RchiOBHm_Chr5g0033061

MATE efflux family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000715
Physical Location & Seq
Reverse (-)
26862067 .. 26864895
2829 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ31218

Sequence Viewer

Length: 1635 bp
ATGATAGGGGAAGTTTTCCAGTTTCTGCATGGAGTTCGTTATACTAAACTAGCAGGTGTCAACGAAGAAGGCCATGGAGAAGAAACATCAACAGGAACATGTTGTAAGATACTATTAGACGTTGAGGAGGCTAAGGAACAGGTCTTGTTTTCGTTGCCAGCAATTCTCACCAATTTATTCTTCTCCTTGATCACATTGGTGTCTGTCATGTTTGCTGGCCACCTTGGTGAGCTTCCCTTAGCTGGTGCAACCTTGGCCAATTCATGGGCCAATGTTACAGGTTTTGCATTTGTGGTTGGATTAAGTGGAGCTCTTGAGACACTTTGTGGGCAAGGGTTTGGTGCAAAGTCATATAGAATGTTGGGGATTTATCTACAAGCTTCTTGTATCATATCTTTCCTATTCTGCTCCATCATATCTGTAATTTGGTTGTATACAGAACCAATACTTATTTTCCTCCATCAAGATCCTCAAATTGCAAAATCTGCAGCTCTTTTCTTGAAATTTCTAATTCCCGGATTATTTGCATATGGCCTCCTGCAAAACATCTTGAGATTCCTTCAGACACAATCCATCTATGTCATGCCAATGGTCTTCTCCATGATCTCCATTGTAATTCATATTGCCATCACTTATGGTTTAGTACATTGGACATCTCTTGGTTTCAAGGGAGCTCCACTAGCAGCTTCAGTTACTCTGTGGATATCGGTTCTTATGTTGGCCTTCCATGTTAGTTTTGCAAAGAATTTTGAGCATACATGGGAAGGATTTTCATTAGAACCATTCCAATATATCATGACTGGTTTGAAACTTGCCCTTCCGTCTGCAACAATGGAATGTTTGGAGGAGTGGGCTTTCGAGATTCTTGTGTTTATGGGAGGATTGATGCCAAACTCGACGAGAACAACTTCACTACTTGCAATGTGTGTGAACACACAAGAAATTGGTTACATGGTTACATATGGCCTCAGTGTTACTGCTAGTACAAGGGTGTCAAATGAATTGGGGGCAGGCAACCCAGGTCGAGCAAAGAATGCAATGCTTGTGACTCTTAAGCTCTCACTGATTCTTTCTTTCACAATTGTTCTGGCTTTGACATTTGGTCACAATATCTGGGCACGCTTCTTCATTGACAGCAGTTCAAATTATTCAGTACTGATAGAGGATTTTGCTTCTATGACACCACTTCTTGCAGTTTCAATAATTCTTGATTCCTTGCAAGGTGTCTTTTCAGGTGTGGCAAGAGGATGTGGGTGGCAGCACTTGGCTGTGTATGTGAACTTGGGAACATTCTACTTTATTGGCATGACAATTGCTGGTCTTCTTGGATTTGAGTTCAAACTTTATGCCAAGGGCTTATGGATTGGTTTAATCTGTGGTCTCTTCTGTCAAGCAAGCACACTGTTGTTGATTACACAGCTCAAGAAATGGACATCTGATTTCTCTCAAAATCCCAAAGAAAGAGAACCAATTTTGGAGCCTAGCTACATAAGTGGCTTGTGGATTGGTTTAGTCTGTGGTCTCTCCTGTCAAGCAAGCACACTGTTGTTGATTACACAGCTCAAGAAATGGACGTATGATTTCTCTCAAAATCCTAAAGAAAAAAAATACCAATTTTGGAGCCTAGCTAGATAA

Protein Analysis

544

Amino Acids

60.04

Weight (kDa)

6.56

Isoelectric Point (pI)

