Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0001333

Physical Location & Seq

Genomic Coordinates Reverse (-)

45113414 .. 45118276

Gene Structure 4863 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1324400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2763 bp

ATGGCGATCTCTCAGGAGCTATACCCATCGCAAGACGACCTGCTCTACGAAGAGGAGCTTCTCCGCAACCCATTCAGCCTCAAGCTATGGTGGCGCTACCTAATCGCTCGAGCCGAATCTCCGTTCAAGAAGCGCTTCATAATCTACGAGCGAGCCCTCAAGGCTCTGCCCGGAAGCTACAAGCTCTGGTACGCTTACCTCCGCGAAAGACTCGAGCTTGTAAGGAACTTGCCCATCACTCACTCGCAGTATGAAACACTAGTCAACACGTTTGAACGAGCCCTAGTGACGATGCACAAGATGCCCAGGATTTGGATTATGTACTTGCAGACTTTGACGGAGCAGAAATTGGTCACCAAGACTCGCCGGACCTTTGACAGAGCGCTGTGTGCTCTTCCCGTGACGCAGCACGATCGCATTTGGGAGCCCTACCTCGTGTTCGTGAGCCAAAAGGGCATCCCAATCGAGACCTCGCTTCGGGTTTATCGCAGGTATTTGAAATATGACCCTACCCATATTGAAGATTTCATCGAGTTCTTGATTAATTCTAGTCTTTGGCAAGAGGCTGCCGAGAGGCTGGCTGCTGTATTGAATGATGATAAGTTTTACTCGATAAAGGGGAAGACGAAACATAGGCTGTGGCTAGAATTGTGTGATTTGCTTACTAAGCATGCCACCGAGGTTTCGGGCCTCAATGTGGATGCTATAATTAGAGGTGGGATTAGGAAGTTTACAGATGAGGTGGGGCGGTTGTGGACCTCTCTTGCGGACTATTACATTAGAAGGAATTTGCATGAGAAGGCGAGGGATATATTCGAGGAGGGTATGACAACTGTGGTAACGGTGAGAGATTTTAGTGTGATTTTTGATTCGTATGCTCATTTTGAAGAGATTGTGCTTGCCCATAAGATGGAAACTGCTGATTTGAGTGATGATGAGGAGGACAAAGAGAATGGGGTTGCGGAGGATGGAAATGAGGAGGAAGAAGATCTTCGATTGGATATTAACTTGTCTGTGGCTGAGCTTGAGAAGAAAATGCTTGATGGGTTTTGGTTACATGATGATAAGGATATAGATTTGAGATTAGCTCGATTGGACCATCTCATGGATAGAAGACCTGTACTAGCAAATAGTGTTCTTTTACGACAAAATCCTCATAATGTAGAGCAGTGGCACCGAAGAGTGAAGCTTTATGAGGGCAATCCAACCAAGCAGATACTTACTTACACTGAGGCAGTGAGGACAATTGATCCAATGAAAGCGGTTGGGAAGCCTCACACTTTGTGGGTTGCTTTTGCTAAGTTGTATGAGAACCACAACGATATTGTCAATGCCAGAGTGATTTTTGATAAAGCAGTGCAGGTGAATTACAAGACCGTGGATAATTTGGCTAGTCTGTGGTGTGAGTGGGCAGAAATGGAATTGAGGCATAAGAATTTCAAAGGAGCACTGGAACTGATGAGGCGGGCTACTGCAGAGCCATCTGTTGAGGTGAAACGAAGAGTGGCTGCTGATGGGAATGCACCGGTTCAGATGAAGCTGCATAAATGCTTGAGGATTTGGGCCTTTTATGTGGACTTGGAGGAGAGCCTGGGTAAATTAGAGTCCACTCGAGCTGTTTACGAGAGGATATTGGATTTGAAAATAGCCACACCGCAGATCATAATCAATTATGCATCACTTCTTGAGGAGCATAAATACTTTGAAGATGCGTTCAAGGTGTATCAAAAAGGTACTAAAATATTCAAGTATCCACATGTCAAAGACATATGGGTTACATATCTCTCTAGATTTGTAAAGAGATATGGGAAGAAAGAACTTGAGCGCGCAAGAGAGCTATTTGAGGAGGCTGTTCAAGCGGCCCCTGCTGATGCGAAGAAGCCTTTGTATCTTCAATATGCAAAGCTGGAGGAGGATTATGGCCTATCAAAGCGGGCGATGAAAGTCTATGATGAAGCAACGAAGGCTGTTCCAAACCATGAGAAACTGGGCATGTATGAAATCTATATTGCCCGTGCCACAGAGATATTTGGCGTCCCAAAAACAAGGGATATATACCAGCAGGCCATAGATTCTGGTCTTCCAGACAAAGATGTGAAGACAATGTGTTTAAAGTTTGCTGAGCTTGAGAAGAGCTTGGGAGAAATTGATCGTGCTCGGGGAGTGTATACATTTGCGTCACAGTTTTCGGATCCACGATCTGATGTGGATTTCTGGAACAAATGGCATGAGTTTGAAGTGCAGCACGGAAATGAAGATACCTTCCGGGAGATGCTTCGAATTAAACGAAGTGTTTCCGCAAGCTATAGCCAGACGCACTTTATTCTACCCGAGTATATGATGCAAAAGGATCAGAGGCTGAACATGGACGAGGCAAAAGACAAGTTGAAACAGGCAGGAGTCCCCGAAGATGAAATGGCTGCTCTAGAGAGGCAGTTGGCACCTGTGGCCAACGATACTACCACTAAAGATAGCAGTAGAATAGGCTTTGTGAGTGCTGGAGCAATTCAACAGACCGACGGAGGGATCAAAGTTACCGCAAATCCTGAAGACATTGAGCTACCGGAAGAAAATGATTCAGAAGACGACGAAAGGGTGGAGATTCAAATTGCACAGAAGGAGGTCCCGGATGCTGTTTTTGGAGAGTTGGCTAACAAGAGAAAGGAGGCCGAAAAAGACGAGGGCGGAGATGCTGATACCAAGGACGATGGCAGCCGCCTTGGTGCCCTTGAGAGAATAAAAAGGCTAAAACGAGGCGCCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000349 GO:0000375 GO:0000377 GO:0000393 GO:0000398 GO:0000974 GO:0001701 GO:0001824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005681 GO:0005684 GO:0006139 GO:0006259 GO:0006281 GO:0006283 GO:0006289 GO:0006351 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007275 GO:0008150 GO:0008152 GO:0008380 GO:0009058 GO:0009059 GO:0009790 GO:0009792 GO:0009987 GO:0010467 GO:0016043 GO:0016070 GO:0016071 GO:0018130 GO:0019438 GO:0022607 GO:0022613 GO:0022618 GO:0032501 GO:0032502 GO:0032774 GO:0032991 GO:0033554 GO:0034622 GO:0034641 GO:0034645 GO:0034654 GO:0043009 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0048856 GO:0050896 GO:0051716 GO:0065003 GO:0071007 GO:0071010 GO:0071012 GO:0071013 GO:0071014 GO:0071704 GO:0071826 GO:0071840 GO:0090304 GO:0097659 GO:1901360 GO:1901362 GO:1901576 GO:1902494 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko03040 map03040

