pycom03g17940

30S ribosomal protein S20

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0001972
Physical Location & Seq
Reverse (-)
19020603 .. 19022205
1603 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 555 bp
ATGGCAGCTGCAGTTCATTGCCTATCTCCATGCCTATCCCTCCCTTCAAGATTCGGCAAGCTTTCACTCGATGGCCCTTCTACTTCCTCTGCCACTACTCATTCCACAACTTCCCTCAGTTTCTCAGCCATGCTTTCTCCCAATTTCTTCTCCAAAGGGTATTTGGCTGTGAAACCACTTCAGAGGCCGGTTCGACATTCCATTGTTTGTGAGGTCGCTACGAAGAAGCCCGACTCTGCTGTGAAGAGAGCTCGGCAGGCCGAAAAGAGGAGGATTTACAACAAATCCCGCAAATCCGAAATTAAAACCCGAATGAAGAAGGTTTGGGAAGCTCTGGATGGGCTGAAGAAGAAGCCAGATGCTGCACCCGAAGAGGTCCTTTCTATTGAGAAGCTCATTGGAGAGGCATACTCCATTATTGACAAAGCAGTAAAGGTGGGAACTCTGCACAGGAATACGGGAGCACGGAGAAAGTCTCGACTGGCTCGAAGAAAGAAGGCTGTTGAGGTCCACCATGGCTGGTACACCCCAACTCCAGAAGCTGCTAGTGTCTAA

Protein Analysis

185

Amino Acids

20.32

Weight (kDa)

10.31

Isoelectric Point (pI)

