RLG00000023394
NAC Family

NAC domain-containing protein 67-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0002868
Physical Location & Seq
Reverse (-)
23033433 .. 23034835
1403 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023394

Sequence Viewer

Length: 747 bp
ATGTCGAACAACATGGTTCCAGTAGTCTTGAATGAGACTGAAGATTACTTTAATTCGATGCCACCCGGTTTTCGATTTGTTCCGACCGATGAAGTCTTGATTCAATGTTACTTGAGGAAGAAGTTATTGAACGAACCATTGCCCATTAACAGGATTCGTCATGTTAATATTTACCAATACCATCCTCGAGACCTTACCGCTACGTACGACCTCCTTAGAGAAAGTGAATGGTACTATTTCACTCCTATTACCCGAAAGTATGTGAACAGTTCTCGACCAAATCGAGTTGCACCGAATGGTTTTTGGAAGCCAAGAATGAGCAGCCCCATCAAAGATAGGAATAATGAAGTAGTTGGATACAGGACGAGTCTGGAATTTTATGAAGGGAAATATCCAAACGGAAATAAGACAGGGTGGAAAATGAATGAATATACAATCAATGAAGCAATTATTCCTCCAAATATTAATTCAAATGGCAAAACTCGCATCAAGTTGGATGACTGTGTTTTGTGCAAGATCTTTACGCTTAAGGTGGCCAGGAAGCTTCAGAATGAGATACAATATCAGTCGGAGAATCCTATAGGTCGTAACAATCAACATCAATTGTTGGTCCAAGTAACACTACTACAACTAGTGATCAGTTACCTGCGCATGACGCCATGGAACATGAGCCGCATAGTAACCATGAAGTGCAACAAGATGGTTCTTCTGATTCTAAGCACGAAGAAGAAGATGACGGTTGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAM

Protein Analysis

249

Amino Acids

29.17

Weight (kDa)

9.69

Isoelectric Point (pI)

