Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0001570

Physical Location & Seq

Genomic Coordinates Reverse (-)

27100296 .. 27104493

Gene Structure 4198 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1180100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1680 bp

ATGGGGAGCTCAGGATTCGACTGGAAACTGCCGGATCACCCTAAGTTGCCGAAGGGGAAGGTCATCGGACTCATCGTTTTGGATGGGTGGGGCGAGGCCAACGCCGACCAGTACAACTGCATCCATGTCGCCGAGACCCCCGTCATGGATTCGCTCAAGAAGGGTGCTCCTGAAAGATGGAGGTTAGTGAGAGCTCACGGAACTGCCGTGGGACTTCCCACAGAGGATGATATGGGCAATAGTGAAGTGGGTCACAATGCCCTCGGCGCCGGGCGTATTTTTGCCCAGGGTGCTAAGCTTGTTGATGCTGCACTTGCTTCTGGGAAAATTTTCGAGGGAGAAGGTTTTAAGTATGTTAAGGAATCCTTTGAGACTAACACATTGCATCTCATTGGTTTAATGAGTGATGGTGGAGTTCATTCTCGGCTTGATCAATTGCTATTGTTGCTTAAAGGAGCTAGCGAACAAGGTGCTAAAAAGATCCGTGTTCATATTCTCACTGATGGGCGTGATGTTCTGGACGGTTCAAGTGTGGGATTTGCAGAAATCCTTGAAAATGACCTTGCGAAATTGCGTGAGAAAGGTGTGGATGCACAGATTGCGTCTGGTGGTGGTCGTATGTATGTCACCATGGATCGTTATGAGAATGATTGGAGTGTTGTAAAACGAGGATGGGATGCCCAAGTTCTTGGTGAAGCTCCCTATAAATTTAAGAATGCAGTTGAAGCTATCAAGACGTTGAGGGCGGAACCAAACGCCAATGACCAGTATCTACCTCCTTTTGTTATTGTTGACGACAGTGGAAAGCCTGTTGGCCCAATCGTGGATGGTGATGCTGTTGTTACATTCAACTTCCGTGCGGACCGTATGGTTATGATTGCCAAGGCGCTTGAGTATGCCGATTTTGACAAGTTTGATCGAGTTCGAGTCCCAAAAATCCGTTATGCTGGTATGCTTCAGTATGATGGTGAGCTGAAGCTTCCAAGCCATTACCTTGTAGAACCCCCAGAGATTGATAGAACATCTGGTGAATATCTTACATATAACGGTGTCCGCACTTTTGCCTGCAGTGAGACTGTTAAGTTTGGCCATGTCACCTTCTTCTGGAACGGCAATCGCTCTGGTTATTTTAACGATAAAATGGAGGAGTATGTGGAAATTCCAAGCGATAGTGGAATCACATTCAACGTCCAGCCAAAAATGAAGGCAGTCGAGATTGCTGAAAAGGCTAGGGATGCTATTCTTAGTGGAAAGTTTGAGCAGGTACGTGTTAACCTTCCAAATGGCGACATGGTGGGGCACACAGGCGACATTGAGGCCACAATTGTTGCTTGCAAGGCTGCTGATGAAGCCGTCAAGATAATTCTTGATGCAATCGAGAAGGTTGGTGGAATTTACGTCGTCACTGCTGATCATGGTAACGCTGAGGATATGGTGAAGAGAAATAAGACTGGGCAACCTCTTCTCGACAAGAGTGGCAACATTCAAATTCTTACTTCCCATACTCTACAGCCAGTCCCAATTGCAATTGGAGGTCCTGGACTTGCACCCGGAGTCCGTTTCCGCAAGGATCTTCCTAGCGGCGGGCTGGCCAATGTTGCTGCAACTGTGATGAACCTGCATGGATTCCAGGCTCCTAGCGACTACGAGCCATCCCTCATCGAAGTTGTTGATAACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004619 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005829 GO:0005886 GO:0005911 GO:0006950 GO:0007275 GO:0008150 GO:0009266 GO:0009409 GO:0009506 GO:0009507 GO:0009536 GO:0009555 GO:0009628 GO:0009987 GO:0010035 GO:0010038 GO:0010118 GO:0016020 GO:0016853 GO:0016866 GO:0016868 GO:0030054 GO:0031967 GO:0031975 GO:0032501 GO:0032502 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0046537 GO:0046686 GO:0048046 GO:0048229 GO:0048856 GO:0050896 GO:0055044 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko00010 ko00260 ko00680 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 map00010 map00260 map00680 map01100 map01110 map01120 map01130 map01200 map01230

