Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr7

Orthogroup ID

OG0001333

Physical Location & Seq

Genomic Coordinates Forward (+)

4801569 .. 4806026

Gene Structure 4458 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000031403

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2769 bp

ATGGCTATCTCTCAGGAACTCTACCCCTCCCAAGATGACCTTCTCTACGAAGAAGAGCTTCTCCGAAACCCCTACAGCCTCAAGCTCTGGTGGCGCTACCTAATCGCCCGATCCGACGCGCCCTTCAAGAAGCGCCGCACCATCTACGAGCGCGCCGTCAAGGCCCTCCCCGGAAGCTACAAGCTCTGGTACGCCTACCTCCGCGAGCGCCTCGAGCTCGTCCGCAACTTTCCGATCAACCACTCCGAGTACGAAACCCTAAACAACACCTTCGAGAGGGCGTTGGTCACAATGCACAAGATGCCGCGGATTTGGATTCTGTATTTACAGAGCCTGACGGAGCAGAGATTGGTCACCAGGACTCGCCGGAGTTTTGACCGGGCGCTGTGTGCTCTTCCGGTGCAGCAGCACGACCGGATTTGGGAGCTGTATTTGGTGTTCGTGAGCCAGAAGGGGATACCTATCCAGACCTCGCTTCGGGTCTACCGGAGGTACTTGCTTTATGACCCGACTCATGTTGAGGATTTTATTGCGTTTTTGATAGAGTCTGAGCTGTGGCAAGAGGCGGCGGAGCGGCTGGCCTCGGTGCTTAATGATGATCAGTTTAGGTCAATTAAGGGGAAGACGAGGCATAGGCTGTGGTTGGAGTTGTGTGATTTGCTTACTAAGAATGCCACGGCAGTTTCGGGGTTGAATGTGGATGCTATCATTAGAGGTGGGATTAAGAAGTTTACGGATGAGGTGGGAAGGCTGTGGACGTCGTTGGCGGATTATTATATTAAGAGGAGTTTGTTTGAGAAGGCTAGGGATGTGTTTGAGGAGGGGATGCAGACTGTGGTGACTGTGAGGGATTTTAGTGTGATTTTCGATGCGTATGCGCAGTTTGAAGAGAGTATGCTTGCGATTAAGATGGAGGCTTTGGGAAGTGATGAGGAGGAGGAGGAGGAGGAGGAGGAGGAAGGAGAGAATGGGAGAATGGAAGATGAGAGAAGTGAAGAGGAGGAGGAAGATGTCCGGACCAATGTAGACTTGTCTGTGGCTGAGCTTGAGAGGAAAATACTCCATGGGTTTTGGCTACATGATGAGAATGATGTTGATTTGAGGTTGGCTAGATTGGACCATCTTATGGATAGAAGACCCGAACTGGCTAATAGTGTTCTTTTGAGACAAAATCCGCATAATGTAGAGCAATGGCACCGGAGAGTGAAGCTGTTTGAGGGGAATCCTACAAAGCAGATACTTACTTACACCCAGGCTGTGAAGACAGTGGATCCAATGCAAGCAGTGGGGAAGCCTCATACGTTGTGGGTTGCTTTTGCTAAGCTGTATGAAACACATGGAGATATTGCAAATGCAAGAGTAATTTTTGATAAGGCCGTCCAGGTGAACTACAAGACGGTGGATAATCTGGCTAGTCTATGGTGTGAGTGGGCAGAAATGGAATTGCGGCATAAGAATTTTAAACGAGCCCTGGAACTGATGAGTAGAGCTACAGCAGAGCCGTCTGTTGAGGTCAAACGAAGAGTTGCTGCTGATGGGAATCCACCAGTTCAGATGAGGCTCCATAAATCCTTGAGGCTTTGGACCTTTTATGTGGACTTGGAGGAGAGCCTGGGTACACTAGAGTCTACTCGGGCAGTATATGAGAGGATATTGGATTTGAGAATAGCCACACCTCAAATAATAATCAACTATGCATTGCTTCTGGAAGAACATAAATACTTTGAAGATGCATTCAAAGTCTATGAAAAGGGTACTCAAATATTCAAGTACCCGCATGTCAAAGACATATGGATTACATATCTCTCTAAATTTGTAAAAAGATATGGGAAGAATAAGCTCGAACGTGCAAGACTGCTCTTTGAGGATGCTGTTAAAGCGGCTCCTGCTGATGCGAAGAAACCTTTGTATCTTCAATTTGCAAAGCTGGAGGAGGATTATGGTCTAGCAAAGCGGGCTATGAAGGTCTATGATGAAGCAACTAAGGCTGTTCCAAACCATGAAAAGTTGAGCATGTATGAAATCTATATTGCCCGTGCAGCTGAGATATTTGGGATCCCCAAAACAAGGGAGATATATGAGCAAGCAATAGAATCTGGTCTCCCTGACAAAGATGTGAAGACAATGTGTCTAAAGTATGCTGAGCTCGAGAAGAGCTTGGGCGAAATTGATCGTGCTCGGGGAGTTTATGTATTTGCATCTCAATTTTCAGATCCACGATCGGATGCAGAATTCTGGAACAAATGGCACGAGTTTGAGGTGCAACACGGGAATGAAGATACGTTCAGAGAAATGCTTCGAATCAAACGGAGTGTTTCTGCAAGCTATAGCCAGACACACTTTATTCTACCTGAGTATATGATGCAAAAGGACCAGAGGTTGAGCATTGATGAGGCAAAAGACAAATTGAAACAGGCTGGAGTCCCCGAAGATGAAATGGCTGCTCTTGAGAGGCAGTTGGCTCCTGCGATCAGCGATACACCCTCTAAAGACAGCAACAGAAAAGTGGGCTTTGTGAGTGCTGGAATAGAATCACAGACTGATGGAGGGATAAAAGTTGCTGCAAATCACGAAGACATTGAACTACCAGAAGAAAGTGATTCTGAAGATGAGGCGAATGTTGAAATTGCACAAAAGGAGGTCCCAGCTGCTGTTTTTGGAGATTTGGCTAACAAGAGAAAGGATTCTGAAGATAATGAGGGTGGAGACAAGGATGGTGAGAGCCGCCTTGGTGCTCTTGAGAGAATAAAGAGGCTGAAGAAAGTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000349 GO:0000375 GO:0000377 GO:0000393 GO:0000398 GO:0000974 GO:0001701 GO:0001824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005681 GO:0005684 GO:0006139 GO:0006259 GO:0006281 GO:0006283 GO:0006289 GO:0006351 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007275 GO:0008150 GO:0008152 GO:0008380 GO:0009058 GO:0009059 GO:0009790 GO:0009792 GO:0009987 GO:0010467 GO:0016043 GO:0016070 GO:0016071 GO:0018130 GO:0019438 GO:0022607 GO:0022613 GO:0022618 GO:0032501 GO:0032502 GO:0032774 GO:0032991 GO:0033554 GO:0034622 GO:0034641 GO:0034645 GO:0034654 GO:0043009 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0048856 GO:0050896 GO:0051716 GO:0065003 GO:0071007 GO:0071010 GO:0071012 GO:0071013 GO:0071014 GO:0071704 GO:0071826 GO:0071840 GO:0090304 GO:0097659 GO:1901360 GO:1901362 GO:1901576 GO:1902494 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko03040 map03040

