Rorug03G0191900
NAC Family

NAC domain-containing protein 67-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0002868
Physical Location & Seq
Forward (+)
16869457 .. 16870651
1195 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0191900.1

Sequence Viewer

Length: 765 bp
ATGACGAAGGAGTTCGCCGTACCACCGGTGGTGTTTCCCTCCGGCGGAAACCCCAGCGCCGGATCCAACAACATCCAGCAACGCCGCGTCGCGACGGCGCCGTTTCAACCGCCTCGATCCTCCACCTCAAGCATCCCCTTCATGTCCTTCGACATTGGCTCCGCCGCCGCCTCCTCCTCCTCCGGCCCCCTATTCGGCGGCCCCATCGGCTCCGGCGGTTCGGTTCCCGGTGGCACAAGCTTCGAGGACGAGGAGCCACTCCTCGACGAGCTCGGCATCCATCCAGATCAAATATGGAGAAAGACCAAGTCGATCCTCAATCCCTTCCGGGCCAACCCGGCCGTCCACAAGGACTCGGACCTTTCCGGCCCGATCCTCCTCTACATGTCCCTCTGCCTCTTTCAATTGCTCGCCGGGAAGATCCAATTCGGCGTGATTCTCGGCTGGATCGTCGTCTCCTCCATCTTCCTCTACGTCGTCTTCAACATGTTGGCCGGCCGGAATGGGAACCTCGACCTGCACAGGTGTACGAGCGTCGTCGGCTACTGTTTGTTACCGGTGGTGATTTTATCAGCTGCCTCGCTGTTCGTGCCGCAAGGCGGGACTTTCAGGTTCGCGGTGGCCGGGGTCTTTGTTTTGTGGGCCACTAGGGTTTGTACGGCCCTTATGGTTTCTCTTGCTGATGGAGGTGACGAGCATCGTGGGCTGATAGCGTATGCGTGCTTCTTGATTTACACTCTGTTTTCGCTTCTCGTTATATTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAM

Protein Analysis

254

Amino Acids

27.02

Weight (kDa)

6.88

Isoelectric Point (pI)

