Rroxscaffold_3G00248630

Angio-associated migratory cell

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0001404
Physical Location & Seq
Forward (+)
41994344 .. 41999642
5299 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00248630.1

Sequence Viewer

Length: 927 bp
ATGAGCAGTCCAGCTCATGCTCTTCATGAAGAAGAAGATGACCAAGGCGAGGTTTTTCTCGATGAAGCAGACATCGTTGGTGAAATTGCAGTCGACGAAGAAGATCTTCCTGATGCAGATGATGAAGTTGATGAGCTTGATGATTCCATACACATATTTACTGGTCATAGCGACGAACTGTACGCCGTTATATGCAGCCCGACAGATCCTACTTTGGTTGCAACAGGGGGTAAAGATGACAGAGGGTTTCTTTGGAAGATTGGACACGGAGATTGGGCTTTTGAGCTTCAAGGTCATAAGGATTCTGTTTCTAGCTTAGCATTTAGTACTGATGGACAGTTGCTAGCATCTGGAAGCTTGGATGGAATCATCCAAATTTGGGATATAGCATCAGGAAATCTAAAATCTACTTTAGAAGGTCCTGGCGGGGGAATTGAGTGGATCAAGTGGCATCCTAGAGGTCATGTGGTCTTGGCTGGTTCAGAAGACTCTGCAGTTTGGATGTGGAATGCTGACAATGGTTCTTATCTTAATTTATTTTCTGGTCATGGTGGTAGGGTGAGTTGTGGTGACTTTACCCCTGATGGAAAAACAATCTGCACTGGTTCTGCTGATACAACTTTGAGAATATGGAATCCAAAAAGTGGCGAGAGCATTCATATGGTACAAGGTCACCCATACCATACTACAGGATTGACATGTTTGACAATAAGTTCTGATTCAACTTTAGCTGTTACTGGTTCTGAAGATGGTTCTGTTTATATTGTAAACATAACAACTGGAAAGGTTGTTAGTTCCCTGGTTTCACATTTAGATTCAGTTGAATGTATCGGGCTTGCTCCAAGGTACAGTCATCTCTCGTTCTCTCTTGCTCTCTCTCTCTCTCTCTCTCTTTCTCTGTTTGTGTGTGAGAGAGAGAGAGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

308

Amino Acids

32.98

Weight (kDa)

4.37

Isoelectric Point (pI)

