pycom04g21420

Glycosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0000987
Physical Location & Seq
Forward (+)
22592547 .. 22595697
3151 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g21420.2

Sequence Viewer

Length: 1431 bp
ATGGCAGCCTTTGAATTCCGGTCACCCTACTTTCTCTCTATAACTTTTCTCCTCCTCCTCCTCCTCCTCCTCCTCTCCTTCTCCCCCTTCAACCAATTCCATTTCACCCCCTTAAATCCTTATTCTTCCCGATCTGCCCCTCTCCAAATCAGAAATTACACAACCTCATATCAATATGCCACTCCGAATCCTGCACCCTCTCCCTCTCCCTCTCCTTCCACTGTTGCAGACAATCTCCAACAGGAAAAGAGCAGCAGCAGCAGCATAACAAGCAAGATTGAAGAGGATTTGGCGAGAGCCAGAGCCGCCATCCGCGAAGCCATTCGTACCAAAAACTACACATCTGACAGACAAGAAATTTACATCCCCAGAGGAAGTATCTACAGAAATCCCTATGCTTTTCACCAGAGTCACATAGAGATGGTGAAAACATTCAAAATATGGGCCTACAAGGAAGGGGAGATACCCATGTTTCACAATGGGCCTATGTCCTACATCTACTCTATTGAAGGCCATTTCATCGATGAGCTGGACAGTAGTGGTAAAAGCCCATTTTTGGCCCGACATCACGACGAGGCCCATTCCTTCTTTGTCCCTGTGAGTGTGAAGAGGATAGCTGATTTCTTGTACGACCGGCCTAATCCCTACACATTTCACGGTCGACTGGTTCGAATCGTCACCGATTACATCAATGTGGTTGCCCAGAAATACCCCTACTGGAATAGGAGCAGTGGAGCTGACCATTTCATGCTCTCTTGTCATGATTGGGCACCGGAAATCATAGACGACGACCGTGACTTCTACAAGAATTTTATAAGAGTACTATGCAATTCCAATACCTCAGAAGGGTTCCAGCCCAAGAGAGACGTCTCATTACCGGAATACAATATACCGGAGCACACTCTTGGCCCATCGCTCCTCGACCAACACCCCGATAAGCGTCCTATCCTAGCCTTCTTCGCCGGTGGTGCTCATGGAGACATAAGGAAGTTCTTGTTTGAGCATTGGAGGGACAAAGATGATGAAATCCAAGTCCATGAGTACCTTCCAAAGGGCCAAAATTACCACGAGATTATGGGACGGACCAAGTTTTGCTTGTGTCCTAGTGGCTCCGAAGTTGCTAGCCCTAGAGTGGTTGAGGCCATGTATGCAGGGTGTGTCCCTGTCCTCATTTCCGATTACTATTCATTGCCCTTCGCTGATGTTCTTGATTGGAGCAAGTTTACGATCGAAATCCCGCCCAAGAGAATACCGGAGATTAAGACGATCCTGAAAGCCGTTCCACACTCAGAGTTCTTGAAGTTGCAGAAGAGGGTGATGCAGGTTAGAAGGCATTTTATGCTGAACCGACCGGCGAAGCCGTATGATGTGTTTCATATGGTGCTTCACTCAATATGGCTAAGGAGGCTTAACATCAGGCTACCAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exostosin

Protein Analysis

477

Amino Acids

55.31

Weight (kDa)

8.57

Isoelectric Point (pI)

