Rh6AG328100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0003126
Physical Location & Seq
Forward (+)
53022770 .. 53026287
3518 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG328100.1

Sequence Viewer

Length: 546 bp
ATGGTTGCTTTTGCTGGGCATAAATATGCTGCAAGGTCTACTGCGGCTTTTGTTGCCAATAGTACCTACACTGTCACCAGCTTTACTCTTGTGCTTGAGTTCACCGAGGGCAGGCTGCAGCACCTGTATTGGAAAAGGGATGGGTGCGTCAAATGTTCTGGGAGCTCCAGGTTCGTTTGCCTGCACAATCGGGACTGTGCAATCAAGACAAACAGCTGCAAAACCCATGGAGGCACTGTGGATTGCAGTCTTAGGATCCAATTGGCATTTTCTGGGACGGATAAGCACCTATCCGTTCTGAACTCATGGTATGAAGCACAGAACCTTGGTCAGTACTCCCTTTATGTTTTACAGGCAAGTGATCAAGTTGATTTGTTCCTCTTACCAGGAGCAGCATTTGACAGACCTGGGAGACGTTTGGCTTGCGACATAGGCAAATCTTGCTCAAGTGAGGGAGGCAAATCAAGGCAGATCAAGCAAAAAGACAAGAAATGCAACTTGCAGAGCGAGTTGATGGCTGCAAAATCTACTGCAGCTCAGCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

181

Amino Acids

19.89

Weight (kDa)

9.19

Isoelectric Point (pI)

