Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0004550

Physical Location & Seq

Genomic Coordinates Reverse (-)

20306470 .. 20308294

Gene Structure 1825 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1127800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 702 bp

ATGGGAAGGGAAATCGCCAAAAAATGTGCAGGTGTACCATTGGTGGCAAAGGTGTTGGGAAATATGATGTGTCGTAAACTGAGTGATGGATGGCAGTCAATTCAAGACAGTGCAATATGGGATTTGCCAGAAGGAGAAGAAAGAATATTTTTAGTTTTGAAGTTGAGTTTTGATGAATTGAGATCACCATCTTTGAAACAATGTTTTGCATATTGTTCAATGTTCGTCCAAGATGATATTATTTATAAGGAGGAATTAATCCAACTTTGGATGGCTCAAGGATTGCTCCAACCTTCTCACAACGAAAGTATGGAGGATGTAGGGAATGAAAATTTTAATATTCTACTGGAGAACTCCTTTTTTCAAGATGTTGAAATGAAGGGAAATACTGTTTACTATTGCAAGATGCACGATCTTATGCACAATCTTGCAGAACATGTGTCAAAATCACAGAGCAAGAACTTCCCTGAGATTCGACAGGAGGCAAAGCTCTCTACATCGGCACTGCATGTAATTCCAAACGGAGGTTTGAATGGTGAAATTCTCGGTGACATCTTATCAACATTTGAAGGTTTGCGCGTCTTAAAATTACGCGGTGCTGAAATTAGTGAGTTGTCAATTTCAATTGGTAAGTTGAAATACTTGAGGTATCTTGATGTTTCCTACACAAGGATCACAAAGCTCCCCAAATCTATCGGCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko03460 ko04014 map03460 map04014

Pfam Domains

Protein Families

NB-ARC

Protein Analysis

234

Amino Acids

26.41

Weight (kDa)

5.79

Isoelectric Point (pI)

50.37

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WHD_DRP	PF23559	74 - 144	3.7e-24	Disease resistance protein Winged helix domain
LRR_14	PF23598	191 - 232	4.2e-08	Leucine-rich repeat region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0004550)

