MD06G1144200.v1.1

Adenylate kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0001981
Physical Location & Seq
Forward (+)
28754759 .. 28757794
3036 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1144200.v1.1.491

Sequence Viewer

Length: 750 bp
ATGATCGTACCACGTCGTTTAGCCTCCACCGCCAGCTTTACCGCCGCGCCACCGCTGTACCGGCTTCTGAAACTCGCTCTCAGCCGATTCTTCACCGCCGCCGCGGCTCAGCCGCACTTCGATCACGACTACTACTATGGCCACCGAGACGACCGCGGGCGGCGCCTCGATCAACTTCCGGTGGCCGGCACGGAGGGCTCGGTTCCTGTACGAGGAGTGCAGTGGGCCTTCATAGGCAGTCCAATCGCCAAGAAACACGTCTACGCCGAGAGGCTCTCGAAGCTTCTAGAAGTTCCTCACATTTCCATGGCCGGTCTCGTCCGCCAAGACCTCAACCCTCGTTCTTCGCTTTACAAGCAGATTGCGAATGCTGTGAATCGAGGGGAGCTTGTTCCGGAAGAAATCATATTCGGGTTGTTATCGAAGAGATTGGAAGATGGGTATTGCAGAGGAGAAAGTGGATTCATACTGGATGGAATTCCTCGTTCTCGGATTCAAGCCGAAATTCTTTCCGAACTCGTTGAGATTGATCTAGTTGTGAATTTCAAATGCACAGAGCATTGCTTGGCGGAACACGAAAGAGAGAGTGCCTGGAGGGAGAAACTTCGAATCTTTGCTGAGCAGAGTAAGCCATTGGAAGATTACTACAAGAAACGGGATAAGCTTCTCGACTTTCAGGTGAGTAGTGCACCCGGGGAGACTTGGAAGGGGCTGTTGGCTACGTTACATCTACACTCCTCCCTGCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00730 ko01100 ko01110 ko01130 map00230 map00730 map01100 map01110 map01130
Pfam Domains
Protein Families
ADK

Protein Analysis

250

Amino Acids

28.22

Weight (kDa)

8.69

Isoelectric Point (pI)

