pycom02g23260

Angio-associated migratory cell

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0001404
Physical Location & Seq
Reverse (-)
21377912 .. 21381955
4044 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g23260.2

Sequence Viewer

Length: 1080 bp
ATGACGAAGATCTTCCTGATGCAGATCGATCAAAGTGTTGATGAGCCTGATGACGATGACTCCGTTCACACGTTTACTGGCCATACGGGTGAGCTTTACACAGTTGTGTGCAGCCCAACAGATCCTACTTTTGTTGCAACCGGGGGTGGAGATGACAGAGGGTTTCTTTGGAGAATTGGAGTAGGAGATTTTAATTTTGAGCTCCAAGGTCATAAGGATTCTGTTTCTAGTTTATCATTTAGTACTGATGGGAAGTTGCTTGCATCCGGAAGCTTGGATGGAACTATTCAAATTTGGGATGTAGCTTACGGAGTTGGAAAAAGAACTCTTGAAGGTCCTGGAGGGGGCATCGAGTGGGTCAGGTGGCATCCTAGAGGGCATCTGGTCCTGGCTGGTTCAGAGGACTGCACTGTCTGGATGTGGAATGCTGACAGTGGTTCCTATCTTAATATGTTTTCAGGCCATGGTAGTAACGTGAGTTGTGGTGATTTTACCCCTGATGGTAAAACAATATGCACTGGTTCAGCTGATGCAACTTTGAGAATATGGAATCCAAAAAGTGGCGAAAACATTCATGTCATACAAGGTCACCCATACCATACTGCTGGTTTAACATGTTTGGCAATAAGCTCTGATTCAACTCTAGCTTATACTGGTGCTGAAGATGGTTCTATCCGTATCGTCAACATAACTACCGGAAAGGTTGTTAATTCCCTGGCTTCACATTCAGATTCTGTCGAATGCATCGGGCTTGCACCAAGTTCCCCTTGGGCTGCAACAGGAGGTATGGATAAGAAGCTTGTTATCTGGGACTTGCCTTCTTCATCGGCCCGTGCTACATGTGAACATGAGGATGGAGTGACATGCTTGACATGGCTTGGTCAATCAAATTACCTAGCAACAGGTTGTGGGGATGGAAAGGTTCGAGTCTGGGACAGTCGCTCTGGGGATTGTGTTAGAACCTTCAGCGGCCACACCGAAACTATACAGTCTCTCTCCGTTTCTGCCAACCAAGAGTTTCTTGTTTCAGTATCTACTGATGGAACTGCCCGAGTTTTTGAGATTGCAGAATACAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

360

Amino Acids

38.31

Weight (kDa)

4.76

Isoelectric Point (pI)