57.88

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 815
AatII GACGTC 1 cut(s) 870
Acc36I ACCTGC 1 cut(s) 1312
AccB1I GGYRCC 1 cut(s) 769
AccI GTMKAC 1 cut(s) 661
AccII CGCG 1 cut(s) 315
AciI CCGC 3 cut(s) 306, 313, 1238
AclWI GGATC 1 cut(s) 1261
AcsI RAATTY 3 cut(s) 14, 357, 808
AcyI GRCGYC 1 cut(s) 867
AfaI GTAC 4 cut(s) 328, 629, 822, 1043
AfiI CCNNNNNNNGG 4 cut(s) 556, 846, 1051, 1132
AgsI TTSAA 6 cut(s) 14, 91, 281, 436, 509, 1300
AluBI AGCT 3 cut(s) 529, 617, 737
AluI AGCT 3 cut(s) 529, 617, 737
Alw21I GWGCWC 2 cut(s) 900, 973
Alw26I GTCTC 3 cut(s) 858, 874, 972
AlwI GGATC 1 cut(s) 1261
AoxI GGCC 9 cut(s) 444, 482, 511, 558, 576, 635, 907, 1054, 1140
ApeKI GCWGC 5 cut(s) 5, 252, 255, 258, 261
ApoI RAATTY 3 cut(s) 14, 357, 808
Asp700I GAANNNNTTC 2 cut(s) 321, 431
AspS9I GGNCC 7 cut(s) 444, 482, 559, 577, 908, 1054, 1083
AsuHPI GGTGA 6 cut(s) 15, 97, 395, 436, 670, 1327
AsuII TTCGAA 1 cut(s) 670
AsuNHI GCTAGC 1 cut(s) 1121
AvaII GGWCC 1 cut(s) 1083
BaeGI GKGCMC 1 cut(s) 772
BaeI ACNNNNGTAYC 2 cut(s) 455, 488
BanI GGYRCC 1 cut(s) 769
BarI GAAGNNNNNNTAC 2 cut(s) 447, 479
BauI CACGAG 1 cut(s) 1067
Bbv12I GWGCWC 2 cut(s) 900, 973
BbvI GCAGC 5 cut(s) 17, 264, 267, 270, 273
BccI CCATC 3 cut(s) 317, 415, 919
BceAI ACGGC 2 cut(s) 1262, 1345
BcoDI GTCTC 3 cut(s) 858, 874, 972
BfaI CTAG 4 cut(s) 950, 1104, 1122, 1128
BfmI CTRYAG 1 cut(s) 382
BfuAI ACCTGC 1 cut(s) 1312
BisI GCNGC 6 cut(s) 6, 253, 256, 259, 262, 306
BlsI GCNGC 6 cut(s) 7, 254, 257, 260, 263, 307
BmcAI AGTACT 1 cut(s) 822
Bme18I GGWCC 1 cut(s) 1083
BmgT120I GGNCC 7 cut(s) 444, 482, 559, 577, 908, 1054, 1083
BmiI GGNNCC 3 cut(s) 771, 851, 1111
BmsI GCATC 1 cut(s) 1308
BmtI GCTAGC 1 cut(s) 1125
Bpu10I CCTNAGC 1 cut(s) 1400
Bpu14I TTCGAA 1 cut(s) 670
Bsa29I ATCGAT 1 cut(s) 522
BsaBI GATNNNNATC 1 cut(s) 1232
BsaHI GRCGYC 1 cut(s) 867
BsaWI WCCGGW 5 cut(s) 18, 772, 877, 892, 1252
Bsc4I CCNNNNNNNGG 4 cut(s) 556, 846, 1051, 1132
Bse118I RCCGGY 3 cut(s) 633, 962, 1351
Bse1I ACTGG 2 cut(s) 669, 722
Bse3DI GCAATG 1 cut(s) 1187
Bse8I GATNNNNATC 1 cut(s) 1232
BseCI ATCGAT 1 cut(s) 522
BseGI GGATG 2 cut(s) 309, 363
BseJI GATNNNNATC 1 cut(s) 1232
BseLI CCNNNNNNNGG 4 cut(s) 556, 846, 1051, 1132
BseMI GCAATG 1 cut(s) 1187
BseMII CTCAG 2 cut(s) 855, 1302
BseNI ACTGG 2 cut(s) 669, 722
BseRI GAGGAG 9 cut(s) 41, 44, 47, 50, 53, 56, 59, 62, 908
BseSI GKGCMC 1 cut(s) 772
BseXI GCAGC 5 cut(s) 17, 264, 267, 270, 273
BsgI GTGCAG 1 cut(s) 177
Bsh1236I CGCG 1 cut(s) 315
Bsh1285I CGRYCG 5 cut(s) 634, 661, 793, 1230, 1352