Pfam Domains

Protein Families

Metalloenzyme iPGM_N

Protein Analysis

560

Amino Acids

60.83

Weight (kDa)

5.44

Isoelectric Point (pI)

32.33

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Metalloenzyme	PF01676	22 - 544	7e-89	Metalloenzyme superfamily
iPGM_N	PF06415	101 - 322	1.1e-58	BPG-independent PGAM N-terminus (iPGM_N)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001570)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G09780 AT3G08590 AT3G08590
fragaria_vesca	FvH4_6g09180 FvH4_6g09180 FvH4_6g09180
malus_domestica	MD04G1180100.v1.1 MD12G1195300.v1.1
prunus_persica	Prupe.6G300000_v2.0.a1 Prupe.6G300000_v2.0.a1 Prupe.6G300000_v2.0.a1
pyrus_communis	pycom04g15930 pycom12g18160
rosa_chinensis	RchiOBHm_Chr2g0154971 RchiOBHm_Chr2g0154981 RchiOBHm_Chr3g0459381 RchiOBHm_Chr3g0467071 RchiOBHm_Chr5g0071081 RchiOBHm_Chr6g0301401
rosa_laevigata	RLG00000020806 RLG00000025085 RLG00000033543
rosa_multiflora	Rmu_sc0000240.1_g000005 Rmu_sc0001304.1_g000020 Rmu_sc0004018.1_g000005 Rmu_sc0007705.1_g000010 Rmu_sc0014417.1_g000008 Rmu_ssc0000065.1_g000007
rosa_roxburghii	Rroxscaffold_2G00122270 Rroxscaffold_3G00253790 Rroxscaffold_6G00420200
rosa_rugosa	Rorug03G0036100 Rorug03G0036200 Rorug03G0036200 Rorug05G0003600 Rorug05G0003600 Rorug05G0003700
rosa_samantha	Rh3AG094000 Rh3AG133200 Rh3BG154300 Rh3CG098200 Rh3CG308400 Rh3DG097700 Rh4CG280400 Rh5BG093900 Rh5BG235100 Rh6AG418000 Rh6CG431600
rosa_wichuraiana	Rw3G008090 Rw6G036180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	1252, 1626
AccB1I	GGYRCC	1 cut(s)	266
AciI	CCGC	6 cut(s)	746, 860, 1054, 1564, 1581, 1584
AclWI	GGATC	4 cut(s)	42, 475, 642, 1578
AcoI	YGGCCR	2 cut(s)	1087, 1590
AcsI	RAATTY	5 cut(s)	327, 707, 1158, 1392, 1488
AcuI	CTGAAG	2 cut(s)	941, 995
AcyI	GRCGYC	1 cut(s)	267
AfaI	GTAC	2 cut(s)	113, 1266
AfiI	CCNNNNNNNGG	4 cut(s)	145, 823, 1104, 1583
AflIII	ACRYGT	1 cut(s)	1267
AgsI	TTSAA	6 cut(s)	528, 554, 725, 850, 1186, 1487
AjnI	CCWGG	3 cut(s)	285, 1537, 1629
AluBI	AGCT	8 cut(s)	9, 194, 298, 458, 698, 728, 973, 979
AluI	AGCT	8 cut(s)	9, 194, 298, 458, 698, 728, 973, 979
Alw21I	GWGCWC	3 cut(s)	11, 169, 196
Alw26I	GTCTC	3 cut(s)	128, 365, 1067
AlwI	GGATC	4 cut(s)	42, 475, 642, 1578
AoxI	GGCC	5 cut(s)	96, 814, 1087, 1317, 1590
ApeKI	GCWGC	3 cut(s)	308, 1340, 1601
