MD12G1127500.v1.1

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0004550
Physical Location & Seq
Reverse (-)
20257149 .. 20261457
4309 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1127500.v1.1.491

Sequence Viewer

Length: 2586 bp
ATGGGAAGGGAAATCGCCAAAAAATGTGCAGGTGTACCATTGGTGGCAAAGGTGTTGGGAAATATGATGTGTCGTAAACTGAGTGATGGATGGCAGTCAATTCAAGACAGTGCAATATGGGATTTGCCAGAAGGAGAAGAAAGAATATTTTTAGTTTTGAAGTTGAGTTTTGATGAATTGAGATCACCATCTTTGAAACAATGTTTTGCATATTGTTCAATGTTCGTCCAAGATGATATTATTTATAAGGAGGAATTAATCCAACTTTGGATGGCTCAAGGATTGCTCCAACCTTCTCACAACGAAAGTATGGAGGATGTAGGGAATGAAAATTTTAATATTCTACTGGAGAACTCCTTTTTTCAAGATGTTGAAATGAAGGGAAATACTGTTTACTATTGCAAGATGCACGATCTTATGCACAATCTTGCAGAACATGTGTCAAAATCACAGAGCAAGAACTTCCCTGAGATTCGACAGGAGGCAAAGCTCTCTACATCGGCACTGCATGTAATTCCAAACGGAGGTTTGAATGGTGAAATTCTCGGTGACATCTTATCAACATTTGAAGGTTTGCGCGTGGGTAAGGAGAGAGGTCGTGGAATAGAGGAGTTGGCTGGCTTAAAGCAATTGAAAGGCCGATTATCCATATTTAATCTAGGGCTTGTGAGAGATGGAGAGGAAGCAAAGAAAGCAAAACTAGCAGAGAAGACAAATCTGCGTGAATTAGTCTTTGCATGGGGATATGAGCAAAGGTCAAGAATTAATGATGAGGATGTACTAGAAGGCCTTCGACCAGCACACCCTAAACTGGAATCGCTAGAGATTCAATGCTTTATGGGTAAGAAATTTGCATCCTGGATGATGGGGAGTCTCATTCCTTCATTAAAAAGATTACATATCGATTCACCTAAGAATCTAATTGAATGGGTGGAAGCAGCAGAAAACATTGTGCTCTTTCCATGCCTCGAGGAGCTGTCTCTGACCAACTGCGATCAATTGAGAAGTGCTCCCAGTCATTTTCCATCTCTGAAAAAGTTAAAGATAGTTTCCATGGGTAATGGCATGCCAATAGCAAATATAAGCAGCAACCTGACCACTCTTACTTCTCTCACAATAAGAAGTATAAGGGGACTTGATTGTCTGCCAGAAGGGATGTTAAAAAACAACAAGAATCTTGCATATTTGTGTATAGCAGATTGTCCGGATTTAACTTGTATTACTACGGATGATGTATTTGGTTGTTGTGCTTCTCTTCGGTCAGTGCATATTTCCGGGTGTGATAATCTTAGGTGCCTGCCTGATGGGCTACAAACTCTTGTCTCTCTTAACGAGTTGAGTATACAGATTTGCAATAGTCTAGAGCTCATTCCAGACATGCACGGTCTCACTTCTCTTCGGTCAGTGAGTATTTCCGCGTGTCATAATCTTAGGTGCCTGCCTGATGGGCTACAAACTCTTGTCTCTCTTAACAAGTTGAGTATAAAGAGTTGCAATAGTCTAGAGCGCATTCCAGACATGCACGGTCTCACTTCTCTTCGTGAGTTGACCATAGAATATTGCTCCAAGCTAACGTCCATTCAATTTGAACAGGGCCTCCCATCTCTTTGTGATTTAATTATTGACAATTGTCGTGAATTATCAAGCCTGCCGAGTGGGTTAGAATATTGTACCTCTCTTCAGAATTTGAGAATATCTGGTTATGAACAGGGCCTCCCACCATCCCTCCACTCTCTGGAGATATCTGGTTGCAAGAGTCTAGAGTCCATTCCATTTGAACAGGGCCTCCCATCTCTCTATATTCGCAGATGTCCTCAACTATCAAGCCTGCCGAGTGGGTTAGAATATTGTACTTCTCTTCAGGATTTGACAATATCTGATTGTGAAATGCTATCATCCATTGGGATTCACGGCCTCCCACCATCCCTCCACTCTCTGGGCATAAATGGTTGCAACAGTCTAGAATCTATTCCTAGTTTAGAAAACCTCACACACCTCCGTAAGTTGGAGATAGTTGATTGTCATGGGTTAAAAAACCTGCCCGGTGGATTAAAAACCCTCACCAGCTTGAAGGAATTGGAAATTGGTGGGTTTTGGAAGGAGCTCGATTCATTCCCTCCTTTTGAGGTTATTCCACAACTTGAATCATTAATGTTGCGTGGTTGGCCTACGCTTCAGTCTCTGCCTGAAAATGTTCAACACTTGACTTCTCTAACTCATTTGGAAATAAGGCACTTCGACGGAATGGAGTCTCTTCCGGAGTGGTTGGGGAACCTTGCATCTCTTCAGTCCCTACGGATATGGAGATGCAAGAATCTGAAGTATCTACCTACAGCTGAAGCTATGAAATGCCTCACCAAATTAAAAAAAGTATACATTGCTGATTGTCCCGTTCTAAAAGAGAGATGCAACAAGAAGAGCGGCCCGGAATGGCACAAGATTTCTCATATTCCAACTATTGGTATGTTTTTCCTTTTCCGGTTCTTAAATCAATTACGCACAATGTATTCTATTTTCTTCTCTTACTTTTTCATTAAAGTGAATCACTTGTTATGTGGTATGTGTGTTTCTGTTTCATTTTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03460 ko04014 map03460 map04014
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

862

Amino Acids

96.44

Weight (kDa)

6.07

Isoelectric Point (pI)

52.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_DRP PF23559 74 - 144 2.8e-23 Disease resistance protein Winged helix domain
LRR_R13L1-DRL21 PF25019 202 - 331 1.6e-25 R13L1/DRL21 LRRs
LRR_14 PF23598 387 - 591 1.7e-08 Leucine-rich repeat region
LRR_RPS2 PF23247 464 - 551 1.6e-06 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_14 PF23598 597 - 789 6.1e-11 Leucine-rich repeat region
LRR_RPS2 PF23247 665 - 796 8.5e-06 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_14 PF23598 714 - 806 6.5e-08 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 730 - 789 1e-07 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 246
AarI CACCTGC 1 cut(s) 20
AasI GACNNNNNNGTC 1 cut(s) 1140
AbsI CCTCGAGG 1 cut(s) 968
Acc36I ACCTGC 2 cut(s) 20, 2046
AccB1I GGYRCC 2 cut(s) 1293, 1434
AccB7I CCANNNNNTGG 3 cut(s) 1735, 1936, 2459
AccBSI CCGCTC 1 cut(s) 2421
AccI GTMKAC 2 cut(s) 1342, 2373
AccII CGCG 2 cut(s) 579, 1418
AccIII TCCGGA 2 cut(s) 1204, 2257
AciI CCGC 2 cut(s) 1416, 2421
AcsI RAATTY 4 cut(s) 331, 540, 848, 1684
AcuI CTGAAG 6 cut(s) 1664, 1844, 2159, 2270, 2339, 2358
AfaI GTAC 4 cut(s) 36, 780, 1672, 1852
AfiI CCNNNNNNNGG 6 cut(s) 524, 811, 1735, 1936, 2005, 2459
AflIII ACRYGT 1 cut(s) 436
AjnI CCWGG 1 cut(s) 857
AjuI GAANNNNNNNTTGG 2 cut(s) 2067, 2099
AloI GAACNNNNNNTCC 6 cut(s) 1581, 1613, 1698, 1730, 1770, 1802
AluBI AGCT 8 cut(s) 490, 976, 1366, 1570, 2067, 2104, 2336, 2342
AluI AGCT 8 cut(s) 490, 976, 1366, 1570, 2067, 2104, 2336, 2342
Alw21I GWGCWC 4 cut(s) 957, 1012, 1368, 2106