26.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 53 - 212 1.5e-34 MatE
MatE PF01554 274 - 437 6.5e-21 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000715)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23550 AT3G23560 AT3G23560
fragaria_vesca FvH4_1g15630 FvH4_1g15630 FvH4_3g19700 FvH4_3g19700 FvH4_3g19700 FvH4_3g19700 FvH4_3g19700 FvH4_3g19720 FvH4_3g20060 FvH4_3g20060 FvH4_3g20060 FvH4_3g20060 FvH4_5g31740 FvH4_7g19991
malus_domestica MD03G1230300.v1.1 MD11G1250700.v1.1 MD11G1251300.v1.1 MD11G1251400.v1.1 MD11G1251500.v1.1 MD12G1088700.v1.1
prunus_persica Prupe.4G177700_v2.0.a1 Prupe.4G177700_v2.0.a1 Prupe.4G177900_v2.0.a1 Prupe.4G178700_v2.0.a1 Prupe.4G178700_v2.0.a1 Prupe.4G178700_v2.0.a1 Prupe.4G178700_v2.0.a1 Prupe.4G178700_v2.0.a1 Prupe.4G178700_v2.0.a1 Prupe.4G178700_v2.0.a1 Prupe.4G178700_v2.0.a1 Prupe.4G178700_v2.0.a1 Prupe.4G178700_v2.0.a1 Prupe.4G178700_v2.0.a1 Prupe.4G178700_v2.0.a1 Prupe.4G178900_v2.0.a1 Prupe.4G178900_v2.0.a1 Prupe.4G178900_v2.0.a1 Prupe.4G206700_v2.0.a1
pyrus_communis pycom03g17780 pycom10g06980 pycom11g22170 pycom11g22270
rosa_chinensis RchiOBHm_Chr5g0033011 RchiOBHm_Chr5g0033061
rosa_laevigata RLG00000033443 RLG00000033445
rosa_multiflora Rmu_sc0000986.1_g000015
rosa_roxburghii Rroxscaffold_1G00046880 Rroxscaffold_1G00046890 Rroxscaffold_1G00046990 Rroxscaffold_1G00047000 Rroxscaffold_1G00047040
rosa_rugosa Rorug05G0135000 Rorug05G0135100 Rorug05G0135200 Rorug05G0135500 Rorug05G0135600 Rorug05G0135700 Rorug05G0135800
rosa_samantha Rh5AG227700 Rh5AG228200 Rh5BG229300 Rh5BG229400 Rh5CG256700 Rh5CG257200 Rh5DG233900 Rh5DG234100
rosa_wichuraiana Rw5G020860 Rw5G020880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 44
Acc36I ACCTGC 1 cut(s) 44
AccB7I CCANNNNNTGG 1 cut(s) 264
AccI GTMKAC 1 cut(s) 434
AclWI GGATC 1 cut(s) 461
AcoI YGGCCR 2 cut(s) 217, 255
AcsI RAATTY 2 cut(s) 503, 745
AcuI CTGAAG 2 cut(s) 545, 672
AdeI CACNNNGTG 1 cut(s) 326
AfaI GTAC 3 cut(s) 645, 985, 1155
AfiI CCNNNNNNNGG 2 cut(s) 242, 264
AflII CTTAAG 1 cut(s) 1052
AflIII ACRYGT 1 cut(s) 98
AgsI TTSAA 6 cut(s) 502, 667, 808, 1143, 1200, 1339
AhdI GACNNNNNGTC 1 cut(s) 1101
AjnI CCWGG 1 cut(s) 1018
AjuI GAANNNNNNNTTGG 2 cut(s) 164, 196
AleI CACNNNNGTG 2 cut(s) 197, 225
