RLG00000003067

Angio-associated migratory cell

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0001404
Physical Location & Seq
Forward (+)
43895772 .. 43903150
7379 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003067

Sequence Viewer

Length: 1077 bp
ATGAGCAGTCCAGCTCATGCTCTTCATGAAGAAGAAGATGACCAAGGCGAGGTTTTTCTCGATGAAGCAGACATTGTTGGTGAAATTGCAGTCAACGAAGAAGATCTTCCTGATGCAGACGATGAAGTTGATGAGCTTGATGATTCCATACACATATTTACTGGTCATAGCGACGAACTATACACCGTTATATGCAGCCCGACAGATCCTACTTTGGTTGCAACAGGGGGTAAAGATGACAGAGGGTTTCTTTGGAAGATTGGACACGGAGATTGGGCTTTTGAGCTTCAAGGTCATAAGGATTCTGTTTCTAGCTTAGCATTTAGTACTGATGGACAGTTGCTAGCATCTGGAAGCTTGGATGGAATCATCCAAATTTGGGATATAGCATCAGGAAATCTAAAATCTACTTTAGAAGGTCCTGGCGGGGGAATTGAGTGGATCAAGTGGCATCCTAGAGGTCATGTGGTTTTGGCTGGTTCAGAAGACTCTGCAGTTTGGATGTGGAATGCTGACAATGGTTCCTATCTTAATTTATTTTCTGGTCATGGTGGTAGGGTGAGTTGTGGTGACTTTACCCCTGATGGAAAAACAATCTGCACTGGTTCTGCTGATACAACTTTGAGAATATGGAATCCAAAAAGTGGCGAGAGCATTCATGTGGTACAAGGTCACTCATACCATACTACAGGATTGACATGTTTGACAATAAGTTCTGATTCAACTTTAGCTGTTACTGGTTCTGAAGATGGTTCTGTCTATATTGTAAACATAACAACTGGAAAGGTTGTTAGTTCCCTGGTTTCACATTTAGATTCAGTTGAATGTATCGGGCTTGCACCAAGCATTCATTGCCCCGTTGTACCTGCGAACATGAGGCACATGATCGAAAGAAACCCTATCATCATGAAGAGTGCAGGCGAGCCATTCTGTAGAAACTATCAGATAGAGGGAACTAGGCAACTAGCTATTCTGATATGGACGTATCCAGATATATATATATATATATATATATATATATATATATATATATATATTACATTTACAAAGCCTTGCTATATAATTCATTTTTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

359

Amino Acids

39.19

Weight (kDa)

4.54

Isoelectric Point (pI)