Alw26I GTCTC 8 cut(s) 878, 984, 1327, 1391, 1468, 1532, 2184, 2256
AlwNI CAGNNNCTG 1 cut(s) 2182
Ama87I CYCGRG 1 cut(s) 968
Aor13HI TCCGGA 2 cut(s) 1204, 2257
AoxI GGCC 8 cut(s) 637, 787, 1594, 1711, 1783, 1912, 2165, 2422
ApeKI GCWGC 2 cut(s) 938, 1086
ApoI RAATTY 4 cut(s) 331, 540, 848, 1684
AseI ATTAAT 3 cut(s) 257, 765, 2150
Asp700I GAANNNNTTC 3 cut(s) 789, 1968, 2193
AspLEI GCGC 2 cut(s) 579, 1509
AspS9I GGNCC 4 cut(s) 1594, 1711, 1783, 2423
AsuC2I CCSGG 3 cut(s) 1276, 2043, 2426
AsuHPI GGTGA 6 cut(s) 177, 548, 560, 900, 2053, 2347
AvaI CYCGRG 1 cut(s) 968
BanI GGYRCC 2 cut(s) 1293, 1434
BanII GRGCYC 2 cut(s) 1368, 2106
BbsI GAAGAC 1 cut(s) 716
Bbv12I GWGCWC 4 cut(s) 957, 1012, 1368, 2106
BbvI GCAGC 2 cut(s) 950, 1098
BceAI ACGGC 1 cut(s) 1927
BciT130I CCWGG 1 cut(s) 859
BcnI CCSGG 3 cut(s) 1276, 2043, 2426
BcoDI GTCTC 8 cut(s) 878, 984, 1327, 1391, 1468, 1532, 2184, 2256
BfaI CTAG 9 cut(s) 659, 701, 782, 821, 1361, 1502, 1760, 1961, 1974
BfmI CTRYAG 1 cut(s) 2331
BfuAI ACCTGC 2 cut(s) 20, 2046
BglI GCCNNNNNGGC 2 cut(s) 1306, 1447
BisI GCNGC 3 cut(s) 939, 1087, 2422
BlsI GCNGC 3 cut(s) 940, 1088, 2423
Bme1390I CCNGG 4 cut(s) 859, 1276, 2043, 2426
BmeT110I CYCGRG 1 cut(s) 968
BmgT120I GGNCC 4 cut(s) 1594, 1711, 1783, 2423
BmiI GGNNCC 3 cut(s) 1295, 1436, 2273
BmrFI CCNGG 4 cut(s) 859, 1276, 2043, 2426
BmrI ACTGGG 1 cut(s) 1008
BmsI GCATC 5 cut(s) 396, 863, 2288, 2297, 2396
BmuI ACTGGG 1 cut(s) 1008
BoxI GACNNNNGTC 1 cut(s) 1629
BpiI GAAGAC 1 cut(s) 716
BplI GAGNNNNNCTC 2 cut(s) 994, 1026
BpmI CTGGAG 2 cut(s) 368, 1757
BpuEI CTTGAG 1 cut(s) 261
BpuMI CCSGG 3 cut(s) 1276, 2043, 2426
Bsa29I ATCGAT 1 cut(s) 903
BsaBI GATNNNNATC 1 cut(s) 187
BsaI GGTCTC 2 cut(s) 1391, 1532
BsaJI CCNNGG 1 cut(s) 1053
BsaWI WCCGGW 3 cut(s) 1204, 2257, 2478
Bsc4I CCNNNNNNNGG 6 cut(s) 524, 811, 1735, 1936, 2005, 2459
Bse1I ACTGG 3 cut(s) 351, 816, 1014
Bse3DI GCAATG 1 cut(s) 2376
Bse8I GATNNNNATC 1 cut(s) 187
BseAI TCCGGA 2 cut(s) 1204, 2257
BseBI CCWGG 1 cut(s) 859
BseCI ATCGAT 1 cut(s) 903
BseDI CCNNGG 1 cut(s) 1053
BseJI GATNNNNATC 1 cut(s) 187
BseLI CCNNNNNNNGG 6 cut(s) 524, 811, 1735, 1936, 2005, 2459
BseMI GCAATG 1 cut(s) 2376
BseMII CTCAG 2 cut(s) 71, 459
BseNI ACTGG 3 cut(s) 351, 816, 1014
BseRI GAGGAG 2 cut(s) 623, 986
BseXI GCAGC 2 cut(s) 950, 1098