Pfam Domains

Protein Families

Suf

Protein Analysis

921

Amino Acids

107.21

Weight (kDa)

5.83

Isoelectric Point (pI)

44.83

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HAT_Syf1_CNRKL1_N	PF23233	12 - 170	1.4e-60	Pre-mRNA-splicing factor Syf1/CNRKL1 N-terminal HAT repeat
HAT_PRP39_N	PF23240	15 - 143	1.3e-07	PRP39 N-terminal HAT repeat
HAT_Syf1_M	PF23220	173 - 335	1.8e-58	Pre-mRNA-splicing factor SYF1 middle HAT repeat
HAT_Syf1_M	PF23220	346 - 416	2.6e-24	Pre-mRNA-splicing factor SYF1 middle HAT repeat
HAT_Syf1_CNRKL1_C	PF23231	418 - 812	1.6e-190	Pre-mRNA-splicing factor Syf1/CRNKL1 C-terminal HAT repeat
Suf	PF05843	420 - 663	4.9e-07	Suppressor of forked protein (Suf)
HAT_PRP39_C	PF23241	517 - 583	2e-06	PRP39 C-terminal HAT repeat
HAT_PRP39_N	PF23240	557 - 655	2e-08	PRP39 N-terminal HAT repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0001333)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G28740
fragaria_vesca	FvH4_3g03910 FvH4_3g04150 FvH4_3g04150 FvH4_3g04150 FvH4_3g04150
malus_domestica	MD05G1324400.v1.1 MD10G1302200.v1.1
prunus_persica	Prupe.5G013200_v2.0.a1
pyrus_communis	pycom05g29860 pycom10g25470
rosa_chinensis	RchiOBHm_Chr5g0006181 RchiOBHm_Chr5g0006191 RchiOBHm_Chr5g0006201 RchiOBHm_Chr5g0006601 RchiOBHm_Chr7g0193971
rosa_laevigata	RLG00000004227 RLG00000031375 RLG00000031376 RLG00000031403
rosa_multiflora	Rmu_co7988368.1_g000001 Rmu_co8257165.1_g000001 Rmu_co8304153.1_g000001 Rmu_co8327759.1_g000001 Rmu_sc0001972.1_g000002 Rmu_sc0002389.1_g000006 Rmu_sc0004331.1_g000002 Rmu_sc0005583.1_g000001 Rmu_sc0009371.1_g000007 Rmu_sc0011154.1_g000005 Rmu_sc0024345.1_g000002 Rmu_sc0032392.1_g000001 Rmu_sc0032393.1_g000001
rosa_roxburghii	Rroxscaffold_1G00069420 Rroxscaffold_1G00069440 Rroxscaffold_1G00069800 Rroxscaffold_2G00122720 Rroxscaffold_3G00261670
rosa_rugosa	Rorug04G0422900
rosa_samantha	Rh5AG050200 Rh5BG046700 Rh5BG046800 Rh5BG049400 Rh5CG055400 Rh5CG055600 Rh5CG057800 Rh5DG046500 Rh5DG048800 Rh7AG139900 Rh7AG140000 Rh7DG141300
rosa_wichuraiana	Rw5G004790 Rw5G005020 Rw7G011940

