Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr1

Orthogroup ID

OG0001333

Physical Location & Seq

Genomic Coordinates Reverse (-)

57398402 .. 57401131

Gene Structure 2730 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000004227

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2544 bp

ATGGCTATCTCTCAAGAACTCTACCCCTCCCAAGACGACCTCCTCTACGAAGAAGAGCTTCTCCGAAACCCTTACAGCCTCAAGCTCTGGTGGCGCTACCGTATCGCTATATCCGACGCGCCCTTCAAGAAGCGCCGCACCATCTACGAGCGCACCCTCAAGGACCTTTCCGGAAGCTACAAGCTTTGGTACGCCTACCTCCGCGAGCGTCTAGAGCTCTTACGCAACTTTCCGATCAACCACTCCGAGTACGAAACCCTAAACAACACCTTCGAGAGGGCGTTGGTCACAATGCATAAGATGCCGCGGATTTGGATATCGTATTTACAGAGCCTGACGGAGCAGAGATTGGTCACGAGGACTCGCCGGAGTTTCGATCTGGAGCTGTGTGCTCTGCCGAAGGGGATCCCTATCCAGACCTCGCTTCGGGTCTACCGGAGGTACTTGCTTTATGACCCGACTCATGTTGAGGATTTTATTGCGTTTTTGATAGAGTCTGAGCTGTGGCAAGAGGCGTCGGCGCGGCTGGCCTCGGTGCTTAATGATGATCAGTTTAGGTCGATTAAGGGAAAGACTAAGCATAGGTTGTGGTTGGAGTTGTGTGCTTTGCTTACGAAGAATGCCACGGCGGTTTCGTGGTTGAAAGTGGATGCTATCATTAGAGGTGGGATTAAGAAGTTTACGTATGAGGTGGGAAGGCTGTGGACGTCGTTGCCGGATTATTATATTAAAAGGGGTTTGTTTGAGAAGGCTAGGGATGTGTTTGAGGAGGGGATGCAGACTGTGGTGACTGTGAGGGATTTTAGTGTGATTTTTTCTGCGTATGCGCAGTTTGAAGAGAGTATGCTTGCAATTAAGATGGAGGCTTTGGGGAGTGATGAGGAGGAGGAGAAGAAAGGAGAGAATGGGAGAATGGAAGATGAGGGCAGTGAAGACGAGGAGGAAGATGTCGGAACCAATGTAGACTTGTCTCTGGCTGAGCTTGAGAGGAATATACTCCATGGGTTTTGGCTACATGATGAGAATGGTGTTGATTTGAGGTTGGCTCGATTGGACCGTCTTATGGATAGAAGACCTGAACTGGCTAATAGTGTTCTTTTGAGACAAAATCCGCATAATGTAGAGCAATGGCACCAGAGAGTGAAGCTGTTTGAGGGGAATCCTACAAAGCAGATACTTACTTACACCCAGGCTGTGAAGACAGTGGACCCAATGCAAGCAGTGGGGAAGCCTCATACGTTGTGGGTTGCTTTTGCTAAGCTGTATGAAACACATGAAGATATTGCAAATGCAAGAGTAATTTTTGATAAGGCCGTCCAGCTATGGTGCGAGTGGGCAGAAATGGAATTGCGGCATAAGAATTTTAGACGCGCCCTGGAACTGATGAGGAGAGCTACAGCAGAGCCGTCTGTTGAGGTTAAACGAAGAGTTGCTGCTGATGGGAATCCACCAGTTCAGATGAGGCTCCATAAATCCTTGAGGCTTTGGACCTTTTATGTGGACTTGGAGGAGAGCCTGGGCACACTAGAGTCTACTCGGGCAGTGTATGAGAGGATATTGGATTTGAGAATAGCCACACCTCAAATAATAATCAACTATGCATTGCTTCTGGAAGAACATAAATACTTTGAAGATGCATTTAAAGTCTATGAAAAGGGTACTCAAATATTCAAGTACCCGCATGTCAAAGACATATGGATTATATATCTCTCTAAACTTGTAAAAAGATATGGGAAGAATAAGCTGGAACGTGCAAGACTGCTCTTTGAGGATGCTGTTAAAGCGGCTCCTGCTGATGCGAAGAAATCACTGTATCTTCAATTTGCAAAGCTGGAGGAGGATTATGGTCTAGCAAAGCGGGCTATGAAGGTCTATGATGAAGCAACTAAGGCTGTTCCAAACCATGAAAAGTTGAGCATGTATGAAATCTATATTGCACGTGCAGCTGAGATATTTGGGATCCCCAAAACAAGGGAGATATATGAGCAAACAATAGAATCTGGACTCCCTGACAAAGATGTGAAGACAATGTGTCTAAAGTATGCAGAGCTCGAGAAGAGCTTGGGCGAAATTGATCGTGCTCGAGGAGTTTACGTATTTGCATCTCAGTTTTCAGATCCACGATCGGATGCAGAATTTTGGAACAAATGGCACGAGTTTGAGGATCAGAGGTTGAGCATTGACGAGGCAAAAGACAAATTGAAACAGGCTGGAGTCCCCAAAGATGAAATGGCTGCTCTTGAGAGGCAGTTGGCTCCTGCGATCAGCGATACACCCTCTAAAGACAGCAACAGAAAAGTGGGCTTTGTGAGTGCTGGAATAGAATCACAGATTAATGGAGGGATTGCAGCAACTTTTAATCATGAAGACATTGAACTACCAGAAGAAAGTGATTATGAAGATGAGGCAAATGTTGAAATAGCACAAAAGGAGGTCCCCGCTGCTGTTTTTGGAGATTTGGCTAACAAGAGAAAGAGGATTCTGAAGATAATGAGGGCGGAGACAAGGATGGTGAGAGCCGCCTTGGTGCTCTTGAGAGAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000349 GO:0000375 GO:0000377 GO:0000393 GO:0000398 GO:0000974 GO:0001701 GO:0001824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005681 GO:0005684 GO:0006139 GO:0006259 GO:0006281 GO:0006283 GO:0006289 GO:0006351 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007275 GO:0008150 GO:0008152 GO:0008380 GO:0009058 GO:0009059 GO:0009790 GO:0009792 GO:0009987 GO:0010467 GO:0016043 GO:0016070 GO:0016071 GO:0018130 GO:0019438 GO:0022607 GO:0022613 GO:0022618 GO:0032501 GO:0032502 GO:0032774 GO:0032991 GO:0033554 GO:0034622 GO:0034641 GO:0034645 GO:0034654 GO:0043009 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0048856 GO:0050896 GO:0051716 GO:0065003 GO:0071007 GO:0071010 GO:0071012 GO:0071013 GO:0071014 GO:0071704 GO:0071826 GO:0071840 GO:0090304 GO:0097659 GO:1901360 GO:1901362 GO:1901576 GO:1902494 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko03040 map03040