43.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S20p PF01649 78 - 164 4.3e-22 Ribosomal protein S20
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 289
AcuI CTGAAG 2 cut(s) 164, 365
AfaI GTAC 1 cut(s) 524
AfiI CCNNNNNNNGG 1 cut(s) 267
AgsI TTSAA 1 cut(s) 48
AluBI AGCT 6 cut(s) 8, 61, 251, 332, 394, 542
AluI AGCT 6 cut(s) 8, 61, 251, 332, 394, 542
Alw21I GWGCWC 2 cut(s) 253, 466
Alw26I GTCTC 1 cut(s) 480
AlwNI CAGNNNCTG 2 cut(s) 362, 542
AoxI GGCC 3 cut(s) 73, 185, 258
ApeKI GCWGC 4 cut(s) 5, 8, 362, 542
AspS9I GGNCC 3 cut(s) 74, 376, 508
AvaII GGWCC 2 cut(s) 376, 508
BanII GRGCYC 1 cut(s) 253
Bbv12I GWGCWC 2 cut(s) 253, 466
BbvI GCAGC 3 cut(s) 17, 349, 529
BccI CCATC 2 cut(s) 65, 332
BcoDI GTCTC 1 cut(s) 480
BfaI CTAG 1 cut(s) 546
BfmI CTRYAG 1 cut(s) 9
BisI GCNGC 4 cut(s) 6, 9, 363, 543
BlsI GCNGC 4 cut(s) 7, 10, 364, 544
Bme18I GGWCC 2 cut(s) 376, 508
BmgT120I GGNCC 3 cut(s) 74, 376, 508
BmsI GCATC 1 cut(s) 349
BplI GAGNNNNNCTC 4 cut(s) 395, 427, 460, 492
BpmI CTGGAG 1 cut(s) 519
BsaJI CCNNGG 1 cut(s) 514
BsaXI ACNNNNNCTCC 2 cut(s) 517, 547
Bsc4I CCNNNNNNNGG 1 cut(s) 267
Bse118I RCCGGY 1 cut(s) 187
Bse1I ACTGG 1 cut(s) 486
Bse3DI GCAATG 1 cut(s) 16
BseDI CCNNGG 1 cut(s) 514
BseGI GGATG 1 cut(s) 343
BseLI CCNNNNNNNGG 1 cut(s) 267
BseMI GCAATG 1 cut(s) 16
BseMII CTCAG 2 cut(s) 130, 138
BseNI ACTGG 1 cut(s) 486
BseRI GAGGAG 1 cut(s) 283
BseXI GCAGC 3 cut(s) 17, 349, 529
BsgI GTGCAG 2 cut(s) 348, 431
BshFI GGCC 3 cut(s) 75, 187, 260
BsiHKAI GWGCWC 2 cut(s) 253, 466
BsiSI CCGG 1 cut(s) 188
BslI CCNNNNNNNGG 1 cut(s) 267
BsmAI GTCTC 1 cut(s) 480
BsnI GGCC 3 cut(s) 75, 187, 260
Bsp1286I GDGCHC 2 cut(s) 253, 466
Bsp19I CCATGG 1 cut(s) 514
BspACI CCGC 1 cut(s) 289
BspANI GGCC 3 cut(s) 75, 187, 260
BspCNI CTCAG 2 cut(s) 129, 137
BspMAI CTGCAG 1 cut(s) 13
BsrDI GCAATG 1 cut(s) 16
BsrFI RCCGGY 1 cut(s) 187
BsrI ACTGG 1 cut(s) 486
BssAI RCCGGY 1 cut(s) 187
BssECI CCNNGG 1 cut(s) 514
BssT1I CCWWGG 1 cut(s) 514
Bst6I CTCTTC 2 cut(s) 239, 366
BstC8I GCNNGC 2 cut(s) 59, 258
BstDEI CTNAG 2 cut(s) 116, 124
BstDSI CCRYGG 1 cut(s) 514
BstF5I GGATG 1 cut(s) 343
BstMAI GTCTC 1 cut(s) 480
BstMWI GCNNNNNNNGC 1 cut(s) 257
BstSFI CTRYAG 1 cut(s) 9
BstV1I GCAGC 3 cut(s) 17, 349, 529
BsuRI GGCC 3 cut(s) 75, 187, 260
BtgI CCRYGG 1 cut(s) 514
BtsCI GGATG 1 cut(s) 343
Cac8I GCNNGC 2 cut(s) 59, 258
CaiI CAGNNNCTG 2 cut(s) 362, 542
Cfr10I RCCGGY 1 cut(s) 187
Cfr13I GGNCC 3 cut(s) 74, 376, 508
Csp6I GTAC 1 cut(s) 523
CviAII CATG 3 cut(s) 30, 130, 515
CviQI GTAC 1 cut(s) 523
DdeI CTNAG 2 cut(s) 116, 124
Eam1104I CTCTTC 2 cut(s) 239, 366
EarI CTCTTC 2 cut(s) 239, 366
Ecl136II GAGCTC 1 cut(s) 251
Eco130I CCWWGG 1 cut(s) 514
Eco24I GRGCYC 1 cut(s) 253
Eco47I GGWCC 2 cut(s) 376, 508