43.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 21 - 145 1.8e-34 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 650
Acc36I ACCTGC 1 cut(s) 654
AciI CCGC 2 cut(s) 198, 673
AcoI YGGCCR 1 cut(s) 534
AcsI RAATTY 1 cut(s) 374
AcuI CTGAAG 2 cut(s) 60, 530
AcyI GRCGYC 1 cut(s) 656
AfaI GTAC 2 cut(s) 206, 233
AfiI CCNNNNNNNGG 1 cut(s) 150
AflII CTTAAG 1 cut(s) 527
AgsI TTSAA 4 cut(s) 31, 104, 130, 471
AhlI ACTAGT 1 cut(s) 631
AjnI CCWGG 1 cut(s) 536
AjuI GAANNNNNNNTTGG 2 cut(s) 451, 483
AluBI AGCT 1 cut(s) 544
AluI AGCT 1 cut(s) 544
Alw26I GTCTC 2 cut(s) 29, 183
Ama87I CYCGRG 1 cut(s) 186
AoxI GGCC 1 cut(s) 534
ApeKI GCWGC 1 cut(s) 321
ApoI RAATTY 1 cut(s) 374
AseI ATTAAT 1 cut(s) 465
AspLEI GCGC 1 cut(s) 651
AspS9I GGNCC 1 cut(s) 610
AsuC2I CCSGG 1 cut(s) 66
AvaI CYCGRG 1 cut(s) 186
AvaII GGWCC 1 cut(s) 610
BalI TGGCCA 1 cut(s) 536
BbvI GCAGC 1 cut(s) 333
BccI CCATC 3 cut(s) 189, 335, 694
BciT130I CCWGG 1 cut(s) 538
BciVI GTATCC 1 cut(s) 350
BclI TGATCA 1 cut(s) 636
BcnI CCSGG 1 cut(s) 66
BcoDI GTCTC 2 cut(s) 29, 183
BcuI ACTAGT 1 cut(s) 631
BfaI CTAG 1 cut(s) 632
BfmI CTRYAG 1 cut(s) 579
BfrI CTTAAG 1 cut(s) 527
BfuAI ACCTGC 1 cut(s) 654
BfuI GTATCC 1 cut(s) 350
BglII AGATCT 1 cut(s) 516
BisI GCNGC 2 cut(s) 322, 673
BlsI GCNGC 2 cut(s) 323, 674
Bme1390I CCNGG 2 cut(s) 66, 538
Bme18I GGWCC 1 cut(s) 610
BmeT110I CYCGRG 1 cut(s) 186
BmgT120I GGNCC 1 cut(s) 610
BmiI GGNNCC 1 cut(s) 18
BmrFI CCNGG 2 cut(s) 66, 538
BmsI GCATC 2 cut(s) 48, 495
BpuEI CTTGAG 1 cut(s) 133
BpuMI CCSGG 1 cut(s) 66
BsaAI YACGTR 1 cut(s) 204
BsaHI GRCGYC 1 cut(s) 656
BsaI GGTCTC 1 cut(s) 183
BsaJI CCNNGG 1 cut(s) 659
Bsc4I CCNNNNNNNGG 1 cut(s) 150
Bse1I ACTGG 1 cut(s) 20
Bse3DI GCAATG 1 cut(s) 137
BseBI CCWGG 1 cut(s) 538
BseDI CCNNGG 1 cut(s) 659
BseGI GGATG 2 cut(s) 181, 502
BseLI CCNNNNNNNGG 1 cut(s) 150
BseMI GCAATG 1 cut(s) 137
BseNI ACTGG 1 cut(s) 20
BseXI GCAGC 1 cut(s) 333
Bsh1285I CGRYCG 1 cut(s) 87
BshFI GGCC 1 cut(s) 536
BsiEI CGRYCG 1 cut(s) 87
BsiHKCI CYCGRG 1 cut(s) 186
BsiSI CCGG 1 cut(s) 66
BsiWI CGTACG 1 cut(s) 204
BslI CCNNNNNNNGG 1 cut(s) 150
BsmAI GTCTC 2 cut(s) 29, 183
BsnI GGCC 1 cut(s) 536
Bso31I GGTCTC 1 cut(s) 183
BsoBI CYCGRG 1 cut(s) 186
Bsp143I GATC 2 cut(s) 516, 636
Bsp19I CCATGG 1 cut(s) 659
BspACI CCGC 2 cut(s) 198, 673
BspANI GGCC 1 cut(s) 536
BspLI GGNNCC 1 cut(s) 18
BspMI ACCTGC 1 cut(s) 654
BspTI CTTAAG 1 cut(s) 527
BspTNI GGTCTC 1 cut(s) 183
BsrDI GCAATG 1 cut(s) 137
BsrI ACTGG 1 cut(s) 20
BssECI CCNNGG 1 cut(s) 659
BssMI GATC 2 cut(s) 516, 636
BssNI GRCGYC 1 cut(s) 656
BssT1I CCWWGG 1 cut(s) 659
Bst2UI CCWGG 1 cut(s) 538
Bst4CI ACNGT 3 cut(s) 269, 503, 739
