MD14G1069400.v1.1
NAC Family

NAC domain-containing protein 67-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0002868
Physical Location & Seq
Forward (+)
7601377 .. 7603555
2179 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1069400.v1.1.491

Sequence Viewer

Length: 1014 bp
ATGGTTCCAGTTCTCAATGAAGTAGGTCCAGTTGATGCATATATTGATTCTCTTCCACCGGGTTATCGATTTGTCCCTACCGACGATGAATTGATTTTACACTACTTGATGAGAAAGGTTTTGAATGAGCAGCTTCCCATTAACAGGTTTCGTATTGTTAATCTCTACGATCACCATCCTCGAGACCTTACCGATTACAATTTGATTAGAGAGAGTGAGTGGTACTTTTTCACTTCAAGGAGACGGAAGTACCCGAGAGGAGGACGGCCATGTCGAGCAGCAGATAATGGATACTGGAAGGCAACTGGCAAGCCTGATGACATTAAAGATGATAATGGCCAAGTAATTGGGTCTAAAAGGACTCTAGATTATTATGAAGGGAAACAAGGAAAAGGGACAAAGACGGACTGGAAAATGCATGAATATAAGCTCAATAAAGAGACCGCTCCTTCTAACCAAATTGATGGTACTAACATGAAGTTAGATGATTGTGTTTTGTGTAAGATTTACAAGAATAGCAGAATCAAGAAGGATGGGAAGAACAACGACGACAATGCACCTCAGAGTACTATCAGAACTGATCAAGGAGCTATACTGCCTGCAGCTACAAATACTGTAACATCTCATGCTTTTCCAGATCAATCAATTCAACATCCGTATCTTTTTACTGGTGAATCTAGTGGCACTACTTCTTCCATTGTAAATAAACATTGTGTTACGTCAACCTCGACAACACCGGCATATTATGAGCAGCATTATTTCCAAAATCAAACATGGTCCGATCATCCAGGACCAATCCACAACTGCAACAATCAAATTCCACCGCCAAGGGAGCCATTTTCTTCTCAATTTCCACTGCCAAATTCAATGGAACATCAGATTGGGGCAGGATGGGAGTCTGTTAATGGAATGCCAGTGGTTGATTCGAGTTCTATTCGGTTACTTCCCCATGTCAGGGAATATGAACGTGAGCTTAAGGCTATGAGAAACAACGAAGGCCAGGGAACGTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAM

Protein Analysis

338

Amino Acids

38.54

Weight (kDa)

6.86

Isoelectric Point (pI)

