Rw4G008120

Angio-associated migratory cell

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0001404
Physical Location & Seq
Forward (+)
17580571 .. 17584690
4120 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G008120.1

Sequence Viewer

Length: 1323 bp
ATGTACACGCTCTTTAAAACGTCTCTAATTCATAAATTTCATTGGAATTACATTGATGAAGCTGACATCCTTAACAAAGTCGATTTCGATGAGGAAGATCTTCCTGATACAGACGACCAAGTTGTTGATGGGCCTGATGATTCCATGCATACTTTTACTGGTCATACGGATGAACTTTATACAGTTGTCTGCAGTCCGACAGATCCAGTGTTAGTTGCAACAAGGGGTGGAGATGACAGAGGGTTTCTTTGGAAGATTGGACAAGGAGATTGGGCTTCTGAGGTCCAAGGCCATAAGGATTCTGTTTCAAGTTTAACATTTAGTACTGATGGACAGTTGCTTGCATCTGGAAGCTTGGATGGAATTATTCAAATTTGGGATATAACATCAGCAAATCTAAAATTTATTACTGTTGAAGGTCCTGGAGGGGGCATTGAGTGGGTCAGGTGGCATCCTAGAGGGTATCTGATCTTGGCTGGTTCGTTCCTATATTCAATTGTTTCAGGGCATGGTGGTAGCGTGACTTGTGGTGATTTTACCCCCGATGGTAAAACAATCTGCACTGGTTCTAATGTTGCAAGTTTGAGAATATGGAACCCAAAAAGTGGAGAGAACATTCATGTGGTACAACGTACGCTTTCAATTTACTTATTAAATTTTCTTTGTTCCTTGAAGTCATCCTTACCATTCTGCAGGTTGAAATGCTTGGCAATAAGCTCTGATTCAACTCTAGCTGTTACTGGTTCTGAAGATGGATCTGTCCATATTGTAAATATAACTACTGGAAAGGTTGTTAGTTCCCTGGCTTCACATTCAGATTCAGTAGAATGTATCGGACTTCCACCAAGTTTCCCTTGGGCTGCAACAGGAGGTATGGATAACAAGCTTATCATCTGGGATTTGCAACATACGTTGCCCCGTTCTACATGTGACCATGAGATTTTAAATCTCTGCAACTTGTCTAATGGAAGAAATCTTCCTTGTATGGCAGGATTGACATGCTTGACCTGGCTTGGGGTATCCAGGTACCTAGCAACAGGTTGTGCGGATGGGAAGGTCCGATTATGGGATAGTCTCTCTGGTGATTGTGTAAGAACCTTTAATGGGCATAGTGAACCCATTCAGTCTCTCTTTGTATTTGCCAACAAGGACTTCCTTGTTTCAGTATCTACTGATGGAACCGCCTGTGTTTTTGAGATTGAAAAATATAAGCAAGCAATATACCCTTCAGAGTTGGTAACAAGGATGGCCTTGGAGTTATATAGTACTTTTTTGTATAAACAATATGAATTACATGATTTTCTTATGGTACTCTTTTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

440

Amino Acids

48.34

Weight (kDa)

4.93

Isoelectric Point (pI)