ApoI	RAATTY	5 cut(s)	327, 707, 1158, 1392, 1488
Asp700I	GAANNNNTTC	1 cut(s)	329
AspLEI	GCGC	2 cut(s)	269, 889
AspS9I	GGNCC	3 cut(s)	815, 862, 1535
AsuC2I	CCSGG	2 cut(s)	271, 1551
AsuHPI	GGTGA	8 cut(s)	29, 619, 704, 842, 980, 1040, 1087, 1447
AsuNHI	GCTAGC	1 cut(s)	458
AvaII	GGWCC	2 cut(s)	862, 1535
BaeGI	GKGCMC	1 cut(s)	1302
BalI	TGGCCA	2 cut(s)	1089, 1592
BanI	GGYRCC	1 cut(s)	266
BanII	GRGCYC	2 cut(s)	11, 196
Bbv12I	GWGCWC	3 cut(s)	11, 169, 196
BbvCI	CCTCAGC	1 cut(s)	1425
BbvI	GCAGC	3 cut(s)	295, 1327, 1588
BccI	CCATC	8 cut(s)	77, 171, 401, 497, 666, 821, 959, 1660
BceAI	ACGGC	3 cut(s)	191, 1126, 1337
BcgI	CGANNNNNNTGC	4 cut(s)	109, 143, 452, 486
BciT130I	CCWGG	3 cut(s)	287, 1539, 1631
BclI	TGATCA	2 cut(s)	430, 1411
BcnI	CCSGG	2 cut(s)	271, 1551
BcoDI	GTCTC	3 cut(s)	128, 365, 1067
BfaI	CTAG	5 cut(s)	459, 1230, 1578, 1638, 1678
BfmI	CTRYAG	2 cut(s)	1066, 1508
BfoI	RGCGCY	2 cut(s)	270, 890
BfuAI	ACCTGC	2 cut(s)	1252, 1626
BisI	GCNGC	4 cut(s)	309, 1341, 1582, 1602
BlpI	GCTNAGC	1 cut(s)	294
BlsI	GCNGC	4 cut(s)	310, 1342, 1583, 1603
Bme1390I	CCNGG	5 cut(s)	271, 287, 1539, 1551, 1631
Bme18I	GGWCC	2 cut(s)	862, 1535
BmgT120I	GGNCC	3 cut(s)	815, 862, 1535
BmiI	GGNNCC	3 cut(s)	268, 750, 1635
BmrFI	CCNGG	5 cut(s)	271, 287, 1539, 1551, 1631
BmrI	ACTGGG	1 cut(s)	1461
BmsI	GCATC	8 cut(s)	129, 295, 394, 580, 667, 823, 1225, 1360
BmtI	GCTAGC	1 cut(s)	462
BmuI	ACTGGG	1 cut(s)	1461
BoxI	GACNNNNGTC	1 cut(s)	140
Bpu10I	CCTNAGC	2 cut(s)	10, 1425
Bpu1102I	GCTNAGC	1 cut(s)	294
BpuEI	CTTGAG	2 cut(s)	140, 911
BpuMI	CCSGG	2 cut(s)	271, 1551
BsaAI	YACGTR	1 cut(s)	1268
BsaHI	GRCGYC	1 cut(s)	267
BsaI	GGTCTC	1 cut(s)	128
BsaJI	CCNNGG	6 cut(s)	207, 262, 285, 286, 630, 882
Bsc4I	CCNNNNNNNGG	4 cut(s)	145, 823, 1104, 1583
Bse1I	ACTGG	5 cut(s)	26, 109, 766, 1456, 1514
Bse3DI	GCAATG	1 cut(s)	380
BseBI	CCWGG	3 cut(s)	287, 1539, 1631
BseDI	CCNNGG	6 cut(s)	207, 262, 285, 286, 630, 882
BseGI	GGATG	9 cut(s)	88, 120, 232, 595, 677, 682, 832, 1240, 1652
BseLI	CCNNNNNNNGG	4 cut(s)	145, 823, 1104, 1583
BseMI	GCAATG	1 cut(s)	380
BseMII	CTCAG	2 cut(s)	24, 1416
BseNI	ACTGG	5 cut(s)	26, 109, 766, 1456, 1514
BseRI	GAGGAG	1 cut(s)	1160
BseSI	GKGCMC	1 cut(s)	1302
BseXI	GCAGC	3 cut(s)	295, 1327, 1588
BsgI	GTGCAG	1 cut(s)	294
BshFI	GGCC	5 cut(s)	98, 816, 1089, 1319, 1592
