MD14G1041500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0003126
Physical Location & Seq
Reverse (-)
3943577 .. 3945115
1539 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1041500.v1.1.491

Sequence Viewer

Length: 732 bp
ATGGCGGTGATTTCTCCATGGACGACGACCTTGATGATCACGATTATGATGATAACGACGGTCATGCATGTAATCGACGGCAGCGACAACAACCCCGTGTACTCGCCGTGCGCGGACACGAAGGTGGAGAGGTCCGACGGGTTCACCTTCGGAATTGCCTTCGCCTCCAAGGAATCCTTCCAACGAAACGGCACCGTTCAGCTGTCTCCTTGCGACTCCAGACTTTCTCTCTCCTCCAATGCCCAGATCTCCGTTTTCCGACCCAAAGTTGACGAGATCTCCCTCCTCTCCGTCAACTCCTCCTCCTTCAATCCGGATACTTATGGGTTTATGGTTGCGTTTGCTGGGCGAAAATATGCTGCAAGGTCCCTTCCTACTTTTGTTGCTAATGGTACCTACACTGTCACCAGCTTTACCCTTGTGCTTGAGTTCAACAAGGGCAGGCTGCAGAACTTGTACTGGAAAAAGGATGGGTGCTCTAAATGTTCAGGGAGCTCCGACTTTGTTTGCCTCAACAATCAGCACTGCGCCTTCAAGACCTCGAGCTGCAAAAACCATGGCGGCTCTGTGGACTGCAGCCTCGGAATCCAGTTGGCATTTTCCGGCACAGACAAGCACCTTTCGGTTCTTAACTCATGGTATGAAGTGGCAAACCTTAGACAGTACTCGCTCTATGGTCTTTATTCGAATTTGAAGGACTCCCTCAGTAGCCAGTACAGCAAGATTTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

244

Amino Acids

26.76

Weight (kDa)

8.58

Isoelectric Point (pI)