46.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADK PF00406 78 - 220 2.4e-23 Adenylate kinase
AAA_17 PF13207 87 - 187 6.9e-07 AAA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 162
AccI GTMKAC 1 cut(s) 261
AccII CGCG 3 cut(s) 47, 104, 156
AccIII TCCGGA 1 cut(s) 394
AcoI YGGCCR 3 cut(s) 139, 183, 309
AcsI RAATTY 3 cut(s) 477, 504, 541
AcyI GRCGYC 1 cut(s) 163
AfaI GTAC 3 cut(s) 9, 59, 210
AfiI CCNNNNNNNGG 2 cut(s) 185, 212
AflIII ACRYGT 1 cut(s) 256
AgsI TTSAA 2 cut(s) 497, 547
AjiI CACGTC 2 cut(s) 14, 259
AjnI CCWGG 1 cut(s) 590
AjuI GAANNNNNNNTTGG 2 cut(s) 698, 730
AluBI AGCT 4 cut(s) 36, 283, 388, 664
AluI AGCT 4 cut(s) 36, 283, 388, 664
Alw21I GWGCWC 1 cut(s) 691
Alw26I GTCTC 3 cut(s) 141, 320, 692
Alw44I GTGCAC 1 cut(s) 687
Ama87I CYCGRG 1 cut(s) 692
Aor13HI TCCGGA 1 cut(s) 394
AoxI GGCC 4 cut(s) 139, 183, 225, 309
ApaLI GTGCAC 1 cut(s) 687
ApoI RAATTY 3 cut(s) 477, 504, 541
AspLEI GCGC 2 cut(s) 49, 165
AspS9I GGNCC 1 cut(s) 225
AsuC2I CCSGG 2 cut(s) 693, 694
AsuHPI GGTGA 2 cut(s) 85, 691
AsuII TTCGAA 1 cut(s) 607
AvaI CYCGRG 1 cut(s) 692
BaeGI GKGCMC 1 cut(s) 691
BaeI ACNNNNGTAYC 2 cut(s) 41, 74
BalI TGGCCA 1 cut(s) 141
BanI GGYRCC 1 cut(s) 162
BanII GRGCYC 1 cut(s) 200
Bbv12I GWGCWC 1 cut(s) 691
BccI CCATC 2 cut(s) 431, 467
BcgI CGANNNNNNTGC 2 cut(s) 226, 260
BciT130I CCWGG 1 cut(s) 592
BcnI CCSGG 2 cut(s) 693, 694
BcoDI GTCTC 3 cut(s) 141, 320, 692
BfaI CTAG 2 cut(s) 287, 533
BfmI CTRYAG 1 cut(s) 746
BfoI RGCGCY 1 cut(s) 166
BglI GCCNNNNNGGC 1 cut(s) 104
BisI GCNGC 6 cut(s) 45, 99, 102, 105, 113, 161
BlpI GCTNAGC 2 cut(s) 108, 618
BlsI GCNGC 6 cut(s) 46, 100, 103, 106, 114, 162
Bme1390I CCNGG 3 cut(s) 592, 693, 694
BmeT110I CYCGRG 1 cut(s) 692
BmgBI CACGTC 2 cut(s) 14, 259
BmgT120I GGNCC 1 cut(s) 225
BmiI GGNNCC 2 cut(s) 164, 204
BmrFI CCNGG 3 cut(s) 592, 693, 694
BplI GAGNNNNNCTC 2 cut(s) 260, 292
BpmI CTGGAG 1 cut(s) 613
Bpu1102I GCTNAGC 2 cut(s) 108, 618
Bpu14I TTCGAA 1 cut(s) 607
BpuMI CCSGG 2 cut(s) 693, 694
BsaHI GRCGYC 1 cut(s) 163
BsaI GGTCTC 1 cut(s) 320
BsaJI CCNNGG 5 cut(s) 102, 154, 306, 692, 693
BsaWI WCCGGW 2 cut(s) 178, 394
Bsc4I CCNNNNNNNGG 2 cut(s) 185, 212
Bse118I RCCGGY 3 cut(s) 60, 185, 311
Bse1I ACTGG 1 cut(s) 474
Bse3DI GCAATG 1 cut(s) 559
BseAI TCCGGA 1 cut(s) 394
BseBI CCWGG 1 cut(s) 592
BseDI CCNNGG 5 cut(s) 102, 154, 306, 692, 693
BseGI GGATG 1 cut(s) 478
BseLI CCNNNNNNNGG 2 cut(s) 185, 212
BseMI GCAATG 1 cut(s) 559
