACCCATTTTTCGCATCCTTCGCTTCATCATCGGTTAAAGAAGAAGTGTTCGTCAAAGAAGATATGCCAACAACTGATCATATGGTGACAAGCGACCACAACCATTCATCACCGTGCGATCATCTTGTGTCGCGTGATCTGACGGCGTTCGAGTCCTCTGGGCCCATGAGCGGGTTTTCATCAAGCATTGATCAGTATGATCATGACAATATGTTTTGGGGGATGATTGCCGAGTCTTACTTTGATAAATATAATGAAGTTTTGCAATGTGGATTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0002218 GO:0002252 GO:0002253 GO:0002376 GO:0002682 GO:0002684 GO:0003674 GO:0003700 GO:0006355 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009627 GO:0009682 GO:0009719 GO:0009725 GO:0009751 GO:0009753 GO:0009755 GO:0009814 GO:0009862 GO:0009863 GO:0009864 GO:0009867 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010200 GO:0010243 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0014070 GO:0019219 GO:0019222 GO:0023052 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031347 GO:0031349 GO:0032870 GO:0035690 GO:0042221 GO:0042493 GO:0042742 GO:0043207 GO:0045087 GO:0045088 GO:0045089 GO:0045892 GO:0045934 GO:0046677 GO:0048518 GO:0048519 GO:0048523 GO:0048583 GO:0048584 GO:0048831 GO:0050776 GO:0050778 GO:0050789 GO:0050793 GO:0050794 GO:0050832 GO:0050896 GO:0051093 GO:0051171 GO:0051172 GO:0051239 GO:0051241 GO:0051252 GO:0051253 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0065007 GO:0070887 GO:0071229 GO:0071236 GO:0071310 GO:0071395 GO:0071407 GO:0071446 GO:0071495 GO:0080090 GO:0080134 GO:0098542 GO:0140110 GO:1900055 GO:1900056 GO:1901698 GO:1901700 GO:1901701 GO:1902679 GO:1903506 GO:1903507 GO:1905622 GO:2000024 GO:2000026 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WRKY

Protein Analysis

370

Amino Acids

40.6

Weight (kDa)

5.69

Isoelectric Point (pI)

47.22

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WRKY	PF03106	183 - 241	4e-21	WRKY DNA -binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001288)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G22570
fragaria_vesca	FvH4_6g09650 FvH4_6g09710 FvH4_6g09710 FvH4_6g09730 FvH4_6g09740 FvH4_6g09740 FvH4_6g09740 FvH4_6g09740 FvH4_6g09740 FvH4_6g09740 FvH4_6g09750 FvH4_6g09760
malus_domestica	MD04G1175500.v1.1 MD04G1175600.v1.1 MD12G1189200.v1.1 MD12G1189400.v1.1 MD12G1189600.v1.1 MD12G1189700.v1.1 MD12G1189900.v1.1
prunus_persica	Prupe.6G294900_v2.0.a1 Prupe.6G295000_v2.0.a1 Prupe.6G295100_v2.0.a1
pyrus_communis	pycom04g15480 pycom04g15490 pycom12g17650 pycom12g17690 pycom12g17700 pycom12g17730
rosa_chinensis	RchiOBHm_Chr3g0460351 RchiOBHm_Chr3g0460361
rosa_laevigata	RLG00000025018 RLG00000025019 RLG00000025027 RLG00000025113
rosa_multiflora	Rmu_sc0000449.1_g000005 Rmu_sc0004267.1_g000019
rosa_roxburghii	Rroxscaffold_6G00419560 Rroxscaffold_6G00419590
rosa_rugosa	Rorug03G0033500 Rorug03G0033500 Rorug03G0041400 Rorug03G0041800 Rorug03G0042000
rosa_samantha	Rh3AG100000 Rh3AG100100 Rh3BG094800 Rh3BG103900 Rh3BG104000 Rh3CG105700 Rh3CG105800 Rh3DG105300 Rh3DG105400
rosa_wichuraiana	Rw3G008480 Rw3G008490