Species	Orthologous Gene IDs
malus_domestica	MD12G1127500.v1.1 MD12G1127800.v1.1
pyrus_communis	pycom15g22880
rosa_chinensis	RchiOBHm_Chr3g0490741 RchiOBHm_Chr3g0490781 RchiOBHm_Chr5g0036381 RchiOBHm_Chr7g0204711
rosa_multiflora	Rmu_co8157836.1_g000001 Rmu_co8197550.1_g000002 Rmu_co8285827.1_g000001 Rmu_sc0000962.1_g000051 Rmu_sc0001049.1_g000003 Rmu_sc0002263.1_g000051 Rmu_sc0002489.1_g000041 Rmu_sc0004142.1_g000006 Rmu_sc0005425.1_g000014 Rmu_sc0037878.1_g000001
rosa_roxburghii	Rroxscaffold_2G00137800 Rroxscaffold_3G00248860 Rroxscaffold_5G00339360 Rroxscaffold_5G00378250 Rroxscaffold_6G00390190 Rroxscaffold_6G00393530 Rroxscaffold_7G00199500
rosa_samantha	Rh1BG194600 Rh3DG134500 Rh3DG149400 Rh3DG157200 Rh3DG157400 Rh4BG185400 Rh5CG399000
rosa_wichuraiana	Rw3G010890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	246
AarI	CACCTGC	1 cut(s)	20
Acc36I	ACCTGC	1 cut(s)	20
AccII	CGCG	2 cut(s)	579, 594
AciI	CCGC	1 cut(s)	594
AclWI	GGATC	1 cut(s)	680
AcsI	RAATTY	2 cut(s)	331, 540
AfaI	GTAC	1 cut(s)	36
AfiI	CCNNNNNNNGG	2 cut(s)	524, 669
AflIII	ACRYGT	1 cut(s)	436
AluBI	AGCT	2 cut(s)	490, 682
AluI	AGCT	2 cut(s)	490, 682
AlwI	GGATC	1 cut(s)	680
ApoI	RAATTY	2 cut(s)	331, 540
AseI	ATTAAT	1 cut(s)	257
AspLEI	GCGC	1 cut(s)	579
AsuHPI	GGTGA	3 cut(s)	177, 548, 560
BccI	CCATC	4 cut(s)	80, 84, 196, 265
BfaI	CTAG	1 cut(s)	700
BfuAI	ACCTGC	1 cut(s)	20
BmsI	GCATC	1 cut(s)	396
BpmI	CTGGAG	1 cut(s)	368
BpuEI	CTTGAG	2 cut(s)	261, 664
BsaBI	GATNNNNATC	1 cut(s)	187
Bsc4I	CCNNNNNNNGG	2 cut(s)	524, 669
Bse1I	ACTGG	1 cut(s)	351
Bse8I	GATNNNNATC	1 cut(s)	187
BseGI	GGATG	3 cut(s)	95, 276, 322
BseJI	GATNNNNATC	1 cut(s)	187
BseLI	CCNNNNNNNGG	2 cut(s)	524, 669
BseMII	CTCAG	2 cut(s)	71, 459
BseNI	ACTGG	1 cut(s)	351
BsgI	GTGCAG	1 cut(s)	48
Bsh1236I	CGCG	2 cut(s)	579, 594
BslI	CCNNNNNNNGG	2 cut(s)	524, 669
Bsp143I	GATC	3 cut(s)	182, 412, 672
BspACI	CCGC	1 cut(s)	594
BspCNI	CTCAG	2 cut(s)	72, 460
BspFNI	CGCG	2 cut(s)	579, 594
BspMI	ACCTGC	1 cut(s)	20
BspPI	GGATC	1 cut(s)	680
BsrI	ACTGG	1 cut(s)	351
BssMI	GATC	3 cut(s)	182, 412, 672
Bst4CI	ACNGT	2 cut(s)	110, 391
BstDEI	CTNAG	2 cut(s)	80, 468
BstENI	CCTNNNNNAGG	1 cut(s)	667
BstF5I	GGATG	3 cut(s)	95, 276, 322
BstFNI	CGCG	2 cut(s)	579, 594
BstHHI	GCGC	1 cut(s)	579
BstKTI	GATC	3 cut(s)	185, 415, 675
BstMBI	GATC	3 cut(s)	182, 412, 672
BstNSI	RCATGY	2 cut(s)	440, 512
BstUI	CGCG	2 cut(s)	579, 594
BtsCI	GGATG	3 cut(s)	95, 276, 322
BtsI	GCAGTG	1 cut(s)	503
BtsIMutI	CAGTG	2 cut(s)	115, 503
BveI	ACCTGC	1 cut(s)	20
CfoI	GCGC	1 cut(s)	579
CseI	GACGC	1 cut(s)	568
Csp6I	GTAC	1 cut(s)	35
