pycom02g25340

ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0001508
Physical Location & Seq
Forward (+)
23321849 .. 23324170
2322 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g25340.3

Sequence Viewer

Length: 858 bp
ATGAAAGTACGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGGTCGGGTTTCGTATTGGGGCTGGTCATCGAGTCCATATCAAACCCAAGCAACCACCTCAACACCACCGACTTTTCCTGCTCGAAGAACCTCTTCAATATCAACCCCGGCACCCGCCCGGCCGATATCACCGCCGCCAAGTCCTTCGGCAGACTCTCCGACGCCTCGGCCATGGCGGTGGATGCAGATTTTGAAACCCCGTCTCCTCCGTTTTACTCCCCTTCTTCCCATTTCAGCCCGCAGCGCCACTTCTACCTCGCCGTCGACAGACACCAGTTCAAGATGGAGACGTTGATGGACTTATTGGGCGTGGCGGGTCGGCGTCCTGGGTTGCCCATGGTGGTCTGCTGCAGCTCACGTGACGAGCTCGACGCCGTTAGCTCCGCCGTCGGCAACGTCCCCTATATCTCTTTGGCTTCTCTGTACACTGACCTTGCTGAAGCAGACCGTTCTTTAATATTAGAGCATTTTCGGCAAGCAACAATGAGGTGGAACCCAGAATTGAGTGCTCAATCAGCAGTTGATAATGAGTCTGTGAAAGATGAACAAAAATCTCATATGATAGTTGCAACAGATGCATGCCTTCCGCTGCTTGCTTCTGGGGAGTCACCTATAGCTGCCCGTGTTCTTATAAATTATGAGTTACCGACAAAGAAGGAAACATATATAAGGCGTATGACAACTTGTTTGGCTGCAGATGGGATTGTAATCAACATGGTCGTCGGGGGTGAGGTAGTAACTCTCAAAAGTCTTGAAGAAAGTAGCAACCTGGTCATTGCTGAGATGCCCATACATATTTCTGAGATCTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko03015 ko03040 map03013 map03015 map03040
Pfam Domains
Protein Families
Helicase_C

Protein Analysis

286

Amino Acids

31.5

Weight (kDa)

5.34

Isoelectric Point (pI)