38.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_THOC3 PF25174 47 - 157 4.8e-16 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 48 - 161 1.4e-16 WDR5 beta-propeller domain
WD40_Prp19 PF24814 48 - 160 9e-11 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 49 - 151 3.6e-12 WDR3 first beta-propeller domain
WD40_Gbeta PF25391 52 - 161 1.8e-08 G protein beta WD-40 repeat protein
WD40_WDHD1_1st PF24817 82 - 132 6.9e-07 WDHD1 first WD40 domain
NBCH_WD40 PF20426 82 - 131 9.1e-06 Neurobeachin beta propeller domain
WD40_CDC20-Fz PF24807 92 - 152 2.9e-07 CDC20/Fizzy WD40 domain
Beta-prop_EML_2 PF23414 94 - 207 7.2e-06 Echinoderm microtubule-associated protein second beta-propeller
WD40 PF00400 95 - 127 1e-09 WD domain, G-beta repeat
Beta-prop_TEP1_2nd PF25047 165 - 278 2e-06 TEP-1 second beta-propeller
WD40_CDC20-Fz PF24807 171 - 311 1.2e-09 CDC20/Fizzy WD40 domain
Beta-prop_WDR36-Utp21_1st PF25171 186 - 356 1.1e-07 WDR36/Utp21 first beta-propeller
NBCH_WD40 PF20426 231 - 304 6.7e-07 Neurobeachin beta propeller domain
Beta-prop_WDR3_1st PF25173 233 - 302 7.8e-09 WDR3 first beta-propeller domain
WD40_WDHD1_1st PF24817 233 - 307 2.7e-08 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 233 - 411 5.8e-11 Echinoderm microtubule-associated protein second beta-propeller
WD40_Prp19 PF24814 234 - 304 1.2e-07 Prp19 WD40 domain
Beta-prop_WDR3_2nd PF25172 234 - 304 2.5e-07 WDR3 second beta-propeller domain
EIF3I PF24805 235 - 405 1.8e-10 EIF3I
Beta-prop_WDR5 PF25175 235 - 303 1.8e-09 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 236 - 310 1.2e-07 THOC3 beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 262 - 405 1.1e-06 CAF1B/HIR1 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 266 - 401 3.4e-11 TEP-1 second beta-propeller
WD40_CDC20-Fz PF24807 313 - 403 6.2e-08 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_2nd PF25172 314 - 406 8.2e-07 WDR3 second beta-propeller domain
WD40_Prp19 PF24814 331 - 404 1.8e-08 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 331 - 405 2.7e-09 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 332 - 403 4.9e-12 WDR5 beta-propeller domain
WD40_WDHD1_1st PF24817 333 - 405 1e-08 WDHD1 first WD40 domain
Beta-prop_WDR3_1st PF25173 333 - 402 9.8e-11 WDR3 first beta-propeller domain
WD40_MABP1-WDR62_2nd PF24782 341 - 398 2e-08 MABP1/WDR62 second WD40 domain
WDR55 PF24796 342 - 407 5.4e-09 WDR55
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 684
Acc65I GGTACC 1 cut(s) 1026
AccB1I GGYRCC 1 cut(s) 1026
AccB7I CCANNNNNTGG 1 cut(s) 605
AciI CCGC 2 cut(s) 1046, 1182
AclWI GGATC 2 cut(s) 197, 763
AcsI RAATTY 4 cut(s) 35, 372, 401, 655
AcuI CTGAAG 2 cut(s) 768, 1212