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1351
Acc36I	ACCTGC	3 cut(s)	48, 480, 1351
AccB1I	GGYRCC	4 cut(s)	1173, 2440, 2723, 2756
AccB7I	CCANNNNNTGG	3 cut(s)	312, 910, 1105
AccI	GTMKAC	1 cut(s)	2168
AccII	CGCG	2 cut(s)	204, 1827
AclWI	GGATC	5 cut(s)	1244, 2186, 2199, 2358, 2534
AcoI	YGGCCR	1 cut(s)	2448
AcsI	RAATTY	2 cut(s)	787, 1435
AcuI	CTGAAG	1 cut(s)	2568
AcyI	GRCGYC	2 cut(s)	2034, 2757
AdeI	CACNNNGTG	1 cut(s)	1284
AfaI	GTAC	4 cut(s)	191, 323, 1122, 1735
AfeI	AGCGCT	2 cut(s)	134, 384
AfiI	CCNNNNNNNGG	6 cut(s)	312, 477, 697, 910, 1105, 2523
AflIII	ACRYGT	2 cut(s)	267, 1756
AgeI	ACCGGT	1 cut(s)	1525
AhlI	ACTAGT	1 cut(s)	259
AjnI	CCWGG	2 cut(s)	305, 1590
Alw21I	GWGCWC	3 cut(s)	394, 1450, 2158
Alw26I	GTCTC	1 cut(s)	461
AlwI	GGATC	5 cut(s)	1244, 2186, 2199, 2358, 2534
AlwNI	CAGNNNCTG	1 cut(s)	2359
Ama87I	CYCGRG	5 cut(s)	108, 212, 1611, 2157, 2330
Aor51HI	AGCGCT	2 cut(s)	134, 384
AoxI	GGCC	7 cut(s)	688, 1563, 1860, 1921, 2064, 2448, 2667
ApeKI	GCWGC	8 cut(s)	406, 566, 581, 1508, 1540, 2242, 2420, 2712
ApoI	RAATTY	2 cut(s)	787, 1435
AseI	ATTAAT	1 cut(s)	543
AsiGI	ACCGGT	1 cut(s)	1525
Asp700I	GAANNNNTTC	3 cut(s)	134, 990, 2293
AspLEI	GCGC	6 cut(s)	96, 135, 385, 1827, 1829, 2759
AspS9I	GGNCC	7 cut(s)	369, 688, 756, 1096, 1563, 1861, 2623
AsuC2I	CCSGG	3 cut(s)	171, 2267, 2627
AsuHPI	GGTGA	4 cut(s)	346, 856, 1375, 1504
AsuII	TTCGAA	1 cut(s)	2278
AvaI	CYCGRG	5 cut(s)	108, 212, 1611, 2157, 2330
AvaII	GGWCC	4 cut(s)	369, 756, 1096, 2623
BaeGI	GKGCMC	1 cut(s)	2728
BalI	TGGCCA	1 cut(s)	2450
BamHI	GGATCC	1 cut(s)	2191
BanI	GGYRCC	4 cut(s)	1173, 2440, 2723, 2756
BanII	GRGCYC	3 cut(s)	157, 283, 429
BauI	CACGAG	1 cut(s)	434
BbsI	GAAGAC	6 cut(s)	629, 1120, 2072, 2105, 2556, 2589
Bbv12I	GWGCWC	3 cut(s)	394, 1450, 2158
BbvI	GCAGC	8 cut(s)	418, 553, 568, 1495, 1527, 2254, 2407, 2724
BccI	CCATC	9 cut(s)	34, 242, 904, 962, 1037, 1107, 1489, 1508, 2702
BcgI	CGANNNNNNTGC	4 cut(s)	395, 429, 445, 479
BciT130I	CCWGG	2 cut(s)	307, 1592
BciVI	GTATCC	1 cut(s)	1761
BcnI	CCSGG	3 cut(s)	171, 2267, 2627
BcoDI	GTCTC	1 cut(s)	461
BcuI	ACTAGT	1 cut(s)	259
BfaI	CTAG	8 cut(s)	260, 284, 549, 644, 1124, 1392, 1788, 2426
BfmI	CTRYAG	2 cut(s)	1473, 2305
BfoI	RGCGCY	4 cut(s)	97, 136, 386, 2760
BfuAI	ACCTGC	3 cut(s)	48, 480, 1351
BfuI	GTATCC	1 cut(s)	1761
BglI	GCCNNNNNGGC	2 cut(s)	161, 453
BglII	AGATCT	1 cut(s)	988
BlpI	GCTNAGC	2 cut(s)	1020, 2121
Bme1390I	CCNGG	5 cut(s)	171, 307, 1592, 2267, 2627