BshNI	GGYRCC	1 cut(s)	266
BsiHKAI	GWGCWC	3 cut(s)	11, 169, 196
BsiSI	CCGG	3 cut(s)	32, 270, 1551
BslFI	GGGAC	3 cut(s)	225, 914, 1502
BslI	CCNNNNNNNGG	4 cut(s)	145, 823, 1104, 1583
BsmAI	GTCTC	3 cut(s)	128, 365, 1067
BsmFI	GGGAC	3 cut(s)	225, 914, 1502
BsmI	GAATGC	1 cut(s)	721
BsnI	GGCC	5 cut(s)	98, 816, 1089, 1319, 1592
Bso31I	GGTCTC	1 cut(s)	128
Bsp1286I	GDGCHC	4 cut(s)	11, 169, 196, 1302
Bsp143I	GATC	7 cut(s)	34, 430, 480, 634, 916, 1411, 1570
Bsp1720I	GCTNAGC	1 cut(s)	294
Bsp19I	CCATGG	1 cut(s)	630
BspACI	CCGC	6 cut(s)	746, 860, 1054, 1564, 1581, 1584
BspANI	GGCC	5 cut(s)	98, 816, 1089, 1319, 1592
BspCNI	CTCAG	2 cut(s)	23, 1417
BspLI	GGNNCC	3 cut(s)	268, 750, 1635
BspMAI	CTGCAG	1 cut(s)	1070
BspMI	ACCTGC	2 cut(s)	1252, 1626
BspOI	GCTAGC	1 cut(s)	462
BspPI	GGATC	4 cut(s)	42, 475, 642, 1578
BspT107I	GGYRCC	1 cut(s)	266
BspTNI	GGTCTC	1 cut(s)	128
BsrDI	GCAATG	1 cut(s)	380
BsrI	ACTGG	5 cut(s)	26, 109, 766, 1456, 1514
BssECI	CCNNGG	6 cut(s)	207, 262, 285, 286, 630, 882
BssMI	GATC	7 cut(s)	34, 430, 480, 634, 916, 1411, 1570
BssNI	GRCGYC	1 cut(s)	267
BssT1I	CCWWGG	2 cut(s)	630, 882
Bst2UI	CCWGG	3 cut(s)	287, 1539, 1631
Bst4CI	ACNGT	6 cut(s)	524, 800, 866, 1049, 1078, 1609
Bst6I	CTCTTC	2 cut(s)	1433, 1467
BstACI	GRCGYC	1 cut(s)	267
BstAPI	GCANNNNNTGC	1 cut(s)	599
BstBAI	YACGTR	1 cut(s)	1268
BstC8I	GCNNGC	5 cut(s)	460, 1066, 1333, 1586, 1590
BstDEI	CTNAG	5 cut(s)	10, 42, 294, 1244, 1425
BstDSI	CCRYGG	2 cut(s)	207, 630
BstF5I	GGATG	9 cut(s)	88, 120, 232, 595, 677, 682, 832, 1240, 1652
BstH2I	RGCGCY	2 cut(s)	270, 890
BstHHI	GCGC	2 cut(s)	269, 889
BstKTI	GATC	7 cut(s)	37, 433, 483, 637, 919, 1414, 1573
BstMAI	GTCTC	3 cut(s)	128, 365, 1067
BstMBI	GATC	7 cut(s)	34, 430, 480, 634, 916, 1411, 1570
BstNI	CCWGG	3 cut(s)	287, 1539, 1631
BstPAI	GACNNNNGTC	1 cut(s)	140
BstSCI	CCNGG	5 cut(s)	269, 285, 1537, 1549, 1629
BstSFI	CTRYAG	2 cut(s)	1066, 1508
BstSLI	GKGCMC	1 cut(s)	1302
BstV1I	GCAGC	3 cut(s)	295, 1327, 1588
BstX2I	RGATCY	2 cut(s)	480, 1570
BstXI	CCANNNNNNTGG	1 cut(s)	689
BstYI	RGATCY	2 cut(s)	480, 1570
BsuRI	GGCC	5 cut(s)	98, 816, 1089, 1319, 1592
BtgI	CCRYGG	2 cut(s)	207, 630
BtsCI	GGATG	9 cut(s)	88, 120, 232, 595, 677, 682, 832, 1240, 1652
BtsI	GCAGTG	2 cut(s)	1075, 1404
BtsIMutI	CAGTG	4 cut(s)	498, 805, 1075, 1404
