Rw2G013950

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0003126
Physical Location & Seq
Forward (+)
15169475 .. 15176166
6692 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G013950.1

Sequence Viewer

Length: 456 bp
ATGGTTGCTTTTGCTGGGCATAAATATGCTGCAAGGTCTACTGCGGCTTTTGTTGCCAATAGTACGTACACTGTCACCAGCTTTACTCTTGTGCTTGAGTTCACCAAGGGCAGGCTGGAACACCTGCATTGGAAAAGGGATGGGTGCGTCAAATGTTCTGGGAGCTCCAGGTTCGTTTTCCTGCACAATCGGGACTGTGCAATCAAGACAAACAGCTGCAAAACCCATGGAGGCACTGTGGATTGCAGTCTCAGGATCCAATTGGCATTTTCTGGGACGGATAATCACCTATCCATTCTGAACTCATGGTATGAAGCACAGAACCTTGGTCAGTACTCCCTTTATGTTTTACAGGCAAGTGATCAAGTTGATTTGTTCCTCTTACCAGGAGTAGCATTTGACAGACCTGGGAGACGTTTGGCTTGCGACAGAGGTGGAGCAAAATGGACCCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

151

Amino Acids

16.75

Weight (kDa)

9.15

Isoelectric Point (pI)

29.07

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 132
Acc36I ACCTGC 1 cut(s) 132
AccI GTMKAC 1 cut(s) 38
AciI CCGC 1 cut(s) 44
AclWI GGATC 2 cut(s) 250, 263
AfaI GTAC 3 cut(s) 64, 68, 335
AfiI CCNNNNNNNGG 1 cut(s) 111
AjnI CCWGG 3 cut(s) 167, 385, 406
AluBI AGCT 3 cut(s) 81, 165, 216
AluI AGCT 3 cut(s) 81, 165, 216
Alw21I GWGCWC 1 cut(s) 167
Alw26I GTCTC 2 cut(s) 254, 406
AlwI GGATC 2 cut(s) 250, 263
ApeKI GCWGC 2 cut(s) 29, 216
AspS9I GGNCC 1 cut(s) 447
AsuHPI GGTGA 3 cut(s) 67, 94, 278
AvaII GGWCC 1 cut(s) 447
BamHI GGATCC 1 cut(s) 255
BanII GRGCYC 1 cut(s) 167
Bbv12I GWGCWC 1 cut(s) 167
BbvI GCAGC 2 cut(s) 16, 203
BccI CCATC 1 cut(s) 134
BciT130I CCWGG 3 cut(s) 169, 387, 408
BclI TGATCA 1 cut(s) 361
BcoDI GTCTC 2 cut(s) 254, 406
BfuAI ACCTGC 1 cut(s) 132
BisI GCNGC 3 cut(s) 30, 45, 217
BlsI GCNGC 3 cut(s) 31, 46, 218
BmcAI AGTACT 1 cut(s) 335
Bme1390I CCNGG 3 cut(s) 169, 387, 408
Bme18I GGWCC 1 cut(s) 447
BmgT120I GGNCC 1 cut(s) 447
BmiI GGNNCC 2 cut(s) 257, 449
BmrFI CCNGG 3 cut(s) 169, 387, 408
BpmI CTGGAG 1 cut(s) 151
BpuEI CTTGAG 1 cut(s) 116
BsaAI YACGTR 1 cut(s) 66
BsaJI CCNNGG 4 cut(s) 105, 226, 325, 407
BsaXI ACNNNNNCTCC 2 cut(s) 222, 252
Bsc4I CCNNNNNNNGG 1 cut(s) 111
BseBI CCWGG 3 cut(s) 169, 387, 408
BseDI CCNNGG 4 cut(s) 105, 226, 325, 407
BseGI GGATG 1 cut(s) 145
BseLI CCNNNNNNNGG 1 cut(s) 111
BseMII CTCAG 1 cut(s) 265
BseXI GCAGC 2 cut(s) 16, 203
BseYI CCCAGC 1 cut(s) 14
BsgI GTGCAG 1 cut(s) 167
BsiHKAI GWGCWC 1 cut(s) 167
BslFI GGGAC 2 cut(s) 206, 289
