Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0001288

Physical Location & Seq

Genomic Coordinates Reverse (-)

27213135 .. 27215427

Gene Structure 2293 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1189700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 999 bp

ATGGAGTGGTCTTGGCCGGAAAGTGTGCTCTCAAATAGGGAGAGGGTGGTGGAGGAGTTGATCCAAGGGCGTGAACTGGCAAGTCAACTTAGTAAGGCTTTTGCTAATAGATCACTTGTTGTTAATGGTGATGATGGGGGATCGGCTGAAGGTATTGTTAATAAGATTTTGGGGTCATTTGTGAATACCCTTTTGATTATAAATGGGAAGGAGTCTGACCAGGAGTTTGTTTCTGATCAGATTCAAGGAAATAGTAGTGGAGTCAGTGGTGGTAGTACTGGTGTTAGTGACGGTCGTGGTGGTGGTGCATATTCATCATCTTGGGATGCTAATCATGATCATGCAGCTAATATTAAGTCTGAAGATTATAATGAGGAGGAGATCAGTTGTAAGAGCACTTCAACCTTCAAGGATCGAAGAGGTTCCTACAAGAGAAGAAAGGCTTCACACTCTTGGACTACAGACACTCCTGCTTCGAACATTGATGACAGTCATGCATGGAGAAAGTACGGACAGAAAGTTATCCACAATGCCAAGCATCCAAGGAACTACTTTAGATGCACTCACAAATACAATCAAGCGTGCAAAGCAACCAAGCAAGTGCAACAAGTTCAAGATCATCCACCAGTGTTTCGAACCACATATTATGGCACCCACACGTGCAGAGACTACCCCAAAGCTTCTGAACTGGTCTTGGATTGCACTAGTCCTAGAGATTCCTCAAAATTCATTAGCTTTGACAACGCCAACTGTTTGGAAAACAAACAAGAGCACTTCACATCCTTCGCTTCATCATCAGTTAAAGAAGAACTGATCGTCAAGGCAACAAGTGATCATACGGTGACAAGCCACAACAACCATTCGTCACCAAGTGATTATCTACTGTCGCATGATGATCTGATGGCATTCGATTCCTTTTGGCCCATGAGCGAGTTTTCATCAAGCATTGATCAGTATGATCGTGACGATGTGTTTTTGAAGATGATTGCCGAGTCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0002218 GO:0002252 GO:0002253 GO:0002376 GO:0002682 GO:0002684 GO:0003674 GO:0003700 GO:0006355 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009627 GO:0009682 GO:0009719 GO:0009725 GO:0009751 GO:0009753 GO:0009755 GO:0009814 GO:0009862 GO:0009863 GO:0009864 GO:0009867 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010200 GO:0010243 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0014070 GO:0019219 GO:0019222 GO:0023052 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031347 GO:0031349 GO:0032870 GO:0035690 GO:0042221 GO:0042493 GO:0042742 GO:0043207 GO:0045087 GO:0045088 GO:0045089 GO:0045892 GO:0045934 GO:0046677 GO:0048518 GO:0048519 GO:0048523 GO:0048583 GO:0048584 GO:0048831 GO:0050776 GO:0050778 GO:0050789 GO:0050793 GO:0050794 GO:0050832 GO:0050896 GO:0051093 GO:0051171 GO:0051172 GO:0051239 GO:0051241 GO:0051252 GO:0051253 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0065007 GO:0070887 GO:0071229 GO:0071236 GO:0071310 GO:0071395 GO:0071407 GO:0071446 GO:0071495 GO:0080090 GO:0080134 GO:0098542 GO:0140110 GO:1900055 GO:1900056 GO:1901698 GO:1901700 GO:1901701 GO:1902679 GO:1903506 GO:1903507 GO:1905622 GO:2000024 GO:2000026 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WRKY

Protein Analysis

333

Amino Acids

37.18

Weight (kDa)

5.77

Isoelectric Point (pI)

