Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0000987

Physical Location & Seq

Genomic Coordinates Forward (+)

12439867 .. 12441969

Gene Structure 2103 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1165300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1560 bp

ATGGCCATGACTTCAGCTTTCCGCTGCCGCACACTTTTTTGGCTAATTATCTTCTTTTTTCTCGTTTTCTTCGTTCTAACCTTCTCACCCTTGCTTCGTGAAAACAATGTCATTATTCCCTTTGTTTCCTCTTTCAATAGTACTACTACACCTTTCAATCTGACAAAGAGCAACTGCACATCCCTACCTCAAGTTTCATATTTTGATGAGCTTCTCACAAGCTCCATGTATCAAAGGAGACACAAGGCTAATCTTACCAAAGTAAGCATTTCAATTTCATTTTTCAGGTTAATAACAGTTTCAATTTTCAAAACTTGTTTAGTAACTGTTTTCATTTTTCAAAAAGTGACCAAGCCAAATTTGGAAAATTCATCACAAAAGAGAGCTTTATGTTTTAATTATTTTGAACAAAATGATTATGAAATGACCCCCAAGTGTAATCGTTCTCATGTAGTTATTAGAATCAAAACGATGGGTAAGACTAGTTTGGATACAATTGAAGAAGGCTTGGCCAAAGCACGAGCTGCAATTCTTGAAGCAATTCAGTTTAAAAAGTATGCATCACAGAAAAATGAGACTTTTATCCCAAGAGGAACCATTTACAAAAATCCCTATGCTTTTCACCAGAGTCACGTAGAGATGATGAAGAGGTTCAAGGTGTGGAGCTACAAGGAAGGAGAGCATCCATTAATACATTTTGGTCCAGTGAATGACATATATGGAATCGAGGGACAATTCATTGATGAAATAGAGAGGGAGGAAAGCCCATTCAGGGCTACACATCCTGATGAGGCACACACATTTTTCCTTCCCTTCAGTGTTGCCAACGTTGTTCGCTATGTCTACATGCCTATCACTCGGAAGCAAGATTACCATCGCGACCGCCTGCAACGGGTTGTCACCGACAACATCAGCGTTGTGGCTGATAAATATCCCTATTGGAACAGAAGCAATGGTGCTGACCATTTTATGGCTTCATGTCATGATTGGGTACATGTTTCACTGCCTAAAATCTACTTACCCAAAGGAAATTTGGACCCACCCAATTTGGGTCAGGCCCCAAAGAGCCGCCCAATTCTAGCATTCTTTGCCGGTCGGGTTCATGGACCTGTTCGGCCCATCTTGCTGGATCATTGGAAGGACAAAGATGATGAAGTTCAAGTCTACGAGAAGCTTCCCAAGGGCCTAAACTACACCAAATTTATGGGGCAGAGCAAATTTTGCTTGTGCCCTAGTGGATTTGAAGTTGCAAGCCCAAGAATTGTGGAGGCAATCTATGCAGGGTGTGTGCCAGTCTTAATTTCTGACAATTACACTTTGCTCTTCAGTGATGTGCTTGATTGGAGCAAATTTTCAGTGCAAATTCCAGTTGATAAGATACCAGAAATTAAGACCATCTTACAAGCAATTCCATTCGACAAATATCTGAAAATGCAGAAGAGGGTGTATAGGGTTCGTAGGCATTTTGTGCTAAACAGGCCATCTAAACCGTTTGATGTAATTCACATGGTGCTTCACTCAGTATGGTTAAGAAGACTTAACTTTAAACTCGAGAATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000139 GO:0000271 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0005975 GO:0005976 GO:0008150 GO:0008152 GO:0008194 GO:0009058 GO:0009059 GO:0009987 GO:0010393 GO:0010398 GO:0012505 GO:0016020 GO:0016051 GO:0016740 GO:0016757 GO:0016763 GO:0017144 GO:0031090 GO:0031410 GO:0031982 GO:0031984 GO:0035252 GO:0042285 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045488 GO:0045489 GO:0071704 GO:0097708 GO:0098588 GO:0098791 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Exostosin

Protein Analysis

520

Amino Acids

60.53

Weight (kDa)

9.47

Isoelectric Point (pI)

