Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5B

Orthogroup ID

OG0001333

Physical Location & Seq

Genomic Coordinates Forward (+)

3920172 .. 3924616

Gene Structure 4445 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5BG049400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2760 bp

ATGGCTATCTCTCAAGAACTCTACCCCTCCCAAGACGACCTCCTCTACGAAGAAGAGCTTCTCCGAAACCCTTACAGCCTCAAGCTCTGGTGGCGCTACCTAATCGCCCGATCCGACGCCCCCTTCAAGAAGCGCCGCACCATCTACGAGCGCGCCGTCAAGGCCCTCCCCGGAAGCTACAAGCTCTGGTACGCCTACCTCCGCGAGCGCCTCGAGCTCGTCCGCAACTTTCCGATCAACCACTCCGAGTACGAAACCCTAAACAACACCTTCGAGAGGGCGTTGGTCACAATGCACAAGATGCCGCGGATTTGGATTCTGTATTTACAGAGCCTGACGGAGCAGAGATTGGTCACCAGGACTCGCCGGAGTTTTGACCGGGCGCTGTGTGCTCTGCCGGTGCAGCAGCACGACCGGATTTGGGAGCTGTATTTGGTGTTCGTGAGCCAGAAGGGGATCCCTATCCAGACCTCGCTTCGGGTCTACCGGAGGTACTTGCTTTATGACCCGACTCATGTTGAGGATTTTATTGCGTTTTTGATAGAGTCTGAGCTGTGGCAAGAGGCGGCGGAGCGGCTGGCCTCGGTGCTTAATGATGATCAGTTTAGGTCAATTAAGGGGAAGACGAAGCATAGGCTGTGGTTGGAGTTGTGTGATTTGCTTACTAAGAATGCCACGGCGGTTTCGGGGTTGAATGTGGATGCTATCATTAGAGGTGGGATTAAGAAGTTTACAGATGAGGTGGGGAGGCTGTGGACGTCGTTGGCGGATTATTATATTAAGAGGAGTTTGTTTGAGAAGGCTCGGGATGTGTTTGAGGAGGGTATGCAGACTGTGGTGACTGTGAGGGATTTCAGTGTGATTTTCGATGCGTATGCGCAGTTTGAAGAGAGTATGCTTGCGATTAAGATGGAGGCTTTGGGAAGTGATGAGGAGGAGGAGGAGGAGGAAGGAGAGAATGGGAGAATGGAAGATGAGAGAAGTGAAGAGGAGGAGGAAGATGTCCGGACCAATGTAGACTTGTCTGTGGCTGAGCTTGAGAGGAAAATACTCCATGGGTTTTGGCTACATGATGAGAATGATGTTGATTTGAGGTTGGCTAGATTGGACCATCTTATGGATAGAAGACCCGAACTGGCTAATAGTGTTCTTTTGAGACAAAATCCGCATAATGTAGAGCAATGGCACCGGAGAGTGAAGCTGTTTGAGGGGAATCCTACAAAGCAGATACTTACTTACACCCAGGCTGTGAAGACAGTGGATCCAATGCAAGCAGTGGGGAAGCCTCATACGTTGTGGGTTGCTTTTGCTAAGCTGTATGAAACACATGGAGATATTGCAAATGCAAGAGTAATTTTTGATAAGGCCGTTCAGGTGAACTACAAGACAGTGGATAATCTGGCTAGTCTATGGTGTGAGTGGGCAGAAATGGAATTGCGGCATAAGAATTTTAAACGAGCCCTGGAACTGATGAGTAGAGCTACAGCAGAGCCGTCTGTTGAGGTCAAACGAAGAGTTGCTGCTGATGGGAATCCACCAGTTCAGATGAGGCTCCATAAATCCTTGAGGCTTTGGACCTTTTATGTGGACTTGGAGGAGAGCCTGGGTACACTAGAGTCTACTCGGGCAGTGTATGAGAGGATATTGGATTTGAGAATAGCCACACCTCAAATAATAATCAACTATGCATTGCTTCTGGAAGAACATAAATACTTTGAAGATGCATTCAAAGTCTATGAAAAGGGTACTCAAATATTCAAGTACCCGCATGTCAAAGACATATGGATTACATATCTCTCTAAATTTGTAAAAAGATATGGGAAGAATAAGCTCGAACGTGCAAGACTGCTCTTTGAGGATGCTGTTAAAGCGGCTCCTGCTGATGCGAAGAAACCTTTGTATCTTCAATTTGCAAAGCTGGAGGAGGATTATGGTCTAGCAAAGCGGGCTATGAAGGTCTATGATGAAGCAACTAAGGCTGTTCCAAACCATGAAAAGTTGAGCATGTATGAAATCTATATTGCCCGTGCAGCTGAGATATTTGGGATCCCCAAAACAAGGGAGATATATGAGCAAGCAATAGAATCTGGTCTCCCTGACAAAGATGTGAAGACAATGTGTCTAAAGTATGCTGAGCTCGAGAAGAGCTTGGGCGAAATTGATCGTGCTCGGGGAGTTTATGTATTTGCATCTCAATTTTCAGATCCACGATCGGATGCAGAATTCTGGAACAAATGGCACGAGTTTGAGGTGCAACATGGGAATGAAGATACGTTCAGAGAAATGCTTCGAATCAAACGGAGTGTTTCTGCAAGCTATAGCCAGACACACTTTATTCTACCTGAGTATATGATGCAAAAGGACCAGAGGTTGAGCATTGATGAGGCAAAAGACAAATTGAAACAGGCTGGAGTCCCCGAAGATGAAATGGCTGCCCTTGAGGGGCAGTTGGCTCCTGCGATCAGCGATACACCCTCTAAAGACAGCAACAGAAAAGTGGGCTTTGTGAGTGCTGGAATAGAATCACAGACTGATGGAGGGATAAAAGTTGCTGCAAATCACGAAGACATTGAACTACCAGAAGAAAGTGATTCTGAAGATGAGGCGAATGTTGAAATTGCACAAAAGGAGGTCCCAGCTGCTGTTTTTGGAGATTTGGCTAACAAGAGAAAGGATTCTGAAGATAATGAGGGCGGAGACAAGGATGGTGAGAGCCGCCTTGGTGCTCTTGAGAGAATAAAGAGGCTGAAGAAAGTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000349 GO:0000375 GO:0000377 GO:0000393 GO:0000398 GO:0000974 GO:0001701 GO:0001824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005681 GO:0005684 GO:0006139 GO:0006259 GO:0006281 GO:0006283 GO:0006289 GO:0006351 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007275 GO:0008150 GO:0008152 GO:0008380 GO:0009058 GO:0009059 GO:0009790 GO:0009792 GO:0009987 GO:0010467 GO:0016043 GO:0016070 GO:0016071 GO:0018130 GO:0019438 GO:0022607 GO:0022613 GO:0022618 GO:0032501 GO:0032502 GO:0032774 GO:0032991 GO:0033554 GO:0034622 GO:0034641 GO:0034645 GO:0034654 GO:0043009 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0048856 GO:0050896 GO:0051716 GO:0065003 GO:0071007 GO:0071010 GO:0071012 GO:0071013 GO:0071014 GO:0071704 GO:0071826 GO:0071840 GO:0090304 GO:0097659 GO:1901360 GO:1901362 GO:1901576 GO:1902494 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko03040 map03040