37.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR5 PF25175 49 - 138 4.6e-17 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 49 - 212 2.6e-30 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 49 - 211 5.2e-20 CDC20/Fizzy WD40 domain
Beta-prop_WDR36-Utp21_1st PF25171 50 - 172 6.5e-09 WDR36/Utp21 first beta-propeller
Beta-prop_TEP1_2nd PF25047 51 - 133 2.9e-06 TEP-1 second beta-propeller
Beta-prop_THOC3 PF25174 52 - 282 9.2e-36 THOC3 beta-propeller domain
Beta-prop_EML PF23409 54 - 172 1e-07 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR75_1st PF23869 55 - 158 1.9e-07 WD repeat-containing protein 75 first beta-propeller
WDR55 PF24796 56 - 242 6.1e-13 WDR55
NBCH_WD40 PF20426 59 - 133 1.8e-06 Neurobeachin beta propeller domain
WD40_Gbeta PF25391 61 - 168 2.9e-11 G protein beta WD-40 repeat protein
WD40_WDHD1_1st PF24817 62 - 138 3.9e-10 WDHD1 first WD40 domain
WD40_Prp19 PF24814 65 - 230 4.7e-26 Prp19 WD40 domain
Beta-prop_WDR3_2nd PF25172 94 - 212 1.6e-18 WDR3 second beta-propeller domain
Beta-prop_TEP1_2nd PF25047 94 - 188 2e-09 TEP-1 second beta-propeller
EIF3I PF24805 95 - 177 3.4e-08 EIF3I
WD40 PF00400 95 - 128 5.7e-11 WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1 PF24105 96 - 156 3.7e-06 CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR5 PF25175 96 - 270 2.1e-42 WDR5 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 98 - 223 3.8e-11 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR19_1st PF23389 100 - 223 1.3e-07 WDR19 first beta-propeller
Beta-prop_WDR3_1st PF25173 124 - 281 2.3e-24 WDR3 first beta-propeller domain
EIF3I PF24805 138 - 225 1.6e-12 EIF3I
Beta-prop_CAF1B_HIR1 PF24105 145 - 223 6.8e-07 CAF1B/HIR1 beta-propeller domain
Beta-prop_Aladin PF25460 146 - 215 4.9e-06 Aladin seven-bladed propeller
WDR55 PF24796 148 - 277 8.2e-09 WDR55
WD40_WDHD1_1st PF24817 148 - 224 2e-08 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 150 - 270 4.6e-12 Echinoderm microtubule-associated protein second beta-propeller
WD40_Gbeta PF25391 154 - 212 8.5e-08 G protein beta WD-40 repeat protein
WD40 PF00400 176 - 212 2.2e-08 WD domain, G-beta repeat
Beta-prop_TEP1_2nd PF25047 181 - 277 1.5e-09 TEP-1 second beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 199 - 277 1.8e-06 WDR36/Utp21 first beta-propeller
WD40 PF00400 216 - 255 6.7e-06 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 874
AccB7I CCANNNNNTGG 1 cut(s) 644
AciI CCGC 1 cut(s) 426