42.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Exostosin_GT47	PF03016	200 - 330	1e-14	Exostosin GT47 domain
Exostosin_GT47	PF03016	350 - 470	8e-34	Exostosin GT47 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000987)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G42180 AT3G42180
fragaria_vesca	FvH4_1g02510 FvH4_1g02510 FvH4_1g02511 FvH4_1g02520 FvH4_1g02520 FvH4_6g00700 FvH4_6g00700 FvH4_6g00700
malus_domestica	MD02G1022600.v1.1 MD04G1242600.v1.1 MD04G1243200.v1.1 MD04G1243300.v1.1 MD04G1243400.v1.1 MD15G1165200.v1.1 MD15G1165300.v1.1
prunus_persica	Prupe.6G359500_v2.0.a1 Prupe.6G359600_v2.0.a1 Prupe.6G359600_v2.0.a1 Prupe.7G249500_v2.0.a1 Prupe.7G249600_v2.0.a1 Prupe.7G249600_v2.0.a1
pyrus_communis	pycom04g21420
rosa_chinensis	RchiOBHm_Chr2g0087521 RchiOBHm_Chr2g0087541 RchiOBHm_Chr2g0087551 RchiOBHm_Chr3g0448141 RchiOBHm_Chr3g0448161
rosa_laevigata	RLG00000015875 RLG00000015876 RLG00000015877 RLG00000025942 RLG00000025943
rosa_multiflora	Rmu_sc0002299.1_g000012 Rmu_sc0002299.1_g000014 Rmu_sc0002299.1_g000020 Rmu_sc0005969.1_g000031
rosa_roxburghii	Rroxscaffold_2G00153480 Rroxscaffold_6G00426270 Rroxscaffold_6G00426300
rosa_rugosa	Rorug01G0476400 Rorug01G0476400 Rorug01G0476400 Rorug02G0608400 Rorug02G0608400 Rorug02G0608500
rosa_samantha	Rh2AG029600 Rh2AG029700 Rh2AG029800 Rh2BG029700 Rh2BG029800 Rh3BG007800 Rh3BG007900 Rh3CG007800 Rh3CG008000 Rh3DG008200
rosa_wichuraiana	Rw2G002350 Rw2G002360 Rw2G002370 Rw3G000650 Rw3G000660