BseMII CTCAG 3 cut(s) 94, 122, 609
BseNI ACTGG 1 cut(s) 474
BseRI GAGGAG 3 cut(s) 228, 465, 727
BseSI GKGCMC 1 cut(s) 691
BsgI GTGCAG 1 cut(s) 239
Bsh1236I CGCG 3 cut(s) 47, 104, 156
Bsh1285I CGRYCG 1 cut(s) 154
BshFI GGCC 4 cut(s) 141, 185, 227, 311
BshNI GGYRCC 1 cut(s) 162
BsiEI CGRYCG 1 cut(s) 154
BsiHKAI GWGCWC 1 cut(s) 691
BsiHKCI CYCGRG 1 cut(s) 692
BsiSI CCGG 6 cut(s) 61, 179, 186, 312, 395, 693
BslI CCNNNNNNNGG 2 cut(s) 185, 212
BsmAI GTCTC 3 cut(s) 141, 320, 692
BsmBI CGTCTC 1 cut(s) 141
BsmI GAATGC 1 cut(s) 373
BsnI GGCC 4 cut(s) 141, 185, 227, 311
Bso31I GGTCTC 1 cut(s) 320
BsoBI CYCGRG 1 cut(s) 692
Bsp119I TTCGAA 1 cut(s) 607
Bsp1286I GDGCHC 2 cut(s) 200, 691
Bsp13I TCCGGA 1 cut(s) 394
Bsp143I GATC 4 cut(s) 3, 121, 169, 529
Bsp1720I GCTNAGC 2 cut(s) 108, 618
Bsp19I CCATGG 1 cut(s) 306
BspANI GGCC 4 cut(s) 141, 185, 227, 311
BspCNI CTCAG 3 cut(s) 93, 121, 610
BspEI TCCGGA 1 cut(s) 394
BspFNI CGCG 3 cut(s) 47, 104, 156
BspLI GGNNCC 2 cut(s) 164, 204
BspT104I TTCGAA 1 cut(s) 607
BspT107I GGYRCC 1 cut(s) 162
BspTNI GGTCTC 1 cut(s) 320
BsrDI GCAATG 1 cut(s) 559
BsrFI RCCGGY 3 cut(s) 60, 185, 311
BsrI ACTGG 1 cut(s) 474
BssAI RCCGGY 3 cut(s) 60, 185, 311
BssECI CCNNGG 5 cut(s) 102, 154, 306, 692, 693
BssMI GATC 4 cut(s) 3, 121, 169, 529
BssNI GRCGYC 1 cut(s) 163
BssT1I CCWWGG 1 cut(s) 306
Bst2UI CCWGG 1 cut(s) 592
Bst6I CTCTTC 1 cut(s) 419
BstACI GRCGYC 1 cut(s) 163
BstBI TTCGAA 1 cut(s) 607
BstC8I GCNNGC 3 cut(s) 34, 158, 187
BstDEI CTNAG 3 cut(s) 80, 108, 618
BstDSI CCRYGG 3 cut(s) 102, 154, 306
BstENI CCTNNNNNAGG 1 cut(s) 210
BstF5I GGATG 1 cut(s) 478
BstFNI CGCG 3 cut(s) 47, 104, 156
BstH2I RGCGCY 1 cut(s) 166
BstHHI GCGC 2 cut(s) 49, 165
BstKTI GATC 4 cut(s) 6, 124, 172, 532
BstMAI GTCTC 3 cut(s) 141, 320, 692
BstMBI GATC 4 cut(s) 3, 121, 169, 529
BstMCI CGRYCG 1 cut(s) 154
BstMWI GCNNNNNNNGC 8 cut(s) 29, 61, 104, 162, 195, 280, 355, 628
BstNI CCWGG 1 cut(s) 592
BstSCI CCNGG 3 cut(s) 590, 691, 692
BstSFI CTRYAG 1 cut(s) 746
BstSLI GKGCMC 1 cut(s) 691
BstUI CGCG 3 cut(s) 47, 104, 156
BsuRI GGCC 4 cut(s) 141, 185, 227, 311
BtgI CCRYGG 3 cut(s) 102, 154, 306
BtrI CACGTC 2 cut(s) 14, 259
BtsCI GGATG 1 cut(s) 478
BtsI GCAGTG 1 cut(s) 227
BtsIMutI CAGTG 1 cut(s) 227
Cac8I GCNNGC 3 cut(s) 34, 158, 187
CfoI GCGC 2 cut(s) 49, 165
Cfr10I RCCGGY 3 cut(s) 60, 185, 311
Cfr13I GGNCC 1 cut(s) 225
Cfr42I CCGCGG 2 cut(s) 105, 157
Cfr9I CCCGGG 1 cut(s) 692
Csp6I GTAC 3 cut(s) 8, 58, 209
