Rh5BG229400

MATE efflux family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000715
Physical Location & Seq
Reverse (-)
26927944 .. 26932631
4688 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG229400.1

Sequence Viewer

Length: 1296 bp
ATGTTTGCTGGCCACCTTGGTGAGCTTCCCTTAGCTGGTGCAACCTTGGCCAATTCATGGGCCAATGTTACAGGTTTTGCATTTGTGGTTGGATTAAGTGGAGCTCTTGAGACACTTTGTGGGCAAGGGTTTGGTGCAAAGTCATATAGAATGTTGGGGATTTATCTACAAGCTTCTTGTATCATATCTTTCCTATTCTGCTCCATCATATCTGTAATTTGGTTGTATACCGAACCAATACTTATTTTCCTCCATCAAGATCCTCAAATTGCAAAATCTGCAGCTCTTTTCTTGAAATTTCTAATTCCCGGATTATTTGCATATGGCCTCCTGCAAAACATCTTGAGATTCCTTCAGACACAATCCATCTATGTCATGCCAATGGTCTTCTCCATGATCTCCATTGTAATTCATATTGCCATCACTTATGGTTTAGTACATTGGACATCACTTGGTTTCAAGGGAGCTCCACTAGCAGCTTCAGTTACTCTGTGGATATCGGTTCTTATGTTGGCCTTCCATGTTAGTTTTGCAAAGAATTTTGAGCATACATGGGAAGGATTTTCATTAGAACCATTCCAATATATCATGACTGGTTTGAAACTTGCCCTTCCCTCTGCAACAATGGAATGTTTGGAGGAGTGGGCTTTCGAGATTCTTGTGTTTATGGGAGGATTGATGCCAAACTCGACGAGAACAACTTCACTACTTGCAATGTGTGTGAACACACAAGAAATTGGTTACATGGTTACATATGGCCTCAGTGTTACTGCTAGTACAAGGGTGTCAAATGAATTGGGGGCAGGCAACCCGGGTCGAGCAAAGAATGCAATGCTTGTGACTCTTAAGCTCTCACTGATTCTTTCTTTCACAATTGTTCTGGCTTTGACATTTGGTCACAATATCTGGGCACGCTTCTTCATTGACAGCAGTTCAAATTATTCAGTACTGATAGAGGATTTTGCTTCTATGACACCACTTCTTGCAGTTTCAATAATTCTTGATTCCTTGCAAGGTGTCTTTTCAGGTGTGGCAAGAGGATGTGGGTGGCAGCACTTGGCTGTGTATGTGAACTTGGGAACATTCTACTTTATTGGCATGACAATTGCTGGTCTTCTTGGATTTGAGTTCAAACTTTATGCCAAGGGCTTATGGATTGGTTTAATCTGTGGTCTCTTCTGTCAAGCAAGCACACTGTTGTTGATTACACAGCTCAAGAAATGGACATCTGATTTCTCTCAAAATCCCAAAGAAAGAGAACCAATTTTGGAGCCTAGCTACATTAGTGTGGCATAG

Protein Analysis

431

Amino Acids

47.29

Weight (kDa)

6.7

Isoelectric Point (pI)

