pycom02g25350

ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0001508
Physical Location & Seq
Reverse (-)
23330282 .. 23332124
1843 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g25350.2

Sequence Viewer

Length: 519 bp
ATGGACTTATTGGGCGTGGCGGGTCGGCGTCCTGGGTTGCCCATGGTGGTCTGCTGCAGCTCACGTGACGAGCTCGACGCCGTTAGCTCCGCCGTCGGCAACGTCCCCTATATCTCTTTGGCTTCTCTGTACACTGACCTTGCTGAAGCAGACCGTTCTTTAATATTAGAGCATTTTCGGCAAGCAACAATGAGGTGGAACCCAGAATTGAGTGCTCAATCAGCAGTTGATAATGAGTCTGTGAAAGATGAACAAAAATCTCATATGATAGTTGCAACAGATGCATGCCTTCCGCTGCTTGCTTCTGGGGAGTCACCTATAGCTGCCCGTGTTCTTATAAATTATGAGTTACCGACAAAGAAGGAAACATATATAAGGCGTATGACAACTTGTTTGGCTGCAGATGGGATTGTAATCAACATGGTCGTCGGGGGTGAGATAGTAACTCTCAAAAGTCTTGAAGAAAGTAGCAACCTGGTCATAGCTGAGATGCCCATACATATTTCTGAGATCTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko03015 ko03040 map03013 map03015 map03040
Pfam Domains
Protein Families
Helicase_C

Protein Analysis

173

Amino Acids

18.66

Weight (kDa)

4.76

Isoelectric Point (pI)

