pycom12g06060
NAC Family

NAC domain-containing protein 67-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0002868
Physical Location & Seq
Forward (+)
6125867 .. 6127082
1216 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g06060.1

Sequence Viewer

Length: 477 bp
ATGGAAGAAAACAATCAGTTCAACAACAATATGGTTCCAGTTCTCAATGAAGTAGGTTCGGTTGATGCACACATTGATTCTCTTCCACCAGGTTATCGCTTCATCCTTACCGACGATGAGTTGATTTTACACTACTTAATGAGAAAGTTTATGAATGAGCAGCTTCCCATTAACAGGTTTCTTAATGTTAACATCTACCAGCACCATCCTCGAGAACTTACCGACGCTAATGGCCAAGTAATTGGGTCTAAAGGTACTCCAAATTATTATGAAGGGATACAACGAGAAGGGACAAGGACAGACTGGAAAATGCATGAATATAAGCTCACTAATGAGACTGATCCTCCAAAGAAAATAGATGGTACTGACATGAAGGTATGGAAGTGTGCACGTATGTGTGTGTCATCTGATAGAAGTATTTGCGTGGGTACAATTGTGGACTCTTTATATTGCGAACCACAATTCGGAATTTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAM

Protein Analysis

159

Amino Acids

18.3

Weight (kDa)

5.31

Isoelectric Point (pI)

