RchiOBHm_Chr4g0411221

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0003126
Physical Location & Seq
Forward (+)
35007156 .. 35007662
507 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ38205

Sequence Viewer

Length: 402 bp
ATGGTTGCTTTTGCTGGGCATAAATATGCTGCAAGGTCTACTGCGGCTTTTGTTGCCAATAGTACCTACACTGTCACCAGCTTTACTCTTGTGCTTGAGTTCACCGAGGGCAGGCTGCAGCACCTGCATTGGAAAAGGGATGGGTGCGTCAAATGTTCTGGGAGCTCCAGGTTCATTTGCCTGCACAATCGGGACTGTGCAATCAAGACAAACATCTACAAAACCCATGGAGGCACTGTGGATTGCAGTCTCAGGATCCAATTGGCATTTTCTGGGACGGATAAGCACCTATCCGTTCTGAACTCATGGTATGAAGCACAGAACCTTGGTCAGTACTCCCTTTATGGTCTGTATTCAAATCTGAAGGATTCTCTCACTAGCCAGTATAACAAGTTTTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

133

Amino Acids

14.99

Weight (kDa)

9.16

Isoelectric Point (pI)

28.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 132
Acc36I ACCTGC 1 cut(s) 132
AccI GTMKAC 1 cut(s) 38
AciI CCGC 1 cut(s) 44
AclWI GGATC 2 cut(s) 250, 263
AcuI CTGAAG 1 cut(s) 383
AfaI GTAC 2 cut(s) 64, 335
AfiI CCNNNNNNNGG 1 cut(s) 111
AgsI TTSAA 1 cut(s) 357
AjnI CCWGG 1 cut(s) 167
AluBI AGCT 2 cut(s) 81, 165
AluI AGCT 2 cut(s) 81, 165
Alw21I GWGCWC 1 cut(s) 167
Alw26I GTCTC 1 cut(s) 254
AlwI GGATC 2 cut(s) 250, 263
AlwNI CAGNNNCTG 1 cut(s) 124
ApeKI GCWGC 3 cut(s) 29, 115, 118
AsuHPI GGTGA 2 cut(s) 67, 94
BamHI GGATCC 1 cut(s) 255
BanII GRGCYC 1 cut(s) 167
Bbv12I GWGCWC 1 cut(s) 167
BbvI GCAGC 3 cut(s) 16, 102, 130
BccI CCATC 1 cut(s) 134
BciT130I CCWGG 1 cut(s) 169
BcoDI GTCTC 1 cut(s) 254
BfaI CTAG 1 cut(s) 378
BfmI CTRYAG 1 cut(s) 116
BfuAI ACCTGC 1 cut(s) 132
BisI GCNGC 4 cut(s) 30, 45, 116, 119
BlsI GCNGC 4 cut(s) 31, 46, 117, 120
BmcAI AGTACT 1 cut(s) 335
Bme1390I CCNGG 1 cut(s) 169
BmiI GGNNCC 1 cut(s) 257
BmrFI CCNGG 1 cut(s) 169
BpmI CTGGAG 1 cut(s) 151
BpuEI CTTGAG 1 cut(s) 116
BsaJI CCNNGG 3 cut(s) 105, 226, 325
BsaXI ACNNNNNCTCC 2 cut(s) 222, 252
Bsc4I CCNNNNNNNGG 1 cut(s) 111
Bse1I ACTGG 1 cut(s) 382
BseBI CCWGG 1 cut(s) 169
BseDI CCNNGG 3 cut(s) 105, 226, 325
BseGI GGATG 1 cut(s) 145
BseLI CCNNNNNNNGG 1 cut(s) 111
BseMII CTCAG 1 cut(s) 265
BseNI ACTGG 1 cut(s) 382
BseXI GCAGC 3 cut(s) 16, 102, 130
BseYI CCCAGC 1 cut(s) 14
BsgI GTGCAG 1 cut(s) 167
BsiHKAI GWGCWC 1 cut(s) 167
BslFI GGGAC 2 cut(s) 206, 289