35.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 410
AccB7I CCANNNNNTGG 1 cut(s) 560
AccIII TCCGGA 1 cut(s) 266
AciI CCGC 1 cut(s) 969
AclWI GGATC 1 cut(s) 116
AcoI YGGCCR 2 cut(s) 79, 970
AcsI RAATTY 1 cut(s) 291
AcuI CTGAAG 2 cut(s) 681, 949
AfaI GTAC 1 cut(s) 244
AfiI CCNNNNNNNGG 2 cut(s) 344, 560
AflIII ACRYGT 3 cut(s) 69, 614, 839
AgsI TTSAA 3 cut(s) 290, 332, 639
AjnI CCWGG 3 cut(s) 337, 387, 714
AleI CACNNNNGTG 1 cut(s) 104
AluBI AGCT 8 cut(s) 94, 202, 273, 305, 527, 630, 647, 799
AluI AGCT 8 cut(s) 94, 202, 273, 305, 527, 630, 647, 799
Alw21I GWGCWC 1 cut(s) 204
Alw26I GTCTC 1 cut(s) 996
AlwI GGATC 1 cut(s) 116
AlwNI CAGNNNCTG 1 cut(s) 734
Ama87I CYCGRG 1 cut(s) 1050
Aor13HI TCCGGA 1 cut(s) 266
AoxI GGCC 4 cut(s) 79, 460, 828, 970
ApeKI GCWGC 2 cut(s) 111, 773
ApoI RAATTY 1 cut(s) 291
Asp700I GAANNNNTTC 2 cut(s) 11, 570
AspS9I GGNCC 3 cut(s) 335, 385, 829
AsuC2I CCSGG 1 cut(s) 142
AsuHPI GGTGA 3 cut(s) 101, 497, 581
AvaI CYCGRG 1 cut(s) 1050
AvaII GGWCC 2 cut(s) 335, 385
BalI TGGCCA 1 cut(s) 81
BanII GRGCYC 1 cut(s) 204
Bbv12I GWGCWC 1 cut(s) 204
BbvI GCAGC 2 cut(s) 123, 760
BccI CCATC 7 cut(s) 242, 272, 494, 659, 848, 908, 1034
BciT130I CCWGG 3 cut(s) 339, 389, 716
BcnI CCSGG 1 cut(s) 142
BcoDI GTCTC 1 cut(s) 996
BfaI CTAG 4 cut(s) 228, 372, 644, 896
BglII AGATCT 1 cut(s) 9
BisI GCNGC 3 cut(s) 112, 774, 970
BlsI GCNGC 3 cut(s) 113, 775, 971
BmcAI AGTACT 1 cut(s) 244
Bme1390I CCNGG 4 cut(s) 142, 339, 389, 716
Bme18I GGWCC 2 cut(s) 335, 385
BmeT110I CYCGRG 1 cut(s) 1050
BmgT120I GGNCC 3 cut(s) 335, 385, 829
BmiI GGNNCC 1 cut(s) 439
BmrFI CCNGG 4 cut(s) 142, 339, 389, 716
BmsI GCATC 7 cut(s) 9, 272, 357, 376, 388, 520, 753
BpmI CTGGAG 1 cut(s) 360
BpuMI CCSGG 1 cut(s) 142
Bsa29I ATCGAT 1 cut(s) 27
BsaBI GATNNNNATC 1 cut(s) 23
BsaJI CCNNGG 5 cut(s) 141, 205, 463, 714, 767
BsaWI WCCGGW 2 cut(s) 266, 695
BsaXI ACNNNNNCTCC 2 cut(s) 44, 74
Bsc4I CCNNNNNNNGG 2 cut(s) 344, 560
Bse1I ACTGG 3 cut(s) 82, 523, 658
Bse8I GATNNNNATC 1 cut(s) 23
BseAI TCCGGA 1 cut(s) 266
BseBI CCWGG 3 cut(s) 339, 389, 716
BseCI ATCGAT 1 cut(s) 27
BseDI CCNNGG 5 cut(s) 141, 205, 463, 714, 767
BseGI GGATG 7 cut(s) 263, 283, 304, 367, 423, 859, 919
BseJI GATNNNNATC 1 cut(s) 23
BseLI CCNNNNNNNGG 2 cut(s) 344, 560
BseNI ACTGG 3 cut(s) 82, 523, 658
BseXI GCAGC 2 cut(s) 123, 760
BsgI GTGCAG 2 cut(s) 130, 391
BshFI GGCC 4 cut(s) 81, 462, 830, 972
BshVI ATCGAT 1 cut(s) 27
