Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0001288

Physical Location & Seq

Genomic Coordinates Reverse (-)

27086797 .. 27089009

Gene Structure 2213 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1189200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1020 bp

ATGGAGTCGTCGTGGCCGGAGAGTCTGCTCTCAAATAGGGAGAGGGTGATGGAGGAGTTGATCCAAGGGCGTGAGCTGGCAAATCAACTTAGTAGGGCTTTTGATACTAGGTTACTTGTCGCTAATGGTCATGATGGGAGATCGGCTGAAGGTATTGTTAATAAGATTTTGGGGTCATTTGTGAATACCCTTTTGATTTTAAATGGGAAGGAGTCTGATCAAGAGTTTGTTTGCAATCAGATTCAAGGAAATAGTAGAGGTGTTGGTGGTGGTGGTAGTGGTGTTAGTGACGGTGGTAGTAGTGGTGGTGGTGGTGGTGCATATTCGTCATCTTGGGGTGCTAATCATGGTCATGCAGCTGCTATTAAGCCTGAAGACTATACTGAGGAGAACAGTTGTAGAAGCACTTCAACCTTCAAGGATCGAAGAGGTTCTTACAAGAGAAGAAAGACTTCACACTCTGGGACTAGAGACACTCCTGCTTTGATTGATGACGGTCATGCATGGAGAAAGTATGGACAAAAAAATATCCACAATGCCAAGCATCCAAGGAACTACTTTAGGTGCACTCACAAACACGATCAATCGTGCAAAGCAACCAAGCACGTTCAACAAGTTCAAGATCATCCACCAGTGGTTCGAACCACATATTATGGCACCCACACGTGCAGAGACTACCTCAAAACTTCTGAACTGGTCTTGGATTGCACAAGTCCTAGAGATTCTTCAAAGTTCATTAGTTTTGAGAACGCCAACTGGTTGACAAACAAACAAGAGCATCCCTTTTTCGCAACCTTCGCTTCATCATCAGTTAAAGAAGAAGTGTTCGTTAAAGAAGATATTGCAACAACTGATCACATAGTGACAAGCGACCACAACCATTCCGTACGGCGTGATCATCTTGTGTCGCATGATCTGACGGCGTTCGAGTCCTCTGGGCCCATGGGTGGGTTTTCATCAAACATCGATCAGTATGAAGATGACGACATGTTTTGGAAGACTATCGAGTCTTTACTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0002218 GO:0002252 GO:0002253 GO:0002376 GO:0002682 GO:0002684 GO:0003674 GO:0003700 GO:0006355 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009627 GO:0009682 GO:0009719 GO:0009725 GO:0009751 GO:0009753 GO:0009755 GO:0009814 GO:0009862 GO:0009863 GO:0009864 GO:0009867 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010200 GO:0010243 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0014070 GO:0019219 GO:0019222 GO:0023052 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031347 GO:0031349 GO:0032870 GO:0035690 GO:0042221 GO:0042493 GO:0042742 GO:0043207 GO:0045087 GO:0045088 GO:0045089 GO:0045892 GO:0045934 GO:0046677 GO:0048518 GO:0048519 GO:0048523 GO:0048583 GO:0048584 GO:0048831 GO:0050776 GO:0050778 GO:0050789 GO:0050793 GO:0050794 GO:0050832 GO:0050896 GO:0051093 GO:0051171 GO:0051172 GO:0051239 GO:0051241 GO:0051252 GO:0051253 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0065007 GO:0070887 GO:0071229 GO:0071236 GO:0071310 GO:0071395 GO:0071407 GO:0071446 GO:0071495 GO:0080090 GO:0080134 GO:0098542 GO:0140110 GO:1900055 GO:1900056 GO:1901698 GO:1901700 GO:1901701 GO:1902679 GO:1903506 GO:1903507 GO:1905622 GO:2000024 GO:2000026 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WRKY

Protein Analysis

340

Amino Acids

37.73

Weight (kDa)

6.41

Isoelectric Point (pI)