Pfam Domains

Protein Families

Suf

Protein Analysis

848

Amino Acids

98.75

Weight (kDa)

6.07

Isoelectric Point (pI)

44.53

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HAT_Syf1_CNRKL1_N	PF23233	12 - 133	7.7e-39	Pre-mRNA-splicing factor Syf1/CNRKL1 N-terminal HAT repeat
HAT_Syf1_M	PF23220	156 - 302	7e-54	Pre-mRNA-splicing factor SYF1 middle HAT repeat
HAT_Syf1_M	PF23220	339 - 402	9.8e-23	Pre-mRNA-splicing factor SYF1 middle HAT repeat
Suf	PF05843	372 - 639	3.7e-09	Suppressor of forked protein (Suf)
HAT_Syf1_CNRKL1_C	PF23231	404 - 732	2.5e-155	Pre-mRNA-splicing factor Syf1/CRNKL1 C-terminal HAT repeat
HAT_PRP39_C	PF23241	491 - 557	6.6e-06	PRP39 C-terminal HAT repeat
HAT_PRP39_N	PF23240	532 - 630	5.7e-07	PRP39 N-terminal HAT repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001333)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G28740
fragaria_vesca	FvH4_3g03910 FvH4_3g04150 FvH4_3g04150 FvH4_3g04150 FvH4_3g04150
malus_domestica	MD05G1324400.v1.1 MD10G1302200.v1.1
prunus_persica	Prupe.5G013200_v2.0.a1
pyrus_communis	pycom05g29860 pycom10g25470
rosa_chinensis	RchiOBHm_Chr5g0006181 RchiOBHm_Chr5g0006191 RchiOBHm_Chr5g0006201 RchiOBHm_Chr5g0006601 RchiOBHm_Chr7g0193971
rosa_laevigata	RLG00000004227 RLG00000031375 RLG00000031376 RLG00000031403
rosa_multiflora	Rmu_co7988368.1_g000001 Rmu_co8257165.1_g000001 Rmu_co8304153.1_g000001 Rmu_co8327759.1_g000001 Rmu_sc0001972.1_g000002 Rmu_sc0002389.1_g000006 Rmu_sc0004331.1_g000002 Rmu_sc0005583.1_g000001 Rmu_sc0009371.1_g000007 Rmu_sc0011154.1_g000005 Rmu_sc0024345.1_g000002 Rmu_sc0032392.1_g000001 Rmu_sc0032393.1_g000001
rosa_roxburghii	Rroxscaffold_1G00069420 Rroxscaffold_1G00069440 Rroxscaffold_1G00069800 Rroxscaffold_2G00122720 Rroxscaffold_3G00261670
rosa_rugosa	Rorug04G0422900
rosa_samantha	Rh5AG050200 Rh5BG046700 Rh5BG046800 Rh5BG049400 Rh5CG055400 Rh5CG055600 Rh5CG057800 Rh5DG046500 Rh5DG048800 Rh7AG139900 Rh7AG140000 Rh7DG141300
rosa_wichuraiana	Rw5G004790 Rw5G005020 Rw7G011940

