Prupe.4G177700_v2.0.a1

MATE efflux family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0000715
Physical Location & Seq
Forward (+)
10533039 .. 10537259
4221 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G177700.1

Sequence Viewer

Length: 1464 bp
ATGTCAGCAAACACAGTTGCAGAATCTGCAGACAGGAGCACTGCTACACCGTTATTAGAGGCAAGCGGCCATGGCGATCAACAAGGAAGAAAAGGGGGGTGGTGGAATAAACTGTTGGACATGGAGGAGGCCAAGAACCAGGTCTTGTTTTCCTTGCCAATGATTCTTACCAATGTTTGCTATTACATGATCCCCTTGATATCTGTCATGCTTGCTGGTCACCTTGGTGAGCTTGAGCTTGCTGGTGCCACACTTGCCAATTCATGGGCCACCGTTACCGGTTTCGCTCTCATGATTGGGTTAAGTGGAGCTCTTGAGACACTGTGTGGGCAAGGATTTGGAGCAAAGTTATACAGAATGTTGGGGATATATCTACAAGCCTCATCCATAATTTCTTTCTTGGTCTCTATCATCATATCTGTAGTCTGGTTCTACACAGAACCCATACTTATCTTACTTCATCAAGATCCTCAAATTTCAAAATCTGCTGCTCTTTATATGAAATTTCTGATTCCGGGATTATTTGCATTCGGCTTCATGCATAACATATTGAGATTTCTTCAGACCCAATCCGTTGTAATGCCGTTGATCTTCTTCTCACTGATCCCAGTGGTTATTCAGATTTTCGTTGCATATGCTTTGGTACATTGGACAGCTCTTGGTTTCAAGGGAGCTCCCCTAGCATGTTCAATTTCGATATGGATAGCGATGCTAATGCTGGCCATGTATGTTATGTTTGCAAAGAAGTTTGAGCTTACATGGGAAGGATTTTCGATAGAATCGTTCCATTATGTCCTTGCTGCCTTGAAACTTGCCCTCCCCTCAGCAGCAATGGTATGTTTGGAGTACTGGGCTTTTGAGATTCTGGTGTTATTGGCAGGACTGATGCCAAACGCAGAACAAACCACTTCACTAATTGCAATGTGTGTGAATACAGAAGCAATTGCATACATGATTACATATGGCCTCAGTGCTGCTGCAAGCACAAGGGTGTCAAATGAATTAGGAGCAGGCAATCCTGACAACGCCAAGAAGGCAATGGCTGTGACTCTTAAGCTCTCTGTGCTTCTTGGTCTCCTTGTTGTTCTGGCTCTAGCATTTGGTCACAATATATGGGCTGGCTTTTTCAGTGACAGCAGTGCAATAATCAAGCTTTTTGCTTCTATGACACCATTTCTTGCAATTTCAATAATGGCTGATTCTGTGCAAGGTGTCTTGTCAGGTGTGGCCAGAGGATGTGGCTTTCAGCACTTGGCTATGTATGTAAACTTGGGTACATTCTATTTGGTTGGCATGACAGTTGCATGTGTTGTTGGATTTAAGTTGAAACTATATGCTAAGGGATTGTGGATTGGCTTAATCTGTGGTCTTTGCTGTCAAGCCGCCCTACTTTTGCTGGTTACACTGCGGACAAAATGGACTCAATTGGATGTGCCTCATAATGAAGATGCAGTTTTGGTCTAA

Protein Analysis

488

Amino Acids

52.8

Weight (kDa)

6.74

Isoelectric Point (pI)

