Rroxscaffold_6G00400420
NAC Family

NAC domain-containing protein 67-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0002868
Physical Location & Seq
Reverse (-)
22513478 .. 22515448
1971 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00400420.1

Sequence Viewer

Length: 1482 bp
ATGGTTCCAAACAATCAGCAAAGTGATACGATGTCTGATGGAGAGATTGATGATTATTTTAGATCTTTTCCACCAGGTTATCAATTTGTCCCCACTGATGCAGAGTTGATTATATATTATTTGAAGAGAAAGATCTTGAATCAACAACTACCCATGAACCGGATTCGTGATGAGAATATTTATCACTACCATCCTCAAACTCTTGCTGAATTGTATCCGAGAATTAGAGAAATTGAATGGTACTTTTTCTCTTCTAGAGACCGAAGGTACCCCAATGGATCTCGGCCTAATCGATCAGCACAAGGTGGATACTGGAAGGCAACGAAAAGAGATGAGATCATCACAGCTCGAGGTGGTGAAGAAATTGGATTCAAAATGCATCTGGAATTTTATGAAGGAACACATGCACAAGGTAGAAAGACAAACTGGAAAATGCATGAATATAGGATCAAAGAAGAGAATGAAGAGAATGATATTGGTCCTCCTCAAAATACGAATAAGGGTGAAGGTATCAGCATGAGGTTGGATGATTGGGTTTTGTGTAAGATTTACATAAAGAAGAATTTCAAGGCTGATACAGACAGCAACAGTTCAAAGACATCAGACAATTCTGATCAAGTAGTACTCCCAGTAACAAATGTTACAACATCTCGTGATTGTCAAAATAAACTAGCTATTCAGCATGAAGTTCAAGATCAATCTCTCAATGCCTCACAGTTCGACCACAATGGCTTCTCAACTTTGACGAACTATCTTGATGTAGAATCCAGCAGCAGCAACTTCTGGAATCAAAACTATACAGATGATTACGACATGTACTTGATGCAGATGAGTCGAGATCCATCATCGTCACGATTCTTTTCTTCTCCGTTTCCCTTGCAATCTTCAACAGACCAGATTTGCGCAGGATTGGAGCCTGTAGATGATAAGCTATCAGAGGTCGATCCAAGTTCTATGTTACCTGCGCATGTCAGAGAATGTGATGAGAATATGTCAGAGAATAAGAATCAAAACTATACAGATGATTTCGACACGTACTTGATGCAGATGAGTCGAGATCCATCATCGTCACGATTCTTGTCTTCTCCGTTTCCCTTGCAATCTTCAACAGATCAGATTTGCGCAGGATTGGAGCCTGTAGATGATAAGATATCAGAGGTCGATCCAAGTTCTATGTTACCTGCGCATGCCAGAGAATTTGATGAGAATATGTCAGAGAATAAGAATCAAAACTATACAGATGATTACGACACGTACTTGATGCAGATGAGTCGAGATCCATCGTCGTCACGATTATTTTCTTCTCCGTTTCCCTTGCAATCTTCAACAGACCAGATTTGCGCAGGATTGGAGCCTGTAGATGATAAGATATCAGAGGTCGATCCAAGTTCTATTGTGTTACCTGCGCATGTCAGAGAATATGATAATCGTGCTTGTAAAGCCGAGCCTATTGATGAAGGTGATGATCATGTGTCTAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAM

Protein Analysis

493

Amino Acids

56.77

Weight (kDa)

4.64

Isoelectric Point (pI)