28.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 1 - 143 3.9e-30 MatE
MatE PF01554 205 - 368 4e-21 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000715)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23550 AT3G23560 AT3G23560
fragaria_vesca FvH4_1g15630 FvH4_1g15630 FvH4_3g19700 FvH4_3g19700 FvH4_3g19700 FvH4_3g19700 FvH4_3g19700 FvH4_3g19720 FvH4_3g20060 FvH4_3g20060 FvH4_3g20060 FvH4_3g20060 FvH4_5g31740 FvH4_7g19991
malus_domestica MD03G1230300.v1.1 MD11G1250700.v1.1 MD11G1251300.v1.1 MD11G1251400.v1.1 MD11G1251500.v1.1 MD12G1088700.v1.1
prunus_persica Prupe.4G177700_v2.0.a1 Prupe.4G177700_v2.0.a1 Prupe.4G177900_v2.0.a1 Prupe.4G178700_v2.0.a1 Prupe.4G178700_v2.0.a1 Prupe.4G178700_v2.0.a1 Prupe.4G178700_v2.0.a1 Prupe.4G178700_v2.0.a1 Prupe.4G178700_v2.0.a1 Prupe.4G178700_v2.0.a1 Prupe.4G178700_v2.0.a1 Prupe.4G178700_v2.0.a1 Prupe.4G178700_v2.0.a1 Prupe.4G178700_v2.0.a1 Prupe.4G178700_v2.0.a1 Prupe.4G178900_v2.0.a1 Prupe.4G178900_v2.0.a1 Prupe.4G178900_v2.0.a1 Prupe.4G206700_v2.0.a1
pyrus_communis pycom03g17780 pycom10g06980 pycom11g22170 pycom11g22270
rosa_chinensis RchiOBHm_Chr5g0033011 RchiOBHm_Chr5g0033061
rosa_laevigata RLG00000033443 RLG00000033445
rosa_multiflora Rmu_sc0000986.1_g000015
rosa_roxburghii Rroxscaffold_1G00046880 Rroxscaffold_1G00046890 Rroxscaffold_1G00046990 Rroxscaffold_1G00047000 Rroxscaffold_1G00047040
rosa_rugosa Rorug05G0135000 Rorug05G0135100 Rorug05G0135200 Rorug05G0135500 Rorug05G0135600 Rorug05G0135700 Rorug05G0135800
rosa_samantha Rh5AG227700 Rh5AG228200 Rh5BG229300 Rh5BG229400 Rh5CG256700 Rh5CG257200 Rh5DG233900 Rh5DG234100
rosa_wichuraiana Rw5G020860 Rw5G020880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 57
AccI GTMKAC 1 cut(s) 227
AclWI GGATC 1 cut(s) 254
AcoI YGGCCR 2 cut(s) 10, 48
AcsI RAATTY 2 cut(s) 296, 538
AcuI CTGAAG 2 cut(s) 338, 465
AdeI CACNNNGTG 1 cut(s) 119
AfaI GTAC 3 cut(s) 438, 778, 948
AfiI CCNNNNNNNGG 2 cut(s) 35, 57
AflII CTTAAG 1 cut(s) 845
AgsI TTSAA 6 cut(s) 295, 460, 601, 936, 993, 1132
AhdI GACNNNNNGTC 1 cut(s) 894
AleI CACNNNNGTG 1 cut(s) 18
Alw21I GWGCWC 2 cut(s) 106, 469
Alw26I GTCTC 2 cut(s) 104, 1178
AlwI GGATC 1 cut(s) 254
Ama87I CYCGRG 1 cut(s) 811
AoxI GGCC 6 cut(s) 10, 48, 60, 325, 513, 757
ApeKI GCWGC 3 cut(s) 281, 476, 1051
ApoI RAATTY 2 cut(s) 296, 538
Asp700I GAANNNNTTC 1 cut(s) 700
AspS9I GGNCC 1 cut(s) 60
AsuC2I CCSGG 3 cut(s) 309, 812, 813
AsuHPI GGTGA 1 cut(s) 32
AvaI CYCGRG 1 cut(s) 811
BaeGI GKGCMC 1 cut(s) 913
BalI TGGCCA 2 cut(s) 12, 50
BanII GRGCYC 2 cut(s) 106, 469
BbsI GAAGAC 2 cut(s) 379, 1106