49.4

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WRKY	PF03106	162 - 221	4e-22	WRKY DNA -binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001288)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G22570
fragaria_vesca	FvH4_6g09650 FvH4_6g09710 FvH4_6g09710 FvH4_6g09730 FvH4_6g09740 FvH4_6g09740 FvH4_6g09740 FvH4_6g09740 FvH4_6g09740 FvH4_6g09740 FvH4_6g09750 FvH4_6g09760
malus_domestica	MD04G1175500.v1.1 MD04G1175600.v1.1 MD12G1189200.v1.1 MD12G1189400.v1.1 MD12G1189600.v1.1 MD12G1189700.v1.1 MD12G1189900.v1.1
prunus_persica	Prupe.6G294900_v2.0.a1 Prupe.6G295000_v2.0.a1 Prupe.6G295100_v2.0.a1
pyrus_communis	pycom04g15480 pycom04g15490 pycom12g17650 pycom12g17690 pycom12g17700 pycom12g17730
rosa_chinensis	RchiOBHm_Chr3g0460351 RchiOBHm_Chr3g0460361
rosa_laevigata	RLG00000025018 RLG00000025019 RLG00000025027 RLG00000025113
rosa_multiflora	Rmu_sc0000449.1_g000005 Rmu_sc0004267.1_g000019
rosa_roxburghii	Rroxscaffold_6G00419560 Rroxscaffold_6G00419590
rosa_rugosa	Rorug03G0033500 Rorug03G0033500 Rorug03G0041400 Rorug03G0041800 Rorug03G0042000
rosa_samantha	Rh3AG100000 Rh3AG100100 Rh3BG094800 Rh3BG103900 Rh3BG104000 Rh3CG105700 Rh3CG105800 Rh3DG105300 Rh3DG105400
rosa_wichuraiana	Rw3G008480 Rw3G008490