AfaI GTAC 7 cut(s) 5, 325, 627, 634, 1028, 1267, 1311
AfiI CCNNNNNNNGG 5 cut(s) 428, 605, 1014, 1066, 1104
AflIII ACRYGT 1 cut(s) 926
AgsI TTSAA 9 cut(s) 309, 371, 416, 495, 642, 673, 700, 726, 1202
AjnI CCWGG 4 cut(s) 421, 801, 1007, 1022
AjuI GAANNNNNNNTTGG 2 cut(s) 1136, 1168
AluBI AGCT 5 cut(s) 62, 354, 717, 734, 886
AluI AGCT 5 cut(s) 62, 354, 717, 734, 886
Alw26I GTCTC 3 cut(s) 27, 1079, 1131
AlwI GGATC 2 cut(s) 197, 763
AoxI GGCC 3 cut(s) 131, 289, 1248
ApeKI GCWGC 1 cut(s) 860
ApoI RAATTY 4 cut(s) 35, 372, 401, 655
Asp700I GAANNNNTTC 2 cut(s) 99, 1119
Asp718I GGTACC 1 cut(s) 1026
AspS9I GGNCC 4 cut(s) 131, 283, 419, 1057
AsuHPI GGTGA 2 cut(s) 542, 1094
AvaII GGWCC 3 cut(s) 283, 419, 1057
BanI GGYRCC 1 cut(s) 1026
BbvI GCAGC 1 cut(s) 847
BccI CCATC 8 cut(s) 122, 323, 353, 539, 746, 1043, 1169, 1240
BciT130I CCWGG 4 cut(s) 423, 803, 1009, 1024
BciVI GTATCC 1 cut(s) 1030
BcoDI GTCTC 3 cut(s) 27, 1079, 1131
BfaI CTAG 3 cut(s) 456, 731, 1031
BfmI CTRYAG 2 cut(s) 190, 691
BfuAI ACCTGC 1 cut(s) 684
BfuI GTATCC 1 cut(s) 1030
BglII AGATCT 1 cut(s) 97
BisI GCNGC 1 cut(s) 861
BlsI GCNGC 1 cut(s) 862
BmcAI AGTACT 2 cut(s) 325, 1267
Bme1390I CCNGG 4 cut(s) 423, 803, 1009, 1024
Bme18I GGWCC 3 cut(s) 283, 419, 1057
BmgT120I GGNCC 4 cut(s) 131, 283, 419, 1057
BmiI GGNNCC 3 cut(s) 596, 1028, 1180
BmrFI CCNGG 4 cut(s) 423, 803, 1009, 1024
BmsI GCATC 2 cut(s) 353, 460
BpmI CTGGAG 1 cut(s) 444
BsaJI CCNNGG 4 cut(s) 286, 801, 854, 1251
Bsc4I CCNNNNNNNGG 5 cut(s) 428, 605, 1014, 1066, 1104
Bse1I ACTGG 5 cut(s) 163, 206, 568, 745, 787
BseBI CCWGG 4 cut(s) 423, 803, 1009, 1024
BseDI CCNNGG 4 cut(s) 286, 801, 854, 1251
BseGI GGATG 7 cut(s) 66, 175, 364, 451, 677, 1054, 1251
BseLI CCNNNNNNNGG 5 cut(s) 428, 605, 1014, 1066, 1104
BseMII CTCAG 1 cut(s) 270
BseNI ACTGG 5 cut(s) 163, 206, 568, 745, 787
BseXI GCAGC 1 cut(s) 847
BsgI GTGCAG 1 cut(s) 544
BshFI GGCC 3 cut(s) 133, 291, 1250
BshNI GGYRCC 1 cut(s) 1026
BsiWI CGTACG 1 cut(s) 632
BslI CCNNNNNNNGG 5 cut(s) 428, 605, 1014, 1066, 1104
BsmAI GTCTC 3 cut(s) 27, 1079, 1131
BsmBI CGTCTC 1 cut(s) 27
BsnI GGCC 3 cut(s) 133, 291, 1250
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 4 cut(s) 97, 202, 468, 755
BspACI CCGC 2 cut(s) 1046, 1182
BspANI GGCC 3 cut(s) 133, 291, 1250
BspCNI CTCAG 1 cut(s) 271
BspLI GGNNCC 3 cut(s) 596, 1028, 1180
BspMAI CTGCAG 2 cut(s) 194, 695
BspMI ACCTGC 1 cut(s) 684
BspPI GGATC 2 cut(s) 197, 763
BspT107I GGYRCC 1 cut(s) 1026
BsrGI TGTACA 1 cut(s) 3
BsrI ACTGG 5 cut(s) 163, 206, 568, 745, 787
BssECI CCNNGG 4 cut(s) 286, 801, 854, 1251
BssMI GATC 4 cut(s) 97, 202, 468, 755
BssT1I CCWWGG 3 cut(s) 286, 854, 1251
Bst2UI CCWGG 4 cut(s) 423, 803, 1009, 1024