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	710
Acc16I	TGCGCA	1 cut(s)	828
AccB1I	GGYRCC	1 cut(s)	1131
AccI	GTMKAC	3 cut(s)	432, 963, 1532
AccII	CGCG	5 cut(s)	119, 204, 307, 523, 1371
AccIII	TCCGGA	1 cut(s)	170
AclWI	GGATC	6 cut(s)	400, 413, 1954, 1967, 2111, 2172
AcsI	RAATTY	2 cut(s)	1360, 2135
AcuI	CTGAAG	1 cut(s)	2504
AcvI	CACGTG	1 cut(s)	1940
AcyI	GRCGYC	2 cut(s)	515, 707
AfaI	GTAC	5 cut(s)	191, 251, 443, 1660, 1676
AfiI	CCNNNNNNNGG	4 cut(s)	276, 426, 1063, 1971
AgsI	TTSAA	9 cut(s)	127, 643, 836, 1631, 1672, 1820, 2203, 2375, 2417
AhdI	GACNNNNNGTC	1 cut(s)	2031
AjnI	CCWGG	3 cut(s)	1188, 1374, 1515
Alw21I	GWGCWC	5 cut(s)	219, 394, 2052, 2083, 2532
Alw26I	GTCTC	3 cut(s)	975, 1096, 2495
AlwI	GGATC	6 cut(s)	400, 413, 1954, 1967, 2111, 2172
AlwNI	CAGNNNCTG	1 cut(s)	334
Ama87I	CYCGRG	3 cut(s)	1536, 2051, 2082
Aor13HI	TCCGGA	1 cut(s)	170
AoxI	GGCC	2 cut(s)	528, 1311
ApeKI	GCWGC	5 cut(s)	1433, 1943, 2234, 2348, 2441
ApoI	RAATTY	2 cut(s)	1360, 2135
AseI	ATTAAT	1 cut(s)	2334
Asp700I	GAANNNNTTC	1 cut(s)	57
AspLEI	GCGC	7 cut(s)	96, 121, 135, 153, 523, 829, 1373
AspS9I	GGNCC	5 cut(s)	163, 1054, 1207, 1488, 2434
AsuHPI	GGTGA	2 cut(s)	799, 2524
AvaI	CYCGRG	3 cut(s)	1536, 2051, 2082
AvaII	GGWCC	5 cut(s)	163, 1054, 1207, 1488, 2434
BaeGI	GKGCMC	1 cut(s)	1523
BamHI	GGATCC	2 cut(s)	405, 1959
BanI	GGYRCC	1 cut(s)	1131
BanII	GRGCYC	2 cut(s)	219, 2052
BauI	CACGAG	2 cut(s)	355, 2153
BbrPI	CACGTG	1 cut(s)	1940
BbsI	GAAGAC	5 cut(s)	939, 1078, 1205, 2030, 2373
Bbv12I	GWGCWC	5 cut(s)	219, 394, 2052, 2083, 2532
BbvI	GCAGC	5 cut(s)	1420, 1955, 2221, 2360, 2428
BccI	CCATC	4 cut(s)	149, 853, 1433, 2503
BceAI	ACGGC	3 cut(s)	642, 1298, 1390
BciT130I	CCWGG	3 cut(s)	1190, 1376, 1517
BclI	TGATCA	1 cut(s)	547
BcoDI	GTCTC	3 cut(s)	975, 1096, 2495
BfaI	CTAG	4 cut(s)	212, 753, 1526, 1850
BfmI	CTRYAG	1 cut(s)	1395
BfoI	RGCGCY	2 cut(s)	97, 136
BlpI	GCTNAGC	2 cut(s)	978, 1257
Bme1390I	CCNGG	3 cut(s)	1190, 1376, 1517
Bme18I	GGWCC	5 cut(s)	163, 1054, 1207, 1488, 2434
BmeRI	GACNNNNNGTC	1 cut(s)	2031
BmeT110I	CYCGRG	3 cut(s)	1536, 2051, 2082
BmgT120I	GGNCC	5 cut(s)	163, 1054, 1207, 1488, 2434
BmiI	GGNNCC	9 cut(s)	407, 955, 1133, 1209, 1466, 1788, 1961, 2256, 2436
BmrFI	CCNGG	3 cut(s)	1190, 1376, 1517
BmsI	GCATC	8 cut(s)	291, 640, 765, 1624, 1762, 1786, 2111, 2119