BsiHKAI GWGCWC 1 cut(s) 204
BsiHKCI CYCGRG 1 cut(s) 1050
BsiSI CCGG 3 cut(s) 141, 267, 696
BslFI GGGAC 2 cut(s) 824, 947
BslI CCNNNNNNNGG 2 cut(s) 344, 560
BsmAI GTCTC 1 cut(s) 996
BsmFI GGGAC 2 cut(s) 824, 947
BsmI GAATGC 2 cut(s) 430, 746
BsnI GGCC 4 cut(s) 81, 462, 830, 972
BsoBI CYCGRG 1 cut(s) 1050
Bsp1286I GDGCHC 1 cut(s) 204
Bsp13I TCCGGA 1 cut(s) 266
Bsp143I GATC 4 cut(s) 9, 24, 28, 121
Bsp19I CCATGG 1 cut(s) 463
BspACI CCGC 1 cut(s) 969
BspANI GGCC 4 cut(s) 81, 462, 830, 972
BspDI ATCGAT 1 cut(s) 27
BspEI TCCGGA 1 cut(s) 266
BspLI GGNNCC 1 cut(s) 439
BspPI GGATC 1 cut(s) 116
BsrI ACTGG 3 cut(s) 82, 523, 658
BssECI CCNNGG 5 cut(s) 141, 205, 463, 714, 767
BssMI GATC 4 cut(s) 9, 24, 28, 121
BssT1I CCWWGG 3 cut(s) 205, 463, 767
Bst2UI CCWGG 3 cut(s) 339, 389, 716
Bst4CI ACNGT 5 cut(s) 103, 412, 434, 938, 990
BstC8I GCNNGC 2 cut(s) 261, 753
BstDSI CCRYGG 1 cut(s) 463
BstEII GGTNACC 1 cut(s) 587
BstF5I GGATG 7 cut(s) 263, 283, 304, 367, 423, 859, 919
BstKTI GATC 4 cut(s) 12, 27, 31, 124
BstMAI GTCTC 1 cut(s) 996
BstMBI GATC 4 cut(s) 9, 24, 28, 121
BstNI CCWGG 3 cut(s) 339, 389, 716
BstNSI RCATGY 3 cut(s) 618, 843, 867
BstPI GGTNACC 1 cut(s) 587
BstSCI CCNGG 4 cut(s) 140, 337, 387, 714
BstV1I GCAGC 2 cut(s) 123, 760
BstX2I RGATCY 2 cut(s) 9, 121
BstXI CCANNNNNNTGG 1 cut(s) 605
BstYI RGATCY 2 cut(s) 9, 121
Bsu15I ATCGAT 1 cut(s) 27
BsuRI GGCC 4 cut(s) 81, 462, 830, 972
BsuTUI ATCGAT 1 cut(s) 27
BtgI CCRYGG 1 cut(s) 463
BtsCI GGATG 7 cut(s) 263, 283, 304, 367, 423, 859, 919
BtsIMutI CAGTG 3 cut(s) 408, 439, 516
Cac8I GCNNGC 2 cut(s) 261, 753
CaiI CAGNNNCTG 1 cut(s) 734
Cfr13I GGNCC 3 cut(s) 335, 385, 829
ClaI ATCGAT 1 cut(s) 27
Csp6I GTAC 1 cut(s) 243
CviAII CATG 7 cut(s) 464, 575, 615, 840, 848, 864, 873
CviQI GTAC 1 cut(s) 243
DpnI GATC 4 cut(s) 11, 26, 30, 123
DpnII GATC 4 cut(s) 9, 24, 28, 121
DrdI GACNNNNNNGTC 1 cut(s) 410
DseDI GACNNNNNNGTC 1 cut(s) 410
EaeI YGGCCR 2 cut(s) 79, 970
Ecl136II GAGCTC 1 cut(s) 202
Eco130I CCWWGG 3 cut(s) 205, 463, 767
Eco24I GRGCYC 1 cut(s) 204
Eco47I GGWCC 2 cut(s) 335, 385
Eco53kI GAGCTC 1 cut(s) 202
Eco57I CTGAAG 2 cut(s) 681, 949
Eco88I CYCGRG 1 cut(s) 1050
Eco91I GGTNACC 1 cut(s) 587
EcoICRI GAGCTC 1 cut(s) 202
EcoO109I RGGNCCY 1 cut(s) 335
EcoO65I GGTNACC 1 cut(s) 587
EcoRII CCWGG 3 cut(s) 337, 387, 714
EcoT14I CCWWGG 3 cut(s) 205, 463, 767
EcoT22I ATGCAT 1 cut(s) 746
EcoT38I GRGCYC 1 cut(s) 204