Bst4CI ACNGT 3 cut(s) 184, 336, 412
BstAUI TGTACA 1 cut(s) 3
BstC8I GCNNGC 2 cut(s) 342, 1215
BstDEI CTNAG 1 cut(s) 279
BstF5I GGATG 7 cut(s) 66, 175, 364, 451, 677, 1054, 1251
BstKTI GATC 4 cut(s) 100, 205, 471, 758
BstMAI GTCTC 3 cut(s) 27, 1079, 1131
BstMBI GATC 4 cut(s) 97, 202, 468, 755
BstNI CCWGG 4 cut(s) 423, 803, 1009, 1024
BstNSI RCATGY 2 cut(s) 930, 1002
BstSCI CCNGG 4 cut(s) 421, 801, 1007, 1022
BstSFI CTRYAG 2 cut(s) 190, 691
BstV1I GCAGC 1 cut(s) 847
BstX2I RGATCY 3 cut(s) 97, 202, 755
BstYI RGATCY 3 cut(s) 97, 202, 755
BsuI GTATCC 1 cut(s) 1030
BsuRI GGCC 3 cut(s) 133, 291, 1250
BtsCI GGATG 7 cut(s) 66, 175, 364, 451, 677, 1054, 1251
BtsIMutI CAGTG 2 cut(s) 213, 561
BveI ACCTGC 1 cut(s) 684
Cac8I GCNNGC 2 cut(s) 342, 1215
Cfr13I GGNCC 4 cut(s) 131, 283, 419, 1057
Csp6I GTAC 7 cut(s) 4, 324, 626, 633, 1027, 1266, 1310
CviAII CATG 7 cut(s) 145, 509, 620, 927, 935, 999, 1295
CviQI GTAC 7 cut(s) 4, 324, 626, 633, 1027, 1266, 1310
DdeI CTNAG 1 cut(s) 279
DpnI GATC 4 cut(s) 99, 204, 470, 757
DpnII GATC 4 cut(s) 97, 202, 468, 755
DraI TTTAAA 2 cut(s) 16, 945
Eco130I CCWWGG 3 cut(s) 286, 854, 1251
Eco47I GGWCC 3 cut(s) 283, 419, 1057
Eco57I CTGAAG 2 cut(s) 768, 1212
EcoO109I RGGNCCY 1 cut(s) 419
EcoRII CCWGG 4 cut(s) 421, 801, 1007, 1022
EcoT14I CCWWGG 3 cut(s) 286, 854, 1251
EcoT22I ATGCAT 1 cut(s) 150
ErhI CCWWGG 3 cut(s) 286, 854, 1251
Esp3I CGTCTC 1 cut(s) 27
FaeI CATG 7 cut(s) 148, 512, 623, 930, 938, 1002, 1298
FalI AAGNNNNNCTT 4 cut(s) 665, 697, 838, 870
FatI CATG 7 cut(s) 144, 508, 619, 926, 934, 998, 1294
Fnu4HI GCNGC 1 cut(s) 861
FokI GGATG 7 cut(s) 53, 182, 371, 438, 664, 1061, 1258
Fsp4HI GCNGC 1 cut(s) 861
FspBI CTAG 3 cut(s) 456, 731, 1031
GluI GCNGC 1 cut(s) 861
GsuI CTGGAG 1 cut(s) 444
HaeIII GGCC 3 cut(s) 133, 291, 1250
Hin1II CATG 7 cut(s) 148, 512, 623, 930, 938, 1002, 1298
HindIII AAGCTT 2 cut(s) 352, 884
HinfI GANTC 4 cut(s) 140, 299, 722, 818
HphI GGTGA 2 cut(s) 542, 1094
Hpy166II GTNNAC 2 cut(s) 6, 1115
Hpy188I TCNGA 9 cut(s) 198, 280, 468, 721, 748, 817, 836, 1061, 1231
Hpy188III TCNNGA 2 cut(s) 104, 348
Hpy8I GTNNAC 2 cut(s) 6, 1115
HpyAV CCTTC 3 cut(s) 410, 1048, 1236
HpyCH4III ACNGT 3 cut(s) 184, 336, 412
HpyCH4IV ACGT 3 cut(s) 20, 631, 911
HpyF3I CTNAG 1 cut(s) 279
HpySE526I ACGT 3 cut(s) 20, 631, 911
Hsp92II CATG 7 cut(s) 148, 512, 623, 930, 938, 1002, 1298
KpnI GGTACC 1 cut(s) 1030
Kzo9I GATC 4 cut(s) 97, 202, 468, 755
Lsp1109I GCAGC 1 cut(s) 847
LweI GCATC 2 cut(s) 353, 460
MaeI CTAG 3 cut(s) 456, 731, 1031
MaeII ACGT 3 cut(s) 20, 631, 911
MaeIII GTNAC 4 cut(s) 520, 736, 929, 1237
MalI GATC 4 cut(s) 99, 204, 470, 757
MboI GATC 4 cut(s) 97, 202, 468, 755
MboII GAAGA 6 cut(s) 92, 107, 265, 761, 968, 981