BveI	ACCTGC	2 cut(s)	1252, 1626
Cac8I	GCNNGC	5 cut(s)	460, 1066, 1333, 1586, 1590
CfoI	GCGC	2 cut(s)	269, 889
Cfr13I	GGNCC	3 cut(s)	815, 862, 1535
CpoI	CGGWCCG	1 cut(s)	862
CseI	GACGC	1 cut(s)	591
Csp6I	GTAC	2 cut(s)	112, 1265
CspCI	CAANNNNNGTGG	2 cut(s)	1309, 1344
CspI	CGGWCCG	1 cut(s)	862
CviAII	CATG	7 cut(s)	125, 145, 631, 1091, 1291, 1415, 1622
CviQI	GTAC	2 cut(s)	112, 1265
DdeI	CTNAG	5 cut(s)	10, 42, 294, 1244, 1425
DinI	GGCGCC	1 cut(s)	268
DpnI	GATC	7 cut(s)	36, 432, 482, 636, 918, 1413, 1572
DpnII	GATC	7 cut(s)	34, 430, 480, 634, 916, 1411, 1570
EaeI	YGGCCR	2 cut(s)	1087, 1590
Eam1104I	CTCTTC	2 cut(s)	1433, 1467
EarI	CTCTTC	2 cut(s)	1433, 1467
EciI	GGCGGA	1 cut(s)	761
Ecl136II	GAGCTC	2 cut(s)	9, 194
Eco130I	CCWWGG	2 cut(s)	630, 882
Eco24I	GRGCYC	2 cut(s)	11, 196
Eco31I	GGTCTC	1 cut(s)	128
Eco47I	GGWCC	2 cut(s)	862, 1535
Eco53kI	GAGCTC	2 cut(s)	9, 194
Eco57I	CTGAAG	2 cut(s)	941, 995
EcoICRI	GAGCTC	2 cut(s)	9, 194
EcoO109I	RGGNCCY	1 cut(s)	1535
EcoRII	CCWGG	3 cut(s)	285, 1537, 1629
EcoT14I	CCWWGG	2 cut(s)	630, 882
EcoT38I	GRGCYC	2 cut(s)	11, 196
EgeI	GGCGCC	1 cut(s)	268
EheI	GGCGCC	1 cut(s)	268
ErhI	CCWWGG	2 cut(s)	630, 882
FaeI	CATG	7 cut(s)	128, 148, 634, 1094, 1294, 1418, 1625
FalI	AAGNNNNNCTT	2 cut(s)	350, 382
FaqI	GGGAC	3 cut(s)	225, 914, 1502
FatI	CATG	7 cut(s)	124, 144, 630, 1090, 1290, 1414, 1621
FauI	CCCGC	1 cut(s)	1577
FbaI	TGATCA	2 cut(s)	430, 1411
Fnu4HI	GCNGC	4 cut(s)	309, 1341, 1582, 1602
FokI	GGATG	9 cut(s)	95, 107, 239, 602, 684, 689, 839, 1247, 1639
FriOI	GRGCYC	2 cut(s)	11, 196
Fsp4HI	GCNGC	4 cut(s)	309, 1341, 1582, 1602
FspBI	CTAG	5 cut(s)	459, 1230, 1578, 1638, 1678
GlaI	GCGC	2 cut(s)	268, 888
GluI	GCNGC	4 cut(s)	309, 1341, 1582, 1602
HaeII	RGCGCY	2 cut(s)	270, 890
HaeIII	GGCC	5 cut(s)	98, 816, 1089, 1319, 1592
HapII	CCGG	3 cut(s)	32, 270, 1551
HgaI	GACGC	1 cut(s)	591
HhaI	GCGC	2 cut(s)	269, 889
Hin1I	GRCGYC	1 cut(s)	267
Hin1II	CATG	7 cut(s)	128, 148, 634, 1094, 1294, 1418, 1625
Hin6I	GCGC	2 cut(s)	267, 887
HinP1I	GCGC	2 cut(s)	267, 887
HincII	GTYRAC	2 cut(s)	793, 1273
HindII	GTYRAC	2 cut(s)	793, 1273
HindIII	AAGCTT	2 cut(s)	296, 977
HinfI	GANTC	8 cut(s)	15, 69, 149, 362, 927, 1176, 1554, 1626
HpaI	GTTAAC	1 cut(s)	1273
HpaII	CCGG	3 cut(s)	32, 270, 1551
HphI	GGTGA	8 cut(s)	29, 619, 704, 842, 980, 1040, 1087, 1447