ErhI CCWWGG 3 cut(s) 205, 463, 767
FaeI CATG 7 cut(s) 467, 578, 618, 843, 851, 867, 876
FalI AAGNNNNNCTT 4 cut(s) 751, 783, 1005, 1037
FaqI GGGAC 2 cut(s) 824, 947
FatI CATG 7 cut(s) 463, 574, 614, 839, 847, 863, 872
Fnu4HI GCNGC 3 cut(s) 112, 774, 970
FokI GGATG 7 cut(s) 250, 290, 311, 354, 430, 866, 926
FriOI GRGCYC 1 cut(s) 204
Fsp4HI GCNGC 3 cut(s) 112, 774, 970
FspBI CTAG 4 cut(s) 228, 372, 644, 896
GluI GCNGC 3 cut(s) 112, 774, 970
GsuI CTGGAG 1 cut(s) 360
HaeIII GGCC 4 cut(s) 81, 462, 830, 972
HapII CCGG 3 cut(s) 141, 267, 696
Hin1II CATG 7 cut(s) 467, 578, 618, 843, 851, 867, 876
HincII GTYRAC 1 cut(s) 685
HindII GTYRAC 1 cut(s) 685
HindIII AAGCTT 2 cut(s) 271, 797
HinfI GANTC 6 cut(s) 59, 218, 550, 635, 731, 927
HpaII CCGG 3 cut(s) 141, 267, 696
HphI GGTGA 3 cut(s) 101, 497, 581
Hpy166II GTNNAC 4 cut(s) 67, 75, 685, 845
Hpy188I TCNGA 3 cut(s) 400, 634, 730
Hpy188III TCNNGA 4 cut(s) 16, 267, 329, 415
Hpy8I GTNNAC 4 cut(s) 67, 75, 685, 845
HpyAV CCTTC 3 cut(s) 326, 828, 973
HpyCH4III ACNGT 5 cut(s) 103, 412, 434, 938, 990
HpyCH4IV ACGT 2 cut(s) 71, 474
HpySE526I ACGT 2 cut(s) 71, 474
Hsp92II CATG 7 cut(s) 467, 578, 618, 843, 851, 867, 876
Kpn2I TCCGGA 1 cut(s) 266
Kzo9I GATC 4 cut(s) 9, 24, 28, 121
LmnI GCTCC 1 cut(s) 207
Lsp1109I GCAGC 2 cut(s) 123, 760
LweI GCATC 7 cut(s) 9, 272, 357, 376, 388, 520, 753
MaeI CTAG 4 cut(s) 228, 372, 644, 896
MaeII ACGT 2 cut(s) 71, 474
MaeIII GTNAC 3 cut(s) 470, 587, 859
MalI GATC 4 cut(s) 11, 26, 30, 123
MboI GATC 4 cut(s) 9, 24, 28, 121
MboII GAAGA 4 cut(s) 4, 19, 674, 813
MflI RGATCY 2 cut(s) 9, 121
MhlI GDGCHC 1 cut(s) 204
MlsI TGGCCA 1 cut(s) 81
MluCI AATT 5 cut(s) 174, 193, 291, 709, 889
MluNI TGGCCA 1 cut(s) 81
MlyI GAGTC 2 cut(s) 53, 936
MmeI TCCRAC 1 cut(s) 295
MnlI CCTC 6 cut(s) 152, 335, 368, 394, 776, 844
Mox20I TGGCCA 1 cut(s) 81
Mph1103I ATGCAT 1 cut(s) 746
MroI TCCGGA 1 cut(s) 266
MroXI GAANNNNTTC 2 cut(s) 11, 570
MscI TGGCCA 1 cut(s) 81
MseI TTAA 4 cut(s) 192, 447, 611, 708
MslI CAYNNNNRTG 3 cut(s) 87, 104, 852
Msp20I TGGCCA 1 cut(s) 81
MspA1I CMGCKG 2 cut(s) 527, 969
MspI CCGG 3 cut(s) 141, 267, 696
MspR9I CCNGG 4 cut(s) 142, 339, 389, 716
Mva1269I GAATGC 2 cut(s) 430, 746
MvaI CCWGG 3 cut(s) 339, 389, 716
NciI CCSGG 1 cut(s) 142
NcoI CCATGG 1 cut(s) 463
NdeII GATC 4 cut(s) 9, 24, 28, 121
NlaIII CATG 7 cut(s) 467, 578, 618, 843, 851, 867, 876
NlaIV GGNNCC 1 cut(s) 439