52.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Yip1 PF04893 105 - 252 3.4e-09 Yip1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 525
AccB1I GGYRCC 1 cut(s) 97
AccII CGCG 3 cut(s) 87, 92, 617
AclWI GGATC 7 cut(s) 57, 70, 111, 307, 367, 415, 455
AcoI YGGCCR 4 cut(s) 339, 492, 496, 621
AcyI GRCGYC 1 cut(s) 98
AfaI GTAC 3 cut(s) 21, 529, 658
AfiI CCNNNNNNNGG 4 cut(s) 44, 59, 194, 599
AflIII ACRYGT 2 cut(s) 384, 486
AgeI ACCGGT 2 cut(s) 25, 556
AgsI TTSAA 3 cut(s) 107, 404, 484
AluBI AGCT 3 cut(s) 240, 271, 575
AluI AGCT 3 cut(s) 240, 271, 575
Alw21I GWGCWC 1 cut(s) 273
Alw26I GTCTC 1 cut(s) 460
AlwI GGATC 7 cut(s) 57, 70, 111, 307, 367, 415, 455
ApeKI GCWGC 1 cut(s) 575
AsiGI ACCGGT 2 cut(s) 25, 556
Asp700I GAANNNNTTC 1 cut(s) 11
AspLEI GCGC 2 cut(s) 59, 100
AspS9I GGNCC 7 cut(s) 185, 200, 330, 358, 368, 642, 661
AsuC2I CCSGG 5 cut(s) 228, 329, 338, 415, 625
AsuHPI GGTGA 2 cut(s) 574, 701
AvaII GGWCC 1 cut(s) 358
BamHI GGATCC 1 cut(s) 62
BanI GGYRCC 1 cut(s) 97
BanII GRGCYC 1 cut(s) 273
BarI GAAGNNNNNNTAC 2 cut(s) 707, 739
BbsI GAAGAC 1 cut(s) 472
Bbv12I GWGCWC 1 cut(s) 273
BbvI GCAGC 1 cut(s) 562
BccI CCATC 4 cut(s) 212, 288, 470, 677
BceAI ACGGC 5 cut(s) 2, 85, 111, 326, 675
BcgI CGANNNNNNTGC 2 cut(s) 223, 257
BcnI CCSGG 5 cut(s) 228, 329, 338, 415, 625
BcoDI GTCTC 1 cut(s) 460
BfaI CTAG 1 cut(s) 648
BfoI RGCGCY 2 cut(s) 60, 101
BfuAI ACCTGC 1 cut(s) 525
BglI GCCNNNNNGGC 2 cut(s) 207, 338
BisI GCNGC 6 cut(s) 85, 165, 168, 199, 576, 593
BlsI GCNGC 6 cut(s) 86, 166, 169, 200, 577, 594
Bme1390I CCNGG 5 cut(s) 228, 329, 338, 415, 625
Bme18I GGWCC 1 cut(s) 358
BmgT120I GGNCC 7 cut(s) 185, 200, 330, 358, 368, 642, 661
BmiI GGNNCC 9 cut(s) 64, 99, 160, 187, 202, 211, 225, 255, 509
BmrFI CCNGG 5 cut(s) 228, 329, 338, 415, 625
BmsI GCATC 3 cut(s) 141, 285, 706
BpiI GAAGAC 1 cut(s) 472
BpuEI CTTGAG 1 cut(s) 112
BpuMI CCSGG 5 cut(s) 228, 329, 338, 415, 625
BsaHI GRCGYC 1 cut(s) 98
BsaJI CCNNGG 1 cut(s) 624
BsaWI WCCGGW 2 cut(s) 25, 556
BsaXI ACNNNNNCTCC 2 cut(s) 143, 173
Bsc4I CCNNNNNNNGG 4 cut(s) 44, 59, 194, 599
Bse118I RCCGGY 3 cut(s) 25, 494, 556
BseDI CCNNGG 1 cut(s) 624
BseGI GGATG 4 cut(s) 72, 132, 276, 280
BseLI CCNNNNNNNGG 4 cut(s) 44, 59, 194, 599
BseRI GAGGAG 7 cut(s) 163, 166, 169, 251, 266, 368, 448
BseX3I CGGCCG 2 cut(s) 339, 496