AclWI GGATC 2 cut(s) 200, 449
AcsI RAATTY 1 cut(s) 375
AcuI CTGAAG 1 cut(s) 765
AfaI GTAC 3 cut(s) 328, 666, 864
AfiI CCNNNNNNNGG 4 cut(s) 49, 379, 428, 644
AflIII ACRYGT 1 cut(s) 698
AgsI TTSAA 3 cut(s) 290, 723, 824
AjnI CCWGG 2 cut(s) 421, 798
AluBI AGCT 7 cut(s) 14, 136, 286, 315, 357, 731, 968
AluI AGCT 7 cut(s) 14, 136, 286, 315, 357, 731, 968
AlwI GGATC 2 cut(s) 200, 449
ApeKI GCWGC 1 cut(s) 195
ApoI RAATTY 1 cut(s) 375
Asp700I GAANNNNTTC 1 cut(s) 105
AspS9I GGNCC 1 cut(s) 419
AsuHPI GGTGA 3 cut(s) 92, 571, 581
AsuNHI GCTAGC 1 cut(s) 343
AvaII GGWCC 1 cut(s) 419
BbsI GAAGAC 1 cut(s) 492
BbvI GCAGC 1 cut(s) 207
BccI CCATC 4 cut(s) 326, 356, 578, 743
BciT130I CCWGG 2 cut(s) 423, 800
BciVI GTATCC 1 cut(s) 996
BfaI CTAG 5 cut(s) 312, 344, 456, 957, 965
BfmI CTRYAG 3 cut(s) 492, 687, 931
BfuAI ACCTGC 1 cut(s) 874
BfuI GTATCC 1 cut(s) 996
BglII AGATCT 1 cut(s) 103
BisI GCNGC 1 cut(s) 196
BlpI GCTNAGC 1 cut(s) 316
BlsI GCNGC 1 cut(s) 197
BmcAI AGTACT 1 cut(s) 328
Bme1390I CCNGG 2 cut(s) 423, 800
Bme18I GGWCC 1 cut(s) 419
BmgT120I GGNCC 1 cut(s) 419
BmiI GGNNCC 1 cut(s) 523
BmrFI CCNGG 2 cut(s) 423, 800
BmsI GCATC 4 cut(s) 103, 356, 398, 460
BmtI GCTAGC 1 cut(s) 347
BpiI GAAGAC 1 cut(s) 492
Bpu1102I GCTNAGC 1 cut(s) 316
BsaJI CCNNGG 2 cut(s) 43, 798
Bsc4I CCNNNNNNNGG 4 cut(s) 49, 379, 428, 644
Bse1I ACTGG 4 cut(s) 166, 607, 742, 784
Bse3DI GCAATG 1 cut(s) 850
BseBI CCWGG 2 cut(s) 423, 800
BseDI CCNNGG 2 cut(s) 43, 798
BseGI GGATG 4 cut(s) 367, 369, 451, 507
BseLI CCNNNNNNNGG 4 cut(s) 49, 379, 428, 644
BseMI GCAATG 1 cut(s) 850
BseNI ACTGG 4 cut(s) 166, 607, 742, 784
BseXI GCAGC 1 cut(s) 207
BsgI GTGCAG 2 cut(s) 583, 936
BslI CCNNNNNNNGG 4 cut(s) 49, 379, 428, 644
BsmI GAATGC 3 cut(s) 514, 654, 846
Bsp143I GATC 4 cut(s) 103, 205, 441, 885
Bsp1720I GCTNAGC 1 cut(s) 316
BspACI CCGC 1 cut(s) 426
BspHI TCATGA 2 cut(s) 25, 906
BspLI GGNNCC 1 cut(s) 523
BspMAI CTGCAG 1 cut(s) 496
BspMI ACCTGC 1 cut(s) 874
BspOI GCTAGC 1 cut(s) 347
BspPI GGATC 2 cut(s) 200, 449
BspQI GCTCTTC 1 cut(s) 27
BsrDI GCAATG 1 cut(s) 850
BsrI ACTGG 4 cut(s) 166, 607, 742, 784
BssECI CCNNGG 2 cut(s) 43, 798
BssMI GATC 4 cut(s) 103, 205, 441, 885
BssT1I CCWWGG 1 cut(s) 43
Bst2UI CCWGG 2 cut(s) 423, 800
Bst4CI ACNGT 2 cut(s) 187, 339
Bst6I CTCTTC 2 cut(s) 27, 905
BstAPI GCANNNNNTGC 1 cut(s) 852
BstC8I GCNNGC 4 cut(s) 345, 837, 919, 923
BstDEI CTNAG 1 cut(s) 316
BstF5I GGATG 4 cut(s) 367, 369, 451, 507
BstKTI GATC 4 cut(s) 106, 208, 444, 888