NmuCI GTSAC 2 cut(s) 587, 859
NsiI ATGCAT 1 cut(s) 746
NspI RCATGY 3 cut(s) 618, 843, 867
OliI CACNNNNGTG 1 cut(s) 104
PciI ACATGT 2 cut(s) 614, 839
PcsI WCGNNNNNNNCGW 1 cut(s) 60
PctI GAATGC 2 cut(s) 430, 746
PdmI GAANNNNTTC 2 cut(s) 11, 570
PfeI GAWTC 4 cut(s) 218, 550, 635, 731
PflMI CCANNNNNTGG 1 cut(s) 560
PfoI TCCNGGA 1 cut(s) 337
PkrI GCNGC 3 cut(s) 113, 775, 971
PleI GAGTC 2 cut(s) 53, 935
PpsI GAGTC 2 cut(s) 53, 935
PpuMI RGGWCCY 1 cut(s) 335
PscI ACATGT 2 cut(s) 614, 839
Psp124BI GAGCTC 1 cut(s) 204
Psp5II RGGWCCY 1 cut(s) 335
Psp6I CCWGG 3 cut(s) 337, 387, 714
PspEI GGTNACC 1 cut(s) 587
PspGI CCWGG 3 cut(s) 337, 387, 714
PspN4I GGNNCC 1 cut(s) 439
PspPI GGNCC 3 cut(s) 335, 385, 829
PspPPI RGGWCCY 1 cut(s) 335
PstNI CAGNNNCTG 1 cut(s) 734
PsuI RGATCY 2 cut(s) 9, 121
PvuII CAGCTG 1 cut(s) 527
RsaI GTAC 1 cut(s) 244
RsaNI GTAC 1 cut(s) 243
RseI CAYNNNNRTG 3 cut(s) 87, 104, 852
SacI GAGCTC 1 cut(s) 204
SaqAI TTAA 4 cut(s) 192, 447, 611, 708
SatI GCNGC 3 cut(s) 112, 774, 970
Sau3AI GATC 4 cut(s) 9, 24, 28, 121
Sau96I GGNCC 3 cut(s) 335, 385, 829
ScaI AGTACT 1 cut(s) 244
SchI GAGTC 2 cut(s) 53, 936
ScrFI CCNGG 4 cut(s) 142, 339, 389, 716
SduI GDGCHC 1 cut(s) 204
SfaNI GCATC 7 cut(s) 9, 272, 357, 376, 388, 520, 753
SinI GGWCC 2 cut(s) 335, 385
SmiMI CAYNNNNRTG 3 cut(s) 87, 104, 852
Sse9I AATT 5 cut(s) 174, 193, 291, 709, 889
SsiI CCGC 1 cut(s) 969
SspMI CTAG 4 cut(s) 228, 372, 644, 896
SstI GAGCTC 1 cut(s) 204
StyD4I CCNGG 4 cut(s) 140, 337, 387, 714
StyI CCWWGG 3 cut(s) 205, 463, 767
TaaI ACNGT 5 cut(s) 103, 412, 434, 938, 990
TaiI ACGT 2 cut(s) 74, 477
TaqI TCGA 4 cut(s) 27, 351, 738, 925
TasI AATT 5 cut(s) 174, 193, 291, 709, 889
TatI WGTACW 1 cut(s) 242
TauI GCSGC 1 cut(s) 972
TfiI GAWTC 4 cut(s) 218, 550, 635, 731
Tru1I TTAA 4 cut(s) 192, 447, 611, 708
Tru9I TTAA 4 cut(s) 192, 447, 611, 708
TscAI CASTG 3 cut(s) 415, 439, 523
TseFI GTSAC 2 cut(s) 587, 859
TseI GCWGC 2 cut(s) 111, 773
Tsp45I GTSAC 2 cut(s) 587, 859
TspDTI ATGAA 2 cut(s) 563, 813
TspGWI ACGGA 4 cut(s) 52, 324, 665, 988
TspRI CASTG 3 cut(s) 415, 439, 523
Van91I CCANNNNNTGG 1 cut(s) 560
VpaK11BI GGWCC 2 cut(s) 335, 385
XapI RAATTY 1 cut(s) 291
XceI RCATGY 3 cut(s) 618, 843, 867
XcmI CCANNNNNNNNNTGG 1 cut(s) 765
XmnI GAANNNNTTC 2 cut(s) 11, 570
XspI CTAG 4 cut(s) 228, 372, 644, 896
ZrmI AGTACT 1 cut(s) 244
Zsp2I ATGCAT 1 cut(s) 746
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.