CviAII	CATG	2 cut(s)	437, 509
CviJI	RGCY	4 cut(s)	275, 490, 682, 699
CviKI_1	RGCY	4 cut(s)	275, 490, 682, 699
CviQI	GTAC	1 cut(s)	35
DdeI	CTNAG	2 cut(s)	80, 468
DpnI	GATC	3 cut(s)	184, 414, 674
DpnII	GATC	3 cut(s)	182, 412, 672
EcoNI	CCTNNNNNAGG	1 cut(s)	667
FaeI	CATG	2 cut(s)	440, 512
FaiI	YATR	8 cut(s)	65, 118, 211, 246, 311, 419, 438, 510
FatI	CATG	2 cut(s)	436, 508
FokI	GGATG	3 cut(s)	102, 283, 329
FspBI	CTAG	1 cut(s)	700
GlaI	GCGC	1 cut(s)	578
GsuI	CTGGAG	1 cut(s)	368
HgaI	GACGC	1 cut(s)	568
HhaI	GCGC	1 cut(s)	579
Hin1II	CATG	2 cut(s)	440, 512
Hin6I	GCGC	1 cut(s)	577
HinP1I	GCGC	1 cut(s)	577
HinfI	GANTC	1 cut(s)	472
HphI	GGTGA	3 cut(s)	177, 548, 560
Hpy166II	GTNNAC	3 cut(s)	35, 77, 394
Hpy188III	TCNNGA	3 cut(s)	104, 365, 653
Hpy8I	GTNNAC	3 cut(s)	35, 77, 394
HpyAV	CCTTC	4 cut(s)	125, 303, 373, 563
HpyCH4III	ACNGT	2 cut(s)	110, 391
HpyCH4V	TGCA	8 cut(s)	29, 113, 209, 402, 409, 421, 431, 508
HpyF3I	CTNAG	2 cut(s)	80, 468
Hsp92II	CATG	2 cut(s)	440, 512
HspAI	GCGC	1 cut(s)	577
Kzo9I	GATC	3 cut(s)	182, 412, 672
LmnI	GCTCC	2 cut(s)	291, 687
LpnPI	CCDG	5 cut(s)	15, 141, 332, 464, 480
LweI	GCATC	1 cut(s)	396
MaeI	CTAG	1 cut(s)	700
MaeIII	GTNAC	1 cut(s)	548
MalI	GATC	3 cut(s)	184, 414, 674
MboI	GATC	3 cut(s)	182, 412, 672
MboII	GAAGA	1 cut(s)	149
MfeI	CAATTG	1 cut(s)	624
MmeI	TCCRAC	2 cut(s)	286, 313
MnlI	CCTC	5 cut(s)	244, 307, 475, 518, 639
MseI	TTAA	3 cut(s)	257, 336, 584
MunI	CAATTG	1 cut(s)	624
MvnI	CGCG	2 cut(s)	579, 594
NdeII	GATC	3 cut(s)	182, 412, 672
NlaIII	CATG	2 cut(s)	440, 512
NmuCI	GTSAC	1 cut(s)	548
NspI	RCATGY	2 cut(s)	440, 512
PaqCI	CACCTGC	1 cut(s)	20
PciI	ACATGT	1 cut(s)	436
PfeI	GAWTC	1 cut(s)	472
PscI	ACATGT	1 cut(s)	436
PshBI	ATTAAT	1 cut(s)	257
PsiI	TTATAA	1 cut(s)	246
RsaI	GTAC	1 cut(s)	36
RsaNI	GTAC	1 cut(s)	35
SaqAI	TTAA	3 cut(s)	257, 336, 584
Sau3AI	GATC	3 cut(s)	182, 412, 672
SetI	ASST	8 cut(s)	34, 54, 295, 492, 529, 574, 650, 684
SfaNI	GCATC	1 cut(s)	396
SmlI	CTYRAG	2 cut(s)	276, 643
SmoI	CTYRAG	2 cut(s)	276, 643
SsiI	CCGC	1 cut(s)	594
SspI	AATATT	2 cut(s)	147, 340
SspMI	CTAG	1 cut(s)	700
TaaI	ACNGT	2 cut(s)	110, 391
TaqI	TCGA	1 cut(s)	475
TfiI	GAWTC	1 cut(s)	472
Tru1I	TTAA	3 cut(s)	257, 336, 584
Tru9I	TTAA	3 cut(s)	257, 336, 584
TscAI	CASTG	2 cut(s)	115, 510
TseFI	GTSAC	1 cut(s)	548
Tsp45I	GTSAC	1 cut(s)	548
TspDTI	ATGAA	3 cut(s)	189, 342, 392
TspGWI	ACGGA	1 cut(s)	537
TspRI	CASTG	2 cut(s)	115, 510
VspI	ATTAAT	1 cut(s)	257
XagI	CCTNNNNNAGG	1 cut(s)	667
XapI	RAATTY	2 cut(s)	331, 540
XceI	RCATGY	2 cut(s)	440, 512
XspI	CTAG	1 cut(s)	700

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.