Bme18I	GGWCC	4 cut(s)	369, 756, 1096, 2623
BmeT110I	CYCGRG	5 cut(s)	108, 212, 1611, 2157, 2330
BmgT120I	GGNCC	7 cut(s)	369, 688, 756, 1096, 1563, 1861, 2623
BmiI	GGNNCC	8 cut(s)	426, 1175, 1863, 2193, 2442, 2625, 2725, 2758
BmrFI	CCNGG	5 cut(s)	171, 307, 1592, 2267, 2627
BmrI	ACTGGG	1 cut(s)	1997
BmuI	ACTGGG	1 cut(s)	1997
BpiI	GAAGAC	6 cut(s)	629, 1120, 2072, 2105, 2556, 2589
BplI	GAGNNNNNCTC	2 cut(s)	1072, 1104
BpmI	CTGGAG	2 cut(s)	1928, 2520
Bpu1102I	GCTNAGC	2 cut(s)	1020, 2121
Bpu14I	TTCGAA	1 cut(s)	2278
BpuEI	CTTGAG	8 cut(s)	65, 143, 1046, 1573, 1706, 1841, 2147, 2750
BpuMI	CCSGG	3 cut(s)	171, 2267, 2627
BsaBI	GATNNNNATC	1 cut(s)	1664
BsaHI	GRCGYC	2 cut(s)	2034, 2757
BsaI	GGTCTC	1 cut(s)	461
BsaJI	CCNNGG	6 cut(s)	305, 678, 1377, 1591, 2700, 2719
BsaWI	WCCGGW	2 cut(s)	1525, 2563
Bsc4I	CCNNNNNNNGG	6 cut(s)	312, 477, 697, 910, 1105, 2523
Bse118I	RCCGGY	1 cut(s)	1525
Bse1I	ACTGG	2 cut(s)	1455, 1992
Bse8I	GATNNNNATC	1 cut(s)	1664
BseBI	CCWGG	2 cut(s)	307, 1592
BseDI	CCNNGG	6 cut(s)	305, 678, 1377, 1591, 2700, 2719
BseGI	GGATG	4 cut(s)	456, 706, 973, 2635
BseJI	GATNNNNATC	1 cut(s)	1664
BseLI	CCNNNNNNNGG	6 cut(s)	312, 477, 697, 910, 1105, 2523
BseMII	CTCAG	4 cut(s)	26, 1011, 1221, 2112
BseNI	ACTGG	2 cut(s)	1455, 1992
BsePI	GCGCGC	1 cut(s)	1825
BseRI	GAGGAG	8 cut(s)	68, 833, 953, 992, 1598, 1703, 1859, 1925
BseSI	GKGCMC	1 cut(s)	2728
BseXI	GCAGC	8 cut(s)	418, 553, 568, 1495, 1527, 2254, 2407, 2724
BsgI	GTGCAG	2 cut(s)	1379, 2261
Bsh1236I	CGCG	2 cut(s)	204, 1827
Bsh1285I	CGRYCG	1 cut(s)	415
BshFI	GGCC	7 cut(s)	690, 1565, 1862, 1923, 2066, 2450, 2669
BshNI	GGYRCC	4 cut(s)	1173, 2440, 2723, 2756
BshTI	ACCGGT	1 cut(s)	1525
BsiEI	CGRYCG	1 cut(s)	415
BsiHKAI	GWGCWC	3 cut(s)	394, 1450, 2158
BsiHKCI	CYCGRG	5 cut(s)	108, 212, 1611, 2157, 2330
BsiSI	CCGG	6 cut(s)	171, 367, 1526, 2266, 2564, 2627
BslFI	GGGAC	3 cut(s)	2021, 2387, 2609
BslI	CCNNNNNNNGG	6 cut(s)	312, 477, 697, 910, 1105, 2523
BsmAI	GTCTC	1 cut(s)	461
BsmFI	GGGAC	3 cut(s)	2021, 2387, 2609
BsmI	GAATGC	1 cut(s)	1525
BsnI	GGCC	7 cut(s)	690, 1565, 1862, 1923, 2066, 2450, 2669
Bso31I	GGTCTC	1 cut(s)	461
BsoBI	CYCGRG	5 cut(s)	108, 212, 1611, 2157, 2330
Bsp119I	TTCGAA	1 cut(s)	2278
Bsp1286I	GDGCHC	7 cut(s)	157, 283, 394, 429, 1450, 2158, 2728
Bsp1720I	GCTNAGC	2 cut(s)	1020, 2121
BspANI	GGCC	7 cut(s)	690, 1565, 1862, 1923, 2066, 2450, 2669
BspCNI	CTCAG	4 cut(s)	25, 1012, 1222, 2113
BspFNI	CGCG	2 cut(s)	204, 1827