BslI CCNNNNNNNGG 1 cut(s) 111
BsmAI GTCTC 1 cut(s) 254
BsmFI GGGAC 2 cut(s) 206, 289
Bsp1286I GDGCHC 1 cut(s) 167
Bsp143I GATC 1 cut(s) 255
Bsp19I CCATGG 1 cut(s) 226
BspACI CCGC 1 cut(s) 44
BspCNI CTCAG 1 cut(s) 264
BspLI GGNNCC 1 cut(s) 257
BspMAI CTGCAG 1 cut(s) 120
BspMI ACCTGC 1 cut(s) 132
BspPI GGATC 2 cut(s) 250, 263
BsrI ACTGG 1 cut(s) 382
BssECI CCNNGG 3 cut(s) 105, 226, 325
BssMI GATC 1 cut(s) 255
BssT1I CCWWGG 2 cut(s) 226, 325
Bst2UI CCWGG 1 cut(s) 169
Bst4CI ACNGT 3 cut(s) 73, 197, 238
BstAPI GCANNNNNTGC 1 cut(s) 124
BstC8I GCNNGC 2 cut(s) 113, 182
BstDEI CTNAG 1 cut(s) 251
BstDSI CCRYGG 1 cut(s) 226
BstF5I GGATG 1 cut(s) 145
BstKTI GATC 1 cut(s) 258
BstMAI GTCTC 1 cut(s) 254
BstMBI GATC 1 cut(s) 255
BstMWI GCNNNNNNNGC 2 cut(s) 53, 124
BstNI CCWGG 1 cut(s) 169
BstSCI CCNGG 1 cut(s) 167
BstSFI CTRYAG 1 cut(s) 116
BstV1I GCAGC 3 cut(s) 16, 102, 130
BstX2I RGATCY 1 cut(s) 255
BstYI RGATCY 1 cut(s) 255
BtgI CCRYGG 1 cut(s) 226
BtsCI GGATG 1 cut(s) 145
BtsIMutI CAGTG 2 cut(s) 69, 234
BveI ACCTGC 1 cut(s) 132
Cac8I GCNNGC 2 cut(s) 113, 182
CaiI CAGNNNCTG 1 cut(s) 124
CseI GACGC 1 cut(s) 136
Csp6I GTAC 2 cut(s) 63, 334
CviAII CATG 2 cut(s) 227, 306
CviJI RGCY 5 cut(s) 47, 81, 115, 165, 381
CviKI_1 RGCY 5 cut(s) 47, 81, 115, 165, 381
CviQI GTAC 2 cut(s) 63, 334
DdeI CTNAG 1 cut(s) 251
DpnI GATC 1 cut(s) 257
DpnII GATC 1 cut(s) 255
Ecl136II GAGCTC 1 cut(s) 165
Eco130I CCWWGG 2 cut(s) 226, 325
Eco24I GRGCYC 1 cut(s) 167
Eco53kI GAGCTC 1 cut(s) 165
Eco57I CTGAAG 1 cut(s) 383
EcoICRI GAGCTC 1 cut(s) 165
EcoRII CCWGG 1 cut(s) 167
EcoT14I CCWWGG 2 cut(s) 226, 325
EcoT38I GRGCYC 1 cut(s) 167
ErhI CCWWGG 2 cut(s) 226, 325
FaeI CATG 2 cut(s) 230, 309
FaiI YATR 7 cut(s) 21, 27, 228, 307, 312, 345, 387
FaqI GGGAC 2 cut(s) 206, 289
FatI CATG 2 cut(s) 226, 305
FblI GTMKAC 1 cut(s) 38
Fnu4HI GCNGC 4 cut(s) 30, 45, 116, 119
FokI GGATG 1 cut(s) 152
FriOI GRGCYC 1 cut(s) 167
Fsp4HI GCNGC 4 cut(s) 30, 45, 116, 119
FspBI CTAG 1 cut(s) 378
GluI GCNGC 4 cut(s) 30, 45, 116, 119
GsaI CCCAGC 1 cut(s) 18
GsuI CTGGAG 1 cut(s) 151
HgaI GACGC 1 cut(s) 136
Hin1II CATG 2 cut(s) 230, 309
HinfI GANTC 1 cut(s) 368
HphI GGTGA 2 cut(s) 67, 94
Hpy166II GTNNAC 2 cut(s) 39, 102