Pfam Domains

Protein Families

Suf

Protein Analysis

923

Amino Acids

107.42

Weight (kDa)

5.53

Isoelectric Point (pI)

49.85

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HAT_Syf1_CNRKL1_N	PF23233	12 - 170	1.3e-58	Pre-mRNA-splicing factor Syf1/CNRKL1 N-terminal HAT repeat
HAT_PRP39_N	PF23240	15 - 144	7.3e-09	PRP39 N-terminal HAT repeat
HAT_Syf1_M	PF23220	173 - 321	1.5e-58	Pre-mRNA-splicing factor SYF1 middle HAT repeat
HAT_Syf1_M	PF23220	360 - 423	9.1e-24	Pre-mRNA-splicing factor SYF1 middle HAT repeat
HAT_Syf1_CNRKL1_C	PF23231	425 - 819	2.8e-193	Pre-mRNA-splicing factor Syf1/CRNKL1 C-terminal HAT repeat
HAT_PRP39_C	PF23241	523 - 589	7.4e-06	PRP39 C-terminal HAT repeat
Suf	PF05843	528 - 671	6.7e-06	Suppressor of forked protein (Suf)
HAT_PRP39_N	PF23240	564 - 662	3.6e-07	PRP39 N-terminal HAT repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001333)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G28740
fragaria_vesca	FvH4_3g03910 FvH4_3g04150 FvH4_3g04150 FvH4_3g04150 FvH4_3g04150
malus_domestica	MD05G1324400.v1.1 MD10G1302200.v1.1
prunus_persica	Prupe.5G013200_v2.0.a1
pyrus_communis	pycom05g29860 pycom10g25470
rosa_chinensis	RchiOBHm_Chr5g0006181 RchiOBHm_Chr5g0006191 RchiOBHm_Chr5g0006201 RchiOBHm_Chr5g0006601 RchiOBHm_Chr7g0193971
rosa_laevigata	RLG00000004227 RLG00000031375 RLG00000031376 RLG00000031403
rosa_multiflora	Rmu_co7988368.1_g000001 Rmu_co8257165.1_g000001 Rmu_co8304153.1_g000001 Rmu_co8327759.1_g000001 Rmu_sc0001972.1_g000002 Rmu_sc0002389.1_g000006 Rmu_sc0004331.1_g000002 Rmu_sc0005583.1_g000001 Rmu_sc0009371.1_g000007 Rmu_sc0011154.1_g000005 Rmu_sc0024345.1_g000002 Rmu_sc0032392.1_g000001 Rmu_sc0032393.1_g000001
rosa_roxburghii	Rroxscaffold_1G00069420 Rroxscaffold_1G00069440 Rroxscaffold_1G00069800 Rroxscaffold_2G00122720 Rroxscaffold_3G00261670
rosa_rugosa	Rorug04G0422900
rosa_samantha	Rh5AG050200 Rh5BG046700 Rh5BG046800 Rh5BG049400 Rh5CG055400 Rh5CG055600 Rh5CG057800 Rh5DG046500 Rh5DG048800 Rh7AG139900 Rh7AG140000 Rh7DG141300
rosa_wichuraiana	Rw5G004790 Rw5G005020 Rw7G011940