Pfam Domains

Protein Families

Suf

Protein Analysis

919

Amino Acids

106.9

Weight (kDa)

5.6

Isoelectric Point (pI)

48.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HAT_Syf1_CNRKL1_N	PF23233	12 - 170	1.3e-58	Pre-mRNA-splicing factor Syf1/CNRKL1 N-terminal HAT repeat
HAT_PRP39_N	PF23240	15 - 144	7.3e-09	PRP39 N-terminal HAT repeat
HAT_Syf1_M	PF23220	173 - 318	2e-58	Pre-mRNA-splicing factor SYF1 middle HAT repeat
HAT_Syf1_M	PF23220	357 - 420	9.3e-24	Pre-mRNA-splicing factor SYF1 middle HAT repeat
HAT_Syf1_CNRKL1_C	PF23231	422 - 816	9e-193	Pre-mRNA-splicing factor Syf1/CRNKL1 C-terminal HAT repeat
HAT_PRP39_C	PF23241	520 - 586	7.4e-06	PRP39 C-terminal HAT repeat
Suf	PF05843	525 - 668	6.6e-06	Suppressor of forked protein (Suf)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001333)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G28740
fragaria_vesca	FvH4_3g03910 FvH4_3g04150 FvH4_3g04150 FvH4_3g04150 FvH4_3g04150
malus_domestica	MD05G1324400.v1.1 MD10G1302200.v1.1
prunus_persica	Prupe.5G013200_v2.0.a1
pyrus_communis	pycom05g29860 pycom10g25470
rosa_chinensis	RchiOBHm_Chr5g0006181 RchiOBHm_Chr5g0006191 RchiOBHm_Chr5g0006201 RchiOBHm_Chr5g0006601 RchiOBHm_Chr7g0193971
rosa_laevigata	RLG00000004227 RLG00000031375 RLG00000031376 RLG00000031403
rosa_multiflora	Rmu_co7988368.1_g000001 Rmu_co8257165.1_g000001 Rmu_co8304153.1_g000001 Rmu_co8327759.1_g000001 Rmu_sc0001972.1_g000002 Rmu_sc0002389.1_g000006 Rmu_sc0004331.1_g000002 Rmu_sc0005583.1_g000001 Rmu_sc0009371.1_g000007 Rmu_sc0011154.1_g000005 Rmu_sc0024345.1_g000002 Rmu_sc0032392.1_g000001 Rmu_sc0032393.1_g000001
rosa_roxburghii	Rroxscaffold_1G00069420 Rroxscaffold_1G00069440 Rroxscaffold_1G00069800 Rroxscaffold_2G00122720 Rroxscaffold_3G00261670
rosa_rugosa	Rorug04G0422900
rosa_samantha	Rh5AG050200 Rh5BG046700 Rh5BG046800 Rh5BG049400 Rh5CG055400 Rh5CG055600 Rh5CG057800 Rh5DG046500 Rh5DG048800 Rh7AG139900 Rh7AG140000 Rh7DG141300
rosa_wichuraiana	Rw5G004790 Rw5G005020 Rw7G011940