Eco53kI GAGCTC 1 cut(s) 251
Eco57I CTGAAG 2 cut(s) 164, 365
EcoICRI GAGCTC 1 cut(s) 251
EcoO109I RGGNCCY 1 cut(s) 376
EcoT14I CCWWGG 1 cut(s) 514
EcoT38I GRGCYC 1 cut(s) 253
ErhI CCWWGG 1 cut(s) 514
FaeI CATG 3 cut(s) 33, 133, 518
FaiI YATR 4 cut(s) 31, 131, 409, 516
FalI AAGNNNNNCTT 2 cut(s) 363, 395
FatI CATG 3 cut(s) 29, 129, 514
FauI CCCGC 1 cut(s) 296
Fnu4HI GCNGC 4 cut(s) 6, 9, 363, 543
FokI GGATG 1 cut(s) 350
FriOI GRGCYC 1 cut(s) 253
Fsp4HI GCNGC 4 cut(s) 6, 9, 363, 543
FspBI CTAG 1 cut(s) 546
GluI GCNGC 4 cut(s) 6, 9, 363, 543
GsuI CTGGAG 1 cut(s) 519
HaeIII GGCC 3 cut(s) 75, 187, 260
HapII CCGG 1 cut(s) 188
Hin1II CATG 3 cut(s) 33, 133, 518
HindIII AAGCTT 1 cut(s) 59
HinfI GANTC 2 cut(s) 51, 233
HpaII CCGG 1 cut(s) 188
Hpy166II GTNNAC 2 cut(s) 511, 525
Hpy188I TCNGA 2 cut(s) 183, 298
Hpy188III TCNNGA 4 cut(s) 48, 335, 477, 536
Hpy8I GTNNAC 2 cut(s) 511, 525
HpyAV CCTTC 4 cut(s) 54, 87, 313, 490
HpyCH4V TGCA 3 cut(s) 11, 365, 448
HpyF10VI GCNNNNNNNGC 1 cut(s) 257
HpyF3I CTNAG 2 cut(s) 116, 124
Hsp92II CATG 3 cut(s) 33, 133, 518
LmnI GCTCC 1 cut(s) 461
LpnPI CCDG 8 cut(s) 201, 242, 320, 369, 436, 467, 505, 549
Lsp1109I GCAGC 3 cut(s) 17, 349, 529
LweI GCATC 1 cut(s) 349
MaeI CTAG 1 cut(s) 546
MboII GAAGA 8 cut(s) 139, 235, 256, 328, 358, 361, 383, 501
MhlI GDGCHC 2 cut(s) 253, 466
MluCI AATT 2 cut(s) 142, 300
MlyI GAGTC 1 cut(s) 227
MseI TTAA 1 cut(s) 303
MspA1I CMGCKG 1 cut(s) 8
MspI CCGG 1 cut(s) 188
MwoI GCNNNNNNNGC 1 cut(s) 257
NcoI CCATGG 1 cut(s) 514
NlaIII CATG 3 cut(s) 33, 133, 518
NmeAIII GCCGAG 1 cut(s) 232
PcsI WCGNNNNNNNCGW 1 cut(s) 484
PfeI GAWTC 1 cut(s) 51
PkrI GCNGC 4 cut(s) 7, 10, 364, 544
PleI GAGTC 1 cut(s) 227
PpsI GAGTC 1 cut(s) 227
PpuMI RGGWCCY 1 cut(s) 376
Psp124BI GAGCTC 1 cut(s) 253
Psp5II RGGWCCY 1 cut(s) 376
PspPI GGNCC 3 cut(s) 74, 376, 508
PspPPI RGGWCCY 1 cut(s) 376
PstI CTGCAG 1 cut(s) 13
PstNI CAGNNNCTG 2 cut(s) 362, 542
PvuII CAGCTG 1 cut(s) 8
RsaI GTAC 1 cut(s) 524
RsaNI GTAC 1 cut(s) 523
SacI GAGCTC 1 cut(s) 253
SaqAI TTAA 1 cut(s) 303
SatI GCNGC 4 cut(s) 6, 9, 363, 543
Sau96I GGNCC 3 cut(s) 74, 376, 508
SchI GAGTC 1 cut(s) 227
SduI GDGCHC 2 cut(s) 253, 466
SfaNI GCATC 1 cut(s) 349
SfcI CTRYAG 1 cut(s) 9
SinI GGWCC 2 cut(s) 376, 508
Sse9I AATT 2 cut(s) 142, 300
SsiI CCGC 1 cut(s) 289
SspMI CTAG 1 cut(s) 546
SstI GAGCTC 1 cut(s) 253
StyI CCWWGG 1 cut(s) 514
TaqI TCGA 4 cut(s) 69, 193, 478, 487
TasI AATT 2 cut(s) 142, 300
TfiI GAWTC 1 cut(s) 51
Tru1I TTAA 1 cut(s) 303
Tru9I TTAA 1 cut(s) 303
TseI GCWGC 4 cut(s) 5, 8, 362, 542
TspDTI ATGAA 2 cut(s) 5, 329
TspGWI ACGGA 1 cut(s) 481
VpaK11BI GGWCC 2 cut(s) 376, 508
XcmI CCANNNNNNNNNTGG 1 cut(s) 160
XspI CTAG 1 cut(s) 546
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.