BseXI GCAGC 1 cut(s) 562
BseYI CCCAGC 1 cut(s) 53
BsgI GTGCAG 1 cut(s) 503
Bsh1236I CGCG 3 cut(s) 87, 92, 617
Bsh1285I CGRYCG 2 cut(s) 342, 499
BshNI GGYRCC 1 cut(s) 97
BshTI ACCGGT 2 cut(s) 25, 556
BsiEI CGRYCG 2 cut(s) 342, 499
BsiHKAI GWGCWC 1 cut(s) 273
BslFI GGGAC 2 cut(s) 373, 616
BslI CCNNNNNNNGG 4 cut(s) 44, 59, 194, 599
BsmAI GTCTC 1 cut(s) 460
BsmBI CGTCTC 1 cut(s) 460
BsmFI GGGAC 2 cut(s) 373, 616
Bsp1286I GDGCHC 1 cut(s) 273
Bsp143I GATC 7 cut(s) 62, 116, 286, 312, 372, 420, 447
Bsp68I TCGCGA 1 cut(s) 92
BspFNI CGCG 3 cut(s) 87, 92, 617
BspLI GGNNCC 9 cut(s) 64, 99, 160, 187, 202, 211, 225, 255, 509
BspMI ACCTGC 1 cut(s) 525
BspPI GGATC 7 cut(s) 57, 70, 111, 307, 367, 415, 455
BspT107I GGYRCC 1 cut(s) 97
BsrFI RCCGGY 3 cut(s) 25, 494, 556
BssAI RCCGGY 3 cut(s) 25, 494, 556
BssECI CCNNGG 1 cut(s) 624
BssMI GATC 7 cut(s) 62, 116, 286, 312, 372, 420, 447
BssNI GRCGYC 1 cut(s) 98
Bst4CI ACNGT 1 cut(s) 548
BstACI GRCGYC 1 cut(s) 98
BstC8I GCNNGC 3 cut(s) 411, 496, 721
BstF5I GGATG 4 cut(s) 72, 132, 276, 280
BstFNI CGCG 3 cut(s) 87, 92, 617
BstH2I RGCGCY 2 cut(s) 60, 101
BstHHI GCGC 2 cut(s) 59, 100
BstKTI GATC 7 cut(s) 65, 119, 289, 315, 375, 423, 450
BstMAI GTCTC 1 cut(s) 460
BstMBI GATC 7 cut(s) 62, 116, 286, 312, 372, 420, 447
BstMCI CGRYCG 2 cut(s) 342, 499
BstMWI GCNNNNNNNGC 5 cut(s) 207, 338, 540, 589, 703
BstNSI RCATGY 2 cut(s) 388, 490
BstSCI CCNGG 5 cut(s) 226, 327, 336, 413, 623
BstUI CGCG 3 cut(s) 87, 92, 617
BstV1I GCAGC 1 cut(s) 562
BstV2I GAAGAC 1 cut(s) 472
BstX2I RGATCY 2 cut(s) 62, 420
BstYI RGATCY 2 cut(s) 62, 420
BstZI CGGCCG 2 cut(s) 339, 496
BtsCI GGATG 4 cut(s) 72, 132, 276, 280
BtuMI TCGCGA 1 cut(s) 92
BveI ACCTGC 1 cut(s) 525
Cac8I GCNNGC 3 cut(s) 411, 496, 721
CfoI GCGC 2 cut(s) 59, 100
Cfr10I RCCGGY 3 cut(s) 25, 494, 556
Cfr13I GGNCC 7 cut(s) 185, 200, 330, 358, 368, 642, 661
CseI GACGC 2 cut(s) 76, 523
Csp6I GTAC 3 cut(s) 20, 528, 657
CspAI ACCGGT 2 cut(s) 25, 556
CviAII CATG 3 cut(s) 142, 385, 487
CviQI GTAC 3 cut(s) 20, 528, 657
DinI GGCGCC 1 cut(s) 99
DpnI GATC 7 cut(s) 64, 118, 288, 314, 374, 422, 449
DpnII GATC 7 cut(s) 62, 116, 286, 312, 372, 420, 447
EaeI YGGCCR 4 cut(s) 339, 492, 496, 621
EagI CGGCCG 2 cut(s) 339, 496
EciI GGCGGA 2 cut(s) 60, 151
Ecl136II GAGCTC 1 cut(s) 271
EclXI CGGCCG 2 cut(s) 339, 496