BstACI GRCGYC 1 cut(s) 656
BstAFI CTTAAG 1 cut(s) 527
BstBAI YACGTR 1 cut(s) 204
BstDEI CTNAG 2 cut(s) 215, 716
BstDSI CCRYGG 1 cut(s) 659
BstF5I GGATG 2 cut(s) 181, 502
BstHHI GCGC 1 cut(s) 651
BstKTI GATC 2 cut(s) 519, 639
BstMAI GTCTC 2 cut(s) 29, 183
BstMBI GATC 2 cut(s) 516, 636
BstMCI CGRYCG 1 cut(s) 87
BstMWI GCNNNNNNNGC 2 cut(s) 483, 655
BstNI CCWGG 1 cut(s) 538
BstSCI CCNGG 2 cut(s) 64, 536
BstSFI CTRYAG 1 cut(s) 579
BstSNI TACGTA 1 cut(s) 204
BstV1I GCAGC 1 cut(s) 333
BstX2I RGATCY 1 cut(s) 516
BstYI RGATCY 1 cut(s) 516
BsuI GTATCC 1 cut(s) 350
BsuRI GGCC 1 cut(s) 536
BtgI CCRYGG 1 cut(s) 659
BtsCI GGATG 2 cut(s) 181, 502
BveI ACCTGC 1 cut(s) 654
CfoI GCGC 1 cut(s) 651
Cfr13I GGNCC 1 cut(s) 610
CseI GACGC 1 cut(s) 664
Csp6I GTAC 2 cut(s) 205, 232
CviAII CATG 6 cut(s) 13, 161, 652, 660, 667, 685
CviJI RGCY 5 cut(s) 310, 324, 536, 544, 672
CviKI_1 RGCY 5 cut(s) 310, 324, 536, 544, 672
CviQI GTAC 2 cut(s) 205, 232
DdeI CTNAG 2 cut(s) 215, 716
DpnI GATC 2 cut(s) 518, 638
DpnII GATC 2 cut(s) 516, 636
EaeI YGGCCR 1 cut(s) 534
Eco105I TACGTA 1 cut(s) 204
Eco130I CCWWGG 1 cut(s) 659
Eco31I GGTCTC 1 cut(s) 183
Eco47I GGWCC 1 cut(s) 610
Eco57I CTGAAG 2 cut(s) 60, 530
Eco88I CYCGRG 1 cut(s) 186
EcoRII CCWGG 1 cut(s) 536
EcoT14I CCWWGG 1 cut(s) 659
ErhI CCWWGG 1 cut(s) 659
FaeI CATG 6 cut(s) 16, 164, 655, 663, 670, 688
FatI CATG 6 cut(s) 12, 160, 651, 659, 666, 684
FbaI TGATCA 1 cut(s) 636
Fnu4HI GCNGC 2 cut(s) 322, 673
FokI GGATG 2 cut(s) 168, 509
Fsp4HI GCNGC 2 cut(s) 322, 673
FspBI CTAG 1 cut(s) 632
FspI TGCGCA 1 cut(s) 650
GlaI GCGC 1 cut(s) 650
GluI GCNGC 2 cut(s) 322, 673
HaeIII GGCC 1 cut(s) 536
HapII CCGG 1 cut(s) 66
HgaI GACGC 1 cut(s) 664
HhaI GCGC 1 cut(s) 651
Hin1I GRCGYC 1 cut(s) 656
Hin1II CATG 6 cut(s) 16, 164, 655, 663, 670, 688
Hin6I GCGC 1 cut(s) 649
HinP1I GCGC 1 cut(s) 649
HindIII AAGCTT 1 cut(s) 542
HinfI GANTC 5 cut(s) 100, 154, 367, 574, 712
HpaII CCGG 1 cut(s) 66
Hpy166II GTNNAC 1 cut(s) 265
Hpy188I TCNGA 4 cut(s) 84, 549, 571, 711
Hpy188III TCNNGA 5 cut(s) 28, 97, 188, 273, 371
Hpy8I GTNNAC 1 cut(s) 265
HpyAV CCTTC 1 cut(s) 377
HpyCH4III ACNGT 3 cut(s) 269, 503, 739
HpyCH4IV ACGT 1 cut(s) 203
HpyCH4V TGCA 3 cut(s) 290, 513, 693
HpyF10VI GCNNNNNNNGC 2 cut(s) 483, 655
HpyF3I CTNAG 2 cut(s) 215, 716
HpySE526I ACGT 1 cut(s) 203
Hsp92I GRCGYC 1 cut(s) 656
Hsp92II CATG 6 cut(s) 16, 164, 655, 663, 670, 688
HspAI GCGC 1 cut(s) 649
Ksp22I TGATCA 1 cut(s) 636
Kzo9I GATC 2 cut(s) 516, 636
LpnPI CCDG 9 cut(s) 33, 79, 136, 346, 356, 396, 523, 550, 659
Lsp1109I GCAGC 1 cut(s) 333
LweI GCATC 2 cut(s) 48, 495
MaeI CTAG 1 cut(s) 632
MaeII ACGT 1 cut(s) 203