BpiI	GAAGAC	5 cut(s)	939, 1078, 1205, 2030, 2373
BplI	GAGNNNNNCTC	2 cut(s)	1030, 1062
BpmI	CTGGAG	3 cut(s)	401, 1853, 2232
Bpu1102I	GCTNAGC	2 cut(s)	978, 1257
BpuEI	CTTGAG	5 cut(s)	65, 143, 1004, 1498, 2261
BsaAI	YACGTR	3 cut(s)	684, 1940, 2095
BsaBI	GATNNNNATC	2 cut(s)	410, 1443
BsaHI	GRCGYC	2 cut(s)	515, 707
BsaJI	CCNNGG	8 cut(s)	305, 531, 624, 1000, 1188, 1374, 1516, 2523
BsaWI	WCCGGW	2 cut(s)	170, 435
BsaXI	ACNNNNNCTCC	2 cut(s)	2079, 2109
Bsc4I	CCNNNNNNNGG	4 cut(s)	276, 426, 1063, 1971
Bse1I	ACTGG	2 cut(s)	1086, 1451
Bse3DI	GCAATG	2 cut(s)	1133, 1601
Bse8I	GATNNNNATC	2 cut(s)	410, 1443
BseAI	TCCGGA	1 cut(s)	170
BseBI	CCWGG	3 cut(s)	1190, 1376, 1517
BseDI	CCNNGG	8 cut(s)	305, 531, 624, 1000, 1188, 1374, 1516, 2523
BseGI	GGATG	6 cut(s)	655, 763, 780, 1777, 2134, 2514
BseJI	GATNNNNATC	2 cut(s)	410, 1443
BseLI	CCNNNNNNNGG	4 cut(s)	276, 426, 1063, 1971
BseMI	GCAATG	2 cut(s)	1133, 1601
BseMII	CTCAG	4 cut(s)	489, 969, 1938, 2120
BseNI	ACTGG	2 cut(s)	1086, 1451
BseSI	GKGCMC	1 cut(s)	1523
BseXI	GCAGC	5 cut(s)	1420, 1955, 2221, 2360, 2428
BsgI	GTGCAG	1 cut(s)	1962
Bsh1236I	CGCG	5 cut(s)	119, 204, 307, 523, 1371
Bsh1285I	CGRYCG	1 cut(s)	2126
BshFI	GGCC	2 cut(s)	530, 1313
BshNI	GGYRCC	1 cut(s)	1131
BsiEI	CGRYCG	1 cut(s)	2126
BsiHKAI	GWGCWC	5 cut(s)	219, 394, 2052, 2083, 2532
BsiHKCI	CYCGRG	3 cut(s)	1536, 2051, 2082
BsiSI	CCGG	4 cut(s)	171, 367, 436, 716
BslFI	GGGAC	2 cut(s)	2201, 2420
BslI	CCNNNNNNNGG	4 cut(s)	276, 426, 1063, 1971
BsmAI	GTCTC	3 cut(s)	975, 1096, 2495
BsmFI	GGGAC	2 cut(s)	2201, 2420
BsmI	GAATGC	1 cut(s)	625
BsnI	GGCC	2 cut(s)	530, 1313
BsoBI	CYCGRG	3 cut(s)	1536, 2051, 2082
Bsp1286I	GDGCHC	6 cut(s)	219, 394, 1523, 2052, 2083, 2532
Bsp13I	TCCGGA	1 cut(s)	170
Bsp1720I	GCTNAGC	2 cut(s)	978, 1257
Bsp19I	CCATGG	1 cut(s)	1000
BspANI	GGCC	2 cut(s)	530, 1313
BspCNI	CTCAG	4 cut(s)	490, 970, 1939, 2119
BspEI	TCCGGA	1 cut(s)	170
BspFNI	CGCG	5 cut(s)	119, 204, 307, 523, 1371
BspHI	TCATGA	1 cut(s)	2362
BspLI	GGNNCC	9 cut(s)	407, 955, 1133, 1209, 1466, 1788, 1961, 2256, 2436
BspPI	GGATC	6 cut(s)	400, 413, 1954, 1967, 2111, 2172
BspQI	GCTCTTC	2 cut(s)	48, 2051
BspT107I	GGYRCC	1 cut(s)	1131
BsrDI	GCAATG	2 cut(s)	1133, 1601
BsrI	ACTGG	2 cut(s)	1086, 1451
BssECI	CCNNGG	8 cut(s)	305, 531, 624, 1000, 1188, 1374, 1516, 2523