62.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 24 - 150 2.7e-38 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 6 cut(s) 904, 966, 1123, 1185, 1342, 1407
Acc36I ACCTGC 3 cut(s) 970, 1189, 1411
Acc65I GGTACC 1 cut(s) 267
AccB1I GGYRCC 1 cut(s) 267
AclWI GGATC 8 cut(s) 286, 455, 833, 938, 1052, 1157, 1271, 1376
AcsI RAATTY 3 cut(s) 386, 562, 1196
AdeI CACNNNGTG 1 cut(s) 305
AfaI GTAC 6 cut(s) 242, 269, 624, 818, 1037, 1256
AfiI CCNNNNNNNGG 1 cut(s) 159
AflIII ACRYGT 3 cut(s) 813, 1032, 1251
AjnI CCWGG 1 cut(s) 73
AluBI AGCT 3 cut(s) 347, 674, 931
AluI AGCT 3 cut(s) 347, 674, 931
Alw26I GTCTC 1 cut(s) 252
AlwI GGATC 8 cut(s) 286, 455, 833, 938, 1052, 1157, 1271, 1376
Ama87I CYCGRG 1 cut(s) 348
AoxI GGCC 1 cut(s) 284
ApeKI GCWGC 2 cut(s) 771, 774
ApoI RAATTY 3 cut(s) 386, 562, 1196
Asp700I GAANNNNTTC 1 cut(s) 563
Asp718I GGTACC 1 cut(s) 267
AspLEI GCGC 6 cut(s) 905, 967, 1124, 1186, 1343, 1408
AspS9I GGNCC 1 cut(s) 479
AsuHPI GGTGA 3 cut(s) 368, 515, 1472
AvaI CYCGRG 1 cut(s) 348
AvaII GGWCC 1 cut(s) 479
BanI GGYRCC 1 cut(s) 267
BauI CACGAG 1 cut(s) 651
BbsI GAAGAC 1 cut(s) 1074
BbvI GCAGC 2 cut(s) 783, 786
BccI CCATC 5 cut(s) 32, 198, 850, 1069, 1288
BcgI CGANNNNNNTGC 6 cut(s) 815, 849, 1034, 1068, 1253, 1287
BciT130I CCWGG 1 cut(s) 75
BciVI GTATCC 2 cut(s) 225, 302
BclI TGATCA 2 cut(s) 613, 1465
BcoDI GTCTC 1 cut(s) 252
BfaI CTAG 2 cut(s) 255, 671
BfmI CTRYAG 3 cut(s) 918, 1137, 1356
BfuAI ACCTGC 3 cut(s) 970, 1189, 1411
BfuI GTATCC 2 cut(s) 225, 302
BglII AGATCT 2 cut(s) 62, 132
BisI GCNGC 2 cut(s) 772, 775
BlsI GCNGC 2 cut(s) 773, 776
BmcAI AGTACT 1 cut(s) 624
Bme1390I CCNGG 1 cut(s) 75
Bme18I GGWCC 1 cut(s) 479
BmeT110I CYCGRG 1 cut(s) 348
BmgT120I GGNCC 1 cut(s) 479
BmiI GGNNCC 5 cut(s) 6, 269, 915, 1134, 1353
BmrFI CCNGG 1 cut(s) 75
BmrI ACTGGG 1 cut(s) 623
BmsI GCATC 5 cut(s) 88, 388, 813, 1032, 1251
BmuI ACTGGG 1 cut(s) 623
BpiI GAAGAC 1 cut(s) 1074
Bsa29I ATCGAT 1 cut(s) 292
BsaAI YACGTR 2 cut(s) 1035, 1254
BsaI GGTCTC 1 cut(s) 252
BsaWI WCCGGW 1 cut(s) 159
Bsc4I CCNNNNNNNGG 1 cut(s) 159
Bse1I ACTGG 3 cut(s) 317, 431, 629
BseBI CCWGG 1 cut(s) 75
BseCI ATCGAT 1 cut(s) 292
BseGI GGATG 2 cut(s) 190, 532
BseLI CCNNNNNNNGG 1 cut(s) 159
BseNI ACTGG 3 cut(s) 317, 431, 629
BseRI GAGGAG 1 cut(s) 474
BseXI GCAGC 2 cut(s) 783, 786
BshFI GGCC 1 cut(s) 286
BshNI GGYRCC 1 cut(s) 267
BshVI ATCGAT 1 cut(s) 292
BsiHKCI CYCGRG 1 cut(s) 348
BsiSI CCGG 1 cut(s) 160
BslFI GGGAC 1 cut(s) 74
BslI CCNNNNNNNGG 1 cut(s) 159
BsmAI GTCTC 1 cut(s) 252
BsmFI GGGAC 1 cut(s) 74
BsnI GGCC 1 cut(s) 286
Bso31I GGTCTC 1 cut(s) 252
BsoBI CYCGRG 1 cut(s) 348
BspANI GGCC 1 cut(s) 286
BspDI ATCGAT 1 cut(s) 292
BspLI GGNNCC 5 cut(s) 6, 269, 915, 1134, 1353
BspMI ACCTGC 3 cut(s) 970, 1189, 1411