28.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 392
AccB1I GGYRCC 2 cut(s) 191, 392
AccII CGCG 1 cut(s) 113
AccIII TCCGGA 1 cut(s) 313
AciI CCGC 3 cut(s) 5, 113, 561
AcsI RAATTY 1 cut(s) 688
AfaI GTAC 5 cut(s) 101, 394, 458, 665, 716
AgsI TTSAA 4 cut(s) 310, 433, 535, 694
AjuI GAANNNNNNNTTGG 2 cut(s) 161, 193
AleI CACNNNNGTG 1 cut(s) 122
AluBI AGCT 4 cut(s) 202, 411, 495, 546
AluI AGCT 4 cut(s) 202, 411, 495, 546
Alw21I GWGCWC 2 cut(s) 479, 497
Alw26I GTCTC 1 cut(s) 210
Ama87I CYCGRG 1 cut(s) 541
Aor13HI TCCGGA 1 cut(s) 313
ApeKI GCWGC 5 cut(s) 81, 359, 445, 546, 576
ApoI RAATTY 1 cut(s) 688
Asp718I GGTACC 1 cut(s) 392
AspLEI GCGC 2 cut(s) 113, 530
AspS9I GGNCC 2 cut(s) 132, 366
AsuHPI GGTGA 3 cut(s) 19, 136, 397
AsuII TTCGAA 1 cut(s) 686
AvaI CYCGRG 1 cut(s) 541
AvaII GGWCC 2 cut(s) 132, 366
BanI GGYRCC 2 cut(s) 191, 392
BanII GRGCYC 1 cut(s) 497
Bbv12I GWGCWC 2 cut(s) 479, 497
BbvI GCAGC 5 cut(s) 93, 346, 432, 533, 588
BccI CCATC 1 cut(s) 464
BceAI ACGGC 3 cut(s) 91, 94, 205
BcgI CGANNNNNNTGC 2 cut(s) 46, 80
BciVI GTATCC 1 cut(s) 310
BclI TGATCA 1 cut(s) 36
BcoDI GTCTC 1 cut(s) 210
BfmI CTRYAG 2 cut(s) 446, 574
BfuI GTATCC 1 cut(s) 310
BglII AGATCT 2 cut(s) 246, 276
BisI GCNGC 6 cut(s) 82, 360, 446, 547, 562, 577
BlsI GCNGC 6 cut(s) 83, 361, 447, 548, 563, 578
BmcAI AGTACT 1 cut(s) 665
Bme18I GGWCC 2 cut(s) 132, 366
BmeT110I CYCGRG 1 cut(s) 541
BmgT120I GGNCC 2 cut(s) 132, 366
BmiI GGNNCC 3 cut(s) 193, 368, 394
BpmI CTGGAG 1 cut(s) 202
Bpu14I TTCGAA 1 cut(s) 686
BpuEI CTTGAG 1 cut(s) 446
BsaJI CCNNGG 4 cut(s) 17, 168, 556, 580
BsaWI WCCGGW 1 cut(s) 313
BsaXI ACNNNNNCTCC 4 cut(s) 263, 287, 293, 317
Bse1I ACTGG 3 cut(s) 464, 589, 712
BseAI TCCGGA 1 cut(s) 313
BseDI CCNNGG 4 cut(s) 17, 168, 556, 580
BseGI GGATG 1 cut(s) 475
BseMII CTCAG 1 cut(s) 718
BseNI ACTGG 3 cut(s) 464, 589, 712
BseRI GAGGAG 4 cut(s) 223, 275, 289, 292
BseXI GCAGC 5 cut(s) 93, 346, 432, 533, 588
BseYI CCCAGC 1 cut(s) 344
Bsh1236I CGCG 1 cut(s) 113
BshNI GGYRCC 2 cut(s) 191, 392
BsiHKAI GWGCWC 2 cut(s) 479, 497
BsiHKCI CYCGRG 1 cut(s) 541
BsiSI CCGG 2 cut(s) 314, 603
BslFI GGGAC 1 cut(s) 352
BsmAI GTCTC 1 cut(s) 210
BsmFI GGGAC 1 cut(s) 352
BsoBI CYCGRG 1 cut(s) 541
Bsp119I TTCGAA 1 cut(s) 686
Bsp1286I GDGCHC 2 cut(s) 479, 497
Bsp13I TCCGGA 1 cut(s) 313
Bsp143I GATC 3 cut(s) 36, 246, 276
Bsp19I CCATGG 2 cut(s) 17, 556
BspACI CCGC 3 cut(s) 5, 113, 561
BspCNI CTCAG 1 cut(s) 717
BspEI TCCGGA 1 cut(s) 313
BspFNI CGCG 1 cut(s) 113
BspLI GGNNCC 3 cut(s) 193, 368, 394
BspMAI CTGCAG 2 cut(s) 450, 578
BspT104I TTCGAA 1 cut(s) 686
BspT107I GGYRCC 2 cut(s) 191, 392
BsrI ACTGG 3 cut(s) 464, 589, 712
BssECI CCNNGG 4 cut(s) 17, 168, 556, 580
BssMI GATC 3 cut(s) 36, 246, 276
BssT1I CCWWGG 3 cut(s) 17, 168, 556
Bst4CI ACNGT 4 cut(s) 61, 196, 403, 663
