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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AgsI | TTSAA | 2 cut(s) | 50, 178 |
| AluBI | AGCT | 1 cut(s) | 174 |
| AluI | AGCT | 1 cut(s) | 174 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 149 |
| BfmI | CTRYAG | 1 cut(s) | 191 |
| BmsI | GCATC | 1 cut(s) | 118 |
| Bse1I | ACTGG | 1 cut(s) | 47 |
| Bse3DI | GCAATG | 1 cut(s) | 169 |
| BseMI | GCAATG | 1 cut(s) | 169 |
| BseNI | ACTGG | 1 cut(s) | 47 |
| BseRI | GAGGAG | 1 cut(s) | 102 |
| BspHI | TCATGA | 1 cut(s) | 37 |
| BsrDI | GCAATG | 1 cut(s) | 169 |
| BsrI | ACTGG | 1 cut(s) | 47 |
| BstSFI | CTRYAG | 1 cut(s) | 191 |
| CciI | TCATGA | 1 cut(s) | 37 |
| CviAII | CATG | 1 cut(s) | 38 |
| CviJI | RGCY | 2 cut(s) | 96, 174 |
| CviKI_1 | RGCY | 2 cut(s) | 96, 174 |
| FaeI | CATG | 1 cut(s) | 41 |
| FaiI | YATR | 3 cut(s) | 34, 39, 117 |
| FatI | CATG | 1 cut(s) | 37 |
| Hin1II | CATG | 1 cut(s) | 41 |
| HinfI | GANTC | 1 cut(s) | 104 |
| Hpy188III | TCNNGA | 2 cut(s) | 38, 101 |
| Hpy99I | CGWCG | 1 cut(s) | 143 |
| HpyAV | CCTTC | 1 cut(s) | 69 |
| HpyCH4V | TGCA | 2 cut(s) | 69, 162 |
| Hsp92II | CATG | 1 cut(s) | 41 |
| LpnPI | CCDG | 1 cut(s) | 28 |
| LweI | GCATC | 1 cut(s) | 118 |
| MnlI | CCTC | 3 cut(s) | 74, 80, 114 |
| MroXI | GAANNNNTTC | 1 cut(s) | 149 |
| NlaIII | CATG | 1 cut(s) | 41 |
| PagI | TCATGA | 1 cut(s) | 37 |
| PdmI | GAANNNNTTC | 1 cut(s) | 149 |
| PfeI | GAWTC | 1 cut(s) | 104 |
| SetI | ASST | 1 cut(s) | 176 |
| SfaNI | GCATC | 1 cut(s) | 118 |
| SfcI | CTRYAG | 1 cut(s) | 191 |
| SgeI | CNNG | 8 cut(s) | 50, 55, 66, 113, 120, 149, 154, 171 |
| TaqI | TCGA | 2 cut(s) | 100, 138 |
| TfiI | GAWTC | 1 cut(s) | 104 |
| TspDTI | ATGAA | 1 cut(s) | 4 |
| XmnI | GAANNNNTTC | 1 cut(s) | 149 |