Hpy166II	GTNNAC	2 cut(s)	793, 1273
Hpy188I	TCNGA	1 cut(s)	68
Hpy8I	GTNNAC	2 cut(s)	793, 1273
Hpy99I	CGWCG	1 cut(s)	1403
HpyAV	CCTTC	8 cut(s)	46, 52, 154, 335, 1108, 1198, 1286, 1375
HpyCH4III	ACNGT	6 cut(s)	524, 800, 866, 1049, 1078, 1609
HpyCH4IV	ACGT	4 cut(s)	737, 1188, 1267, 1398
HpyF3I	CTNAG	5 cut(s)	10, 42, 294, 1244, 1425
HpySE526I	ACGT	4 cut(s)	737, 1188, 1267, 1398
Hsp92I	GRCGYC	1 cut(s)	267
Hsp92II	CATG	7 cut(s)	128, 148, 634, 1094, 1294, 1418, 1625
HspAI	GCGC	2 cut(s)	267, 887
KasI	GGCGCC	1 cut(s)	266
Ksp22I	TGATCA	2 cut(s)	430, 1411
KspAI	GTTAAC	1 cut(s)	1273
Kzo9I	GATC	7 cut(s)	34, 430, 480, 634, 916, 1411, 1570
LmnI	GCTCC	5 cut(s)	6, 172, 455, 703, 1639
Lsp1109I	GCAGC	3 cut(s)	295, 1327, 1588
LweI	GCATC	8 cut(s)	129, 295, 394, 580, 667, 823, 1225, 1360
MaeI	CTAG	5 cut(s)	459, 1230, 1578, 1638, 1678
MaeII	ACGT	4 cut(s)	737, 1188, 1267, 1398
MaeIII	GTNAC	6 cut(s)	251, 625, 841, 1093, 1402, 1418
MalI	GATC	7 cut(s)	36, 432, 482, 636, 918, 1413, 1572
MboI	GATC	7 cut(s)	34, 430, 480, 634, 916, 1411, 1570
MboII	GAAGA	4 cut(s)	1093, 1450, 1454, 1565
MfeI	CAATTG	4 cut(s)	434, 1323, 1521, 1527
MflI	RGATCY	2 cut(s)	480, 1570
MhlI	GDGCHC	4 cut(s)	11, 169, 196, 1302
MlsI	TGGCCA	2 cut(s)	1089, 1592
MluNI	TGGCCA	2 cut(s)	1089, 1592
Mly113I	GGCGCC	1 cut(s)	267
MlyI	GAGTC	3 cut(s)	63, 936, 1563
Mox20I	TGGCCA	2 cut(s)	1089, 1592
MroXI	GAANNNNTTC	1 cut(s)	329
MscI	TGGCCA	2 cut(s)	1089, 1592
MseI	TTAA	8 cut(s)	348, 357, 398, 450, 711, 1080, 1131, 1272
Msp20I	TGGCCA	2 cut(s)	1089, 1592
MspI	CCGG	3 cut(s)	32, 270, 1551
MspR9I	CCNGG	5 cut(s)	271, 287, 1539, 1551, 1631
MunI	CAATTG	4 cut(s)	434, 1323, 1521, 1527
Mva1269I	GAATGC	1 cut(s)	721
MvaI	CCWGG	3 cut(s)	287, 1539, 1631
NarI	GGCGCC	1 cut(s)	267
NciI	CCSGG	2 cut(s)	271, 1551
NcoI	CCATGG	1 cut(s)	630
NdeII	GATC	7 cut(s)	34, 430, 480, 634, 916, 1411, 1570
NheI	GCTAGC	1 cut(s)	458
NlaIII	CATG	7 cut(s)	128, 148, 634, 1094, 1294, 1418, 1625
NlaIV	GGNNCC	3 cut(s)	268, 750, 1635
NmeAIII	GCCGAG	3 cut(s)	157, 243, 403
NmuCI	GTSAC	4 cut(s)	251, 625, 1093, 1402
PasI	CCCWGGG	1 cut(s)	286
PcsI	WCGNNNNNNNCGW	2 cut(s)	72, 204
PctI	GAATGC	1 cut(s)	721
PdmI	GAANNNNTTC	1 cut(s)	329
PfeI	GAWTC	5 cut(s)	15, 149, 362, 1176, 1626
PfoI	TCCNGGA	1 cut(s)	1537
PkrI	GCNGC	4 cut(s)	310, 1342, 1583, 1603
