MD17G1014400.v1.1

Belongs to the adenylate kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0001981
Physical Location & Seq
Forward (+)
1194948 .. 1196685
1738 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1014400.v1.1.491

Sequence Viewer

Length: 846 bp
ATGGCCGTTCTCAGCCGCCTCCAAGTAGTTGCATCCCAGATCACGCGCCGGAGCCTCGGGAATATGGAGCGCCGAGCCTACGGATCCGCAGTGGCTCAGCTGCAGTACGACGACGACTATTACTACGACGATCAGGATGAAGAGGAGGAACGGAGGAAGGCGACTCGAAATCGGATGCTGGACTCGGACGGGCGGGTCCCGGGGAGAGGTGTGCAGTGGGTGCTGATCGGAGACAGAGGCGCGAAGAAGCACTTGTACGCCGAGAGGCTCTCGAAGCTTCTGGAAGTTCCGCACATTTCTATGGGATCCCTCGTCCGCCAGGAGCTCAATCCTCGCTCTTCTCTCTACAAGCAGATTGCGAATGCAGTCAATGAAGGGAAGCTTGTTCCGGACGAAGTGATATTTGCATTGTTGTCGAAGAGGTTGGAAGAAGGATACTATCGCGGCGAAAACGGATTCATTCTCGATGGGATTCCTCGAACAAGAACTCAAGCTGAGATTGTTGACCAAATTGCCGAGATTGATTTAGTGGTGAATTTCAAGTGCTCAAATGAGCACTTGGTGAAGAACAATCTAGGAACCCGAAACTTCTCTGCTTGCCGAGAATATCTTAGCATGGGCAACTCCGTTCGAAATCTGAATCTGCAGCCAAAGGAAGAACTTCTGAAATCTTCACCTGCTGATGCAGAGCAGACCAAGTCGCTGGAAGATTACTACAGAAAACAGAACAAACTCATCGATTTTCAGATCAAAGCTGCGCCCGGAGAAACCTGGCAAGGGCTGTTGGCTGCGTTACATCTTCAGCATATAAACGCTGTCAGTTCTTCCCAGAAGCTCACCGCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00730 ko01100 ko01110 ko01130 map00230 map00730 map01100 map01110 map01130
Pfam Domains
Protein Families
ADK

Protein Analysis

282

Amino Acids

31.9

Weight (kDa)

7.74

Isoelectric Point (pI)