52.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 677
AccB1I GGYRCC 1 cut(s) 155
AccI GTMKAC 1 cut(s) 309
AciI CCGC 8 cut(s) 160, 177, 180, 221, 284, 359, 429, 632
AcoI YGGCCR 2 cut(s) 165, 213
AcuI CTGAAG 1 cut(s) 504
AcvI CACGTG 1 cut(s) 404
AcyI GRCGYC 3 cut(s) 207, 367, 417
AfaI GTAC 2 cut(s) 9, 470
AgsI TTSAA 4 cut(s) 142, 239, 325, 800
AjnI CCWGG 2 cut(s) 370, 813
AluBI AGCT 4 cut(s) 399, 412, 426, 662
AluI AGCT 4 cut(s) 399, 412, 426, 662
Alw21I GWGCWC 2 cut(s) 414, 556
Alw26I GTCTC 2 cut(s) 252, 326
AoxI GGCC 2 cut(s) 165, 213
ApeKI GCWGC 6 cut(s) 286, 393, 396, 634, 662, 737
ArsI GACNNNNNNTTYG 2 cut(s) 715, 747
Asp700I GAANNNNTTC 1 cut(s) 137
AspLEI GCGC 1 cut(s) 291
AsuC2I CCSGG 2 cut(s) 153, 164
AsuHPI GGTGA 3 cut(s) 166, 645, 785
BanI GGYRCC 1 cut(s) 155
BanII GRGCYC 1 cut(s) 414
BbrPI CACGTG 1 cut(s) 404
Bbv12I GWGCWC 2 cut(s) 414, 556
BbvI GCAGC 6 cut(s) 298, 380, 408, 621, 649, 724
BccI CCATC 3 cut(s) 322, 334, 737
BceAI ACGGC 3 cut(s) 290, 404, 416
BciT130I CCWGG 2 cut(s) 372, 815
BcnI CCSGG 2 cut(s) 153, 164
BcoDI GTCTC 2 cut(s) 252, 326
BfmI CTRYAG 3 cut(s) 394, 657, 738
BfoI RGCGCY 1 cut(s) 292
BglII AGATCT 1 cut(s) 849
BisI GCNGC 7 cut(s) 180, 287, 394, 397, 635, 663, 738
BlsI GCNGC 7 cut(s) 181, 288, 395, 398, 636, 664, 739
Bme1390I CCNGG 4 cut(s) 153, 164, 372, 815
BmiI GGNNCC 2 cut(s) 157, 539
BmrFI CCNGG 4 cut(s) 153, 164, 372, 815
BmsI GCATC 3 cut(s) 217, 610, 819
BpuMI CCSGG 2 cut(s) 153, 164
BsaAI YACGTR 1 cut(s) 404
BsaBI GATNNNNATC 1 cut(s) 752
BsaHI GRCGYC 3 cut(s) 207, 367, 417
BsaJI CCNNGG 5 cut(s) 151, 210, 216, 371, 381
BsaXI ACNNNNNCTCC 2 cut(s) 232, 262
Bse1I ACTGG 1 cut(s) 319
Bse3DI GCAATG 1 cut(s) 819
Bse8I GATNNNNATC 1 cut(s) 752
BseBI CCWGG 2 cut(s) 372, 815
BseDI CCNNGG 5 cut(s) 151, 210, 216, 371, 381
BseGI GGATG 1 cut(s) 232
BseJI GATNNNNATC 1 cut(s) 752
BseMI GCAATG 1 cut(s) 819
BseMII CTCAG 2 cut(s) 816, 837
BseNI ACTGG 1 cut(s) 319
BseRI GAGGAG 1 cut(s) 240
BseX3I CGGCCG 1 cut(s) 165
BseXI GCAGC 6 cut(s) 298, 380, 408, 621, 649, 724
Bsh1285I CGRYCG 1 cut(s) 168
BshFI GGCC 2 cut(s) 167, 215
BshNI GGYRCC 1 cut(s) 155
BsiEI CGRYCG 1 cut(s) 168
BsiHKAI GWGCWC 2 cut(s) 414, 556
BsiSI CCGG 2 cut(s) 153, 164
BslFI GGGAC 1 cut(s) 428
BsmAI GTCTC 2 cut(s) 252, 326
BsmBI CGTCTC 2 cut(s) 252, 326