52.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 338
AciI CCGC 3 cut(s) 20, 90, 293
AcuI CTGAAG 1 cut(s) 165
AcvI CACGTG 1 cut(s) 65
AcyI GRCGYC 2 cut(s) 28, 78
AfaI GTAC 1 cut(s) 131
AgsI TTSAA 1 cut(s) 461
AjnI CCWGG 2 cut(s) 31, 474
AluBI AGCT 5 cut(s) 60, 73, 87, 323, 485
AluI AGCT 5 cut(s) 60, 73, 87, 323, 485
Alw21I GWGCWC 2 cut(s) 75, 217
ApeKI GCWGC 5 cut(s) 54, 57, 295, 323, 398
ArsI GACNNNNNNTTYG 2 cut(s) 376, 408
AsuHPI GGTGA 2 cut(s) 306, 446
BanII GRGCYC 1 cut(s) 75
BbrPI CACGTG 1 cut(s) 65
Bbv12I GWGCWC 2 cut(s) 75, 217
BbvI GCAGC 5 cut(s) 41, 69, 282, 310, 385
BccI CCATC 1 cut(s) 398
BceAI ACGGC 2 cut(s) 65, 77
BciT130I CCWGG 2 cut(s) 33, 476
BfmI CTRYAG 3 cut(s) 55, 318, 399
BglII AGATCT 1 cut(s) 510
BisI GCNGC 5 cut(s) 55, 58, 296, 324, 399
BlsI GCNGC 5 cut(s) 56, 59, 297, 325, 400
Bme1390I CCNGG 2 cut(s) 33, 476
BmiI GGNNCC 1 cut(s) 200
BmrFI CCNGG 2 cut(s) 33, 476
BmsI GCATC 2 cut(s) 271, 480
BsaAI YACGTR 1 cut(s) 65
BsaBI GATNNNNATC 1 cut(s) 413
BsaHI GRCGYC 2 cut(s) 28, 78
BsaJI CCNNGG 2 cut(s) 32, 42
Bse8I GATNNNNATC 1 cut(s) 413
BseBI CCWGG 2 cut(s) 33, 476
BseDI CCNNGG 2 cut(s) 32, 42
BseJI GATNNNNATC 1 cut(s) 413
BseMII CTCAG 2 cut(s) 477, 498
BseXI GCAGC 5 cut(s) 41, 69, 282, 310, 385
BsiHKAI GWGCWC 2 cut(s) 75, 217
BslFI GGGAC 1 cut(s) 89
BsmFI GGGAC 1 cut(s) 89
Bsp1286I GDGCHC 2 cut(s) 75, 217
Bsp1407I TGTACA 1 cut(s) 129
Bsp143I GATC 1 cut(s) 510
Bsp19I CCATGG 1 cut(s) 42
BspACI CCGC 3 cut(s) 20, 90, 293
BspCNI CTCAG 2 cut(s) 478, 499
BspLI GGNNCC 1 cut(s) 200
BspMAI CTGCAG 2 cut(s) 59, 403
BsrGI TGTACA 1 cut(s) 129
BssECI CCNNGG 2 cut(s) 32, 42
BssMI GATC 1 cut(s) 510
BssNI GRCGYC 2 cut(s) 28, 78
BssT1I CCWWGG 1 cut(s) 42
Bst2UI CCWGG 2 cut(s) 33, 476
Bst4CI ACNGT 1 cut(s) 155
BstACI GRCGYC 2 cut(s) 28, 78
BstAPI GCANNNNNTGC 1 cut(s) 281
BstAUI TGTACA 1 cut(s) 129
BstBAI YACGTR 1 cut(s) 65
BstC8I GCNNGC 3 cut(s) 183, 286, 300
BstDEI CTNAG 2 cut(s) 486, 507
BstDSI CCRYGG 1 cut(s) 42
BstKTI GATC 1 cut(s) 513
BstMBI GATC 1 cut(s) 510
BstMWI GCNNNNNNNGC 3 cut(s) 178, 221, 281
BstNI CCWGG 2 cut(s) 33, 476
BstNSI RCATGY 1 cut(s) 288
BstSCI CCNGG 2 cut(s) 31, 474
BstSFI CTRYAG 3 cut(s) 55, 318, 399
BstV1I GCAGC 5 cut(s) 41, 69, 282, 310, 385
BstX2I RGATCY 1 cut(s) 510
BstYI RGATCY 1 cut(s) 510
BtgI CCRYGG 1 cut(s) 42
BtsIMutI CAGTG 1 cut(s) 132
Cac8I GCNNGC 3 cut(s) 183, 286, 300
CseI GACGC 2 cut(s) 17, 86
CsiI ACCWGGT 1 cut(s) 474
Csp6I GTAC 1 cut(s) 130
CviAII CATG 3 cut(s) 43, 285, 421
CviJI RGCY 7 cut(s) 60, 73, 87, 122, 323, 398, 485
CviKI_1 RGCY 7 cut(s) 60, 73, 87, 122, 323, 398, 485
CviQI GTAC 1 cut(s) 130
DdeI CTNAG 2 cut(s) 486, 507
DpnI GATC 1 cut(s) 512
DpnII GATC 1 cut(s) 510
EciI GGCGGA 1 cut(s) 79
Ecl136II GAGCTC 1 cut(s) 73
Eco130I CCWWGG 1 cut(s) 42
Eco24I GRGCYC 1 cut(s) 75
Eco53kI GAGCTC 1 cut(s) 73
Eco57I CTGAAG 1 cut(s) 165
Eco72I CACGTG 1 cut(s) 65
EcoICRI GAGCTC 1 cut(s) 73
EcoRII CCWGG 2 cut(s) 31, 474
EcoT14I CCWWGG 1 cut(s) 42
EcoT22I ATGCAT 1 cut(s) 286
EcoT38I GRGCYC 1 cut(s) 75
ErhI CCWWGG 1 cut(s) 42
FaeI CATG 3 cut(s) 46, 288, 424
FaqI GGGAC 1 cut(s) 89