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	761
Acc16I	TGCGCA	1 cut(s)	879
AccB1I	GGYRCC	1 cut(s)	1194
AccB7I	CCANNNNNTGG	1 cut(s)	1126
AccBSI	CCGCTC	1 cut(s)	574
AccI	GTMKAC	3 cut(s)	483, 1026, 1628
AccII	CGCG	4 cut(s)	119, 153, 204, 307
AccIII	TCCGGA	1 cut(s)	1014
AclWI	GGATC	6 cut(s)	105, 1265, 1278, 2050, 2063, 2207
AcsI	RAATTY	3 cut(s)	1456, 1811, 2231
AcuI	CTGAAG	2 cut(s)	2625, 2709
AcyI	GRCGYC	1 cut(s)	758
AfaI	GTAC	6 cut(s)	191, 251, 494, 1618, 1756, 1772
AfiI	CCNNNNNNNGG	5 cut(s)	276, 421, 477, 1126, 2067
AhdI	GACNNNNNGTC	1 cut(s)	2127
AjnI	CCWGG	5 cut(s)	356, 1251, 1380, 1470, 1611
AjuI	GAANNNNNNNTTGG	2 cut(s)	24, 56
Alw21I	GWGCWC	5 cut(s)	219, 394, 2148, 2179, 2737
Alw26I	GTCTC	3 cut(s)	1159, 2105, 2700
AlwI	GGATC	6 cut(s)	105, 1265, 1278, 2050, 2063, 2207
AlwNI	CAGNNNCTG	2 cut(s)	334, 2651
Ama87I	CYCGRG	4 cut(s)	212, 1632, 2147, 2178
Aor13HI	TCCGGA	1 cut(s)	1014
AoxI	GGCC	3 cut(s)	162, 579, 1374
ApeKI	GCWGC	7 cut(s)	403, 406, 1529, 2039, 2441, 2561, 2648
ApoI	RAATTY	3 cut(s)	1456, 1811, 2231
Asp700I	GAANNNNTTC	3 cut(s)	57, 2295, 2683
AspLEI	GCGC	8 cut(s)	96, 121, 135, 153, 155, 210, 385, 880
AspS9I	GGNCC	6 cut(s)	163, 1017, 1117, 1584, 2371, 2641
AsuC2I	CCSGG	2 cut(s)	171, 380
AsuHPI	GGTGA	4 cut(s)	346, 850, 1396, 2729
AsuII	TTCGAA	1 cut(s)	2299
AvaI	CYCGRG	4 cut(s)	212, 1632, 2147, 2178
AvaII	GGWCC	5 cut(s)	1017, 1117, 1584, 2371, 2641
BaeI	ACNNNNGTAYC	2 cut(s)	1892, 1925
BamHI	GGATCC	2 cut(s)	1270, 2055
BanI	GGYRCC	1 cut(s)	1194
BanII	GRGCYC	3 cut(s)	219, 1471, 2148
BauI	CACGAG	1 cut(s)	2249
BbsI	GAAGAC	5 cut(s)	629, 1141, 1268, 2126, 2580
Bbv12I	GWGCWC	5 cut(s)	219, 394, 2148, 2179, 2737
BbvI	GCAGC	7 cut(s)	415, 418, 1516, 2051, 2428, 2548, 2635
BccI	CCATC	6 cut(s)	149, 904, 1128, 1529, 2537, 2708
BceAI	ACGGC	4 cut(s)	140, 693, 1361, 1486
BcgI	CGANNNNNNTGC	2 cut(s)	857, 891
BciT130I	CCWGG	5 cut(s)	358, 1253, 1382, 1472, 1613
BciVI	GTATCC	1 cut(s)	450
BclI	TGATCA	1 cut(s)	598
BcnI	CCSGG	2 cut(s)	171, 380
BcoDI	GTCTC	3 cut(s)	1159, 2105, 2700
BfaI	CTAG	5 cut(s)	804, 1110, 1413, 1622, 1946
BfmI	CTRYAG	3 cut(s)	73, 1491, 2326
BfoI	RGCGCY	4 cut(s)	97, 136, 211, 386
BfuI	GTATCC	1 cut(s)	450
BglI	GCCNNNNNGGC	1 cut(s)	161
BlpI	GCTNAGC	3 cut(s)	1041, 1320, 2142
Bme1390I	CCNGG	7 cut(s)	171, 358, 380, 1253, 1382, 1472, 1613
Bme18I	GGWCC	5 cut(s)	1017, 1117, 1584, 2371, 2641
BmeRI	GACNNNNNGTC	1 cut(s)	2127
BmeT110I	CYCGRG	4 cut(s)	212, 1632, 2147, 2178
BmgT120I	GGNCC	6 cut(s)	163, 1017, 1117, 1584, 2371, 2641
BmiI	GGNNCC	7 cut(s)	1196, 1272, 1562, 1884, 2057, 2463, 2643