BsmFI GGGAC 1 cut(s) 428
BsnI GGCC 2 cut(s) 167, 215
Bsp1286I GDGCHC 2 cut(s) 414, 556
Bsp1407I TGTACA 1 cut(s) 468
Bsp143I GATC 1 cut(s) 849
Bsp19I CCATGG 2 cut(s) 216, 381
BspACI CCGC 8 cut(s) 160, 177, 180, 221, 284, 359, 429, 632
BspANI GGCC 2 cut(s) 167, 215
BspCNI CTCAG 2 cut(s) 817, 838
BspLI GGNNCC 2 cut(s) 157, 539
BspMAI CTGCAG 2 cut(s) 398, 742
BspT107I GGYRCC 1 cut(s) 155
BsrDI GCAATG 1 cut(s) 819
BsrGI TGTACA 1 cut(s) 468
BsrI ACTGG 1 cut(s) 319
BssECI CCNNGG 5 cut(s) 151, 210, 216, 371, 381
BssMI GATC 1 cut(s) 849
BssNI GRCGYC 3 cut(s) 207, 367, 417
BssT1I CCWWGG 2 cut(s) 216, 381
Bst2UI CCWGG 2 cut(s) 372, 815
Bst4CI ACNGT 1 cut(s) 494
Bst6I CTCTTC 1 cut(s) 143
BstACI GRCGYC 3 cut(s) 207, 367, 417
BstAPI GCANNNNNTGC 1 cut(s) 620
BstAUI TGTACA 1 cut(s) 468
BstBAI YACGTR 1 cut(s) 404
BstC8I GCNNGC 4 cut(s) 284, 522, 625, 639
BstDEI CTNAG 2 cut(s) 825, 846
BstDSI CCRYGG 2 cut(s) 216, 381
BstF5I GGATG 1 cut(s) 232
BstH2I RGCGCY 1 cut(s) 292
BstHHI GCGC 1 cut(s) 291
BstKTI GATC 1 cut(s) 852
BstMAI GTCTC 2 cut(s) 252, 326
BstMBI GATC 1 cut(s) 849
BstMCI CGRYCG 1 cut(s) 168
BstMWI GCNNNNNNNGC 5 cut(s) 227, 288, 517, 560, 620
BstNI CCWGG 2 cut(s) 372, 815
BstNSI RCATGY 1 cut(s) 627
BstSCI CCNGG 4 cut(s) 151, 162, 370, 813
BstSFI CTRYAG 3 cut(s) 394, 657, 738
BstV1I GCAGC 6 cut(s) 298, 380, 408, 621, 649, 724
BstX2I RGATCY 1 cut(s) 849
BstXI CCANNNNNNTGG 1 cut(s) 223
BstYI RGATCY 1 cut(s) 849
BstZI CGGCCG 1 cut(s) 165
BsuRI GGCC 2 cut(s) 167, 215
BtgI CCRYGG 2 cut(s) 216, 381
BtsCI GGATG 1 cut(s) 232
BtsIMutI CAGTG 1 cut(s) 471
Cac8I GCNNGC 4 cut(s) 284, 522, 625, 639
CfoI GCGC 1 cut(s) 291
CseI GACGC 3 cut(s) 215, 356, 425
CsiI ACCWGGT 1 cut(s) 813
Csp6I GTAC 2 cut(s) 8, 469
CviAII CATG 4 cut(s) 217, 382, 624, 760
CviQI GTAC 2 cut(s) 8, 469
DdeI CTNAG 2 cut(s) 825, 846
DpnI GATC 1 cut(s) 851
DpnII GATC 1 cut(s) 849
EaeI YGGCCR 2 cut(s) 165, 213
EagI CGGCCG 1 cut(s) 165
Eam1104I CTCTTC 1 cut(s) 143
EarI CTCTTC 1 cut(s) 143
EciI GGCGGA 1 cut(s) 418
Ecl136II GAGCTC 1 cut(s) 412
EclXI CGGCCG 1 cut(s) 165
Eco130I CCWWGG 2 cut(s) 216, 381
Eco24I GRGCYC 1 cut(s) 414
Eco32I GATATC 1 cut(s) 172
Eco52I CGGCCG 1 cut(s) 165
Eco53kI GAGCTC 1 cut(s) 412
Eco57I CTGAAG 1 cut(s) 504
Eco72I CACGTG 1 cut(s) 404
EcoICRI GAGCTC 1 cut(s) 412