BssNI	GRCGYC	2 cut(s)	515, 707
BssSI	CACGAG	2 cut(s)	355, 2153
BssT1I	CCWWGG	2 cut(s)	1000, 2523
Bst2BI	CACGAG	2 cut(s)	355, 2153
Bst2UI	CCWGG	3 cut(s)	1190, 1376, 1517
Bst4CI	ACNGT	6 cut(s)	101, 784, 793, 1058, 1204, 1812
Bst6I	CTCTTC	4 cut(s)	48, 831, 1420, 2051
BstACI	GRCGYC	2 cut(s)	515, 707
BstAPI	GCANNNNNTGC	1 cut(s)	301
BstBAI	YACGTR	3 cut(s)	684, 1940, 2095
BstC8I	GCNNGC	5 cut(s)	206, 528, 849, 1218, 1860
BstDEI	CTNAG	7 cut(s)	498, 576, 978, 1257, 1887, 1947, 2106
BstDSI	CCRYGG	3 cut(s)	305, 624, 1000
BstENI	CCTNNNNNAGG	1 cut(s)	274
BstF5I	GGATG	6 cut(s)	655, 763, 780, 1777, 2134, 2514
BstFNI	CGCG	5 cut(s)	119, 204, 307, 523, 1371
BstH2I	RGCGCY	2 cut(s)	97, 136
BstHHI	GCGC	7 cut(s)	96, 121, 135, 153, 523, 829, 1373
BstMAI	GTCTC	3 cut(s)	975, 1096, 2495
BstMCI	CGRYCG	1 cut(s)	2126
BstMWI	GCNNNNNNNGC	9 cut(s)	91, 214, 301, 527, 1781, 1790, 1859, 1889, 1943
BstNI	CCWGG	3 cut(s)	1190, 1376, 1517
BstNSI	RCATGY	2 cut(s)	1685, 1921
BstSCI	CCNGG	3 cut(s)	1188, 1374, 1515
BstSFI	CTRYAG	1 cut(s)	1395
BstSLI	GKGCMC	1 cut(s)	1523
BstSNI	TACGTA	2 cut(s)	684, 2095
BstUI	CGCG	5 cut(s)	119, 204, 307, 523, 1371
BstV1I	GCAGC	5 cut(s)	1420, 1955, 2221, 2360, 2428
BstV2I	GAAGAC	5 cut(s)	939, 1078, 1205, 2030, 2373
BstX2I	RGATCY	3 cut(s)	405, 1959, 2116
BstYI	RGATCY	3 cut(s)	405, 1959, 2116
BsuRI	GGCC	2 cut(s)	530, 1313
BtgI	CCRYGG	3 cut(s)	305, 624, 1000
BtsCI	GGATG	6 cut(s)	655, 763, 780, 1777, 2134, 2514
BtsI	GCAGTG	3 cut(s)	934, 1227, 1548
BtsIMutI	CAGTG	5 cut(s)	934, 1209, 1227, 1548, 1808
Cac8I	GCNNGC	5 cut(s)	206, 528, 849, 1218, 1860
CaiI	CAGNNNCTG	1 cut(s)	334
CciI	TCATGA	1 cut(s)	2362
CfoI	GCGC	7 cut(s)	96, 121, 135, 153, 523, 829, 1373
Cfr13I	GGNCC	5 cut(s)	163, 1054, 1207, 1488, 2434
Cfr42I	CCGCGG	1 cut(s)	308
CseI	GACGC	4 cut(s)	125, 197, 504, 1377
Csp6I	GTAC	5 cut(s)	190, 250, 442, 1659, 1675
CviAII	CATG	8 cut(s)	464, 1001, 1016, 1274, 1682, 1904, 1918, 2363
CviQI	GTAC	5 cut(s)	190, 250, 442, 1659, 1675
DdeI	CTNAG	7 cut(s)	498, 576, 978, 1257, 1887, 1947, 2106
DraI	TTTAAA	1 cut(s)	1642
DriI	GACNNNNNGTC	1 cut(s)	2031
Eam1104I	CTCTTC	4 cut(s)	48, 831, 1420, 2051
Eam1105I	GACNNNNNGTC	1 cut(s)	2031
EarI	CTCTTC	4 cut(s)	48, 831, 1420, 2051
EciI	GGCGGA	1 cut(s)	2513
Ecl136II	GAGCTC	2 cut(s)	217, 2050
Eco105I	TACGTA	2 cut(s)	684, 2095