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	970
AccI	GTMKAC	2 cut(s)	843, 1164
AccII	CGCG	1 cut(s)	879
AciI	CCGC	4 cut(s)	22, 28, 883, 1069
AclI	AACGTT	1 cut(s)	828
AclWI	GGATC	1 cut(s)	1137
AcoI	YGGCCR	2 cut(s)	3, 510
AcsI	RAATTY	7 cut(s)	358, 367, 1030, 1199, 1217, 1349, 1362
AcuI	CTGAAG	2 cut(s)	799, 1309
AdeI	CACNNNGTG	1 cut(s)	1510
AfaI	GTAC	2 cut(s)	142, 993
AfiI	CCNNNNNNNGG	4 cut(s)	772, 892, 970, 1049
AflIII	ACRYGT	1 cut(s)	994
AhlI	ACTAGT	1 cut(s)	482
AluBI	AGCT	7 cut(s)	17, 211, 222, 386, 524, 666, 1174
AluI	AGCT	7 cut(s)	17, 211, 222, 386, 524, 666, 1174
Alw26I	GTCTC	2 cut(s)	232, 569
AlwI	GGATC	1 cut(s)	1137
Ama87I	CYCGRG	1 cut(s)	1550
AoxI	GGCC	6 cut(s)	3, 510, 1056, 1115, 1183, 1478
ApeKI	GCWGC	2 cut(s)	24, 524
ApoI	RAATTY	7 cut(s)	358, 367, 1030, 1199, 1217, 1349, 1362
AseI	ATTAAT	1 cut(s)	689
Asp700I	GAANNNNTTC	2 cut(s)	540, 650
AspS9I	GGNCC	6 cut(s)	701, 1036, 1057, 1106, 1116, 1183
AsuHPI	GGTGA	3 cut(s)	78, 614, 892
AvaI	CYCGRG	1 cut(s)	1550
AvaII	GGWCC	3 cut(s)	701, 1036, 1106
BaeGI	GKGCMC	1 cut(s)	1232
BalI	TGGCCA	2 cut(s)	5, 512
BarI	GAAGNNNNNNTAC	2 cut(s)	854, 886
BauI	CACGAG	1 cut(s)	519
BbsI	GAAGAC	1 cut(s)	1540
BbvI	GCAGC	2 cut(s)	11, 511
BccI	CCATC	5 cut(s)	466, 882, 1127, 1403, 1489
BciVI	GTATCC	1 cut(s)	484
BcoDI	GTCTC	2 cut(s)	232, 569
BcuI	ACTAGT	1 cut(s)	482
BfaI	CTAG	3 cut(s)	483, 1079, 1233
BfuI	GTATCC	1 cut(s)	484
BisI	GCNGC	4 cut(s)	25, 28, 525, 1069
BlsI	GCNGC	4 cut(s)	26, 29, 526, 1070
BmcAI	AGTACT	1 cut(s)	142
Bme18I	GGWCC	3 cut(s)	701, 1036, 1106
BmeT110I	CYCGRG	1 cut(s)	1550
BmgT120I	GGNCC	6 cut(s)	701, 1036, 1057, 1106, 1116, 1183
BmiI	GGNNCC	3 cut(s)	595, 1038, 1059
BmsI	GCATC	2 cut(s)	569, 691
BpiI	GAAGAC	1 cut(s)	1540
BpuEI	CTTGAG	1 cut(s)	174
BsaAI	YACGTR	1 cut(s)	634
BsaBI	GATNNNNATC	2 cut(s)	873, 930
BsaJI	CCNNGG	1 cut(s)	1179
Bsc4I	CCNNNNNNNGG	4 cut(s)	772, 892, 970, 1049
Bse118I	RCCGGY	1 cut(s)	1091
Bse1I	ACTGG	3 cut(s)	704, 1292, 1367
Bse3DI	GCAATG	1 cut(s)	958
Bse8I	GATNNNNATC	2 cut(s)	873, 930
BseDI	CCNNGG	1 cut(s)	1179
BseGI	GGATG	3 cut(s)	179, 682, 781
BseJI	GATNNNNATC	2 cut(s)	873, 930
BseLI	CCNNNNNNNGG	4 cut(s)	772, 892, 970, 1049
BseMI	GCAATG	1 cut(s)	958
BseMII	CTCAG	1 cut(s)	1533
BseNI	ACTGG	3 cut(s)	704, 1292, 1367
BseSI	GKGCMC	1 cut(s)	1232
BseXI	GCAGC	2 cut(s)	11, 511
BsgI	GTGCAG	1 cut(s)	160
Bsh1236I	CGCG	1 cut(s)	879
Bsh1285I	CGRYCG	2 cut(s)	883, 1096