Alw21I GWGCWC 2 cut(s) 313, 676
Alw26I GTCTC 3 cut(s) 311, 1385, 1526
AlwI GGATC 1 cut(s) 461
AlwNI CAGNNNCTG 1 cut(s) 25
AoxI GGCC 7 cut(s) 70, 217, 255, 267, 532, 720, 964
ApeKI GCWGC 3 cut(s) 488, 683, 1258
ApoI RAATTY 2 cut(s) 503, 745
Asp700I GAANNNNTTC 1 cut(s) 907
AspS9I GGNCC 1 cut(s) 267
AsuC2I CCSGG 1 cut(s) 516
AsuHPI GGTGA 2 cut(s) 160, 239
BaeGI GKGCMC 1 cut(s) 1120
BalI TGGCCA 2 cut(s) 219, 257
BanII GRGCYC 2 cut(s) 313, 676
BbsI GAAGAC 2 cut(s) 586, 1313
Bbv12I GWGCWC 2 cut(s) 313, 676
BbvI GCAGC 3 cut(s) 500, 695, 1270
BccI CCATC 4 cut(s) 419, 468, 581, 635
BcgI CGANNNNNNTGC 2 cut(s) 17, 51
BciT130I CCWGG 1 cut(s) 1020
BclI TGATCA 1 cut(s) 189
BcnI CCSGG 1 cut(s) 516
BcoDI GTCTC 3 cut(s) 311, 1385, 1526
BfaI CTAG 6 cut(s) 50, 680, 981, 1482, 1625, 1629
BfmI CTRYAG 1 cut(s) 486
BfrI CTTAAG 1 cut(s) 1052
BfuAI ACCTGC 1 cut(s) 44
BisI GCNGC 3 cut(s) 489, 684, 1259
BlsI GCNGC 3 cut(s) 490, 685, 1260
BmcAI AGTACT 1 cut(s) 1155
Bme1390I CCNGG 2 cut(s) 516, 1020
BmeRI GACNNNNNGTC 1 cut(s) 1101
BmgT120I GGNCC 1 cut(s) 267
BmiI GGNNCC 2 cut(s) 1479, 1622
BmrFI CCNGG 2 cut(s) 516, 1020
BmsI GCATC 1 cut(s) 876
BpiI GAAGAC 2 cut(s) 586, 1313
Bpu10I CCTNAGC 2 cut(s) 132, 238
BpuEI CTTGAG 4 cut(s) 335, 571, 1406, 1547
BpuMI CCSGG 1 cut(s) 516
BsaI GGTCTC 2 cut(s) 1385, 1526
BsaJI CCNNGG 5 cut(s) 73, 223, 252, 1018, 1350
Bsc4I CCNNNNNNNGG 2 cut(s) 242, 264
Bse1I ACTGG 2 cut(s) 19, 805
Bse3DI GCAATG 2 cut(s) 927, 1044
BseBI CCWGG 1 cut(s) 1020
BseDI CCNNGG 5 cut(s) 73, 223, 252, 1018, 1350
BseGI GGATG 1 cut(s) 1253
BseLI CCNNNNNNNGG 2 cut(s) 242, 264
BseMI GCAATG 2 cut(s) 927, 1044
BseMII CTCAG 1 cut(s) 982
BseNI ACTGG 2 cut(s) 19, 805
BseRI GAGGAG 2 cut(s) 140, 860
BseSI GKGCMC 1 cut(s) 1120
BseXI GCAGC 3 cut(s) 500, 695, 1270
BshFI GGCC 7 cut(s) 72, 219, 257, 269, 534, 722, 966
BsiHKAI GWGCWC 2 cut(s) 313, 676
BsiSI CCGG 1 cut(s) 516
BslI CCNNNNNNNGG 2 cut(s) 242, 264
BsmAI GTCTC 3 cut(s) 311, 1385, 1526
BsmI GAATGC 1 cut(s) 1039
BsnI GGCC 7 cut(s) 72, 219, 257, 269, 534, 722, 966
Bso31I GGTCTC 2 cut(s) 1385, 1526
Bsp1286I GDGCHC 3 cut(s) 313, 676, 1120
Bsp143I GATC 3 cut(s) 189, 466, 603
Bsp19I CCATGG 1 cut(s) 73
BspANI GGCC 7 cut(s) 72, 219, 257, 269, 534, 722, 966
BspCNI CTCAG 1 cut(s) 981
