Rh5CG229800

Angio-associated migratory cell

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0001404
Physical Location & Seq
Reverse (-)
23734617 .. 23755107
20491 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG229800.1

Sequence Viewer

Length: 897 bp
ATGACTGTAACTTACACAGATGGGCCTGATGATTCCATGCATACTTTTACTGGTCATACAGGTGAACTTTATACAGTTGTCTGCAGTCCGACAGATCCAGTGTTAGTTGCAACAGGGGGTGGAGATGACAGAGGGTTTCTTTGGAAGATTGGACAAGGAGATTGGGCTTCTGAGGTCCAAGGCCATAAGGATTCTGTTTCTAGTTTAGCATTTAGTACTGATGGACAGTTGCTTGCATCTGGAAGCTTGGATGGAATTATTCAAATTTGGGATATAACATCAGCAAATCTAAAAAGCACTCTTGAAGGTCCTGGAGGTAAATTCTTATGCAGTATTTCTTACTGTTGCTGTACTGTAGATTATCTGAAATTTATTCTGGACCATGTGACTTCTCAGTGGGTCAGGTGGCATCCTAGAGGGTATCTGGTCTTGGCTGGTTCGTTCCTATCTTCAATTGTTTCAGGGCATGGTGGTAGCGTGACTTGTGGTGATTTTACCCCCGATGGTCATCCTTACCATTCTGCAGGGTTGACATGCTTGGCAATAAGCTCTGATTCAACTCTAGCTGTTACTGGTTCTGAAGATGGATCTGTCCATATTGTAAATATAATTACTGGAAAGGATGGAGTGACATGCTTGACCTGGCTTGGGGTATCTAGGTACCTAGCAACAGGTTGTGCGGATGGGAAGGTCCGATTATGGGATAGTCTCTCTGGTGATTGTGTAAGAACCTTCAATGGGCATAGTGAACCCATTCAGTCTCTCTCTGTATCTGCCAACAAGGACTTCCTTGTTTCAGTATCTACTAATGGAACCGCCCGAGTTTTTGAGATTGAAAAATATAAGCAAGCAATACCCTTCAGAGTTGGTAACAACGATGGCTTTGGAGTTATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

298

Amino Acids

31.62

Weight (kDa)

5.1

Isoelectric Point (pI)