33.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_SCAP PF24017 45 - 132 3.6e-06 SCAP Beta-propeller
Beta-prop_WDR5 PF25175 49 - 138 4.4e-17 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 49 - 212 2.1e-30 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 49 - 211 3e-20 CDC20/Fizzy WD40 domain
Beta-prop_WDR36-Utp21_1st PF25171 50 - 172 4.3e-09 WDR36/Utp21 first beta-propeller
Beta-prop_TEP1_2nd PF25047 51 - 133 2.3e-06 TEP-1 second beta-propeller
Beta-prop_THOC3 PF25174 52 - 288 2e-36 THOC3 beta-propeller domain
Beta-prop_EML PF23409 53 - 172 3.9e-08 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR75_1st PF23869 55 - 158 1.4e-07 WD repeat-containing protein 75 first beta-propeller
WDR55 PF24796 56 - 211 1.7e-11 WDR55
NBCH_WD40 PF20426 58 - 133 6.2e-07 Neurobeachin beta propeller domain
Beta-prop_EML_2 PF23414 60 - 220 4.9e-17 Echinoderm microtubule-associated protein second beta-propeller
WD40_Gbeta PF25391 61 - 168 2.5e-11 G protein beta WD-40 repeat protein
WD40_Prp19 PF24814 62 - 228 3.4e-26 Prp19 WD40 domain
WD40_WDHD1_1st PF24817 64 - 138 5.6e-10 WDHD1 first WD40 domain
Beta-prop_WDR3_2nd PF25172 94 - 212 1.2e-18 WDR3 second beta-propeller domain
Beta-prop_TEP1_2nd PF25047 94 - 188 1.6e-09 TEP-1 second beta-propeller
EIF3I PF24805 95 - 177 2e-08 EIF3I
WD40 PF00400 95 - 128 4.6e-11 WD domain, G-beta repeat
Beta-prop_WDR5 PF25175 96 - 274 1.2e-42 WDR5 beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 96 - 153 8.1e-06 CAF1B/HIR1 beta-propeller domain
Beta-prop_Aladin PF25460 100 - 200 6.7e-09 Aladin seven-bladed propeller
Beta-prop_WDR19_1st PF23389 100 - 222 8e-08 WDR19 first beta-propeller
Beta-prop_WDR3_1st PF25173 126 - 282 5e-24 WDR3 first beta-propeller domain
EIF3I PF24805 138 - 223 1.2e-12 EIF3I
Beta-prop_CAF1B_HIR1 PF24105 145 - 223 5.7e-07 CAF1B/HIR1 beta-propeller domain
Beta-prop_Aladin PF25460 146 - 215 4.6e-06 Aladin seven-bladed propeller
WD40_Gbeta PF25391 147 - 212 1.1e-08 G protein beta WD-40 repeat protein
WDR55 PF24796 148 - 286 3.8e-09 WDR55
WD40_WDHD1_1st PF24817 148 - 226 1.1e-08 WDHD1 first WD40 domain
WD40 PF00400 176 - 212 1.8e-08 WD domain, G-beta repeat
Beta-prop_TEP1_2nd PF25047 180 - 277 1.3e-09 TEP-1 second beta-propeller
Beta-prop_EML_2 PF23414 193 - 287 2.7e-08 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 199 - 277 1.3e-06 WDR36/Utp21 first beta-propeller
WD40 PF00400 217 - 255 9.2e-06 WD domain, G-beta repeat
Beta-prop_WDR3_2nd PF25172 228 - 290 5.7e-06 WDR3 second beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 644
AccI GTMKAC 1 cut(s) 93
AciI CCGC 1 cut(s) 426
AclWI GGATC 2 cut(s) 200, 449
AcsI RAATTY 1 cut(s) 375
AcuI CTGAAG 1 cut(s) 765
AfaI GTAC 4 cut(s) 182, 328, 666, 848
AfiI CCNNNNNNNGG 4 cut(s) 49, 379, 428, 644
AflIII ACRYGT 1 cut(s) 698
AgsI TTSAA 3 cut(s) 290, 723, 824
AjnI CCWGG 2 cut(s) 421, 798
AluBI AGCT 6 cut(s) 14, 136, 286, 315, 357, 731
AluI AGCT 6 cut(s) 14, 136, 286, 315, 357, 731
Alw26I GTCTC 1 cut(s) 915
AlwI GGATC 2 cut(s) 200, 449
ApeKI GCWGC 1 cut(s) 195
ApoI RAATTY 1 cut(s) 375
Asp700I GAANNNNTTC 1 cut(s) 105
AspS9I GGNCC 1 cut(s) 419
AsuHPI GGTGA 4 cut(s) 92, 571, 581, 665