BshFI	GGCC	6 cut(s)	5, 512, 1058, 1117, 1185, 1480
BsiEI	CGRYCG	2 cut(s)	883, 1096
BsiHKCI	CYCGRG	1 cut(s)	1550
BsiSI	CCGG	1 cut(s)	1092
BslFI	GGGAC	1 cut(s)	744
BslI	CCNNNNNNNGG	4 cut(s)	772, 892, 970, 1049
BsmAI	GTCTC	2 cut(s)	232, 569
BsmFI	GGGAC	1 cut(s)	744
BsmI	GAATGC	1 cut(s)	1082
BsnI	GGCC	6 cut(s)	5, 512, 1058, 1117, 1185, 1480
BsoBI	CYCGRG	1 cut(s)	1550
Bsp1286I	GDGCHC	1 cut(s)	1232
Bsp143I	GATC	1 cut(s)	1129
Bsp68I	TCGCGA	1 cut(s)	879
BspACI	CCGC	4 cut(s)	22, 28, 883, 1069
BspANI	GGCC	6 cut(s)	5, 512, 1058, 1117, 1185, 1480
BspCNI	CTCAG	1 cut(s)	1532
BspFNI	CGCG	1 cut(s)	879
BspHI	TCATGA	1 cut(s)	982
BspLI	GGNNCC	3 cut(s)	595, 1038, 1059
BspPI	GGATC	1 cut(s)	1137
BspQI	GCTCTTC	1 cut(s)	1328
BsrDI	GCAATG	1 cut(s)	958
BsrFI	RCCGGY	1 cut(s)	1091
BsrI	ACTGG	3 cut(s)	704, 1292, 1367
BssAI	RCCGGY	1 cut(s)	1091
BssECI	CCNNGG	1 cut(s)	1179
BssMI	GATC	1 cut(s)	1129
BssSI	CACGAG	1 cut(s)	519
BssT1I	CCWWGG	1 cut(s)	1179
Bst2BI	CACGAG	1 cut(s)	519
Bst4CI	ACNGT	3 cut(s)	298, 328, 1491
Bst6I	CTCTTC	3 cut(s)	641, 1328, 1433
BstAPI	GCANNNNNTGC	5 cut(s)	524, 1088, 1221, 1277, 1468
BstBAI	YACGTR	1 cut(s)	634
BstC8I	GCNNGC	2 cut(s)	887, 1252
BstDEI	CTNAG	1 cut(s)	1519
BstF5I	GGATG	3 cut(s)	179, 682, 781
BstFNI	CGCG	1 cut(s)	879
BstKTI	GATC	1 cut(s)	1132
BstMAI	GTCTC	2 cut(s)	232, 569
BstMBI	GATC	1 cut(s)	1129
BstMCI	CGRYCG	2 cut(s)	883, 1096
BstMWI	GCNNNNNNNGC	7 cut(s)	524, 1088, 1123, 1221, 1277, 1468, 1477
BstNSI	RCATGY	2 cut(s)	850, 998
BstSLI	GKGCMC	1 cut(s)	1232
BstUI	CGCG	1 cut(s)	879
BstV1I	GCAGC	2 cut(s)	11, 511
BstV2I	GAAGAC	1 cut(s)	1540
BstXI	CCANNNNNNTGG	2 cut(s)	1126, 1204
BsuI	GTATCC	1 cut(s)	484
BsuRI	GGCC	6 cut(s)	5, 512, 1058, 1117, 1185, 1480
BtgZI	GCGATG	1 cut(s)	860
BtsCI	GGATG	3 cut(s)	179, 682, 781
BtsI	GCAGTG	1 cut(s)	1001
BtsIMutI	CAGTG	5 cut(s)	711, 823, 1001, 1333, 1362
BtuMI	TCGCGA	1 cut(s)	879
Cac8I	GCNNGC	2 cut(s)	887, 1252
CciI	TCATGA	1 cut(s)	982
Cfr10I	RCCGGY	1 cut(s)	1091
Cfr13I	GGNCC	6 cut(s)	701, 1036, 1057, 1106, 1116, 1183
Csp6I	GTAC	2 cut(s)	141, 992
CspCI	CAANNNNNGTGG	4 cut(s)	1029, 1064, 1245, 1280
CviAII	CATG	9 cut(s)	7, 226, 449, 847, 978, 983, 995, 1103, 1507
CviQI	GTAC	2 cut(s)	141, 992
DdeI	CTNAG	1 cut(s)	1519
DpnI	GATC	1 cut(s)	1131
DpnII	GATC	1 cut(s)	1129
DraI	TTTAAA	2 cut(s)	550, 1546
DraIII	CACNNNGTG	1 cut(s)	1510
EaeI	YGGCCR	2 cut(s)	3, 510
