Rw6G016260

Angio-associated migratory cell

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0001404
Physical Location & Seq
Forward (+)
31831263 .. 31835365
4103 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G016260.1

Sequence Viewer

Length: 1170 bp
ATGTACACGCTCTTTAAAACATCTCTAATTCATAAATTTCATTGGAATTACATTGATGAAGCTGACATCCTTAACAAAGTCGATTTCGATGAGGAAGATCTTCCTGATGCAGACGACCAAGTTGTTGATGGGCCTGATGATTCCATGCATACTTTTACTGGTCATACGGGTGAACTTTATACAGTTGTCTGCAGTCTGACAGATCCAGTGTTAGTTGCAACAGGGGGTGGAGATGACAGAGGGTTTTTTGGAAGATTGGACAAGGAGATTGGGCTTCTGAGGTCCAAGGTAGGCCATAAGGATTCTGTTTCAAGTTTAACATTTAGTACTGATGGACAACTGCTTGCATCTGGAAGCTTGGATGGAATTATTCAAATTTGGGATATAACATCAGCAAATCTAAAATTTATTACTTGGGTCAGGTGGCATCCTAGAGGGTATCTGGTCTTGGCTGGTTCGTTCCTATATTCAATTGTTTCAGGGCATGGTGGTAGCGTGACTTGTGCTGATTTTACCCCCGATGGTAAAACAATCTGCACTGGTTCTAATGTTGCAAGTTTGAGAATATGGAACCCAAAAAGTGGAGTGAACATTCATGTGGTACAACGTCATCCTTACCCATTCTGCAGGTTGAAATGCTTGGCAATAAGCTCTGATTCAACTCTAGCTGTTACTGGTTCTGAAGATGGATCTGTCCATATTGTAAATATAACTACTGGAAAGGTTGTTAGTTCCCTGGCTTCACATTCAGATTCAGTAGAATGTATCAGACTTCCACCAAGTTTCCCTTGGGCTGCAACAGGAGGTATGGATAACAAGCTTATCATCTGGGATTTGCAACATACGTTGCCCCGTTCTACATGTGACCATGAGGTACCTAGCAACAGGTTGTGCGGATGGGAAGGTCGATTATGGGATAGTCTCTCTGGTGATTGTGTAAGAACGTTCAATGGGCATAGTGAACCCATTCAGTCTCTCTCTGTATCTGCCAACAAGGACTTCCTTGTTTCAGTATCTACTGATGGAACCGCCCGAGTTTTTGAGATTGAAAAATATAAGCAAGCAATATACCCTTTAGAGTTGGTAACAAGGATGGCCTTGGAGTTATATAGTACTTTTTTGTATAAACAATATGAATTACATGATTTTCTTATGGTATTCCTTTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

389

Amino Acids

43.02

Weight (kDa)

5.3

Isoelectric Point (pI)