BspPI GGATC 8 cut(s) 286, 455, 833, 938, 1052, 1157, 1271, 1376
BspT107I GGYRCC 1 cut(s) 267
BspTNI GGTCTC 1 cut(s) 252
BsrI ACTGG 3 cut(s) 317, 431, 629
BssSI CACGAG 1 cut(s) 651
Bst2BI CACGAG 1 cut(s) 651
Bst2UI CCWGG 1 cut(s) 75
Bst4CI ACNGT 2 cut(s) 590, 717
Bst6I CTCTTC 4 cut(s) 119, 256, 450, 459
BstBAI YACGTR 2 cut(s) 1035, 1254
BstC8I GCNNGC 1 cut(s) 1188
BstF5I GGATG 2 cut(s) 190, 532
BstHHI GCGC 6 cut(s) 905, 967, 1124, 1186, 1343, 1408
BstMAI GTCTC 1 cut(s) 252
BstMWI GCNNNNNNNGC 1 cut(s) 1439
BstNI CCWGG 1 cut(s) 75
BstNSI RCATGY 5 cut(s) 407, 817, 971, 1190, 1412
BstSCI CCNGG 1 cut(s) 73
BstSFI CTRYAG 3 cut(s) 918, 1137, 1356
BstV1I GCAGC 2 cut(s) 783, 786
BstV2I GAAGAC 1 cut(s) 1074
BstX2I RGATCY 6 cut(s) 62, 132, 278, 838, 1057, 1276
BstYI RGATCY 6 cut(s) 62, 132, 278, 838, 1057, 1276
Bsu15I ATCGAT 1 cut(s) 292
BsuI GTATCC 2 cut(s) 225, 302
BsuRI GGCC 1 cut(s) 286
BsuTUI ATCGAT 1 cut(s) 292
BtsCI GGATG 2 cut(s) 190, 532
BtsIMutI CAGTG 1 cut(s) 93
BveI ACCTGC 3 cut(s) 970, 1189, 1411
Cac8I GCNNGC 1 cut(s) 1188
CfoI GCGC 6 cut(s) 905, 967, 1124, 1186, 1343, 1408
Cfr13I GGNCC 1 cut(s) 479
ClaI ATCGAT 1 cut(s) 292
CsiI ACCWGGT 1 cut(s) 73
Csp6I GTAC 6 cut(s) 241, 268, 623, 817, 1036, 1255
CviQI GTAC 6 cut(s) 241, 268, 623, 817, 1036, 1255
DraIII CACNNNGTG 1 cut(s) 305
Eam1104I CTCTTC 4 cut(s) 119, 256, 450, 459
EarI CTCTTC 4 cut(s) 119, 256, 450, 459
Eco31I GGTCTC 1 cut(s) 252
Eco32I GATATC 2 cut(s) 1152, 1371
Eco47I GGWCC 1 cut(s) 479
Eco88I CYCGRG 1 cut(s) 348
EcoRII CCWGG 1 cut(s) 73
EcoRV GATATC 2 cut(s) 1152, 1371
EcoT22I ATGCAT 2 cut(s) 381, 438
FaqI GGGAC 1 cut(s) 74
FbaI TGATCA 2 cut(s) 613, 1465
Fnu4HI GCNGC 2 cut(s) 772, 775
FokI GGATG 2 cut(s) 177, 539
Fsp4HI GCNGC 2 cut(s) 772, 775
FspBI CTAG 2 cut(s) 255, 671
FspI TGCGCA 6 cut(s) 904, 966, 1123, 1185, 1342, 1407
GlaI GCGC 6 cut(s) 904, 966, 1123, 1185, 1342, 1407
GluI GCNGC 2 cut(s) 772, 775
HaeIII GGCC 1 cut(s) 286
HapII CCGG 1 cut(s) 160
HhaI GCGC 6 cut(s) 905, 967, 1124, 1186, 1343, 1408
Hin6I GCGC 6 cut(s) 903, 965, 1122, 1184, 1341, 1406
HinP1I GCGC 6 cut(s) 903, 965, 1122, 1184, 1341, 1406
HpaII CCGG 1 cut(s) 160
HphI GGTGA 3 cut(s) 368, 515, 1472
Hpy99I CGWCG 1 cut(s) 1288
HpyAV CCTTC 5 cut(s) 258, 310, 389, 500, 1451
HpyCH4III ACNGT 2 cut(s) 590, 717
HpyCH4IV ACGT 2 cut(s) 1034, 1253
HpyF10VI GCNNNNNNNGC 1 cut(s) 1439
HpySE526I ACGT 2 cut(s) 1034, 1253
HspAI GCGC 6 cut(s) 903, 965, 1122, 1184, 1341, 1406
KpnI GGTACC 1 cut(s) 271
Ksp22I TGATCA 2 cut(s) 613, 1465
LmnI GCTCC 3 cut(s) 913, 1132, 1351
Lsp1109I GCAGC 2 cut(s) 783, 786
LweI GCATC 5 cut(s) 88, 388, 813, 1032, 1251
MabI ACCWGGT 1 cut(s) 73
MaeI CTAG 2 cut(s) 255, 671
MaeII ACGT 2 cut(s) 1034, 1253