46.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADK PF00406 75 - 243 2.5e-24 Adenylate kinase
AAA_17 PF13207 86 - 194 2.3e-14 AAA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 685
AasI GACNNNNNNGTC 1 cut(s) 194
Acc36I ACCTGC 1 cut(s) 685
AccII CGCG 4 cut(s) 46, 242, 444, 842
AccIII TCCGGA 1 cut(s) 388
AciI CCGC 7 cut(s) 16, 87, 193, 290, 316, 444, 840
AclWI GGATC 4 cut(s) 78, 91, 300, 313
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 535
AcuI CTGAAG 1 cut(s) 785
AdeI CACNNNGTG 2 cut(s) 562, 843
AfaI GTAC 2 cut(s) 107, 257
AfiI CCNNNNNNNGG 2 cut(s) 206, 777
AgsI TTSAA 1 cut(s) 541
AjnI CCWGG 2 cut(s) 318, 770
AluBI AGCT 7 cut(s) 100, 277, 325, 382, 494, 755, 835
AluI AGCT 7 cut(s) 100, 277, 325, 382, 494, 755, 835
Alw21I GWGCWC 3 cut(s) 327, 548, 558
Alw26I GTCTC 1 cut(s) 225
AlwI GGATC 4 cut(s) 78, 91, 300, 313
Ama87I CYCGRG 2 cut(s) 56, 199
Aor13HI TCCGGA 1 cut(s) 388
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 4 cut(s) 100, 646, 755, 788
ApoI RAATTY 1 cut(s) 535
Asp700I GAANNNNTTC 1 cut(s) 660
AspLEI GCGC 4 cut(s) 48, 72, 242, 760
AspS9I GGNCC 1 cut(s) 196
AsuC2I CCSGG 3 cut(s) 200, 201, 762
AsuHPI GGTGA 4 cut(s) 544, 574, 666, 829
AsuII TTCGAA 1 cut(s) 631
AvaI CYCGRG 2 cut(s) 56, 199
AvaII GGWCC 1 cut(s) 196
BamHI GGATCC 2 cut(s) 83, 305
BanII GRGCYC 1 cut(s) 327
BarI GAAGNNNNNNTAC 2 cut(s) 239, 271
Bbv12I GWGCWC 3 cut(s) 327, 548, 558
BbvI GCAGC 4 cut(s) 87, 658, 742, 775
BccI CCATC 1 cut(s) 461
BcgI CGANNNNNNTGC 2 cut(s) 396, 430
BciT130I CCWGG 2 cut(s) 320, 772
BciVI GTATCC 1 cut(s) 428
BcnI CCSGG 3 cut(s) 200, 201, 762
BcoDI GTCTC 1 cut(s) 225
BfaI CTAG 1 cut(s) 575
BfmI CTRYAG 3 cut(s) 101, 644, 715
BfoI RGCGCY 1 cut(s) 73
BfuAI ACCTGC 1 cut(s) 685
BfuI GTATCC 1 cut(s) 428
BisI GCNGC 6 cut(s) 16, 101, 445, 647, 756, 789
BlpI GCTNAGC 1 cut(s) 96
BlsI GCNGC 6 cut(s) 17, 102, 446, 648, 757, 790
Bme1390I CCNGG 5 cut(s) 200, 201, 320, 762, 772
Bme18I GGWCC 1 cut(s) 196
BmeT110I CYCGRG 2 cut(s) 56, 199
BmgT120I GGNCC 1 cut(s) 196
BmiI GGNNCC 6 cut(s) 53, 85, 197, 198, 307, 580
BmrFI CCNGG 5 cut(s) 200, 201, 320, 762, 772
BmsI GCATC 3 cut(s) 41, 165, 673
BplI GAGNNNNNCTC 2 cut(s) 254, 286
Bpu1102I GCTNAGC 1 cut(s) 96
Bpu14I TTCGAA 1 cut(s) 631
BpuEI CTTGAG 1 cut(s) 474
BpuMI CCSGG 3 cut(s) 200, 201, 762
Bsa29I ATCGAT 1 cut(s) 738
BsaJI CCNNGG 3 cut(s) 55, 199, 200
BsaWI WCCGGW 1 cut(s) 388
Bsc4I CCNNNNNNNGG 2 cut(s) 206, 777
BseAI TCCGGA 1 cut(s) 388
BseBI CCWGG 2 cut(s) 320, 772
BseCI ATCGAT 1 cut(s) 738