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	197
AccB1I	GGYRCC	1 cut(s)	710
AccBSI	CCGCTC	1 cut(s)	1002
AccII	CGCG	1 cut(s)	964
AciI	CCGC	3 cut(s)	312, 386, 1002
AclWI	GGATC	2 cut(s)	55, 145
AcoI	YGGCCR	1 cut(s)	14
AcuI	CTGAAG	2 cut(s)	165, 444
AcvI	CACGTG	1 cut(s)	661
AfaI	GTAC	3 cut(s)	184, 433, 763
AfiI	CCNNNNNNNGG	2 cut(s)	1001, 1002
AflIII	ACRYGT	1 cut(s)	658
AgsI	TTSAA	4 cut(s)	254, 472, 674, 800
AluBI	AGCT	1 cut(s)	410
AluI	AGCT	1 cut(s)	410
Alw21I	GWGCWC	3 cut(s)	30, 461, 834
Alw26I	GTCTC	1 cut(s)	720
AlwI	GGATC	2 cut(s)	55, 145
AoxI	GGCC	2 cut(s)	14, 992
ApaI	GGGCCC	1 cut(s)	996
ApeKI	GCWGC	2 cut(s)	407, 410
Asp700I	GAANNNNTTC	1 cut(s)	505
AspS9I	GGNCC	2 cut(s)	992, 993
AsuHPI	GGTGA	6 cut(s)	58, 137, 353, 675, 928, 933
AsuII	TTCGAA	1 cut(s)	694
BaeGI	GKGCMC	1 cut(s)	996
BanI	GGYRCC	1 cut(s)	710
BanII	GRGCYC	1 cut(s)	996
BbrPI	CACGTG	1 cut(s)	661
Bbv12I	GWGCWC	3 cut(s)	30, 461, 834
BbvI	GCAGC	2 cut(s)	397, 419
BccI	CCATC	4 cut(s)	43, 275, 350, 470
BceAI	ACGGC	1 cut(s)	990
BcgI	CGANNNNNNTGC	2 cut(s)	536, 570
BclI	TGATCA	5 cut(s)	244, 400, 907, 1021, 1030
BcoDI	GTCTC	1 cut(s)	720
BfaI	CTAG	2 cut(s)	522, 771
BisI	GCNGC	2 cut(s)	408, 411
BlsI	GCNGC	2 cut(s)	409, 412
BmgT120I	GGNCC	2 cut(s)	992, 993
BmiI	GGNNCC	2 cut(s)	712, 994
BmsI	GCATC	4 cut(s)	379, 607, 608, 854
BplI	GAGNNNNNCTC	2 cut(s)	717, 749
Bpu14I	TTCGAA	1 cut(s)	694
BsaAI	YACGTR	1 cut(s)	661
BsaBI	GATNNNNATC	1 cut(s)	393
BsaI	GGTCTC	1 cut(s)	720
BsaJI	CCNNGG	2 cut(s)	64, 602
BsaXI	ACNNNNNCTCC	1 cut(s)	26
Bsc4I	CCNNNNNNNGG	2 cut(s)	1001, 1002
Bse1I	ACTGG	2 cut(s)	81, 686
Bse3DI	GCAATG	1 cut(s)	1103
Bse8I	GATNNNNATC	1 cut(s)	393
BseDI	CCNNGG	2 cut(s)	64, 602
BseGI	GGATG	5 cut(s)	394, 481, 598, 845, 1059
BseJI	GATNNNNATC	1 cut(s)	393
BseLI	CCNNNNNNNGG	2 cut(s)	1001, 1002
BseMI	GCAATG	1 cut(s)	1103
BseMII	CTCAG	1 cut(s)	426
BseNI	ACTGG	2 cut(s)	81, 686
BseRI	GAGGAG	3 cut(s)	68, 452, 455
BseSI	GKGCMC	1 cut(s)	996
BseXI	GCAGC	2 cut(s)	397, 419
BsgI	GTGCAG	1 cut(s)	742