BstMBI GATC 4 cut(s) 103, 205, 441, 885
BstMWI GCNNNNNNNGC 1 cut(s) 852
BstNI CCWGG 2 cut(s) 423, 800
BstNSI RCATGY 1 cut(s) 702
BstSCI CCNGG 2 cut(s) 421, 798
BstSFI CTRYAG 3 cut(s) 492, 687, 931
BstV1I GCAGC 1 cut(s) 207
BstV2I GAAGAC 1 cut(s) 492
BstX2I RGATCY 2 cut(s) 103, 205
BstYI RGATCY 2 cut(s) 103, 205
BsuI GTATCC 1 cut(s) 996
BtsCI GGATG 4 cut(s) 367, 369, 451, 507
BtsIMutI CAGTG 1 cut(s) 600
BveI ACCTGC 1 cut(s) 874
Cac8I GCNNGC 4 cut(s) 345, 837, 919, 923
CciI TCATGA 2 cut(s) 25, 906
Cfr13I GGNCC 1 cut(s) 419
Csp6I GTAC 3 cut(s) 327, 665, 863
CviAII CATG 9 cut(s) 17, 26, 464, 548, 659, 699, 874, 883, 907
CviQI GTAC 3 cut(s) 327, 665, 863
DdeI CTNAG 1 cut(s) 316
DpnI GATC 4 cut(s) 105, 207, 443, 887
DpnII GATC 4 cut(s) 103, 205, 441, 885
Eam1104I CTCTTC 2 cut(s) 27, 905
EarI CTCTTC 2 cut(s) 27, 905
Eco130I CCWWGG 1 cut(s) 43
Eco47I GGWCC 1 cut(s) 419
Eco57I CTGAAG 1 cut(s) 765
EcoO109I RGGNCCY 1 cut(s) 419
EcoRII CCWGG 2 cut(s) 421, 798
EcoT14I CCWWGG 1 cut(s) 43
ErhI CCWWGG 1 cut(s) 43
FaeI CATG 9 cut(s) 20, 29, 467, 551, 662, 702, 877, 886, 910
FalI AAGNNNNNCTT 2 cut(s) 90, 122
FatI CATG 9 cut(s) 16, 25, 463, 547, 658, 698, 873, 882, 906
FauI CCCGC 1 cut(s) 419
Fnu4HI GCNGC 1 cut(s) 196
FokI GGATG 4 cut(s) 356, 374, 438, 514
Fsp4HI GCNGC 1 cut(s) 196
FspBI CTAG 5 cut(s) 312, 344, 456, 957, 965
GluI GCNGC 1 cut(s) 196
Hin1II CATG 9 cut(s) 20, 29, 467, 551, 662, 702, 877, 886, 910
HincII GTYRAC 1 cut(s) 94
HindII GTYRAC 1 cut(s) 94
HindIII AAGCTT 1 cut(s) 355
HinfI GANTC 7 cut(s) 143, 302, 366, 488, 634, 719, 815
HphI GGTGA 3 cut(s) 92, 571, 581
Hpy166II GTNNAC 2 cut(s) 94, 769
Hpy188I TCNGA 5 cut(s) 484, 718, 745, 945, 975
Hpy188III TCNNGA 7 cut(s) 26, 59, 110, 351, 393, 907, 989
Hpy8I GTNNAC 2 cut(s) 94, 769
Hpy99I CGWCG 1 cut(s) 176
HpyAV CCTTC 1 cut(s) 410
HpyCH4III ACNGT 2 cut(s) 187, 339
HpyCH4IV ACGT 1 cut(s) 983
HpyCH4V TGCA 8 cut(s) 89, 116, 195, 221, 494, 600, 839, 917
HpyF10VI GCNNNNNNNGC 1 cut(s) 852
HpyF3I CTNAG 1 cut(s) 316
HpySE526I ACGT 1 cut(s) 983
Hsp92II CATG 9 cut(s) 20, 29, 467, 551, 662, 702, 877, 886, 910
Kzo9I GATC 4 cut(s) 103, 205, 441, 885
LguI GCTCTTC 1 cut(s) 27
Lsp1109I GCAGC 1 cut(s) 207
LweI GCATC 4 cut(s) 103, 356, 398, 460
MaeI CTAG 5 cut(s) 312, 344, 456, 957, 965
MaeII ACGT 1 cut(s) 983
MaeIII GTNAC 3 cut(s) 569, 671, 733
MalI GATC 4 cut(s) 105, 207, 443, 887
MboI GATC 4 cut(s) 103, 205, 441, 885
MflI RGATCY 2 cut(s) 103, 205