BspHI TCATGA 1 cut(s) 795
BspLI GGNNCC 2 cut(s) 1479, 1622
BspMAI CTGCAG 1 cut(s) 490
BspMI ACCTGC 1 cut(s) 44
BspPI GGATC 1 cut(s) 461
BspTI CTTAAG 1 cut(s) 1052
BspTNI GGTCTC 2 cut(s) 1385, 1526
BsrDI GCAATG 2 cut(s) 927, 1044
BsrI ACTGG 2 cut(s) 19, 805
BssECI CCNNGG 5 cut(s) 73, 223, 252, 1018, 1350
BssMI GATC 3 cut(s) 189, 466, 603
BssNAI GTATAC 1 cut(s) 435
BssT1I CCWWGG 4 cut(s) 73, 223, 252, 1350
Bst1107I GTATAC 1 cut(s) 435
Bst2UI CCWGG 1 cut(s) 1020
Bst4CI ACNGT 2 cut(s) 1404, 1545
Bst6I CTCTTC 1 cut(s) 1388
BstAFI CTTAAG 1 cut(s) 1052
BstAPI GCANNNNNTGC 2 cut(s) 485, 1034
BstC8I GCNNGC 6 cut(s) 159, 217, 1012, 1120, 1396, 1537
BstDEI CTNAG 3 cut(s) 132, 238, 968
BstDSI CCRYGG 1 cut(s) 73
BstF5I GGATG 1 cut(s) 1253
BstKTI GATC 3 cut(s) 192, 469, 606
BstMAI GTCTC 3 cut(s) 311, 1385, 1526
BstMBI GATC 3 cut(s) 189, 466, 603
BstMWI GCNNNNNNNGC 4 cut(s) 254, 485, 680, 1034
BstNI CCWGG 1 cut(s) 1020
BstNSI RCATGY 1 cut(s) 102
BstSCI CCNGG 2 cut(s) 514, 1018
BstSFI CTRYAG 1 cut(s) 486
BstSLI GKGCMC 1 cut(s) 1120
BstV1I GCAGC 3 cut(s) 500, 695, 1270
BstV2I GAAGAC 2 cut(s) 586, 1313
BstX2I RGATCY 1 cut(s) 466
BstYI RGATCY 1 cut(s) 466
BstZ17I GTATAC 1 cut(s) 435
BsuRI GGCC 7 cut(s) 72, 219, 257, 269, 534, 722, 966
BtgI CCRYGG 1 cut(s) 73
BtsCI GGATG 1 cut(s) 1253
BtsIMutI CAGTG 4 cut(s) 976, 1061, 1400, 1541
BveI ACCTGC 1 cut(s) 44
Cac8I GCNNGC 6 cut(s) 159, 217, 1012, 1120, 1396, 1537
CaiI CAGNNNCTG 1 cut(s) 25
CciI TCATGA 1 cut(s) 795
Cfr13I GGNCC 1 cut(s) 267
Csp6I GTAC 3 cut(s) 644, 984, 1154
CviQI GTAC 3 cut(s) 644, 984, 1154
DdeI CTNAG 3 cut(s) 132, 238, 968
DpnI GATC 3 cut(s) 191, 468, 605
DpnII GATC 3 cut(s) 189, 466, 603
DraIII CACNNNGTG 1 cut(s) 326
DriI GACNNNNNGTC 1 cut(s) 1101
EaeI YGGCCR 2 cut(s) 217, 255
Eam1104I CTCTTC 1 cut(s) 1388
Eam1105I GACNNNNNGTC 1 cut(s) 1101
EarI CTCTTC 1 cut(s) 1388
Ecl136II GAGCTC 2 cut(s) 311, 674
Eco130I CCWWGG 4 cut(s) 73, 223, 252, 1350
Eco24I GRGCYC 2 cut(s) 313, 676
Eco31I GGTCTC 2 cut(s) 1385, 1526
Eco32I GATATC 1 cut(s) 705
Eco53kI GAGCTC 2 cut(s) 311, 674
Eco57I CTGAAG 2 cut(s) 545, 672
EcoICRI GAGCTC 2 cut(s) 311, 674
EcoRII CCWGG 1 cut(s) 1018
EcoRV GATATC 1 cut(s) 705
EcoT14I CCWWGG 4 cut(s) 73, 223, 252, 1350
EcoT38I GRGCYC 2 cut(s) 313, 676