Eco24I GRGCYC 1 cut(s) 273
Eco47I GGWCC 1 cut(s) 358
Eco52I CGGCCG 2 cut(s) 339, 496
Eco53kI GAGCTC 1 cut(s) 271
EcoICRI GAGCTC 1 cut(s) 271
EcoT38I GRGCYC 1 cut(s) 273
EgeI GGCGCC 1 cut(s) 99
EheI GGCGCC 1 cut(s) 99
Esp3I CGTCTC 1 cut(s) 460
FaeI CATG 3 cut(s) 145, 388, 490
FaiI YATR 7 cut(s) 143, 295, 386, 488, 668, 717, 758
FaqI GGGAC 2 cut(s) 373, 616
FatI CATG 3 cut(s) 141, 384, 486
FauI CCCGC 1 cut(s) 593
Fnu4HI GCNGC 6 cut(s) 85, 165, 168, 199, 576, 593
FokI GGATG 4 cut(s) 59, 119, 263, 267
FriOI GRGCYC 1 cut(s) 273
FseI GGCCGGCC 1 cut(s) 498
Fsp4HI GCNGC 6 cut(s) 85, 165, 168, 199, 576, 593
FspBI CTAG 1 cut(s) 648
GlaI GCGC 2 cut(s) 58, 99
GluI GCNGC 6 cut(s) 85, 165, 168, 199, 576, 593
GsaI CCCAGC 1 cut(s) 57
HaeII RGCGCY 2 cut(s) 60, 101
HgaI GACGC 2 cut(s) 76, 523
HhaI GCGC 2 cut(s) 59, 100
Hin1I GRCGYC 1 cut(s) 98
Hin1II CATG 3 cut(s) 145, 388, 490
Hin6I GCGC 2 cut(s) 57, 98
HinP1I GCGC 2 cut(s) 57, 98
HindIII AAGCTT 1 cut(s) 238
HinfI GANTC 2 cut(s) 353, 436
HphI GGTGA 2 cut(s) 574, 701
Hpy166II GTNNAC 2 cut(s) 346, 528
Hpy188I TCNGA 1 cut(s) 358
Hpy188III TCNNGA 3 cut(s) 91, 284, 727
Hpy8I GTNNAC 2 cut(s) 346, 528
Hpy99I CGWCG 7 cut(s) 92, 97, 269, 455, 479, 539, 542
HpyAV CCTTC 3 cut(s) 148, 157, 334
HpyCH4III ACNGT 1 cut(s) 548
HpyCH4IV ACGT 1 cut(s) 474
HpyCH4V TGCA 1 cut(s) 520
HpyF10VI GCNNNNNNNGC 5 cut(s) 207, 338, 540, 589, 703
HpySE526I ACGT 1 cut(s) 474
Hsp92I GRCGYC 1 cut(s) 98
Hsp92II CATG 3 cut(s) 145, 388, 490
HspAI GCGC 2 cut(s) 57, 98
KasI GGCGCC 1 cut(s) 97
KroI GCCGGC 1 cut(s) 494
KroNI GCCGGC 1 cut(s) 496
Kzo9I GATC 7 cut(s) 62, 116, 286, 312, 372, 420, 447
LmnI GCTCC 3 cut(s) 164, 215, 253
Lsp1109I GCAGC 1 cut(s) 562
LweI GCATC 3 cut(s) 141, 285, 706
MaeI CTAG 1 cut(s) 648
MaeII ACGT 1 cut(s) 474
MaeIII GTNAC 2 cut(s) 552, 689
MalI GATC 7 cut(s) 64, 118, 288, 314, 374, 422, 449
MboI GATC 7 cut(s) 62, 116, 286, 312, 372, 420, 447
MboII GAAGA 3 cut(s) 430, 457, 472
MfeI CAATTG 1 cut(s) 404
MflI RGATCY 2 cut(s) 62, 420
MhlI GDGCHC 1 cut(s) 273
MluCI AATT 2 cut(s) 404, 425
Mly113I GGCGCC 1 cut(s) 98
MlyI GAGTC 1 cut(s) 347
MmeI TCCRAC 1 cut(s) 90
MroNI GCCGGC 1 cut(s) 494
MroXI GAANNNNTTC 1 cut(s) 11
MspA1I CMGCKG 1 cut(s) 575
MspR9I CCNGG 5 cut(s) 228, 329, 338, 415, 625
MunI CAATTG 1 cut(s) 404
MvnI CGCG 3 cut(s) 87, 92, 617