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	761
Acc16I	TGCGCA	1 cut(s)	879
AccB1I	GGYRCC	1 cut(s)	1185
AccB7I	CCANNNNNTGG	1 cut(s)	1117
AccBSI	CCGCTC	1 cut(s)	574
AccI	GTMKAC	3 cut(s)	483, 1017, 1619
AccII	CGCG	3 cut(s)	153, 204, 307
AccIII	TCCGGA	1 cut(s)	1005
AclWI	GGATC	8 cut(s)	105, 451, 464, 1256, 1269, 2041, 2054, 2198
AcsI	RAATTY	3 cut(s)	1447, 1802, 2222
AcuI	CTGAAG	2 cut(s)	2616, 2700
AcyI	GRCGYC	2 cut(s)	117, 758
AfaI	GTAC	6 cut(s)	191, 251, 494, 1609, 1747, 1763
AfiI	CCNNNNNNNGG	6 cut(s)	276, 421, 477, 1117, 2058, 2442
AhdI	GACNNNNNGTC	1 cut(s)	2118
AjnI	CCWGG	4 cut(s)	356, 1242, 1461, 1602
Alw21I	GWGCWC	5 cut(s)	219, 394, 2139, 2170, 2728
Alw26I	GTCTC	3 cut(s)	1150, 2096, 2691
AlwI	GGATC	8 cut(s)	105, 451, 464, 1256, 1269, 2041, 2054, 2198
AlwNI	CAGNNNCTG	2 cut(s)	334, 2642
Ama87I	CYCGRG	5 cut(s)	212, 804, 1623, 2138, 2169
Aor13HI	TCCGGA	1 cut(s)	1005
AoxI	GGCC	3 cut(s)	162, 579, 1365
ApeKI	GCWGC	7 cut(s)	403, 406, 1520, 2030, 2432, 2552, 2639
ApoI	RAATTY	3 cut(s)	1447, 1802, 2222
Asp700I	GAANNNNTTC	3 cut(s)	57, 2286, 2674
AspLEI	GCGC	7 cut(s)	96, 135, 153, 155, 210, 385, 880
AspS9I	GGNCC	6 cut(s)	163, 1008, 1108, 1575, 2362, 2632
AsuC2I	CCSGG	2 cut(s)	171, 380
AsuHPI	GGTGA	4 cut(s)	346, 850, 1387, 2720
AsuII	TTCGAA	1 cut(s)	2290
AvaI	CYCGRG	5 cut(s)	212, 804, 1623, 2138, 2169
AvaII	GGWCC	5 cut(s)	1008, 1108, 1575, 2362, 2632
BaeI	ACNNNNGTAYC	2 cut(s)	1883, 1916
BamHI	GGATCC	3 cut(s)	456, 1261, 2046
BanI	GGYRCC	1 cut(s)	1185
BanII	GRGCYC	3 cut(s)	219, 1462, 2139
BauI	CACGAG	1 cut(s)	2240
BbsI	GAAGAC	5 cut(s)	629, 1132, 1259, 2117, 2571
Bbv12I	GWGCWC	5 cut(s)	219, 394, 2139, 2170, 2728
BbvI	GCAGC	7 cut(s)	415, 418, 1507, 2042, 2419, 2539, 2626
BccI	CCATC	6 cut(s)	149, 904, 1119, 1520, 2528, 2699
BceAI	ACGGC	4 cut(s)	140, 693, 1352, 1477
BcgI	CGANNNNNNTGC	2 cut(s)	857, 891
BciT130I	CCWGG	4 cut(s)	358, 1244, 1463, 1604
BclI	TGATCA	1 cut(s)	598
BcnI	CCSGG	2 cut(s)	171, 380
BcoDI	GTCTC	3 cut(s)	1150, 2096, 2691
BfaI	CTAG	4 cut(s)	1101, 1404, 1613, 1937
BfmI	CTRYAG	2 cut(s)	1482, 2317
BfoI	RGCGCY	4 cut(s)	97, 136, 211, 386
BglI	GCCNNNNNGGC	1 cut(s)	161
BlpI	GCTNAGC	3 cut(s)	1032, 1311, 2133
Bme1390I	CCNGG	6 cut(s)	171, 358, 380, 1244, 1463, 1604
Bme18I	GGWCC	5 cut(s)	1008, 1108, 1575, 2362, 2632
BmeRI	GACNNNNNGTC	1 cut(s)	2118
BmeT110I	CYCGRG	5 cut(s)	212, 804, 1623, 2138, 2169
BmgT120I	GGNCC	6 cut(s)	163, 1008, 1108, 1575, 2362, 2632