BshFI GGCC 9 cut(s) 446, 484, 513, 560, 578, 637, 909, 1056, 1142
BshNI GGYRCC 1 cut(s) 769
BshVI ATCGAT 1 cut(s) 522
BsiEI CGRYCG 5 cut(s) 634, 661, 793, 1230, 1352
BsiHKAI GWGCWC 2 cut(s) 900, 973
BsiSI CCGG 8 cut(s) 19, 634, 773, 878, 893, 963, 1253, 1352
BslFI GGGAC 4 cut(s) 578, 1025, 1092, 1145
BslI CCNNNNNNNGG 4 cut(s) 556, 846, 1051, 1132
BsmAI GTCTC 3 cut(s) 858, 874, 972
BsmBI CGTCTC 2 cut(s) 858, 874
BsmFI GGGAC 4 cut(s) 578, 1025, 1092, 1145
BsnI GGCC 9 cut(s) 446, 484, 513, 560, 578, 637, 909, 1056, 1142
Bsp119I TTCGAA 1 cut(s) 670
Bsp1286I GDGCHC 3 cut(s) 772, 900, 973
Bsp143I GATC 3 cut(s) 131, 1227, 1266
BspACI CCGC 3 cut(s) 306, 313, 1238
BspANI GGCC 9 cut(s) 446, 484, 513, 560, 578, 637, 909, 1056, 1142
BspCNI CTCAG 2 cut(s) 854, 1301
BspDI ATCGAT 1 cut(s) 522
BspFNI CGCG 1 cut(s) 315
BspHI TCATGA 1 cut(s) 760
BspLI GGNNCC 3 cut(s) 771, 851, 1111
BspMI ACCTGC 1 cut(s) 1312
BspOI GCTAGC 1 cut(s) 1125
BspPI GGATC 1 cut(s) 1261
BspT104I TTCGAA 1 cut(s) 670
BspT107I GGYRCC 1 cut(s) 769
BsrDI GCAATG 1 cut(s) 1187
BsrFI RCCGGY 3 cut(s) 633, 962, 1351
BsrI ACTGG 2 cut(s) 669, 722
BssAI RCCGGY 3 cut(s) 633, 962, 1351
BssMI GATC 3 cut(s) 131, 1227, 1266
BssNI GRCGYC 1 cut(s) 867
BssSI CACGAG 1 cut(s) 1067
Bst2BI CACGAG 1 cut(s) 1067
Bst4CI ACNGT 4 cut(s) 223, 536, 659, 794
Bst6I CTCTTC 3 cut(s) 276, 602, 1304
BstACI GRCGYC 1 cut(s) 867
BstAPI GCANNNNNTGC 1 cut(s) 1339
BstBI TTCGAA 1 cut(s) 670
BstC8I GCNNGC 1 cut(s) 1123
BstDEI CTNAG 3 cut(s) 841, 1288, 1400
BstEII GGTNACC 1 cut(s) 21
BstENI CCTNNNNNAGG 1 cut(s) 1049
BstF5I GGATG 2 cut(s) 309, 363
BstFNI CGCG 1 cut(s) 315
BstKTI GATC 3 cut(s) 134, 1230, 1269
BstMAI GTCTC 3 cut(s) 858, 874, 972
BstMBI GATC 3 cut(s) 131, 1227, 1266
BstMCI CGRYCG 5 cut(s) 634, 661, 793, 1230, 1352
BstMWI GCNNNNNNNGC 9 cut(s) 258, 261, 270, 305, 959, 968, 1148, 1339, 1405
BstPI GGTNACC 1 cut(s) 21
BstSFI CTRYAG 1 cut(s) 382
BstSLI GKGCMC 1 cut(s) 772
BstUI CGCG 1 cut(s) 315
BstV1I GCAGC 5 cut(s) 17, 264, 267, 270, 273
Bsu15I ATCGAT 1 cut(s) 522
BsuRI GGCC 9 cut(s) 446, 484, 513, 560, 578, 637, 909, 1056, 1142
BsuTUI ATCGAT 1 cut(s) 522
BtgZI GCGATG 1 cut(s) 897
BtsCI GGATG 2 cut(s) 309, 363
BtsI GCAGTG 1 cut(s) 736
BtsIMutI CAGTG 2 cut(s) 219, 736
BveI ACCTGC 1 cut(s) 1312
Cac8I GCNNGC 1 cut(s) 1123
CciI TCATGA 1 cut(s) 760
Cfr10I RCCGGY 3 cut(s) 633, 962, 1351
Cfr13I GGNCC 7 cut(s) 444, 482, 559, 577, 908, 1054, 1083
ClaI ATCGAT 1 cut(s) 522
CseI GACGC 1 cut(s) 929
Csp6I GTAC 4 cut(s) 327, 628, 821, 1042