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	200, 369
AccB1I	GGYRCC	1 cut(s)	650
AclWI	GGATC	3 cut(s)	55, 148, 420
AcoI	YGGCCR	1 cut(s)	14
AcsI	RAATTY	1 cut(s)	725
AcuI	CTGAAG	2 cut(s)	168, 381
AcvI	CACGTG	1 cut(s)	660
AdeI	CACNNNGTG	1 cut(s)	872
AfaI	GTAC	2 cut(s)	277, 509
AflIII	ACRYGT	1 cut(s)	657
AgsI	TTSAA	5 cut(s)	245, 402, 409, 614, 979
AhlI	ACTAGT	1 cut(s)	704
AjnI	CCWGG	1 cut(s)	219
AluBI	AGCT	3 cut(s)	347, 680, 735
AluI	AGCT	3 cut(s)	347, 680, 735
Alw21I	GWGCWC	3 cut(s)	30, 398, 774
Alw26I	GTCTC	1 cut(s)	660
AlwI	GGATC	3 cut(s)	55, 148, 420
AoxI	GGCC	2 cut(s)	14, 920
ApeKI	GCWGC	1 cut(s)	344
ApoI	RAATTY	1 cut(s)	725
ArsI	GACNNNNNNTTYG	2 cut(s)	209, 241
Asp700I	GAANNNNTTC	2 cut(s)	421, 442
AspS9I	GGNCC	1 cut(s)	921
AsuHPI	GGTGA	3 cut(s)	140, 853, 858
AsuII	TTCGAA	2 cut(s)	476, 634
BanI	GGYRCC	1 cut(s)	650
BarI	GAAGNNNNNNTAC	2 cut(s)	382, 414
BbrPI	CACGTG	1 cut(s)	660
Bbv12I	GWGCWC	3 cut(s)	30, 398, 774
BbvI	GCAGC	1 cut(s)	356
BccI	CCATC	2 cut(s)	128, 895
BciT130I	CCWGG	1 cut(s)	221
BclI	TGATCA	4 cut(s)	235, 337, 832, 949
BcoDI	GTCTC	1 cut(s)	660
BcuI	ACTAGT	1 cut(s)	704
BfaI	CTAG	2 cut(s)	705, 711
BfmI	CTRYAG	1 cut(s)	459
BisI	GCNGC	1 cut(s)	345
BlsI	GCNGC	1 cut(s)	346
BmcAI	AGTACT	1 cut(s)	277
Bme1390I	CCNGG	1 cut(s)	221
BmgT120I	GGNCC	1 cut(s)	921
BmiI	GGNNCC	2 cut(s)	424, 652
BmrFI	CCNGG	1 cut(s)	221
BmsI	GCATC	3 cut(s)	316, 547, 548
Bpu14I	TTCGAA	2 cut(s)	476, 634
BsaAI	YACGTR	1 cut(s)	660
BsaBI	GATNNNNATC	1 cut(s)	330
BsaJI	CCNNGG	2 cut(s)	64, 542
BsaXI	ACNNNNNCTCC	2 cut(s)	451, 481
Bse1I	ACTGG	4 cut(s)	81, 283, 626, 693
Bse8I	GATNNNNATC	1 cut(s)	330
BseBI	CCWGG	1 cut(s)	221
BseDI	CCNNGG	2 cut(s)	64, 542
BseGI	GGATG	4 cut(s)	331, 538, 619, 779
BseJI	GATNNNNATC	1 cut(s)	330
BseNI	ACTGG	4 cut(s)	81, 283, 626, 693
BseRI	GAGGAG	3 cut(s)	68, 389, 392
BseXI	GCAGC	1 cut(s)	356
BsgI	GTGCAG	1 cut(s)	682
Bsh1285I	CGRYCG	1 cut(s)	295
BshFI	GGCC	2 cut(s)	16, 922
BshNI	GGYRCC	1 cut(s)	650
BsiEI	CGRYCG	1 cut(s)	295
BsiHKAI	GWGCWC	3 cut(s)	30, 398, 774
BsiSI	CCGG	1 cut(s)	17
BsmAI	GTCTC	1 cut(s)	660
BsmI	GAATGC	1 cut(s)	905
BsnI	GGCC	2 cut(s)	16, 922
Bsp119I	TTCGAA	2 cut(s)	476, 634
Bsp1286I	GDGCHC	3 cut(s)	30, 398, 774
BspANI	GGCC	2 cut(s)	16, 922
BspHI	TCATGA	1 cut(s)	334
BspLI	GGNNCC	2 cut(s)	424, 652
BspPI	GGATC	3 cut(s)	55, 148, 420
BspT104I	TTCGAA	2 cut(s)	476, 634
BspT107I	GGYRCC	1 cut(s)	650
BsrI	ACTGG	4 cut(s)	81, 283, 626, 693
BssECI	CCNNGG	2 cut(s)	64, 542
BssT1I	CCWWGG	2 cut(s)	64, 542