PleI	GAGTC	3 cut(s)	63, 935, 1562
PluTI	GGCGCC	1 cut(s)	270
PpsI	GAGTC	3 cut(s)	63, 935, 1562
Ppu21I	YACGTR	1 cut(s)	1268
PpuMI	RGGWCCY	1 cut(s)	1535
PshAI	GACNNNNGTC	1 cut(s)	140
Psp124BI	GAGCTC	2 cut(s)	11, 196
Psp5II	RGGWCCY	1 cut(s)	1535
Psp6I	CCWGG	3 cut(s)	285, 1537, 1629
PspGI	CCWGG	3 cut(s)	285, 1537, 1629
PspN4I	GGNNCC	3 cut(s)	268, 750, 1635
PspPI	GGNCC	3 cut(s)	815, 862, 1535
PspPPI	RGGWCCY	1 cut(s)	1535
PstI	CTGCAG	1 cut(s)	1070
PsuI	RGATCY	2 cut(s)	480, 1570
RsaI	GTAC	2 cut(s)	113, 1266
RsaNI	GTAC	2 cut(s)	112, 1265
Rsr2I	CGGWCCG	1 cut(s)	862
RsrII	CGGWCCG	1 cut(s)	862
SacI	GAGCTC	2 cut(s)	11, 196
SaqAI	TTAA	8 cut(s)	348, 357, 398, 450, 711, 1080, 1131, 1272
SatI	GCNGC	4 cut(s)	309, 1341, 1582, 1602
Sau3AI	GATC	7 cut(s)	34, 430, 480, 634, 916, 1411, 1570
Sau96I	GGNCC	3 cut(s)	815, 862, 1535
SchI	GAGTC	3 cut(s)	63, 936, 1563
ScrFI	CCNGG	5 cut(s)	271, 287, 1539, 1551, 1631
SduI	GDGCHC	4 cut(s)	11, 169, 196, 1302
SfaNI	GCATC	8 cut(s)	129, 295, 394, 580, 667, 823, 1225, 1360
SfcI	CTRYAG	2 cut(s)	1066, 1508
SfoI	GGCGCC	1 cut(s)	268
SinI	GGWCC	2 cut(s)	862, 1535
SmlI	CTYRAG	2 cut(s)	155, 890
SmoI	CTYRAG	2 cut(s)	155, 890
SsiI	CCGC	6 cut(s)	746, 860, 1054, 1564, 1581, 1584
SspDI	GGCGCC	1 cut(s)	266
SspMI	CTAG	5 cut(s)	459, 1230, 1578, 1638, 1678
SstI	GAGCTC	2 cut(s)	11, 196
StyD4I	CCNGG	5 cut(s)	269, 285, 1537, 1549, 1629
StyI	CCWWGG	2 cut(s)	630, 882
TaaI	ACNGT	6 cut(s)	524, 800, 866, 1049, 1078, 1609
TaiI	ACGT	4 cut(s)	740, 1191, 1270, 1401
TaqI	TCGA	8 cut(s)	18, 333, 919, 925, 1212, 1377, 1467, 1662
TatI	WGTACW	1 cut(s)	111
TauI	GCSGC	1 cut(s)	1584
TfiI	GAWTC	5 cut(s)	15, 149, 362, 1176, 1626
Tru1I	TTAA	8 cut(s)	348, 357, 398, 450, 711, 1080, 1131, 1272
Tru9I	TTAA	8 cut(s)	348, 357, 398, 450, 711, 1080, 1131, 1272
TscAI	CASTG	4 cut(s)	505, 805, 1075, 1411
TseFI	GTSAC	4 cut(s)	251, 625, 1093, 1402
TseI	GCWGC	3 cut(s)	308, 1340, 1601
Tsp45I	GTSAC	4 cut(s)	251, 625, 1093, 1402
TspDTI	ATGAA	5 cut(s)	407, 479, 1217, 1362, 1628
TspGWI	ACGGA	5 cut(s)	213, 473, 845, 929, 1547
TspRI	CASTG	4 cut(s)	505, 805, 1075, 1411
VpaK11BI	GGWCC	2 cut(s)	862, 1535
XapI	RAATTY	5 cut(s)	327, 707, 1158, 1392, 1488
XmnI	GAANNNNTTC	1 cut(s)	329
XspI	CTAG	5 cut(s)	459, 1230, 1578, 1638, 1678

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.