EcoRII CCWGG 2 cut(s) 370, 813
EcoRV GATATC 1 cut(s) 172
EcoT14I CCWWGG 2 cut(s) 216, 381
EcoT22I ATGCAT 1 cut(s) 625
EcoT38I GRGCYC 1 cut(s) 414
ErhI CCWWGG 2 cut(s) 216, 381
Esp3I CGTCTC 2 cut(s) 252, 326
FaeI CATG 4 cut(s) 220, 385, 627, 763
FalI AAGNNNNNCTT 2 cut(s) 122, 154
FaqI GGGAC 1 cut(s) 428
FatI CATG 4 cut(s) 216, 381, 623, 759
FauI CCCGC 3 cut(s) 167, 291, 352
FauNDI CATATG 1 cut(s) 603
FblI GTMKAC 1 cut(s) 309
Fnu4HI GCNGC 7 cut(s) 180, 287, 394, 397, 635, 663, 738
FokI GGATG 1 cut(s) 239
FriOI GRGCYC 1 cut(s) 414
Fsp4HI GCNGC 7 cut(s) 180, 287, 394, 397, 635, 663, 738
GlaI GCGC 1 cut(s) 290
GluI GCNGC 7 cut(s) 180, 287, 394, 397, 635, 663, 738
HaeII RGCGCY 1 cut(s) 292
HaeIII GGCC 2 cut(s) 167, 215
HapII CCGG 2 cut(s) 153, 164
HgaI GACGC 3 cut(s) 215, 356, 425
HhaI GCGC 1 cut(s) 291
Hin1I GRCGYC 3 cut(s) 207, 367, 417
Hin1II CATG 4 cut(s) 220, 385, 627, 763
Hin6I GCGC 1 cut(s) 289
HinP1I GCGC 1 cut(s) 289
HincII GTYRAC 1 cut(s) 310
HindII GTYRAC 1 cut(s) 310
HinfI GANTC 4 cut(s) 77, 198, 575, 650
HpaII CCGG 2 cut(s) 153, 164
HphI GGTGA 3 cut(s) 166, 645, 785
Hpy166II GTNNAC 2 cut(s) 310, 471
Hpy188I TCNGA 2 cut(s) 205, 847
Hpy188III TCNNGA 2 cut(s) 325, 797
Hpy8I GTNNAC 2 cut(s) 310, 471
Hpy99I CGWCG 5 cut(s) 209, 311, 419, 437, 770
HpyAV CCTTC 4 cut(s) 199, 276, 638, 694
HpyCH4III ACNGT 1 cut(s) 494
HpyCH4IV ACGT 3 cut(s) 335, 403, 441
HpyCH4V TGCA 5 cut(s) 230, 396, 614, 623, 740
HpyF10VI GCNNNNNNNGC 5 cut(s) 227, 288, 517, 560, 620
HpyF3I CTNAG 2 cut(s) 825, 846
HpySE526I ACGT 3 cut(s) 335, 403, 441
Hsp92I GRCGYC 3 cut(s) 207, 367, 417
Hsp92II CATG 4 cut(s) 220, 385, 627, 763
HspAI GCGC 1 cut(s) 289
Kzo9I GATC 1 cut(s) 849
LmnI GCTCC 1 cut(s) 431
Lsp1109I GCAGC 6 cut(s) 298, 380, 408, 621, 649, 724
LweI GCATC 3 cut(s) 217, 610, 819
MabI ACCWGGT 1 cut(s) 813
MaeII ACGT 3 cut(s) 335, 403, 441
MaeIII GTNAC 4 cut(s) 404, 651, 687, 781
MalI GATC 1 cut(s) 851
MboI GATC 1 cut(s) 849
MboII GAAGA 4 cut(s) 130, 142, 261, 812
MflI RGATCY 1 cut(s) 849
MhlI GDGCHC 2 cut(s) 414, 556
MluCI AATT 2 cut(s) 545, 679
MlyI GAGTC 4 cut(s) 86, 192, 584, 659
MmeI TCCRAC 1 cut(s) 228
MnlI CCTC 8 cut(s) 39, 113, 146, 220, 261, 311, 525, 769
Mph1103I ATGCAT 1 cut(s) 625
MroXI GAANNNNTTC 1 cut(s) 137
MseI TTAA 1 cut(s) 500