CviAII CATG 6 cut(s) 469, 748, 761, 974, 1037, 1144
CviQI GTAC 4 cut(s) 327, 628, 821, 1042
DdeI CTNAG 3 cut(s) 841, 1288, 1400
DpnI GATC 3 cut(s) 133, 1229, 1268
DpnII GATC 3 cut(s) 131, 1227, 1266
Eam1104I CTCTTC 3 cut(s) 276, 602, 1304
EarI CTCTTC 3 cut(s) 276, 602, 1304
Eco47I GGWCC 1 cut(s) 1083
Eco91I GGTNACC 1 cut(s) 21
EcoNI CCTNNNNNAGG 1 cut(s) 1049
EcoO65I GGTNACC 1 cut(s) 21
EcoRI GAATTC 1 cut(s) 14
Esp3I CGTCTC 2 cut(s) 858, 874
FaeI CATG 6 cut(s) 472, 751, 764, 977, 1040, 1147
FalI AAGNNNNNCTT 2 cut(s) 1079, 1111
FaqI GGGAC 4 cut(s) 578, 1025, 1092, 1145
FatI CATG 6 cut(s) 468, 747, 760, 973, 1036, 1143
FauI CCCGC 1 cut(s) 1245
FauNDI CATATG 1 cut(s) 1377
FblI GTMKAC 1 cut(s) 661
Fnu4HI GCNGC 6 cut(s) 6, 253, 256, 259, 262, 306
FokI GGATG 2 cut(s) 296, 350
Fsp4HI GCNGC 6 cut(s) 6, 253, 256, 259, 262, 306
FspBI CTAG 4 cut(s) 950, 1104, 1122, 1128
GluI GCNGC 6 cut(s) 6, 253, 256, 259, 262, 306
HaeIII GGCC 9 cut(s) 446, 484, 513, 560, 578, 637, 909, 1056, 1142
HapII CCGG 8 cut(s) 19, 634, 773, 878, 893, 963, 1253, 1352
HgaI GACGC 1 cut(s) 929
Hin1I GRCGYC 1 cut(s) 867
Hin1II CATG 6 cut(s) 472, 751, 764, 977, 1040, 1147
HincII GTYRAC 1 cut(s) 662
HindII GTYRAC 1 cut(s) 662
HinfI GANTC 3 cut(s) 187, 409, 672
HpaII CCGG 8 cut(s) 19, 634, 773, 878, 893, 963, 1253, 1352
HphI GGTGA 6 cut(s) 15, 97, 395, 436, 670, 1327
Hpy166II GTNNAC 2 cut(s) 662, 1224
Hpy188I TCNGA 7 cut(s) 152, 186, 346, 844, 1114, 1177, 1291
Hpy188III TCNNGA 6 cut(s) 129, 569, 761, 1208, 1270, 1297
Hpy8I GTNNAC 2 cut(s) 662, 1224
Hpy99I CGWCG 2 cut(s) 575, 791
HpyCH4III ACNGT 4 cut(s) 223, 536, 659, 794
HpyCH4IV ACGT 1 cut(s) 867
HpyCH4V TGCA 6 cut(s) 194, 227, 828, 1151, 1306, 1321
HpyF10VI GCNNNNNNNGC 9 cut(s) 258, 261, 270, 305, 959, 968, 1148, 1339, 1405
HpyF3I CTNAG 3 cut(s) 841, 1288, 1400
HpySE526I ACGT 1 cut(s) 867
Hsp92I GRCGYC 1 cut(s) 867
Hsp92II CATG 6 cut(s) 472, 751, 764, 977, 1040, 1147
Kzo9I GATC 3 cut(s) 131, 1227, 1266
LmnI GCTCC 6 cut(s) 726, 734, 895, 921, 1115, 1215
Lsp1109I GCAGC 5 cut(s) 17, 264, 267, 270, 273
LweI GCATC 1 cut(s) 1308
MaeI CTAG 4 cut(s) 950, 1104, 1122, 1128
MaeII ACGT 1 cut(s) 867
MaeIII GTNAC 4 cut(s) 21, 410, 676, 794
MalI GATC 3 cut(s) 133, 1229, 1268
MboI GATC 3 cut(s) 131, 1227, 1266
MboII GAAGA 5 cut(s) 117, 293, 619, 949, 1321
MhlI GDGCHC 3 cut(s) 772, 900, 973
MluCI AATT 8 cut(s) 14, 95, 154, 357, 808, 829, 1060, 1426
MlyI GAGTC 1 cut(s) 418
MmeI TCCRAC 1 cut(s) 262
MroXI GAANNNNTTC 2 cut(s) 321, 431