BmrFI	CCNGG	7 cut(s)	171, 358, 380, 1253, 1382, 1472, 1613
BpiI	GAAGAC	5 cut(s)	629, 1141, 1268, 2126, 2580
BpmI	CTGGAG	2 cut(s)	1949, 2439
Bpu1102I	GCTNAGC	3 cut(s)	1041, 1320, 2142
Bpu14I	TTCGAA	1 cut(s)	2299
BpuEI	CTTGAG	5 cut(s)	65, 1067, 1594, 2468, 2759
BpuMI	CCSGG	2 cut(s)	171, 380
BsaBI	GATNNNNATC	2 cut(s)	461, 1539
BsaHI	GRCGYC	1 cut(s)	758
BsaI	GGTCTC	1 cut(s)	2105
BsaJI	CCNNGG	9 cut(s)	169, 305, 582, 675, 1063, 1251, 1470, 1612, 2728
BsaWI	WCCGGW	5 cut(s)	397, 414, 486, 1014, 1197
BsaXI	ACNNNNNCTCC	2 cut(s)	2175, 2205
Bsc4I	CCNNNNNNNGG	5 cut(s)	276, 421, 477, 1126, 2067
Bse1I	ACTGG	2 cut(s)	1149, 1547
Bse3DI	GCAATG	2 cut(s)	1196, 1697
Bse8I	GATNNNNATC	2 cut(s)	461, 1539
BseAI	TCCGGA	1 cut(s)	1014
BseBI	CCWGG	5 cut(s)	358, 1253, 1382, 1472, 1613
BseDI	CCNNGG	9 cut(s)	169, 305, 582, 675, 1063, 1251, 1470, 1612, 2728
BseGI	GGATG	7 cut(s)	706, 742, 814, 831, 1873, 2230, 2719
BseJI	GATNNNNATC	2 cut(s)	461, 1539
BseLI	CCNNNNNNNGG	5 cut(s)	276, 421, 477, 1126, 2067
BseMI	GCAATG	2 cut(s)	1196, 1697
BseMII	CTCAG	6 cut(s)	26, 540, 1032, 2034, 2133, 2343
BseNI	ACTGG	2 cut(s)	1149, 1547
BsePI	GCGCGC	1 cut(s)	151
BseXI	GCAGC	7 cut(s)	415, 418, 1516, 2051, 2428, 2548, 2635
BseYI	CCCAGC	1 cut(s)	2644
BsgI	GTGCAG	2 cut(s)	422, 2058
Bsh1236I	CGCG	4 cut(s)	119, 153, 204, 307
Bsh1285I	CGRYCG	2 cut(s)	415, 2222
BshFI	GGCC	3 cut(s)	164, 581, 1376
BshNI	GGYRCC	1 cut(s)	1194
BsiEI	CGRYCG	2 cut(s)	415, 2222
BsiHKAI	GWGCWC	5 cut(s)	219, 394, 2148, 2179, 2737
BsiHKCI	CYCGRG	4 cut(s)	212, 1632, 2147, 2178
BsiSI	CCGG	8 cut(s)	171, 367, 379, 398, 415, 487, 1015, 1198
BslFI	GGGAC	2 cut(s)	2408, 2627
BslI	CCNNNNNNNGG	5 cut(s)	276, 421, 477, 1126, 2067
BsmAI	GTCTC	3 cut(s)	1159, 2105, 2700
BsmFI	GGGAC	2 cut(s)	2408, 2627
BsmI	GAATGC	2 cut(s)	676, 1733
BsnI	GGCC	3 cut(s)	164, 581, 1376
Bso31I	GGTCTC	1 cut(s)	2105
BsoBI	CYCGRG	4 cut(s)	212, 1632, 2147, 2178
Bsp119I	TTCGAA	1 cut(s)	2299
Bsp1286I	GDGCHC	6 cut(s)	219, 394, 1471, 2148, 2179, 2737
Bsp13I	TCCGGA	1 cut(s)	1014
Bsp143I	GATC	9 cut(s)	110, 234, 598, 1270, 2055, 2170, 2212, 2219, 2469
Bsp1720I	GCTNAGC	3 cut(s)	1041, 1320, 2142
Bsp19I	CCATGG	1 cut(s)	1063
BspANI	GGCC	3 cut(s)	164, 581, 1376
BspCNI	CTCAG	6 cut(s)	25, 541, 1033, 2035, 2134, 2344
BspEI	TCCGGA	1 cut(s)	1014
BspFNI	CGCG	4 cut(s)	119, 153, 204, 307
BspLI	GGNNCC	7 cut(s)	1196, 1272, 1562, 1884, 2057, 2463, 2643
BspPI	GGATC	6 cut(s)	105, 1265, 1278, 2050, 2063, 2207
BspQI	GCTCTTC	3 cut(s)	48, 399, 2147
BspT104I	TTCGAA	1 cut(s)	2299
BspT107I	GGYRCC	1 cut(s)	1194
BspTNI	GGTCTC	1 cut(s)	2105
