Rmu_sc0005425.1_g000014

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005425.1
OG0004550
Physical Location & Seq
Forward (+)
68154 .. 68459
306 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0005425.1_g000014.1.cds

Sequence Viewer

Length: 306 bp
ctgttgaaagaccttgacgtggcccgcgcttgccaatctgccgcggaggctcgagctcgtgcggcccgcgtggtacggaataaccacgctagtcagatagagactcttcgcggcaccttgaatgatcgggccactcgcatgggggagatcaggctccagaagttagacttagaagcccagattcgacatctccaagctcatttggcccatgtgaacaacgagcttagcgcggaggaagcccaactggacggtcccttggctgcctccgaagcattgactcgccgcttcgcattaactaactattga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

101

Amino Acids

11.21

Weight (kDa)

8.21

Isoelectric Point (pI)

40.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 113
AccII CGCG 5 cut(s) 27, 44, 69, 111, 230
AciI CCGC 8 cut(s) 25, 42, 44, 62, 67, 111, 230, 283
AfaI GTAC 1 cut(s) 75
AfiI CCNNNNNNNGG 1 cut(s) 19
AgsI TTSAA 2 cut(s) 7, 121
AjiI CACGTC 1 cut(s) 19
AluBI AGCT 3 cut(s) 56, 197, 223
AluI AGCT 3 cut(s) 56, 197, 223
Alw21I GWGCWC 1 cut(s) 58
Alw26I GTCTC 1 cut(s) 95
Ama87I CYCGRG 1 cut(s) 51
AoxI GGCC 4 cut(s) 21, 63, 129, 204
ApeKI GCWGC 1 cut(s) 260
AspLEI GCGC 2 cut(s) 29, 230
AspS9I GGNCC 5 cut(s) 22, 64, 129, 205, 251
AvaI CYCGRG 1 cut(s) 51
AvaII GGWCC 1 cut(s) 251
BanI GGYRCC 1 cut(s) 113
BanII GRGCYC 1 cut(s) 58
BauI CACGAG 1 cut(s) 57
Bbv12I GWGCWC 1 cut(s) 58
BbvI GCAGC 1 cut(s) 247
BcoDI GTCTC 1 cut(s) 95
BfaI CTAG 1 cut(s) 90
BglI GCCNNNNNGGC 1 cut(s) 47
BisI GCNGC 5 cut(s) 42, 63, 112, 261, 283
BlpI GCTNAGC 1 cut(s) 224
BlsI GCNGC 5 cut(s) 43, 64, 113, 262, 284
Bme18I GGWCC 1 cut(s) 251
BmeT110I CYCGRG 1 cut(s) 51
BmgBI CACGTC 1 cut(s) 19
BmgT120I GGNCC 5 cut(s) 22, 64, 129, 205, 251
BmiI GGNNCC 3 cut(s) 115, 155, 253
BpmI CTGGAG 1 cut(s) 140
Bpu1102I GCTNAGC 1 cut(s) 224
BsaJI CCNNGG 2 cut(s) 42, 255
Bsc4I CCNNNNNNNGG 1 cut(s) 19
Bse1I ACTGG 1 cut(s) 249
BseDI CCNNGG 2 cut(s) 42, 255
BseLI CCNNNNNNNGG 1 cut(s) 19
BseNI ACTGG 1 cut(s) 249
BseXI GCAGC 1 cut(s) 247
Bsh1236I CGCG 5 cut(s) 27, 44, 69, 111, 230
BshFI GGCC 4 cut(s) 23, 65, 131, 206
BshNI GGYRCC 1 cut(s) 113
BsiHKAI GWGCWC 1 cut(s) 58
BsiHKCI CYCGRG 1 cut(s) 51
BslFI GGGAC 1 cut(s) 237
BslI CCNNNNNNNGG 1 cut(s) 19
BsmAI GTCTC 1 cut(s) 95
BsmFI GGGAC 1 cut(s) 237