BspLI	GGNNCC	8 cut(s)	426, 1175, 1863, 2193, 2442, 2625, 2725, 2758
BspMAI	CTGCAG	1 cut(s)	1477
BspMI	ACCTGC	3 cut(s)	48, 480, 1351
BspPI	GGATC	5 cut(s)	1244, 2186, 2199, 2358, 2534
BspQI	GCTCTTC	2 cut(s)	399, 2126
BspT104I	TTCGAA	1 cut(s)	2278
BspT107I	GGYRCC	4 cut(s)	1173, 2440, 2723, 2756
BspTNI	GGTCTC	1 cut(s)	461
BsrFI	RCCGGY	1 cut(s)	1525
BsrI	ACTGG	2 cut(s)	1455, 1992
BssAI	RCCGGY	1 cut(s)	1525
BssECI	CCNNGG	6 cut(s)	305, 678, 1377, 1591, 2700, 2719
BssHII	GCGCGC	1 cut(s)	1825
BssNAI	GTATAC	1 cut(s)	2169
BssNI	GRCGYC	2 cut(s)	2034, 2757
BssSI	CACGAG	1 cut(s)	434
BssT1I	CCWWGG	2 cut(s)	2700, 2719
Bst1107I	GTATAC	1 cut(s)	2169
Bst2BI	CACGAG	1 cut(s)	434
Bst2UI	CCWGG	2 cut(s)	307, 1592
Bst4CI	ACNGT	4 cut(s)	835, 844, 1378, 2184
Bst6I	CTCTTC	6 cut(s)	45, 399, 882, 1174, 1495, 2126
BstACI	GRCGYC	2 cut(s)	2034, 2757
BstAPI	GCANNNNNTGC	1 cut(s)	301
BstBI	TTCGAA	1 cut(s)	2278
BstC8I	GCNNGC	9 cut(s)	153, 579, 672, 900, 1467, 1827, 1935, 2064, 2302
BstDEI	CTNAG	6 cut(s)	12, 666, 1020, 1230, 1299, 2121
BstDSI	CCRYGG	1 cut(s)	1377
BstEII	GGTNACC	1 cut(s)	352
BstF5I	GGATG	4 cut(s)	456, 706, 973, 2635
BstFNI	CGCG	2 cut(s)	204, 1827
BstH2I	RGCGCY	4 cut(s)	97, 136, 386, 2760
BstHHI	GCGC	6 cut(s)	96, 135, 385, 1827, 1829, 2759
BstMAI	GTCTC	1 cut(s)	461
BstMCI	CGRYCG	1 cut(s)	415
BstNI	CCWGG	2 cut(s)	307, 1592
BstNSI	RCATGY	3 cut(s)	674, 1760, 1996
BstPI	GGTNACC	1 cut(s)	352
BstSCI	CCNGG	5 cut(s)	169, 305, 1590, 2265, 2625
BstSFI	CTRYAG	2 cut(s)	1473, 2305
BstSLI	GKGCMC	1 cut(s)	2728
BstUI	CGCG	2 cut(s)	204, 1827
BstV1I	GCAGC	8 cut(s)	418, 553, 568, 1495, 1527, 2254, 2407, 2724
BstV2I	GAAGAC	6 cut(s)	629, 1120, 2072, 2105, 2556, 2589
BstX2I	RGATCY	2 cut(s)	988, 2191
BstYI	RGATCY	2 cut(s)	988, 2191
BstZ17I	GTATAC	1 cut(s)	2169
BsuI	GTATCC	1 cut(s)	1761
BsuRI	GGCC	7 cut(s)	690, 1565, 1862, 1923, 2066, 2450, 2669
BtgI	CCRYGG	1 cut(s)	1377
BtgZI	GCGATG	2 cut(s)	12, 1952
BtsCI	GGATG	4 cut(s)	456, 706, 973, 2635
BtsI	GCAGTG	3 cut(s)	1175, 1242, 1362
BtsIMutI	CAGTG	5 cut(s)	1175, 1227, 1242, 1362, 1448
BveI	ACCTGC	3 cut(s)	48, 480, 1351
Cac8I	GCNNGC	9 cut(s)	153, 579, 672, 900, 1467, 1827, 1935, 2064, 2302
CaiI	CAGNNNCTG	1 cut(s)	2359
CfoI	GCGC	6 cut(s)	96, 135, 385, 1827, 1829, 2759
Cfr10I	RCCGGY	1 cut(s)	1525
Cfr13I	GGNCC	7 cut(s)	369, 688, 756, 1096, 1563, 1861, 2623
CseI	GACGC	4 cut(s)	412, 2023, 2166, 2323
Csp6I	GTAC	4 cut(s)	190, 322, 1121, 1734
CspAI	ACCGGT	1 cut(s)	1525