37.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_Prp19 PF24814 47 - 133 6.3e-07 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 47 - 148 7.1e-10 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 48 - 133 8.3e-10 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 49 - 132 1.5e-08 WDR3 first beta-propeller domain
WD40 PF00400 95 - 128 9.4e-10 WD domain, G-beta repeat
WD40_WDHD1_1st PF24817 97 - 134 9.8e-06 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 99 - 198 7.3e-07 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_TEP1_2nd PF25047 157 - 280 1.5e-09 TEP-1 second beta-propeller
Beta-prop_WDR5 PF25175 159 - 282 2.7e-19 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 159 - 248 3.1e-12 THOC3 beta-propeller domain
WD40_CDC20-Fz PF24807 162 - 289 2.2e-14 CDC20/Fizzy WD40 domain
WDR55 PF24796 165 - 281 1.1e-07 WDR55
WD40_MABP1-WDR62_2nd PF24782 166 - 259 6.7e-06 MABP1/WDR62 second WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 169 - 281 4.5e-07 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR3_2nd PF25172 170 - 282 4.6e-11 WDR3 second beta-propeller domain
Beta-prop_EML_2 PF23414 171 - 355 3.1e-11 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 178 - 282 5.5e-08 WDR36/Utp21 first beta-propeller
Beta-prop_WDR3_1st PF25173 187 - 280 8.7e-12 WDR3 first beta-propeller domain
NBCH_WD40 PF20426 210 - 345 7.2e-11 Neurobeachin beta propeller domain
WD40_WDHD1_1st PF24817 212 - 283 1.6e-08 WDHD1 first WD40 domain
WD40_Prp19 PF24814 212 - 282 4.6e-08 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 225 - 288 1.6e-06 THOC3 beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 240 - 354 3.8e-08 CAF1B/HIR1 beta-propeller domain
WD40 PF00400 240 - 278 3.7e-06 WD domain, G-beta repeat
WD40_Prp19 PF24814 303 - 354 6.5e-06 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 303 - 354 2.9e-06 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 303 - 352 3.6e-09 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 304 - 350 2e-06 WDR3 first beta-propeller domain
WD40_WDHD1_1st PF24817 310 - 354 5.4e-07 WDHD1 first WD40 domain
WD40 PF00400 310 - 347 2.1e-07 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 618
Acc65I GGTACC 1 cut(s) 874
AccB1I GGYRCC 1 cut(s) 874
AccB7I CCANNNNNTGG 1 cut(s) 581
AciI CCGC 2 cut(s) 894, 1029
AclI AACGTT 1 cut(s) 944
AclWI GGATC 2 cut(s) 197, 697
AcsI RAATTY 3 cut(s) 35, 375, 404
AcuI CTGAAG 1 cut(s) 702
AfaI GTAC 5 cut(s) 5, 328, 603, 876, 1114
AfiI CCNNNNNNNGG 1 cut(s) 581
AflIII ACRYGT 1 cut(s) 860
AgsI TTSAA 7 cut(s) 312, 374, 471, 634, 660, 949, 1049
AjnI CCWGG 1 cut(s) 735
AjuI GAANNNNNNNTTGG 2 cut(s) 983, 1015
AluBI AGCT 5 cut(s) 62, 357, 651, 668, 820
AluI AGCT 5 cut(s) 62, 357, 651, 668, 820
Alw26I GTCTC 2 cut(s) 926, 978
AlwI GGATC 2 cut(s) 197, 697
Ama87I CYCGRG 1 cut(s) 1032
AoxI GGCC 3 cut(s) 131, 292, 1095
ApeKI GCWGC 1 cut(s) 794
ApoI RAATTY 3 cut(s) 35, 375, 404
Asp700I GAANNNNTTC 2 cut(s) 99, 966
Asp718I GGTACC 1 cut(s) 874
AspS9I GGNCC 2 cut(s) 131, 282
AsuHPI GGTGA 2 cut(s) 182, 941
AvaI CYCGRG 1 cut(s) 1032
AvaII GGWCC 1 cut(s) 282
BanI GGYRCC 1 cut(s) 874
BbvI GCAGC 1 cut(s) 781
BccI CCATC 8 cut(s) 122, 326, 356, 515, 680, 891, 1016, 1087
