Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0000987

Physical Location & Seq

Genomic Coordinates Forward (+)

2230481 .. 2232195

Gene Structure 1715 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ46298

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1446 bp

ATGACTCGAAGCTTTCGTTGCCCTGCACTTTTTTTGTTAACTACATTGTCTTGTCTTCTGTTCTTCCACCTAATCTTCACACCTTTATCGTCTGAAACCCATCTTAGTATTTCTCCAGTATCCTCTTTCACTACACCTTTGACTAATCCAGAAAAGACCAGCAACTCAACTCCATCGAAACATGTAGCTTCTTTAGATGAGGTTCTCACCAGCTCCATGTACCGAAGGATTAGGAGCAAGGTTGACGGTACCAAAGAGAAGACTAGTTTGGAGAGAATTGAAGAAGATTTAGCTAAAGCACGAGCATCGATTCTCAGAGCAATTCAGTTTGAAAACTACACATCAGAGAAAGTACAGAGTTTTGTCCCAAGAGGAAGCATTTACAAAAATCCTTATGCTTTTCACCAGTTGAGTTTTACGAGTCACCTAGAAATGATGAAGAGGTTCAAGATGTGGAGCTATGAGGAAGGAGAACAACCCTTGGTCCATTTTGGTCCTATGAATGATATATATGGCATTGAAGGACACTTCATAGATGAAATAGAAAGGGAGGGGAGCCCATTTAGGGCTACACATCCTGATGAGGCACACATGTTTTTCCTCCCATTTAGTGTAGCTAACATTGTTCACTATGTTTACTTGCCTATCACTAAAAAACAGGACTACCACCGTGATCGTCTACAACAGATTACCAGGGACTACATTGGCGTTGTGGCACATAAGTATCCCTATTGGAATAGGAGCAAAGGTGCCGACCATTTTATGGCTTCATGTCACGATTGGGCACCAGAGATTTCAGCTGGCCAATCGAAGCTTTTCAAAAACTTCATTAGAGTGCTTTGCAACGCCAACACCTCTGAAGGGTTCCAACCTAAGAGAGATGTTCCATTACCTGAAATATTTGTACCTTCTGGAAAGCTCGGTCCCCCCAATTTGGGTCAGGCTCCAAACAATCGTCGAATTCTGGCCTTCTTTGCTGGTAGAGTTCATGGACCGATTCGACCAATCTTGCTCGAGCATTGGAAGGACAAAGATGACGAAATTCAAGTCCACGAGAAGCTTCCCAAGGGCATGAACTACACGGCATTAATGGGACAAAGCAAGTTTTGCTTGTGCCCTAGTGGTTTTGAAGTGGCAAGCCCTAGAGTTGTGGAGGCATTTTATGCAGGGTGTGTTCCGGTGTTAATTAACGACAACTACACATTACCCTTTAGTGATGTCCTTGATTGGAGCCAATTTTCAATACAAATTCCCGTCGCGAAAATACAAGAAATCAAGACCATCTTACAAGCAATTCCAAATGAGGAATACCAAAAGATGCAGAGGAGGGTTTTAAAAGTTCGGAGGCATTTTGTTCTCAACAAGCCGGCTAAGCCATTTGATGTGATTCACATGGTACTTCATTCAGTGTGGTTGAGACGACTTAATTTTAAGCTTGGAACTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000139 GO:0000271 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0005975 GO:0005976 GO:0008150 GO:0008152 GO:0008194 GO:0009058 GO:0009059 GO:0009987 GO:0010393 GO:0010398 GO:0012505 GO:0016020 GO:0016051 GO:0016740 GO:0016757 GO:0016763 GO:0017144 GO:0031090 GO:0031410 GO:0031982 GO:0031984 GO:0035252 GO:0042285 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045488 GO:0045489 GO:0071704 GO:0097708 GO:0098588 GO:0098791 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Exostosin

Protein Analysis

481

Amino Acids

55.38

Weight (kDa)

9.16

Isoelectric Point (pI)