CviAII	CATG	9 cut(s)	671, 794, 1058, 1105, 1757, 1979, 1993, 2228, 2365
CviQI	GTAC	4 cut(s)	190, 322, 1121, 1734
DdeI	CTNAG	6 cut(s)	12, 666, 1020, 1230, 1299, 2121
DinI	GGCGCC	1 cut(s)	2758
DraI	TTTAAA	1 cut(s)	2112
DraIII	CACNNNGTG	1 cut(s)	1284
EaeI	YGGCCR	1 cut(s)	2448
Eam1104I	CTCTTC	6 cut(s)	45, 399, 882, 1174, 1495, 2126
EarI	CTCTTC	6 cut(s)	45, 399, 882, 1174, 1495, 2126
EciI	GGCGGA	1 cut(s)	2700
Eco130I	CCWWGG	2 cut(s)	2700, 2719
Eco24I	GRGCYC	3 cut(s)	157, 283, 429
Eco31I	GGTCTC	1 cut(s)	461
Eco47I	GGWCC	4 cut(s)	369, 756, 1096, 2623
Eco47III	AGCGCT	2 cut(s)	134, 384
Eco57I	CTGAAG	1 cut(s)	2568
Eco88I	CYCGRG	5 cut(s)	108, 212, 1611, 2157, 2330
Eco91I	GGTNACC	1 cut(s)	352
EcoO109I	RGGNCCY	1 cut(s)	2623
EcoO65I	GGTNACC	1 cut(s)	352
EcoRII	CCWGG	2 cut(s)	305, 1590
EcoT14I	CCWWGG	2 cut(s)	2700, 2719
EcoT22I	ATGCAT	1 cut(s)	1678
EcoT38I	GRGCYC	3 cut(s)	157, 283, 429
EgeI	GGCGCC	1 cut(s)	2758
EheI	GGCGCC	1 cut(s)	2758
ErhI	CCWWGG	2 cut(s)	2700, 2719
FaeI	CATG	9 cut(s)	674, 797, 1061, 1108, 1760, 1982, 1996, 2231, 2368
FalI	AAGNNNNNCTT	6 cut(s)	42, 74, 119, 151, 975, 1007
FaqI	GGGAC	3 cut(s)	2021, 2387, 2609
FatI	CATG	9 cut(s)	670, 793, 1057, 1104, 1756, 1978, 1992, 2227, 2364
FauI	CCCGC	2 cut(s)	1458, 1926
FauNDI	CATATG	1 cut(s)	1769
FblI	GTMKAC	1 cut(s)	2168
FokI	GGATG	4 cut(s)	443, 713, 980, 2642
FriOI	GRGCYC	3 cut(s)	157, 283, 429
FspBI	CTAG	8 cut(s)	260, 284, 549, 644, 1124, 1392, 1788, 2426
GlaI	GCGC	6 cut(s)	95, 134, 384, 1826, 1828, 2758
GsuI	CTGGAG	2 cut(s)	1928, 2520
HaeII	RGCGCY	4 cut(s)	97, 136, 386, 2760
HaeIII	GGCC	7 cut(s)	690, 1565, 1862, 1923, 2066, 2450, 2669
HapII	CCGG	6 cut(s)	171, 367, 1526, 2266, 2564, 2627
HgaI	GACGC	4 cut(s)	412, 2023, 2166, 2323
HhaI	GCGC	6 cut(s)	96, 135, 385, 1827, 1829, 2759
Hin1I	GRCGYC	2 cut(s)	2034, 2757
Hin1II	CATG	9 cut(s)	674, 797, 1061, 1108, 1760, 1982, 1996, 2231, 2368
Hin6I	GCGC	6 cut(s)	94, 133, 383, 1825, 1827, 2757
HinP1I	GCGC	6 cut(s)	94, 133, 383, 1825, 1827, 2757
HincII	GTYRAC	1 cut(s)	265
HindII	GTYRAC	1 cut(s)	265
HindIII	AAGCTT	1 cut(s)	1187
HinfI	GANTC	9 cut(s)	116, 210, 361, 869, 1604, 2072, 2400, 2576, 2602
HpaII	CCGG	6 cut(s)	171, 367, 1526, 2266, 2564, 2627
HphI	GGTGA	4 cut(s)	346, 856, 1375, 1504
Hpy166II	GTNNAC	7 cut(s)	265, 732, 756, 1576, 1608, 1621, 2169
Hpy188I	TCNGA	5 cut(s)	1533, 2191, 2203, 2355, 2581
Hpy8I	GTNNAC	7 cut(s)	265, 732, 756, 1576, 1608, 1621, 2169