ErhI CCWWGG 4 cut(s) 73, 223, 252, 1350
FauNDI CATATG 2 cut(s) 529, 961
FbaI TGATCA 1 cut(s) 189
FblI GTMKAC 1 cut(s) 434
Fnu4HI GCNGC 3 cut(s) 489, 684, 1259
FokI GGATG 1 cut(s) 1260
FriOI GRGCYC 2 cut(s) 313, 676
Fsp4HI GCNGC 3 cut(s) 489, 684, 1259
FspBI CTAG 6 cut(s) 50, 680, 981, 1482, 1625, 1629
GluI GCNGC 3 cut(s) 489, 684, 1259
HaeIII GGCC 7 cut(s) 72, 219, 257, 269, 534, 722, 966
HapII CCGG 1 cut(s) 516
HincII GTYRAC 1 cut(s) 61
HindII GTYRAC 1 cut(s) 61
HindIII AAGCTT 1 cut(s) 378
HinfI GANTC 5 cut(s) 555, 862, 1048, 1066, 1211
HpaII CCGG 1 cut(s) 516
HphI GGTGA 2 cut(s) 160, 239
Hpy166II GTNNAC 4 cut(s) 61, 435, 931, 1279
Hpy188I TCNGA 2 cut(s) 564, 1438
Hpy188III TCNNGA 9 cut(s) 314, 464, 499, 550, 796, 859, 1208, 1423, 1564
Hpy8I GTNNAC 4 cut(s) 61, 435, 931, 1279
Hpy99I CGWCG 1 cut(s) 901
HpyAV CCTTC 5 cut(s) 62, 569, 733, 758, 827
HpyCH4III ACNGT 2 cut(s) 1404, 1545
HpyCH4IV ACGT 2 cut(s) 120, 1574
HpyF10VI GCNNNNNNNGC 4 cut(s) 254, 485, 680, 1034
HpyF3I CTNAG 3 cut(s) 132, 238, 968
HpySE526I ACGT 2 cut(s) 120, 1574
Ksp22I TGATCA 1 cut(s) 189
Kzo9I GATC 3 cut(s) 189, 466, 603
LmnI GCTCC 6 cut(s) 308, 413, 671, 679, 1477, 1620
Lsp1109I GCAGC 3 cut(s) 500, 695, 1270
LweI GCATC 1 cut(s) 876
MaeI CTAG 6 cut(s) 50, 680, 981, 1482, 1625, 1629
MaeII ACGT 2 cut(s) 120, 1574
MaeIII GTNAC 7 cut(s) 274, 691, 947, 955, 973, 1045, 1103
MalI GATC 3 cut(s) 191, 468, 605
MboI GATC 3 cut(s) 189, 466, 603
MboII GAAGA 7 cut(s) 77, 92, 172, 586, 1117, 1313, 1375
MfeI CAATTG 2 cut(s) 1080, 1311
MflI RGATCY 1 cut(s) 466
MhlI GDGCHC 3 cut(s) 313, 676, 1120
MlsI TGGCCA 2 cut(s) 219, 257
MluNI TGGCCA 2 cut(s) 219, 257
MlyI GAGTC 1 cut(s) 1042
MmeI TCCRAC 1 cut(s) 277
Mox20I TGGCCA 2 cut(s) 219, 257
MroXI GAANNNNTTC 1 cut(s) 907
MscI TGGCCA 2 cut(s) 219, 257
MseI TTAA 3 cut(s) 302, 1053, 1370
MslI CAYNNNNRTG 3 cut(s) 197, 225, 587
Msp20I TGGCCA 2 cut(s) 219, 257
MspCI CTTAAG 1 cut(s) 1052
MspI CCGG 1 cut(s) 516
MspR9I CCNGG 2 cut(s) 516, 1020
MunI CAATTG 2 cut(s) 1080, 1311
Mva1269I GAATGC 1 cut(s) 1039
MvaI CCWGG 1 cut(s) 1020
MwoI GCNNNNNNNGC 4 cut(s) 254, 485, 680, 1034
NciI CCSGG 1 cut(s) 516
NcoI CCATGG 1 cut(s) 73
NdeI CATATG 2 cut(s) 529, 961
NdeII GATC 3 cut(s) 189, 466, 603