BsrBI	CCGCTC	1 cut(s)	574
BsrDI	GCAATG	2 cut(s)	1196, 1697
BsrI	ACTGG	2 cut(s)	1149, 1547
BssECI	CCNNGG	9 cut(s)	169, 305, 582, 675, 1063, 1251, 1470, 1612, 2728
BssHII	GCGCGC	1 cut(s)	151
BssMI	GATC	9 cut(s)	110, 234, 598, 1270, 2055, 2170, 2212, 2219, 2469
BssNI	GRCGYC	1 cut(s)	758
BssSI	CACGAG	1 cut(s)	2249
BssT1I	CCWWGG	2 cut(s)	1063, 2728
Bst2BI	CACGAG	1 cut(s)	2249
Bst2UI	CCWGG	5 cut(s)	358, 1253, 1382, 1472, 1613
Bst4CI	ACNGT	4 cut(s)	835, 844, 1267, 1399
Bst6I	CTCTTC	6 cut(s)	48, 399, 882, 990, 1516, 2147
BstACI	GRCGYC	1 cut(s)	758
BstAPI	GCANNNNNTGC	1 cut(s)	301
BstBI	TTCGAA	1 cut(s)	2299
BstC8I	GCNNGC	8 cut(s)	153, 206, 579, 900, 1281, 1956, 2085, 2323
BstDEI	CTNAG	9 cut(s)	12, 549, 666, 1041, 1320, 1983, 2043, 2142, 2352
BstDSI	CCRYGG	3 cut(s)	305, 675, 1063
BstEII	GGTNACC	1 cut(s)	352
BstENI	CCTNNNNNAGG	1 cut(s)	274
BstF5I	GGATG	7 cut(s)	706, 742, 814, 831, 1873, 2230, 2719
BstFNI	CGCG	4 cut(s)	119, 153, 204, 307
BstH2I	RGCGCY	4 cut(s)	97, 136, 211, 386
BstHHI	GCGC	8 cut(s)	96, 121, 135, 153, 155, 210, 385, 880
BstKTI	GATC	9 cut(s)	113, 237, 601, 1273, 2058, 2173, 2215, 2222, 2472
BstMAI	GTCTC	3 cut(s)	1159, 2105, 2700
BstMBI	GATC	9 cut(s)	110, 234, 598, 1270, 2055, 2170, 2212, 2219, 2469
BstMCI	CGRYCG	2 cut(s)	415, 2222
BstNI	CCWGG	5 cut(s)	358, 1253, 1382, 1472, 1613
BstNSI	RCATGY	2 cut(s)	1781, 2017
BstPI	GGTNACC	1 cut(s)	352
BstSCI	CCNGG	7 cut(s)	169, 356, 378, 1251, 1380, 1470, 1611
BstSFI	CTRYAG	3 cut(s)	73, 1491, 2326
BstUI	CGCG	4 cut(s)	119, 153, 204, 307
BstV1I	GCAGC	7 cut(s)	415, 418, 1516, 2051, 2428, 2548, 2635
BstV2I	GAAGAC	5 cut(s)	629, 1141, 1268, 2126, 2580
BstX2I	RGATCY	3 cut(s)	1270, 2055, 2212
BstYI	RGATCY	3 cut(s)	1270, 2055, 2212
BsuI	GTATCC	1 cut(s)	450
BsuRI	GGCC	3 cut(s)	164, 581, 1376
BtgI	CCRYGG	3 cut(s)	305, 675, 1063
BtsCI	GGATG	7 cut(s)	706, 742, 814, 831, 1873, 2230, 2719
BtsI	GCAGTG	1 cut(s)	1290
BtsIMutI	CAGTG	2 cut(s)	1272, 1290
Cac8I	GCNNGC	8 cut(s)	153, 206, 579, 900, 1281, 1956, 2085, 2323
CaiI	CAGNNNCTG	2 cut(s)	334, 2651
CfoI	GCGC	8 cut(s)	96, 121, 135, 153, 155, 210, 385, 880
Cfr13I	GGNCC	6 cut(s)	163, 1017, 1117, 1584, 2371, 2641
Cfr42I	CCGCGG	1 cut(s)	308
CseI	GACGC	1 cut(s)	125
Csp6I	GTAC	6 cut(s)	190, 250, 493, 1617, 1755, 1771
CviAII	CATG	7 cut(s)	515, 1064, 1079, 1337, 1778, 2000, 2014
CviQI	GTAC	6 cut(s)	190, 250, 493, 1617, 1755, 1771
DdeI	CTNAG	9 cut(s)	12, 549, 666, 1041, 1320, 1983, 2043, 2142, 2352
DpnI	GATC	9 cut(s)	112, 236, 600, 1272, 2057, 2172, 2214, 2221, 2471
DpnII	GATC	9 cut(s)	110, 234, 598, 1270, 2055, 2170, 2212, 2219, 2469