BseDI CCNNGG 3 cut(s) 55, 199, 200
BseGI GGATG 3 cut(s) 32, 142, 180
BseLI CCNNNNNNNGG 2 cut(s) 206, 777
BseMII CTCAG 3 cut(s) 25, 110, 486
BseRI GAGGAG 1 cut(s) 158
BseXI GCAGC 4 cut(s) 87, 658, 742, 775
BsgI GTGCAG 1 cut(s) 233
Bsh1236I CGCG 4 cut(s) 46, 242, 444, 842
BshFI GGCC 1 cut(s) 5
BshVI ATCGAT 1 cut(s) 738
BsiHKAI GWGCWC 3 cut(s) 327, 548, 558
BsiHKCI CYCGRG 2 cut(s) 56, 199
BsiSI CCGG 4 cut(s) 49, 200, 389, 762
BslFI GGGAC 1 cut(s) 182
BslI CCNNNNNNNGG 2 cut(s) 206, 777
BsmAI GTCTC 1 cut(s) 225
BsmFI GGGAC 1 cut(s) 182
BsmI GAATGC 1 cut(s) 367
BsnI GGCC 1 cut(s) 5
BsoBI CYCGRG 2 cut(s) 56, 199
Bsp119I TTCGAA 1 cut(s) 631
Bsp1286I GDGCHC 3 cut(s) 327, 548, 558
Bsp13I TCCGGA 1 cut(s) 388
Bsp143I GATC 6 cut(s) 39, 83, 130, 225, 305, 747
Bsp1720I GCTNAGC 1 cut(s) 96
BspACI CCGC 7 cut(s) 16, 87, 193, 290, 316, 444, 840
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 3 cut(s) 24, 109, 487
BspDI ATCGAT 1 cut(s) 738
BspEI TCCGGA 1 cut(s) 388
BspFNI CGCG 4 cut(s) 46, 242, 444, 842
BspLI GGNNCC 6 cut(s) 53, 85, 197, 198, 307, 580
BspMAI CTGCAG 2 cut(s) 105, 648
BspMI ACCTGC 1 cut(s) 685
BspPI GGATC 4 cut(s) 78, 91, 300, 313
BspQI GCTCTTC 1 cut(s) 343
BspT104I TTCGAA 1 cut(s) 631
BssECI CCNNGG 3 cut(s) 55, 199, 200
BssMI GATC 6 cut(s) 39, 83, 130, 225, 305, 747
Bst2UI CCWGG 2 cut(s) 320, 772
Bst6I CTCTTC 3 cut(s) 135, 343, 413
BstAPI GCANNNNNTGC 1 cut(s) 220
BstBI TTCGAA 1 cut(s) 631
BstC8I GCNNGC 1 cut(s) 598
BstDEI CTNAG 4 cut(s) 11, 96, 495, 611
BstF5I GGATG 3 cut(s) 32, 142, 180
BstFNI CGCG 4 cut(s) 46, 242, 444, 842
BstH2I RGCGCY 1 cut(s) 73
BstHHI GCGC 4 cut(s) 48, 72, 242, 760
BstKTI GATC 6 cut(s) 42, 86, 133, 228, 308, 750
BstMAI GTCTC 1 cut(s) 225
BstMBI GATC 6 cut(s) 39, 83, 130, 225, 305, 747
BstMWI GCNNNNNNNGC 2 cut(s) 220, 274
BstNI CCWGG 2 cut(s) 320, 772
BstSCI CCNGG 5 cut(s) 198, 199, 318, 760, 770
BstSFI CTRYAG 3 cut(s) 101, 644, 715
BstUI CGCG 4 cut(s) 46, 242, 444, 842
BstV1I GCAGC 4 cut(s) 87, 658, 742, 775
BstX2I RGATCY 2 cut(s) 83, 305
BstXI CCANNNNNNTGG 1 cut(s) 703
BstYI RGATCY 2 cut(s) 83, 305
Bsu15I ATCGAT 1 cut(s) 738
BsuI GTATCC 1 cut(s) 428
BsuRI GGCC 1 cut(s) 5
BsuTUI ATCGAT 1 cut(s) 738
BtsCI GGATG 3 cut(s) 32, 142, 180
BtsI GCAGTG 2 cut(s) 96, 221
BtsIMutI CAGTG 2 cut(s) 96, 221
BveI ACCTGC 1 cut(s) 685
Cac8I GCNNGC 1 cut(s) 598
CfoI GCGC 4 cut(s) 48, 72, 242, 760
Cfr13I GGNCC 1 cut(s) 196
Cfr9I CCCGGG 1 cut(s) 199
ClaI ATCGAT 1 cut(s) 738
Csp6I GTAC 2 cut(s) 106, 256
CviAII CATG 1 cut(s) 616
CviQI GTAC 2 cut(s) 106, 256