43.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 38
AciI CCGC 1 cut(s) 44
AclWI GGATC 2 cut(s) 250, 263
AfaI GTAC 2 cut(s) 64, 335
AfiI CCNNNNNNNGG 1 cut(s) 111
AjnI CCWGG 3 cut(s) 167, 385, 406
AluBI AGCT 4 cut(s) 81, 165, 216, 536
AluI AGCT 4 cut(s) 81, 165, 216, 536
Alw21I GWGCWC 1 cut(s) 167
Alw26I GTCTC 1 cut(s) 406
AlwI GGATC 2 cut(s) 250, 263
AlwNI CAGNNNCTG 1 cut(s) 124
ApeKI GCWGC 7 cut(s) 29, 115, 118, 216, 392, 518, 533
AsuHPI GGTGA 2 cut(s) 67, 94
BamHI GGATCC 1 cut(s) 255
BanII GRGCYC 1 cut(s) 167
Bbv12I GWGCWC 1 cut(s) 167
BbvI GCAGC 6 cut(s) 16, 102, 130, 203, 404, 505
BccI CCATC 2 cut(s) 134, 508
BciT130I CCWGG 3 cut(s) 169, 387, 408
BclI TGATCA 1 cut(s) 361
BcoDI GTCTC 1 cut(s) 406
BfmI CTRYAG 2 cut(s) 116, 531
BisI GCNGC 8 cut(s) 30, 45, 116, 119, 217, 393, 519, 534
BlpI GCTNAGC 1 cut(s) 537
BlsI GCNGC 8 cut(s) 31, 46, 117, 120, 218, 394, 520, 535
BmcAI AGTACT 1 cut(s) 335
Bme1390I CCNGG 3 cut(s) 169, 387, 408
BmiI GGNNCC 1 cut(s) 257
BmrFI CCNGG 3 cut(s) 169, 387, 408
BpmI CTGGAG 1 cut(s) 151
Bpu1102I GCTNAGC 1 cut(s) 537
BpuEI CTTGAG 2 cut(s) 116, 430
BsaJI CCNNGG 4 cut(s) 105, 226, 325, 407
BsaXI ACNNNNNCTCC 2 cut(s) 222, 252
Bsc4I CCNNNNNNNGG 1 cut(s) 111
BseBI CCWGG 3 cut(s) 169, 387, 408
BseDI CCNNGG 4 cut(s) 105, 226, 325, 407
BseGI GGATG 1 cut(s) 145
BseLI CCNNNNNNNGG 1 cut(s) 111
BseXI GCAGC 6 cut(s) 16, 102, 130, 203, 404, 505
BseYI CCCAGC 1 cut(s) 14
BsgI GTGCAG 1 cut(s) 167
BsiHKAI GWGCWC 1 cut(s) 167
BslFI GGGAC 2 cut(s) 206, 289
BslI CCNNNNNNNGG 1 cut(s) 111
BsmAI GTCTC 1 cut(s) 406
BsmBI CGTCTC 1 cut(s) 406
BsmFI GGGAC 2 cut(s) 206, 289
Bsp1286I GDGCHC 1 cut(s) 167
Bsp143I GATC 3 cut(s) 255, 361, 471
Bsp1720I GCTNAGC 1 cut(s) 537
Bsp19I CCATGG 1 cut(s) 226
BspACI CCGC 1 cut(s) 44
BspLI GGNNCC 1 cut(s) 257
BspMAI CTGCAG 2 cut(s) 120, 535
BspPI GGATC 2 cut(s) 250, 263
BssECI CCNNGG 4 cut(s) 105, 226, 325, 407
BssMI GATC 3 cut(s) 255, 361, 471
BssT1I CCWWGG 2 cut(s) 226, 325
Bst2UI CCWGG 3 cut(s) 169, 387, 408
Bst4CI ACNGT 3 cut(s) 73, 197, 238
BstAPI GCANNNNNTGC 1 cut(s) 441
BstC8I GCNNGC 3 cut(s) 113, 182, 424
BstDEI CTNAG 2 cut(s) 251, 537
BstDSI CCRYGG 1 cut(s) 226
BstF5I GGATG 1 cut(s) 145
BstKTI GATC 3 cut(s) 258, 364, 474
BstMAI GTCTC 1 cut(s) 406
BstMBI GATC 3 cut(s) 255, 361, 471
BstMWI GCNNNNNNNGC 4 cut(s) 53, 432, 441, 475
BstNI CCWGG 3 cut(s) 169, 387, 408
BstSCI CCNGG 3 cut(s) 167, 385, 406
BstSFI CTRYAG 2 cut(s) 116, 531
BstV1I GCAGC 6 cut(s) 16, 102, 130, 203, 404, 505
BstX2I RGATCY 1 cut(s) 255
BstYI RGATCY 1 cut(s) 255
BtgI CCRYGG 1 cut(s) 226
BtsCI GGATG 1 cut(s) 145
BtsIMutI CAGTG 2 cut(s) 69, 234
Cac8I GCNNGC 3 cut(s) 113, 182, 424
CaiI CAGNNNCTG 1 cut(s) 124
CseI GACGC 1 cut(s) 136
Csp6I GTAC 2 cut(s) 63, 334
CviAII CATG 2 cut(s) 227, 306
CviJI RGCY 8 cut(s) 47, 81, 115, 165, 216, 422, 518, 536
CviKI_1 RGCY 8 cut(s) 47, 81, 115, 165, 216, 422, 518, 536
CviQI GTAC 2 cut(s) 63, 334
DdeI CTNAG 2 cut(s) 251, 537
DpnI GATC 3 cut(s) 257, 363, 473
DpnII GATC 3 cut(s) 255, 361, 471
Ecl136II GAGCTC 1 cut(s) 165
Eco130I CCWWGG 2 cut(s) 226, 325
Eco24I GRGCYC 1 cut(s) 167
Eco53kI GAGCTC 1 cut(s) 165