Bst2UI	CCWGG	1 cut(s)	221
Bst4CI	ACNGT	5 cut(s)	293, 491, 752, 841, 885
Bst6I	CTCTTC	1 cut(s)	412
BstBAI	YACGTR	1 cut(s)	660
BstBI	TTCGAA	2 cut(s)	476, 634
BstC8I	GCNNGC	1 cut(s)	583
BstDEI	CTNAG	1 cut(s)	89
BstF5I	GGATG	4 cut(s)	331, 538, 619, 779
BstMAI	GTCTC	1 cut(s)	660
BstMCI	CGRYCG	1 cut(s)	295
BstMWI	GCNNNNNNNGC	1 cut(s)	587
BstNI	CCWGG	1 cut(s)	221
BstSCI	CCNGG	1 cut(s)	219
BstSFI	CTRYAG	1 cut(s)	459
BstV1I	GCAGC	1 cut(s)	356
BstXI	CCANNNNNNTGG	1 cut(s)	754
BsuRI	GGCC	2 cut(s)	16, 922
BtsCI	GGATG	4 cut(s)	331, 538, 619, 779
BtsIMutI	CAGTG	2 cut(s)	271, 633
Cac8I	GCNNGC	1 cut(s)	583
CciI	TCATGA	1 cut(s)	334
Cfr13I	GGNCC	1 cut(s)	921
Csp6I	GTAC	2 cut(s)	276, 508
CviAII	CATG	6 cut(s)	335, 341, 494, 498, 890, 925
CviJI	RGCY	9 cut(s)	16, 98, 146, 347, 443, 680, 735, 849, 922
CviKI_1	RGCY	9 cut(s)	16, 98, 146, 347, 443, 680, 735, 849, 922
CviQI	GTAC	2 cut(s)	276, 508
DdeI	CTNAG	1 cut(s)	89
DraIII	CACNNNGTG	1 cut(s)	872
EaeI	YGGCCR	1 cut(s)	14
Eam1104I	CTCTTC	1 cut(s)	412
EarI	CTCTTC	1 cut(s)	412
Eco130I	CCWWGG	2 cut(s)	64, 542
Eco57I	CTGAAG	2 cut(s)	168, 381
Eco72I	CACGTG	1 cut(s)	660
EcoRII	CCWGG	1 cut(s)	219
EcoT14I	CCWWGG	2 cut(s)	64, 542
EcoT22I	ATGCAT	1 cut(s)	499
ErhI	CCWWGG	2 cut(s)	64, 542
FaeI	CATG	6 cut(s)	338, 344, 497, 501, 893, 928
FalI	AAGNNNNNCTT	2 cut(s)	427, 459
FatI	CATG	6 cut(s)	334, 340, 493, 497, 889, 924
FbaI	TGATCA	4 cut(s)	235, 337, 832, 949
Fnu4HI	GCNGC	1 cut(s)	345
FokI	GGATG	4 cut(s)	338, 525, 606, 766
Fsp4HI	GCNGC	1 cut(s)	345
FspBI	CTAG	2 cut(s)	705, 711
GluI	GCNGC	1 cut(s)	345
HaeIII	GGCC	2 cut(s)	16, 922
HapII	CCGG	1 cut(s)	17
Hin1II	CATG	6 cut(s)	338, 344, 497, 501, 893, 928
HincII	GTYRAC	1 cut(s)	86
HindII	GTYRAC	1 cut(s)	86
HindIII	AAGCTT	1 cut(s)	678
HinfI	GANTC	6 cut(s)	212, 241, 261, 716, 911, 992
HpaII	CCGG	1 cut(s)	17
HphI	GGTGA	3 cut(s)	140, 853, 858
Hpy166II	GTNNAC	2 cut(s)	74, 86
Hpy188I	TCNGA	6 cut(s)	217, 235, 240, 361, 685, 900
Hpy188III	TCNNGA	3 cut(s)	335, 614, 962
Hpy8I	GTNNAC	2 cut(s)	74, 86
HpyAV	CCTTC	4 cut(s)	143, 202, 415, 793
HpyCH4III	ACNGT	5 cut(s)	293, 491, 752, 841, 885
HpyCH4IV	ACGT	1 cut(s)	659
HpyCH4V	TGCA	8 cut(s)	308, 344, 497, 561, 585, 604, 663, 702
HpyF10VI	GCNNNNNNNGC	1 cut(s)	587
HpyF3I	CTNAG	1 cut(s)	89
HpySE526I	ACGT	1 cut(s)	659
Hsp92II	CATG	6 cut(s)	338, 344, 497, 501, 893, 928
Ksp22I	TGATCA	4 cut(s)	235, 337, 832, 949
LpnPI	CCDG	8 cut(s)	30, 62, 206, 233, 264, 483, 639, 674
Lsp1109I	GCAGC	1 cut(s)	356
LweI	GCATC	3 cut(s)	316, 547, 548
MaeI	CTAG	2 cut(s)	705, 711