DdeI CTNAG 4 cut(s) 11, 96, 495, 611
DpnI GATC 6 cut(s) 41, 85, 132, 227, 307, 749
DpnII GATC 6 cut(s) 39, 83, 130, 225, 305, 747
DraIII CACNNNGTG 2 cut(s) 562, 843
DrdI GACNNNNNNGTC 1 cut(s) 194
DseDI GACNNNNNNGTC 1 cut(s) 194
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 3 cut(s) 135, 343, 413
EarI CTCTTC 3 cut(s) 135, 343, 413
EciI GGCGGA 1 cut(s) 305
Ecl136II GAGCTC 1 cut(s) 325
Eco24I GRGCYC 1 cut(s) 327
Eco47I GGWCC 1 cut(s) 196
Eco53kI GAGCTC 1 cut(s) 325
Eco57I CTGAAG 1 cut(s) 785
Eco88I CYCGRG 2 cut(s) 56, 199
EcoICRI GAGCTC 1 cut(s) 325
EcoO109I RGGNCCY 1 cut(s) 196
EcoRII CCWGG 2 cut(s) 318, 770
EcoT38I GRGCYC 1 cut(s) 327
FaeI CATG 1 cut(s) 619
FaiI YATR 5 cut(s) 65, 302, 617, 807, 809
FalI AAGNNNNNCTT 4 cut(s) 236, 268, 366, 398
FaqI GGGAC 1 cut(s) 182
FatI CATG 1 cut(s) 615
FauI CCCGC 1 cut(s) 186
Fnu4HI GCNGC 6 cut(s) 16, 101, 445, 647, 756, 789
FokI GGATG 3 cut(s) 19, 149, 187
FriOI GRGCYC 1 cut(s) 327
Fsp4HI GCNGC 6 cut(s) 16, 101, 445, 647, 756, 789
FspBI CTAG 1 cut(s) 575
GlaI GCGC 4 cut(s) 47, 71, 241, 759
GluI GCNGC 6 cut(s) 16, 101, 445, 647, 756, 789
HaeII RGCGCY 1 cut(s) 73
HaeIII GGCC 1 cut(s) 5
HapII CCGG 4 cut(s) 49, 200, 389, 762
HhaI GCGC 4 cut(s) 48, 72, 242, 760
Hin1II CATG 1 cut(s) 619
Hin6I GCGC 4 cut(s) 46, 70, 240, 758
HinP1I GCGC 4 cut(s) 46, 70, 240, 758
HincII GTYRAC 1 cut(s) 505
HindII GTYRAC 1 cut(s) 505
HindIII AAGCTT 2 cut(s) 275, 380
HinfI GANTC 5 cut(s) 163, 182, 456, 472, 640
HpaII CCGG 4 cut(s) 49, 200, 389, 762
HphI GGTGA 4 cut(s) 544, 574, 666, 829
Hpy166II GTNNAC 1 cut(s) 505
Hpy188I TCNGA 6 cut(s) 174, 187, 230, 639, 666, 747
Hpy188III TCNNGA 6 cut(s) 58, 134, 271, 281, 389, 464
Hpy8I GTNNAC 1 cut(s) 505
Hpy99I CGWCG 3 cut(s) 113, 116, 131
HpyAV CCTTC 3 cut(s) 151, 368, 425
HpyCH4V TGCA 7 cut(s) 32, 103, 214, 365, 407, 646, 686
HpyF10VI GCNNNNNNNGC 2 cut(s) 220, 274
HpyF3I CTNAG 4 cut(s) 11, 96, 495, 611
Hsp92II CATG 1 cut(s) 619
HspAI GCGC 4 cut(s) 46, 70, 240, 758
KflI GGGWCCC 1 cut(s) 196
Kpn2I TCCGGA 1 cut(s) 388
Kzo9I GATC 6 cut(s) 39, 83, 130, 225, 305, 747
LguI GCTCTTC 1 cut(s) 343
LmnI GCTCC 3 cut(s) 51, 67, 322
Lsp1109I GCAGC 4 cut(s) 87, 658, 742, 775
LweI GCATC 3 cut(s) 41, 165, 673
MaeI CTAG 1 cut(s) 575
MaeIII GTNAC 1 cut(s) 792
MalI GATC 6 cut(s) 41, 85, 132, 227, 307, 749
MboI GATC 6 cut(s) 39, 83, 130, 225, 305, 747
MflI RGATCY 2 cut(s) 83, 305
MhlI GDGCHC 3 cut(s) 327, 548, 558
MluCI AATT 2 cut(s) 510, 535
MlyI GAGTC 2 cut(s) 157, 176
MmeI TCCRAC 1 cut(s) 405
MroI TCCGGA 1 cut(s) 388