53.31

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Exostosin_GT47	PF03016	145 - 432	7.6e-61	Exostosin GT47 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000987)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G42180 AT3G42180
fragaria_vesca	FvH4_1g02510 FvH4_1g02510 FvH4_1g02511 FvH4_1g02520 FvH4_1g02520 FvH4_6g00700 FvH4_6g00700 FvH4_6g00700
malus_domestica	MD02G1022600.v1.1 MD04G1242600.v1.1 MD04G1243200.v1.1 MD04G1243300.v1.1 MD04G1243400.v1.1 MD15G1165200.v1.1 MD15G1165300.v1.1
prunus_persica	Prupe.6G359500_v2.0.a1 Prupe.6G359600_v2.0.a1 Prupe.6G359600_v2.0.a1 Prupe.7G249500_v2.0.a1 Prupe.7G249600_v2.0.a1 Prupe.7G249600_v2.0.a1
pyrus_communis	pycom04g21420
rosa_chinensis	RchiOBHm_Chr2g0087521 RchiOBHm_Chr2g0087541 RchiOBHm_Chr2g0087551 RchiOBHm_Chr3g0448141 RchiOBHm_Chr3g0448161
rosa_laevigata	RLG00000015875 RLG00000015876 RLG00000015877 RLG00000025942 RLG00000025943
rosa_multiflora	Rmu_sc0002299.1_g000012 Rmu_sc0002299.1_g000014 Rmu_sc0002299.1_g000020 Rmu_sc0005969.1_g000031
rosa_roxburghii	Rroxscaffold_2G00153480 Rroxscaffold_6G00426270 Rroxscaffold_6G00426300
rosa_rugosa	Rorug01G0476400 Rorug01G0476400 Rorug01G0476400 Rorug02G0608400 Rorug02G0608400 Rorug02G0608500
rosa_samantha	Rh2AG029600 Rh2AG029700 Rh2AG029800 Rh2BG029700 Rh2BG029800 Rh3BG007800 Rh3BG007900 Rh3CG007800 Rh3CG008000 Rh3DG008200
rosa_wichuraiana	Rw2G002350 Rw2G002360 Rw2G002370 Rw3G000650 Rw3G000660