BsgI GTGCAG 1 cut(s) 48
Bsh1236I CGCG 2 cut(s) 579, 1418
BshFI GGCC 8 cut(s) 639, 789, 1596, 1713, 1785, 1914, 2167, 2424
BshNI GGYRCC 2 cut(s) 1293, 1434
BshVI ATCGAT 1 cut(s) 903
BsiHKAI GWGCWC 4 cut(s) 957, 1012, 1368, 2106
BsiHKCI CYCGRG 1 cut(s) 968
BsiSI CCGG 6 cut(s) 1205, 1275, 2043, 2258, 2426, 2479
BslFI GGGAC 3 cut(s) 1146, 2275, 2373
BslI CCNNNNNNNGG 6 cut(s) 524, 811, 1735, 1936, 2005, 2459
BsmAI GTCTC 8 cut(s) 878, 984, 1327, 1391, 1468, 1532, 2184, 2256
BsmFI GGGAC 3 cut(s) 1146, 2275, 2373
BsmI GAATGC 1 cut(s) 1509
BsnI GGCC 8 cut(s) 639, 789, 1596, 1713, 1785, 1914, 2167, 2424
Bso31I GGTCTC 2 cut(s) 1391, 1532
BsoBI CYCGRG 1 cut(s) 968
Bsp1286I GDGCHC 4 cut(s) 957, 1012, 1368, 2106
Bsp13I TCCGGA 2 cut(s) 1204, 2257
Bsp143I GATC 3 cut(s) 182, 412, 994
Bsp19I CCATGG 1 cut(s) 1053
BspACI CCGC 2 cut(s) 1416, 2421
BspANI GGCC 8 cut(s) 639, 789, 1596, 1713, 1785, 1914, 2167, 2424
BspCNI CTCAG 2 cut(s) 72, 460
BspDI ATCGAT 1 cut(s) 903
BspEI TCCGGA 2 cut(s) 1204, 2257
BspFNI CGCG 2 cut(s) 579, 1418
BspLI GGNNCC 3 cut(s) 1295, 1436, 2273
BspMI ACCTGC 2 cut(s) 20, 2046
BspQI GCTCTTC 1 cut(s) 2411
BspT107I GGYRCC 2 cut(s) 1293, 1434
BspTNI GGTCTC 2 cut(s) 1391, 1532
BsrBI CCGCTC 1 cut(s) 2421
BsrDI GCAATG 1 cut(s) 2376
BsrI ACTGG 3 cut(s) 351, 816, 1014
BssECI CCNNGG 1 cut(s) 1053
BssMI GATC 3 cut(s) 182, 412, 994
BssNAI GTATAC 2 cut(s) 1343, 2374
BssT1I CCWWGG 1 cut(s) 1053
Bst1107I GTATAC 2 cut(s) 1343, 2374
Bst2UI CCWGG 1 cut(s) 859
Bst4CI ACNGT 5 cut(s) 110, 391, 1385, 1526, 1958
Bst6I CTCTTC 8 cut(s) 1260, 1401, 1542, 1683, 1863, 2259, 2289, 2411
BstC8I GCNNGC 6 cut(s) 619, 1067, 1298, 1439, 1649, 1829
BstDEI CTNAG 5 cut(s) 80, 468, 912, 1289, 1430
BstDSI CCRYGG 1 cut(s) 1053
BstFNI CGCG 2 cut(s) 579, 1418
BstHHI GCGC 2 cut(s) 579, 1509
BstKTI GATC 3 cut(s) 185, 415, 997
BstMAI GTCTC 8 cut(s) 878, 984, 1327, 1391, 1468, 1532, 2184, 2256
BstMBI GATC 3 cut(s) 182, 412, 994
BstMWI GCNNNNNNNGC 5 cut(s) 692, 701, 1306, 1447, 2164
BstNI CCWGG 1 cut(s) 859
BstNSI RCATGY 5 cut(s) 440, 512, 1069, 1381, 1522
BstPAI GACNNNNGTC 1 cut(s) 1629
BstSCI CCNGG 4 cut(s) 857, 1274, 2041, 2424
BstSFI CTRYAG 1 cut(s) 2331
BstUI CGCG 2 cut(s) 579, 1418
BstV1I GCAGC 2 cut(s) 950, 1098
BstV2I GAAGAC 1 cut(s) 716
BstZ17I GTATAC 2 cut(s) 1343, 2374