MfeI CAATTG 1 cut(s) 495
MflI RGATCY 3 cut(s) 97, 202, 755
MmeI TCCRAC 1 cut(s) 221
MnlI CCTC 6 cut(s) 85, 233, 274, 419, 452, 863
Mph1103I ATGCAT 1 cut(s) 150
MroXI GAANNNNTTC 2 cut(s) 99, 1119
MseI TTAA 6 cut(s) 15, 72, 314, 653, 944, 1101
MslI CAYNNNNRTG 2 cut(s) 168, 620
MspR9I CCNGG 4 cut(s) 423, 803, 1009, 1024
MunI CAATTG 1 cut(s) 495
MvaI CCWGG 4 cut(s) 423, 803, 1009, 1024
NdeII GATC 4 cut(s) 97, 202, 468, 755
NlaIII CATG 7 cut(s) 148, 512, 623, 930, 938, 1002, 1298
NlaIV GGNNCC 3 cut(s) 596, 1028, 1180
NmuCI GTSAC 2 cut(s) 520, 929
NsiI ATGCAT 1 cut(s) 150
NspI RCATGY 2 cut(s) 930, 1002
PciI ACATGT 1 cut(s) 926
PdmI GAANNNNTTC 2 cut(s) 99, 1119
PfeI GAWTC 4 cut(s) 140, 299, 722, 818
Pfl23II CGTACG 1 cut(s) 632
PflMI CCANNNNNTGG 1 cut(s) 605
PfoI TCCNGGA 1 cut(s) 421
PkrI GCNGC 1 cut(s) 862
PpuMI RGGWCCY 1 cut(s) 419
PscI ACATGT 1 cut(s) 926
Psp5II RGGWCCY 1 cut(s) 419
Psp6I CCWGG 4 cut(s) 421, 801, 1007, 1022
PspGI CCWGG 4 cut(s) 421, 801, 1007, 1022
PspLI CGTACG 1 cut(s) 632
PspN4I GGNNCC 3 cut(s) 596, 1028, 1180
PspPI GGNCC 4 cut(s) 131, 283, 419, 1057
PspPPI RGGWCCY 1 cut(s) 419
PstI CTGCAG 2 cut(s) 194, 695
PsuI RGATCY 3 cut(s) 97, 202, 755
RsaI GTAC 7 cut(s) 5, 325, 627, 634, 1028, 1267, 1311
RsaNI GTAC 7 cut(s) 4, 324, 626, 633, 1027, 1266, 1310
RseI CAYNNNNRTG 2 cut(s) 168, 620
SaqAI TTAA 6 cut(s) 15, 72, 314, 653, 944, 1101
SatI GCNGC 1 cut(s) 861
Sau3AI GATC 4 cut(s) 97, 202, 468, 755
Sau96I GGNCC 4 cut(s) 131, 283, 419, 1057
ScaI AGTACT 2 cut(s) 325, 1267
ScrFI CCNGG 4 cut(s) 423, 803, 1009, 1024
SfaNI GCATC 2 cut(s) 353, 460
SfcI CTRYAG 2 cut(s) 190, 691
SinI GGWCC 3 cut(s) 283, 419, 1057
SmiMI CAYNNNNRTG 2 cut(s) 168, 620
SsiI CCGC 2 cut(s) 1046, 1182
SspMI CTAG 3 cut(s) 456, 731, 1031
StyD4I CCNGG 4 cut(s) 421, 801, 1007, 1022
StyI CCWWGG 3 cut(s) 286, 854, 1251
TaaI ACNGT 3 cut(s) 184, 336, 412
TaiI ACGT 3 cut(s) 23, 634, 914
TaqI TCGA 2 cut(s) 81, 87
TatI WGTACW 3 cut(s) 3, 323, 1265
TfiI GAWTC 4 cut(s) 140, 299, 722, 818
Tru1I TTAA 6 cut(s) 15, 72, 314, 653, 944, 1101
Tru9I TTAA 6 cut(s) 15, 72, 314, 653, 944, 1101
TscAI CASTG 2 cut(s) 213, 568
TseFI GTSAC 2 cut(s) 520, 929
TseI GCWGC 1 cut(s) 860
Tsp45I GTSAC 2 cut(s) 520, 929
TspDTI ATGAA 6 cut(s) 20, 29, 72, 186, 608, 1302
TspGWI ACGGA 1 cut(s) 182
TspRI CASTG 2 cut(s) 213, 568
Van91I CCANNNNNTGG 1 cut(s) 605
VpaK11BI GGWCC 3 cut(s) 283, 419, 1057
XapI RAATTY 4 cut(s) 35, 372, 401, 655
XceI RCATGY 2 cut(s) 930, 1002
XcmI CCANNNNNNNNNTGG 2 cut(s) 125, 852
XmnI GAANNNNTTC 2 cut(s) 99, 1119
XspI CTAG 3 cut(s) 456, 731, 1031
ZrmI AGTACT 2 cut(s) 325, 1267
Zsp2I ATGCAT 1 cut(s) 150
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.