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	248
AccB1I	GGYRCC	3 cut(s)	248, 749, 784
AccB7I	CCANNNNNTGG	1 cut(s)	763
AccI	GTMKAC	1 cut(s)	679
AccII	CGCG	1 cut(s)	1259
AcoI	YGGCCR	1 cut(s)	802
AcsI	RAATTY	3 cut(s)	960, 1041, 1248
AcuI	CTGAAG	1 cut(s)	879
AfaI	GTAC	5 cut(s)	221, 250, 354, 906, 1398
AfiI	CCNNNNNNNGG	5 cut(s)	565, 763, 861, 934, 935
AflIII	ACRYGT	2 cut(s)	181, 591
AgsI	TTSAA	8 cut(s)	281, 332, 448, 521, 820, 1046, 1130, 1242
AhlI	ACTAGT	1 cut(s)	263
AjnI	CCWGG	1 cut(s)	692
AjuI	GAANNNNNNNTTGG	2 cut(s)	251, 283
Alw26I	GTCTC	1 cut(s)	1411
Ama87I	CYCGRG	1 cut(s)	1013
AoxI	GGCC	2 cut(s)	802, 966
ApoI	RAATTY	3 cut(s)	960, 1041, 1248
ArsI	GACNNNNNNTTYG	2 cut(s)	1032, 1064
AseI	ATTAAT	1 cut(s)	1088
Asp700I	GAANNNNTTC	2 cut(s)	443, 815
Asp718I	GGTACC	1 cut(s)	248
AspS9I	GGNCC	4 cut(s)	484, 494, 923, 992
AsuHPI	GGTGA	3 cut(s)	199, 395, 416
AvaI	CYCGRG	1 cut(s)	1013
AvaII	GGWCC	4 cut(s)	484, 494, 923, 992
BaeGI	GKGCMC	2 cut(s)	787, 1118
BaeI	ACNNNNGTAYC	2 cut(s)	707, 740
BalI	TGGCCA	1 cut(s)	804
BanI	GGYRCC	3 cut(s)	248, 749, 784
BanII	GRGCYC	1 cut(s)	560
BauI	CACGAG	2 cut(s)	300, 1052
BbsI	GAAGAC	2 cut(s)	47, 266
BccI	CCATC	3 cut(s)	108, 181, 1289
BceAI	ACGGC	1 cut(s)	1098
BcgI	CGANNNNNNTGC	2 cut(s)	288, 322
BciT130I	CCWGG	1 cut(s)	694
BciVI	GTATCC	2 cut(s)	130, 735
BcoDI	GTCTC	1 cut(s)	1411
BcuI	ACTAGT	1 cut(s)	263
BfaI	CTAG	4 cut(s)	264, 428, 1119, 1143
BfuI	GTATCC	2 cut(s)	130, 735
BlpI	GCTNAGC	1 cut(s)	1371
Bme1390I	CCNGG	1 cut(s)	694
Bme18I	GGWCC	4 cut(s)	484, 494, 923, 992
BmeT110I	CYCGRG	1 cut(s)	1013
BmgT120I	GGNCC	4 cut(s)	484, 494, 923, 992
BmiI	GGNNCC	8 cut(s)	250, 557, 751, 786, 866, 925, 945, 1232
BmrFI	CCNGG	1 cut(s)	694
BmsI	GCATC	2 cut(s)	314, 1308
BpiI	GAAGAC	2 cut(s)	47, 266
BpmI	CTGGAG	1 cut(s)	99
Bpu1102I	GCTNAGC	1 cut(s)	1371
Bsa29I	ATCGAT	1 cut(s)	308
BsaJI	CCNNGG	3 cut(s)	480, 693, 1065
BsaWI	WCCGGW	1 cut(s)	1177
BsaXI	ACNNNNNCTCC	2 cut(s)	733, 763
Bsc4I	CCNNNNNNNGG	5 cut(s)	565, 763, 861, 934, 935
Bse118I	RCCGGY	1 cut(s)	1366
Bse1I	ACTGG	2 cut(s)	116, 406
BseBI	CCWGG	1 cut(s)	694
BseCI	ATCGAT	1 cut(s)	308
BseDI	CCNNGG	3 cut(s)	480, 693, 1065
BseGI	GGATG	1 cut(s)	574
BseLI	CCNNNNNNNGG	5 cut(s)	565, 763, 861, 934, 935
BseMII	CTCAG	1 cut(s)	328
BseNI	ACTGG	2 cut(s)	116, 406
BseRI	GAGGAG	1 cut(s)	1339
BseSI	GKGCMC	2 cut(s)	787, 1118
BsgI	GTGCAG	1 cut(s)	9
Bsh1236I	CGCG	1 cut(s)	1259