BciT130I CCWGG 1 cut(s) 737
BcoDI GTCTC 2 cut(s) 926, 978
BfaI CTAG 3 cut(s) 432, 665, 879
BfmI CTRYAG 2 cut(s) 190, 625
BfuAI ACCTGC 1 cut(s) 618
BglII AGATCT 1 cut(s) 97
BisI GCNGC 1 cut(s) 795
BlsI GCNGC 1 cut(s) 796
BmcAI AGTACT 2 cut(s) 328, 1114
Bme1390I CCNGG 1 cut(s) 737
Bme18I GGWCC 1 cut(s) 282
BmeT110I CYCGRG 1 cut(s) 1032
BmgT120I GGNCC 2 cut(s) 131, 282
BmiI GGNNCC 3 cut(s) 572, 876, 1027
BmrFI CCNGG 1 cut(s) 737
BmsI GCATC 3 cut(s) 97, 356, 436
BsaJI CCNNGG 4 cut(s) 285, 735, 788, 1098
Bsc4I CCNNNNNNNGG 1 cut(s) 581
Bse1I ACTGG 5 cut(s) 163, 206, 544, 679, 721
BseBI CCWGG 1 cut(s) 737
BseDI CCNNGG 4 cut(s) 285, 735, 788, 1098
BseGI GGATG 6 cut(s) 66, 367, 427, 610, 902, 1098
BseLI CCNNNNNNNGG 1 cut(s) 581
BseMII CTCAG 1 cut(s) 269
BseNI ACTGG 5 cut(s) 163, 206, 544, 679, 721
BseXI GCAGC 1 cut(s) 781
BsgI GTGCAG 1 cut(s) 520
BshFI GGCC 3 cut(s) 133, 294, 1097
BshNI GGYRCC 1 cut(s) 874
BsiHKCI CYCGRG 1 cut(s) 1032
BslI CCNNNNNNNGG 1 cut(s) 581
BsmAI GTCTC 2 cut(s) 926, 978
BsnI GGCC 3 cut(s) 133, 294, 1097
BsoBI CYCGRG 1 cut(s) 1032
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 3 cut(s) 97, 202, 689
BspACI CCGC 2 cut(s) 894, 1029
BspANI GGCC 3 cut(s) 133, 294, 1097
BspCNI CTCAG 1 cut(s) 270
BspLI GGNNCC 3 cut(s) 572, 876, 1027
BspMAI CTGCAG 2 cut(s) 194, 629
BspMI ACCTGC 1 cut(s) 618
BspPI GGATC 2 cut(s) 197, 697
BspT107I GGYRCC 1 cut(s) 874
BsrGI TGTACA 1 cut(s) 3
BsrI ACTGG 5 cut(s) 163, 206, 544, 679, 721
BssECI CCNNGG 4 cut(s) 285, 735, 788, 1098
BssMI GATC 3 cut(s) 97, 202, 689
BssT1I CCWWGG 3 cut(s) 285, 788, 1098
Bst2UI CCWGG 1 cut(s) 737
Bst4CI ACNGT 1 cut(s) 184
BstAUI TGTACA 1 cut(s) 3
BstC8I GCNNGC 2 cut(s) 345, 1062
BstDEI CTNAG 1 cut(s) 278
BstF5I GGATG 6 cut(s) 66, 367, 427, 610, 902, 1098
BstKTI GATC 3 cut(s) 100, 205, 692
BstMAI GTCTC 2 cut(s) 926, 978
BstMBI GATC 3 cut(s) 97, 202, 689
BstNI CCWGG 1 cut(s) 737
BstNSI RCATGY 1 cut(s) 864
BstSCI CCNGG 1 cut(s) 735
BstSFI CTRYAG 2 cut(s) 190, 625
BstV1I GCAGC 1 cut(s) 781
BstX2I RGATCY 3 cut(s) 97, 202, 689
BstYI RGATCY 3 cut(s) 97, 202, 689
BsuRI GGCC 3 cut(s) 133, 294, 1097
BtsCI GGATG 6 cut(s) 66, 367, 427, 610, 902, 1098
BtsIMutI CAGTG 2 cut(s) 213, 537
BveI ACCTGC 1 cut(s) 618
Cac8I GCNNGC 2 cut(s) 345, 1062
Cfr13I GGNCC 2 cut(s) 131, 282
Csp6I GTAC 5 cut(s) 4, 327, 602, 875, 1113
CviAII CATG 6 cut(s) 145, 485, 596, 861, 869, 1142
CviQI GTAC 5 cut(s) 4, 327, 602, 875, 1113
DdeI CTNAG 1 cut(s) 278
DpnI GATC 3 cut(s) 99, 204, 691
DpnII GATC 3 cut(s) 97, 202, 689
DraI TTTAAA 1 cut(s) 16
Eco130I CCWWGG 3 cut(s) 285, 788, 1098
Eco47I GGWCC 1 cut(s) 282
Eco57I CTGAAG 1 cut(s) 702
Eco88I CYCGRG 1 cut(s) 1032
EcoRII CCWGG 1 cut(s) 735
EcoT14I CCWWGG 3 cut(s) 285, 788, 1098
EcoT22I ATGCAT 1 cut(s) 150
ErhI CCWWGG 3 cut(s) 285, 788, 1098
FaeI CATG 6 cut(s) 148, 488, 599, 864, 872, 1145
FalI AAGNNNNNCTT 2 cut(s) 772, 804
FatI CATG 6 cut(s) 144, 484, 595, 860, 868, 1141
Fnu4HI GCNGC 1 cut(s) 795
FokI GGATG 6 cut(s) 53, 374, 414, 597, 909, 1105
Fsp4HI GCNGC 1 cut(s) 795
FspBI CTAG 3 cut(s) 432, 665, 879
GluI GCNGC 1 cut(s) 795
HaeIII GGCC 3 cut(s) 133, 294, 1097
Hin1II CATG 6 cut(s) 148, 488, 599, 864, 872, 1145