28.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 29 - 76 1e-08 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 335
AcoI YGGCCR 1 cut(s) 232
AcsI RAATTY 1 cut(s) 468
AfaI GTAC 3 cut(s) 256, 364, 430
AfiI CCNNNNNNNGG 2 cut(s) 174, 464
AgsI TTSAA 1 cut(s) 22
AjnI CCWGG 1 cut(s) 88
AleI CACNNNNGTG 1 cut(s) 394
AluBI AGCT 2 cut(s) 163, 325
AluI AGCT 2 cut(s) 163, 325
Alw21I GWGCWC 1 cut(s) 391
Alw26I GTCTC 1 cut(s) 329
Alw44I GTGCAC 1 cut(s) 387
AlwI GGATC 1 cut(s) 335
Ama87I CYCGRG 1 cut(s) 210
AoxI GGCC 1 cut(s) 232
ApaLI GTGCAC 1 cut(s) 387
ApeKI GCWGC 1 cut(s) 160
ApoI RAATTY 1 cut(s) 468
AvaI CYCGRG 1 cut(s) 210
BaeGI GKGCMC 1 cut(s) 391
BaeI ACNNNNGTAYC 2 cut(s) 420, 453
BalI TGGCCA 1 cut(s) 234
Bbv12I GWGCWC 1 cut(s) 391
BbvI GCAGC 1 cut(s) 172
BccI CCATC 2 cut(s) 213, 353
BcgI CGANNNNNNTGC 2 cut(s) 191, 225
BciT130I CCWGG 1 cut(s) 90
BciVI GTATCC 1 cut(s) 270
BcoDI GTCTC 1 cut(s) 329
BfaI CTAG 1 cut(s) 475
BfuI GTATCC 1 cut(s) 270
BisI GCNGC 1 cut(s) 161
BlsI GCNGC 1 cut(s) 162
Bme1390I CCNGG 1 cut(s) 90
BmeT110I CYCGRG 1 cut(s) 210
BmiI GGNNCC 1 cut(s) 36
BmrFI CCNGG 1 cut(s) 90
BmsI GCATC 1 cut(s) 55
BsaAI YACGTR 1 cut(s) 392
BsaXI ACNNNNNCTCC 2 cut(s) 328, 358
Bsc4I CCNNNNNNNGG 2 cut(s) 174, 464
Bse1I ACTGG 2 cut(s) 38, 308
BseBI CCWGG 1 cut(s) 90
BseGI GGATG 2 cut(s) 102, 205
BseLI CCNNNNNNNGG 2 cut(s) 174, 464
BseNI ACTGG 2 cut(s) 38, 308
BseSI GKGCMC 1 cut(s) 391
BseXI GCAGC 1 cut(s) 172
BshFI GGCC 1 cut(s) 234
BsiHKAI GWGCWC 1 cut(s) 391
BsiHKCI CYCGRG 1 cut(s) 210
BslFI GGGAC 1 cut(s) 304
BslI CCNNNNNNNGG 2 cut(s) 174, 464
BsmAI GTCTC 1 cut(s) 329
BsmFI GGGAC 1 cut(s) 304
BsnI GGCC 1 cut(s) 234
BsoBI CYCGRG 1 cut(s) 210
Bsp1286I GDGCHC 1 cut(s) 391
Bsp143I GATC 1 cut(s) 340
BspANI GGCC 1 cut(s) 234
BspLI GGNNCC 1 cut(s) 36
BspPI GGATC 1 cut(s) 335
BsrI ACTGG 2 cut(s) 38, 308
BssMI GATC 1 cut(s) 340
Bst2UI CCWGG 1 cut(s) 90
Bst6I CTCTTC 1 cut(s) 87
BstBAI YACGTR 1 cut(s) 392
BstF5I GGATG 2 cut(s) 102, 205
BstKTI GATC 1 cut(s) 343
BstMAI GTCTC 1 cut(s) 329
BstMBI GATC 1 cut(s) 340
BstNI CCWGG 1 cut(s) 90
BstSCI CCNGG 1 cut(s) 88
BstSLI GKGCMC 1 cut(s) 391
BstV1I GCAGC 1 cut(s) 172
BstXI CCANNNNNNTGG 1 cut(s) 242
BsuI GTATCC 1 cut(s) 270
BsuRI GGCC 1 cut(s) 234
BtsCI GGATG 2 cut(s) 102, 205
CseI GACGC 1 cut(s) 233
CsiI ACCWGGT 1 cut(s) 88
Csp6I GTAC 3 cut(s) 255, 363, 429
CviAII CATG 2 cut(s) 314, 370
CviJI RGCY 3 cut(s) 163, 234, 325
CviKI_1 RGCY 3 cut(s) 163, 234, 325
CviQI GTAC 3 cut(s) 255, 363, 429
DpnI GATC 1 cut(s) 342
DpnII GATC 1 cut(s) 340
EaeI YGGCCR 1 cut(s) 232
Eam1104I CTCTTC 1 cut(s) 87
EarI CTCTTC 1 cut(s) 87
Eco88I CYCGRG 1 cut(s) 210
EcoRII CCWGG 1 cut(s) 88
EcoT22I ATGCAT 1 cut(s) 315
FaeI CATG 2 cut(s) 317, 373
FaiI YATR 9 cut(s) 32, 152, 270, 315, 321, 371, 379, 395, 448
FaqI GGGAC 1 cut(s) 304