MwoI GCNNNNNNNGC 5 cut(s) 207, 338, 540, 589, 703
NaeI GCCGGC 1 cut(s) 496
NarI GGCGCC 1 cut(s) 98
NciI CCSGG 5 cut(s) 228, 329, 338, 415, 625
NdeII GATC 7 cut(s) 62, 116, 286, 312, 372, 420, 447
NgoMIV GCCGGC 1 cut(s) 494
NlaIII CATG 3 cut(s) 145, 388, 490
NlaIV GGNNCC 9 cut(s) 64, 99, 160, 187, 202, 211, 225, 255, 509
NmeAIII GCCGAG 2 cut(s) 252, 420
NmuCI GTSAC 1 cut(s) 689
NruI TCGCGA 1 cut(s) 92
NspI RCATGY 2 cut(s) 388, 490
PciI ACATGT 2 cut(s) 384, 486
PcsI WCGNNNNNNNCGW 1 cut(s) 447
PdiI GCCGGC 1 cut(s) 496
PdmI GAANNNNTTC 1 cut(s) 11
PfeI GAWTC 1 cut(s) 436
PflFI GACNNNGTC 1 cut(s) 307
PinAI ACCGGT 2 cut(s) 25, 556
PkrI GCNGC 6 cut(s) 86, 166, 169, 200, 577, 594
PleI GAGTC 1 cut(s) 347
PluTI GGCGCC 1 cut(s) 101
PpsI GAGTC 1 cut(s) 347
PscI ACATGT 2 cut(s) 384, 486
Psp124BI GAGCTC 1 cut(s) 273
PspFI CCCAGC 1 cut(s) 53
PspN4I GGNNCC 9 cut(s) 64, 99, 160, 187, 202, 211, 225, 255, 509
PspPI GGNCC 7 cut(s) 185, 200, 330, 358, 368, 642, 661
PsuI RGATCY 2 cut(s) 62, 420
PsyI GACNNNGTC 1 cut(s) 307
PvuII CAGCTG 1 cut(s) 575
RigI GGCCGGCC 1 cut(s) 498
RruI TCGCGA 1 cut(s) 92
RsaI GTAC 3 cut(s) 21, 529, 658
RsaNI GTAC 3 cut(s) 20, 528, 657
SacI GAGCTC 1 cut(s) 273
SatI GCNGC 6 cut(s) 85, 165, 168, 199, 576, 593
Sau3AI GATC 7 cut(s) 62, 116, 286, 312, 372, 420, 447
Sau96I GGNCC 7 cut(s) 185, 200, 330, 358, 368, 642, 661
SchI GAGTC 1 cut(s) 347
ScrFI CCNGG 5 cut(s) 228, 329, 338, 415, 625
SduI GDGCHC 1 cut(s) 273
SfaNI GCATC 3 cut(s) 141, 285, 706
SfiI GGCCNNNNNGGCC 1 cut(s) 338
SfoI GGCGCC 1 cut(s) 99
SgrAI CRCCGGYG 1 cut(s) 25
SinI GGWCC 1 cut(s) 358
SmlI CTYRAG 1 cut(s) 127
SmoI CTYRAG 1 cut(s) 127
Sse9I AATT 2 cut(s) 404, 425
SspDI GGCGCC 1 cut(s) 97
SspMI CTAG 1 cut(s) 648
SstI GAGCTC 1 cut(s) 273
StyD4I CCNGG 5 cut(s) 226, 327, 336, 413, 623
TaaI ACNGT 1 cut(s) 548
TaiI ACGT 1 cut(s) 477
TaqI TCGA 6 cut(s) 115, 150, 243, 264, 311, 513
TasI AATT 2 cut(s) 404, 425
TauI GCSGC 5 cut(s) 87, 167, 170, 201, 595
TfiI GAWTC 1 cut(s) 436
TseFI GTSAC 1 cut(s) 689
TseI GCWGC 1 cut(s) 575
Tsp45I GTSAC 1 cut(s) 689
TspDTI ATGAA 1 cut(s) 130
Tth111I GACNNNGTC 1 cut(s) 307
VpaK11BI GGWCC 1 cut(s) 358
XceI RCATGY 2 cut(s) 388, 490
XcmI CCANNNNNNNNNTGG 1 cut(s) 291
XmnI GAANNNNTTC 1 cut(s) 11
XspI CTAG 1 cut(s) 648
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.