Bbv12I GWGCWC 2 cut(s) 106, 469
BbvI GCAGC 3 cut(s) 293, 488, 1063
BccI CCATC 4 cut(s) 212, 261, 374, 428
BcnI CCSGG 3 cut(s) 309, 812, 813
BcoDI GTCTC 2 cut(s) 104, 1178
BfaI CTAG 3 cut(s) 473, 774, 1275
BfmI CTRYAG 1 cut(s) 279
BfrI CTTAAG 1 cut(s) 845
BisI GCNGC 3 cut(s) 282, 477, 1052
BlsI GCNGC 3 cut(s) 283, 478, 1053
BmcAI AGTACT 1 cut(s) 948
Bme1390I CCNGG 3 cut(s) 309, 812, 813
BmeRI GACNNNNNGTC 1 cut(s) 894
BmeT110I CYCGRG 1 cut(s) 811
BmgT120I GGNCC 1 cut(s) 60
BmiI GGNNCC 1 cut(s) 1272
BmrFI CCNGG 3 cut(s) 309, 812, 813
BmsI GCATC 1 cut(s) 669
BpiI GAAGAC 2 cut(s) 379, 1106
Bpu10I CCTNAGC 1 cut(s) 31
BpuEI CTTGAG 3 cut(s) 128, 364, 1199
BpuMI CCSGG 3 cut(s) 309, 812, 813
BsaI GGTCTC 1 cut(s) 1178
BsaJI CCNNGG 4 cut(s) 16, 45, 811, 1143
Bsc4I CCNNNNNNNGG 2 cut(s) 35, 57
Bse1I ACTGG 1 cut(s) 598
Bse3DI GCAATG 2 cut(s) 720, 837
BseDI CCNNGG 4 cut(s) 16, 45, 811, 1143
BseGI GGATG 1 cut(s) 1046
BseLI CCNNNNNNNGG 2 cut(s) 35, 57
BseMI GCAATG 2 cut(s) 720, 837
BseMII CTCAG 1 cut(s) 775
BseNI ACTGG 1 cut(s) 598
BseRI GAGGAG 1 cut(s) 653
BseSI GKGCMC 1 cut(s) 913
BseXI GCAGC 3 cut(s) 293, 488, 1063
BshFI GGCC 6 cut(s) 12, 50, 62, 327, 515, 759
BsiHKAI GWGCWC 2 cut(s) 106, 469
BsiHKCI CYCGRG 1 cut(s) 811
BsiSI CCGG 2 cut(s) 309, 812
BslI CCNNNNNNNGG 2 cut(s) 35, 57
BsmAI GTCTC 2 cut(s) 104, 1178
BsmI GAATGC 1 cut(s) 832
BsnI GGCC 6 cut(s) 12, 50, 62, 327, 515, 759
Bso31I GGTCTC 1 cut(s) 1178
BsoBI CYCGRG 1 cut(s) 811
Bsp1286I GDGCHC 3 cut(s) 106, 469, 913
Bsp143I GATC 2 cut(s) 259, 396
BspANI GGCC 6 cut(s) 12, 50, 62, 327, 515, 759
BspCNI CTCAG 1 cut(s) 774
BspHI TCATGA 1 cut(s) 588
BspLI GGNNCC 1 cut(s) 1272
BspMAI CTGCAG 1 cut(s) 283
BspPI GGATC 1 cut(s) 254
BspTI CTTAAG 1 cut(s) 845
BspTNI GGTCTC 1 cut(s) 1178
BsrDI GCAATG 2 cut(s) 720, 837
BsrI ACTGG 1 cut(s) 598
BssECI CCNNGG 4 cut(s) 16, 45, 811, 1143
BssMI GATC 2 cut(s) 259, 396
BssNAI GTATAC 1 cut(s) 228
BssT1I CCWWGG 3 cut(s) 16, 45, 1143
Bst1107I GTATAC 1 cut(s) 228
Bst4CI ACNGT 1 cut(s) 1197
Bst6I CTCTTC 1 cut(s) 1181
BstAFI CTTAAG 1 cut(s) 845
BstAPI GCANNNNNTGC 2 cut(s) 278, 827
BstC8I GCNNGC 4 cut(s) 10, 805, 913, 1189
BstDEI CTNAG 2 cut(s) 31, 761
BstF5I GGATG 1 cut(s) 1046
BstKTI GATC 2 cut(s) 262, 399
BstMAI GTCTC 2 cut(s) 104, 1178
BstMBI GATC 2 cut(s) 259, 396
BstMWI GCNNNNNNNGC 4 cut(s) 47, 278, 473, 827
BstSCI CCNGG 3 cut(s) 307, 810, 811
BstSFI CTRYAG 1 cut(s) 279
BstSLI GKGCMC 1 cut(s) 913
BstV1I GCAGC 3 cut(s) 293, 488, 1063
BstV2I GAAGAC 2 cut(s) 379, 1106
BstX2I RGATCY 1 cut(s) 259
BstYI RGATCY 1 cut(s) 259
BstZ17I GTATAC 1 cut(s) 228