50.64

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WRKY	PF03106	164 - 223	6.3e-23	WRKY DNA -binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001288)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G22570
fragaria_vesca	FvH4_6g09650 FvH4_6g09710 FvH4_6g09710 FvH4_6g09730 FvH4_6g09740 FvH4_6g09740 FvH4_6g09740 FvH4_6g09740 FvH4_6g09740 FvH4_6g09740 FvH4_6g09750 FvH4_6g09760
malus_domestica	MD04G1175500.v1.1 MD04G1175600.v1.1 MD12G1189200.v1.1 MD12G1189400.v1.1 MD12G1189600.v1.1 MD12G1189700.v1.1 MD12G1189900.v1.1
prunus_persica	Prupe.6G294900_v2.0.a1 Prupe.6G295000_v2.0.a1 Prupe.6G295100_v2.0.a1
pyrus_communis	pycom04g15480 pycom04g15490 pycom12g17650 pycom12g17690 pycom12g17700 pycom12g17730
rosa_chinensis	RchiOBHm_Chr3g0460351 RchiOBHm_Chr3g0460361
rosa_laevigata	RLG00000025018 RLG00000025019 RLG00000025027 RLG00000025113
rosa_multiflora	Rmu_sc0000449.1_g000005 Rmu_sc0004267.1_g000019
rosa_roxburghii	Rroxscaffold_6G00419560 Rroxscaffold_6G00419590
rosa_rugosa	Rorug03G0033500 Rorug03G0033500 Rorug03G0041400 Rorug03G0041800 Rorug03G0042000
rosa_samantha	Rh3AG100000 Rh3AG100100 Rh3BG094800 Rh3BG103900 Rh3BG104000 Rh3CG105700 Rh3CG105800 Rh3DG105300 Rh3DG105400
rosa_wichuraiana	Rw3G008480 Rw3G008490