35.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 19 - 144 8.3e-41 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 270
AccBSI CCGCTC 1 cut(s) 446
AciI CCGC 2 cut(s) 444, 824
AcoI YGGCCR 2 cut(s) 266, 337
AcsI RAATTY 2 cut(s) 816, 862
AfaI GTAC 4 cut(s) 224, 251, 469, 568
AfiI CCNNNNNNNGG 4 cut(s) 144, 260, 954, 955
AflII CTTAAG 1 cut(s) 974
AgsI TTSAA 4 cut(s) 124, 237, 650, 867
AjnI CCWGG 2 cut(s) 787, 999
AjuI GAANNNNNNNTTGG 2 cut(s) 864, 896
AluBI AGCT 5 cut(s) 133, 430, 590, 605, 973
AluI AGCT 5 cut(s) 133, 430, 590, 605, 973
Alw26I GTCTC 3 cut(s) 177, 235, 434
Ama87I CYCGRG 2 cut(s) 180, 253
AoxI GGCC 3 cut(s) 266, 337, 997
ApeKI GCWGC 4 cut(s) 130, 278, 602, 751
ApoI RAATTY 2 cut(s) 816, 862
AspS9I GGNCC 3 cut(s) 26, 777, 791
AsuC2I CCSGG 1 cut(s) 60
AsuHPI GGTGA 2 cut(s) 164, 683
AvaI CYCGRG 2 cut(s) 180, 253
AvaII GGWCC 3 cut(s) 26, 777, 791
BaeI ACNNNNGTAYC 4 cut(s) 233, 266, 641, 674
BalI TGGCCA 1 cut(s) 339
BbvI GCAGC 4 cut(s) 142, 290, 614, 763
BccI CCATC 4 cut(s) 183, 458, 527, 885
BceAI ACGGC 1 cut(s) 281
BcgI CGANNNNNNTGC 2 cut(s) 536, 570
BciT130I CCWGG 2 cut(s) 789, 1001
BciVI GTATCC 1 cut(s) 284
BclI TGATCA 1 cut(s) 580
BcnI CCSGG 1 cut(s) 60
BcoDI GTCTC 3 cut(s) 177, 235, 434
BfaI CTAG 2 cut(s) 365, 678
BfmI CTRYAG 1 cut(s) 600
BfrI CTTAAG 1 cut(s) 974
BfuI GTATCC 1 cut(s) 284
BisI GCNGC 4 cut(s) 131, 279, 603, 752
BlsI GCNGC 4 cut(s) 132, 280, 604, 753
BmcAI AGTACT 1 cut(s) 568
Bme1390I CCNGG 3 cut(s) 60, 789, 1001
Bme18I GGWCC 3 cut(s) 26, 777, 791
BmeT110I CYCGRG 2 cut(s) 180, 253
BmgT120I GGNCC 3 cut(s) 26, 777, 791
BmiI GGNNCC 2 cut(s) 6, 834
BmrFI CCNGG 3 cut(s) 60, 789, 1001
BmsI GCATC 1 cut(s) 25
BpuMI CCSGG 1 cut(s) 60
Bsa29I ATCGAT 1 cut(s) 67
BsaBI GATNNNNATC 1 cut(s) 174
BsaI GGTCTC 2 cut(s) 177, 434
BsaJI CCNNGG 2 cut(s) 827, 1000
Bsc4I CCNNNNNNNGG 4 cut(s) 144, 260, 954, 955
Bse118I RCCGGY 1 cut(s) 736
Bse1I ACTGG 7 cut(s) 8, 29, 299, 310, 413, 673, 914
Bse8I GATNNNNATC 1 cut(s) 174
BseBI CCWGG 2 cut(s) 789, 1001
BseCI ATCGAT 1 cut(s) 67
BseDI CCNNGG 2 cut(s) 827, 1000
BseGI GGATG 5 cut(s) 175, 538, 652, 784, 896
BseJI GATNNNNATC 1 cut(s) 174
BseLI CCNNNNNNNGG 4 cut(s) 144, 260, 954, 955
BseMII CTCAG 1 cut(s) 575
BseNI ACTGG 7 cut(s) 8, 29, 299, 310, 413, 673, 914
BseRI GAGGAG 1 cut(s) 273
BseXI GCAGC 4 cut(s) 142, 290, 614, 763
BshFI GGCC 3 cut(s) 268, 339, 999
BshVI ATCGAT 1 cut(s) 67
BsiHKCI CYCGRG 2 cut(s) 180, 253
BsiSI CCGG 2 cut(s) 59, 737
BslFI GGGAC 2 cut(s) 59, 409
BslI CCNNNNNNNGG 4 cut(s) 144, 260, 954, 955
BsmAI GTCTC 3 cut(s) 177, 235, 434
BsmBI CGTCTC 1 cut(s) 235
BsmFI GGGAC 2 cut(s) 59, 409
BsmI GAATGC 1 cut(s) 915
BsnI GGCC 3 cut(s) 268, 339, 999
Bso31I GGTCTC 2 cut(s) 177, 434
BsoBI CYCGRG 2 cut(s) 180, 253
Bsp143I GATC 4 cut(s) 169, 580, 637, 781
BspACI CCGC 2 cut(s) 444, 824
BspANI GGCC 3 cut(s) 268, 339, 999
BspCNI CTCAG 1 cut(s) 574
BspDI ATCGAT 1 cut(s) 67
BspLI GGNNCC 2 cut(s) 6, 834
BspMAI CTGCAG 1 cut(s) 604