33.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000715)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23550 AT3G23560 AT3G23560
fragaria_vesca FvH4_1g15630 FvH4_1g15630 FvH4_3g19700 FvH4_3g19700 FvH4_3g19700 FvH4_3g19700 FvH4_3g19700 FvH4_3g19720 FvH4_3g20060 FvH4_3g20060 FvH4_3g20060 FvH4_3g20060 FvH4_5g31740 FvH4_7g19991
malus_domestica MD03G1230300.v1.1 MD11G1250700.v1.1 MD11G1251300.v1.1 MD11G1251400.v1.1 MD11G1251500.v1.1 MD12G1088700.v1.1
prunus_persica Prupe.4G177700_v2.0.a1 Prupe.4G177700_v2.0.a1 Prupe.4G177900_v2.0.a1 Prupe.4G178700_v2.0.a1 Prupe.4G178700_v2.0.a1 Prupe.4G178700_v2.0.a1 Prupe.4G178700_v2.0.a1 Prupe.4G178700_v2.0.a1 Prupe.4G178700_v2.0.a1 Prupe.4G178700_v2.0.a1 Prupe.4G178700_v2.0.a1 Prupe.4G178700_v2.0.a1 Prupe.4G178700_v2.0.a1 Prupe.4G178700_v2.0.a1 Prupe.4G178700_v2.0.a1 Prupe.4G178900_v2.0.a1 Prupe.4G178900_v2.0.a1 Prupe.4G178900_v2.0.a1 Prupe.4G206700_v2.0.a1
pyrus_communis pycom03g17780 pycom10g06980 pycom11g22170 pycom11g22270
rosa_chinensis RchiOBHm_Chr5g0033011 RchiOBHm_Chr5g0033061
rosa_laevigata RLG00000033443 RLG00000033445
rosa_multiflora Rmu_sc0000986.1_g000015
rosa_roxburghii Rroxscaffold_1G00046880 Rroxscaffold_1G00046890 Rroxscaffold_1G00046990 Rroxscaffold_1G00047000 Rroxscaffold_1G00047040
rosa_rugosa Rorug05G0135000 Rorug05G0135100 Rorug05G0135200 Rorug05G0135500 Rorug05G0135600 Rorug05G0135700 Rorug05G0135800
rosa_samantha Rh5AG227700 Rh5AG228200 Rh5BG229300 Rh5BG229400 Rh5CG256700 Rh5CG257200 Rh5DG233900 Rh5DG234100
rosa_wichuraiana Rw5G020860 Rw5G020880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 245
AccB7I CCANNNNNTGG 1 cut(s) 264
AciI CCGC 3 cut(s) 66, 1383, 1408
AclWI GGATC 3 cut(s) 184, 461, 598
AcoI YGGCCR 3 cut(s) 67, 720, 1227
AcsI RAATTY 2 cut(s) 474, 503
AcuI CTGAAG 1 cut(s) 545
AdeI CACNNNGTG 1 cut(s) 326
AfaI GTAC 3 cut(s) 645, 848, 1276
AfiI CCNNNNNNNGG 1 cut(s) 264
AflII CTTAAG 1 cut(s) 1052
AgeI ACCGGT 1 cut(s) 278
AgsI TTSAA 6 cut(s) 480, 667, 690, 808, 1188, 1327
AjnI CCWGG 1 cut(s) 138
AjuI GAANNNNNNNTTGG 2 cut(s) 98, 130
AleI CACNNNNGTG 2 cut(s) 225, 989
AluBI AGCT 8 cut(s) 232, 238, 311, 656, 674, 754, 1057, 1153
AluI AGCT 8 cut(s) 232, 238, 311, 656, 674, 754, 1057, 1153
Alw21I GWGCWC 3 cut(s) 41, 313, 676
Alw26I GTCTC 3 cut(s) 311, 409, 1079