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1006
AccB1I	GGYRCC	1 cut(s)	656
AclWI	GGATC	2 cut(s)	55, 429
AcoI	YGGCCR	1 cut(s)	14
AcuI	CTGAAG	2 cut(s)	168, 393
AcvI	CACGTG	1 cut(s)	666
AdeI	CACNNNGTG	1 cut(s)	862
AfaI	GTAC	1 cut(s)	888
AfiI	CCNNNNNNNGG	2 cut(s)	947, 948
AflIII	ACRYGT	2 cut(s)	663, 987
AgsI	TTSAA	6 cut(s)	245, 411, 418, 611, 620, 729
AluBI	AGCT	2 cut(s)	76, 359
AluI	AGCT	2 cut(s)	76, 359
Alw21I	GWGCWC	1 cut(s)	569
Alw26I	GTCTC	2 cut(s)	465, 666
Alw44I	GTGCAC	1 cut(s)	565
AlwI	GGATC	2 cut(s)	55, 429
AoxI	GGCC	2 cut(s)	14, 938
ApaI	GGGCCC	1 cut(s)	942
ApaLI	GTGCAC	1 cut(s)	565
ApeKI	GCWGC	2 cut(s)	356, 359
Asp700I	GAANNNNTTC	3 cut(s)	406, 430, 451
AspS9I	GGNCC	2 cut(s)	938, 939
AsuHPI	GGTGA	1 cut(s)	58
AsuII	TTCGAA	1 cut(s)	640
BaeGI	GKGCMC	2 cut(s)	569, 942
BanI	GGYRCC	1 cut(s)	656
BanII	GRGCYC	1 cut(s)	942
BarI	GAAGNNNNNNTAC	2 cut(s)	391, 423
BbrPI	CACGTG	1 cut(s)	666
BbsI	GAAGAC	2 cut(s)	381, 1004
Bbv12I	GWGCWC	1 cut(s)	569
BbvI	GCAGC	2 cut(s)	346, 368
BccI	CCATC	2 cut(s)	43, 128
BceAI	ACGGC	2 cut(s)	905, 936
BclI	TGATCA	3 cut(s)	217, 853, 895
BcoDI	GTCTC	2 cut(s)	465, 666
BfaI	CTAG	3 cut(s)	108, 468, 717
BisI	GCNGC	2 cut(s)	357, 360
BlsI	GCNGC	2 cut(s)	358, 361
BmgT120I	GGNCC	2 cut(s)	938, 939
BmiI	GGNNCC	2 cut(s)	658, 940
BmsI	GCATC	2 cut(s)	553, 787
BpiI	GAAGAC	2 cut(s)	381, 1004
BplI	GAGNNNNNCTC	2 cut(s)	663, 695
Bpu14I	TTCGAA	1 cut(s)	640
Bsa29I	ATCGAT	1 cut(s)	966
BsaAI	YACGTR	1 cut(s)	666
BsaJI	CCNNGG	3 cut(s)	64, 548, 942
BsaXI	ACNNNNNCTCC	1 cut(s)	26
Bsc4I	CCNNNNNNNGG	2 cut(s)	947, 948
Bse1I	ACTGG	3 cut(s)	632, 699, 761
BseCI	ATCGAT	1 cut(s)	966
BseDI	CCNNGG	3 cut(s)	64, 548, 942
BseGI	GGATG	3 cut(s)	544, 625, 778
BseLI	CCNNNNNNNGG	2 cut(s)	947, 948
BseMII	CTCAG	1 cut(s)	375
BseNI	ACTGG	3 cut(s)	632, 699, 761
BseRI	GAGGAG	2 cut(s)	68, 401
BseSI	GKGCMC	2 cut(s)	569, 942
BseXI	GCAGC	2 cut(s)	346, 368
BsgI	GTGCAG	1 cut(s)	688
BshFI	GGCC	2 cut(s)	16, 940
BshNI	GGYRCC	1 cut(s)	656
BshVI	ATCGAT	1 cut(s)	966
BsiHKAI	GWGCWC	1 cut(s)	569
BsiSI	CCGG	1 cut(s)	17
BsiWI	CGTACG	1 cut(s)	886
BslFI	GGGAC	1 cut(s)	478
BslI	CCNNNNNNNGG	2 cut(s)	947, 948
BsmAI	GTCTC	2 cut(s)	465, 666
BsmFI	GGGAC	1 cut(s)	478
BsnI	GGCC	2 cut(s)	16, 940
Bsp119I	TTCGAA	1 cut(s)	640
Bsp120I	GGGCCC	1 cut(s)	938
Bsp1286I	GDGCHC	2 cut(s)	569, 942
Bsp19I	CCATGG	1 cut(s)	942
BspANI	GGCC	2 cut(s)	16, 940
BspCNI	CTCAG	1 cut(s)	376
BspDI	ATCGAT	1 cut(s)	966
BspHI	TCATGA	1 cut(s)	130
BspLI	GGNNCC	2 cut(s)	658, 940
BspPI	GGATC	2 cut(s)	55, 429
BspT104I	TTCGAA	1 cut(s)	640
BspT107I	GGYRCC	1 cut(s)	656
BsrI	ACTGG	3 cut(s)	632, 699, 761