Eam1104I	CTCTTC	3 cut(s)	641, 1328, 1433
EarI	CTCTTC	3 cut(s)	641, 1328, 1433
Eco130I	CCWWGG	1 cut(s)	1179
Eco47I	GGWCC	3 cut(s)	701, 1036, 1106
Eco57I	CTGAAG	2 cut(s)	799, 1309
Eco88I	CYCGRG	1 cut(s)	1550
EcoO109I	RGGNCCY	2 cut(s)	1057, 1183
EcoT14I	CCWWGG	1 cut(s)	1179
EcoT22I	ATGCAT	1 cut(s)	562
ErhI	CCWWGG	1 cut(s)	1179
FaeI	CATG	9 cut(s)	10, 229, 452, 850, 981, 986, 998, 1106, 1510
FalI	AAGNNNNNCTT	2 cut(s)	1382, 1414
FaqI	GGGAC	1 cut(s)	744
FatI	CATG	9 cut(s)	6, 225, 448, 846, 977, 982, 994, 1102, 1506
FblI	GTMKAC	2 cut(s)	843, 1164
Fnu4HI	GCNGC	4 cut(s)	25, 28, 525, 1069
FokI	GGATG	3 cut(s)	166, 669, 768
Fsp4HI	GCNGC	4 cut(s)	25, 28, 525, 1069
FspBI	CTAG	3 cut(s)	483, 1079, 1233
GluI	GCNGC	4 cut(s)	25, 28, 525, 1069
HaeIII	GGCC	6 cut(s)	5, 512, 1058, 1117, 1185, 1480
HapII	CCGG	1 cut(s)	1092
Hin1II	CATG	9 cut(s)	10, 229, 452, 850, 981, 986, 998, 1106, 1510
HindIII	AAGCTT	1 cut(s)	1172
HinfI	GANTC	3 cut(s)	462, 628, 723
HpaII	CCGG	1 cut(s)	1092
HphI	GGTGA	3 cut(s)	78, 614, 892
Hpy166II	GTNNAC	2 cut(s)	844, 1165
Hpy188I	TCNGA	4 cut(s)	162, 861, 1306, 1428
Hpy188III	TCNNGA	6 cut(s)	98, 533, 785, 878, 983, 1552
Hpy8I	GTNNAC	2 cut(s)	844, 1165
HpyAV	CCTTC	6 cut(s)	91, 497, 668, 818, 823, 1132
HpyCH4III	ACNGT	3 cut(s)	298, 328, 1491
HpyCH4IV	ACGT	2 cut(s)	633, 828
HpyCH4V	TGCA	8 cut(s)	177, 527, 560, 889, 1250, 1280, 1360, 1435
HpyF10VI	GCNNNNNNNGC	7 cut(s)	524, 1088, 1123, 1221, 1277, 1468, 1477
HpyF3I	CTNAG	1 cut(s)	1519
HpySE526I	ACGT	2 cut(s)	633, 828
Hsp92II	CATG	9 cut(s)	10, 229, 452, 850, 981, 986, 998, 1106, 1510
Kzo9I	GATC	1 cut(s)	1129
LguI	GCTCTTC	1 cut(s)	1328
LmnI	GCTCC	3 cut(s)	227, 663, 1344
Lsp1109I	GCAGC	2 cut(s)	11, 511
LweI	GCATC	2 cut(s)	569, 691
MaeI	CTAG	3 cut(s)	483, 1079, 1233
MaeII	ACGT	2 cut(s)	633, 828
MaeIII	GTNAC	4 cut(s)	322, 346, 629, 898
MalI	GATC	1 cut(s)	1131
MboI	GATC	1 cut(s)	1129
MboII	GAAGA	7 cut(s)	43, 61, 512, 658, 1315, 1450, 1545
MfeI	CAATTG	1 cut(s)	495
MhlI	GDGCHC	1 cut(s)	1232
MlsI	TGGCCA	2 cut(s)	5, 512
MluNI	TGGCCA	2 cut(s)	5, 512
MlyI	GAGTC	1 cut(s)	637
Mox20I	TGGCCA	2 cut(s)	5, 512
Mph1103I	ATGCAT	1 cut(s)	562
MroXI	GAANNNNTTC	2 cut(s)	540, 650
MscI	TGGCCA	2 cut(s)	5, 512
MslI	CAYNNNNRTG	1 cut(s)	786
Msp20I	TGGCCA	2 cut(s)	5, 512
MspA1I	CMGCKG	1 cut(s)	24
MspI	CCGG	1 cut(s)	1092
MunI	CAATTG	1 cut(s)	495
Mva1269I	GAATGC	1 cut(s)	1082
MvnI	CGCG	1 cut(s)	879