AlwI GGATC 3 cut(s) 184, 461, 598
AlwNI CAGNNNCTG 1 cut(s) 26
AoxI GGCC 6 cut(s) 67, 129, 267, 720, 964, 1227
ApeKI GCWGC 5 cut(s) 488, 800, 827, 974, 977
ApoI RAATTY 2 cut(s) 474, 503
AsiGI ACCGGT 1 cut(s) 278
AspS9I GGNCC 1 cut(s) 267
AsuC2I CCSGG 1 cut(s) 516
AsuHPI GGTGA 2 cut(s) 212, 239
BalI TGGCCA 2 cut(s) 722, 1229
BanI GGYRCC 1 cut(s) 245
BanII GRGCYC 2 cut(s) 313, 676
Bbv12I GWGCWC 3 cut(s) 41, 313, 676
BbvCI CCTCAGC 1 cut(s) 823
BbvI GCAGC 5 cut(s) 475, 787, 839, 961, 964
BceAI ACGGC 1 cut(s) 568
BcgI CGANNNNNNTGC 2 cut(s) 697, 731
BciT130I CCWGG 1 cut(s) 140
BcnI CCSGG 1 cut(s) 516
BcoDI GTCTC 3 cut(s) 311, 409, 1079
BfaI CTAG 2 cut(s) 680, 1094
BfmI CTRYAG 2 cut(s) 27, 420
BfrI CTTAAG 1 cut(s) 1052
BglI GCCNNNNNGGC 1 cut(s) 1034
BisI GCNGC 7 cut(s) 67, 489, 801, 828, 975, 978, 1383
BlsI GCNGC 7 cut(s) 68, 490, 802, 829, 976, 979, 1384
BmcAI AGTACT 1 cut(s) 848
Bme1390I CCNGG 2 cut(s) 140, 516
BmgT120I GGNCC 1 cut(s) 267
BmiI GGNNCC 1 cut(s) 247
BmrFI CCNGG 2 cut(s) 140, 516
BmrI ACTGGG 2 cut(s) 602, 859
BmsI GCATC 3 cut(s) 699, 876, 1438
BmuI ACTGGG 2 cut(s) 602, 859
Bpu10I CCTNAGC 2 cut(s) 823, 1338
BpuEI CTTGAG 2 cut(s) 254, 335
BpuMI CCSGG 1 cut(s) 516
BsaI GGTCTC 2 cut(s) 409, 1079
BsaJI CCNNGG 2 cut(s) 70, 223
BsaWI WCCGGW 1 cut(s) 278
BsaXI ACNNNNNCTCC 2 cut(s) 801, 831
Bsc4I CCNNNNNNNGG 1 cut(s) 264
Bse118I RCCGGY 1 cut(s) 278
Bse1I ACTGG 2 cut(s) 608, 854
Bse3DI GCAATG 3 cut(s) 837, 927, 1044
BseBI CCWGG 1 cut(s) 140
BseDI CCNNGG 2 cut(s) 70, 223
BseGI GGATG 3 cut(s) 383, 1241, 1435
BseLI CCNNNNNNNGG 1 cut(s) 264
BseMI GCAATG 3 cut(s) 837, 927, 1044
BseMII CTCAG 2 cut(s) 837, 982
BseNI ACTGG 2 cut(s) 608, 854
BseRI GAGGAG 1 cut(s) 140
BseXI GCAGC 5 cut(s) 475, 787, 839, 961, 964
BshFI GGCC 6 cut(s) 69, 131, 269, 722, 966, 1229
BshNI GGYRCC 1 cut(s) 245
BshTI ACCGGT 1 cut(s) 278
BsiHKAI GWGCWC 3 cut(s) 41, 313, 676
BsiSI CCGG 2 cut(s) 279, 515
BslI CCNNNNNNNGG 1 cut(s) 264
BsmAI GTCTC 3 cut(s) 311, 409, 1079
BsmI GAATGC 1 cut(s) 527
BsnI GGCC 6 cut(s) 69, 131, 269, 722, 966, 1229
Bso31I GGTCTC 2 cut(s) 409, 1079
Bsp1286I GDGCHC 3 cut(s) 41, 313, 676
Bsp143I GATC 5 cut(s) 76, 189, 466, 588, 603
Bsp19I CCATGG 1 cut(s) 70
BspACI CCGC 3 cut(s) 66, 1383, 1408
BspANI GGCC 6 cut(s) 69, 131, 269, 722, 966, 1229