BshFI	GGCC	2 cut(s)	804, 968
BshNI	GGYRCC	3 cut(s)	248, 749, 784
BshVI	ATCGAT	1 cut(s)	308
BsiHKCI	CYCGRG	1 cut(s)	1013
BsiSI	CCGG	2 cut(s)	1178, 1367
BslFI	GGGAC	4 cut(s)	350, 710, 909, 1107
BslI	CCNNNNNNNGG	5 cut(s)	565, 763, 861, 934, 935
BsmAI	GTCTC	1 cut(s)	1411
BsmBI	CGTCTC	1 cut(s)	1411
BsmFI	GGGAC	4 cut(s)	350, 710, 909, 1107
BsnI	GGCC	2 cut(s)	804, 968
BsoBI	CYCGRG	1 cut(s)	1013
Bsp1286I	GDGCHC	3 cut(s)	560, 787, 1118
Bsp143I	GATC	1 cut(s)	673
Bsp1720I	GCTNAGC	1 cut(s)	1371
Bsp68I	TCGCGA	1 cut(s)	1259
BspANI	GGCC	2 cut(s)	804, 968
BspCNI	CTCAG	1 cut(s)	327
BspDI	ATCGAT	1 cut(s)	308
BspFNI	CGCG	1 cut(s)	1259
BspLI	GGNNCC	8 cut(s)	250, 557, 751, 786, 866, 925, 945, 1232
BspT107I	GGYRCC	3 cut(s)	248, 749, 784
BsrFI	RCCGGY	1 cut(s)	1366
BsrI	ACTGG	2 cut(s)	116, 406
BssAI	RCCGGY	1 cut(s)	1366
BssECI	CCNNGG	3 cut(s)	480, 693, 1065
BssMI	GATC	1 cut(s)	673
BssSI	CACGAG	2 cut(s)	300, 1052
BssT1I	CCWWGG	2 cut(s)	480, 1065
Bst2BI	CACGAG	2 cut(s)	300, 1052
Bst2UI	CCWGG	1 cut(s)	694
Bst4CI	ACNGT	2 cut(s)	248, 671
Bst6I	CTCTTC	1 cut(s)	434
BstAPI	GCANNNNNTGC	2 cut(s)	1107, 1163
BstC8I	GCNNGC	3 cut(s)	802, 1138, 1368
BstDEI	CTNAG	4 cut(s)	104, 314, 873, 1371
BstF5I	GGATG	1 cut(s)	574
BstFNI	CGCG	1 cut(s)	1259
BstKTI	GATC	1 cut(s)	676
BstMAI	GTCTC	1 cut(s)	1411
BstMBI	GATC	1 cut(s)	673
BstMWI	GCNNNNNNNGC	5 cut(s)	18, 974, 1107, 1163, 1372
BstNI	CCWGG	1 cut(s)	694
BstNSI	RCATGY	2 cut(s)	185, 595
BstSCI	CCNGG	1 cut(s)	692
BstSLI	GKGCMC	2 cut(s)	787, 1118
BstUI	CGCG	1 cut(s)	1259
BstV2I	GAAGAC	2 cut(s)	47, 266
Bsu15I	ATCGAT	1 cut(s)	308
BsuI	GTATCC	2 cut(s)	130, 735
BsuRI	GGCC	2 cut(s)	804, 968
BsuTUI	ATCGAT	1 cut(s)	308
BtsCI	GGATG	1 cut(s)	574
BtsIMutI	CAGTG	1 cut(s)	1413
BtuMI	TCGCGA	1 cut(s)	1259
Cac8I	GCNNGC	3 cut(s)	802, 1138, 1368
Cfr10I	RCCGGY	1 cut(s)	1366
Cfr13I	GGNCC	4 cut(s)	484, 494, 923, 992
ClaI	ATCGAT	1 cut(s)	308
Csp6I	GTAC	5 cut(s)	220, 249, 353, 905, 1397
CviAII	CATG	7 cut(s)	182, 217, 592, 771, 989, 1072, 1393
CviQI	GTAC	5 cut(s)	220, 249, 353, 905, 1397
DdeI	CTNAG	4 cut(s)	104, 314, 873, 1371
DpnI	GATC	1 cut(s)	675
DpnII	GATC	1 cut(s)	673
DraI	TTTAAA	1 cut(s)	1335
EaeI	YGGCCR	1 cut(s)	802
Eam1104I	CTCTTC	1 cut(s)	434
EarI	CTCTTC	1 cut(s)	434
Eco130I	CCWWGG	2 cut(s)	480, 1065
Eco24I	GRGCYC	1 cut(s)	560
Eco47I	GGWCC	4 cut(s)	484, 494, 923, 992
Eco57I	CTGAAG	1 cut(s)	879
Eco88I	CYCGRG	1 cut(s)	1013