DraI	TTTAAA	1 cut(s)	1462
DriI	GACNNNNNGTC	1 cut(s)	2127
Eam1104I	CTCTTC	6 cut(s)	48, 399, 882, 990, 1516, 2147
Eam1105I	GACNNNNNGTC	1 cut(s)	2127
EarI	CTCTTC	6 cut(s)	48, 399, 882, 990, 1516, 2147
EciI	GGCGGA	2 cut(s)	584, 782
Ecl136II	GAGCTC	2 cut(s)	217, 2146
Eco130I	CCWWGG	2 cut(s)	1063, 2728
Eco24I	GRGCYC	3 cut(s)	219, 1471, 2148
Eco31I	GGTCTC	1 cut(s)	2105
Eco47I	GGWCC	5 cut(s)	1017, 1117, 1584, 2371, 2641
Eco53kI	GAGCTC	2 cut(s)	217, 2146
Eco57I	CTGAAG	2 cut(s)	2625, 2709
Eco88I	CYCGRG	4 cut(s)	212, 1632, 2147, 2178
Eco91I	GGTNACC	1 cut(s)	352
EcoICRI	GAGCTC	2 cut(s)	217, 2146
EcoNI	CCTNNNNNAGG	1 cut(s)	274
EcoO109I	RGGNCCY	2 cut(s)	163, 2641
EcoO65I	GGTNACC	1 cut(s)	352
EcoRI	GAATTC	1 cut(s)	2231
EcoRII	CCWGG	5 cut(s)	356, 1251, 1380, 1470, 1611
EcoT14I	CCWWGG	2 cut(s)	1063, 2728
EcoT22I	ATGCAT	2 cut(s)	1699, 1735
EcoT38I	GRGCYC	3 cut(s)	219, 1471, 2148
ErhI	CCWWGG	2 cut(s)	1063, 2728
FaeI	CATG	7 cut(s)	518, 1067, 1082, 1340, 1781, 2003, 2017
FalI	AAGNNNNNCTT	4 cut(s)	24, 56, 42, 74
FaqI	GGGAC	2 cut(s)	2408, 2627
FatI	CATG	7 cut(s)	514, 1063, 1078, 1336, 1777, 1999, 2013
FauI	CCCGC	2 cut(s)	1782, 1947
FauNDI	CATATG	1 cut(s)	1790
FbaI	TGATCA	1 cut(s)	598
FblI	GTMKAC	3 cut(s)	483, 1026, 1628
FokI	GGATG	7 cut(s)	713, 749, 821, 838, 1880, 2237, 2726
FriOI	GRGCYC	3 cut(s)	219, 1471, 2148
FspBI	CTAG	5 cut(s)	804, 1110, 1413, 1622, 1946
FspI	TGCGCA	1 cut(s)	879
GlaI	GCGC	8 cut(s)	95, 120, 134, 152, 154, 209, 384, 879
GsaI	CCCAGC	1 cut(s)	2648
GsuI	CTGGAG	2 cut(s)	1949, 2439
HaeII	RGCGCY	4 cut(s)	97, 136, 211, 386
HaeIII	GGCC	3 cut(s)	164, 581, 1376
HapII	CCGG	8 cut(s)	171, 367, 379, 398, 415, 487, 1015, 1198
HgaI	GACGC	1 cut(s)	125
HhaI	GCGC	8 cut(s)	96, 121, 135, 153, 155, 210, 385, 880
Hin1I	GRCGYC	1 cut(s)	758
Hin1II	CATG	7 cut(s)	518, 1067, 1082, 1340, 1781, 2003, 2017
Hin6I	GCGC	8 cut(s)	94, 119, 133, 151, 153, 208, 383, 878
HinP1I	GCGC	8 cut(s)	94, 119, 133, 151, 153, 208, 383, 878
HpaII	CCGG	8 cut(s)	171, 367, 379, 398, 415, 487, 1015, 1198
HphI	GGTGA	4 cut(s)	346, 850, 1396, 2729
Hpy166II	GTNNAC	8 cut(s)	484, 732, 756, 1027, 1387, 1597, 1619, 1629
Hpy8I	GTNNAC	8 cut(s)	484, 732, 756, 1027, 1387, 1597, 1619, 1629
Hpy99I	CGWCG	2 cut(s)	119, 763
HpyAV	CCTTC	8 cut(s)	50, 133, 280, 445, 741, 793, 953, 1957
HpyCH4III	ACNGT	4 cut(s)	835, 844, 1267, 1399
HpyCH4IV	ACGT	4 cut(s)	758, 1301, 1846, 2282
HpyF3I	CTNAG	9 cut(s)	12, 549, 666, 1041, 1320, 1983, 2043, 2142, 2352
HpySE526I	ACGT	4 cut(s)	758, 1301, 1846, 2282
Hsp92I	GRCGYC	1 cut(s)	758