MaeIII GTNAC 5 cut(s) 107, 587, 616, 641, 679
MalI GATC 2 cut(s) 518, 638
MboI GATC 2 cut(s) 516, 636
MboII GAAGA 6 cut(s) 53, 130, 698, 736, 739, 742
MfeI CAATTG 1 cut(s) 602
MflI RGATCY 1 cut(s) 516
MlsI TGGCCA 1 cut(s) 536
MluCI AATT 5 cut(s) 52, 374, 447, 466, 602
MluNI TGGCCA 1 cut(s) 536
MlyI GAGTC 1 cut(s) 376
MmeI TCCRAC 4 cut(s) 107, 334, 474, 549
MnlI CCTC 4 cut(s) 108, 195, 221, 465
Mox20I TGGCCA 1 cut(s) 536
MscI TGGCCA 1 cut(s) 536
MseI TTAA 5 cut(s) 51, 147, 165, 465, 528
Msp20I TGGCCA 1 cut(s) 536
MspCI CTTAAG 1 cut(s) 527
MspI CCGG 1 cut(s) 66
MspR9I CCNGG 2 cut(s) 66, 538
MunI CAATTG 1 cut(s) 602
MvaI CCWGG 1 cut(s) 538
MwoI GCNNNNNNNGC 2 cut(s) 483, 655
NciI CCSGG 1 cut(s) 66
NcoI CCATGG 1 cut(s) 659
NdeII GATC 2 cut(s) 516, 636
NlaIII CATG 6 cut(s) 16, 164, 655, 663, 670, 688
NlaIV GGNNCC 1 cut(s) 18
NsbI TGCGCA 1 cut(s) 650
PaeR7I CTCGAG 1 cut(s) 186
PcsI WCGNNNNNNNCGW 1 cut(s) 280
PfeI GAWTC 4 cut(s) 100, 154, 574, 712
Pfl23II CGTACG 1 cut(s) 204
PkrI GCNGC 2 cut(s) 323, 674
PleI GAGTC 1 cut(s) 375
PpsI GAGTC 1 cut(s) 375
Ppu21I YACGTR 1 cut(s) 204
PshBI ATTAAT 1 cut(s) 465
Psp6I CCWGG 1 cut(s) 536
PspGI CCWGG 1 cut(s) 536
PspLI CGTACG 1 cut(s) 204
PspN4I GGNNCC 1 cut(s) 18
PspPI GGNCC 1 cut(s) 610
PsuI RGATCY 1 cut(s) 516
RsaI GTAC 2 cut(s) 206, 233
RsaNI GTAC 2 cut(s) 205, 232
SaqAI TTAA 5 cut(s) 51, 147, 165, 465, 528
SatI GCNGC 2 cut(s) 322, 673
Sau3AI GATC 2 cut(s) 516, 636
Sau96I GGNCC 1 cut(s) 610
SchI GAGTC 1 cut(s) 376
ScrFI CCNGG 2 cut(s) 66, 538
SetI ASST 7 cut(s) 195, 206, 213, 534, 546, 586, 648
SfaNI GCATC 2 cut(s) 48, 495
SfcI CTRYAG 1 cut(s) 579
Sfr274I CTCGAG 1 cut(s) 186
SinI GGWCC 1 cut(s) 610
SlaI CTCGAG 1 cut(s) 186
SmlI CTYRAG 3 cut(s) 112, 186, 527
SmoI CTYRAG 3 cut(s) 112, 186, 527
SnaBI TACGTA 1 cut(s) 204
SpeI ACTAGT 1 cut(s) 631
Sse9I AATT 5 cut(s) 52, 374, 447, 466, 602
SsiI CCGC 2 cut(s) 198, 673
SspI AATATT 2 cut(s) 169, 463
SspMI CTAG 1 cut(s) 632
StyD4I CCNGG 2 cut(s) 64, 536
StyI CCWWGG 1 cut(s) 659
TaaI ACNGT 3 cut(s) 269, 503, 739
TaiI ACGT 1 cut(s) 206
TaqI TCGA 6 cut(s) 5, 56, 73, 187, 274, 283
TaqII GACCGA 1 cut(s) 101
TasI AATT 5 cut(s) 52, 374, 447, 466, 602
TauI GCSGC 1 cut(s) 675
TfiI GAWTC 4 cut(s) 100, 154, 574, 712
Tru1I TTAA 5 cut(s) 51, 147, 165, 465, 528
Tru9I TTAA 5 cut(s) 51, 147, 165, 465, 528
TseI GCWGC 1 cut(s) 321
TspDTI ATGAA 7 cut(s) 105, 360, 396, 437, 441, 456, 701
TspGWI ACGGA 1 cut(s) 414
Vha464I CTTAAG 1 cut(s) 527
VpaK11BI GGWCC 1 cut(s) 610
VspI ATTAAT 1 cut(s) 465
XapI RAATTY 1 cut(s) 374
XhoI CTCGAG 1 cut(s) 186
XspI CTAG 1 cut(s) 632
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.