Bsu15I ATCGAT 1 cut(s) 903
BsuRI GGCC 8 cut(s) 639, 789, 1596, 1713, 1785, 1914, 2167, 2424
BsuTUI ATCGAT 1 cut(s) 903
BtgI CCRYGG 1 cut(s) 1053
BtsI GCAGTG 1 cut(s) 503
BtsIMutI CAGTG 4 cut(s) 115, 503, 1269, 1410
BveI ACCTGC 2 cut(s) 20, 2046
Cac8I GCNNGC 6 cut(s) 619, 1067, 1298, 1439, 1649, 1829
CaiI CAGNNNCTG 1 cut(s) 2182
CfoI GCGC 2 cut(s) 579, 1509
Cfr13I GGNCC 4 cut(s) 1594, 1711, 1783, 2423
ClaI ATCGAT 1 cut(s) 903
Csp6I GTAC 4 cut(s) 35, 779, 1671, 1851
CviAII CATG 9 cut(s) 437, 509, 738, 963, 1054, 1066, 1378, 1519, 2024
CviQI GTAC 4 cut(s) 35, 779, 1671, 1851
DdeI CTNAG 5 cut(s) 80, 468, 912, 1289, 1430
DpnI GATC 3 cut(s) 184, 414, 996
DpnII GATC 3 cut(s) 182, 412, 994
DrdI GACNNNNNNGTC 1 cut(s) 1140
DseDI GACNNNNNNGTC 1 cut(s) 1140
Eam1104I CTCTTC 8 cut(s) 1260, 1401, 1542, 1683, 1863, 2259, 2289, 2411
EarI CTCTTC 8 cut(s) 1260, 1401, 1542, 1683, 1863, 2259, 2289, 2411
Ecl136II GAGCTC 2 cut(s) 1366, 2104
Eco130I CCWWGG 1 cut(s) 1053
Eco147I AGGCCT 1 cut(s) 789
Eco24I GRGCYC 2 cut(s) 1368, 2106
Eco31I GGTCTC 2 cut(s) 1391, 1532
Eco32I GATATC 1 cut(s) 1743
Eco53kI GAGCTC 2 cut(s) 1366, 2104
Eco57I CTGAAG 6 cut(s) 1664, 1844, 2159, 2270, 2339, 2358
Eco88I CYCGRG 1 cut(s) 968
EcoICRI GAGCTC 2 cut(s) 1366, 2104
EcoO109I RGGNCCY 3 cut(s) 1594, 1711, 1783
EcoRII CCWGG 1 cut(s) 857
EcoRV GATATC 1 cut(s) 1743
EcoT14I CCWWGG 1 cut(s) 1053
EcoT38I GRGCYC 2 cut(s) 1368, 2106
ErhI CCWWGG 1 cut(s) 1053
FaeI CATG 9 cut(s) 440, 512, 741, 966, 1057, 1069, 1381, 1522, 2027
FaqI GGGAC 3 cut(s) 1146, 2275, 2373
FatI CATG 9 cut(s) 436, 508, 737, 962, 1053, 1065, 1377, 1518, 2023
FblI GTMKAC 2 cut(s) 1342, 2373
Fnu4HI GCNGC 3 cut(s) 939, 1087, 2422
FriOI GRGCYC 2 cut(s) 1368, 2106
Fsp4HI GCNGC 3 cut(s) 939, 1087, 2422
FspBI CTAG 9 cut(s) 659, 701, 782, 821, 1361, 1502, 1760, 1961, 1974
GlaI GCGC 2 cut(s) 578, 1508
GluI GCNGC 3 cut(s) 939, 1087, 2422
GsuI CTGGAG 2 cut(s) 368, 1757
HaeIII GGCC 8 cut(s) 639, 789, 1596, 1713, 1785, 1914, 2167, 2424
HapII CCGG 6 cut(s) 1205, 1275, 2043, 2258, 2426, 2479
HhaI GCGC 2 cut(s) 579, 1509
Hin1II CATG 9 cut(s) 440, 512, 741, 966, 1057, 1069, 1381, 1522, 2027
Hin6I GCGC 2 cut(s) 577, 1507
HinP1I GCGC 2 cut(s) 577, 1507
HincII GTYRAC 1 cut(s) 1548
HindII GTYRAC 1 cut(s) 1548
HpaII CCGG 6 cut(s) 1205, 1275, 2043, 2258, 2426, 2479