CviAII CATG 1 cut(s) 307
CviQI GTAC 3 cut(s) 8, 58, 209
DdeI CTNAG 3 cut(s) 80, 108, 618
DinI GGCGCC 1 cut(s) 164
DpnI GATC 4 cut(s) 5, 123, 171, 531
DpnII GATC 4 cut(s) 3, 121, 169, 529
EaeI YGGCCR 3 cut(s) 139, 183, 309
Eam1104I CTCTTC 1 cut(s) 419
EarI CTCTTC 1 cut(s) 419
EciI GGCGGA 2 cut(s) 311, 584
Eco130I CCWWGG 1 cut(s) 306
Eco24I GRGCYC 1 cut(s) 200
Eco31I GGTCTC 1 cut(s) 320
Eco88I CYCGRG 1 cut(s) 692
EcoNI CCTNNNNNAGG 1 cut(s) 210
EcoRI GAATTC 1 cut(s) 477
EcoRII CCWGG 1 cut(s) 590
EcoT14I CCWWGG 1 cut(s) 306
EcoT38I GRGCYC 1 cut(s) 200
EgeI GGCGCC 1 cut(s) 164
EheI GGCGCC 1 cut(s) 164
ErhI CCWWGG 1 cut(s) 306
Esp3I CGTCTC 1 cut(s) 141
FaeI CATG 1 cut(s) 310
FaiI YATR 5 cut(s) 138, 233, 308, 407, 467
FatI CATG 1 cut(s) 306
FauI CCCGC 1 cut(s) 149
FblI GTMKAC 1 cut(s) 261
Fnu4HI GCNGC 6 cut(s) 45, 99, 102, 105, 113, 161
FokI GGATG 1 cut(s) 485
FriOI GRGCYC 1 cut(s) 200
Fsp4HI GCNGC 6 cut(s) 45, 99, 102, 105, 113, 161
FspBI CTAG 2 cut(s) 287, 533
GlaI GCGC 2 cut(s) 48, 164
GluI GCNGC 6 cut(s) 45, 99, 102, 105, 113, 161
GsuI CTGGAG 1 cut(s) 613
HaeII RGCGCY 1 cut(s) 166
HaeIII GGCC 4 cut(s) 141, 185, 227, 311
HapII CCGG 6 cut(s) 61, 179, 186, 312, 395, 693
HhaI GCGC 2 cut(s) 49, 165
Hin1I GRCGYC 1 cut(s) 163
Hin1II CATG 1 cut(s) 310
Hin6I GCGC 2 cut(s) 47, 163
HinP1I GCGC 2 cut(s) 47, 163
HindIII AAGCTT 2 cut(s) 281, 662
HinfI GANTC 5 cut(s) 87, 376, 462, 493, 609
HpaII CCGG 6 cut(s) 61, 179, 186, 312, 395, 693
HphI GGTGA 2 cut(s) 85, 691
Hpy166II GTNNAC 2 cut(s) 262, 689
Hpy188I TCNGA 3 cut(s) 69, 492, 514
Hpy188III TCNNGA 5 cut(s) 125, 277, 287, 395, 668
Hpy8I GTNNAC 2 cut(s) 262, 689
Hpy99I CGWCG 1 cut(s) 18
HpyAV CCTTC 2 cut(s) 238, 700
HpyCH4IV ACGT 3 cut(s) 13, 258, 722
HpyCH4V TGCA 4 cut(s) 220, 447, 552, 689
HpyF10VI GCNNNNNNNGC 8 cut(s) 29, 61, 104, 162, 195, 280, 355, 628
HpyF3I CTNAG 3 cut(s) 80, 108, 618
HpySE526I ACGT 3 cut(s) 13, 258, 722
Hsp92I GRCGYC 1 cut(s) 163
Hsp92II CATG 1 cut(s) 310
HspAI GCGC 2 cut(s) 47, 163
KasI GGCGCC 1 cut(s) 162
Kpn2I TCCGGA 1 cut(s) 394
KroI GCCGGC 1 cut(s) 185
KroNI GCCGGC 1 cut(s) 187
KspI CCGCGG 2 cut(s) 105, 157
Kzo9I GATC 4 cut(s) 3, 121, 169, 529
LmnI GCTCC 1 cut(s) 385
MaeI CTAG 2 cut(s) 287, 533
MaeII ACGT 3 cut(s) 13, 258, 722
MaeIII GTNAC 1 cut(s) 723
MalI GATC 4 cut(s) 5, 123, 171, 531
MboI GATC 4 cut(s) 3, 121, 169, 529
MboII GAAGA 6 cut(s) 82, 336, 410, 436, 446, 650
MhlI GDGCHC 2 cut(s) 200, 691