Hsp92II	CATG	7 cut(s)	518, 1067, 1082, 1340, 1781, 2003, 2017
HspAI	GCGC	8 cut(s)	94, 119, 133, 151, 153, 208, 383, 878
Kpn2I	TCCGGA	1 cut(s)	1014
Ksp22I	TGATCA	1 cut(s)	598
KspI	CCGCGG	1 cut(s)	308
Kzo9I	GATC	9 cut(s)	110, 234, 598, 1270, 2055, 2170, 2212, 2219, 2469
LguI	GCTCTTC	3 cut(s)	48, 399, 2147
LmnI	GCTCC	6 cut(s)	340, 424, 571, 1566, 1888, 2467
Lsp1109I	GCAGC	7 cut(s)	415, 418, 1516, 2051, 2428, 2548, 2635
MaeI	CTAG	5 cut(s)	804, 1110, 1413, 1622, 1946
MaeII	ACGT	4 cut(s)	758, 1301, 1846, 2282
MaeIII	GTNAC	3 cut(s)	286, 352, 838
MalI	GATC	9 cut(s)	112, 236, 600, 1272, 2057, 2172, 2214, 2221, 2471
MbiI	CCGCTC	1 cut(s)	574
MboI	GATC	9 cut(s)	110, 234, 598, 1270, 2055, 2170, 2212, 2219, 2469
MflI	RGATCY	3 cut(s)	1270, 2055, 2212
MhlI	GDGCHC	6 cut(s)	219, 394, 1471, 2148, 2179, 2737
MlyI	GAGTC	5 cut(s)	355, 505, 554, 1634, 2430
MmeI	TCCRAC	2 cut(s)	138, 624
Mph1103I	ATGCAT	2 cut(s)	1699, 1735
MroI	TCCGGA	1 cut(s)	1014
MroXI	GAANNNNTTC	3 cut(s)	57, 2295, 2683
MseI	TTAA	7 cut(s)	591, 615, 723, 780, 906, 1461, 1875
MslI	CAYNNNNRTG	1 cut(s)	2271
MspA1I	CMGCKG	3 cut(s)	307, 2042, 2648
MspI	CCGG	8 cut(s)	171, 367, 379, 398, 415, 487, 1015, 1198
MspR9I	CCNGG	7 cut(s)	171, 358, 380, 1253, 1382, 1472, 1613
Mva1269I	GAATGC	2 cut(s)	676, 1733
MvaI	CCWGG	5 cut(s)	358, 1253, 1382, 1472, 1613
MvnI	CGCG	4 cut(s)	119, 153, 204, 307
NciI	CCSGG	2 cut(s)	171, 380
NcoI	CCATGG	1 cut(s)	1063
NdeI	CATATG	1 cut(s)	1790
NdeII	GATC	9 cut(s)	110, 234, 598, 1270, 2055, 2170, 2212, 2219, 2469
NlaIII	CATG	7 cut(s)	518, 1067, 1082, 1340, 1781, 2003, 2017
NlaIV	GGNNCC	7 cut(s)	1196, 1272, 1562, 1884, 2057, 2463, 2643
NmuCI	GTSAC	3 cut(s)	286, 352, 838
NsbI	TGCGCA	1 cut(s)	879
NsiI	ATGCAT	2 cut(s)	1699, 1735
NspI	RCATGY	2 cut(s)	1781, 2017
NspV	TTCGAA	1 cut(s)	2299
PaeR7I	CTCGAG	2 cut(s)	212, 2147
PauI	GCGCGC	1 cut(s)	151
PciSI	GCTCTTC	3 cut(s)	48, 399, 2147
PcsI	WCGNNNNNNNCGW	2 cut(s)	111, 153
PctI	GAATGC	2 cut(s)	676, 1733
PdmI	GAANNNNTTC	3 cut(s)	57, 2295, 2683
PfeI	GAWTC	8 cut(s)	316, 1222, 1540, 2093, 2301, 2531, 2600, 2684
PflMI	CCANNNNNTGG	1 cut(s)	1126
Ple19I	CGATCG	1 cut(s)	2222
PleI	GAGTC	5 cut(s)	355, 505, 553, 1633, 2429
PpsI	GAGTC	5 cut(s)	355, 505, 553, 1633, 2429
PpuMI	RGGWCCY	1 cut(s)	2641
Psp124BI	GAGCTC	2 cut(s)	219, 2148
Psp5II	RGGWCCY	1 cut(s)	2641
Psp6I	CCWGG	5 cut(s)	356, 1251, 1380, 1470, 1611
PspEI	GGTNACC	1 cut(s)	352
PspFI	CCCAGC	1 cut(s)	2644
PspGI	CCWGG	5 cut(s)	356, 1251, 1380, 1470, 1611
PspN4I	GGNNCC	7 cut(s)	1196, 1272, 1562, 1884, 2057, 2463, 2643
PspPI	GGNCC	6 cut(s)	163, 1017, 1117, 1584, 2371, 2641