BslI CCNNNNNNNGG 1 cut(s) 111
BsmAI GTCTC 2 cut(s) 254, 406
BsmBI CGTCTC 1 cut(s) 406
BsmFI GGGAC 2 cut(s) 206, 289
Bsp1286I GDGCHC 1 cut(s) 167
Bsp143I GATC 2 cut(s) 255, 361
Bsp19I CCATGG 1 cut(s) 226
BspACI CCGC 1 cut(s) 44
BspCNI CTCAG 1 cut(s) 264
BspLI GGNNCC 2 cut(s) 257, 449
BspMI ACCTGC 1 cut(s) 132
BspPI GGATC 2 cut(s) 250, 263
BssECI CCNNGG 4 cut(s) 105, 226, 325, 407
BssMI GATC 2 cut(s) 255, 361
BssT1I CCWWGG 3 cut(s) 105, 226, 325
Bst2UI CCWGG 3 cut(s) 169, 387, 408
Bst4CI ACNGT 3 cut(s) 73, 197, 238
BstBAI YACGTR 1 cut(s) 66
BstC8I GCNNGC 2 cut(s) 113, 424
BstDEI CTNAG 1 cut(s) 251
BstDSI CCRYGG 1 cut(s) 226
BstF5I GGATG 1 cut(s) 145
BstKTI GATC 2 cut(s) 258, 364
BstMAI GTCTC 2 cut(s) 254, 406
BstMBI GATC 2 cut(s) 255, 361
BstMWI GCNNNNNNNGC 1 cut(s) 53
BstNI CCWGG 3 cut(s) 169, 387, 408
BstSCI CCNGG 3 cut(s) 167, 385, 406
BstSNI TACGTA 1 cut(s) 66
BstV1I GCAGC 2 cut(s) 16, 203
BstX2I RGATCY 1 cut(s) 255
BstYI RGATCY 1 cut(s) 255
BtgI CCRYGG 1 cut(s) 226
BtsCI GGATG 1 cut(s) 145
BtsIMutI CAGTG 2 cut(s) 69, 234
BveI ACCTGC 1 cut(s) 132
Cac8I GCNNGC 2 cut(s) 113, 424
Cfr13I GGNCC 1 cut(s) 447
CseI GACGC 1 cut(s) 136
Csp6I GTAC 3 cut(s) 63, 67, 334
CviAII CATG 2 cut(s) 227, 306
CviJI RGCY 6 cut(s) 47, 81, 115, 165, 216, 422
CviKI_1 RGCY 6 cut(s) 47, 81, 115, 165, 216, 422
CviQI GTAC 3 cut(s) 63, 67, 334
DdeI CTNAG 1 cut(s) 251
DpnI GATC 2 cut(s) 257, 363
DpnII GATC 2 cut(s) 255, 361
Ecl136II GAGCTC 1 cut(s) 165
Eco105I TACGTA 1 cut(s) 66
Eco130I CCWWGG 3 cut(s) 105, 226, 325
Eco24I GRGCYC 1 cut(s) 167
Eco47I GGWCC 1 cut(s) 447
Eco53kI GAGCTC 1 cut(s) 165
EcoICRI GAGCTC 1 cut(s) 165
EcoRII CCWGG 3 cut(s) 167, 385, 406
EcoT14I CCWWGG 3 cut(s) 105, 226, 325
EcoT38I GRGCYC 1 cut(s) 167
ErhI CCWWGG 3 cut(s) 105, 226, 325
Esp3I CGTCTC 1 cut(s) 406
FaeI CATG 2 cut(s) 230, 309
FaiI YATR 6 cut(s) 21, 27, 228, 307, 312, 345
FaqI GGGAC 2 cut(s) 206, 289
FatI CATG 2 cut(s) 226, 305
FbaI TGATCA 1 cut(s) 361
FblI GTMKAC 1 cut(s) 38
Fnu4HI GCNGC 3 cut(s) 30, 45, 217
FokI GGATG 1 cut(s) 152
FriOI GRGCYC 1 cut(s) 167
Fsp4HI GCNGC 3 cut(s) 30, 45, 217
GluI GCNGC 3 cut(s) 30, 45, 217
GsaI CCCAGC 1 cut(s) 18
GsuI CTGGAG 1 cut(s) 151
HgaI GACGC 1 cut(s) 136
Hin1II CATG 2 cut(s) 230, 309
HphI GGTGA 3 cut(s) 67, 94, 278
Hpy166II GTNNAC 3 cut(s) 39, 69, 102
Hpy188I TCNGA 1 cut(s) 300
Hpy188III TCNNGA 3 cut(s) 191, 205, 253