BspCNI CTCAG 2 cut(s) 836, 981
BspHI TCATGA 1 cut(s) 291
BspLI GGNNCC 1 cut(s) 247
BspMAI CTGCAG 1 cut(s) 31
BspPI GGATC 3 cut(s) 184, 461, 598
BspT107I GGYRCC 1 cut(s) 245
BspTI CTTAAG 1 cut(s) 1052
BspTNI GGTCTC 2 cut(s) 409, 1079
BsrDI GCAATG 3 cut(s) 837, 927, 1044
BsrFI RCCGGY 1 cut(s) 278
BsrI ACTGG 2 cut(s) 608, 854
BssAI RCCGGY 1 cut(s) 278
BssECI CCNNGG 2 cut(s) 70, 223
BssMI GATC 5 cut(s) 76, 189, 466, 588, 603
BssT1I CCWWGG 2 cut(s) 70, 223
Bst2UI CCWGG 1 cut(s) 140
Bst4CI ACNGT 6 cut(s) 16, 51, 114, 274, 324, 1300
BstAFI CTTAAG 1 cut(s) 1052
BstAPI GCANNNNNTGC 1 cut(s) 26
BstC8I GCNNGC 7 cut(s) 64, 213, 240, 720, 982, 1012, 1120
BstDEI CTNAG 3 cut(s) 823, 968, 1338
BstDSI CCRYGG 1 cut(s) 70
BstEII GGTNACC 1 cut(s) 218
BstF5I GGATG 3 cut(s) 383, 1241, 1435
BstKTI GATC 5 cut(s) 79, 192, 469, 591, 606
BstMAI GTCTC 3 cut(s) 311, 409, 1079
BstMBI GATC 5 cut(s) 76, 189, 466, 588, 603
BstMWI GCNNNNNNNGC 6 cut(s) 26, 72, 254, 680, 1034, 1063
BstNI CCWGG 1 cut(s) 140
BstNSI RCATGY 2 cut(s) 687, 1308
BstPI GGTNACC 1 cut(s) 218
BstSCI CCNGG 2 cut(s) 138, 514
BstSFI CTRYAG 2 cut(s) 27, 420
BstV1I GCAGC 5 cut(s) 475, 787, 839, 961, 964
BstX2I RGATCY 1 cut(s) 466
BstYI RGATCY 1 cut(s) 466
BsuRI GGCC 6 cut(s) 69, 131, 269, 722, 966, 1229
BtgI CCRYGG 1 cut(s) 70
BtgZI GCGATG 1 cut(s) 722
BtsCI GGATG 3 cut(s) 383, 1241, 1435
BtsI GCAGTG 3 cut(s) 39, 1144, 1403
BtsIMutI CAGTG 8 cut(s) 39, 320, 599, 615, 976, 1135, 1144, 1403
Cac8I GCNNGC 7 cut(s) 64, 213, 240, 720, 982, 1012, 1120
CaiI CAGNNNCTG 1 cut(s) 26
CciI TCATGA 1 cut(s) 291
Cfr10I RCCGGY 1 cut(s) 278
Cfr13I GGNCC 1 cut(s) 267
CsiI ACCWGGT 1 cut(s) 138
Csp6I GTAC 3 cut(s) 644, 847, 1275
CspAI ACCGGT 1 cut(s) 278
CviQI GTAC 3 cut(s) 644, 847, 1275
DdeI CTNAG 3 cut(s) 823, 968, 1338
DpnI GATC 5 cut(s) 78, 191, 468, 590, 605
DpnII GATC 5 cut(s) 76, 189, 466, 588, 603
DraIII CACNNNGTG 1 cut(s) 326
EaeI YGGCCR 3 cut(s) 67, 720, 1227
Ecl136II GAGCTC 2 cut(s) 311, 674
Eco130I CCWWGG 2 cut(s) 70, 223
Eco24I GRGCYC 2 cut(s) 313, 676
Eco31I GGTCTC 2 cut(s) 409, 1079
Eco32I GATATC 1 cut(s) 201
Eco53kI GAGCTC 2 cut(s) 311, 674
Eco57I CTGAAG 1 cut(s) 545
Eco91I GGTNACC 1 cut(s) 218
EcoICRI GAGCTC 2 cut(s) 311, 674
EcoO65I GGTNACC 1 cut(s) 218
EcoRII CCWGG 1 cut(s) 138
EcoRV GATATC 1 cut(s) 201