Bsh1236I	CGCG	1 cut(s)	964
BshFI	GGCC	2 cut(s)	16, 994
BshNI	GGYRCC	1 cut(s)	710
BsiHKAI	GWGCWC	3 cut(s)	30, 461, 834
BsiSI	CCGG	1 cut(s)	17
BslI	CCNNNNNNNGG	2 cut(s)	1001, 1002
BsmAI	GTCTC	1 cut(s)	720
BsnI	GGCC	2 cut(s)	16, 994
Bso31I	GGTCTC	1 cut(s)	720
Bsp119I	TTCGAA	1 cut(s)	694
Bsp120I	GGGCCC	1 cut(s)	992
Bsp1286I	GDGCHC	4 cut(s)	30, 461, 834, 996
Bsp1407I	TGTACA	1 cut(s)	761
BspACI	CCGC	3 cut(s)	312, 386, 1002
BspANI	GGCC	2 cut(s)	16, 994
BspCNI	CTCAG	1 cut(s)	427
BspFNI	CGCG	1 cut(s)	964
BspHI	TCATGA	2 cut(s)	397, 1033
BspLI	GGNNCC	2 cut(s)	712, 994
BspPI	GGATC	2 cut(s)	55, 145
BspT104I	TTCGAA	1 cut(s)	694
BspT107I	GGYRCC	1 cut(s)	710
BspTNI	GGTCTC	1 cut(s)	720
BsrBI	CCGCTC	1 cut(s)	1002
BsrDI	GCAATG	1 cut(s)	1103
BsrGI	TGTACA	1 cut(s)	761
BsrI	ACTGG	2 cut(s)	81, 686
BssECI	CCNNGG	2 cut(s)	64, 602
BssT1I	CCWWGG	2 cut(s)	64, 602
Bst4CI	ACNGT	5 cut(s)	25, 347, 551, 786, 945
BstAUI	TGTACA	1 cut(s)	761
BstBAI	YACGTR	1 cut(s)	661
BstBI	TTCGAA	1 cut(s)	694
BstC8I	GCNNGC	1 cut(s)	643
BstDEI	CTNAG	2 cut(s)	89, 435
BstF5I	GGATG	5 cut(s)	394, 481, 598, 845, 1059
BstFNI	CGCG	1 cut(s)	964
BstMAI	GTCTC	1 cut(s)	720
BstMWI	GCNNNNNNNGC	3 cut(s)	542, 647, 851
BstSLI	GKGCMC	1 cut(s)	996
BstUI	CGCG	1 cut(s)	964
BstV1I	GCAGC	2 cut(s)	397, 419
BsuRI	GGCC	2 cut(s)	16, 994
BtgZI	GCGATG	1 cut(s)	294
BtsCI	GGATG	5 cut(s)	394, 481, 598, 845, 1059
BtsIMutI	CAGTG	1 cut(s)	693
Cac8I	GCNNGC	1 cut(s)	643
CciI	TCATGA	2 cut(s)	397, 1033
Cfr13I	GGNCC	2 cut(s)	992, 993
Csp6I	GTAC	3 cut(s)	183, 432, 762
CviAII	CATG	6 cut(s)	398, 404, 554, 558, 997, 1034
CviJI	RGCY	5 cut(s)	16, 98, 143, 410, 994
CviKI_1	RGCY	5 cut(s)	16, 98, 143, 410, 994
CviQI	GTAC	3 cut(s)	183, 432, 762
DdeI	CTNAG	2 cut(s)	89, 435
EaeI	YGGCCR	1 cut(s)	14
Eco130I	CCWWGG	2 cut(s)	64, 602
Eco24I	GRGCYC	1 cut(s)	996
Eco31I	GGTCTC	1 cut(s)	720
Eco57I	CTGAAG	2 cut(s)	165, 444
Eco72I	CACGTG	1 cut(s)	661
EcoT14I	CCWWGG	2 cut(s)	64, 602
EcoT22I	ATGCAT	1 cut(s)	559
EcoT38I	GRGCYC	1 cut(s)	996