MseI TTAA 3 cut(s) 113, 1260, 1410
MslI CAYNNNNRTG 2 cut(s) 419, 692
MspI CCGG 8 cut(s) 19, 634, 773, 878, 893, 963, 1253, 1352
MvnI CGCG 1 cut(s) 315
MwoI GCNNNNNNNGC 9 cut(s) 258, 261, 270, 305, 959, 968, 1148, 1339, 1405
NdeI CATATG 1 cut(s) 1377
NdeII GATC 3 cut(s) 131, 1227, 1266
NheI GCTAGC 1 cut(s) 1121
NlaIII CATG 6 cut(s) 472, 751, 764, 977, 1040, 1147
NlaIV GGNNCC 3 cut(s) 771, 851, 1111
NmuCI GTSAC 4 cut(s) 21, 410, 676, 794
NspV TTCGAA 1 cut(s) 670
PagI TCATGA 1 cut(s) 760
PcsI WCGNNNNNNNCGW 1 cut(s) 667
PdmI GAANNNNTTC 2 cut(s) 321, 431
PfeI GAWTC 2 cut(s) 187, 672
PkrI GCNGC 6 cut(s) 7, 254, 257, 260, 263, 307
Ple19I CGATCG 1 cut(s) 1230
PleI GAGTC 1 cut(s) 417
PpsI GAGTC 1 cut(s) 417
PsiI TTATAA 1 cut(s) 815
PspEI GGTNACC 1 cut(s) 21
PspN4I GGNNCC 3 cut(s) 771, 851, 1111
PspPI GGNCC 7 cut(s) 444, 482, 559, 577, 908, 1054, 1083
PvuI CGATCG 1 cut(s) 1230
RsaI GTAC 4 cut(s) 328, 629, 822, 1043
RsaNI GTAC 4 cut(s) 327, 628, 821, 1042
RseI CAYNNNNRTG 2 cut(s) 419, 692
SalI GTCGAC 1 cut(s) 660
SaqAI TTAA 3 cut(s) 113, 1260, 1410
SatI GCNGC 6 cut(s) 6, 253, 256, 259, 262, 306
Sau3AI GATC 3 cut(s) 131, 1227, 1266
Sau96I GGNCC 7 cut(s) 444, 482, 559, 577, 908, 1054, 1083
ScaI AGTACT 1 cut(s) 822
SchI GAGTC 1 cut(s) 418
SduI GDGCHC 3 cut(s) 772, 900, 973
SetI ASST 8 cut(s) 167, 531, 619, 739, 842, 870, 1047, 1326
SfaNI GCATC 1 cut(s) 1308
SfcI CTRYAG 1 cut(s) 382
SfuI TTCGAA 1 cut(s) 670
SgrAI CRCCGGYG 1 cut(s) 962
SinI GGWCC 1 cut(s) 1083
SmiMI CAYNNNNRTG 2 cut(s) 419, 692
Sse9I AATT 8 cut(s) 14, 95, 154, 357, 808, 829, 1060, 1426
SsiI CCGC 3 cut(s) 306, 313, 1238
SspMI CTAG 4 cut(s) 950, 1104, 1122, 1128
TaaI ACNGT 4 cut(s) 223, 536, 659, 794
TaiI ACGT 1 cut(s) 870
TaqI TCGA 5 cut(s) 522, 661, 670, 921, 1230
TasI AATT 8 cut(s) 14, 95, 154, 357, 808, 829, 1060, 1426
TatI WGTACW 1 cut(s) 820
TauI GCSGC 1 cut(s) 308
TfiI GAWTC 2 cut(s) 187, 672
Tru1I TTAA 3 cut(s) 113, 1260, 1410
Tru9I TTAA 3 cut(s) 113, 1260, 1410
TscAI CASTG 2 cut(s) 226, 736
TseFI GTSAC 4 cut(s) 21, 410, 676, 794
TseI GCWGC 5 cut(s) 5, 252, 255, 258, 261
Tsp45I GTSAC 4 cut(s) 21, 410, 676, 794
TspDTI ATGAA 5 cut(s) 508, 736, 1038, 1176, 1364
TspGWI ACGGA 1 cut(s) 1096
TspRI CASTG 2 cut(s) 226, 736
VpaK11BI GGWCC 1 cut(s) 1083
XagI CCTNNNNNAGG 1 cut(s) 1049
XapI RAATTY 3 cut(s) 14, 357, 808
XmiI GTMKAC 1 cut(s) 661
XmnI GAANNNNTTC 2 cut(s) 321, 431
XspI CTAG 4 cut(s) 950, 1104, 1122, 1128
ZraI GACGTC 1 cut(s) 868
ZrmI AGTACT 1 cut(s) 822
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.