Hpy99I	CGWCG	2 cut(s)	2522, 2591
HpyAV	CCTTC	5 cut(s)	777, 793, 1957, 2272, 2611
HpyCH4III	ACNGT	4 cut(s)	835, 844, 1378, 2184
HpyCH4IV	ACGT	1 cut(s)	269
HpyF3I	CTNAG	6 cut(s)	12, 666, 1020, 1230, 1299, 2121
HpySE526I	ACGT	1 cut(s)	269
Hsp92I	GRCGYC	2 cut(s)	2034, 2757
Hsp92II	CATG	9 cut(s)	674, 797, 1061, 1108, 1760, 1982, 1996, 2231, 2368
HspAI	GCGC	6 cut(s)	94, 133, 383, 1825, 1827, 2757
KasI	GGCGCC	1 cut(s)	2756
LguI	GCTCTTC	2 cut(s)	399, 2126
LmnI	GCTCC	7 cut(s)	16, 55, 340, 424, 1445, 1690, 2501
Lsp1109I	GCAGC	8 cut(s)	418, 553, 568, 1495, 1527, 2254, 2407, 2724
MaeI	CTAG	8 cut(s)	260, 284, 549, 644, 1124, 1392, 1788, 2426
MaeII	ACGT	1 cut(s)	269
MaeIII	GTNAC	8 cut(s)	286, 352, 400, 838, 1053, 1774, 2178, 2533
MfeI	CAATTG	1 cut(s)	1245
MflI	RGATCY	2 cut(s)	988, 2191
MhlI	GDGCHC	7 cut(s)	157, 283, 394, 429, 1450, 2158, 2728
MlsI	TGGCCA	1 cut(s)	2450
MluNI	TGGCCA	1 cut(s)	2450
Mly113I	GGCGCC	1 cut(s)	2757
MlyI	GAGTC	4 cut(s)	204, 355, 1613, 2409
MmeI	TCCRAC	1 cut(s)	1229
Mox20I	TGGCCA	1 cut(s)	2450
Mph1103I	ATGCAT	1 cut(s)	1678
MroXI	GAANNNNTTC	3 cut(s)	134, 990, 2293
MscI	TGGCCA	1 cut(s)	2450
MseI	TTAA	4 cut(s)	543, 1005, 2111, 2283
MslI	CAYNNNNRTG	1 cut(s)	2250
Msp20I	TGGCCA	1 cut(s)	2450
MspI	CCGG	6 cut(s)	171, 367, 1526, 2266, 2564, 2627
MspR9I	CCNGG	5 cut(s)	171, 307, 1592, 2267, 2627
MunI	CAATTG	1 cut(s)	1245
Mva1269I	GAATGC	1 cut(s)	1525
MvaI	CCWGG	2 cut(s)	307, 1592
MvnI	CGCG	2 cut(s)	204, 1827
NarI	GGCGCC	1 cut(s)	2757
NciI	CCSGG	3 cut(s)	171, 2267, 2627
NdeI	CATATG	1 cut(s)	1769
NlaIII	CATG	9 cut(s)	674, 797, 1061, 1108, 1760, 1982, 1996, 2231, 2368
NlaIV	GGNNCC	8 cut(s)	426, 1175, 1863, 2193, 2442, 2625, 2725, 2758
NmeAIII	GCCGAG	1 cut(s)	595
NmuCI	GTSAC	4 cut(s)	286, 352, 400, 2178
NsiI	ATGCAT	1 cut(s)	1678
NspI	RCATGY	3 cut(s)	674, 1760, 1996
NspV	TTCGAA	1 cut(s)	2278
PaeI	GCATGC	1 cut(s)	674
PaeR7I	CTCGAG	3 cut(s)	108, 212, 1611
PaqCI	CACCTGC	1 cut(s)	1351
PauI	GCGCGC	1 cut(s)	1825
PciI	ACATGT	1 cut(s)	1756
PciSI	GCTCTTC	2 cut(s)	399, 2126
PcsI	WCGNNNNNNNCGW	2 cut(s)	111, 2284
PctI	GAATGC	1 cut(s)	1525
PdmI	GAANNNNTTC	3 cut(s)	134, 990, 2293
PfeI	GAWTC	5 cut(s)	116, 869, 2072, 2576, 2602
PflMI	CCANNNNNTGG	3 cut(s)	312, 910, 1105
PfoI	TCCNGGA	2 cut(s)	2265, 2625
PinAI	ACCGGT	1 cut(s)	1525
Ple19I	CGATCG	1 cut(s)	415
PleI	GAGTC	4 cut(s)	204, 355, 1612, 2408
PluTI	GGCGCC	1 cut(s)	2760
PpsI	GAGTC	4 cut(s)	204, 355, 1612, 2408
PpuMI	RGGWCCY	1 cut(s)	2623