Hpy8I GTNNAC 3 cut(s) 39, 69, 102
HpyCH4III ACNGT 3 cut(s) 73, 197, 238
HpyCH4IV ACGT 2 cut(s) 65, 415
HpyCH4V TGCA 6 cut(s) 32, 127, 184, 200, 219, 246
HpyF10VI GCNNNNNNNGC 1 cut(s) 53
HpyF3I CTNAG 1 cut(s) 251
HpySE526I ACGT 2 cut(s) 65, 415
Hsp92II CATG 2 cut(s) 230, 309
Ksp22I TGATCA 1 cut(s) 361
Kzo9I GATC 2 cut(s) 255, 361
LmnI GCTCC 3 cut(s) 162, 170, 437
Lsp1109I GCAGC 2 cut(s) 16, 203
MaeII ACGT 2 cut(s) 65, 415
MaeIII GTNAC 1 cut(s) 73
MalI GATC 2 cut(s) 257, 363
MboI GATC 2 cut(s) 255, 361
MfeI CAATTG 1 cut(s) 260
MflI RGATCY 1 cut(s) 255
MhlI GDGCHC 1 cut(s) 167
MluCI AATT 1 cut(s) 260
MnlI CCTC 3 cut(s) 224, 389, 425
MslI CAYNNNNRTG 1 cut(s) 24
MspA1I CMGCKG 1 cut(s) 216
MspR9I CCNGG 3 cut(s) 169, 387, 408
MunI CAATTG 1 cut(s) 260
MvaI CCWGG 3 cut(s) 169, 387, 408
MwoI GCNNNNNNNGC 1 cut(s) 53
NcoI CCATGG 1 cut(s) 226
NdeII GATC 2 cut(s) 255, 361
NlaIII CATG 2 cut(s) 230, 309
NlaIV GGNNCC 2 cut(s) 257, 449
NmuCI GTSAC 1 cut(s) 73
PaqCI CACCTGC 1 cut(s) 132
PkrI GCNGC 3 cut(s) 31, 46, 218
Ppu21I YACGTR 1 cut(s) 66
Psp124BI GAGCTC 1 cut(s) 167
Psp6I CCWGG 3 cut(s) 167, 385, 406
PspFI CCCAGC 1 cut(s) 14
PspGI CCWGG 3 cut(s) 167, 385, 406
PspN4I GGNNCC 2 cut(s) 257, 449
PspPI GGNCC 1 cut(s) 447
PsuI RGATCY 1 cut(s) 255
PvuII CAGCTG 1 cut(s) 216
RsaI GTAC 3 cut(s) 64, 68, 335
RsaNI GTAC 3 cut(s) 63, 67, 334
RseI CAYNNNNRTG 1 cut(s) 24
SacI GAGCTC 1 cut(s) 167
SatI GCNGC 3 cut(s) 30, 45, 217
Sau3AI GATC 2 cut(s) 255, 361
Sau96I GGNCC 1 cut(s) 447
ScaI AGTACT 1 cut(s) 335
ScrFI CCNGG 3 cut(s) 169, 387, 408
SduI GDGCHC 1 cut(s) 167
SinI GGWCC 1 cut(s) 447
SmiMI CAYNNNNRTG 1 cut(s) 24
SmlI CTYRAG 1 cut(s) 95
SmoI CTYRAG 1 cut(s) 95
SnaBI TACGTA 1 cut(s) 66
Sse9I AATT 1 cut(s) 260
SsiI CCGC 1 cut(s) 44
SstI GAGCTC 1 cut(s) 167
StyD4I CCNGG 3 cut(s) 167, 385, 406
StyI CCWWGG 3 cut(s) 105, 226, 325
TaaI ACNGT 3 cut(s) 73, 197, 238
TaiI ACGT 2 cut(s) 68, 418
TasI AATT 1 cut(s) 260
TatI WGTACW 1 cut(s) 333
TauI GCSGC 1 cut(s) 47
TscAI CASTG 2 cut(s) 76, 241
TseFI GTSAC 1 cut(s) 73
TseI GCWGC 2 cut(s) 29, 216
Tsp45I GTSAC 1 cut(s) 73
TspDTI ATGAA 1 cut(s) 327
TspGWI ACGGA 1 cut(s) 293
TspRI CASTG 2 cut(s) 76, 241
VpaK11BI GGWCC 1 cut(s) 447
XcmI CCANNNNNNNNNTGG 1 cut(s) 112
XmiI GTMKAC 1 cut(s) 38
ZrmI AGTACT 1 cut(s) 335
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.