HphI GGTGA 6 cut(s) 177, 548, 560, 900, 2053, 2347
Hpy166II GTNNAC 6 cut(s) 35, 77, 394, 1343, 1548, 2374
Hpy188I TCNGA 5 cut(s) 984, 1032, 1683, 1879, 2319
Hpy8I GTNNAC 6 cut(s) 35, 77, 394, 1343, 1548, 2374
Hpy99I CGWCG 1 cut(s) 2243
HpyCH4III ACNGT 5 cut(s) 110, 391, 1385, 1526, 1958
HpyCH4IV ACGT 1 cut(s) 1574
HpyF10VI GCNNNNNNNGC 5 cut(s) 692, 701, 1306, 1447, 2164
HpyF3I CTNAG 5 cut(s) 80, 468, 912, 1289, 1430
HpySE526I ACGT 1 cut(s) 1574
Hsp92II CATG 9 cut(s) 440, 512, 741, 966, 1057, 1069, 1381, 1522, 2027
HspAI GCGC 2 cut(s) 577, 1507
Kpn2I TCCGGA 2 cut(s) 1204, 2257
Kzo9I GATC 3 cut(s) 182, 412, 994
LguI GCTCTTC 1 cut(s) 2411
LmnI GCTCC 5 cut(s) 291, 973, 1015, 1568, 2101
Lsp1109I GCAGC 2 cut(s) 950, 1098
LweI GCATC 5 cut(s) 396, 863, 2288, 2297, 2396
MaeI CTAG 9 cut(s) 659, 701, 782, 821, 1361, 1502, 1760, 1961, 1974
MaeII ACGT 1 cut(s) 1574
MaeIII GTNAC 1 cut(s) 548
MalI GATC 3 cut(s) 184, 414, 996
MbiI CCGCTC 1 cut(s) 2421
MboI GATC 3 cut(s) 182, 412, 994
MfeI CAATTG 3 cut(s) 629, 998, 1627
MhlI GDGCHC 4 cut(s) 957, 1012, 1368, 2106
MlyI GAGTC 4 cut(s) 880, 1765, 1772, 2258
MmeI TCCRAC 4 cut(s) 286, 313, 1986, 2477
MroI TCCGGA 2 cut(s) 1204, 2257
MroXI GAANNNNTTC 3 cut(s) 789, 1968, 2193
MslI CAYNNNNRTG 2 cut(s) 1186, 2537
MspA1I CMGCKG 1 cut(s) 2336
MspI CCGG 6 cut(s) 1205, 1275, 2043, 2258, 2426, 2479
MspR9I CCNGG 4 cut(s) 859, 1276, 2043, 2426
MunI CAATTG 3 cut(s) 629, 998, 1627
Mva1269I GAATGC 1 cut(s) 1509
MvaI CCWGG 1 cut(s) 859
MvnI CGCG 2 cut(s) 579, 1418
MwoI GCNNNNNNNGC 5 cut(s) 692, 701, 1306, 1447, 2164
NciI CCSGG 3 cut(s) 1276, 2043, 2426
NcoI CCATGG 1 cut(s) 1053
NdeII GATC 3 cut(s) 182, 412, 994
NlaIII CATG 9 cut(s) 440, 512, 741, 966, 1057, 1069, 1381, 1522, 2027
NlaIV GGNNCC 3 cut(s) 1295, 1436, 2273
NmeAIII GCCGAG 2 cut(s) 1677, 1857
NmuCI GTSAC 1 cut(s) 548
NspI RCATGY 5 cut(s) 440, 512, 1069, 1381, 1522
PaeI GCATGC 1 cut(s) 1069
PaeR7I CTCGAG 1 cut(s) 968
PaqCI CACCTGC 1 cut(s) 20
PceI AGGCCT 1 cut(s) 789
PciI ACATGT 1 cut(s) 436
PciSI GCTCTTC 1 cut(s) 2411
PctI GAATGC 1 cut(s) 1509
PdmI GAANNNNTTC 3 cut(s) 789, 1968, 2193
PflMI CCANNNNNTGG 3 cut(s) 1735, 1936, 2459
PfoI TCCNGGA 1 cut(s) 857
PkrI GCNGC 3 cut(s) 940, 1088, 2423
PleI GAGTC 4 cut(s) 879, 1764, 1771, 2257
PpsI GAGTC 4 cut(s) 879, 1764, 1771, 2257
