Rroxscaffold_2G00138270

ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0001508
Physical Location & Seq
Forward (+)
76392759 .. 76396052
3294 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00138270.1

Sequence Viewer

Length: 834 bp
ATGAGACGAGAAAACACACCTTCTAAAATGGAATGGACAAGCCCAAAGCCACCTCTGGGCCTGGGCCTCTCTGATAAGATGTGGCATGTTCTGAAAACGGAAGGGACATTCGAAGCCGAAGGTGAGCTCGGTTCAGCTCCCTCTCCTCCATCTCTATCACCTTCCCATTTCAGTCAACAGCGCCACTTCTATCTCGCCGTCGACAGACTTCAGTTCAAGTTGGAGACATTTATGGACCTCTTGGGCGTGGCGGGAAGGCGCCCCTGTCTTCCAATGGTGGTCTGCTGCAGCTCACGTGACGAACTCGACGCCGTCAGCTCCGCCGTCGCAACCGTCCCCTATATCTCTCTGGCCCCTCTGTTAGATGTAGAGGAGGAAGAAACAGATCATAGTTTGTGCTATAGCTGTTGCCGTTCTAACCTTGAAATTGTTGTTGTCTATGCGTACACTGACCTAGCTGAAGCAGACCGTGCTGCGATATTAGAGTCCTTCAAGCAAGCAACCATCAGGTGGAACCCAGAAGTTGGCGCTCAATCAGCTGATGACAGTGAGCCTGTGAAAGAGGAACAAAAATCTCATATGATAGTTGCTACAGATGCATGCATTCCGCTTCTTGCTTCTGGGGAGTCACCTATTTCTGCTCGTGTCCTTATAAACTATGAGTTACCTGCAAAGAAGGAAATATATACGAGGCGTATGGCAACTTGTTTGGCTGCAGATGGGATTGTAATCAATATGGTTGTGGGGGGTGAGGTAGTAACTCTCAAAAGTATTGAAGATAGCAGCAACCTAGTCATAGCTGAGATGCCCATAAATATTTCTGAGATTTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko03015 ko03040 map03013 map03015 map03040
Pfam Domains
Protein Families
Helicase_C

Protein Analysis

277

Amino Acids

30.39

Weight (kDa)

4.74

Isoelectric Point (pI)