BspTI CTTAAG 1 cut(s) 974
BspTNI GGTCTC 2 cut(s) 177, 434
BsrBI CCGCTC 1 cut(s) 446
BsrFI RCCGGY 1 cut(s) 736
BsrI ACTGG 7 cut(s) 8, 29, 299, 310, 413, 673, 914
BssAI RCCGGY 1 cut(s) 736
BssECI CCNNGG 2 cut(s) 827, 1000
BssMI GATC 4 cut(s) 169, 580, 637, 781
BssT1I CCWWGG 1 cut(s) 827
Bst2UI CCWGG 2 cut(s) 789, 1001
Bst4CI ACNGT 1 cut(s) 616
Bst6I CTCTTC 1 cut(s) 57
BstAFI CTTAAG 1 cut(s) 974
BstC8I GCNNGC 2 cut(s) 311, 600
BstDEI CTNAG 1 cut(s) 561
BstF5I GGATG 5 cut(s) 175, 538, 652, 784, 896
BstKTI GATC 4 cut(s) 172, 583, 640, 784
BstMAI GTCTC 3 cut(s) 177, 235, 434
BstMBI GATC 4 cut(s) 169, 580, 637, 781
BstMWI GCNNNNNNNGC 1 cut(s) 832
BstNI CCWGG 2 cut(s) 789, 1001
BstSCI CCNGG 3 cut(s) 58, 787, 999
BstSFI CTRYAG 1 cut(s) 600
BstV1I GCAGC 4 cut(s) 142, 290, 614, 763
BstXI CCANNNNNNTGG 2 cut(s) 347, 464
Bsu15I ATCGAT 1 cut(s) 67
BsuI GTATCC 1 cut(s) 284
BsuRI GGCC 3 cut(s) 268, 339, 999
BsuTUI ATCGAT 1 cut(s) 67
BtsCI GGATG 5 cut(s) 175, 538, 652, 784, 896
BtsI GCAGTG 1 cut(s) 854
BtsIMutI CAGTG 2 cut(s) 854, 921
Cac8I GCNNGC 2 cut(s) 311, 600
Cfr10I RCCGGY 1 cut(s) 736
Cfr13I GGNCC 3 cut(s) 26, 777, 791
ClaI ATCGAT 1 cut(s) 67
Csp6I GTAC 4 cut(s) 223, 250, 468, 567
CviAII CATG 6 cut(s) 270, 419, 475, 626, 774, 950
CviQI GTAC 4 cut(s) 223, 250, 468, 567
DdeI CTNAG 1 cut(s) 561
DpnI GATC 4 cut(s) 171, 582, 639, 783
DpnII GATC 4 cut(s) 169, 580, 637, 781
DrdI GACNNNNNNGTC 1 cut(s) 270
DseDI GACNNNNNNGTC 1 cut(s) 270
EaeI YGGCCR 2 cut(s) 266, 337
Eam1104I CTCTTC 1 cut(s) 57
EarI CTCTTC 1 cut(s) 57
Eco130I CCWWGG 1 cut(s) 827
Eco31I GGTCTC 2 cut(s) 177, 434
Eco47I GGWCC 3 cut(s) 26, 777, 791
Eco88I CYCGRG 2 cut(s) 180, 253
EcoRII CCWGG 2 cut(s) 787, 999
EcoT14I CCWWGG 1 cut(s) 827
EcoT22I ATGCAT 2 cut(s) 40, 420
ErhI CCWWGG 1 cut(s) 827
Esp3I CGTCTC 1 cut(s) 235
FaeI CATG 6 cut(s) 273, 422, 478, 629, 777, 953
FaqI GGGAC 2 cut(s) 59, 409
FatI CATG 6 cut(s) 269, 418, 474, 625, 773, 949
FbaI TGATCA 1 cut(s) 580
Fnu4HI GCNGC 4 cut(s) 131, 279, 603, 752
FokI GGATG 5 cut(s) 162, 545, 639, 771, 903
Fsp4HI GCNGC 4 cut(s) 131, 279, 603, 752
FspBI CTAG 2 cut(s) 365, 678
GluI GCNGC 4 cut(s) 131, 279, 603, 752
HaeIII GGCC 3 cut(s) 268, 339, 999
HapII CCGG 2 cut(s) 59, 737
Hin1II CATG 6 cut(s) 273, 422, 478, 629, 777, 953
HincII GTYRAC 1 cut(s) 723
HindII GTYRAC 1 cut(s) 723
HinfI GANTC 6 cut(s) 47, 361, 522, 674, 896, 923
HpaII CCGG 2 cut(s) 59, 737
HphI GGTGA 2 cut(s) 164, 683
Hpy166II GTNNAC 1 cut(s) 723
Hpy188I TCNGA 4 cut(s) 564, 575, 781, 879
Hpy188III TCNNGA 4 cut(s) 182, 365, 526, 635
Hpy8I GTNNAC 1 cut(s) 723
Hpy99I CGWCG 2 cut(s) 86, 551
HpyAV CCTTC 5 cut(s) 292, 371, 459, 523, 989
HpyCH4III ACNGT 1 cut(s) 616
HpyCH4IV ACGT 3 cut(s) 719, 967, 1007
HpyCH4V TGCA 5 cut(s) 38, 418, 557, 602, 807
HpyF10VI GCNNNNNNNGC 1 cut(s) 832
HpyF3I CTNAG 1 cut(s) 561
HpySE526I ACGT 3 cut(s) 719, 967, 1007
Hsp92II CATG 6 cut(s) 273, 422, 478, 629, 777, 953
Ksp22I TGATCA 1 cut(s) 580