BmiI	GGNNCC	8 cut(s)	458, 1187, 1263, 1553, 1875, 2048, 2454, 2634
BmrFI	CCNGG	6 cut(s)	171, 358, 380, 1244, 1463, 1604
BmsI	GCATC	9 cut(s)	291, 691, 859, 1711, 1849, 1873, 2198, 2206, 2343
BpiI	GAAGAC	5 cut(s)	629, 1132, 1259, 2117, 2571
BpmI	CTGGAG	2 cut(s)	1940, 2430
Bpu1102I	GCTNAGC	3 cut(s)	1032, 1311, 2133
Bpu14I	TTCGAA	1 cut(s)	2290
BpuEI	CTTGAG	5 cut(s)	65, 1058, 1585, 2459, 2750
BpuMI	CCSGG	2 cut(s)	171, 380
BsaBI	GATNNNNATC	2 cut(s)	461, 1530
BsaHI	GRCGYC	2 cut(s)	117, 758
BsaI	GGTCTC	1 cut(s)	2096
BsaJI	CCNNGG	9 cut(s)	169, 305, 582, 675, 1054, 1242, 1461, 1603, 2719
BsaWI	WCCGGW	4 cut(s)	414, 486, 1005, 1188
BsaXI	ACNNNNNCTCC	2 cut(s)	2166, 2196
Bsc4I	CCNNNNNNNGG	6 cut(s)	276, 421, 477, 1117, 2058, 2442
Bse118I	RCCGGY	1 cut(s)	397
Bse1I	ACTGG	2 cut(s)	1140, 1538
Bse3DI	GCAATG	2 cut(s)	1187, 1688
Bse8I	GATNNNNATC	2 cut(s)	461, 1530
BseAI	TCCGGA	1 cut(s)	1005
BseBI	CCWGG	4 cut(s)	358, 1244, 1463, 1604
BseDI	CCNNGG	9 cut(s)	169, 305, 582, 675, 1054, 1242, 1461, 1603, 2719
BseGI	GGATG	5 cut(s)	706, 814, 1864, 2221, 2710
BseJI	GATNNNNATC	2 cut(s)	461, 1530
BseLI	CCNNNNNNNGG	6 cut(s)	276, 421, 477, 1117, 2058, 2442
BseMI	GCAATG	2 cut(s)	1187, 1688
BseMII	CTCAG	5 cut(s)	540, 1023, 2025, 2124, 2334
BseNI	ACTGG	2 cut(s)	1140, 1538
BsePI	GCGCGC	1 cut(s)	151
BseXI	GCAGC	7 cut(s)	415, 418, 1507, 2042, 2419, 2539, 2626
BseYI	CCCAGC	1 cut(s)	2635
BsgI	GTGCAG	2 cut(s)	422, 2049
Bsh1236I	CGCG	3 cut(s)	153, 204, 307
Bsh1285I	CGRYCG	2 cut(s)	415, 2213
BshFI	GGCC	3 cut(s)	164, 581, 1367
BshNI	GGYRCC	1 cut(s)	1185
BsiEI	CGRYCG	2 cut(s)	415, 2213
BsiHKAI	GWGCWC	5 cut(s)	219, 394, 2139, 2170, 2728
BsiHKCI	CYCGRG	5 cut(s)	212, 804, 1623, 2138, 2169
BsiSI	CCGG	8 cut(s)	171, 367, 379, 398, 415, 487, 1006, 1189
BslFI	GGGAC	2 cut(s)	2399, 2618
BslI	CCNNNNNNNGG	6 cut(s)	276, 421, 477, 1117, 2058, 2442
BsmAI	GTCTC	3 cut(s)	1150, 2096, 2691
BsmFI	GGGAC	2 cut(s)	2399, 2618
BsmI	GAATGC	2 cut(s)	676, 1724
BsnI	GGCC	3 cut(s)	164, 581, 1367
Bso31I	GGTCTC	1 cut(s)	2096
BsoBI	CYCGRG	5 cut(s)	212, 804, 1623, 2138, 2169
Bsp119I	TTCGAA	1 cut(s)	2290
Bsp1286I	GDGCHC	6 cut(s)	219, 394, 1462, 2139, 2170, 2728
Bsp13I	TCCGGA	1 cut(s)	1005
Bsp1720I	GCTNAGC	3 cut(s)	1032, 1311, 2133
Bsp19I	CCATGG	1 cut(s)	1054
BspANI	GGCC	3 cut(s)	164, 581, 1367
BspCNI	CTCAG	5 cut(s)	541, 1024, 2026, 2125, 2335
BspEI	TCCGGA	1 cut(s)	1005
BspFNI	CGCG	3 cut(s)	153, 204, 307
BspLI	GGNNCC	8 cut(s)	458, 1187, 1263, 1553, 1875, 2048, 2454, 2634