63.67

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 653
Acc36I ACCTGC 1 cut(s) 676
AccB1I GGYRCC 1 cut(s) 258
AccB7I CCANNNNNTGG 2 cut(s) 510, 524
AccI GTMKAC 1 cut(s) 201
AciI CCGC 3 cut(s) 251, 321, 608
AcuI CTGAAG 2 cut(s) 194, 480
AcvI CACGTG 1 cut(s) 296
AcyI GRCGYC 2 cut(s) 259, 309
AfaI GTAC 1 cut(s) 446
AfiI CCNNNNNNNGG 3 cut(s) 56, 510, 524
AgsI TTSAA 4 cut(s) 217, 425, 493, 776
AjnI CCWGG 1 cut(s) 60
AluBI AGCT 8 cut(s) 127, 137, 291, 318, 405, 458, 539, 800
AluI AGCT 8 cut(s) 127, 137, 291, 318, 405, 458, 539, 800
Alw21I GWGCWC 1 cut(s) 129
Alw26I GTCTC 1 cut(s) 218
AoxI GGCC 3 cut(s) 58, 64, 351
ApeKI GCWGC 5 cut(s) 285, 288, 473, 713, 783
AspLEI GCGC 3 cut(s) 183, 261, 530
AspS9I GGNCC 4 cut(s) 58, 64, 235, 352
AsuHPI GGTGA 4 cut(s) 134, 150, 621, 761
AsuII TTCGAA 1 cut(s) 111
AvaII GGWCC 1 cut(s) 235
BanI GGYRCC 1 cut(s) 258
BanII GRGCYC 1 cut(s) 129
BauI CACGAG 1 cut(s) 642
BbrPI CACGTG 1 cut(s) 296
BbsI GAAGAC 1 cut(s) 260
Bbv12I GWGCWC 1 cut(s) 129
BbvI GCAGC 5 cut(s) 272, 300, 460, 700, 795
BccI CCATC 3 cut(s) 157, 512, 713
BceAI ACGGC 4 cut(s) 182, 296, 308, 396
BciT130I CCWGG 1 cut(s) 62
BcoDI GTCTC 1 cut(s) 218
BfaI CTAG 2 cut(s) 455, 791
BfmI CTRYAG 4 cut(s) 286, 400, 591, 714
BfoI RGCGCY 3 cut(s) 184, 262, 531
BfuAI ACCTGC 1 cut(s) 676
BisI GCNGC 5 cut(s) 286, 289, 474, 714, 784
BlsI GCNGC 5 cut(s) 287, 290, 475, 715, 785
Bme1390I CCNGG 1 cut(s) 62
Bme18I GGWCC 1 cut(s) 235
BmgT120I GGNCC 4 cut(s) 58, 64, 235, 352
BmiI GGNNCC 3 cut(s) 260, 354, 515
BmrFI CCNGG 1 cut(s) 62
BmsI GCATC 2 cut(s) 586, 795
BpiI GAAGAC 1 cut(s) 260
Bpu14I TTCGAA 1 cut(s) 111
BsaAI YACGTR 1 cut(s) 296
BsaBI GATNNNNATC 1 cut(s) 728
BsaHI GRCGYC 2 cut(s) 259, 309
BsaJI CCNNGG 1 cut(s) 61
Bsc4I CCNNNNNNNGG 3 cut(s) 56, 510, 524
Bse8I GATNNNNATC 1 cut(s) 728
BseBI CCWGG 1 cut(s) 62
BseDI CCNNGG 1 cut(s) 61
BseJI GATNNNNATC 1 cut(s) 728
BseLI CCNNNNNNNGG 3 cut(s) 56, 510, 524
BseMII CTCAG 2 cut(s) 792, 813
BseRI GAGGAG 2 cut(s) 135, 386
BseXI GCAGC 5 cut(s) 272, 300, 460, 700, 795
BshFI GGCC 3 cut(s) 60, 66, 353
BshNI GGYRCC 1 cut(s) 258
BsiHKAI GWGCWC 1 cut(s) 129
BslFI GGGAC 2 cut(s) 118, 320
BslI CCNNNNNNNGG 3 cut(s) 56, 510, 524
BsmAI GTCTC 1 cut(s) 218
BsmFI GGGAC 2 cut(s) 118, 320
BsmI GAATGC 1 cut(s) 603
BsnI GGCC 3 cut(s) 60, 66, 353
Bsp119I TTCGAA 1 cut(s) 111
Bsp1286I GDGCHC 1 cut(s) 129
Bsp143I GATC 1 cut(s) 385
BspACI CCGC 3 cut(s) 251, 321, 608
BspANI GGCC 3 cut(s) 60, 66, 353
BspCNI CTCAG 2 cut(s) 793, 814
BspLI GGNNCC 3 cut(s) 260, 354, 515
BspMAI CTGCAG 2 cut(s) 290, 718
BspMI ACCTGC 1 cut(s) 676
BspT104I TTCGAA 1 cut(s) 111
BspT107I GGYRCC 1 cut(s) 258
BssECI CCNNGG 1 cut(s) 61
BssMI GATC 1 cut(s) 385
BssNI GRCGYC 2 cut(s) 259, 309