EcoRI	GAATTC	1 cut(s)	960
EcoRII	CCWGG	1 cut(s)	692
EcoT14I	CCWWGG	2 cut(s)	480, 1065
EcoT38I	GRGCYC	1 cut(s)	560
ErhI	CCWWGG	2 cut(s)	480, 1065
Esp3I	CGTCTC	1 cut(s)	1411
FaeI	CATG	7 cut(s)	185, 220, 595, 774, 992, 1075, 1396
FalI	AAGNNNNNCTT	4 cut(s)	1094, 1126, 1268, 1300
FaqI	GGGAC	4 cut(s)	350, 710, 909, 1107
FatI	CATG	7 cut(s)	181, 216, 591, 770, 988, 1071, 1392
FblI	GTMKAC	1 cut(s)	679
FokI	GGATG	1 cut(s)	561
FriOI	GRGCYC	1 cut(s)	560
FspBI	CTAG	4 cut(s)	264, 428, 1119, 1143
GsuI	CTGGAG	1 cut(s)	99
HaeIII	GGCC	2 cut(s)	804, 968
HapII	CCGG	2 cut(s)	1178, 1367
Hin1II	CATG	7 cut(s)	185, 220, 595, 774, 992, 1075, 1396
HincII	GTYRAC	2 cut(s)	39, 244
HindII	GTYRAC	2 cut(s)	39, 244
HindIII	AAGCTT	4 cut(s)	10, 812, 1058, 1433
HinfI	GANTC	5 cut(s)	4, 310, 421, 997, 1387
HpaI	GTTAAC	1 cut(s)	39
HpaII	CCGG	2 cut(s)	1178, 1367
HphI	GGTGA	3 cut(s)	199, 395, 416
Hpy166II	GTNNAC	6 cut(s)	39, 244, 628, 637, 680, 1051
Hpy188I	TCNGA	5 cut(s)	94, 317, 346, 859, 1344
Hpy188III	TCNNGA	7 cut(s)	149, 448, 578, 776, 912, 1258, 1276
Hpy8I	GTNNAC	6 cut(s)	39, 244, 628, 637, 680, 1051
Hpy99I	CGWCG	2 cut(s)	960, 1259
HpyAV	CCTTC	7 cut(s)	219, 461, 515, 854, 918, 979, 1018
HpyCH4III	ACNGT	2 cut(s)	248, 671
HpyCH4V	TGCA	4 cut(s)	26, 843, 1166, 1321
HpyF10VI	GCNNNNNNNGC	5 cut(s)	18, 974, 1107, 1163, 1372
HpyF3I	CTNAG	4 cut(s)	104, 314, 873, 1371
Hsp92II	CATG	7 cut(s)	185, 220, 595, 774, 992, 1075, 1396
KpnI	GGTACC	1 cut(s)	252
KroI	GCCGGC	1 cut(s)	1366
KroNI	GCCGGC	1 cut(s)	1368
KspAI	GTTAAC	1 cut(s)	39
Kzo9I	GATC	1 cut(s)	673
LmnI	GCTCC	7 cut(s)	218, 234, 456, 555, 741, 949, 1230
LweI	GCATC	2 cut(s)	314, 1308
MaeI	CTAG	4 cut(s)	264, 428, 1119, 1143
MaeIII	GTNAC	2 cut(s)	422, 773
MalI	GATC	1 cut(s)	675
MboI	GATC	1 cut(s)	673
MboII	GAAGA	7 cut(s)	47, 55, 67, 271, 293, 296, 451
MhlI	GDGCHC	3 cut(s)	560, 787, 1118
MlsI	TGGCCA	1 cut(s)	804
MluNI	TGGCCA	1 cut(s)	804
MlyI	GAGTC	1 cut(s)	430
MmeI	TCCRAC	1 cut(s)	892
Mox20I	TGGCCA	1 cut(s)	804
MroNI	GCCGGC	1 cut(s)	1366
MroXI	GAANNNNTTC	2 cut(s)	443, 815
MscI	TGGCCA	1 cut(s)	804
MseI	TTAA	7 cut(s)	38, 1088, 1184, 1188, 1334, 1425, 1431
MslI	CAYNNNNRTG	2 cut(s)	579, 833
Msp20I	TGGCCA	1 cut(s)	804
MspA1I	CMGCKG	1 cut(s)	800
MspI	CCGG	2 cut(s)	1178, 1367
MspR9I	CCNGG	1 cut(s)	694
MvaI	CCWGG	1 cut(s)	694
MvnI	CGCG	1 cut(s)	1259
MwoI	GCNNNNNNNGC	5 cut(s)	18, 974, 1107, 1163, 1372
NaeI	GCCGGC	1 cut(s)	1368