EcoICRI GAGCTC 1 cut(s) 165
EcoRII CCWGG 3 cut(s) 167, 385, 406
EcoT14I CCWWGG 2 cut(s) 226, 325
EcoT38I GRGCYC 1 cut(s) 167
ErhI CCWWGG 2 cut(s) 226, 325
Esp3I CGTCTC 1 cut(s) 406
FaeI CATG 2 cut(s) 230, 309
FaiI YATR 7 cut(s) 21, 27, 228, 307, 312, 345, 431
FaqI GGGAC 2 cut(s) 206, 289
FatI CATG 2 cut(s) 226, 305
FbaI TGATCA 1 cut(s) 361
FblI GTMKAC 1 cut(s) 38
Fnu4HI GCNGC 8 cut(s) 30, 45, 116, 119, 217, 393, 519, 534
FokI GGATG 1 cut(s) 152
FriOI GRGCYC 1 cut(s) 167
Fsp4HI GCNGC 8 cut(s) 30, 45, 116, 119, 217, 393, 519, 534
GluI GCNGC 8 cut(s) 30, 45, 116, 119, 217, 393, 519, 534
GsaI CCCAGC 1 cut(s) 18
GsuI CTGGAG 1 cut(s) 151
HgaI GACGC 1 cut(s) 136
Hin1II CATG 2 cut(s) 230, 309
HphI GGTGA 2 cut(s) 67, 94
Hpy166II GTNNAC 2 cut(s) 39, 102
Hpy188I TCNGA 1 cut(s) 300
Hpy188III TCNNGA 2 cut(s) 191, 205
Hpy8I GTNNAC 2 cut(s) 39, 102
HpyCH4III ACNGT 3 cut(s) 73, 197, 238
HpyCH4IV ACGT 1 cut(s) 415
HpyF10VI GCNNNNNNNGC 4 cut(s) 53, 432, 441, 475
HpyF3I CTNAG 2 cut(s) 251, 537
HpySE526I ACGT 1 cut(s) 415
Hsp92II CATG 2 cut(s) 230, 309
Ksp22I TGATCA 1 cut(s) 361
Kzo9I GATC 3 cut(s) 255, 361, 471
LmnI GCTCC 3 cut(s) 162, 170, 389
Lsp1109I GCAGC 6 cut(s) 16, 102, 130, 203, 404, 505
MaeII ACGT 1 cut(s) 415
MaeIII GTNAC 1 cut(s) 73
MalI GATC 3 cut(s) 257, 363, 473
MboI GATC 3 cut(s) 255, 361, 471
MfeI CAATTG 1 cut(s) 260
MflI RGATCY 1 cut(s) 255
MhlI GDGCHC 1 cut(s) 167
MluCI AATT 1 cut(s) 260
MnlI CCTC 5 cut(s) 100, 224, 389, 445, 449
MslI CAYNNNNRTG 1 cut(s) 24
MspA1I CMGCKG 1 cut(s) 216
MspR9I CCNGG 3 cut(s) 169, 387, 408
MunI CAATTG 1 cut(s) 260
MvaI CCWGG 3 cut(s) 169, 387, 408
MwoI GCNNNNNNNGC 4 cut(s) 53, 432, 441, 475
NcoI CCATGG 1 cut(s) 226
NdeII GATC 3 cut(s) 255, 361, 471
NlaIII CATG 2 cut(s) 230, 309
NlaIV GGNNCC 1 cut(s) 257
NmuCI GTSAC 1 cut(s) 73
PkrI GCNGC 8 cut(s) 31, 46, 117, 120, 218, 394, 520, 535
Psp124BI GAGCTC 1 cut(s) 167
Psp6I CCWGG 3 cut(s) 167, 385, 406
PspFI CCCAGC 1 cut(s) 14
PspGI CCWGG 3 cut(s) 167, 385, 406
PspN4I GGNNCC 1 cut(s) 257
PstI CTGCAG 2 cut(s) 120, 535
PstNI CAGNNNCTG 1 cut(s) 124
PsuI RGATCY 1 cut(s) 255
PvuII CAGCTG 1 cut(s) 216
RsaI GTAC 2 cut(s) 64, 335
RsaNI GTAC 2 cut(s) 63, 334
RseI CAYNNNNRTG 1 cut(s) 24
SacI GAGCTC 1 cut(s) 167
SatI GCNGC 8 cut(s) 30, 45, 116, 119, 217, 393, 519, 534
Sau3AI GATC 3 cut(s) 255, 361, 471
ScaI AGTACT 1 cut(s) 335
ScrFI CCNGG 3 cut(s) 169, 387, 408
SduI GDGCHC 1 cut(s) 167
SfcI CTRYAG 2 cut(s) 116, 531
SmiMI CAYNNNNRTG 1 cut(s) 24
SmlI CTYRAG 2 cut(s) 95, 445
SmoI CTYRAG 2 cut(s) 95, 445
Sse9I AATT 1 cut(s) 260
SsiI CCGC 1 cut(s) 44
SstI GAGCTC 1 cut(s) 167
StyD4I CCNGG 3 cut(s) 167, 385, 406
StyI CCWWGG 2 cut(s) 226, 325
TaaI ACNGT 3 cut(s) 73, 197, 238
TaiI ACGT 1 cut(s) 418
TasI AATT 1 cut(s) 260
TatI WGTACW 1 cut(s) 333
TauI GCSGC 1 cut(s) 47
TscAI CASTG 2 cut(s) 76, 241
TseFI GTSAC 1 cut(s) 73
TseI GCWGC 7 cut(s) 29, 115, 118, 216, 392, 518, 533
Tsp45I GTSAC 1 cut(s) 73
TspDTI ATGAA 1 cut(s) 327
TspGWI ACGGA 2 cut(s) 283, 293
TspRI CASTG 2 cut(s) 76, 241
XmiI GTMKAC 1 cut(s) 38
ZrmI AGTACT 1 cut(s) 335
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.