HindIII AAGCTT 2 cut(s) 355, 818
HinfI GANTC 4 cut(s) 140, 302, 656, 752
HphI GGTGA 2 cut(s) 182, 941
Hpy166II GTNNAC 4 cut(s) 6, 173, 589, 962
Hpy188I TCNGA 6 cut(s) 198, 279, 655, 682, 751, 770
Hpy188III TCNNGA 2 cut(s) 104, 351
Hpy8I GTNNAC 4 cut(s) 6, 173, 589, 962
HpyAV CCTTC 1 cut(s) 896
HpyCH4III ACNGT 1 cut(s) 184
HpyCH4IV ACGT 3 cut(s) 607, 845, 944
HpyF3I CTNAG 1 cut(s) 278
HpySE526I ACGT 3 cut(s) 607, 845, 944
Hsp92II CATG 6 cut(s) 148, 488, 599, 864, 872, 1145
KpnI GGTACC 1 cut(s) 878
Kzo9I GATC 3 cut(s) 97, 202, 689
Lsp1109I GCAGC 1 cut(s) 781
LweI GCATC 3 cut(s) 97, 356, 436
MaeI CTAG 3 cut(s) 432, 665, 879
MaeII ACGT 3 cut(s) 607, 845, 944
MaeIII GTNAC 4 cut(s) 496, 670, 863, 1084
MalI GATC 3 cut(s) 99, 204, 691
MboI GATC 3 cut(s) 97, 202, 689
MboII GAAGA 4 cut(s) 92, 107, 264, 695
MfeI CAATTG 1 cut(s) 471
MflI RGATCY 3 cut(s) 97, 202, 689
MluCI AATT 8 cut(s) 27, 35, 46, 366, 375, 404, 471, 1136
MnlI CCTC 6 cut(s) 85, 233, 273, 428, 797, 865
Mph1103I ATGCAT 1 cut(s) 150
MroXI GAANNNNTTC 2 cut(s) 99, 966
MseI TTAA 3 cut(s) 15, 72, 317
MslI CAYNNNNRTG 2 cut(s) 168, 596
MspR9I CCNGG 1 cut(s) 737
MunI CAATTG 1 cut(s) 471
MvaI CCWGG 1 cut(s) 737
NdeII GATC 3 cut(s) 97, 202, 689
NlaIII CATG 6 cut(s) 148, 488, 599, 864, 872, 1145
NlaIV GGNNCC 3 cut(s) 572, 876, 1027
NmuCI GTSAC 2 cut(s) 496, 863
NsiI ATGCAT 1 cut(s) 150
NspI RCATGY 1 cut(s) 864
PciI ACATGT 1 cut(s) 860
PdmI GAANNNNTTC 2 cut(s) 99, 966
PfeI GAWTC 4 cut(s) 140, 302, 656, 752
PflMI CCANNNNNTGG 1 cut(s) 581
PkrI GCNGC 1 cut(s) 796
PscI ACATGT 1 cut(s) 860
Psp1406I AACGTT 1 cut(s) 944
Psp6I CCWGG 1 cut(s) 735
PspGI CCWGG 1 cut(s) 735
PspN4I GGNNCC 3 cut(s) 572, 876, 1027
PspPI GGNCC 2 cut(s) 131, 282
PstI CTGCAG 2 cut(s) 194, 629
PsuI RGATCY 3 cut(s) 97, 202, 689
RsaI GTAC 5 cut(s) 5, 328, 603, 876, 1114
RsaNI GTAC 5 cut(s) 4, 327, 602, 875, 1113
RseI CAYNNNNRTG 2 cut(s) 168, 596
SaqAI TTAA 3 cut(s) 15, 72, 317
SatI GCNGC 1 cut(s) 795
Sau3AI GATC 3 cut(s) 97, 202, 689
Sau96I GGNCC 2 cut(s) 131, 282
ScaI AGTACT 2 cut(s) 328, 1114
ScrFI CCNGG 1 cut(s) 737
SfaNI GCATC 3 cut(s) 97, 356, 436
SfcI CTRYAG 2 cut(s) 190, 625
SinI GGWCC 1 cut(s) 282
SmiMI CAYNNNNRTG 2 cut(s) 168, 596
Sse9I AATT 8 cut(s) 27, 35, 46, 366, 375, 404, 471, 1136
SsiI CCGC 2 cut(s) 894, 1029
SspMI CTAG 3 cut(s) 432, 665, 879
StyD4I CCNGG 1 cut(s) 735
StyI CCWWGG 3 cut(s) 285, 788, 1098
TaaI ACNGT 1 cut(s) 184
TaiI ACGT 3 cut(s) 610, 848, 947
TaqI TCGA 3 cut(s) 81, 87, 907
TasI AATT 8 cut(s) 27, 35, 46, 366, 375, 404, 471, 1136
TatI WGTACW 3 cut(s) 3, 326, 1112
TfiI GAWTC 4 cut(s) 140, 302, 656, 752
Tru1I TTAA 3 cut(s) 15, 72, 317
Tru9I TTAA 3 cut(s) 15, 72, 317
TscAI CASTG 2 cut(s) 213, 544
TseFI GTSAC 2 cut(s) 496, 863
TseI GCWGC 1 cut(s) 794
Tsp45I GTSAC 2 cut(s) 496, 863
TspDTI ATGAA 5 cut(s) 20, 29, 72, 584, 1149
TspRI CASTG 2 cut(s) 213, 544
Van91I CCANNNNNTGG 1 cut(s) 581
VpaK11BI GGWCC 1 cut(s) 282
XapI RAATTY 3 cut(s) 35, 375, 404
XceI RCATGY 1 cut(s) 864
XcmI CCANNNNNNNNNTGG 2 cut(s) 125, 786
XmnI GAANNNNTTC 2 cut(s) 99, 966
XspI CTAG 3 cut(s) 432, 665, 879
ZrmI AGTACT 2 cut(s) 328, 1114
Zsp2I ATGCAT 1 cut(s) 150
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.