FatI CATG 2 cut(s) 313, 369
Fnu4HI GCNGC 1 cut(s) 161
FokI GGATG 2 cut(s) 89, 192
Fsp4HI GCNGC 1 cut(s) 161
FspBI CTAG 1 cut(s) 475
GluI GCNGC 1 cut(s) 161
HaeIII GGCC 1 cut(s) 234
HgaI GACGC 1 cut(s) 233
Hin1II CATG 2 cut(s) 317, 373
HincII GTYRAC 1 cut(s) 190
HindII GTYRAC 1 cut(s) 190
HinfI GANTC 2 cut(s) 77, 440
HpaI GTTAAC 1 cut(s) 190
Hpy166II GTNNAC 3 cut(s) 190, 389, 439
Hpy188I TCNGA 2 cut(s) 409, 467
Hpy188III TCNNGA 1 cut(s) 212
Hpy8I GTNNAC 3 cut(s) 190, 389, 439
Hpy99I CGWCG 2 cut(s) 116, 227
HpyAV CCTTC 3 cut(s) 266, 281, 367
HpyCH4IV ACGT 1 cut(s) 391
HpyCH4V TGCA 3 cut(s) 68, 313, 389
HpySE526I ACGT 1 cut(s) 391
Hsp92II CATG 2 cut(s) 317, 373
KspAI GTTAAC 1 cut(s) 190
Kzo9I GATC 1 cut(s) 340
LpnPI CCDG 6 cut(s) 51, 75, 102, 160, 212, 289
Lsp1109I GCAGC 1 cut(s) 172
LweI GCATC 1 cut(s) 55
MabI ACCWGGT 1 cut(s) 88
MaeI CTAG 1 cut(s) 475
MaeII ACGT 1 cut(s) 391
MalI GATC 1 cut(s) 342
MboI GATC 1 cut(s) 340
MboII GAAGA 2 cut(s) 17, 74
MfeI CAATTG 1 cut(s) 432
MhlI GDGCHC 1 cut(s) 391
MlsI TGGCCA 1 cut(s) 234
MluCI AATT 5 cut(s) 240, 262, 432, 461, 468
MluNI TGGCCA 1 cut(s) 234
MlyI GAGTC 1 cut(s) 434
MnlI CCTC 2 cut(s) 219, 354
Mox20I TGGCCA 1 cut(s) 234
Mph1103I ATGCAT 1 cut(s) 315
MscI TGGCCA 1 cut(s) 234
MseI TTAA 4 cut(s) 137, 171, 183, 189
MslI CAYNNNNRTG 1 cut(s) 394
Msp20I TGGCCA 1 cut(s) 234
MspR9I CCNGG 1 cut(s) 90
MunI CAATTG 1 cut(s) 432
MvaI CCWGG 1 cut(s) 90
NdeII GATC 1 cut(s) 340
NlaIII CATG 2 cut(s) 317, 373
NlaIV GGNNCC 1 cut(s) 36
NsiI ATGCAT 1 cut(s) 315
OliI CACNNNNGTG 1 cut(s) 394
PaeR7I CTCGAG 1 cut(s) 210
PfeI GAWTC 1 cut(s) 77
PkrI GCNGC 1 cut(s) 162
PleI GAGTC 1 cut(s) 434
PpsI GAGTC 1 cut(s) 434
Ppu21I YACGTR 1 cut(s) 392
Psp6I CCWGG 1 cut(s) 88
PspGI CCWGG 1 cut(s) 88
PspN4I GGNNCC 1 cut(s) 36
RsaI GTAC 3 cut(s) 256, 364, 430
RsaNI GTAC 3 cut(s) 255, 363, 429
RseI CAYNNNNRTG 1 cut(s) 394
SaqAI TTAA 4 cut(s) 137, 171, 183, 189
SatI GCNGC 1 cut(s) 161
Sau3AI GATC 1 cut(s) 340
SchI GAGTC 1 cut(s) 434
ScrFI CCNGG 1 cut(s) 90
SduI GDGCHC 1 cut(s) 391
SetI ASST 8 cut(s) 58, 94, 165, 179, 256, 327, 378, 394
SexAI ACCWGGT 1 cut(s) 88
SfaNI GCATC 1 cut(s) 55
Sfr274I CTCGAG 1 cut(s) 210
SlaI CTCGAG 1 cut(s) 210
SmiMI CAYNNNNRTG 1 cut(s) 394
SmlI CTYRAG 1 cut(s) 210
SmoI CTYRAG 1 cut(s) 210
Sse9I AATT 5 cut(s) 240, 262, 432, 461, 468
SspMI CTAG 1 cut(s) 475
StyD4I CCNGG 1 cut(s) 88
TaiI ACGT 1 cut(s) 394
TaqI TCGA 1 cut(s) 211
TasI AATT 5 cut(s) 240, 262, 432, 461, 468
TfiI GAWTC 1 cut(s) 77
Tru1I TTAA 4 cut(s) 137, 171, 183, 189
Tru9I TTAA 4 cut(s) 137, 171, 183, 189
TseI GCWGC 1 cut(s) 160
TspDTI ATGAA 6 cut(s) 63, 91, 167, 285, 330, 386
VneI GTGCAC 1 cut(s) 387
XapI RAATTY 1 cut(s) 468
XhoI CTCGAG 1 cut(s) 210
XspI CTAG 1 cut(s) 475
Zsp2I ATGCAT 1 cut(s) 315
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.