BssECI	CCNNGG	3 cut(s)	64, 548, 942
BssT1I	CCWWGG	3 cut(s)	64, 548, 942
Bst4CI	ACNGT	3 cut(s)	293, 395, 497
Bst6I	CTCTTC	1 cut(s)	421
BstBAI	YACGTR	1 cut(s)	666
BstBI	TTCGAA	1 cut(s)	640
BstC8I	GCNNGC	1 cut(s)	78
BstDEI	CTNAG	2 cut(s)	89, 384
BstDSI	CCRYGG	1 cut(s)	942
BstF5I	GGATG	3 cut(s)	544, 625, 778
BstMAI	GTCTC	2 cut(s)	465, 666
BstMWI	GCNNNNNNNGC	1 cut(s)	797
BstNSI	RCATGY	1 cut(s)	991
BstSLI	GKGCMC	2 cut(s)	569, 942
BstV1I	GCAGC	2 cut(s)	346, 368
BstV2I	GAAGAC	2 cut(s)	381, 1004
Bsu15I	ATCGAT	1 cut(s)	966
BsuRI	GGCC	2 cut(s)	16, 940
BsuTUI	ATCGAT	1 cut(s)	966
BtgI	CCRYGG	1 cut(s)	942
BtsCI	GGATG	3 cut(s)	544, 625, 778
BtsIMutI	CAGTG	1 cut(s)	639
Cac8I	GCNNGC	1 cut(s)	78
CciI	TCATGA	1 cut(s)	130
Cfr13I	GGNCC	2 cut(s)	938, 939
ClaI	ATCGAT	1 cut(s)	966
Csp6I	GTAC	1 cut(s)	887
CviAII	CATG	8 cut(s)	131, 347, 353, 500, 504, 911, 943, 988
CviJI	RGCY	7 cut(s)	16, 76, 98, 146, 359, 370, 940
CviKI_1	RGCY	7 cut(s)	16, 76, 98, 146, 359, 370, 940
CviQI	GTAC	1 cut(s)	887
DdeI	CTNAG	2 cut(s)	89, 384
DraI	TTTAAA	1 cut(s)	201
DraIII	CACNNNGTG	1 cut(s)	862
DrdI	GACNNNNNNGTC	1 cut(s)	1006
DseDI	GACNNNNNNGTC	1 cut(s)	1006
EaeI	YGGCCR	1 cut(s)	14
Eam1104I	CTCTTC	1 cut(s)	421
EarI	CTCTTC	1 cut(s)	421
Eco130I	CCWWGG	3 cut(s)	64, 548, 942
Eco24I	GRGCYC	1 cut(s)	942
Eco57I	CTGAAG	2 cut(s)	168, 393
Eco72I	CACGTG	1 cut(s)	666
EcoT14I	CCWWGG	3 cut(s)	64, 548, 942
EcoT22I	ATGCAT	1 cut(s)	505
EcoT38I	GRGCYC	1 cut(s)	942
ErhI	CCWWGG	3 cut(s)	64, 548, 942
FaeI	CATG	8 cut(s)	134, 350, 356, 503, 507, 914, 946, 991
FalI	AAGNNNNNCTT	4 cut(s)	418, 450, 436, 468
FaqI	GGGAC	1 cut(s)	478
FatI	CATG	8 cut(s)	130, 346, 352, 499, 503, 910, 942, 987
FbaI	TGATCA	3 cut(s)	217, 853, 895
Fnu4HI	GCNGC	2 cut(s)	357, 360
FokI	GGATG	3 cut(s)	531, 612, 765
FriOI	GRGCYC	1 cut(s)	942
Fsp4HI	GCNGC	2 cut(s)	357, 360
FspBI	CTAG	3 cut(s)	108, 468, 717
GluI	GCNGC	2 cut(s)	357, 360
HaeIII	GGCC	2 cut(s)	16, 940
HapII	CCGG	1 cut(s)	17
Hin1II	CATG	8 cut(s)	134, 350, 356, 503, 507, 914, 946, 991
HincII	GTYRAC	1 cut(s)	762
HindII	GTYRAC	1 cut(s)	762
HinfI	GANTC	7 cut(s)	5, 22, 212, 241, 722, 929, 1007
HpaII	CCGG	1 cut(s)	17
HphI	GGTGA	1 cut(s)	58
Hpy166II	GTNNAC	2 cut(s)	567, 762
Hpy188I	TCNGA	4 cut(s)	217, 240, 691, 918
Hpy188III	TCNNGA	3 cut(s)	131, 221, 620
Hpy8I	GTNNAC	2 cut(s)	567, 762
Hpy99I	CGWCG	1 cut(s)	13
HpyAV	CCTTC	4 cut(s)	143, 202, 424, 805
HpyCH4III	ACNGT	3 cut(s)	293, 395, 497
HpyCH4IV	ACGT	2 cut(s)	606, 665
HpyCH4V	TGCA	9 cut(s)	234, 320, 356, 503, 567, 591, 669, 708, 845
HpyF10VI	GCNNNNNNNGC	1 cut(s)	797
HpyF3I	CTNAG	2 cut(s)	89, 384