27.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_THOC3 PF25174 11 - 100 2.7e-15 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 12 - 103 5.9e-17 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 12 - 103 3.7e-14 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 12 - 106 2.7e-11 Prp19 WD40 domain
Beta-prop_SCAP PF24017 13 - 94 1.3e-06 SCAP Beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 13 - 103 4.1e-06 WDR36/Utp21 first beta-propeller
Beta-prop_TEP1_2nd PF25047 15 - 95 3.6e-06 TEP-1 second beta-propeller
WD40_Gbeta PF25391 15 - 103 1.2e-08 G protein beta WD-40 repeat protein
WD40_WDHD1_1st PF24817 17 - 102 1.9e-09 WDHD1 first WD40 domain
NBCH_WD40 PF20426 23 - 96 2.1e-07 Neurobeachin beta propeller domain
Beta-prop_WDR3_2nd PF25172 30 - 107 6.7e-06 WDR3 second beta-propeller domain
WD40_CDC20-Fz PF24807 57 - 99 7.8e-07 CDC20/Fizzy WD40 domain
WD40 PF00400 59 - 91 1.7e-10 WD domain, G-beta repeat
WD40_Gbeta PF25391 151 - 285 1.4e-07 G protein beta WD-40 repeat protein
Beta-prop_TEP1_2nd PF25047 151 - 278 2.2e-10 TEP-1 second beta-propeller
WD40_Prp19 PF24814 170 - 280 1.3e-12 Prp19 WD40 domain
WDR55 PF24796 172 - 282 2.9e-11 WDR55
Beta-prop_WDR5 PF25175 173 - 281 2.5e-18 WDR5 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 174 - 277 3e-12 WDR3 second beta-propeller domain
WD40_WDHD1_1st PF24817 175 - 245 1.7e-06 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 176 - 283 9.5e-09 Echinoderm microtubule-associated protein second beta-propeller
WD40_CDC20-Fz PF24807 179 - 282 8.4e-12 CDC20/Fizzy WD40 domain
WD40_MABP1-WDR62_2nd PF24782 185 - 273 2.1e-08 MABP1/WDR62 second WD40 domain
EIF3I PF24805 201 - 284 5.3e-08 EIF3I
WD40 PF00400 203 - 235 8.6e-07 WD domain, G-beta repeat
Beta-prop_WDR3_1st PF25173 207 - 282 2.2e-12 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 208 - 285 1e-12 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 210 - 283 2.5e-10 WDHD1 first WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 219 - 281 7.2e-06 WDR36/Utp21 second beta-propeller domain
WD40 PF00400 239 - 276 1.6e-06 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 660
AccB1I GGYRCC 1 cut(s) 660
AciI CCGC 2 cut(s) 680, 816
AclWI GGATC 2 cut(s) 89, 595
AcsI RAATTY 3 cut(s) 264, 320, 368
AcuI CTGAAG 2 cut(s) 600, 844
AfaI GTAC 3 cut(s) 217, 352, 662
AfiI CCNNNNNNNGG 3 cut(s) 648, 700, 738
AgsI TTSAA 6 cut(s) 263, 305, 453, 558, 736, 836
AjnI CCWGG 2 cut(s) 310, 641
AjuI GAANNNNNNNTTGG 2 cut(s) 770, 802
AluBI AGCT 3 cut(s) 246, 549, 566
AluI AGCT 3 cut(s) 246, 549, 566
Alw26I GTCTC 2 cut(s) 713, 765
AlwI GGATC 2 cut(s) 89, 595
Ama87I CYCGRG 1 cut(s) 819
AoxI GGCC 2 cut(s) 23, 181
ApoI RAATTY 3 cut(s) 264, 320, 368
Asp700I GAANNNNTTC 1 cut(s) 753
Asp718I GGTACC 1 cut(s) 660
AspS9I GGNCC 5 cut(s) 23, 175, 308, 379, 691
AsuHPI GGTGA 3 cut(s) 74, 500, 728
AvaI CYCGRG 1 cut(s) 819
AvaII GGWCC 4 cut(s) 175, 308, 379, 691