MaeIII GTNAC 8 cut(s) 631, 640, 849, 957, 1068, 1176, 1287, 1398
MflI RGATCY 6 cut(s) 62, 132, 278, 838, 1057, 1276
MluCI AATT 9 cut(s) 83, 209, 222, 231, 363, 386, 562, 607, 1196
MlyI GAGTC 3 cut(s) 841, 1060, 1279
MmeI TCCRAC 1 cut(s) 504
MnlI CCTC 9 cut(s) 204, 344, 492, 495, 513, 721, 931, 1150, 1369
Mph1103I ATGCAT 2 cut(s) 381, 438
MroXI GAANNNNTTC 1 cut(s) 563
MspI CCGG 1 cut(s) 160
MspR9I CCNGG 1 cut(s) 75
MvaI CCWGG 1 cut(s) 75
MwoI GCNNNNNNNGC 1 cut(s) 1439
NlaIV GGNNCC 5 cut(s) 6, 269, 915, 1134, 1353
NmeAIII GCCGAG 2 cut(s) 262, 1468
NmuCI GTSAC 3 cut(s) 849, 1068, 1287
NsbI TGCGCA 6 cut(s) 904, 966, 1123, 1185, 1342, 1407
NsiI ATGCAT 2 cut(s) 381, 438
NspI RCATGY 5 cut(s) 407, 817, 971, 1190, 1412
PaeI GCATGC 1 cut(s) 1190
PaeR7I CTCGAG 1 cut(s) 348
PciI ACATGT 1 cut(s) 813
PcsI WCGNNNNNNNCGW 1 cut(s) 289
PdmI GAANNNNTTC 1 cut(s) 563
PfeI GAWTC 9 cut(s) 139, 163, 369, 764, 787, 855, 1006, 1074, 1225
PkrI GCNGC 2 cut(s) 773, 776
PleI GAGTC 3 cut(s) 840, 1059, 1278
PpsI GAGTC 3 cut(s) 840, 1059, 1278
Ppu21I YACGTR 2 cut(s) 1035, 1254
PscI ACATGT 1 cut(s) 813
Psp6I CCWGG 1 cut(s) 73
PspGI CCWGG 1 cut(s) 73
PspN4I GGNNCC 5 cut(s) 6, 269, 915, 1134, 1353
PspPI GGNCC 1 cut(s) 479
PspXI VCTCGAGB 1 cut(s) 348
PsuI RGATCY 6 cut(s) 62, 132, 278, 838, 1057, 1276
RsaI GTAC 6 cut(s) 242, 269, 624, 818, 1037, 1256
RsaNI GTAC 6 cut(s) 241, 268, 623, 817, 1036, 1255
SatI GCNGC 2 cut(s) 772, 775
Sau96I GGNCC 1 cut(s) 479
ScaI AGTACT 1 cut(s) 624
SchI GAGTC 3 cut(s) 841, 1060, 1279
ScrFI CCNGG 1 cut(s) 75
SexAI ACCWGGT 1 cut(s) 73
SfaNI GCATC 5 cut(s) 88, 388, 813, 1032, 1251
SfcI CTRYAG 3 cut(s) 918, 1137, 1356
Sfr274I CTCGAG 1 cut(s) 348
SinI GGWCC 1 cut(s) 479
SlaI CTCGAG 1 cut(s) 348
SmlI CTYRAG 1 cut(s) 348
SmoI CTYRAG 1 cut(s) 348
SphI GCATGC 1 cut(s) 1190
Sse9I AATT 9 cut(s) 83, 209, 222, 231, 363, 386, 562, 607, 1196
SspI AATATT 1 cut(s) 178
SspMI CTAG 2 cut(s) 255, 671
StyD4I CCNGG 1 cut(s) 73
TaaI ACNGT 2 cut(s) 590, 717
TaiI ACGT 2 cut(s) 1037, 1256
TaqII GACCGA 1 cut(s) 276
TasI AATT 9 cut(s) 83, 209, 222, 231, 363, 386, 562, 607, 1196
TatI WGTACW 2 cut(s) 622, 816
TfiI GAWTC 9 cut(s) 139, 163, 369, 764, 787, 855, 1006, 1074, 1225
TscAI CASTG 1 cut(s) 100
TseFI GTSAC 3 cut(s) 849, 1068, 1287
TseI GCWGC 2 cut(s) 771, 774
Tsp45I GTSAC 3 cut(s) 849, 1068, 1287
TspDTI ATGAA 6 cut(s) 170, 408, 453, 477, 699, 1470
TspGWI ACGGA 3 cut(s) 858, 1077, 1296
TspRI CASTG 1 cut(s) 100
VpaK11BI GGWCC 1 cut(s) 479
XapI RAATTY 3 cut(s) 386, 562, 1196
XbaI TCTAGA 1 cut(s) 254
XceI RCATGY 5 cut(s) 407, 817, 971, 1190, 1412
XhoI CTCGAG 1 cut(s) 348
XmnI GAANNNNTTC 1 cut(s) 563
XspI CTAG 2 cut(s) 255, 671
ZrmI AGTACT 1 cut(s) 624
Zsp2I ATGCAT 2 cut(s) 381, 438
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.