ErhI	CCWWGG	2 cut(s)	64, 602
FaeI	CATG	6 cut(s)	401, 407, 557, 561, 1000, 1037
FalI	AAGNNNNNCTT	2 cut(s)	490, 522
FatI	CATG	6 cut(s)	397, 403, 553, 557, 996, 1033
FauI	CCCGC	1 cut(s)	995
FauNDI	CATATG	1 cut(s)	912
FbaI	TGATCA	5 cut(s)	244, 400, 907, 1021, 1030
Fnu4HI	GCNGC	2 cut(s)	408, 411
FokI	GGATG	5 cut(s)	401, 488, 585, 832, 1066
FriOI	GRGCYC	1 cut(s)	996
Fsp4HI	GCNGC	2 cut(s)	408, 411
FspBI	CTAG	2 cut(s)	522, 771
GluI	GCNGC	2 cut(s)	408, 411
HaeIII	GGCC	2 cut(s)	16, 994
HapII	CCGG	1 cut(s)	17
Hin1II	CATG	6 cut(s)	401, 407, 557, 561, 1000, 1037
HincII	GTYRAC	2 cut(s)	86, 465
HindII	GTYRAC	2 cut(s)	86, 465
HinfI	GANTC	5 cut(s)	209, 250, 776, 983, 1064
HpaI	GTTAAC	1 cut(s)	465
HpaII	CCGG	1 cut(s)	17
HphI	GGTGA	6 cut(s)	58, 137, 353, 675, 928, 933
Hpy166II	GTNNAC	3 cut(s)	74, 86, 465
Hpy188I	TCNGA	4 cut(s)	244, 249, 424, 972
Hpy188III	TCNNGA	6 cut(s)	190, 218, 230, 398, 674, 1034
Hpy8I	GTNNAC	3 cut(s)	74, 86, 465
Hpy99I	CGWCG	1 cut(s)	551
HpyAV	CCTTC	4 cut(s)	140, 199, 478, 859
HpyCH4III	ACNGT	5 cut(s)	25, 347, 551, 786, 945
HpyCH4IV	ACGT	3 cut(s)	461, 660, 804
HpyCH4V	TGCA	7 cut(s)	407, 557, 621, 645, 664, 723, 1096
HpyF10VI	GCNNNNNNNGC	3 cut(s)	542, 647, 851
HpyF3I	CTNAG	2 cut(s)	89, 435
HpySE526I	ACGT	3 cut(s)	461, 660, 804
Hsp92II	CATG	6 cut(s)	401, 407, 557, 561, 1000, 1037
Ksp22I	TGATCA	5 cut(s)	244, 400, 907, 1021, 1030
KspAI	GTTAAC	1 cut(s)	465
LpnPI	CCDG	7 cut(s)	30, 62, 203, 215, 546, 699, 975
Lsp1109I	GCAGC	2 cut(s)	397, 419
LweI	GCATC	4 cut(s)	379, 607, 608, 854
MaeI	CTAG	2 cut(s)	522, 771
MaeII	ACGT	3 cut(s)	461, 660, 804
MaeIII	GTNAC	3 cut(s)	111, 341, 916
MbiI	CCGCTC	1 cut(s)	1002
MboII	GAAGA	5 cut(s)	437, 510, 771, 884, 902
MfeI	CAATTG	1 cut(s)	808
MhlI	GDGCHC	4 cut(s)	30, 461, 834, 996
MluCI	AATT	2 cut(s)	579, 808
MlyI	GAGTC	3 cut(s)	218, 992, 1073
MnlI	CCTC	8 cut(s)	36, 46, 197, 430, 433, 476, 743, 997
Mph1103I	ATGCAT	1 cut(s)	559
MroXI	GAANNNNTTC	1 cut(s)	505
MseI	TTAA	4 cut(s)	120, 156, 464, 867
MslI	CAYNNNNRTG	2 cut(s)	402, 943