Eco130I	CCWWGG	2 cut(s)	1000, 2523
Eco24I	GRGCYC	2 cut(s)	219, 2052
Eco32I	GATATC	1 cut(s)	318
Eco47I	GGWCC	5 cut(s)	163, 1054, 1207, 1488, 2434
Eco53kI	GAGCTC	2 cut(s)	217, 2050
Eco57I	CTGAAG	1 cut(s)	2504
Eco72I	CACGTG	1 cut(s)	1940
Eco88I	CYCGRG	3 cut(s)	1536, 2051, 2082
EcoICRI	GAGCTC	2 cut(s)	217, 2050
EcoNI	CCTNNNNNAGG	1 cut(s)	274
EcoO109I	RGGNCCY	2 cut(s)	163, 2434
EcoRII	CCWGG	3 cut(s)	1188, 1374, 1515
EcoRV	GATATC	1 cut(s)	318
EcoT14I	CCWWGG	2 cut(s)	1000, 2523
EcoT22I	ATGCAT	3 cut(s)	297, 1603, 1639
EcoT38I	GRGCYC	2 cut(s)	219, 2052
ErhI	CCWWGG	2 cut(s)	1000, 2523
FaeI	CATG	8 cut(s)	467, 1004, 1019, 1277, 1685, 1907, 1921, 2366
FalI	AAGNNNNNCTT	2 cut(s)	42, 74
FaqI	GGGAC	2 cut(s)	2201, 2420
FatI	CATG	8 cut(s)	463, 1000, 1015, 1273, 1681, 1903, 1917, 2362
FauI	CCCGC	3 cut(s)	1686, 1851, 2446
FauNDI	CATATG	1 cut(s)	1694
FbaI	TGATCA	1 cut(s)	547
FblI	GTMKAC	3 cut(s)	432, 963, 1532
FokI	GGATG	6 cut(s)	662, 770, 787, 1784, 2141, 2521
FriOI	GRGCYC	2 cut(s)	219, 2052
FspBI	CTAG	4 cut(s)	212, 753, 1526, 1850
FspI	TGCGCA	1 cut(s)	828
GlaI	GCGC	7 cut(s)	95, 120, 134, 152, 522, 828, 1372
GsuI	CTGGAG	3 cut(s)	401, 1853, 2232
HaeII	RGCGCY	2 cut(s)	97, 136
HaeIII	GGCC	2 cut(s)	530, 1313
HapII	CCGG	4 cut(s)	171, 367, 436, 716
HgaI	GACGC	4 cut(s)	125, 197, 504, 1377
HhaI	GCGC	7 cut(s)	96, 121, 135, 153, 523, 829, 1373
Hin1I	GRCGYC	2 cut(s)	515, 707
Hin1II	CATG	8 cut(s)	467, 1004, 1019, 1277, 1685, 1907, 1921, 2366
Hin6I	GCGC	7 cut(s)	94, 119, 133, 151, 521, 827, 1371
HinP1I	GCGC	7 cut(s)	94, 119, 133, 151, 521, 827, 1371
HindIII	AAGCTT	1 cut(s)	182
HpaII	CCGG	4 cut(s)	171, 367, 436, 716
HphI	GGTGA	2 cut(s)	799, 2524
Hpy166II	GTNNAC	8 cut(s)	433, 681, 705, 964, 1207, 1501, 1533, 2092
Hpy8I	GTNNAC	8 cut(s)	433, 681, 705, 964, 1207, 1501, 1533, 2092
Hpy99I	CGWCG	3 cut(s)	119, 520, 712
HpyAV	CCTTC	6 cut(s)	133, 280, 394, 690, 742, 1861
HpyCH4III	ACNGT	6 cut(s)	101, 784, 793, 1058, 1204, 1812
HpyCH4IV	ACGT	6 cut(s)	683, 707, 1238, 1750, 1939, 2094
HpyF10VI	GCNNNNNNNGC	9 cut(s)	91, 214, 301, 527, 1781, 1790, 1859, 1889, 1943
HpyF3I	CTNAG	7 cut(s)	498, 576, 978, 1257, 1887, 1947, 2106
HpySE526I	ACGT	6 cut(s)	683, 707, 1238, 1750, 1939, 2094
Hsp92I	GRCGYC	2 cut(s)	515, 707
Hsp92II	CATG	8 cut(s)	467, 1004, 1019, 1277, 1685, 1907, 1921, 2366