BsnI GGCC 4 cut(s) 23, 65, 131, 206
BsoBI CYCGRG 1 cut(s) 51
Bsp1286I GDGCHC 1 cut(s) 58
Bsp143I GATC 2 cut(s) 124, 147
Bsp1720I GCTNAGC 1 cut(s) 224
BspACI CCGC 8 cut(s) 25, 42, 44, 62, 67, 111, 230, 283
BspANI GGCC 4 cut(s) 23, 65, 131, 206
BspFNI CGCG 5 cut(s) 27, 44, 69, 111, 230
BspLI GGNNCC 3 cut(s) 115, 155, 253
BspT107I GGYRCC 1 cut(s) 113
BsrI ACTGG 1 cut(s) 249
BssECI CCNNGG 2 cut(s) 42, 255
BssMI GATC 2 cut(s) 124, 147
BssSI CACGAG 1 cut(s) 57
BssT1I CCWWGG 1 cut(s) 255
Bst2BI CACGAG 1 cut(s) 57
Bst4CI ACNGT 1 cut(s) 251
Bst6I CTCTTC 1 cut(s) 111
BstC8I GCNNGC 3 cut(s) 25, 31, 67
BstDEI CTNAG 2 cut(s) 169, 224
BstDSI CCRYGG 1 cut(s) 42
BstFNI CGCG 5 cut(s) 27, 44, 69, 111, 230
BstHHI GCGC 2 cut(s) 29, 230
BstKTI GATC 2 cut(s) 127, 150
BstMAI GTCTC 1 cut(s) 95
BstMBI GATC 2 cut(s) 124, 147
BstMWI GCNNNNNNNGC 5 cut(s) 47, 62, 203, 236, 269
BstUI CGCG 5 cut(s) 27, 44, 69, 111, 230
BstV1I GCAGC 1 cut(s) 247
BstXI CCANNNNNNTGG 1 cut(s) 139
BsuRI GGCC 4 cut(s) 23, 65, 131, 206
BtgI CCRYGG 1 cut(s) 42
BtrI CACGTC 1 cut(s) 19
Cac8I GCNNGC 3 cut(s) 25, 31, 67
CfoI GCGC 2 cut(s) 29, 230
Cfr13I GGNCC 5 cut(s) 22, 64, 129, 205, 251
Cfr42I CCGCGG 1 cut(s) 45
Csp6I GTAC 1 cut(s) 74
CviAII CATG 2 cut(s) 139, 209
CviQI GTAC 1 cut(s) 74
DdeI CTNAG 2 cut(s) 169, 224
DpnI GATC 2 cut(s) 126, 149
DpnII GATC 2 cut(s) 124, 147
Eam1104I CTCTTC 1 cut(s) 111
EarI CTCTTC 1 cut(s) 111
Ecl136II GAGCTC 1 cut(s) 56
Eco130I CCWWGG 1 cut(s) 255
Eco24I GRGCYC 1 cut(s) 58
Eco47I GGWCC 1 cut(s) 251
Eco53kI GAGCTC 1 cut(s) 56
Eco88I CYCGRG 1 cut(s) 51
EcoICRI GAGCTC 1 cut(s) 56
EcoT14I CCWWGG 1 cut(s) 255
EcoT38I GRGCYC 1 cut(s) 58
ErhI CCWWGG 1 cut(s) 255
FaeI CATG 2 cut(s) 142, 212
FaiI YATR 2 cut(s) 140, 210
FalI AAGNNNNNCTT 2 cut(s) 152, 184
FaqI GGGAC 1 cut(s) 237
FatI CATG 2 cut(s) 138, 208
FauI CCCGC 2 cut(s) 32, 74
Fnu4HI GCNGC 5 cut(s) 42, 63, 112, 261, 283
FriOI GRGCYC 1 cut(s) 58
Fsp4HI GCNGC 5 cut(s) 42, 63, 112, 261, 283
FspBI CTAG 1 cut(s) 90
GlaI GCGC 2 cut(s) 28, 229
GluI GCNGC 5 cut(s) 42, 63, 112, 261, 283
GsuI CTGGAG 1 cut(s) 140
HaeIII GGCC 4 cut(s) 23, 65, 131, 206
HhaI GCGC 2 cut(s) 29, 230
Hin1II CATG 2 cut(s) 142, 212
Hin6I GCGC 2 cut(s) 27, 228
HinP1I GCGC 2 cut(s) 27, 228
HinfI GANTC 3 cut(s) 103, 181, 277
Hpy166II GTNNAC 1 cut(s) 214