AsuNHI GCTAGC 1 cut(s) 343
AvaII GGWCC 1 cut(s) 419
BbsI GAAGAC 1 cut(s) 492
BbvI GCAGC 1 cut(s) 207
BccI CCATC 4 cut(s) 326, 356, 578, 743
BceAI ACGGC 1 cut(s) 170
BciT130I CCWGG 2 cut(s) 423, 800
BcoDI GTCTC 1 cut(s) 915
BfaI CTAG 3 cut(s) 312, 344, 456
BfmI CTRYAG 2 cut(s) 492, 687
BglII AGATCT 1 cut(s) 103
BisI GCNGC 1 cut(s) 196
BlpI GCTNAGC 1 cut(s) 316
BlsI GCNGC 1 cut(s) 197
BmcAI AGTACT 1 cut(s) 328
Bme1390I CCNGG 2 cut(s) 423, 800
Bme18I GGWCC 1 cut(s) 419
BmgT120I GGNCC 1 cut(s) 419
BmrFI CCNGG 2 cut(s) 423, 800
BmsI GCATC 4 cut(s) 103, 356, 398, 460
BmtI GCTAGC 1 cut(s) 347
BpiI GAAGAC 1 cut(s) 492
Bpu1102I GCTNAGC 1 cut(s) 316
BsaJI CCNNGG 3 cut(s) 43, 798, 842
Bsc4I CCNNNNNNNGG 4 cut(s) 49, 379, 428, 644
Bse1I ACTGG 4 cut(s) 166, 607, 742, 784
BseBI CCWGG 2 cut(s) 423, 800
BseDI CCNNGG 3 cut(s) 43, 798, 842
BseGI GGATG 4 cut(s) 367, 369, 451, 507
BseLI CCNNNNNNNGG 4 cut(s) 49, 379, 428, 644
BseNI ACTGG 4 cut(s) 166, 607, 742, 784
BseXI GCAGC 1 cut(s) 207
BsgI GTGCAG 1 cut(s) 583
BslI CCNNNNNNNGG 4 cut(s) 49, 379, 428, 644
BsmAI GTCTC 1 cut(s) 915
BsmI GAATGC 2 cut(s) 514, 654
Bsp143I GATC 3 cut(s) 103, 205, 441
Bsp1720I GCTNAGC 1 cut(s) 316
BspACI CCGC 1 cut(s) 426
BspHI TCATGA 1 cut(s) 25
BspMAI CTGCAG 1 cut(s) 496
BspOI GCTAGC 1 cut(s) 347
BspPI GGATC 2 cut(s) 200, 449
BspQI GCTCTTC 1 cut(s) 27
BsrI ACTGG 4 cut(s) 166, 607, 742, 784
BssECI CCNNGG 3 cut(s) 43, 798, 842
BssMI GATC 3 cut(s) 103, 205, 441
BssT1I CCWWGG 2 cut(s) 43, 842
Bst2UI CCWGG 2 cut(s) 423, 800
Bst4CI ACNGT 3 cut(s) 180, 339, 851
Bst6I CTCTTC 1 cut(s) 27
BstC8I GCNNGC 2 cut(s) 345, 837
BstDEI CTNAG 1 cut(s) 316
BstEII GGTNACC 1 cut(s) 671
BstF5I GGATG 4 cut(s) 367, 369, 451, 507
BstKTI GATC 3 cut(s) 106, 208, 444
BstMAI GTCTC 1 cut(s) 915
BstMBI GATC 3 cut(s) 103, 205, 441
BstNI CCWGG 2 cut(s) 423, 800
BstNSI RCATGY 1 cut(s) 702
BstPI GGTNACC 1 cut(s) 671
BstSCI CCNGG 2 cut(s) 421, 798
BstSFI CTRYAG 2 cut(s) 492, 687
BstV1I GCAGC 1 cut(s) 207
BstV2I GAAGAC 1 cut(s) 492
BstX2I RGATCY 2 cut(s) 103, 205
BstYI RGATCY 2 cut(s) 103, 205
BtsCI GGATG 4 cut(s) 367, 369, 451, 507
BtsIMutI CAGTG 1 cut(s) 600
Cac8I GCNNGC 2 cut(s) 345, 837
CciI TCATGA 1 cut(s) 25
Cfr13I GGNCC 1 cut(s) 419
Csp6I GTAC 4 cut(s) 181, 327, 665, 847
CviAII CATG 5 cut(s) 17, 26, 464, 548, 699
CviQI GTAC 4 cut(s) 181, 327, 665, 847
DdeI CTNAG 1 cut(s) 316
DpnI GATC 3 cut(s) 105, 207, 443
DpnII GATC 3 cut(s) 103, 205, 441
Eam1104I CTCTTC 1 cut(s) 27
EarI CTCTTC 1 cut(s) 27
Eco130I CCWWGG 2 cut(s) 43, 842
Eco47I GGWCC 1 cut(s) 419
Eco57I CTGAAG 1 cut(s) 765
Eco91I GGTNACC 1 cut(s) 671
EcoO109I RGGNCCY 1 cut(s) 419
EcoO65I GGTNACC 1 cut(s) 671
EcoRII CCWGG 2 cut(s) 421, 798
EcoT14I CCWWGG 2 cut(s) 43, 842
ErhI CCWWGG 2 cut(s) 43, 842
FaeI CATG 5 cut(s) 20, 29, 467, 551, 702
FalI AAGNNNNNCTT 2 cut(s) 90, 122
FatI CATG 5 cut(s) 16, 25, 463, 547, 698
FauI CCCGC 1 cut(s) 419
FauNDI CATATG 1 cut(s) 660
FblI GTMKAC 1 cut(s) 93
Fnu4HI GCNGC 1 cut(s) 196
FokI GGATG 4 cut(s) 356, 374, 438, 514
Fsp4HI GCNGC 1 cut(s) 196
FspBI CTAG 3 cut(s) 312, 344, 456
GluI GCNGC 1 cut(s) 196
Hin1II CATG 5 cut(s) 20, 29, 467, 551, 702
HincII GTYRAC 1 cut(s) 94
HindII GTYRAC 1 cut(s) 94