MslI CAYNNNNRTG 1 cut(s) 221
MspA1I CMGCKG 1 cut(s) 634
MspI CCGG 2 cut(s) 153, 164
MspR9I CCNGG 4 cut(s) 153, 164, 372, 815
MvaI CCWGG 2 cut(s) 372, 815
MwoI GCNNNNNNNGC 5 cut(s) 227, 288, 517, 560, 620
NciI CCSGG 2 cut(s) 153, 164
NcoI CCATGG 2 cut(s) 216, 381
NdeI CATATG 1 cut(s) 603
NdeII GATC 1 cut(s) 849
NlaIII CATG 4 cut(s) 220, 385, 627, 763
NlaIV GGNNCC 2 cut(s) 157, 539
NmeAIII GCCGAG 1 cut(s) 191
NmuCI GTSAC 2 cut(s) 404, 651
NsiI ATGCAT 1 cut(s) 625
NspI RCATGY 1 cut(s) 627
PaeI GCATGC 1 cut(s) 627
PdmI GAANNNNTTC 1 cut(s) 137
PkrI GCNGC 7 cut(s) 181, 288, 395, 398, 636, 664, 739
PleI GAGTC 4 cut(s) 85, 192, 583, 658
PmaCI CACGTG 1 cut(s) 404
PmlI CACGTG 1 cut(s) 404
PpsI GAGTC 4 cut(s) 85, 192, 583, 658
Ppu21I YACGTR 1 cut(s) 404
PsiI TTATAA 1 cut(s) 677
Psp124BI GAGCTC 1 cut(s) 414
Psp6I CCWGG 2 cut(s) 370, 813
PspCI CACGTG 1 cut(s) 404
PspGI CCWGG 2 cut(s) 370, 813
PspN4I GGNNCC 2 cut(s) 157, 539
PstI CTGCAG 2 cut(s) 398, 742
PsuI RGATCY 1 cut(s) 849
RsaI GTAC 2 cut(s) 9, 470
RsaNI GTAC 2 cut(s) 8, 469
RseI CAYNNNNRTG 1 cut(s) 221
SacI GAGCTC 1 cut(s) 414
SalI GTCGAC 1 cut(s) 308
SaqAI TTAA 1 cut(s) 500
SatI GCNGC 7 cut(s) 180, 287, 394, 397, 635, 663, 738
Sau3AI GATC 1 cut(s) 849
SchI GAGTC 4 cut(s) 86, 192, 584, 659
ScrFI CCNGG 4 cut(s) 153, 164, 372, 815
SduI GDGCHC 2 cut(s) 414, 556
SexAI ACCWGGT 1 cut(s) 813
SfaNI GCATC 3 cut(s) 217, 610, 819
SfcI CTRYAG 3 cut(s) 394, 657, 738
SmiMI CAYNNNNRTG 1 cut(s) 221
SphI GCATGC 1 cut(s) 627
Sse9I AATT 2 cut(s) 545, 679
SsiI CCGC 8 cut(s) 160, 177, 180, 221, 284, 359, 429, 632
SspI AATATT 1 cut(s) 504
SstI GAGCTC 1 cut(s) 414
StyD4I CCNGG 4 cut(s) 151, 162, 370, 813
StyI CCWWGG 2 cut(s) 216, 381
TaaI ACNGT 1 cut(s) 494
TaiI ACGT 3 cut(s) 338, 406, 444
TaqI TCGA 4 cut(s) 75, 128, 309, 414
TasI AATT 2 cut(s) 545, 679
TatI WGTACW 1 cut(s) 468
TauI GCSGC 1 cut(s) 182
Tru1I TTAA 1 cut(s) 500
Tru9I TTAA 1 cut(s) 500
TscAI CASTG 1 cut(s) 478
TseFI GTSAC 2 cut(s) 404, 651
TseI GCWGC 6 cut(s) 286, 393, 396, 634, 662, 737
Tsp45I GTSAC 2 cut(s) 404, 651
TspDTI ATGAA 2 cut(s) 17, 603
TspGWI ACGGA 1 cut(s) 243
TspRI CASTG 1 cut(s) 478
XceI RCATGY 1 cut(s) 627
XmiI GTMKAC 1 cut(s) 309
XmnI GAANNNNTTC 1 cut(s) 137
Zsp2I ATGCAT 1 cut(s) 625
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.