EcoT14I CCWWGG 2 cut(s) 70, 223
EcoT22I ATGCAT 1 cut(s) 543
EcoT38I GRGCYC 2 cut(s) 313, 676
ErhI CCWWGG 2 cut(s) 70, 223
FauNDI CATATG 2 cut(s) 634, 961
Fnu4HI GCNGC 7 cut(s) 67, 489, 801, 828, 975, 978, 1383
FokI GGATG 3 cut(s) 370, 1248, 1442
FriOI GRGCYC 2 cut(s) 313, 676
Fsp4HI GCNGC 7 cut(s) 67, 489, 801, 828, 975, 978, 1383
FspBI CTAG 2 cut(s) 680, 1094
GluI GCNGC 7 cut(s) 67, 489, 801, 828, 975, 978, 1383
HaeIII GGCC 6 cut(s) 69, 131, 269, 722, 966, 1229
HapII CCGG 2 cut(s) 279, 515
HindIII AAGCTT 1 cut(s) 1151
HinfI GANTC 8 cut(s) 23, 163, 511, 779, 862, 1048, 1199, 1420
HpaII CCGG 2 cut(s) 279, 515
HphI GGTGA 2 cut(s) 212, 239
Hpy166II GTNNAC 1 cut(s) 1267
Hpy188I TCNGA 3 cut(s) 510, 564, 621
Hpy188III TCNNGA 4 cut(s) 292, 314, 464, 1019
Hpy8I GTNNAC 1 cut(s) 1267
HpyAV CCTTC 2 cut(s) 758, 1027
HpyCH4III ACNGT 6 cut(s) 16, 51, 114, 274, 324, 1300
HpyF10VI GCNNNNNNNGC 6 cut(s) 26, 72, 254, 680, 1034, 1063
HpyF3I CTNAG 3 cut(s) 823, 968, 1338
Kzo9I GATC 5 cut(s) 76, 189, 466, 588, 603
LmnI GCTCC 6 cut(s) 36, 308, 341, 671, 679, 1007
Lsp1109I GCAGC 5 cut(s) 475, 787, 839, 961, 964
LweI GCATC 3 cut(s) 699, 876, 1438
MabI ACCWGGT 1 cut(s) 138
MaeI CTAG 2 cut(s) 680, 1094
MaeIII GTNAC 6 cut(s) 218, 274, 1045, 1103, 1130, 1399
MalI GATC 5 cut(s) 78, 191, 468, 590, 605
MboI GATC 5 cut(s) 76, 189, 466, 588, 603
MboII GAAGA 5 cut(s) 99, 551, 583, 586, 1457
MfeI CAATTG 2 cut(s) 942, 1424
MflI RGATCY 1 cut(s) 466
MhlI GDGCHC 3 cut(s) 41, 313, 676
MlsI TGGCCA 2 cut(s) 722, 1229
MluNI TGGCCA 2 cut(s) 722, 1229
MlyI GAGTC 2 cut(s) 1042, 1414
MmeI TCCRAC 2 cut(s) 96, 1294
Mox20I TGGCCA 2 cut(s) 722, 1229
Mph1103I ATGCAT 1 cut(s) 543
MscI TGGCCA 2 cut(s) 722, 1229
MseI TTAA 4 cut(s) 302, 1053, 1320, 1358
MslI CAYNNNNRTG 2 cut(s) 225, 989
Msp20I TGGCCA 2 cut(s) 722, 1229
MspCI CTTAAG 1 cut(s) 1052
MspI CCGG 2 cut(s) 279, 515
MspR9I CCNGG 2 cut(s) 140, 516
MunI CAATTG 2 cut(s) 942, 1424
Mva1269I GAATGC 1 cut(s) 527
MvaI CCWGG 1 cut(s) 140
MwoI GCNNNNNNNGC 6 cut(s) 26, 72, 254, 680, 1034, 1063
NciI CCSGG 1 cut(s) 516
NcoI CCATGG 1 cut(s) 70
NdeI CATATG 2 cut(s) 634, 961
NdeII GATC 5 cut(s) 76, 189, 466, 588, 603
NlaIV GGNNCC 1 cut(s) 247
NmuCI GTSAC 4 cut(s) 218, 1045, 1103, 1130
NsiI ATGCAT 1 cut(s) 543
NspI RCATGY 2 cut(s) 687, 1308