Kzo9I GATC 4 cut(s) 169, 580, 637, 781
LmnI GCTCC 3 cut(s) 451, 587, 832
Lsp1109I GCAGC 4 cut(s) 142, 290, 614, 763
LweI GCATC 1 cut(s) 25
MaeI CTAG 2 cut(s) 365, 678
MaeII ACGT 3 cut(s) 719, 967, 1007
MaeIII GTNAC 3 cut(s) 616, 715, 939
MalI GATC 4 cut(s) 171, 582, 639, 783
MbiI CCGCTC 1 cut(s) 446
MboI GATC 4 cut(s) 169, 580, 637, 781
MboII GAAGA 4 cut(s) 44, 550, 684, 834
MlsI TGGCCA 1 cut(s) 339
MluCI AATT 8 cut(s) 89, 199, 345, 459, 645, 816, 848, 862
MluNI TGGCCA 1 cut(s) 339
MlyI GAGTC 2 cut(s) 355, 905
MnlI CCTC 5 cut(s) 189, 251, 254, 570, 736
Mox20I TGGCCA 1 cut(s) 339
Mph1103I ATGCAT 2 cut(s) 40, 420
MscI TGGCCA 1 cut(s) 339
MseI TTAA 5 cut(s) 141, 159, 324, 903, 975
Msp20I TGGCCA 1 cut(s) 339
MspCI CTTAAG 1 cut(s) 974
MspI CCGG 2 cut(s) 59, 737
MspR9I CCNGG 3 cut(s) 60, 789, 1001
Mva1269I GAATGC 1 cut(s) 915
MvaI CCWGG 2 cut(s) 789, 1001
MwoI GCNNNNNNNGC 1 cut(s) 832
NciI CCSGG 1 cut(s) 60
NdeII GATC 4 cut(s) 169, 580, 637, 781
NlaIII CATG 6 cut(s) 273, 422, 478, 629, 777, 953
NlaIV GGNNCC 2 cut(s) 6, 834
NsiI ATGCAT 2 cut(s) 40, 420
PaeR7I CTCGAG 1 cut(s) 180
PcsI WCGNNNNNNNCGW 2 cut(s) 271, 725
PctI GAATGC 1 cut(s) 915
PfeI GAWTC 4 cut(s) 47, 522, 674, 923
PfoI TCCNGGA 1 cut(s) 787
PkrI GCNGC 4 cut(s) 132, 280, 604, 753
PleI GAGTC 2 cut(s) 355, 904
PpsI GAGTC 2 cut(s) 355, 904
Psp6I CCWGG 2 cut(s) 787, 999
PspGI CCWGG 2 cut(s) 787, 999
PspN4I GGNNCC 2 cut(s) 6, 834
PspPI GGNCC 3 cut(s) 26, 777, 791
PstI CTGCAG 1 cut(s) 604
RsaI GTAC 4 cut(s) 224, 251, 469, 568
RsaNI GTAC 4 cut(s) 223, 250, 468, 567
SaqAI TTAA 5 cut(s) 141, 159, 324, 903, 975
SatI GCNGC 4 cut(s) 131, 279, 603, 752
Sau3AI GATC 4 cut(s) 169, 580, 637, 781
Sau96I GGNCC 3 cut(s) 26, 777, 791
ScaI AGTACT 1 cut(s) 568
SchI GAGTC 2 cut(s) 355, 905
ScrFI CCNGG 3 cut(s) 60, 789, 1001
SfaNI GCATC 1 cut(s) 25
SfcI CTRYAG 1 cut(s) 600
Sfr274I CTCGAG 1 cut(s) 180
SinI GGWCC 3 cut(s) 26, 777, 791
SlaI CTCGAG 1 cut(s) 180
SmlI CTYRAG 2 cut(s) 180, 974
SmoI CTYRAG 2 cut(s) 180, 974
Sse9I AATT 8 cut(s) 89, 199, 345, 459, 645, 816, 848, 862
SsiI CCGC 2 cut(s) 444, 824
SspMI CTAG 2 cut(s) 365, 678
StyD4I CCNGG 3 cut(s) 58, 787, 999
StyI CCWWGG 1 cut(s) 827
TaaI ACNGT 1 cut(s) 616
TaiI ACGT 3 cut(s) 722, 970, 1010
TaqI TCGA 5 cut(s) 67, 181, 274, 728, 926
TasI AATT 8 cut(s) 89, 199, 345, 459, 645, 816, 848, 862
TatI WGTACW 1 cut(s) 566
TfiI GAWTC 4 cut(s) 47, 522, 674, 923
Tru1I TTAA 5 cut(s) 141, 159, 324, 903, 975
Tru9I TTAA 5 cut(s) 141, 159, 324, 903, 975
TscAI CASTG 2 cut(s) 861, 921
TseI GCWGC 4 cut(s) 130, 278, 602, 751
TspDTI ATGAA 6 cut(s) 33, 102, 390, 435, 491, 978
TspGWI ACGGA 3 cut(s) 259, 419, 645
TspRI CASTG 2 cut(s) 861, 921
Vha464I CTTAAG 1 cut(s) 974
VpaK11BI GGWCC 3 cut(s) 26, 777, 791
XapI RAATTY 2 cut(s) 816, 862
XbaI TCTAGA 1 cut(s) 364
XhoI CTCGAG 1 cut(s) 180
XspI CTAG 2 cut(s) 365, 678
ZrmI AGTACT 1 cut(s) 568
Zsp2I ATGCAT 2 cut(s) 40, 420
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.