HindIII AAGCTT 1 cut(s) 355
HinfI GANTC 7 cut(s) 143, 302, 366, 488, 634, 719, 815
HphI GGTGA 4 cut(s) 92, 571, 581, 665
Hpy166II GTNNAC 2 cut(s) 94, 769
Hpy188I TCNGA 3 cut(s) 484, 718, 745
Hpy188III TCNNGA 5 cut(s) 26, 59, 110, 351, 393
Hpy8I GTNNAC 2 cut(s) 94, 769
Hpy99I CGWCG 2 cut(s) 98, 176
HpyAV CCTTC 1 cut(s) 410
HpyCH4III ACNGT 3 cut(s) 180, 339, 851
HpyCH4V TGCA 6 cut(s) 89, 116, 195, 221, 494, 600
HpyF3I CTNAG 1 cut(s) 316
Hsp92II CATG 5 cut(s) 20, 29, 467, 551, 702
Kzo9I GATC 3 cut(s) 103, 205, 441
LguI GCTCTTC 1 cut(s) 27
LmnI GCTCC 1 cut(s) 844
Lsp1109I GCAGC 1 cut(s) 207
LweI GCATC 4 cut(s) 103, 356, 398, 460
MaeI CTAG 3 cut(s) 312, 344, 456
MaeIII GTNAC 3 cut(s) 569, 671, 733
MalI GATC 3 cut(s) 105, 207, 443
MboI GATC 3 cut(s) 103, 205, 441
MflI RGATCY 2 cut(s) 103, 205
MluCI AATT 4 cut(s) 84, 375, 432, 532
MlyI GAGTC 1 cut(s) 482
MnlI CCTC 3 cut(s) 43, 236, 452
MroXI GAANNNNTTC 1 cut(s) 105
MseI TTAA 1 cut(s) 531
MslI CAYNNNNRTG 1 cut(s) 659
MspR9I CCNGG 2 cut(s) 423, 800
Mva1269I GAATGC 2 cut(s) 514, 654
MvaI CCWGG 2 cut(s) 423, 800
NdeI CATATG 1 cut(s) 660
NdeII GATC 3 cut(s) 103, 205, 441
NheI GCTAGC 1 cut(s) 343
NlaIII CATG 5 cut(s) 20, 29, 467, 551, 702
NmuCI GTSAC 2 cut(s) 569, 671
NspI RCATGY 1 cut(s) 702
PagI TCATGA 1 cut(s) 25
PciI ACATGT 1 cut(s) 698
PciSI GCTCTTC 1 cut(s) 27
PctI GAATGC 2 cut(s) 514, 654
PdmI GAANNNNTTC 1 cut(s) 105
PfeI GAWTC 6 cut(s) 143, 302, 366, 634, 719, 815
PflMI CCANNNNNTGG 1 cut(s) 644
PkrI GCNGC 1 cut(s) 197
PleI GAGTC 1 cut(s) 482
PpsI GAGTC 1 cut(s) 482
PpuMI RGGWCCY 1 cut(s) 419
PscI ACATGT 1 cut(s) 698
Psp5II RGGWCCY 1 cut(s) 419
Psp6I CCWGG 2 cut(s) 421, 798
PspEI GGTNACC 1 cut(s) 671
PspGI CCWGG 2 cut(s) 421, 798
PspPI GGNCC 1 cut(s) 419
PspPPI RGGWCCY 1 cut(s) 419
PstI CTGCAG 1 cut(s) 496
PsuI RGATCY 2 cut(s) 103, 205
RsaI GTAC 4 cut(s) 182, 328, 666, 848
RsaNI GTAC 4 cut(s) 181, 327, 665, 847
RseI CAYNNNNRTG 1 cut(s) 659
SalI GTCGAC 1 cut(s) 92
SapI GCTCTTC 1 cut(s) 27
SaqAI TTAA 1 cut(s) 531
SatI GCNGC 1 cut(s) 196
Sau3AI GATC 3 cut(s) 103, 205, 441
Sau96I GGNCC 1 cut(s) 419
ScaI AGTACT 1 cut(s) 328
SchI GAGTC 1 cut(s) 482
ScrFI CCNGG 2 cut(s) 423, 800
SfaNI GCATC 4 cut(s) 103, 356, 398, 460
SfcI CTRYAG 2 cut(s) 492, 687
SinI GGWCC 1 cut(s) 419
SmiMI CAYNNNNRTG 1 cut(s) 659
Sse9I AATT 4 cut(s) 84, 375, 432, 532
SsiI CCGC 1 cut(s) 426
SspMI CTAG 3 cut(s) 312, 344, 456
StyD4I CCNGG 2 cut(s) 421, 798
StyI CCWWGG 2 cut(s) 43, 842
TaaI ACNGT 3 cut(s) 180, 339, 851
TaqI TCGA 2 cut(s) 60, 93
TasI AATT 4 cut(s) 84, 375, 432, 532
TatI WGTACW 1 cut(s) 326
TfiI GAWTC 6 cut(s) 143, 302, 366, 634, 719, 815
Tru1I TTAA 1 cut(s) 531
Tru9I TTAA 1 cut(s) 531
TscAI CASTG 1 cut(s) 607
TseFI GTSAC 2 cut(s) 569, 671
TseI GCWGC 1 cut(s) 195
Tsp45I GTSAC 2 cut(s) 569, 671
TspDTI ATGAA 5 cut(s) 14, 42, 78, 138, 647
TspGWI ACGGA 1 cut(s) 282
TspRI CASTG 1 cut(s) 607
Van91I CCANNNNNTGG 1 cut(s) 644
VpaK11BI GGWCC 1 cut(s) 419
XapI RAATTY 1 cut(s) 375
XceI RCATGY 1 cut(s) 702
XmiI GTMKAC 1 cut(s) 93
XmnI GAANNNNTTC 1 cut(s) 105
XspI CTAG 3 cut(s) 312, 344, 456
ZrmI AGTACT 1 cut(s) 328
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.