HpySE526I	ACGT	2 cut(s)	606, 665
Hsp92II	CATG	8 cut(s)	134, 350, 356, 503, 507, 914, 946, 991
Ksp22I	TGATCA	3 cut(s)	217, 853, 895
LpnPI	CCDG	9 cut(s)	30, 62, 384, 447, 492, 645, 680, 742, 921
Lsp1109I	GCAGC	2 cut(s)	346, 368
LweI	GCATC	2 cut(s)	553, 787
MaeI	CTAG	3 cut(s)	108, 468, 717
MaeII	ACGT	2 cut(s)	606, 665
MaeIII	GTNAC	3 cut(s)	111, 287, 862
MboII	GAAGA	8 cut(s)	386, 438, 456, 717, 830, 848, 989, 1009
MhlI	GDGCHC	2 cut(s)	569, 942
MlyI	GAGTC	5 cut(s)	14, 31, 221, 938, 1016
MnlI	CCTC	7 cut(s)	36, 46, 251, 379, 422, 689, 943
Mph1103I	ATGCAT	1 cut(s)	505
MroXI	GAANNNNTTC	3 cut(s)	406, 430, 451
MseI	TTAA	5 cut(s)	159, 200, 366, 813, 831
MslI	CAYNNNNRTG	1 cut(s)	351
MspA1I	CMGCKG	1 cut(s)	359
MspI	CCGG	1 cut(s)	17
MwoI	GCNNNNNNNGC	1 cut(s)	797
NcoI	CCATGG	1 cut(s)	942
NlaIII	CATG	8 cut(s)	134, 350, 356, 503, 507, 914, 946, 991
NlaIV	GGNNCC	2 cut(s)	658, 940
NmuCI	GTSAC	2 cut(s)	287, 862
NsiI	ATGCAT	1 cut(s)	505
NspI	RCATGY	1 cut(s)	991
NspV	TTCGAA	1 cut(s)	640
PagI	TCATGA	1 cut(s)	130
PciI	ACATGT	1 cut(s)	987
PdmI	GAANNNNTTC	3 cut(s)	406, 430, 451
PfeI	GAWTC	2 cut(s)	241, 722
Pfl23II	CGTACG	1 cut(s)	886
PkrI	GCNGC	2 cut(s)	358, 361
PleI	GAGTC	5 cut(s)	13, 30, 220, 937, 1015
PmaCI	CACGTG	1 cut(s)	666
PmlI	CACGTG	1 cut(s)	666
PpsI	GAGTC	5 cut(s)	13, 30, 220, 937, 1015
Ppu21I	YACGTR	1 cut(s)	666
PscI	ACATGT	1 cut(s)	987
PspCI	CACGTG	1 cut(s)	666
PspLI	CGTACG	1 cut(s)	886
PspN4I	GGNNCC	2 cut(s)	658, 940
PspOMI	GGGCCC	1 cut(s)	938
PspPI	GGNCC	2 cut(s)	938, 939
PvuII	CAGCTG	1 cut(s)	359
RsaI	GTAC	1 cut(s)	888
RsaNI	GTAC	1 cut(s)	887
RseI	CAYNNNNRTG	1 cut(s)	351
SaqAI	TTAA	5 cut(s)	159, 200, 366, 813, 831
SatI	GCNGC	2 cut(s)	357, 360
Sau96I	GGNCC	2 cut(s)	938, 939
SchI	GAGTC	5 cut(s)	14, 31, 221, 938, 1016
SduI	GDGCHC	2 cut(s)	569, 942
SfaNI	GCATC	2 cut(s)	553, 787
SfuI	TTCGAA	1 cut(s)	640
SmiMI	CAYNNNNRTG	1 cut(s)	351
SspMI	CTAG	3 cut(s)	108, 468, 717
StyI	CCWWGG	3 cut(s)	64, 548, 942
TaaI	ACNGT	3 cut(s)	293, 395, 497
TaiI	ACGT	2 cut(s)	609, 668
TaqI	TCGA	5 cut(s)	424, 640, 927, 966, 1005
TfiI	GAWTC	2 cut(s)	241, 722
Tru1I	TTAA	5 cut(s)	159, 200, 366, 813, 831
Tru9I	TTAA	5 cut(s)	159, 200, 366, 813, 831
TscAI	CASTG	1 cut(s)	639
TseFI	GTSAC	2 cut(s)	287, 862
TseI	GCWGC	2 cut(s)	356, 359
Tsp45I	GTSAC	2 cut(s)	287, 862
TspDTI	ATGAA	4 cut(s)	724, 792, 945, 990
TspGWI	ACGGA	1 cut(s)	874
TspRI	CASTG	1 cut(s)	639
VneI	GTGCAC	1 cut(s)	565
XceI	RCATGY	1 cut(s)	991
XmnI	GAANNNNTTC	3 cut(s)	406, 430, 451
XspI	CTAG	3 cut(s)	108, 468, 717
Zsp2I	ATGCAT	1 cut(s)	505

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.