MaeII	ACGT	1 cut(s)	659
MaeIII	GTNAC	4 cut(s)	287, 841, 864, 962
MboII	GAAGA	5 cut(s)	374, 429, 447, 818, 991
MhlI	GDGCHC	3 cut(s)	30, 398, 774
MluCI	AATT	1 cut(s)	725
MlyI	GAGTC	2 cut(s)	221, 270
MnlI	CCTC	6 cut(s)	36, 46, 367, 370, 413, 730
Mph1103I	ATGCAT	1 cut(s)	499
MroXI	GAANNNNTTC	2 cut(s)	421, 442
MseI	TTAA	5 cut(s)	123, 159, 354, 801, 997
MslI	CAYNNNNRTG	1 cut(s)	339
MspI	CCGG	1 cut(s)	17
MspR9I	CCNGG	1 cut(s)	221
Mva1269I	GAATGC	1 cut(s)	905
MvaI	CCWGG	1 cut(s)	221
MwoI	GCNNNNNNNGC	1 cut(s)	587
NlaIII	CATG	6 cut(s)	338, 344, 497, 501, 893, 928
NlaIV	GGNNCC	2 cut(s)	424, 652
NmuCI	GTSAC	4 cut(s)	287, 841, 864, 962
NsiI	ATGCAT	1 cut(s)	499
NspV	TTCGAA	2 cut(s)	476, 634
PagI	TCATGA	1 cut(s)	334
PctI	GAATGC	1 cut(s)	905
PdmI	GAANNNNTTC	2 cut(s)	421, 442
PfeI	GAWTC	3 cut(s)	241, 716, 911
PkrI	GCNGC	1 cut(s)	346
PleI	GAGTC	2 cut(s)	220, 269
PmaCI	CACGTG	1 cut(s)	660
PmlI	CACGTG	1 cut(s)	660
PpsI	GAGTC	2 cut(s)	220, 269
Ppu21I	YACGTR	1 cut(s)	660
PsiI	TTATAA	2 cut(s)	200, 369
Psp6I	CCWGG	1 cut(s)	219
PspCI	CACGTG	1 cut(s)	660
PspGI	CCWGG	1 cut(s)	219
PspN4I	GGNNCC	2 cut(s)	424, 652
PspPI	GGNCC	1 cut(s)	921
RsaI	GTAC	2 cut(s)	277, 509
RsaNI	GTAC	2 cut(s)	276, 508
RseI	CAYNNNNRTG	1 cut(s)	339
SaqAI	TTAA	5 cut(s)	123, 159, 354, 801, 997
SatI	GCNGC	1 cut(s)	345
Sau96I	GGNCC	1 cut(s)	921
ScaI	AGTACT	1 cut(s)	277
SchI	GAGTC	2 cut(s)	221, 270
ScrFI	CCNGG	1 cut(s)	221
SduI	GDGCHC	3 cut(s)	30, 398, 774
SetI	ASST	7 cut(s)	154, 349, 407, 424, 662, 682, 737
SfaNI	GCATC	3 cut(s)	316, 547, 548
SfcI	CTRYAG	1 cut(s)	459
SfuI	TTCGAA	2 cut(s)	476, 634
SmiMI	CAYNNNNRTG	1 cut(s)	339
SpeI	ACTAGT	1 cut(s)	704
Sse9I	AATT	1 cut(s)	725
SspI	AATATT	1 cut(s)	352
SspMI	CTAG	2 cut(s)	705, 711
StyD4I	CCNGG	1 cut(s)	219
StyI	CCWWGG	2 cut(s)	64, 542
TaaI	ACNGT	5 cut(s)	293, 491, 752, 841, 885
TaiI	ACGT	1 cut(s)	662
TaqI	TCGA	4 cut(s)	415, 476, 634, 909
TasI	AATT	1 cut(s)	725
TatI	WGTACW	1 cut(s)	275
TfiI	GAWTC	3 cut(s)	241, 716, 911
Tru1I	TTAA	5 cut(s)	123, 159, 354, 801, 997
Tru9I	TTAA	5 cut(s)	123, 159, 354, 801, 997
TscAI	CASTG	2 cut(s)	271, 633
TseFI	GTSAC	4 cut(s)	287, 841, 864, 962
TseI	GCWGC	1 cut(s)	344
Tsp45I	GTSAC	4 cut(s)	287, 841, 864, 962
TspDTI	ATGAA	4 cut(s)	303, 718, 780, 927
TspGWI	ACGGA	1 cut(s)	525
TspRI	CASTG	2 cut(s)	271, 633
XapI	RAATTY	1 cut(s)	725
XmnI	GAANNNNTTC	2 cut(s)	421, 442
XspI	CTAG	2 cut(s)	705, 711
ZrmI	AGTACT	1 cut(s)	277
Zsp2I	ATGCAT	1 cut(s)	499

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.