BanI GGYRCC 1 cut(s) 660
BccI CCATC 8 cut(s) 14, 215, 245, 497, 578, 617, 677, 872
BciT130I CCWGG 2 cut(s) 312, 643
BcoDI GTCTC 2 cut(s) 713, 765
BfaI CTAG 5 cut(s) 201, 414, 563, 657, 665
BfmI CTRYAG 3 cut(s) 82, 354, 522
BmcAI AGTACT 1 cut(s) 217
Bme1390I CCNGG 2 cut(s) 312, 643
Bme18I GGWCC 4 cut(s) 175, 308, 379, 691
BmeT110I CYCGRG 1 cut(s) 819
BmgT120I GGNCC 5 cut(s) 23, 175, 308, 379, 691
BmiI GGNNCC 2 cut(s) 662, 814
BmrFI CCNGG 2 cut(s) 312, 643
BmsI GCATC 2 cut(s) 245, 418
BpmI CTGGAG 1 cut(s) 333
BsaBI GATNNNNATC 1 cut(s) 507
BsaJI CCNNGG 1 cut(s) 178
Bsc4I CCNNNNNNNGG 3 cut(s) 648, 700, 738
Bse1I ACTGG 4 cut(s) 55, 98, 577, 619
Bse8I GATNNNNATC 1 cut(s) 507
BseBI CCWGG 2 cut(s) 312, 643
BseDI CCNNGG 1 cut(s) 178
BseGI GGATG 5 cut(s) 256, 409, 508, 628, 688
BseJI GATNNNNATC 1 cut(s) 507
BseLI CCNNNNNNNGG 3 cut(s) 648, 700, 738
BseMII CTCAG 2 cut(s) 162, 407
BseNI ACTGG 4 cut(s) 55, 98, 577, 619
BshFI GGCC 2 cut(s) 25, 183
BshNI GGYRCC 1 cut(s) 660
BsiHKCI CYCGRG 1 cut(s) 819
BslI CCNNNNNNNGG 3 cut(s) 648, 700, 738
BsmAI GTCTC 2 cut(s) 713, 765
BsnI GGCC 2 cut(s) 25, 183
BsoBI CYCGRG 1 cut(s) 819
Bsp143I GATC 2 cut(s) 94, 587
BspACI CCGC 2 cut(s) 680, 816
BspANI GGCC 2 cut(s) 25, 183
BspCNI CTCAG 2 cut(s) 163, 406
BspLI GGNNCC 2 cut(s) 662, 814
BspMAI CTGCAG 2 cut(s) 86, 526
BspPI GGATC 2 cut(s) 89, 595
BspT107I GGYRCC 1 cut(s) 660
BsrI ACTGG 4 cut(s) 55, 98, 577, 619
BssECI CCNNGG 1 cut(s) 178
BssMI GATC 2 cut(s) 94, 587
BssT1I CCWWGG 1 cut(s) 178
Bst2UI CCWGG 2 cut(s) 312, 643
Bst4CI ACNGT 5 cut(s) 7, 76, 228, 344, 355
BstC8I GCNNGC 2 cut(s) 234, 849
BstDEI CTNAG 2 cut(s) 171, 393
BstF5I GGATG 5 cut(s) 256, 409, 508, 628, 688
BstKTI GATC 2 cut(s) 97, 590
BstMAI GTCTC 2 cut(s) 713, 765
BstMBI GATC 2 cut(s) 94, 587
BstNI CCWGG 2 cut(s) 312, 643
BstNSI RCATGY 2 cut(s) 537, 636
BstSCI CCNGG 2 cut(s) 310, 641
BstSFI CTRYAG 3 cut(s) 82, 354, 522
BstX2I RGATCY 2 cut(s) 94, 587
BstYI RGATCY 2 cut(s) 94, 587
BsuRI GGCC 2 cut(s) 25, 183
BtsCI GGATG 5 cut(s) 256, 409, 508, 628, 688
BtsIMutI CAGTG 2 cut(s) 105, 401
Cac8I GCNNGC 2 cut(s) 234, 849
Cfr13I GGNCC 5 cut(s) 23, 175, 308, 379, 691
Csp6I GTAC 3 cut(s) 216, 351, 661
CviAII CATG 5 cut(s) 37, 383, 467, 534, 633
CviJI RGCY 9 cut(s) 25, 167, 183, 246, 434, 549, 566, 646, 882
CviKI_1 RGCY 9 cut(s) 25, 167, 183, 246, 434, 549, 566, 646, 882
CviQI GTAC 3 cut(s) 216, 351, 661
DdeI CTNAG 2 cut(s) 171, 393
DpnI GATC 2 cut(s) 96, 589
DpnII GATC 2 cut(s) 94, 587
Eco130I CCWWGG 1 cut(s) 178
Eco47I GGWCC 4 cut(s) 175, 308, 379, 691
Eco57I CTGAAG 2 cut(s) 600, 844
Eco88I CYCGRG 1 cut(s) 819
EcoO109I RGGNCCY 1 cut(s) 308
EcoRII CCWGG 2 cut(s) 310, 641
EcoT14I CCWWGG 1 cut(s) 178
EcoT22I ATGCAT 1 cut(s) 42
ErhI CCWWGG 1 cut(s) 178
FaeI CATG 5 cut(s) 40, 386, 470, 537, 636
FatI CATG 5 cut(s) 36, 382, 466, 533, 632
FokI GGATG 5 cut(s) 263, 396, 495, 635, 695
FspBI CTAG 5 cut(s) 201, 414, 563, 657, 665