PscI	ACATGT	1 cut(s)	1756
PshBI	ATTAAT	1 cut(s)	543
Psp5II	RGGWCCY	1 cut(s)	2623
Psp6I	CCWGG	2 cut(s)	305, 1590
PspEI	GGTNACC	1 cut(s)	352
PspGI	CCWGG	2 cut(s)	305, 1590
PspN4I	GGNNCC	8 cut(s)	426, 1175, 1863, 2193, 2442, 2625, 2725, 2758
PspPI	GGNCC	7 cut(s)	369, 688, 756, 1096, 1563, 1861, 2623
PspPPI	RGGWCCY	1 cut(s)	2623
PspXI	VCTCGAGB	3 cut(s)	108, 212, 1611
PstI	CTGCAG	1 cut(s)	1477
PstNI	CAGNNNCTG	1 cut(s)	2359
PsuI	RGATCY	2 cut(s)	988, 2191
PteI	GCGCGC	1 cut(s)	1825
PvuI	CGATCG	1 cut(s)	415
RsaI	GTAC	4 cut(s)	191, 323, 1122, 1735
RsaNI	GTAC	4 cut(s)	190, 322, 1121, 1734
RseI	CAYNNNNRTG	1 cut(s)	2250
SapI	GCTCTTC	2 cut(s)	399, 2126
SaqAI	TTAA	4 cut(s)	543, 1005, 2111, 2283
Sau96I	GGNCC	7 cut(s)	369, 688, 756, 1096, 1563, 1861, 2623
SchI	GAGTC	4 cut(s)	204, 355, 1613, 2409
ScrFI	CCNGG	5 cut(s)	171, 307, 1592, 2267, 2627
SduI	GDGCHC	7 cut(s)	157, 283, 394, 429, 1450, 2158, 2728
SfcI	CTRYAG	2 cut(s)	1473, 2305
SfoI	GGCGCC	1 cut(s)	2758
Sfr274I	CTCGAG	3 cut(s)	108, 212, 1611
SfuI	TTCGAA	1 cut(s)	2278
SinI	GGWCC	4 cut(s)	369, 756, 1096, 2623
SlaI	CTCGAG	3 cut(s)	108, 212, 1611
SmiMI	CAYNNNNRTG	1 cut(s)	2250
SpeI	ACTAGT	1 cut(s)	259
SphI	GCATGC	1 cut(s)	674
SspDI	GGCGCC	1 cut(s)	2756
SspI	AATATT	1 cut(s)	1743
SspMI	CTAG	8 cut(s)	260, 284, 549, 644, 1124, 1392, 1788, 2426
StyD4I	CCNGG	5 cut(s)	169, 305, 1590, 2265, 2625
StyI	CCWWGG	2 cut(s)	2700, 2719
TaaI	ACNGT	4 cut(s)	835, 844, 1378, 2184
TaiI	ACGT	1 cut(s)	272
TaqII	GACCGA	1 cut(s)	2531
TatI	WGTACW	2 cut(s)	321, 1120
TauI	GCSGC	2 cut(s)	1862, 2718
TfiI	GAWTC	5 cut(s)	116, 869, 2072, 2576, 2602
Tru1I	TTAA	4 cut(s)	543, 1005, 2111, 2283
Tru9I	TTAA	4 cut(s)	543, 1005, 2111, 2283
TscAI	CASTG	5 cut(s)	1175, 1234, 1242, 1362, 1455
TseFI	GTSAC	4 cut(s)	286, 352, 400, 2178
TseI	GCWGC	8 cut(s)	406, 566, 581, 1508, 1540, 2242, 2420, 2712
Tsp45I	GTSAC	4 cut(s)	286, 352, 400, 2178
TspGWI	ACGGA	4 cut(s)	111, 353, 2262, 2535
TspRI	CASTG	5 cut(s)	1175, 1234, 1242, 1362, 1455
Van91I	CCANNNNNTGG	3 cut(s)	312, 910, 1105
VpaK11BI	GGWCC	4 cut(s)	369, 756, 1096, 2623
VspI	ATTAAT	1 cut(s)	543
XapI	RAATTY	2 cut(s)	787, 1435
XbaI	TCTAGA	2 cut(s)	1787, 2425
XceI	RCATGY	3 cut(s)	674, 1760, 1996
XhoI	CTCGAG	3 cut(s)	108, 212, 1611
XmiI	GTMKAC	1 cut(s)	2168
XmnI	GAANNNNTTC	3 cut(s)	134, 990, 2293
XspI	CTAG	8 cut(s)	260, 284, 549, 644, 1124, 1392, 1788, 2426
Zsp2I	ATGCAT	1 cut(s)	1678

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.