NdeII	GATC	1 cut(s)	673
NgoMIV	GCCGGC	1 cut(s)	1366
NlaIII	CATG	7 cut(s)	185, 220, 595, 774, 992, 1075, 1396
NlaIV	GGNNCC	8 cut(s)	250, 557, 751, 786, 866, 925, 945, 1232
NmuCI	GTSAC	2 cut(s)	422, 773
NruI	TCGCGA	1 cut(s)	1259
NspI	RCATGY	2 cut(s)	185, 595
PacI	TTAATTAA	1 cut(s)	1188
PaeR7I	CTCGAG	1 cut(s)	1013
PciI	ACATGT	2 cut(s)	181, 591
PcsI	WCGNNNNNNNCGW	1 cut(s)	13
PdiI	GCCGGC	1 cut(s)	1368
PdmI	GAANNNNTTC	2 cut(s)	443, 815
PfeI	GAWTC	3 cut(s)	310, 997, 1387
PflMI	CCANNNNNTGG	1 cut(s)	763
PleI	GAGTC	1 cut(s)	429
PpsI	GAGTC	1 cut(s)	429
PscI	ACATGT	2 cut(s)	181, 591
PshBI	ATTAAT	1 cut(s)	1088
Psp6I	CCWGG	1 cut(s)	692
PspGI	CCWGG	1 cut(s)	692
PspN4I	GGNNCC	8 cut(s)	250, 557, 751, 786, 866, 925, 945, 1232
PspPI	GGNCC	4 cut(s)	484, 494, 923, 992
PspXI	VCTCGAGB	1 cut(s)	1013
PvuII	CAGCTG	1 cut(s)	800
RruI	TCGCGA	1 cut(s)	1259
RsaI	GTAC	5 cut(s)	221, 250, 354, 906, 1398
RsaNI	GTAC	5 cut(s)	220, 249, 353, 905, 1397
RseI	CAYNNNNRTG	2 cut(s)	579, 833
SaqAI	TTAA	7 cut(s)	38, 1088, 1184, 1188, 1334, 1425, 1431
Sau3AI	GATC	1 cut(s)	673
Sau96I	GGNCC	4 cut(s)	484, 494, 923, 992
SchI	GAGTC	1 cut(s)	430
ScrFI	CCNGG	1 cut(s)	694
SduI	GDGCHC	3 cut(s)	560, 787, 1118
SfaNI	GCATC	2 cut(s)	314, 1308
Sfr274I	CTCGAG	1 cut(s)	1013
SinI	GGWCC	4 cut(s)	484, 494, 923, 992
SlaI	CTCGAG	1 cut(s)	1013
SmiMI	CAYNNNNRTG	2 cut(s)	579, 833
SmlI	CTYRAG	1 cut(s)	1013
SmoI	CTYRAG	1 cut(s)	1013
SpeI	ACTAGT	1 cut(s)	263
SspI	AATATT	1 cut(s)	900
SspMI	CTAG	4 cut(s)	264, 428, 1119, 1143
StyD4I	CCNGG	1 cut(s)	692
StyI	CCWWGG	2 cut(s)	480, 1065
TaaI	ACNGT	2 cut(s)	248, 671
TaqI	TCGA	7 cut(s)	7, 176, 308, 809, 958, 1000, 1014
TaqII	GACCGA	2 cut(s)	911, 1009
TatI	WGTACW	1 cut(s)	352
TfiI	GAWTC	3 cut(s)	310, 997, 1387
Tru1I	TTAA	7 cut(s)	38, 1088, 1184, 1188, 1334, 1425, 1431
Tru9I	TTAA	7 cut(s)	38, 1088, 1184, 1188, 1334, 1425, 1431
TscAI	CASTG	1 cut(s)	1413
TseFI	GTSAC	2 cut(s)	422, 773
Tsp45I	GTSAC	2 cut(s)	422, 773
TspDTI	ATGAA	9 cut(s)	452, 515, 520, 552, 759, 817, 977, 1088, 1391
TspRI	CASTG	1 cut(s)	1413
Van91I	CCANNNNNTGG	1 cut(s)	763
VpaK11BI	GGWCC	4 cut(s)	484, 494, 923, 992
VspI	ATTAAT	1 cut(s)	1088
XapI	RAATTY	3 cut(s)	960, 1041, 1248
XceI	RCATGY	2 cut(s)	185, 595
XhoI	CTCGAG	1 cut(s)	1013
XmiI	GTMKAC	1 cut(s)	679
XmnI	GAANNNNTTC	2 cut(s)	443, 815
XspI	CTAG	4 cut(s)	264, 428, 1119, 1143

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.