MlsI TGGCCA 1 cut(s) 141
MluCI AATT 3 cut(s) 477, 504, 541
MluNI TGGCCA 1 cut(s) 141
Mly113I GGCGCC 1 cut(s) 163
Mox20I TGGCCA 1 cut(s) 141
MroI TCCGGA 1 cut(s) 394
MroNI GCCGGC 1 cut(s) 185
MscI TGGCCA 1 cut(s) 141
MslI CAYNNNNRTG 1 cut(s) 305
Msp20I TGGCCA 1 cut(s) 141
MspA1I CMGCKG 3 cut(s) 55, 104, 156
MspI CCGG 6 cut(s) 61, 179, 186, 312, 395, 693
MspR9I CCNGG 3 cut(s) 592, 693, 694
Mva1269I GAATGC 1 cut(s) 373
MvaI CCWGG 1 cut(s) 592
MvnI CGCG 3 cut(s) 47, 104, 156
MwoI GCNNNNNNNGC 8 cut(s) 29, 61, 104, 162, 195, 280, 355, 628
NaeI GCCGGC 1 cut(s) 187
NarI GGCGCC 1 cut(s) 163
NciI CCSGG 2 cut(s) 693, 694
NcoI CCATGG 1 cut(s) 306
NdeII GATC 4 cut(s) 3, 121, 169, 529
NgoMIV GCCGGC 1 cut(s) 185
NlaIII CATG 1 cut(s) 310
NlaIV GGNNCC 2 cut(s) 164, 204
NmeAIII GCCGAG 1 cut(s) 292
NspV TTCGAA 1 cut(s) 607
PcsI WCGNNNNNNNCGW 1 cut(s) 264
PctI GAATGC 1 cut(s) 373
PdiI GCCGGC 1 cut(s) 187
PfeI GAWTC 5 cut(s) 87, 376, 462, 493, 609
PkrI GCNGC 6 cut(s) 46, 100, 103, 106, 114, 162
PluTI GGCGCC 1 cut(s) 166
Psp6I CCWGG 1 cut(s) 590
PspGI CCWGG 1 cut(s) 590
PspN4I GGNNCC 2 cut(s) 164, 204
PspPI GGNCC 1 cut(s) 225
RsaI GTAC 3 cut(s) 9, 59, 210
RsaNI GTAC 3 cut(s) 8, 58, 209
RseI CAYNNNNRTG 1 cut(s) 305
SacII CCGCGG 2 cut(s) 105, 157
SatI GCNGC 6 cut(s) 45, 99, 102, 105, 113, 161
Sau3AI GATC 4 cut(s) 3, 121, 169, 529
Sau96I GGNCC 1 cut(s) 225
ScrFI CCNGG 3 cut(s) 592, 693, 694
SduI GDGCHC 2 cut(s) 200, 691
SetI ASST 9 cut(s) 16, 38, 261, 285, 333, 390, 666, 681, 725
SfcI CTRYAG 1 cut(s) 746
SfoI GGCGCC 1 cut(s) 164
Sfr303I CCGCGG 2 cut(s) 105, 157
SfuI TTCGAA 1 cut(s) 607
SgrBI CCGCGG 2 cut(s) 105, 157
SmaI CCCGGG 1 cut(s) 694
SmiMI CAYNNNNRTG 1 cut(s) 305
Sse9I AATT 3 cut(s) 477, 504, 541
SspDI GGCGCC 1 cut(s) 162
SspMI CTAG 2 cut(s) 287, 533
StyD4I CCNGG 3 cut(s) 590, 691, 692
StyI CCWWGG 1 cut(s) 306
TaiI ACGT 3 cut(s) 16, 261, 725
TaqI TCGA 7 cut(s) 120, 168, 278, 379, 422, 607, 669
TasI AATT 3 cut(s) 477, 504, 541
TauI GCSGC 6 cut(s) 47, 101, 104, 107, 115, 163
TfiI GAWTC 5 cut(s) 87, 376, 462, 493, 609
TscAI CASTG 1 cut(s) 227
TspDTI ATGAA 2 cut(s) 220, 454
TspGWI ACGGA 1 cut(s) 206
TspMI CCCGGG 1 cut(s) 692
TspRI CASTG 1 cut(s) 227
VneI GTGCAC 1 cut(s) 687
XagI CCTNNNNNAGG 1 cut(s) 210
XapI RAATTY 3 cut(s) 477, 504, 541
XbaI TCTAGA 1 cut(s) 286
XmaI CCCGGG 1 cut(s) 692
XmiI GTMKAC 1 cut(s) 261
XspI CTAG 2 cut(s) 287, 533
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.