MspA1I	CMGCKG	1 cut(s)	410
MspI	CCGG	1 cut(s)	17
MunI	CAATTG	1 cut(s)	808
MvnI	CGCG	1 cut(s)	964
MwoI	GCNNNNNNNGC	3 cut(s)	542, 647, 851
NdeI	CATATG	1 cut(s)	912
NlaIII	CATG	6 cut(s)	401, 407, 557, 561, 1000, 1037
NlaIV	GGNNCC	2 cut(s)	712, 994
NmeAIII	GCCGAG	1 cut(s)	1087
NmuCI	GTSAC	3 cut(s)	111, 341, 916
NsiI	ATGCAT	1 cut(s)	559
NspV	TTCGAA	1 cut(s)	694
PagI	TCATGA	2 cut(s)	397, 1033
PdmI	GAANNNNTTC	1 cut(s)	505
PfeI	GAWTC	2 cut(s)	250, 776
PkrI	GCNGC	2 cut(s)	409, 412
PleI	GAGTC	3 cut(s)	217, 991, 1072
PmaCI	CACGTG	1 cut(s)	661
PmlI	CACGTG	1 cut(s)	661
PpsI	GAGTC	3 cut(s)	217, 991, 1072
Ppu21I	YACGTR	1 cut(s)	661
PsiI	TTATAA	1 cut(s)	197
PspCI	CACGTG	1 cut(s)	661
PspN4I	GGNNCC	2 cut(s)	712, 994
PspOMI	GGGCCC	1 cut(s)	992
PspPI	GGNCC	2 cut(s)	992, 993
PvuII	CAGCTG	1 cut(s)	410
RsaI	GTAC	3 cut(s)	184, 433, 763
RsaNI	GTAC	3 cut(s)	183, 432, 762
RseI	CAYNNNNRTG	2 cut(s)	402, 943
SaqAI	TTAA	4 cut(s)	120, 156, 464, 867
SatI	GCNGC	2 cut(s)	408, 411
Sau96I	GGNCC	2 cut(s)	992, 993
SchI	GAGTC	3 cut(s)	218, 992, 1073
SduI	GDGCHC	4 cut(s)	30, 461, 834, 996
SetI	ASST	9 cut(s)	113, 151, 412, 464, 470, 487, 663, 735, 807
SfaNI	GCATC	4 cut(s)	379, 607, 608, 854
SfuI	TTCGAA	1 cut(s)	694
SmiMI	CAYNNNNRTG	2 cut(s)	402, 943
Sse9I	AATT	2 cut(s)	579, 808
SsiI	CCGC	3 cut(s)	312, 386, 1002
SspMI	CTAG	2 cut(s)	522, 771
StyI	CCWWGG	2 cut(s)	64, 602
TaaI	ACNGT	5 cut(s)	25, 347, 551, 786, 945
TaiI	ACGT	3 cut(s)	464, 663, 807
TaqI	TCGA	2 cut(s)	694, 981
TasI	AATT	2 cut(s)	579, 808
TatI	WGTACW	2 cut(s)	431, 761
TfiI	GAWTC	2 cut(s)	250, 776
Tru1I	TTAA	4 cut(s)	120, 156, 464, 867
Tru9I	TTAA	4 cut(s)	120, 156, 464, 867
TscAI	CASTG	1 cut(s)	693
TseFI	GTSAC	3 cut(s)	111, 341, 916
TseI	GCWGC	2 cut(s)	407, 410
Tsp45I	GTSAC	3 cut(s)	111, 341, 916
TspDTI	ATGAA	7 cut(s)	366, 431, 778, 846, 927, 999, 1101
TspRI	CASTG	1 cut(s)	693
XmnI	GAANNNNTTC	1 cut(s)	505
XspI	CTAG	2 cut(s)	522, 771
Zsp2I	ATGCAT	1 cut(s)	559

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.