MluCI AATT 5 cut(s) 84, 375, 432, 532, 1062
MlyI GAGTC 1 cut(s) 482
MnlI CCTC 5 cut(s) 43, 236, 452, 870, 943
MroXI GAANNNNTTC 1 cut(s) 105
MseI TTAA 2 cut(s) 531, 1075
MslI CAYNNNNRTG 1 cut(s) 659
MspR9I CCNGG 2 cut(s) 423, 800
Mva1269I GAATGC 3 cut(s) 514, 654, 846
MvaI CCWGG 2 cut(s) 423, 800
MwoI GCNNNNNNNGC 1 cut(s) 852
NdeII GATC 4 cut(s) 103, 205, 441, 885
NheI GCTAGC 1 cut(s) 343
NlaIII CATG 9 cut(s) 20, 29, 467, 551, 662, 702, 877, 886, 910
NlaIV GGNNCC 1 cut(s) 523
NmuCI GTSAC 2 cut(s) 569, 671
NspI RCATGY 1 cut(s) 702
PagI TCATGA 2 cut(s) 25, 906
PciI ACATGT 1 cut(s) 698
PciSI GCTCTTC 1 cut(s) 27
PctI GAATGC 3 cut(s) 514, 654, 846
PdmI GAANNNNTTC 1 cut(s) 105
PfeI GAWTC 6 cut(s) 143, 302, 366, 634, 719, 815
PflMI CCANNNNNTGG 1 cut(s) 644
PkrI GCNGC 1 cut(s) 197
PleI GAGTC 1 cut(s) 482
PpsI GAGTC 1 cut(s) 482
PpuMI RGGWCCY 1 cut(s) 419
PscI ACATGT 1 cut(s) 698
Psp5II RGGWCCY 1 cut(s) 419
Psp6I CCWGG 2 cut(s) 421, 798
PspGI CCWGG 2 cut(s) 421, 798
PspN4I GGNNCC 1 cut(s) 523
PspPI GGNCC 1 cut(s) 419
PspPPI RGGWCCY 1 cut(s) 419
PstI CTGCAG 1 cut(s) 496
PsuI RGATCY 2 cut(s) 103, 205
RsaI GTAC 3 cut(s) 328, 666, 864
RsaNI GTAC 3 cut(s) 327, 665, 863
RseI CAYNNNNRTG 1 cut(s) 659
SapI GCTCTTC 1 cut(s) 27
SaqAI TTAA 2 cut(s) 531, 1075
SatI GCNGC 1 cut(s) 196
Sau3AI GATC 4 cut(s) 103, 205, 441, 885
Sau96I GGNCC 1 cut(s) 419
ScaI AGTACT 1 cut(s) 328
SchI GAGTC 1 cut(s) 482
ScrFI CCNGG 2 cut(s) 423, 800
SfaNI GCATC 4 cut(s) 103, 356, 398, 460
SfcI CTRYAG 3 cut(s) 492, 687, 931
SinI GGWCC 1 cut(s) 419
SmiMI CAYNNNNRTG 1 cut(s) 659
Sse9I AATT 5 cut(s) 84, 375, 432, 532, 1062
SsiI CCGC 1 cut(s) 426
SspMI CTAG 5 cut(s) 312, 344, 456, 957, 965
StyD4I CCNGG 2 cut(s) 421, 798
StyI CCWWGG 1 cut(s) 43
TaaI ACNGT 2 cut(s) 187, 339
TaiI ACGT 1 cut(s) 986
TaqI TCGA 2 cut(s) 60, 888
TasI AATT 5 cut(s) 84, 375, 432, 532, 1062
TatI WGTACW 1 cut(s) 326
TfiI GAWTC 6 cut(s) 143, 302, 366, 634, 719, 815
Tru1I TTAA 2 cut(s) 531, 1075
Tru9I TTAA 2 cut(s) 531, 1075
TscAI CASTG 1 cut(s) 607
TseFI GTSAC 2 cut(s) 569, 671
TseI GCWGC 1 cut(s) 195
Tsp45I GTSAC 2 cut(s) 569, 671
TspDTI ATGAA 8 cut(s) 14, 42, 78, 138, 647, 839, 923, 1055
TspGWI ACGGA 1 cut(s) 282
TspRI CASTG 1 cut(s) 607
Van91I CCANNNNNTGG 1 cut(s) 644
VpaK11BI GGWCC 1 cut(s) 419
XapI RAATTY 1 cut(s) 375
XceI RCATGY 1 cut(s) 702
XmnI GAANNNNTTC 1 cut(s) 105
XspI CTAG 5 cut(s) 312, 344, 456, 957, 965
ZrmI AGTACT 1 cut(s) 328
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.