HspAI	GCGC	7 cut(s)	94, 119, 133, 151, 521, 827, 1371
Kpn2I	TCCGGA	1 cut(s)	170
Ksp22I	TGATCA	1 cut(s)	547
KspI	CCGCGG	1 cut(s)	308
LguI	GCTCTTC	2 cut(s)	48, 2051
LmnI	GCTCC	5 cut(s)	340, 382, 1470, 1792, 2260
Lsp1109I	GCAGC	5 cut(s)	1420, 1955, 2221, 2360, 2428
LweI	GCATC	8 cut(s)	291, 640, 765, 1624, 1762, 1786, 2111, 2119
MaeI	CTAG	4 cut(s)	212, 753, 1526, 1850
MaeII	ACGT	6 cut(s)	683, 707, 1238, 1750, 1939, 2094
MaeIII	GTNAC	3 cut(s)	286, 352, 787
MflI	RGATCY	3 cut(s)	405, 1959, 2116
MhlI	GDGCHC	6 cut(s)	219, 394, 1523, 2052, 2083, 2532
MluCI	AATT	8 cut(s)	852, 1299, 1346, 1360, 1820, 2070, 2135, 2198
MlyI	GAGTC	6 cut(s)	355, 454, 503, 1538, 1998, 2223
MmeI	TCCRAC	3 cut(s)	138, 573, 931
Mph1103I	ATGCAT	3 cut(s)	297, 1603, 1639
MroI	TCCGGA	1 cut(s)	170
MroXI	GAANNNNTTC	1 cut(s)	57
MspA1I	CMGCKG	3 cut(s)	307, 1946, 2441
MspI	CCGG	4 cut(s)	171, 367, 436, 716
MspR9I	CCNGG	3 cut(s)	1190, 1376, 1517
Mva1269I	GAATGC	1 cut(s)	625
MvaI	CCWGG	3 cut(s)	1190, 1376, 1517
MvnI	CGCG	5 cut(s)	119, 204, 307, 523, 1371
MwoI	GCNNNNNNNGC	9 cut(s)	91, 214, 301, 527, 1781, 1790, 1859, 1889, 1943
NcoI	CCATGG	1 cut(s)	1000
NdeI	CATATG	1 cut(s)	1694
NlaIII	CATG	8 cut(s)	467, 1004, 1019, 1277, 1685, 1907, 1921, 2366
NlaIV	GGNNCC	9 cut(s)	407, 955, 1133, 1209, 1466, 1788, 1961, 2256, 2436
NmuCI	GTSAC	3 cut(s)	286, 352, 787
NsbI	TGCGCA	1 cut(s)	828
NsiI	ATGCAT	3 cut(s)	297, 1603, 1639
NspI	RCATGY	2 cut(s)	1685, 1921
PaeR7I	CTCGAG	2 cut(s)	2051, 2082
PagI	TCATGA	1 cut(s)	2362
PciSI	GCTCTTC	2 cut(s)	48, 2051
PcsI	WCGNNNNNNNCGW	3 cut(s)	111, 632, 1054
PctI	GAATGC	1 cut(s)	625
PdmI	GAANNNNTTC	1 cut(s)	57
PfeI	GAWTC	5 cut(s)	1159, 1444, 1997, 2324, 2479
Ple19I	CGATCG	1 cut(s)	2126
PleI	GAGTC	6 cut(s)	355, 454, 502, 1537, 1998, 2222
PmaCI	CACGTG	1 cut(s)	1940
PmlI	CACGTG	1 cut(s)	1940
PpsI	GAGTC	6 cut(s)	355, 454, 502, 1537, 1998, 2222
Ppu21I	YACGTR	3 cut(s)	684, 1940, 2095
PpuMI	RGGWCCY	2 cut(s)	163, 2434
PshBI	ATTAAT	1 cut(s)	2334
Psp124BI	GAGCTC	2 cut(s)	219, 2052
Psp5II	RGGWCCY	2 cut(s)	163, 2434
Psp6I	CCWGG	3 cut(s)	1188, 1374, 1515
PspCI	CACGTG	1 cut(s)	1940
PspGI	CCWGG	3 cut(s)	1188, 1374, 1515
PspN4I	GGNNCC	9 cut(s)	407, 955, 1133, 1209, 1466, 1788, 1961, 2256, 2436
PspPI	GGNCC	5 cut(s)	163, 1054, 1207, 1488, 2434
PspPPI	RGGWCCY	2 cut(s)	163, 2434