BssSI CACGAG 1 cut(s) 642
Bst2BI CACGAG 1 cut(s) 642
Bst2UI CCWGG 1 cut(s) 62
Bst4CI ACNGT 3 cut(s) 334, 470, 548
BstACI GRCGYC 2 cut(s) 259, 309
BstAPI GCANNNNNTGC 1 cut(s) 470
BstBAI YACGTR 1 cut(s) 296
BstBI TTCGAA 1 cut(s) 111
BstC8I GCNNGC 2 cut(s) 498, 601
BstDEI CTNAG 2 cut(s) 801, 822
BstH2I RGCGCY 3 cut(s) 184, 262, 531
BstHHI GCGC 3 cut(s) 183, 261, 530
BstKTI GATC 1 cut(s) 388
BstMAI GTCTC 1 cut(s) 218
BstMBI GATC 1 cut(s) 385
BstMWI GCNNNNNNNGC 3 cut(s) 470, 536, 596
BstNI CCWGG 1 cut(s) 62
BstNSI RCATGY 2 cut(s) 89, 603
BstSCI CCNGG 1 cut(s) 60
BstSFI CTRYAG 4 cut(s) 286, 400, 591, 714
BstV1I GCAGC 5 cut(s) 272, 300, 460, 700, 795
BstV2I GAAGAC 1 cut(s) 260
BsuRI GGCC 3 cut(s) 60, 66, 353
BtsIMutI CAGTG 2 cut(s) 447, 553
BveI ACCTGC 1 cut(s) 676
Cac8I GCNNGC 2 cut(s) 498, 601
CfoI GCGC 3 cut(s) 183, 261, 530
Cfr13I GGNCC 4 cut(s) 58, 64, 235, 352
CseI GACGC 1 cut(s) 317
Csp6I GTAC 1 cut(s) 445
CviAII CATG 2 cut(s) 86, 600
CviQI GTAC 1 cut(s) 445
DdeI CTNAG 2 cut(s) 801, 822
DinI GGCGCC 1 cut(s) 260
DpnI GATC 1 cut(s) 387
DpnII GATC 1 cut(s) 385
EciI GGCGGA 1 cut(s) 310
Ecl136II GAGCTC 1 cut(s) 127
Eco24I GRGCYC 1 cut(s) 129
Eco47I GGWCC 1 cut(s) 235
Eco53kI GAGCTC 1 cut(s) 127
Eco57I CTGAAG 2 cut(s) 194, 480
Eco72I CACGTG 1 cut(s) 296
EcoICRI GAGCTC 1 cut(s) 127
EcoRII CCWGG 1 cut(s) 60
EcoT22I ATGCAT 2 cut(s) 601, 605
EcoT38I GRGCYC 1 cut(s) 129
EgeI GGCGCC 1 cut(s) 260
EheI GGCGCC 1 cut(s) 260
FaeI CATG 2 cut(s) 89, 603
FaqI GGGAC 2 cut(s) 118, 320
FatI CATG 2 cut(s) 85, 599
FauI CCCGC 1 cut(s) 244
FauNDI CATATG 1 cut(s) 579
FblI GTMKAC 1 cut(s) 201
Fnu4HI GCNGC 5 cut(s) 286, 289, 474, 714, 784
FriOI GRGCYC 1 cut(s) 129
Fsp4HI GCNGC 5 cut(s) 286, 289, 474, 714, 784
FspBI CTAG 2 cut(s) 455, 791
GlaI GCGC 3 cut(s) 182, 260, 529
GluI GCNGC 5 cut(s) 286, 289, 474, 714, 784
HaeII RGCGCY 3 cut(s) 184, 262, 531
HaeIII GGCC 3 cut(s) 60, 66, 353
HgaI GACGC 1 cut(s) 317
HhaI GCGC 3 cut(s) 183, 261, 530
Hin1I GRCGYC 2 cut(s) 259, 309
Hin1II CATG 2 cut(s) 89, 603
Hin6I GCGC 3 cut(s) 181, 259, 528
HinP1I GCGC 3 cut(s) 181, 259, 528
HincII GTYRAC 2 cut(s) 176, 202
HindII GTYRAC 2 cut(s) 176, 202
HinfI GANTC 2 cut(s) 485, 626
HphI GGTGA 4 cut(s) 134, 150, 621, 761
Hpy166II GTNNAC 3 cut(s) 176, 202, 447
Hpy188I TCNGA 3 cut(s) 73, 93, 823
Hpy8I GTNNAC 3 cut(s) 176, 202, 447
Hpy99I CGWCG 3 cut(s) 203, 311, 329
HpyAV CCTTC 7 cut(s) 30, 95, 113, 171, 249, 499, 670
HpyCH4III ACNGT 3 cut(s) 334, 470, 548
HpyCH4IV ACGT 1 cut(s) 295
HpyCH4V TGCA 5 cut(s) 288, 599, 603, 671, 716
HpyF10VI GCNNNNNNNGC 3 cut(s) 470, 536, 596
HpyF3I CTNAG 2 cut(s) 801, 822
HpySE526I ACGT 1 cut(s) 295
Hsp92I GRCGYC 2 cut(s) 259, 309
Hsp92II CATG 2 cut(s) 89, 603
HspAI GCGC 3 cut(s) 181, 259, 528