BspPI	GGATC	8 cut(s)	105, 451, 464, 1256, 1269, 2041, 2054, 2198
BspQI	GCTCTTC	2 cut(s)	48, 2138
BspT104I	TTCGAA	1 cut(s)	2290
BspT107I	GGYRCC	1 cut(s)	1185
BspTNI	GGTCTC	1 cut(s)	2096
BsrBI	CCGCTC	1 cut(s)	574
BsrDI	GCAATG	2 cut(s)	1187, 1688
BsrFI	RCCGGY	1 cut(s)	397
BsrI	ACTGG	2 cut(s)	1140, 1538
BssAI	RCCGGY	1 cut(s)	397
BssECI	CCNNGG	9 cut(s)	169, 305, 582, 675, 1054, 1242, 1461, 1603, 2719
BssHII	GCGCGC	1 cut(s)	151
BssNI	GRCGYC	2 cut(s)	117, 758
BssSI	CACGAG	1 cut(s)	2240
BssT1I	CCWWGG	2 cut(s)	1054, 2719
Bst2BI	CACGAG	1 cut(s)	2240
Bst2UI	CCWGG	4 cut(s)	358, 1244, 1463, 1604
Bst4CI	ACNGT	4 cut(s)	835, 844, 1258, 1390
Bst6I	CTCTTC	5 cut(s)	48, 882, 981, 1507, 2138
BstACI	GRCGYC	2 cut(s)	117, 758
BstAPI	GCANNNNNTGC	1 cut(s)	301
BstBI	TTCGAA	1 cut(s)	2290
BstC8I	GCNNGC	8 cut(s)	153, 206, 579, 900, 1272, 1947, 2076, 2314
BstDEI	CTNAG	8 cut(s)	549, 666, 1032, 1311, 1974, 2034, 2133, 2343
BstDSI	CCRYGG	3 cut(s)	305, 675, 1054
BstEII	GGTNACC	1 cut(s)	352
BstENI	CCTNNNNNAGG	1 cut(s)	274
BstF5I	GGATG	5 cut(s)	706, 814, 1864, 2221, 2710
BstFNI	CGCG	3 cut(s)	153, 204, 307
BstH2I	RGCGCY	4 cut(s)	97, 136, 211, 386
BstHHI	GCGC	7 cut(s)	96, 135, 153, 155, 210, 385, 880
BstMAI	GTCTC	3 cut(s)	1150, 2096, 2691
BstMCI	CGRYCG	2 cut(s)	415, 2213
BstNI	CCWGG	4 cut(s)	358, 1244, 1463, 1604
BstNSI	RCATGY	2 cut(s)	1772, 2008
BstPI	GGTNACC	1 cut(s)	352
BstSCI	CCNGG	6 cut(s)	169, 356, 378, 1242, 1461, 1602
BstSFI	CTRYAG	2 cut(s)	1482, 2317
BstUI	CGCG	3 cut(s)	153, 204, 307
BstV1I	GCAGC	7 cut(s)	415, 418, 1507, 2042, 2419, 2539, 2626
BstV2I	GAAGAC	5 cut(s)	629, 1132, 1259, 2117, 2571
BstX2I	RGATCY	4 cut(s)	456, 1261, 2046, 2203
BstYI	RGATCY	4 cut(s)	456, 1261, 2046, 2203
BsuRI	GGCC	3 cut(s)	164, 581, 1367
BtgI	CCRYGG	3 cut(s)	305, 675, 1054
BtsCI	GGATG	5 cut(s)	706, 814, 1864, 2221, 2710
BtsI	GCAGTG	2 cut(s)	1281, 1635
BtsIMutI	CAGTG	5 cut(s)	862, 1263, 1281, 1395, 1635
Cac8I	GCNNGC	8 cut(s)	153, 206, 579, 900, 1272, 1947, 2076, 2314
CaiI	CAGNNNCTG	2 cut(s)	334, 2642
CfoI	GCGC	7 cut(s)	96, 135, 153, 155, 210, 385, 880
Cfr10I	RCCGGY	1 cut(s)	397
Cfr13I	GGNCC	6 cut(s)	163, 1008, 1108, 1575, 2362, 2632
Cfr42I	CCGCGG	1 cut(s)	308
CseI	GACGC	1 cut(s)	125
Csp6I	GTAC	6 cut(s)	190, 250, 493, 1608, 1746, 1762
CviAII	CATG	8 cut(s)	515, 1055, 1070, 1328, 1769, 1991, 2005, 2258
CviQI	GTAC	6 cut(s)	190, 250, 493, 1608, 1746, 1762
DdeI	CTNAG	8 cut(s)	549, 666, 1032, 1311, 1974, 2034, 2133, 2343
DraI	TTTAAA	1 cut(s)	1453