PspPPI	RGGWCCY	1 cut(s)	2641
PspXI	VCTCGAGB	1 cut(s)	212
PsrI	GAACNNNNNNTAC	2 cut(s)	422, 454
PstNI	CAGNNNCTG	2 cut(s)	334, 2651
PsuI	RGATCY	3 cut(s)	1270, 2055, 2212
PteI	GCGCGC	1 cut(s)	151
PvuI	CGATCG	1 cut(s)	2222
PvuII	CAGCTG	2 cut(s)	2042, 2648
RsaI	GTAC	6 cut(s)	191, 251, 494, 1618, 1756, 1772
RsaNI	GTAC	6 cut(s)	190, 250, 493, 1617, 1755, 1771
RseI	CAYNNNNRTG	1 cut(s)	2271
SacI	GAGCTC	2 cut(s)	219, 2148
SacII	CCGCGG	1 cut(s)	308
SapI	GCTCTTC	3 cut(s)	48, 399, 2147
SaqAI	TTAA	7 cut(s)	591, 615, 723, 780, 906, 1461, 1875
Sau3AI	GATC	9 cut(s)	110, 234, 598, 1270, 2055, 2170, 2212, 2219, 2469
Sau96I	GGNCC	6 cut(s)	163, 1017, 1117, 1584, 2371, 2641
SchI	GAGTC	5 cut(s)	355, 505, 554, 1634, 2430
ScrFI	CCNGG	7 cut(s)	171, 358, 380, 1253, 1382, 1472, 1613
SduI	GDGCHC	6 cut(s)	219, 394, 1471, 2148, 2179, 2737
SfcI	CTRYAG	3 cut(s)	73, 1491, 2326
Sfr274I	CTCGAG	2 cut(s)	212, 2147
Sfr303I	CCGCGG	1 cut(s)	308
SfuI	TTCGAA	1 cut(s)	2299
SgrBI	CCGCGG	1 cut(s)	308
SinI	GGWCC	5 cut(s)	1017, 1117, 1584, 2371, 2641
SlaI	CTCGAG	2 cut(s)	212, 2147
SmiMI	CAYNNNNRTG	1 cut(s)	2271
SmlI	CTYRAG	7 cut(s)	80, 212, 1046, 1573, 2147, 2447, 2738
SmoI	CTYRAG	7 cut(s)	80, 212, 1046, 1573, 2147, 2447, 2738
SspI	AATATT	1 cut(s)	1764
SspMI	CTAG	5 cut(s)	804, 1110, 1413, 1622, 1946
SstI	GAGCTC	2 cut(s)	219, 2148
StyD4I	CCNGG	7 cut(s)	169, 356, 378, 1251, 1380, 1470, 1611
StyI	CCWWGG	2 cut(s)	1063, 2728
TaaI	ACNGT	4 cut(s)	835, 844, 1267, 1399
TaiI	ACGT	4 cut(s)	761, 1304, 1849, 2285
TaqI	TCGA	6 cut(s)	213, 273, 867, 1842, 2148, 2299
TauI	GCSGC	7 cut(s)	138, 307, 569, 577, 1450, 1883, 2727
TfiI	GAWTC	8 cut(s)	316, 1222, 1540, 2093, 2301, 2531, 2600, 2684
Tru1I	TTAA	7 cut(s)	591, 615, 723, 780, 906, 1461, 1875
Tru9I	TTAA	7 cut(s)	591, 615, 723, 780, 906, 1461, 1875
TscAI	CASTG	2 cut(s)	1272, 1290
TseFI	GTSAC	3 cut(s)	286, 352, 838
TseI	GCWGC	7 cut(s)	403, 406, 1529, 2039, 2441, 2561, 2648
Tsp45I	GTSAC	3 cut(s)	286, 352, 838
TspDTI	ATGAA	8 cut(s)	1344, 1761, 1976, 1989, 2016, 2034, 2289, 2448
TspGWI	ACGGA	3 cut(s)	353, 749, 2323
TspRI	CASTG	2 cut(s)	1272, 1290
Van91I	CCANNNNNTGG	1 cut(s)	1126
VpaK11BI	GGWCC	5 cut(s)	1017, 1117, 1584, 2371, 2641
XagI	CCTNNNNNAGG	1 cut(s)	274
XapI	RAATTY	3 cut(s)	1456, 1811, 2231
XceI	RCATGY	2 cut(s)	1781, 2017
XhoI	CTCGAG	2 cut(s)	212, 2147
XmiI	GTMKAC	3 cut(s)	483, 1026, 1628
XmnI	GAANNNNTTC	3 cut(s)	57, 2295, 2683
XspI	CTAG	5 cut(s)	804, 1110, 1413, 1622, 1946
ZraI	GACGTC	1 cut(s)	759
Zsp2I	ATGCAT	2 cut(s)	1699, 1735

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.