PspXI	VCTCGAGB	1 cut(s)	2082
PstNI	CAGNNNCTG	1 cut(s)	334
PsuI	RGATCY	3 cut(s)	405, 1959, 2116
PvuI	CGATCG	1 cut(s)	2126
PvuII	CAGCTG	1 cut(s)	1946
RsaI	GTAC	5 cut(s)	191, 251, 443, 1660, 1676
RsaNI	GTAC	5 cut(s)	190, 250, 442, 1659, 1675
SacI	GAGCTC	2 cut(s)	219, 2052
SacII	CCGCGG	1 cut(s)	308
SapI	GCTCTTC	2 cut(s)	48, 2051
Sau96I	GGNCC	5 cut(s)	163, 1054, 1207, 1488, 2434
SchI	GAGTC	6 cut(s)	355, 454, 503, 1538, 1998, 2223
ScrFI	CCNGG	3 cut(s)	1190, 1376, 1517
SduI	GDGCHC	6 cut(s)	219, 394, 1523, 2052, 2083, 2532
SfaNI	GCATC	8 cut(s)	291, 640, 765, 1624, 1762, 1786, 2111, 2119
SfcI	CTRYAG	1 cut(s)	1395
Sfr274I	CTCGAG	2 cut(s)	2051, 2082
Sfr303I	CCGCGG	1 cut(s)	308
SgrBI	CCGCGG	1 cut(s)	308
SinI	GGWCC	5 cut(s)	163, 1054, 1207, 1488, 2434
SlaI	CTCGAG	2 cut(s)	2051, 2082
SmlI	CTYRAG	9 cut(s)	12, 80, 158, 983, 1477, 2051, 2082, 2240, 2533
SmoI	CTYRAG	9 cut(s)	12, 80, 158, 983, 1477, 2051, 2082, 2240, 2533
SnaBI	TACGTA	2 cut(s)	684, 2095
Sse9I	AATT	8 cut(s)	852, 1299, 1346, 1360, 1820, 2070, 2135, 2198
SspI	AATATT	1 cut(s)	1668
SspMI	CTAG	4 cut(s)	212, 753, 1526, 1850
SstI	GAGCTC	2 cut(s)	219, 2052
StyD4I	CCNGG	3 cut(s)	1188, 1374, 1515
StyI	CCWWGG	2 cut(s)	1000, 2523
TaaI	ACNGT	6 cut(s)	101, 784, 793, 1058, 1204, 1812
TaiI	ACGT	6 cut(s)	686, 710, 1241, 1753, 1942, 2097
TaqI	TCGA	6 cut(s)	273, 375, 560, 1048, 2052, 2083
TasI	AATT	8 cut(s)	852, 1299, 1346, 1360, 1820, 2070, 2135, 2198
TauI	GCSGC	6 cut(s)	138, 307, 526, 1354, 1787, 2522
TfiI	GAWTC	5 cut(s)	1159, 1444, 1997, 2324, 2479
TscAI	CASTG	5 cut(s)	934, 1209, 1227, 1548, 1815
TseFI	GTSAC	3 cut(s)	286, 352, 787
TseI	GCWGC	5 cut(s)	1433, 1943, 2234, 2348, 2441
Tsp45I	GTSAC	3 cut(s)	286, 352, 787
TspGWI	ACGGA	1 cut(s)	353
TspRI	CASTG	5 cut(s)	934, 1209, 1227, 1548, 1815
VpaK11BI	GGWCC	5 cut(s)	163, 1054, 1207, 1488, 2434
VspI	ATTAAT	1 cut(s)	2334
XagI	CCTNNNNNAGG	1 cut(s)	274
XapI	RAATTY	2 cut(s)	1360, 2135
XbaI	TCTAGA	1 cut(s)	211
XceI	RCATGY	2 cut(s)	1685, 1921
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2227
XhoI	CTCGAG	2 cut(s)	2051, 2082
XmiI	GTMKAC	3 cut(s)	432, 963, 1532
XmnI	GAANNNNTTC	1 cut(s)	57
XspI	CTAG	4 cut(s)	212, 753, 1526, 1850
ZraI	GACGTC	1 cut(s)	708
Zsp2I	ATGCAT	3 cut(s)	297, 1603, 1639

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.