MroXI GAANNNNTTC 1 cut(s) 660
MslI CAYNNNNRTG 1 cut(s) 299
MspA1I CMGCKG 1 cut(s) 100
MspI CCGG 4 cut(s) 49, 200, 389, 762
MspR9I CCNGG 5 cut(s) 200, 201, 320, 762, 772
Mva1269I GAATGC 1 cut(s) 367
MvaI CCWGG 2 cut(s) 320, 772
MvnI CGCG 4 cut(s) 46, 242, 444, 842
MwoI GCNNNNNNNGC 2 cut(s) 220, 274
NciI CCSGG 3 cut(s) 200, 201, 762
NdeII GATC 6 cut(s) 39, 83, 130, 225, 305, 747
NlaIII CATG 1 cut(s) 619
NlaIV GGNNCC 6 cut(s) 53, 85, 197, 198, 307, 580
NmeAIII GCCGAG 4 cut(s) 98, 286, 541, 626
NspV TTCGAA 1 cut(s) 631
PaqCI CACCTGC 1 cut(s) 685
PciSI GCTCTTC 1 cut(s) 343
PctI GAATGC 1 cut(s) 367
PdmI GAANNNNTTC 1 cut(s) 660
PfeI GAWTC 3 cut(s) 456, 472, 640
PflFI GACNNNGTC 1 cut(s) 697
PkrI GCNGC 6 cut(s) 17, 102, 446, 648, 757, 790
PleI GAGTC 2 cut(s) 157, 176
PpsI GAGTC 2 cut(s) 157, 176
PpuMI RGGWCCY 1 cut(s) 196
Psp124BI GAGCTC 1 cut(s) 327
Psp5II RGGWCCY 1 cut(s) 196
Psp6I CCWGG 2 cut(s) 318, 770
PspGI CCWGG 2 cut(s) 318, 770
PspN4I GGNNCC 6 cut(s) 53, 85, 197, 198, 307, 580
PspPI GGNCC 1 cut(s) 196
PspPPI RGGWCCY 1 cut(s) 196
PstI CTGCAG 2 cut(s) 105, 648
PsuI RGATCY 2 cut(s) 83, 305
PsyI GACNNNGTC 1 cut(s) 697
PvuII CAGCTG 1 cut(s) 100
RsaI GTAC 2 cut(s) 107, 257
RsaNI GTAC 2 cut(s) 106, 256
RseI CAYNNNNRTG 1 cut(s) 299
SacI GAGCTC 1 cut(s) 327
SapI GCTCTTC 1 cut(s) 343
SatI GCNGC 6 cut(s) 16, 101, 445, 647, 756, 789
Sau3AI GATC 6 cut(s) 39, 83, 130, 225, 305, 747
Sau96I GGNCC 1 cut(s) 196
SchI GAGTC 2 cut(s) 157, 176
ScrFI CCNGG 5 cut(s) 200, 201, 320, 762, 772
SduI GDGCHC 3 cut(s) 327, 548, 558
SfaNI GCATC 3 cut(s) 41, 165, 673
SfcI CTRYAG 3 cut(s) 101, 644, 715
SfuI TTCGAA 1 cut(s) 631
SinI GGWCC 1 cut(s) 196
SmaI CCCGGG 1 cut(s) 201
SmiMI CAYNNNNRTG 1 cut(s) 299
SmlI CTYRAG 1 cut(s) 489
SmoI CTYRAG 1 cut(s) 489
Sse9I AATT 2 cut(s) 510, 535
SsiI CCGC 7 cut(s) 16, 87, 193, 290, 316, 444, 840
SspMI CTAG 1 cut(s) 575
SstI GAGCTC 1 cut(s) 327
StyD4I CCNGG 5 cut(s) 198, 199, 318, 760, 770
TaqI TCGA 7 cut(s) 166, 272, 416, 465, 478, 631, 738
TasI AATT 2 cut(s) 510, 535
TauI GCSGC 2 cut(s) 18, 447
TfiI GAWTC 3 cut(s) 456, 472, 640
TscAI CASTG 2 cut(s) 96, 221
TseI GCWGC 4 cut(s) 100, 646, 755, 788
TspDTI ATGAA 3 cut(s) 153, 387, 448
TspGWI ACGGA 4 cut(s) 96, 166, 468, 616
TspMI CCCGGG 1 cut(s) 199
TspRI CASTG 2 cut(s) 96, 221
Tth111I GACNNNGTC 1 cut(s) 697
VpaK11BI GGWCC 1 cut(s) 196
XapI RAATTY 1 cut(s) 535
XmaI CCCGGG 1 cut(s) 199
XmnI GAANNNNTTC 1 cut(s) 660
XspI CTAG 1 cut(s) 575
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.