PscI ACATGT 1 cut(s) 436
PshAI GACNNNNGTC 1 cut(s) 1629
PshBI ATTAAT 3 cut(s) 257, 765, 2150
PsiI TTATAA 1 cut(s) 246
Psp124BI GAGCTC 2 cut(s) 1368, 2106
Psp6I CCWGG 1 cut(s) 857
PspGI CCWGG 1 cut(s) 857
PspN4I GGNNCC 3 cut(s) 1295, 1436, 2273
PspPI GGNCC 4 cut(s) 1594, 1711, 1783, 2423
PspXI VCTCGAGB 1 cut(s) 968
PstNI CAGNNNCTG 1 cut(s) 2182
PvuII CAGCTG 1 cut(s) 2336
RsaI GTAC 4 cut(s) 36, 780, 1672, 1852
RsaNI GTAC 4 cut(s) 35, 779, 1671, 1851
RseI CAYNNNNRTG 2 cut(s) 1186, 2537
SacI GAGCTC 2 cut(s) 1368, 2106
SapI GCTCTTC 1 cut(s) 2411
SatI GCNGC 3 cut(s) 939, 1087, 2422
Sau3AI GATC 3 cut(s) 182, 412, 994
Sau96I GGNCC 4 cut(s) 1594, 1711, 1783, 2423
SchI GAGTC 4 cut(s) 880, 1765, 1772, 2258
ScrFI CCNGG 4 cut(s) 859, 1276, 2043, 2426
SduI GDGCHC 4 cut(s) 957, 1012, 1368, 2106
SfaNI GCATC 5 cut(s) 396, 863, 2288, 2297, 2396
SfcI CTRYAG 1 cut(s) 2331
Sfr274I CTCGAG 1 cut(s) 968
SlaI CTCGAG 1 cut(s) 968
SmiMI CAYNNNNRTG 2 cut(s) 1186, 2537
SmlI CTYRAG 2 cut(s) 276, 968
SmoI CTYRAG 2 cut(s) 276, 968
SphI GCATGC 1 cut(s) 1069
SseBI AGGCCT 1 cut(s) 789
SsiI CCGC 2 cut(s) 1416, 2421
SspI AATATT 5 cut(s) 147, 340, 1559, 1667, 1847
SspMI CTAG 9 cut(s) 659, 701, 782, 821, 1361, 1502, 1760, 1961, 1974
SstI GAGCTC 2 cut(s) 1368, 2106
StuI AGGCCT 1 cut(s) 789
StyD4I CCNGG 4 cut(s) 857, 1274, 2041, 2424
StyI CCWWGG 1 cut(s) 1053
TaaI ACNGT 5 cut(s) 110, 391, 1385, 1526, 1958
TaiI ACGT 1 cut(s) 1577
TaqI TCGA 6 cut(s) 475, 793, 903, 969, 2106, 2238
TaqII GACCGA 2 cut(s) 1248, 1389
TatI WGTACW 2 cut(s) 778, 1850
TauI GCSGC 1 cut(s) 2424
TscAI CASTG 4 cut(s) 115, 510, 1269, 1410
TseFI GTSAC 1 cut(s) 548
TseI GCWGC 2 cut(s) 938, 1086
Tsp45I GTSAC 1 cut(s) 548
TspDTI ATGAA 9 cut(s) 189, 342, 392, 873, 1719, 2100, 2360, 2521, 2565
TspGWI ACGGA 5 cut(s) 537, 1241, 1988, 2256, 2311
TspRI CASTG 4 cut(s) 115, 510, 1269, 1410
Van91I CCANNNNNTGG 3 cut(s) 1735, 1936, 2459
VspI ATTAAT 3 cut(s) 257, 765, 2150
XapI RAATTY 4 cut(s) 331, 540, 848, 1684
XbaI TCTAGA 4 cut(s) 1360, 1501, 1759, 1960
XceI RCATGY 5 cut(s) 440, 512, 1069, 1381, 1522
XhoI CTCGAG 1 cut(s) 968
XmiI GTMKAC 2 cut(s) 1342, 2373
XmnI GAANNNNTTC 3 cut(s) 789, 1968, 2193
XspI CTAG 9 cut(s) 659, 701, 782, 821, 1361, 1502, 1760, 1961, 1974
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.