DriI	GACNNNNNGTC	1 cut(s)	2118
Eam1104I	CTCTTC	5 cut(s)	48, 882, 981, 1507, 2138
Eam1105I	GACNNNNNGTC	1 cut(s)	2118
EarI	CTCTTC	5 cut(s)	48, 882, 981, 1507, 2138
EciI	GGCGGA	3 cut(s)	584, 782, 2709
Ecl136II	GAGCTC	2 cut(s)	217, 2137
Eco130I	CCWWGG	2 cut(s)	1054, 2719
Eco24I	GRGCYC	3 cut(s)	219, 1462, 2139
Eco31I	GGTCTC	1 cut(s)	2096
Eco47I	GGWCC	5 cut(s)	1008, 1108, 1575, 2362, 2632
Eco53kI	GAGCTC	2 cut(s)	217, 2137
Eco57I	CTGAAG	2 cut(s)	2616, 2700
Eco88I	CYCGRG	5 cut(s)	212, 804, 1623, 2138, 2169
Eco91I	GGTNACC	1 cut(s)	352
EcoICRI	GAGCTC	2 cut(s)	217, 2137
EcoNI	CCTNNNNNAGG	1 cut(s)	274
EcoO109I	RGGNCCY	2 cut(s)	163, 2632
EcoO65I	GGTNACC	1 cut(s)	352
EcoRI	GAATTC	1 cut(s)	2222
EcoRII	CCWGG	4 cut(s)	356, 1242, 1461, 1602
EcoT14I	CCWWGG	2 cut(s)	1054, 2719
EcoT22I	ATGCAT	2 cut(s)	1690, 1726
EcoT38I	GRGCYC	3 cut(s)	219, 1462, 2139
ErhI	CCWWGG	2 cut(s)	1054, 2719
FaeI	CATG	8 cut(s)	518, 1058, 1073, 1331, 1772, 1994, 2008, 2261
FalI	AAGNNNNNCTT	2 cut(s)	42, 74
FaqI	GGGAC	2 cut(s)	2399, 2618
FatI	CATG	8 cut(s)	514, 1054, 1069, 1327, 1768, 1990, 2004, 2257
FauI	CCCGC	2 cut(s)	1773, 1938
FauNDI	CATATG	1 cut(s)	1781
FbaI	TGATCA	1 cut(s)	598
FblI	GTMKAC	3 cut(s)	483, 1017, 1619
FokI	GGATG	5 cut(s)	713, 821, 1871, 2228, 2717
FriOI	GRGCYC	3 cut(s)	219, 1462, 2139
FspBI	CTAG	4 cut(s)	1101, 1404, 1613, 1937
FspI	TGCGCA	1 cut(s)	879
GlaI	GCGC	7 cut(s)	95, 134, 152, 154, 209, 384, 879
GsaI	CCCAGC	1 cut(s)	2639
GsuI	CTGGAG	2 cut(s)	1940, 2430
HaeII	RGCGCY	4 cut(s)	97, 136, 211, 386
HaeIII	GGCC	3 cut(s)	164, 581, 1367
HapII	CCGG	8 cut(s)	171, 367, 379, 398, 415, 487, 1006, 1189
HgaI	GACGC	1 cut(s)	125
HhaI	GCGC	7 cut(s)	96, 135, 153, 155, 210, 385, 880
Hin1I	GRCGYC	2 cut(s)	117, 758
Hin1II	CATG	8 cut(s)	518, 1058, 1073, 1331, 1772, 1994, 2008, 2261
Hin6I	GCGC	7 cut(s)	94, 133, 151, 153, 208, 383, 878
HinP1I	GCGC	7 cut(s)	94, 133, 151, 153, 208, 383, 878
HpaII	CCGG	8 cut(s)	171, 367, 379, 398, 415, 487, 1006, 1189
HphI	GGTGA	4 cut(s)	346, 850, 1387, 2720
Hpy166II	GTNNAC	8 cut(s)	484, 732, 756, 1018, 1378, 1588, 1610, 1620
Hpy8I	GTNNAC	8 cut(s)	484, 732, 756, 1018, 1378, 1588, 1610, 1620
Hpy99I	CGWCG	2 cut(s)	119, 763
HpyAV	CCTTC	6 cut(s)	133, 280, 445, 793, 944, 1948
HpyCH4III	ACNGT	4 cut(s)	835, 844, 1258, 1390
HpyCH4IV	ACGT	4 cut(s)	758, 1292, 1837, 2273
HpyF3I	CTNAG	8 cut(s)	549, 666, 1032, 1311, 1974, 2034, 2133, 2343
HpySE526I	ACGT	4 cut(s)	758, 1292, 1837, 2273
Hsp92I	GRCGYC	2 cut(s)	117, 758