BstBI TTCGAA 1 cut(s) 686
BstC8I GCNNGC 1 cut(s) 443
BstDEI CTNAG 2 cut(s) 656, 704
BstDSI CCRYGG 2 cut(s) 17, 556
BstF5I GGATG 1 cut(s) 475
BstFNI CGCG 1 cut(s) 113
BstHHI GCGC 2 cut(s) 113, 530
BstKTI GATC 3 cut(s) 39, 249, 279
BstMAI GTCTC 1 cut(s) 210
BstMBI GATC 3 cut(s) 36, 246, 276
BstMWI GCNNNNNNNGC 1 cut(s) 717
BstNSI RCATGY 1 cut(s) 71
BstSFI CTRYAG 2 cut(s) 446, 574
BstUI CGCG 1 cut(s) 113
BstV1I GCAGC 5 cut(s) 93, 346, 432, 533, 588
BstX2I RGATCY 2 cut(s) 246, 276
BstYI RGATCY 2 cut(s) 246, 276
BsuI GTATCC 1 cut(s) 310
BtgI CCRYGG 2 cut(s) 17, 556
BtsCI GGATG 1 cut(s) 475
BtsI GCAGTG 1 cut(s) 523
BtsIMutI CAGTG 2 cut(s) 399, 523
Cac8I GCNNGC 1 cut(s) 443
CfoI GCGC 2 cut(s) 113, 530
Cfr13I GGNCC 2 cut(s) 132, 366
Csp6I GTAC 5 cut(s) 100, 393, 457, 664, 715
CviAII CATG 5 cut(s) 18, 64, 68, 557, 636
CviJI RGCY 8 cut(s) 202, 411, 445, 495, 546, 564, 579, 711
CviKI_1 RGCY 8 cut(s) 202, 411, 445, 495, 546, 564, 579, 711
CviQI GTAC 5 cut(s) 100, 393, 457, 664, 715
DdeI CTNAG 2 cut(s) 656, 704
DpnI GATC 3 cut(s) 38, 248, 278
DpnII GATC 3 cut(s) 36, 246, 276
Ecl136II GAGCTC 1 cut(s) 495
Eco130I CCWWGG 3 cut(s) 17, 168, 556
Eco24I GRGCYC 1 cut(s) 497
Eco47I GGWCC 2 cut(s) 132, 366
Eco53kI GAGCTC 1 cut(s) 495
Eco88I CYCGRG 1 cut(s) 541
EcoICRI GAGCTC 1 cut(s) 495
EcoO109I RGGNCCY 1 cut(s) 366
EcoT14I CCWWGG 3 cut(s) 17, 168, 556
EcoT22I ATGCAT 1 cut(s) 69
EcoT38I GRGCYC 1 cut(s) 497
ErhI CCWWGG 3 cut(s) 17, 168, 556
FaeI CATG 5 cut(s) 21, 67, 71, 560, 639
FalI AAGNNNNNCTT 2 cut(s) 161, 193
FaqI GGGAC 1 cut(s) 352
FatI CATG 5 cut(s) 17, 63, 67, 556, 635
FbaI TGATCA 1 cut(s) 36
Fnu4HI GCNGC 6 cut(s) 82, 360, 446, 547, 562, 577
FokI GGATG 1 cut(s) 482
FriOI GRGCYC 1 cut(s) 497
Fsp4HI GCNGC 6 cut(s) 82, 360, 446, 547, 562, 577
GlaI GCGC 2 cut(s) 112, 529
GluI GCNGC 6 cut(s) 82, 360, 446, 547, 562, 577
GsaI CCCAGC 1 cut(s) 348
GsuI CTGGAG 1 cut(s) 202
HapII CCGG 2 cut(s) 314, 603
HhaI GCGC 2 cut(s) 113, 530
Hin1II CATG 5 cut(s) 21, 67, 71, 560, 639
Hin6I GCGC 2 cut(s) 111, 528
HinP1I GCGC 2 cut(s) 111, 528
HincII GTYRAC 2 cut(s) 271, 295
HindII GTYRAC 2 cut(s) 271, 295
HinfI GANTC 4 cut(s) 173, 215, 585, 698
HpaII CCGG 2 cut(s) 314, 603
HphI GGTGA 3 cut(s) 19, 136, 397
Hpy166II GTNNAC 5 cut(s) 100, 144, 271, 295, 571
Hpy188I TCNGA 5 cut(s) 136, 152, 260, 499, 584
Hpy188III TCNNGA 4 cut(s) 40, 219, 314, 535
Hpy8I GTNNAC 5 cut(s) 100, 144, 271, 295, 571
Hpy99I CGWCG 4 cut(s) 28, 61, 80, 140
HpyAV CCTTC 8 cut(s) 115, 157, 169, 187, 316, 380, 541, 688
HpyCH4III ACNGT 4 cut(s) 61, 196, 403, 663
HpyCH4V TGCA 5 cut(s) 67, 362, 448, 549, 576
HpyF10VI GCNNNNNNNGC 1 cut(s) 717
HpyF3I CTNAG 2 cut(s) 656, 704
Hsp92II CATG 5 cut(s) 21, 67, 71, 560, 639
HspAI GCGC 2 cut(s) 111, 528
Kpn2I TCCGGA 1 cut(s) 313
KpnI GGTACC 1 cut(s) 396