Hpy188I TCNGA 2 cut(s) 96, 268
Hpy188III TCNNGA 1 cut(s) 157
Hpy8I GTNNAC 1 cut(s) 214
HpyCH4III ACNGT 1 cut(s) 251
HpyCH4IV ACGT 1 cut(s) 18
HpyF10VI GCNNNNNNNGC 5 cut(s) 47, 62, 203, 236, 269
HpyF3I CTNAG 2 cut(s) 169, 224
HpySE526I ACGT 1 cut(s) 18
Hsp92II CATG 2 cut(s) 142, 212
HspAI GCGC 2 cut(s) 27, 228
KspI CCGCGG 1 cut(s) 45
Kzo9I GATC 2 cut(s) 124, 147
LmnI GCTCC 1 cut(s) 159
LpnPI CCDG 4 cut(s) 136, 170, 191, 230
Lsp1109I GCAGC 1 cut(s) 247
MaeI CTAG 1 cut(s) 90
MaeII ACGT 1 cut(s) 18
MalI GATC 2 cut(s) 126, 149
MboI GATC 2 cut(s) 124, 147
MboII GAAGA 1 cut(s) 98
MhlI GDGCHC 1 cut(s) 58
MlyI GAGTC 2 cut(s) 97, 271
MnlI CCTC 3 cut(s) 40, 226, 274
MseI TTAA 1 cut(s) 293
MslI CAYNNNNRTG 1 cut(s) 137
MspA1I CMGCKG 1 cut(s) 44
MvnI CGCG 5 cut(s) 27, 44, 69, 111, 230
MwoI GCNNNNNNNGC 5 cut(s) 47, 62, 203, 236, 269
NdeII GATC 2 cut(s) 124, 147
NlaIII CATG 2 cut(s) 142, 212
NlaIV GGNNCC 3 cut(s) 115, 155, 253
PaeR7I CTCGAG 1 cut(s) 51
PfeI GAWTC 1 cut(s) 181
PkrI GCNGC 5 cut(s) 43, 64, 113, 262, 284
PleI GAGTC 2 cut(s) 97, 271
PpsI GAGTC 2 cut(s) 97, 271
Psp124BI GAGCTC 1 cut(s) 58
PspN4I GGNNCC 3 cut(s) 115, 155, 253
PspPI GGNCC 5 cut(s) 22, 64, 129, 205, 251
PspXI VCTCGAGB 1 cut(s) 51
RsaI GTAC 1 cut(s) 75
RsaNI GTAC 1 cut(s) 74
RseI CAYNNNNRTG 1 cut(s) 137
SacI GAGCTC 1 cut(s) 58
SacII CCGCGG 1 cut(s) 45
SaqAI TTAA 1 cut(s) 293
SatI GCNGC 5 cut(s) 42, 63, 112, 261, 283
Sau3AI GATC 2 cut(s) 124, 147
Sau96I GGNCC 5 cut(s) 22, 64, 129, 205, 251
SchI GAGTC 2 cut(s) 97, 271
SduI GDGCHC 1 cut(s) 58
SetI ASST 6 cut(s) 15, 21, 58, 119, 199, 225
Sfr274I CTCGAG 1 cut(s) 51
Sfr303I CCGCGG 1 cut(s) 45
SgrBI CCGCGG 1 cut(s) 45
SinI GGWCC 1 cut(s) 251
SlaI CTCGAG 1 cut(s) 51
SmiMI CAYNNNNRTG 1 cut(s) 137
SmlI CTYRAG 1 cut(s) 51
SmoI CTYRAG 1 cut(s) 51
SsiI CCGC 8 cut(s) 25, 42, 44, 62, 67, 111, 230, 283
SspMI CTAG 1 cut(s) 90
SstI GAGCTC 1 cut(s) 58
StyI CCWWGG 1 cut(s) 255
TaaI ACNGT 1 cut(s) 251
TaiI ACGT 1 cut(s) 21
TaqI TCGA 2 cut(s) 52, 184
TauI GCSGC 4 cut(s) 44, 65, 114, 285
TfiI GAWTC 1 cut(s) 181
Tru1I TTAA 1 cut(s) 293
Tru9I TTAA 1 cut(s) 293
TseI GCWGC 1 cut(s) 260
TspGWI ACGGA 1 cut(s) 91
VpaK11BI GGWCC 1 cut(s) 251
XhoI CTCGAG 1 cut(s) 51
XspI CTAG 1 cut(s) 90
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.