MwoI	GCNNNNNNNGC	7 cut(s)	524, 1088, 1123, 1221, 1277, 1468, 1477
NdeII	GATC	1 cut(s)	1129
NlaIII	CATG	9 cut(s)	10, 229, 452, 850, 981, 986, 998, 1106, 1510
NlaIV	GGNNCC	3 cut(s)	595, 1038, 1059
NmuCI	GTSAC	3 cut(s)	346, 629, 898
NruI	TCGCGA	1 cut(s)	879
NsiI	ATGCAT	1 cut(s)	562
NspI	RCATGY	2 cut(s)	850, 998
PaeR7I	CTCGAG	1 cut(s)	1550
PagI	TCATGA	1 cut(s)	982
PciI	ACATGT	1 cut(s)	994
PciSI	GCTCTTC	1 cut(s)	1328
PcsI	WCGNNNNNNNCGW	1 cut(s)	69
PctI	GAATGC	1 cut(s)	1082
PdmI	GAANNNNTTC	2 cut(s)	540, 650
PfeI	GAWTC	2 cut(s)	462, 723
PflMI	CCANNNNNTGG	1 cut(s)	970
PkrI	GCNGC	4 cut(s)	26, 29, 526, 1070
PleI	GAGTC	1 cut(s)	636
PpsI	GAGTC	1 cut(s)	636
Ppu21I	YACGTR	1 cut(s)	634
PscI	ACATGT	1 cut(s)	994
PshBI	ATTAAT	1 cut(s)	689
Psp1406I	AACGTT	1 cut(s)	828
PspN4I	GGNNCC	3 cut(s)	595, 1038, 1059
PspPI	GGNCC	6 cut(s)	701, 1036, 1057, 1106, 1116, 1183
RruI	TCGCGA	1 cut(s)	879
RsaI	GTAC	2 cut(s)	142, 993
RsaNI	GTAC	2 cut(s)	141, 992
RseI	CAYNNNNRTG	1 cut(s)	786
SapI	GCTCTTC	1 cut(s)	1328
SatI	GCNGC	4 cut(s)	25, 28, 525, 1069
Sau3AI	GATC	1 cut(s)	1129
Sau96I	GGNCC	6 cut(s)	701, 1036, 1057, 1106, 1116, 1183
ScaI	AGTACT	1 cut(s)	142
SchI	GAGTC	1 cut(s)	637
SduI	GDGCHC	1 cut(s)	1232
SfaNI	GCATC	2 cut(s)	569, 691
Sfr274I	CTCGAG	1 cut(s)	1550
SinI	GGWCC	3 cut(s)	701, 1036, 1106
SlaI	CTCGAG	1 cut(s)	1550
SmiMI	CAYNNNNRTG	1 cut(s)	786
SmlI	CTYRAG	2 cut(s)	189, 1550
SmoI	CTYRAG	2 cut(s)	189, 1550
SpeI	ACTAGT	1 cut(s)	482
SsiI	CCGC	4 cut(s)	22, 28, 883, 1069
SspMI	CTAG	3 cut(s)	483, 1079, 1233
StyI	CCWWGG	1 cut(s)	1179
TaaI	ACNGT	3 cut(s)	298, 328, 1491
TaiI	ACGT	2 cut(s)	636, 831
TaqI	TCGA	3 cut(s)	726, 1416, 1551
TatI	WGTACW	1 cut(s)	140
TauI	GCSGC	2 cut(s)	30, 1071
TfiI	GAWTC	2 cut(s)	462, 723
TscAI	CASTG	5 cut(s)	711, 823, 1008, 1333, 1362
TseFI	GTSAC	3 cut(s)	346, 629, 898
TseI	GCWGC	2 cut(s)	24, 524
Tsp45I	GTSAC	3 cut(s)	346, 629, 898
TspRI	CASTG	5 cut(s)	711, 823, 1008, 1333, 1362
Van91I	CCANNNNNTGG	1 cut(s)	970
VpaK11BI	GGWCC	3 cut(s)	701, 1036, 1106
VspI	ATTAAT	1 cut(s)	689
XapI	RAATTY	7 cut(s)	358, 367, 1030, 1199, 1217, 1349, 1362
XceI	RCATGY	2 cut(s)	850, 998
XcmI	CCANNNNNNNNNTGG	2 cut(s)	358, 1030
XhoI	CTCGAG	1 cut(s)	1550
XmiI	GTMKAC	2 cut(s)	843, 1164
XmnI	GAANNNNTTC	2 cut(s)	540, 650
XspI	CTAG	3 cut(s)	483, 1079, 1233
ZrmI	AGTACT	1 cut(s)	142
Zsp2I	ATGCAT	1 cut(s)	562

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.