Ksp22I TGATCA 1 cut(s) 36
Kzo9I GATC 3 cut(s) 36, 246, 276
LmnI GCTCC 2 cut(s) 492, 500
Lsp1109I GCAGC 5 cut(s) 93, 346, 432, 533, 588
MaeIII GTNAC 1 cut(s) 403
MalI GATC 3 cut(s) 38, 248, 278
MboI GATC 3 cut(s) 36, 246, 276
MflI RGATCY 2 cut(s) 246, 276
MhlI GDGCHC 2 cut(s) 479, 497
MluCI AATT 2 cut(s) 153, 688
MlyI GAGTC 2 cut(s) 209, 692
MmeI TCCRAC 4 cut(s) 159, 205, 283, 522
Mph1103I ATGCAT 1 cut(s) 69
MroI TCCGGA 1 cut(s) 313
MseI TTAA 1 cut(s) 630
MslI CAYNNNNRTG 2 cut(s) 44, 122
MspA1I CMGCKG 1 cut(s) 202
MspI CCGG 2 cut(s) 314, 603
MvnI CGCG 1 cut(s) 113
MwoI GCNNNNNNNGC 1 cut(s) 717
NcoI CCATGG 2 cut(s) 17, 556
NdeII GATC 3 cut(s) 36, 246, 276
NlaIII CATG 5 cut(s) 21, 67, 71, 560, 639
NlaIV GGNNCC 3 cut(s) 193, 368, 394
NmuCI GTSAC 1 cut(s) 403
NsiI ATGCAT 1 cut(s) 69
NspI RCATGY 1 cut(s) 71
NspV TTCGAA 1 cut(s) 686
OliI CACNNNNGTG 1 cut(s) 122
PaeR7I CTCGAG 1 cut(s) 541
PcsI WCGNNNNNNNCGW 1 cut(s) 81
PfeI GAWTC 2 cut(s) 173, 585
PkrI GCNGC 6 cut(s) 83, 361, 447, 548, 563, 578
PleI GAGTC 2 cut(s) 209, 692
PpsI GAGTC 2 cut(s) 209, 692
PpuMI RGGWCCY 1 cut(s) 366
Psp124BI GAGCTC 1 cut(s) 497
Psp5II RGGWCCY 1 cut(s) 366
PspFI CCCAGC 1 cut(s) 344
PspN4I GGNNCC 3 cut(s) 193, 368, 394
PspPI GGNCC 2 cut(s) 132, 366
PspPPI RGGWCCY 1 cut(s) 366
PspXI VCTCGAGB 1 cut(s) 541
PstI CTGCAG 2 cut(s) 450, 578
PsuI RGATCY 2 cut(s) 246, 276
PvuII CAGCTG 1 cut(s) 202
RsaI GTAC 5 cut(s) 101, 394, 458, 665, 716
RsaNI GTAC 5 cut(s) 100, 393, 457, 664, 715
RseI CAYNNNNRTG 2 cut(s) 44, 122
SacI GAGCTC 1 cut(s) 497
SaqAI TTAA 1 cut(s) 630
SatI GCNGC 6 cut(s) 82, 360, 446, 547, 562, 577
Sau3AI GATC 3 cut(s) 36, 246, 276
Sau96I GGNCC 2 cut(s) 132, 366
ScaI AGTACT 1 cut(s) 665
SchI GAGTC 2 cut(s) 209, 692
SduI GDGCHC 2 cut(s) 479, 497
SfcI CTRYAG 2 cut(s) 446, 574
Sfr274I CTCGAG 1 cut(s) 541
SfuI TTCGAA 1 cut(s) 686
SinI GGWCC 2 cut(s) 132, 366
SlaI CTCGAG 1 cut(s) 541
SmiMI CAYNNNNRTG 2 cut(s) 44, 122
SmlI CTYRAG 2 cut(s) 425, 541
SmoI CTYRAG 2 cut(s) 425, 541
Sse9I AATT 2 cut(s) 153, 688
SsiI CCGC 3 cut(s) 5, 113, 561
SstI GAGCTC 1 cut(s) 497
StyI CCWWGG 3 cut(s) 17, 168, 556
TaaI ACNGT 4 cut(s) 61, 196, 403, 663
TaqI TCGA 3 cut(s) 75, 542, 686
TasI AATT 2 cut(s) 153, 688
TatI WGTACW 4 cut(s) 99, 456, 663, 714
TauI GCSGC 1 cut(s) 564
TfiI GAWTC 2 cut(s) 173, 585
Tru1I TTAA 1 cut(s) 630
Tru9I TTAA 1 cut(s) 630
TscAI CASTG 2 cut(s) 406, 530
TseFI GTSAC 1 cut(s) 403
TseI GCWGC 5 cut(s) 81, 359, 445, 546, 576
Tsp45I GTSAC 1 cut(s) 403
TspDTI ATGAA 1 cut(s) 657
TspGWI ACGGA 2 cut(s) 241, 280
TspRI CASTG 2 cut(s) 406, 530
VpaK11BI GGWCC 2 cut(s) 132, 366
XapI RAATTY 1 cut(s) 688
XceI RCATGY 1 cut(s) 71
XhoI CTCGAG 1 cut(s) 541
ZrmI AGTACT 1 cut(s) 665
Zsp2I ATGCAT 1 cut(s) 69
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.