BsuRI GGCC 6 cut(s) 12, 50, 62, 327, 515, 759
BtsCI GGATG 1 cut(s) 1046
BtsIMutI CAGTG 3 cut(s) 769, 854, 1193
Cac8I GCNNGC 4 cut(s) 10, 805, 913, 1189
CciI TCATGA 1 cut(s) 588
Cfr13I GGNCC 1 cut(s) 60
Cfr9I CCCGGG 1 cut(s) 811
Csp6I GTAC 3 cut(s) 437, 777, 947
CviAII CATG 8 cut(s) 57, 376, 394, 521, 552, 589, 745, 1099
CviQI GTAC 3 cut(s) 437, 777, 947
DdeI CTNAG 2 cut(s) 31, 761
DpnI GATC 2 cut(s) 261, 398
DpnII GATC 2 cut(s) 259, 396
DraIII CACNNNGTG 1 cut(s) 119
DriI GACNNNNNGTC 1 cut(s) 894
EaeI YGGCCR 2 cut(s) 10, 48
Eam1104I CTCTTC 1 cut(s) 1181
Eam1105I GACNNNNNGTC 1 cut(s) 894
EarI CTCTTC 1 cut(s) 1181
Ecl136II GAGCTC 2 cut(s) 104, 467
Eco130I CCWWGG 3 cut(s) 16, 45, 1143
Eco24I GRGCYC 2 cut(s) 106, 469
Eco31I GGTCTC 1 cut(s) 1178
Eco32I GATATC 1 cut(s) 498
Eco53kI GAGCTC 2 cut(s) 104, 467
Eco57I CTGAAG 2 cut(s) 338, 465
Eco88I CYCGRG 1 cut(s) 811
EcoICRI GAGCTC 2 cut(s) 104, 467
EcoRV GATATC 1 cut(s) 498
EcoT14I CCWWGG 3 cut(s) 16, 45, 1143
EcoT38I GRGCYC 2 cut(s) 106, 469
ErhI CCWWGG 3 cut(s) 16, 45, 1143
FaeI CATG 8 cut(s) 60, 379, 397, 524, 555, 592, 748, 1102
FatI CATG 8 cut(s) 56, 375, 393, 520, 551, 588, 744, 1098
FauNDI CATATG 2 cut(s) 322, 754
FblI GTMKAC 1 cut(s) 227
Fnu4HI GCNGC 3 cut(s) 282, 477, 1052
FokI GGATG 1 cut(s) 1053
FriOI GRGCYC 2 cut(s) 106, 469
Fsp4HI GCNGC 3 cut(s) 282, 477, 1052
FspBI CTAG 3 cut(s) 473, 774, 1275
GluI GCNGC 3 cut(s) 282, 477, 1052
HaeIII GGCC 6 cut(s) 12, 50, 62, 327, 515, 759
HapII CCGG 2 cut(s) 309, 812
Hin1II CATG 8 cut(s) 60, 379, 397, 524, 555, 592, 748, 1102
HindIII AAGCTT 1 cut(s) 171
HinfI GANTC 5 cut(s) 348, 655, 841, 859, 1004
HpaII CCGG 2 cut(s) 309, 812
HphI GGTGA 1 cut(s) 32
Hpy166II GTNNAC 3 cut(s) 228, 724, 1072
Hpy188I TCNGA 2 cut(s) 357, 1231
Hpy188III TCNNGA 8 cut(s) 107, 257, 292, 343, 589, 652, 1001, 1216
Hpy8I GTNNAC 3 cut(s) 228, 724, 1072
Hpy99I CGWCG 1 cut(s) 694
HpyAV CCTTC 4 cut(s) 362, 526, 551, 620
HpyCH4III ACNGT 1 cut(s) 1197
HpyF10VI GCNNNNNNNGC 4 cut(s) 47, 278, 473, 827
HpyF3I CTNAG 2 cut(s) 31, 761
Hsp92II CATG 8 cut(s) 60, 379, 397, 524, 555, 592, 748, 1102
Kzo9I GATC 2 cut(s) 259, 396
LmnI GCTCC 5 cut(s) 101, 206, 464, 472, 1270
Lsp1109I GCAGC 3 cut(s) 293, 488, 1063
LweI GCATC 1 cut(s) 669
MaeI CTAG 3 cut(s) 473, 774, 1275
MaeIII GTNAC 7 cut(s) 67, 484, 740, 748, 766, 838, 896
MalI GATC 2 cut(s) 261, 398
MboI GATC 2 cut(s) 259, 396
MboII GAAGA 4 cut(s) 379, 910, 1106, 1168
MfeI CAATTG 2 cut(s) 873, 1104
MflI RGATCY 1 cut(s) 259
MhlI GDGCHC 3 cut(s) 106, 469, 913
MlsI TGGCCA 2 cut(s) 12, 50
MluNI TGGCCA 2 cut(s) 12, 50