FatI CATG 3 cut(s) 42, 284, 420
FauI CCCGC 1 cut(s) 13
FauNDI CATATG 1 cut(s) 264
Fnu4HI GCNGC 5 cut(s) 55, 58, 296, 324, 399
FriOI GRGCYC 1 cut(s) 75
Fsp4HI GCNGC 5 cut(s) 55, 58, 296, 324, 399
GluI GCNGC 5 cut(s) 55, 58, 296, 324, 399
HgaI GACGC 2 cut(s) 17, 86
Hin1I GRCGYC 2 cut(s) 28, 78
Hin1II CATG 3 cut(s) 46, 288, 424
HinfI GANTC 2 cut(s) 236, 311
HphI GGTGA 2 cut(s) 306, 446
Hpy166II GTNNAC 1 cut(s) 132
Hpy188I TCNGA 1 cut(s) 508
Hpy188III TCNNGA 1 cut(s) 458
Hpy8I GTNNAC 1 cut(s) 132
Hpy99I CGWCG 3 cut(s) 80, 98, 431
HpyAV CCTTC 2 cut(s) 299, 355
HpyCH4III ACNGT 1 cut(s) 155
HpyCH4IV ACGT 2 cut(s) 64, 102
HpyCH4V TGCA 4 cut(s) 57, 275, 284, 401
HpyF10VI GCNNNNNNNGC 3 cut(s) 178, 221, 281
HpyF3I CTNAG 2 cut(s) 486, 507
HpySE526I ACGT 2 cut(s) 64, 102
Hsp92I GRCGYC 2 cut(s) 28, 78
Hsp92II CATG 3 cut(s) 46, 288, 424
Kzo9I GATC 1 cut(s) 510
LmnI GCTCC 1 cut(s) 92
LpnPI CCDG 6 cut(s) 18, 45, 216, 291, 461, 488
Lsp1109I GCAGC 5 cut(s) 41, 69, 282, 310, 385
LweI GCATC 2 cut(s) 271, 480
MabI ACCWGGT 1 cut(s) 474
MaeII ACGT 2 cut(s) 64, 102
MaeIII GTNAC 4 cut(s) 65, 312, 348, 442
MalI GATC 1 cut(s) 512
MboI GATC 1 cut(s) 510
MboII GAAGA 1 cut(s) 473
MflI RGATCY 1 cut(s) 510
MhlI GDGCHC 2 cut(s) 75, 217
MluCI AATT 2 cut(s) 206, 340
MlyI GAGTC 2 cut(s) 245, 320
MnlI CCTC 1 cut(s) 186
Mph1103I ATGCAT 1 cut(s) 286
MseI TTAA 1 cut(s) 161
MspA1I CMGCKG 1 cut(s) 295
MspR9I CCNGG 2 cut(s) 33, 476
MvaI CCWGG 2 cut(s) 33, 476
MwoI GCNNNNNNNGC 3 cut(s) 178, 221, 281
NcoI CCATGG 1 cut(s) 42
NdeI CATATG 1 cut(s) 264
NdeII GATC 1 cut(s) 510
NlaIII CATG 3 cut(s) 46, 288, 424
NlaIV GGNNCC 1 cut(s) 200
NmuCI GTSAC 2 cut(s) 65, 312
NsiI ATGCAT 1 cut(s) 286
NspI RCATGY 1 cut(s) 288
PaeI GCATGC 1 cut(s) 288
PkrI GCNGC 5 cut(s) 56, 59, 297, 325, 400
PleI GAGTC 2 cut(s) 244, 319
PmaCI CACGTG 1 cut(s) 65
PmlI CACGTG 1 cut(s) 65
PpsI GAGTC 2 cut(s) 244, 319
Ppu21I YACGTR 1 cut(s) 65
PsiI TTATAA 1 cut(s) 338
Psp124BI GAGCTC 1 cut(s) 75
Psp6I CCWGG 2 cut(s) 31, 474
PspCI CACGTG 1 cut(s) 65
PspGI CCWGG 2 cut(s) 31, 474
PspN4I GGNNCC 1 cut(s) 200
PstI CTGCAG 2 cut(s) 59, 403
PsuI RGATCY 1 cut(s) 510
RsaI GTAC 1 cut(s) 131
RsaNI GTAC 1 cut(s) 130
SacI GAGCTC 1 cut(s) 75
SaqAI TTAA 1 cut(s) 161
SatI GCNGC 5 cut(s) 55, 58, 296, 324, 399
Sau3AI GATC 1 cut(s) 510
SchI GAGTC 2 cut(s) 245, 320
ScrFI CCNGG 2 cut(s) 33, 476
SduI GDGCHC 2 cut(s) 75, 217
SexAI ACCWGGT 1 cut(s) 474
SfaNI GCATC 2 cut(s) 271, 480
SfcI CTRYAG 3 cut(s) 55, 318, 399
SphI GCATGC 1 cut(s) 288
Sse9I AATT 2 cut(s) 206, 340
SsiI CCGC 3 cut(s) 20, 90, 293
SspI AATATT 1 cut(s) 165
SstI GAGCTC 1 cut(s) 75
StyD4I CCNGG 2 cut(s) 31, 474
StyI CCWWGG 1 cut(s) 42
TaaI ACNGT 1 cut(s) 155
TaiI ACGT 2 cut(s) 67, 105
TaqI TCGA 1 cut(s) 75
TasI AATT 2 cut(s) 206, 340
TatI WGTACW 1 cut(s) 129
Tru1I TTAA 1 cut(s) 161
Tru9I TTAA 1 cut(s) 161
TscAI CASTG 1 cut(s) 139
TseFI GTSAC 2 cut(s) 65, 312
TseI GCWGC 5 cut(s) 54, 57, 295, 323, 398
Tsp45I GTSAC 2 cut(s) 65, 312
TspDTI ATGAA 1 cut(s) 264
TspRI CASTG 1 cut(s) 139
XceI RCATGY 1 cut(s) 288
Zsp2I ATGCAT 1 cut(s) 286
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.