Hsp92II	CATG	8 cut(s)	518, 1058, 1073, 1331, 1772, 1994, 2008, 2261
HspAI	GCGC	7 cut(s)	94, 133, 151, 153, 208, 383, 878
Kpn2I	TCCGGA	1 cut(s)	1005
Ksp22I	TGATCA	1 cut(s)	598
KspI	CCGCGG	1 cut(s)	308
LguI	GCTCTTC	2 cut(s)	48, 2138
LmnI	GCTCC	6 cut(s)	340, 424, 571, 1557, 1879, 2458
Lsp1109I	GCAGC	7 cut(s)	415, 418, 1507, 2042, 2419, 2539, 2626
LweI	GCATC	9 cut(s)	291, 691, 859, 1711, 1849, 1873, 2198, 2206, 2343
MaeI	CTAG	4 cut(s)	1101, 1404, 1613, 1937
MaeII	ACGT	4 cut(s)	758, 1292, 1837, 2273
MaeIII	GTNAC	3 cut(s)	286, 352, 838
MbiI	CCGCTC	1 cut(s)	574
MflI	RGATCY	4 cut(s)	456, 1261, 2046, 2203
MhlI	GDGCHC	6 cut(s)	219, 394, 1462, 2139, 2170, 2728
MlyI	GAGTC	5 cut(s)	355, 505, 554, 1625, 2421
MmeI	TCCRAC	2 cut(s)	138, 624
Mph1103I	ATGCAT	2 cut(s)	1690, 1726
MroI	TCCGGA	1 cut(s)	1005
MroXI	GAANNNNTTC	3 cut(s)	57, 2286, 2674
MseI	TTAA	7 cut(s)	591, 615, 723, 780, 906, 1452, 1866
MslI	CAYNNNNRTG	1 cut(s)	2262
MspA1I	CMGCKG	3 cut(s)	307, 2033, 2639
MspI	CCGG	8 cut(s)	171, 367, 379, 398, 415, 487, 1006, 1189
MspR9I	CCNGG	6 cut(s)	171, 358, 380, 1244, 1463, 1604
Mva1269I	GAATGC	2 cut(s)	676, 1724
MvaI	CCWGG	4 cut(s)	358, 1244, 1463, 1604
MvnI	CGCG	3 cut(s)	153, 204, 307
NciI	CCSGG	2 cut(s)	171, 380
NcoI	CCATGG	1 cut(s)	1054
NdeI	CATATG	1 cut(s)	1781
NlaIII	CATG	8 cut(s)	518, 1058, 1073, 1331, 1772, 1994, 2008, 2261
NlaIV	GGNNCC	8 cut(s)	458, 1187, 1263, 1553, 1875, 2048, 2454, 2634
NmuCI	GTSAC	3 cut(s)	286, 352, 838
NsbI	TGCGCA	1 cut(s)	879
NsiI	ATGCAT	2 cut(s)	1690, 1726
NspI	RCATGY	2 cut(s)	1772, 2008
NspV	TTCGAA	1 cut(s)	2290
PaeR7I	CTCGAG	2 cut(s)	212, 2138
PauI	GCGCGC	1 cut(s)	151
PciSI	GCTCTTC	2 cut(s)	48, 2138
PcsI	WCGNNNNNNNCGW	2 cut(s)	111, 153
PctI	GAATGC	2 cut(s)	676, 1724
PdmI	GAANNNNTTC	3 cut(s)	57, 2286, 2674
PfeI	GAWTC	8 cut(s)	316, 1213, 1531, 2084, 2292, 2522, 2591, 2675
PflMI	CCANNNNNTGG	1 cut(s)	1117
Ple19I	CGATCG	1 cut(s)	2213
PleI	GAGTC	5 cut(s)	355, 505, 553, 1624, 2420
PpsI	GAGTC	5 cut(s)	355, 505, 553, 1624, 2420
PpuMI	RGGWCCY	1 cut(s)	2632
Psp124BI	GAGCTC	2 cut(s)	219, 2139
Psp5II	RGGWCCY	1 cut(s)	2632
Psp6I	CCWGG	4 cut(s)	356, 1242, 1461, 1602
PspEI	GGTNACC	1 cut(s)	352
PspFI	CCCAGC	1 cut(s)	2635
PspGI	CCWGG	4 cut(s)	356, 1242, 1461, 1602
PspN4I	GGNNCC	8 cut(s)	458, 1187, 1263, 1553, 1875, 2048, 2454, 2634
PspPI	GGNCC	6 cut(s)	163, 1008, 1108, 1575, 2362, 2632
PspPPI	RGGWCCY	1 cut(s)	2632
PspXI	VCTCGAGB	1 cut(s)	212
PsrI	GAACNNNNNNTAC	2 cut(s)	422, 454