KasI GGCGCC 1 cut(s) 258
Kzo9I GATC 1 cut(s) 385
LmnI GCTCC 2 cut(s) 142, 323
Lsp1109I GCAGC 5 cut(s) 272, 300, 460, 700, 795
LweI GCATC 2 cut(s) 586, 795
MaeI CTAG 2 cut(s) 455, 791
MaeII ACGT 1 cut(s) 295
MaeIII GTNAC 4 cut(s) 296, 627, 663, 757
MalI GATC 1 cut(s) 387
MboI GATC 1 cut(s) 385
MboII GAAGA 3 cut(s) 260, 389, 788
MhlI GDGCHC 1 cut(s) 129
MluCI AATT 1 cut(s) 426
Mly113I GGCGCC 1 cut(s) 259
MlyI GAGTC 2 cut(s) 494, 635
MmeI TCCRAC 1 cut(s) 201
Mph1103I ATGCAT 2 cut(s) 601, 605
MspA1I CMGCKG 1 cut(s) 539
MspR9I CCNGG 1 cut(s) 62
Mva1269I GAATGC 1 cut(s) 603
MvaI CCWGG 1 cut(s) 62
MwoI GCNNNNNNNGC 3 cut(s) 470, 536, 596
NarI GGCGCC 1 cut(s) 259
NdeI CATATG 1 cut(s) 579
NdeII GATC 1 cut(s) 385
NlaIII CATG 2 cut(s) 89, 603
NlaIV GGNNCC 3 cut(s) 260, 354, 515
NmuCI GTSAC 2 cut(s) 296, 627
NsiI ATGCAT 2 cut(s) 601, 605
NspI RCATGY 2 cut(s) 89, 603
NspV TTCGAA 1 cut(s) 111
PaeI GCATGC 1 cut(s) 603
PctI GAATGC 1 cut(s) 603
PflFI GACNNNGTC 1 cut(s) 311
PflMI CCANNNNNTGG 2 cut(s) 510, 524
PkrI GCNGC 5 cut(s) 287, 290, 475, 715, 785
PleI GAGTC 2 cut(s) 493, 634
PluTI GGCGCC 1 cut(s) 262
PmaCI CACGTG 1 cut(s) 296
PmlI CACGTG 1 cut(s) 296
PpsI GAGTC 2 cut(s) 493, 634
Ppu21I YACGTR 1 cut(s) 296
PsiI TTATAA 1 cut(s) 653
Psp124BI GAGCTC 1 cut(s) 129
Psp6I CCWGG 1 cut(s) 60
PspCI CACGTG 1 cut(s) 296
PspGI CCWGG 1 cut(s) 60
PspN4I GGNNCC 3 cut(s) 260, 354, 515
PspPI GGNCC 4 cut(s) 58, 64, 235, 352
PstI CTGCAG 2 cut(s) 290, 718
PsyI GACNNNGTC 1 cut(s) 311
PvuII CAGCTG 1 cut(s) 539
RsaI GTAC 1 cut(s) 446
RsaNI GTAC 1 cut(s) 445
SacI GAGCTC 1 cut(s) 129
SalI GTCGAC 1 cut(s) 200
SatI GCNGC 5 cut(s) 286, 289, 474, 714, 784
Sau3AI GATC 1 cut(s) 385
Sau96I GGNCC 4 cut(s) 58, 64, 235, 352
SchI GAGTC 2 cut(s) 494, 635
ScrFI CCNGG 1 cut(s) 62
SduI GDGCHC 1 cut(s) 129
SfaNI GCATC 2 cut(s) 586, 795
SfcI CTRYAG 4 cut(s) 286, 400, 591, 714
SfoI GGCGCC 1 cut(s) 260
SfuI TTCGAA 1 cut(s) 111
SinI GGWCC 1 cut(s) 235
SphI GCATGC 1 cut(s) 603
Sse9I AATT 1 cut(s) 426
SsiI CCGC 3 cut(s) 251, 321, 608
SspDI GGCGCC 1 cut(s) 258
SspI AATATT 1 cut(s) 817
SspMI CTAG 2 cut(s) 455, 791
SstI GAGCTC 1 cut(s) 129
StyD4I CCNGG 1 cut(s) 60
TaaI ACNGT 3 cut(s) 334, 470, 548
TaiI ACGT 1 cut(s) 298
TaqI TCGA 3 cut(s) 111, 201, 306
TasI AATT 1 cut(s) 426
TscAI CASTG 2 cut(s) 454, 553
TseFI GTSAC 2 cut(s) 296, 627
TseI GCWGC 5 cut(s) 285, 288, 473, 713, 783
Tsp45I GTSAC 2 cut(s) 296, 627
TspGWI ACGGA 1 cut(s) 113
TspRI CASTG 2 cut(s) 454, 553
Tth111I GACNNNGTC 1 cut(s) 311
Van91I CCANNNNNTGG 2 cut(s) 510, 524
VpaK11BI GGWCC 1 cut(s) 235
XceI RCATGY 2 cut(s) 89, 603
XmiI GTMKAC 1 cut(s) 201
XspI CTAG 2 cut(s) 455, 791
Zsp2I ATGCAT 2 cut(s) 601, 605
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.