MlyI GAGTC 1 cut(s) 835
MmeI TCCRAC 1 cut(s) 70
MnlI CCTC 9 cut(s) 260, 273, 338, 625, 631, 665, 770, 949, 1031
Mox20I TGGCCA 2 cut(s) 12, 50
MroXI GAANNNNTTC 1 cut(s) 700
MscI TGGCCA 2 cut(s) 12, 50
MseI TTAA 3 cut(s) 95, 846, 1163
MslI CAYNNNNRTG 3 cut(s) 18, 380, 1286
Msp20I TGGCCA 2 cut(s) 12, 50
MspCI CTTAAG 1 cut(s) 845
MspI CCGG 2 cut(s) 309, 812
MspR9I CCNGG 3 cut(s) 309, 812, 813
MunI CAATTG 2 cut(s) 873, 1104
Mva1269I GAATGC 1 cut(s) 832
MwoI GCNNNNNNNGC 4 cut(s) 47, 278, 473, 827
NciI CCSGG 3 cut(s) 309, 812, 813
NdeI CATATG 2 cut(s) 322, 754
NdeII GATC 2 cut(s) 259, 396
NlaIII CATG 8 cut(s) 60, 379, 397, 524, 555, 592, 748, 1102
NlaIV GGNNCC 1 cut(s) 1272
NmuCI GTSAC 2 cut(s) 838, 896
OliI CACNNNNGTG 1 cut(s) 18
PagI TCATGA 1 cut(s) 588
PctI GAATGC 1 cut(s) 832
PdmI GAANNNNTTC 1 cut(s) 700
PfeI GAWTC 4 cut(s) 348, 655, 859, 1004
PflMI CCANNNNNTGG 1 cut(s) 57
PfoI TCCNGGA 1 cut(s) 307
PkrI GCNGC 3 cut(s) 283, 478, 1053
PleI GAGTC 1 cut(s) 835
PpsI GAGTC 1 cut(s) 835
Psp124BI GAGCTC 2 cut(s) 106, 469
PspN4I GGNNCC 1 cut(s) 1272
PspPI GGNCC 1 cut(s) 60
PstI CTGCAG 1 cut(s) 283
PsuI RGATCY 1 cut(s) 259
RsaI GTAC 3 cut(s) 438, 778, 948
RsaNI GTAC 3 cut(s) 437, 777, 947
RseI CAYNNNNRTG 3 cut(s) 18, 380, 1286
SacI GAGCTC 2 cut(s) 106, 469
SaqAI TTAA 3 cut(s) 95, 846, 1163
SatI GCNGC 3 cut(s) 282, 477, 1052
Sau3AI GATC 2 cut(s) 259, 396
Sau96I GGNCC 1 cut(s) 60
ScaI AGTACT 1 cut(s) 948
SchI GAGTC 1 cut(s) 835
ScrFI CCNGG 3 cut(s) 309, 812, 813
SduI GDGCHC 3 cut(s) 106, 469, 913
SfaNI GCATC 1 cut(s) 669
SfcI CTRYAG 1 cut(s) 279
SmaI CCCGGG 1 cut(s) 813
SmiMI CAYNNNNRTG 3 cut(s) 18, 380, 1286
SmlI CTYRAG 4 cut(s) 107, 343, 845, 1214
SmoI CTYRAG 4 cut(s) 107, 343, 845, 1214
SspMI CTAG 3 cut(s) 473, 774, 1275
SstI GAGCTC 2 cut(s) 106, 469
StyD4I CCNGG 3 cut(s) 307, 810, 811
StyI CCWWGG 3 cut(s) 16, 45, 1143
TaaI ACNGT 1 cut(s) 1197
TaqI TCGA 3 cut(s) 651, 689, 817
TatI WGTACW 3 cut(s) 436, 776, 946
TfiI GAWTC 4 cut(s) 348, 655, 859, 1004
Tru1I TTAA 3 cut(s) 95, 846, 1163
Tru9I TTAA 3 cut(s) 95, 846, 1163
TscAI CASTG 3 cut(s) 769, 861, 1200
TseFI GTSAC 2 cut(s) 838, 896
TseI GCWGC 3 cut(s) 281, 476, 1051
Tsp45I GTSAC 2 cut(s) 838, 896
TspDTI ATGAA 5 cut(s) 45, 401, 555, 807, 910
TspMI CCCGGG 1 cut(s) 811
TspRI CASTG 3 cut(s) 769, 861, 1200
Van91I CCANNNNNTGG 1 cut(s) 57
Vha464I CTTAAG 1 cut(s) 845
XapI RAATTY 2 cut(s) 296, 538
XmaI CCCGGG 1 cut(s) 811
XmiI GTMKAC 1 cut(s) 227
XmnI GAANNNNTTC 1 cut(s) 700
XspI CTAG 3 cut(s) 473, 774, 1275
ZrmI AGTACT 1 cut(s) 948
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.