PstNI	CAGNNNCTG	2 cut(s)	334, 2642
PsuI	RGATCY	4 cut(s)	456, 1261, 2046, 2203
PteI	GCGCGC	1 cut(s)	151
PvuI	CGATCG	1 cut(s)	2213
PvuII	CAGCTG	2 cut(s)	2033, 2639
RsaI	GTAC	6 cut(s)	191, 251, 494, 1609, 1747, 1763
RsaNI	GTAC	6 cut(s)	190, 250, 493, 1608, 1746, 1762
RseI	CAYNNNNRTG	1 cut(s)	2262
SacI	GAGCTC	2 cut(s)	219, 2139
SacII	CCGCGG	1 cut(s)	308
SapI	GCTCTTC	2 cut(s)	48, 2138
SaqAI	TTAA	7 cut(s)	591, 615, 723, 780, 906, 1452, 1866
Sau96I	GGNCC	6 cut(s)	163, 1008, 1108, 1575, 2362, 2632
SchI	GAGTC	5 cut(s)	355, 505, 554, 1625, 2421
ScrFI	CCNGG	6 cut(s)	171, 358, 380, 1244, 1463, 1604
SduI	GDGCHC	6 cut(s)	219, 394, 1462, 2139, 2170, 2728
SfaNI	GCATC	9 cut(s)	291, 691, 859, 1711, 1849, 1873, 2198, 2206, 2343
SfcI	CTRYAG	2 cut(s)	1482, 2317
Sfr274I	CTCGAG	2 cut(s)	212, 2138
Sfr303I	CCGCGG	1 cut(s)	308
SfuI	TTCGAA	1 cut(s)	2290
SgrBI	CCGCGG	1 cut(s)	308
SinI	GGWCC	5 cut(s)	1008, 1108, 1575, 2362, 2632
SlaI	CTCGAG	2 cut(s)	212, 2138
SmiMI	CAYNNNNRTG	1 cut(s)	2262
SmlI	CTYRAG	8 cut(s)	12, 80, 212, 1037, 1564, 2138, 2438, 2729
SmoI	CTYRAG	8 cut(s)	12, 80, 212, 1037, 1564, 2138, 2438, 2729
SspI	AATATT	1 cut(s)	1755
SspMI	CTAG	4 cut(s)	1101, 1404, 1613, 1937
SstI	GAGCTC	2 cut(s)	219, 2139
StyD4I	CCNGG	6 cut(s)	169, 356, 378, 1242, 1461, 1602
StyI	CCWWGG	2 cut(s)	1054, 2719
TaaI	ACNGT	4 cut(s)	835, 844, 1258, 1390
TaiI	ACGT	4 cut(s)	761, 1295, 1840, 2276
TaqI	TCGA	6 cut(s)	213, 273, 867, 1833, 2139, 2290
TauI	GCSGC	7 cut(s)	138, 307, 569, 577, 1441, 1874, 2718
TfiI	GAWTC	8 cut(s)	316, 1213, 1531, 2084, 2292, 2522, 2591, 2675
Tru1I	TTAA	7 cut(s)	591, 615, 723, 780, 906, 1452, 1866
Tru9I	TTAA	7 cut(s)	591, 615, 723, 780, 906, 1452, 1866
TscAI	CASTG	5 cut(s)	862, 1263, 1281, 1395, 1635
TseFI	GTSAC	3 cut(s)	286, 352, 838
TseI	GCWGC	7 cut(s)	403, 406, 1520, 2030, 2432, 2552, 2639
Tsp45I	GTSAC	3 cut(s)	286, 352, 838
TspDTI	ATGAA	8 cut(s)	1335, 1752, 1967, 1980, 2007, 2025, 2280, 2439
TspGWI	ACGGA	2 cut(s)	353, 2314
TspRI	CASTG	5 cut(s)	862, 1263, 1281, 1395, 1635
Van91I	CCANNNNNTGG	1 cut(s)	1117
VpaK11BI	GGWCC	5 cut(s)	1008, 1108, 1575, 2362, 2632
XagI	CCTNNNNNAGG	1 cut(s)	274
XapI	RAATTY	3 cut(s)	1447, 1802, 2222
XceI	RCATGY	2 cut(s)	1772, 2008
XhoI	CTCGAG	2 cut(s)	212, 2138
XmiI	GTMKAC	3 cut(s)	483, 1017, 1619
XmnI	GAANNNNTTC	3 cut(s)	57, 2286, 2674
XspI	CTAG	4 cut(s)	1101, 1404, 1613, 1937
ZraI	GACGTC	1 cut(s)	759
Zsp2I	ATGCAT	2 cut(s)	1690, 1726

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.