NlaIV GGNNCC 2 cut(s) 1479, 1622
NmuCI GTSAC 2 cut(s) 1045, 1103
NspI RCATGY 1 cut(s) 102
OliI CACNNNNGTG 2 cut(s) 197, 225
PagI TCATGA 1 cut(s) 795
PaqCI CACCTGC 1 cut(s) 44
PciI ACATGT 1 cut(s) 98
PctI GAATGC 1 cut(s) 1039
PdmI GAANNNNTTC 1 cut(s) 907
PfeI GAWTC 4 cut(s) 555, 862, 1066, 1211
PflMI CCANNNNNTGG 1 cut(s) 264
PfoI TCCNGGA 1 cut(s) 514
PkrI GCNGC 3 cut(s) 490, 685, 1260
PleI GAGTC 1 cut(s) 1042
PpsI GAGTC 1 cut(s) 1042
PscI ACATGT 1 cut(s) 98
Psp124BI GAGCTC 2 cut(s) 313, 676
Psp6I CCWGG 1 cut(s) 1018
PspGI CCWGG 1 cut(s) 1018
PspN4I GGNNCC 2 cut(s) 1479, 1622
PspPI GGNCC 1 cut(s) 267
PstI CTGCAG 1 cut(s) 490
PstNI CAGNNNCTG 1 cut(s) 25
PsuI RGATCY 1 cut(s) 466
RsaI GTAC 3 cut(s) 645, 985, 1155
RsaNI GTAC 3 cut(s) 644, 984, 1154
RseI CAYNNNNRTG 3 cut(s) 197, 225, 587
SacI GAGCTC 2 cut(s) 313, 676
SaqAI TTAA 3 cut(s) 302, 1053, 1370
SatI GCNGC 3 cut(s) 489, 684, 1259
Sau3AI GATC 3 cut(s) 189, 466, 603
Sau96I GGNCC 1 cut(s) 267
ScaI AGTACT 1 cut(s) 1155
SchI GAGTC 1 cut(s) 1042
ScrFI CCNGG 2 cut(s) 516, 1020
SduI GDGCHC 3 cut(s) 313, 676, 1120
SfaNI GCATC 1 cut(s) 876
SfcI CTRYAG 1 cut(s) 486
SmiMI CAYNNNNRTG 3 cut(s) 197, 225, 587
SmlI CTYRAG 5 cut(s) 314, 550, 1052, 1421, 1562
SmoI CTYRAG 5 cut(s) 314, 550, 1052, 1421, 1562
SspMI CTAG 6 cut(s) 50, 680, 981, 1482, 1625, 1629
SstI GAGCTC 2 cut(s) 313, 676
StyD4I CCNGG 2 cut(s) 514, 1018
StyI CCWWGG 4 cut(s) 73, 223, 252, 1350
TaaI ACNGT 2 cut(s) 1404, 1545
TaiI ACGT 2 cut(s) 123, 1577
TaqI TCGA 3 cut(s) 858, 896, 1024
TatI WGTACW 3 cut(s) 643, 983, 1153
TfiI GAWTC 4 cut(s) 555, 862, 1066, 1211
Tru1I TTAA 3 cut(s) 302, 1053, 1370
Tru9I TTAA 3 cut(s) 302, 1053, 1370
TscAI CASTG 4 cut(s) 976, 1068, 1407, 1548
TseFI GTSAC 2 cut(s) 1045, 1103
TseI GCWGC 3 cut(s) 488, 683, 1258
Tsp45I GTSAC 2 cut(s) 1045, 1103
TspDTI ATGAA 5 cut(s) 252, 608, 762, 1014, 1117
TspGWI ACGGA 1 cut(s) 810
TspRI CASTG 4 cut(s) 976, 1068, 1407, 1548
Van91I CCANNNNNTGG 1 cut(s) 264
Vha464I CTTAAG 1 cut(s) 1052
XapI RAATTY 2 cut(s) 503, 745
XceI RCATGY 1 cut(s) 102
XcmI CCANNNNNNNNNTGG 1 cut(s) 26
XmiI GTMKAC 1 cut(s) 434
XmnI GAANNNNTTC 1 cut(s) 907
XspI CTAG 6 cut(s) 50, 680, 981, 1482, 1625, 1629
ZrmI AGTACT 1 cut(s) 1155
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.