Hpy188I TCNGA 3 cut(s) 300, 363, 401
Hpy188III TCNNGA 3 cut(s) 191, 205, 253
Hpy8I GTNNAC 2 cut(s) 39, 102
HpyAV CCTTC 1 cut(s) 358
HpyCH4III ACNGT 3 cut(s) 73, 197, 238
HpyCH4V TGCA 6 cut(s) 32, 118, 127, 184, 200, 246
HpyF10VI GCNNNNNNNGC 2 cut(s) 53, 124
HpyF3I CTNAG 1 cut(s) 251
Hsp92II CATG 2 cut(s) 230, 309
Kzo9I GATC 1 cut(s) 255
LmnI GCTCC 2 cut(s) 162, 170
Lsp1109I GCAGC 3 cut(s) 16, 102, 130
MaeI CTAG 1 cut(s) 378
MaeIII GTNAC 1 cut(s) 73
MalI GATC 1 cut(s) 257
MboI GATC 1 cut(s) 255
MfeI CAATTG 1 cut(s) 260
MflI RGATCY 1 cut(s) 255
MhlI GDGCHC 1 cut(s) 167
MluCI AATT 1 cut(s) 260
MnlI CCTC 2 cut(s) 100, 224
MslI CAYNNNNRTG 1 cut(s) 24
MspR9I CCNGG 1 cut(s) 169
MunI CAATTG 1 cut(s) 260
MvaI CCWGG 1 cut(s) 169
MwoI GCNNNNNNNGC 2 cut(s) 53, 124
NcoI CCATGG 1 cut(s) 226
NdeII GATC 1 cut(s) 255
NlaIII CATG 2 cut(s) 230, 309
NlaIV GGNNCC 1 cut(s) 257
NmuCI GTSAC 1 cut(s) 73
PaqCI CACCTGC 1 cut(s) 132
PfeI GAWTC 1 cut(s) 368
PkrI GCNGC 4 cut(s) 31, 46, 117, 120
Psp124BI GAGCTC 1 cut(s) 167
Psp6I CCWGG 1 cut(s) 167
PspFI CCCAGC 1 cut(s) 14
PspGI CCWGG 1 cut(s) 167
PspN4I GGNNCC 1 cut(s) 257
PstI CTGCAG 1 cut(s) 120
PstNI CAGNNNCTG 1 cut(s) 124
PsuI RGATCY 1 cut(s) 255
RsaI GTAC 2 cut(s) 64, 335
RsaNI GTAC 2 cut(s) 63, 334
RseI CAYNNNNRTG 1 cut(s) 24
SacI GAGCTC 1 cut(s) 167
SatI GCNGC 4 cut(s) 30, 45, 116, 119
Sau3AI GATC 1 cut(s) 255
ScaI AGTACT 1 cut(s) 335
ScrFI CCNGG 1 cut(s) 169
SduI GDGCHC 1 cut(s) 167
SetI ASST 8 cut(s) 38, 68, 83, 126, 167, 173, 291, 327
SfcI CTRYAG 1 cut(s) 116
SmiMI CAYNNNNRTG 1 cut(s) 24
SmlI CTYRAG 1 cut(s) 95
SmoI CTYRAG 1 cut(s) 95
Sse9I AATT 1 cut(s) 260
SsiI CCGC 1 cut(s) 44
SspMI CTAG 1 cut(s) 378
SstI GAGCTC 1 cut(s) 167
StyD4I CCNGG 1 cut(s) 167
StyI CCWWGG 2 cut(s) 226, 325
TaaI ACNGT 3 cut(s) 73, 197, 238
TasI AATT 1 cut(s) 260
TatI WGTACW 1 cut(s) 333
TauI GCSGC 1 cut(s) 47
TfiI GAWTC 1 cut(s) 368
TscAI CASTG 2 cut(s) 76, 241
TseFI GTSAC 1 cut(s) 73
TseI GCWGC 3 cut(s) 29, 115, 118
Tsp45I GTSAC 1 cut(s) 73
TspDTI ATGAA 2 cut(s) 163, 327
TspGWI ACGGA 2 cut(s) 283, 293
TspRI CASTG 2 cut(s) 76, 241
XmiI GTMKAC 1 cut(s) 38
XspI CTAG 1 cut(s) 378
ZrmI AGTACT 1 cut(s) 335
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.