OliI CACNNNNGTG 2 cut(s) 225, 989
PagI TCATGA 1 cut(s) 291
PcsI WCGNNNNNNNCGW 1 cut(s) 779
PctI GAATGC 1 cut(s) 527
PfeI GAWTC 6 cut(s) 23, 163, 511, 779, 862, 1199
PflMI CCANNNNNTGG 1 cut(s) 264
PfoI TCCNGGA 1 cut(s) 514
PinAI ACCGGT 1 cut(s) 278
PkrI GCNGC 7 cut(s) 68, 490, 802, 829, 976, 979, 1384
PleI GAGTC 2 cut(s) 1042, 1414
PpsI GAGTC 2 cut(s) 1042, 1414
Psp124BI GAGCTC 2 cut(s) 313, 676
Psp6I CCWGG 1 cut(s) 138
PspEI GGTNACC 1 cut(s) 218
PspGI CCWGG 1 cut(s) 138
PspN4I GGNNCC 1 cut(s) 247
PspPI GGNCC 1 cut(s) 267
PstI CTGCAG 1 cut(s) 31
PstNI CAGNNNCTG 1 cut(s) 26
PsuI RGATCY 1 cut(s) 466
RsaI GTAC 3 cut(s) 645, 848, 1276
RsaNI GTAC 3 cut(s) 644, 847, 1275
RseI CAYNNNNRTG 2 cut(s) 225, 989
SacI GAGCTC 2 cut(s) 313, 676
SaqAI TTAA 4 cut(s) 302, 1053, 1320, 1358
SatI GCNGC 7 cut(s) 67, 489, 801, 828, 975, 978, 1383
Sau3AI GATC 5 cut(s) 76, 189, 466, 588, 603
Sau96I GGNCC 1 cut(s) 267
ScaI AGTACT 1 cut(s) 848
SchI GAGTC 2 cut(s) 1042, 1414
ScrFI CCNGG 2 cut(s) 140, 516
SduI GDGCHC 3 cut(s) 41, 313, 676
SexAI ACCWGGT 1 cut(s) 138
SfaNI GCATC 3 cut(s) 699, 876, 1438
SfcI CTRYAG 2 cut(s) 27, 420
SmiMI CAYNNNNRTG 2 cut(s) 225, 989
SmlI CTYRAG 3 cut(s) 233, 314, 1052
SmoI CTYRAG 3 cut(s) 233, 314, 1052
SsiI CCGC 3 cut(s) 66, 1383, 1408
SspMI CTAG 2 cut(s) 680, 1094
SstI GAGCTC 2 cut(s) 313, 676
StyD4I CCNGG 2 cut(s) 138, 514
StyI CCWWGG 2 cut(s) 70, 223
TaaI ACNGT 6 cut(s) 16, 51, 114, 274, 324, 1300
TaqI TCGA 2 cut(s) 695, 773
TatI WGTACW 1 cut(s) 846
TauI GCSGC 2 cut(s) 69, 1385
TfiI GAWTC 6 cut(s) 23, 163, 511, 779, 862, 1199
Tru1I TTAA 4 cut(s) 302, 1053, 1320, 1358
Tru9I TTAA 4 cut(s) 302, 1053, 1320, 1358
TscAI CASTG 8 cut(s) 46, 327, 606, 615, 976, 1135, 1144, 1410
TseFI GTSAC 4 cut(s) 218, 1045, 1103, 1130
TseI GCWGC 5 cut(s) 488, 800, 827, 974, 977
Tsp45I GTSAC 4 cut(s) 218, 1045, 1103, 1130
TspDTI ATGAA 6 cut(s) 252, 449, 515, 526, 1014, 1458
TspGWI ACGGA 1 cut(s) 562
TspRI CASTG 8 cut(s) 46, 327, 606, 615, 976, 1135, 1144, 1410
Van91I CCANNNNNTGG 1 cut(s) 264
Vha464I CTTAAG 1 cut(s) 1052
XapI RAATTY 2 cut(s) 474, 503
XceI RCATGY 2 cut(s) 687, 1308
XcmI CCANNNNNNNNNTGG 1 cut(s) 1036
XspI CTAG 2 cut(s) 680, 1094
ZrmI AGTACT 1 cut(s) 848
Zsp2I ATGCAT 1 cut(s) 543
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.