GsuI CTGGAG 1 cut(s) 333
HaeIII GGCC 2 cut(s) 25, 183
Hin1II CATG 5 cut(s) 40, 386, 470, 537, 636
HincII GTYRAC 1 cut(s) 531
HindII GTYRAC 1 cut(s) 531
HindIII AAGCTT 1 cut(s) 244
HinfI GANTC 3 cut(s) 32, 191, 554
HphI GGTGA 3 cut(s) 74, 500, 728
Hpy166II GTNNAC 3 cut(s) 65, 531, 749
Hpy188I TCNGA 7 cut(s) 90, 172, 366, 553, 580, 695, 863
Hpy188III TCNNGA 3 cut(s) 240, 302, 377
Hpy8I GTNNAC 3 cut(s) 65, 531, 749
HpyAV CCTTC 4 cut(s) 299, 682, 742, 868
HpyCH4III ACNGT 5 cut(s) 7, 76, 228, 344, 355
HpyCH4V TGCA 6 cut(s) 40, 84, 110, 236, 330, 524
HpyF3I CTNAG 2 cut(s) 171, 393
Hsp92II CATG 5 cut(s) 40, 386, 470, 537, 636
KpnI GGTACC 1 cut(s) 664
Kzo9I GATC 2 cut(s) 94, 587
LweI GCATC 2 cut(s) 245, 418
MaeI CTAG 5 cut(s) 201, 414, 563, 657, 665
MaeIII GTNAC 6 cut(s) 7, 385, 478, 568, 628, 869
MalI GATC 2 cut(s) 96, 589
MboI GATC 2 cut(s) 94, 587
MboII GAAGA 3 cut(s) 157, 441, 593
MfeI CAATTG 1 cut(s) 453
MflI RGATCY 2 cut(s) 94, 587
MluCI AATT 6 cut(s) 255, 264, 320, 368, 453, 609
MmeI TCCRAC 1 cut(s) 113
MnlI CCTC 4 cut(s) 125, 166, 308, 410
Mph1103I ATGCAT 1 cut(s) 42
MroXI GAANNNNTTC 1 cut(s) 753
MslI CAYNNNNRTG 1 cut(s) 60
MspR9I CCNGG 2 cut(s) 312, 643
MunI CAATTG 1 cut(s) 453
MvaI CCWGG 2 cut(s) 312, 643
NdeII GATC 2 cut(s) 94, 587
NlaIII CATG 5 cut(s) 40, 386, 470, 537, 636
NlaIV GGNNCC 2 cut(s) 662, 814
NmuCI GTSAC 3 cut(s) 385, 478, 628
NsiI ATGCAT 1 cut(s) 42
NspI RCATGY 2 cut(s) 537, 636
PdmI GAANNNNTTC 1 cut(s) 753
PfeI GAWTC 3 cut(s) 32, 191, 554
PfoI TCCNGGA 1 cut(s) 310
PpuMI RGGWCCY 1 cut(s) 308
Psp5II RGGWCCY 1 cut(s) 308
Psp6I CCWGG 2 cut(s) 310, 641
PspGI CCWGG 2 cut(s) 310, 641
PspN4I GGNNCC 2 cut(s) 662, 814
PspPI GGNCC 5 cut(s) 23, 175, 308, 379, 691
PspPPI RGGWCCY 1 cut(s) 308
PstI CTGCAG 2 cut(s) 86, 526
PsuI RGATCY 2 cut(s) 94, 587
RsaI GTAC 3 cut(s) 217, 352, 662
RsaNI GTAC 3 cut(s) 216, 351, 661
RseI CAYNNNNRTG 1 cut(s) 60
Sau3AI GATC 2 cut(s) 94, 587
Sau96I GGNCC 5 cut(s) 23, 175, 308, 379, 691
ScaI AGTACT 1 cut(s) 217
ScrFI CCNGG 2 cut(s) 312, 643
SfaNI GCATC 2 cut(s) 245, 418
SfcI CTRYAG 3 cut(s) 82, 354, 522
SinI GGWCC 4 cut(s) 175, 308, 379, 691
SmiMI CAYNNNNRTG 1 cut(s) 60
Sse9I AATT 6 cut(s) 255, 264, 320, 368, 453, 609
SsiI CCGC 2 cut(s) 680, 816
SspMI CTAG 5 cut(s) 201, 414, 563, 657, 665
StyD4I CCNGG 2 cut(s) 310, 641
StyI CCWWGG 1 cut(s) 178
TaaI ACNGT 5 cut(s) 7, 76, 228, 344, 355
TasI AATT 6 cut(s) 255, 264, 320, 368, 453, 609
TatI WGTACW 2 cut(s) 215, 350
TfiI GAWTC 3 cut(s) 32, 191, 554
TscAI CASTG 2 cut(s) 105, 401
TseFI GTSAC 3 cut(s) 385, 478, 628
Tsp45I GTSAC 3 cut(s) 385, 478, 628
TspRI CASTG 2 cut(s) 105, 401
VpaK11BI GGWCC 4 cut(s) 175, 308, 379, 691
XapI RAATTY 3 cut(s) 264, 320, 368
XceI RCATGY 2 cut(s) 537, 636
XmnI GAANNNNTTC 1 cut(s) 753
XspI CTAG 5 cut(s) 201, 414, 563, 657, 665
ZrmI AGTACT 1 cut(s) 217
Zsp2I ATGCAT 1 cut(s) 42
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.